AlfalfaGEDB | Msa0675540

M. sativa cultivar zhongmu-4 / Msa0675540

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0675540	XP_039683887.1	81.295	139	26	0	1	139	1	139	1.56e-63	231
Msa0675540	XP_039683887.1	88.366	361	42	0	131	491	239	599	0.0	689

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0675540	sp\|Q9SZL8\|FRS5_ARATH	39.496	357	198	4	141	490	281	626	3.60e-84	279

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0675540	A0A1S3VJK5	39.560	91	55	0	39	129	27	117	3.70e-11	77.4
Msa0675540	A0A1S3VJK5	64.023	353	127	0	138	490	234	586	6.95e-164	492

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TFs/TRs:

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Gene ID	Type	Classification
Msa0675540	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0675540	MtrunA17_Chr5g0441581	60.278	360	143	0	131	490	216	575	4.54e-157	462
Msa0675540	MtrunA17_Chr5g0441581	43.860	114	51	2	18	129	11	113	2.84e-17	85.1
Msa0675540	MtrunA17_Chr2g0296781	53.571	364	158	3	133	490	190	548	5.17e-133	402
Msa0675540	MtrunA17_Chr6g0477261	53.571	364	158	3	133	490	79	437	6.26e-133	399
Msa0675540	MtrunA17_Chr1g0196541	50.847	354	169	3	141	491	198	549	1.87e-120	369
Msa0675540	MtrunA17_Chr5g0448761	41.737	357	190	4	141	490	272	617	1.36e-91	295
Msa0675540	MtrunA17_Chr1g0202341	41.880	351	198	3	141	490	256	601	2.32e-87	282
Msa0675540	MtrunA17_Chr2g0291581	39.266	354	209	3	141	490	262	613	1.88e-85	277
Msa0675540	MtrunA17_Chr5g0448511	37.892	351	212	2	141	490	44	389	2.16e-84	270
Msa0675540	MtrunA17_Chr2g0329031	38.251	366	210	4	130	490	199	553	2.16e-83	273

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0675540	AT4G38180.1	39.496	357	198	4	141	490	281	626	3.66e-85	279
Msa0675540	AT4G15090.1	37.293	362	220	3	130	490	246	601	3.00e-80	266
Msa0675540	AT4G15090.5	37.293	362	220	3	130	490	246	601	3.00e-80	266
Msa0675540	AT4G15090.3	37.293	362	220	3	130	490	246	601	3.00e-80	266
Msa0675540	AT4G15090.2	37.293	362	220	3	130	490	246	601	3.00e-80	266
Msa0675540	AT4G15090.4	37.293	362	220	3	130	490	246	601	3.00e-80	266
Msa0675540	AT3G22170.2	37.017	362	221	2	130	490	255	610	1.68e-77	259
Msa0675540	AT3G22170.1	37.017	362	221	2	130	490	255	610	1.68e-77	259
Msa0675540	AT1G52520.1	35.246	366	225	4	133	491	271	631	9.76e-76	252
Msa0675540	AT1G76320.3	35.897	351	216	3	141	490	194	536	1.41e-72	243

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Find 93 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGATCAGGCAGGTGTAC+GGG	0.512319	4_4:-24648492	Msa0675540:intron
AAACTAGAAACTCTAAGAAA+TGG	0.442585	4_4:-24648772	Msa0675540:CDS
AAAGGATACGCACAGTACAA+AGG	0.620281	4_4:-24647895	Msa0675540:CDS
AAAGGATGATTGATCAAGAT+AGG	0.453804	4_4:+24648983	None:intergenic
AAATGCAACTGTTATCTCGA+AGG	0.615193	4_4:-24647442	Msa0675540:CDS
AACATCACTAAAGTACTCAT+AGG	0.520771	4_4:+24648215	None:intergenic
AACTCTAAGAAATGGTCAGC+TGG	0.475783	4_4:-24648764	Msa0675540:CDS
AAGATACTAGGTCCTTTACC+TGG	0.580541	4_4:-24648077	Msa0675540:CDS
AAGATCGCAACCTAGTAAAA+TGG	0.310904	4_4:+24648110	None:intergenic
AAGTATTGTGTTGATTTCAA+CGG	0.550175	4_4:-24647343	Msa0675540:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGGATGATTGATCAAGAT+AGG	+	chr4_4:24647234-24647253	None:intergenic	30.0%
GTGAATATGCTATCAAACAA+GGG	-	chr4_4:24647409-24647428	Msa0675540:CDS	30.0%
AGATAAGTCCAAGTAAATCA+CGG	-	chr4_4:24647646-24647665	Msa0675540:CDS	30.0%
ATATTTGTTTGTCAAGTAGG+TGG	+	chr4_4:24648038-24648057	None:intergenic	30.0%
GTCATATTTGTTTGTCAAGT+AGG	+	chr4_4:24648041-24648060	None:intergenic	30.0%
TGATGATTTACACCAACAAA+CGG	+	chr4_4:24648076-24648095	None:intergenic	30.0%
TTTGTTGGTGTAAATCATCA+TGG	-	chr4_4:24648076-24648095	Msa0675540:CDS	30.0%
TTGTTGGTGTAAATCATCAT+GGG	-	chr4_4:24648077-24648096	Msa0675540:CDS	30.0%
TGATCAGTTACAATTCTCAT+TGG	+	chr4_4:24648202-24648221	None:intergenic	30.0%
AGATACAAGTAGAATTTCGT+GGG	-	chr4_4:24648749-24648768	Msa0675540:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	24647216	24649020	24647216	ID=Msa0675540;Name=Msa0675540
chr4_4	mRNA	24647216	24649020	24647216	ID=Msa0675540-mRNA-1;Parent=Msa0675540;Name=Msa0675540-mRNA-1;_AED=0.49;_eAED=0.49;_QI=0\|0\|0\|1\|1\|0.66\|3\|0\|491
chr4_4	exon	24648633	24649020	24648633	ID=Msa0675540-mRNA-1:exon:6764;Parent=Msa0675540-mRNA-1
chr4_4	exon	24648469	24648500	24648469	ID=Msa0675540-mRNA-1:exon:6763;Parent=Msa0675540-mRNA-1
chr4_4	exon	24647216	24648271	24647216	ID=Msa0675540-mRNA-1:exon:6762;Parent=Msa0675540-mRNA-1
chr4_4	CDS	24648633	24649020	24648633	ID=Msa0675540-mRNA-1:cds;Parent=Msa0675540-mRNA-1
chr4_4	CDS	24648469	24648500	24648469	ID=Msa0675540-mRNA-1:cds;Parent=Msa0675540-mRNA-1
chr4_4	CDS	24647216	24648271	24647216	ID=Msa0675540-mRNA-1:cds;Parent=Msa0675540-mRNA-1

Gene Sequence

>Msa0675540

ATGGTTGATGGTCCTCCTATCTTGATCAATCATCCTTTATTAGCCCCTGATGGAGAGCTAGATGACGGTGCTGCTCGTGTTGATGTTTCGGATTCCGGGACCTTTGAAACAACAAATGTTGTCGCAACAGATGAATTCACGCCTACTATCAATATGTCTTTTAAATCGGTTGCAGATTGTAAGTCTTTTTACCGTGAATATGCTATCAAACAAGGGTTTGCGATTAAAACTAGAAACTCTAAGAAATGGTCAGCTGGCAAGTTAAGATATTTCATTCTAACATGTGCAAGAGAAGGTCACCACGAATCGAACATACCACCAACTTTGAAGACGAATCCAACAAAGAAAGTTGATTGCTCAGCTAAGATAATGGTTGCTTTAAAGAAGGGTGTACGGGTGAACAAAAGTTTCCGTTCCATGAATATTTTCTGGGCTGATGCACGTAGTAGGTCTGCCTATGAGTACTTTAGTGATGTTGTCACGTTCGATACCACCTACTTGACAAACAAATATGACATGCCATTTGCCCCGTTTGTTGGTGTAAATCATCATGGGCAATCCATTTTACTAGGTTGCGATCTTTTATCAGCTGAAGATACTAGGTCCTTTACCTGGTTGTTCGAAACATGGCTAAGGTGTATGTCTTCGAGGCCTCCAATGAGAATTGTAACTGATCAGTGTAAAGCAATGCAGAATGCAATTCAGGTTGTGTTTCCTAATACTAGGCATAGGTGGTGTCTATGGCATATAATGAGGAAAATTACCAAAAAAGTGAAAGGATACGCACAGTACAAAGGAATAAAAAATGCCATGAAAAATTGTGTCTATGATTCTCTGCTGGTTGATGATTTTTTTGTTGGGTGGACAACATTCATTGATAAATATGGTTTGGGTGAAAATGATTGGTTGAATACCATTTTCGAAGAGCGGGAAAGATGGGTGCCATGTTATTTAAAACATGATTTCTGGGCCGGAATGTCAACAACACAGAGGAGTGAAAGTATGAATGCTTTTTTCGATGGTTACATTAATGCAAGAACTTCTCTCAGCGAATTTGTAAAGCAATACGACAACGCACTCCAAGACAAGGCTGAAAAGGAATACGAGGCAGATTTTCGGTCTTCAAGCACAATCATTCCATGCGGGTCAAATTCAACAATCGAGAGACAATTTCAAGCTGAGTACACTCATGAAAAGTATATGGAGATACAAGTAGAATTTCGTGGGAAATGCAACTGTTATCTCGAAGGTGTGACTACATTGGAATTTATATCAACATACACTGCTTTAGAGGAGAGTATTGTCTGTGGTAAACCAAACGAAAGTAAGTATTGTGTTGATTTCAACGGGGAAGACAATAATGTTATGTGCACTTGTTTGCTGTTTGAGTTCAGAGGCGTTTTGTGTAGACATTCGCTTTTCGTGCTTGGTCATCTAAGAGTGAAAGAGGTTCCGTCTAAACTTATCTTGCCATGA

Protein sequence

>Msa0675540

MVDGPPILINHPLLAPDGELDDGAARVDVSDSGTFETTNVVATDEFTPTINMSFKSVADCKSFYREYAIKQGFAIKTRNSKKWSAGKLRYFILTCAREGHHESNIPPTLKTNPTKKVDCSAKIMVALKKGVRVNKSFRSMNIFWADARSRSAYEYFSDVVTFDTTYLTNKYDMPFAPFVGVNHHGQSILLGCDLLSAEDTRSFTWLFETWLRCMSSRPPMRIVTDQCKAMQNAIQVVFPNTRHRWCLWHIMRKITKKVKGYAQYKGIKNAMKNCVYDSLLVDDFFVGWTTFIDKYGLGENDWLNTIFEERERWVPCYLKHDFWAGMSTTQRSESMNAFFDGYINARTSLSEFVKQYDNALQDKAEKEYEADFRSSSTIIPCGSNSTIERQFQAEYTHEKYMEIQVEFRGKCNCYLEGVTTLEFISTYTALEESIVCGKPNESKYCVDFNGEDNNVMCTCLLFEFRGVLCRHSLFVLGHLRVKEVPSKLILP*