AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0676070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0676070 MtrunA17_Chr3g0125491 41.279 172 45 4 18 135 87 256 8.65e-30 113
Msa0676070 MtrunA17_Chr3g0125491 33.108 148 49 4 131 228 89 236 8.66e-13 66.6
Msa0676070 MtrunA17_Chr3g0125461 41.718 163 40 3 26 135 483 643 4.67e-27 108
Msa0676070 MtrunA17_Chr3g0125461 34.375 192 60 7 89 228 446 623 2.59e-15 74.7
Msa0676070 MtrunA17_Chr3g0125431 41.718 163 40 3 26 135 465 625 1.16e-26 107
Msa0676070 MtrunA17_Chr3g0125431 37.162 148 43 4 131 228 458 605 1.61e-14 72.4
Msa0676070 MtrunA17_Chr2g0279661 40.491 163 42 3 26 135 470 630 3.76e-25 103
Msa0676070 MtrunA17_Chr3g0125441 40.491 163 42 3 26 135 495 655 9.60e-25 102
Msa0676070 MtrunA17_Chr3g0125441 36.486 148 44 5 131 228 488 635 8.88e-14 70.1
Msa0676070 MtrunA17_Chr3g0125471 41.104 163 41 3 26 135 400 560 2.51e-24 100
Msa0676070 MtrunA17_Chr3g0125471 34.375 192 60 8 89 228 363 540 1.84e-14 72.0
Msa0676070 MtrunA17_Chr3g0125451 39.264 163 44 3 26 135 460 620 5.41e-24 99.8
Msa0676070 MtrunA17_Chr3g0125451 35.135 148 46 4 131 228 453 600 1.10e-13 69.7
Msa0676070 MtrunA17_Chr1g0158211 36.420 162 51 3 26 135 188 349 7.69e-19 84.7
Msa0676070 MtrunA17_Chr1g0158201 36.420 162 51 3 26 135 450 611 3.91e-18 82.8
Msa0676070 MtrunA17_Chr2g0293081 34.356 163 52 3 26 135 435 595 5.31e-18 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0676070 AT1G16130.1 33.540 161 56 2 26 135 455 615 4.47e-18 83.2
Msa0676070 AT1G79670.1 32.727 165 56 3 26 137 460 622 1.99e-17 81.3
Msa0676070 AT1G79670.2 32.727 165 56 3 26 137 423 585 2.00e-17 81.3
Msa0676070 AT1G16160.1 31.677 161 59 2 26 135 471 631 5.15e-17 80.1
Msa0676070 AT1G69730.1 32.317 164 55 4 26 135 486 647 5.73e-16 77.0
Msa0676070 AT1G19390.1 28.866 194 85 3 26 168 490 681 9.45e-16 76.3
Msa0676070 AT1G16120.1 31.677 161 59 2 26 135 468 628 4.06e-15 74.3
Msa0676070 AT1G16110.1 32.317 164 58 3 26 136 471 634 9.87e-15 73.2

Find 34 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACATTTATATGGTTGATTT+TGG 0.093360 4_4:+26096199 Msa0676060:intron
ACTCTAATGCAAGGGATATT+TGG 0.135372 4_4:-26097216 Msa0676070:intron
TAGAAATGTGGTAATTCTTA+TGG 0.174431 4_4:-26097542 Msa0676070:CDS
ACACTTGTTTGCAAGTATTC+TGG 0.209861 4_4:+26095843 Msa0676060:intron
CCACATTTCTATGATTGATT+TGG 0.235540 4_4:+26097554 None:intergenic
CACATTTCTATGATTGATTT+GGG 0.299102 4_4:+26097555 None:intergenic
GGAGAAGTTCGTTAATAAAA+TGG 0.299762 4_4:-26096231 Msa0676070:CDS
AAAGAATCACCACATTTATA+TGG 0.306204 4_4:+26096189 Msa0676060:intron
CTCTAATGCAAGGGATATTT+GGG 0.332847 4_4:-26097215 Msa0676070:intron
CACTTGTTTGCAAGTATTCT+GGG 0.341278 4_4:+26095844 Msa0676060:intron
TCAGAATCTACTAAGAACTA+TGG 0.355941 4_4:-26098949 None:intergenic
ACTCTACTGCAGGGGACATT+GGG 0.366643 4_4:-26095876 Msa0676070:CDS
TCCATGTAATATTTAAAGAT+TGG 0.375100 4_4:+26097437 None:intergenic
CACTCTACTGCAGGGGACAT+TGG 0.379445 4_4:-26095877 Msa0676070:CDS
AGAAATGTGGTAATTCTTAT+GGG 0.420318 4_4:-26097541 Msa0676070:CDS
TCTTTACGAATTTATTGTAA+TGG 0.446969 4_4:-26097490 Msa0676070:CDS
ACCAATCTTTAAATATTACA+TGG 0.494603 4_4:-26097438 Msa0676070:CDS
TGAATAGCAAAAGGAACGGC+TGG 0.505813 4_4:-26096044 Msa0676070:intron
ACAATAAATTCGTAAAGAAG+TGG 0.515633 4_4:+26097495 None:intergenic
TCAGATAACCACTCTACTGC+AGG 0.517517 4_4:-26095886 Msa0676070:intron
TAACAAAATACACGTATGAC+AGG 0.537961 4_4:+26097377 None:intergenic
AATCTACTAAGAACTATGGT+TGG 0.544186 4_4:-26098945 Msa0676070:CDS
CAGCATTATGTAAATATGAC+GGG 0.544990 4_4:+26096018 Msa0676060:intron
GCAGCATTATGTAAATATGA+CGG 0.563347 4_4:+26096017 Msa0676060:intron
CTCTCTAAGCTACAAAACTC+AGG 0.573971 4_4:-26098892 Msa0676070:intron
ACGAACACTTTATTGACGAA+AGG 0.577677 4_4:+26097583 None:intergenic
CAGATAACCACTCTACTGCA+GGG 0.594701 4_4:-26095885 Msa0676070:CDS
GTTATCTGACTATGATCAAG+AGG 0.598670 4_4:+26095900 Msa0676060:intron
CAAAATCAACCATATAAATG+TGG 0.611139 4_4:-26096198 Msa0676070:intron
CCAAATCAATCATAGAAATG+TGG 0.613579 4_4:-26097554 Msa0676070:CDS
CAATGTCCCCTGCAGTAGAG+TGG 0.627318 4_4:+26095878 Msa0676060:intron
ATTATGAATAGCAAAAGGAA+CGG 0.628148 4_4:-26096048 Msa0676070:intron
CTCTACTGCAGGGGACATTG+GGG 0.656940 4_4:-26095875 Msa0676070:CDS
AGATAACCACTCTACTGCAG+GGG 0.678081 4_4:-26095884 Msa0676070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAATTCATATTTAAGAAAA+TGG + chr4_4:26096156-26096175 Msa0676060:intron 15.0%
!! ATATGAATTACAACAAATAT+AGG - chr4_4:26096165-26096184 Msa0676070:intron 15.0%
!!! ATGTTTCTAATCATTTTTTT+TGG - chr4_4:26096274-26096293 Msa0676070:intron 15.0%
!! GATAAAATATTAAAATAGGT+TGG + chr4_4:26097943-26097962 None:intergenic 15.0%
!! GAGTGATAAAATATTAAAAT+AGG + chr4_4:26097947-26097966 None:intergenic 15.0%
!! TATGTTTACTTCATTTATTT+AGG - chr4_4:26098526-26098545 Msa0676070:intron 15.0%
!!! TCTAAAAAGAATGATTTTTC+TGG + chr4_4:26095961-26095980 Msa0676060:intron 20.0%
!!! CAAGAAATTACTTTTCTTTT+TGG + chr4_4:26096099-26096118 Msa0676060:intron 20.0%
!!! ATACTTTTGAAGTAAATAAC+TGG - chr4_4:26096323-26096342 Msa0676070:intron 20.0%
!!! AAAATAACCAATAAGTTGAA+TGG + chr4_4:26096386-26096405 Msa0676060:intron 20.0%
!!! TTTTGTACATTCTAATTTGT+TGG - chr4_4:26096402-26096421 Msa0676070:intron 20.0%
!!! AGAAAAAACTATATTTTTGC+AGG + chr4_4:26096579-26096598 None:intergenic 20.0%
!!! TTCGTTCACATTTTTATATA+TGG + chr4_4:26097078-26097097 None:intergenic 20.0%
!! TCTTTACGAATTTATTGTAA+TGG - chr4_4:26097288-26097307 Msa0676070:intron 20.0%
!! ACCAATCTTTAAATATTACA+TGG - chr4_4:26097340-26097359 Msa0676070:intron 20.0%
!! TCCATGTAATATTTAAAGAT+TGG + chr4_4:26097344-26097363 None:intergenic 20.0%
!! ATAATAGAAAAAGAAATCGT+TGG - chr4_4:26097375-26097394 Msa0676070:intron 20.0%
!! AATAAATGTATGTATCATCT+TGG - chr4_4:26097831-26097850 Msa0676070:intron 20.0%
!! ATAAGGTAAATTTCAAAAGT+GGG + chr4_4:26097879-26097898 None:intergenic 20.0%
!! AATAAGGTAAATTTCAAAAG+TGG + chr4_4:26097880-26097899 None:intergenic 20.0%
!!! TTTTTTAAATAGAGTTTTCG+TGG - chr4_4:26098075-26098094 Msa0676070:intron 20.0%
!!! TTTTTAAATAGAGTTTTCGT+GGG - chr4_4:26098076-26098095 Msa0676070:intron 20.0%
!! TCATCTGAAACAACATTAAA+TGG + chr4_4:26096304-26096323 Msa0676060:intron 25.0%
! TTTCTTCAATGAAAAAAGAC+CGG + chr4_4:26096522-26096541 None:intergenic 25.0%
! GTTTACGAATTTATTCTCAA+TGG + chr4_4:26096959-26096978 None:intergenic 25.0%
!! TAGAAATGTGGTAAAACTTT+TGG + chr4_4:26097001-26097020 None:intergenic 25.0%
!! CACATTTCTATGATTGATTT+GGG - chr4_4:26097011-26097030 Msa0676070:intron 25.0%
!! TCGTTATTTCATCAATGAAA+TGG + chr4_4:26097046-26097065 None:intergenic 25.0%
!!! TATGTTCACTTCATTGATTT+AGG - chr4_4:26097170-26097189 Msa0676070:CDS 25.0%
!! CACATTTCTATGATTGATTT+GGG + chr4_4:26097011-26097030 None:intergenic 25.0%
! TAGAAATGTGGTAATTCTTA+TGG - chr4_4:26097236-26097255 Msa0676070:intron 25.0%
! AGAAATGTGGTAATTCTTAT+GGG - chr4_4:26097237-26097256 Msa0676070:intron 25.0%
! ACAATAAATTCGTAAAGAAG+TGG + chr4_4:26097286-26097305 None:intergenic 25.0%
!!! TTTTTTACTTTGGAGCTTTA+AGG - chr4_4:26097503-26097522 Msa0676070:CDS 25.0%
! TGTTGTATGATTATGATCAA+GGG + chr4_4:26097542-26097561 None:intergenic 25.0%
! TTGTTGTATGATTATGATCA+AGG + chr4_4:26097543-26097562 None:intergenic 25.0%
!!! TATGTTTTTGCAATGTACAT+TGG - chr4_4:26097689-26097708 Msa0676070:intron 25.0%
! ACATTATGGACAAAATTGAT+GGG - chr4_4:26097741-26097760 Msa0676070:intron 25.0%
!! GTTTCTGTATTTAAAGTAGT+CGG - chr4_4:26097787-26097806 Msa0676070:intron 25.0%
! TCAATATAAGCGTTAAAGAT+TGG - chr4_4:26098266-26098285 Msa0676070:intron 25.0%
!!! ATTGAAAGGTGTTAAACTTT+TGG + chr4_4:26098365-26098384 None:intergenic 25.0%
! TAGATTCATTCACATTTCTA+TGG + chr4_4:26098442-26098461 None:intergenic 25.0%
!!! CACATTTATATGGTTGATTT+TGG + chr4_4:26098582-26098601 None:intergenic 25.0%
! CAAAATCAACCATATAAATG+TGG - chr4_4:26098580-26098599 Msa0676070:intron 25.0%
! AAAGAATCACCACATTTATA+TGG + chr4_4:26098592-26098611 None:intergenic 25.0%
!! TATAAATGTGGTGATTCTTT+TGG - chr4_4:26098592-26098611 Msa0676070:intron 25.0%
!! ATAAATGTGGTGATTCTTTT+GGG - chr4_4:26098593-26098612 Msa0676070:intron 25.0%
! ACATGATTATGAATAGCAAA+AGG - chr4_4:26098725-26098744 Msa0676070:intron 25.0%
! ATTATGAATAGCAAAAGGAA+CGG - chr4_4:26098730-26098749 Msa0676070:intron 25.0%
AATCTACTAAGAACTATGGT+TGG - chr4_4:26095833-26095852 Msa0676070:CDS 30.0%
! AATTGATAGTTGCAAATGAC+TGG + chr4_4:26096209-26096228 Msa0676060:intron 30.0%
CATTTGCAACTATCAATTTG+TGG - chr4_4:26096211-26096230 Msa0676070:CDS 30.0%
TGAATGTTTCTCCTATTGTA+TGG - chr4_4:26096347-26096366 Msa0676070:intron 30.0%
TCTTGATCCATTCAACTTAT+TGG - chr4_4:26096376-26096395 Msa0676070:intron 30.0%
AAGAAACAAAGTACATTGCA+AGG - chr4_4:26096536-26096555 Msa0676070:intron 30.0%
AGTGGTTATCTATGATTAAG+AGG - chr4_4:26096714-26096733 Msa0676070:intron 30.0%
AGATCACAATCTCATTACAA+AGG + chr4_4:26096769-26096788 None:intergenic 30.0%
TTGTAATGAGATTGTGATCT+AGG - chr4_4:26096769-26096788 Msa0676070:intron 30.0%
ACAATGGCAAAAGAAACTAT+TGG + chr4_4:26096872-26096891 None:intergenic 30.0%
TTGGAATACAAGAATGACAA+TGG + chr4_4:26096888-26096907 None:intergenic 30.0%
!! ACGAATCTTTAAAGCTTACT+TGG + chr4_4:26096907-26096926 None:intergenic 30.0%
CCAAATCAATCATAGAAATG+TGG + chr4_4:26097224-26097243 None:intergenic 30.0%
CCACATTTCTATGATTGATT+TGG - chr4_4:26097010-26097029 Msa0676070:intron 30.0%
TTGAGTCGTTCATCAATAAT+TGG + chr4_4:26097106-26097125 None:intergenic 30.0%
CCACATTTCTATGATTGATT+TGG + chr4_4:26097010-26097029 None:intergenic 30.0%
CCAAATCAATCATAGAAATG+TGG - chr4_4:26097224-26097243 Msa0676070:intron 30.0%
TAACAAAATACACGTATGAC+AGG + chr4_4:26097404-26097423 None:intergenic 30.0%
GTTGTATGATTATGATCAAG+GGG + chr4_4:26097541-26097560 None:intergenic 30.0%
ATACAACAACTCTAATGCAA+GGG - chr4_4:26097554-26097573 Msa0676070:CDS 30.0%
!!! CACTTGTTTTGAAGGATTTT+GGG + chr4_4:26097598-26097617 None:intergenic 30.0%
!!! ACACTTGTTTTGAAGGATTT+TGG + chr4_4:26097599-26097618 None:intergenic 30.0%
!!! TGTTAAGACACTTGTTTTGA+AGG + chr4_4:26097606-26097625 None:intergenic 30.0%
!! GAGTGATGTCTATAGTTTTA+CGG - chr4_4:26097631-26097650 Msa0676070:intron 30.0%
AGACTGTTTACTTCACATTA+TGG - chr4_4:26097727-26097746 Msa0676070:intron 30.0%
CACATTATGGACAAAATTGA+TGG - chr4_4:26097740-26097759 Msa0676070:intron 30.0%
GGAAAATATTGAGCATCTAA+AGG - chr4_4:26097762-26097781 Msa0676070:intron 30.0%
AGAAAAGCAAAAGAAACTGT+TGG + chr4_4:26098235-26098254 None:intergenic 30.0%
ACTTACGAACTTATTCACAA+TGG + chr4_4:26098322-26098341 None:intergenic 30.0%
TCGTTCTTTCATCAATGAAA+TGG + chr4_4:26098409-26098428 None:intergenic 30.0%
! TGAATCTAATCGTTGATGAA+CGG - chr4_4:26098454-26098473 Msa0676070:intron 30.0%
TAACCATAATTGGTGTGATT+TGG - chr4_4:26098484-26098503 Msa0676070:intron 30.0%
AACCATAATTGGTGTGATTT+GGG - chr4_4:26098485-26098504 Msa0676070:intron 30.0%
GGAGAAGTTCGTTAATAAAA+TGG - chr4_4:26098547-26098566 Msa0676070:intron 30.0%
!! ACCAATCTTTAAAGCTTACA+TGG - chr4_4:26098699-26098718 Msa0676070:intron 30.0%
!! TCCATGTAAGCTTTAAAGAT+TGG + chr4_4:26098703-26098722 None:intergenic 30.0%
CAGCATTATGTAAATATGAC+GGG + chr4_4:26098763-26098782 None:intergenic 30.0%
GCAGCATTATGTAAATATGA+CGG + chr4_4:26098764-26098783 None:intergenic 30.0%
!!! AAAGTTTTTGACTTGCTTCA+AGG - chr4_4:26098849-26098868 Msa0676070:intron 30.0%
CAAGAACATGTCCATACAAT+AGG + chr4_4:26096361-26096380 Msa0676060:intron 35.0%
AAGACCAGTTACTTCAAGTA+AGG - chr4_4:26096453-26096472 Msa0676070:intron 35.0%
GTTTCCTTACTTGAAGTAAC+TGG + chr4_4:26096460-26096479 Msa0676060:CDS 35.0%
AGACCGGTTACTTCATATAA+GGG + chr4_4:26096506-26096525 None:intergenic 35.0%
AAGACCGGTTACTTCATATA+AGG + chr4_4:26096507-26096526 None:intergenic 35.0%
!! ACTATATTTTTGCAGGCTAG+TGG + chr4_4:26096572-26096591 None:intergenic 35.0%
! AGACTGATGTCTATGGTTTT+GGG + chr4_4:26096630-26096649 None:intergenic 35.0%
! AAGACTGATGTCTATGGTTT+TGG + chr4_4:26096631-26096650 None:intergenic 35.0%
CCATAATACTTGCAAACAAG+CGG + chr4_4:26096664-26096683 None:intergenic 35.0%
CGCTTGTTTGCAAGTATTAT+GGG - chr4_4:26096662-26096681 Msa0676070:intron 35.0%
ATCCTTGAAGATACAAAGTC+AGG - chr4_4:26096741-26096760 Msa0676070:intron 35.0%
TTCCTGACTTTGTATCTTCA+AGG + chr4_4:26096746-26096765 None:intergenic 35.0%
TTCACATCTCTACGTATGAT+TGG - chr4_4:26096807-26096826 Msa0676070:intron 35.0%
! CAGTTGAATGAAAGTATGCT+AGG - chr4_4:26096838-26096857 Msa0676070:intron 35.0%
! GAATGAAAGTATGCTAGGAT+AGG - chr4_4:26096843-26096862 Msa0676070:intron 35.0%
CGACTCAATCAATCCATAAT+TGG - chr4_4:26097118-26097137 Msa0676070:CDS 35.0%
AATCCATAATTGGTGCGATT+TGG - chr4_4:26097128-26097147 Msa0676070:CDS 35.0%
ATCCATAATTGGTGCGATTT+GGG - chr4_4:26097129-26097148 Msa0676070:CDS 35.0%
ACGAACACTTTATTGACGAA+AGG + chr4_4:26097198-26097217 None:intergenic 35.0%
TCACATCTCTATGCAAGATT+GGG + chr4_4:26097435-26097454 None:intergenic 35.0%
TTCACATCTCTATGCAAGAT+TGG + chr4_4:26097436-26097455 None:intergenic 35.0%
CATACAACAACTCTAATGCA+AGG - chr4_4:26097553-26097572 Msa0676070:CDS 35.0%
ACTCTAATGCAAGGGATATT+TGG - chr4_4:26097562-26097581 Msa0676070:CDS 35.0%
CTCTAATGCAAGGGATATTT+GGG - chr4_4:26097563-26097582 Msa0676070:CDS 35.0%
! TGTATCATCTTGGTCTTCAT+CGG - chr4_4:26097841-26097860 Msa0676070:intron 35.0%
GGTACTTATTTGACAGGAAA+TGG + chr4_4:26097922-26097941 None:intergenic 35.0%
!! TAGGTTGGTACTTATTTGAC+AGG + chr4_4:26097928-26097947 None:intergenic 35.0%
! TAAATAGAGTTTTCGTGGGT+AGG - chr4_4:26098080-26098099 Msa0676070:intron 35.0%
! AAATAGAGTTTTCGTGGGTA+GGG - chr4_4:26098081-26098100 Msa0676070:intron 35.0%
ACACCTTTCAATGACTGATT+TGG - chr4_4:26098373-26098392 Msa0676070:intron 35.0%
! CACCTTTCAATGACTGATTT+GGG - chr4_4:26098374-26098393 Msa0676070:intron 35.0%
TTCCCAAATCAGTCATTGAA+AGG + chr4_4:26098379-26098398 None:intergenic 35.0%
ACCATAATTGGTGTGATTTG+GGG - chr4_4:26098486-26098505 Msa0676070:intron 35.0%
GTTATCTGACTATGATCAAG+AGG + chr4_4:26098881-26098900 None:intergenic 35.0%
CACTTGTTTGCAAGTATTCT+GGG + chr4_4:26098937-26098956 None:intergenic 35.0%
ACACTTGTTTGCAAGTATTC+TGG + chr4_4:26098938-26098957 None:intergenic 35.0%
!! CTGAGTTTTGTAGCTTAGAG+AGG + chr4_4:26095888-26095907 Msa0676060:intron 40.0%
CTCTCTAAGCTACAAAACTC+AGG - chr4_4:26095886-26095905 Msa0676070:intron 40.0%
GGGGAAATTTGAGCATCTAA+AGG + chr4_4:26096485-26096504 Msa0676060:CDS 40.0%
TCCCCCTTATATGAAGTAAC+CGG - chr4_4:26096500-26096519 Msa0676070:intron 40.0%
ACCGGTTACTTCATATAAGG+GGG + chr4_4:26096504-26096523 None:intergenic 40.0%
GACCGGTTACTTCATATAAG+GGG + chr4_4:26096505-26096524 None:intergenic 40.0%
TTCGTGTGAATAGATCTGCT+AGG - chr4_4:26096599-26096618 Msa0676070:intron 40.0%
!! GACTGATGTCTATGGTTTTG+GGG + chr4_4:26096629-26096648 None:intergenic 40.0%
ACAGAGAAGACTGATGTCTA+TGG + chr4_4:26096637-26096656 None:intergenic 40.0%
CCGCTTGTTTGCAAGTATTA+TGG - chr4_4:26096661-26096680 Msa0676070:intron 40.0%
TCCATAATTGGTGCGATTTG+GGG - chr4_4:26097130-26097149 Msa0676070:CDS 40.0%
! GTATCATCTTGGTCTTCATC+GGG - chr4_4:26097842-26097861 Msa0676070:intron 40.0%
! AATAGAGTTTTCGTGGGTAG+GGG - chr4_4:26098082-26098101 Msa0676070:intron 40.0%
ATCATGGCATACTTGCACTT+AGG + chr4_4:26098206-26098225 None:intergenic 40.0%
AAGTGCAAGTATGCCATGAT+AGG - chr4_4:26098206-26098225 Msa0676070:intron 40.0%
AAACTGTTGGACTCCTATCA+TGG + chr4_4:26098222-26098241 None:intergenic 40.0%
CGGCTCAATCTAACCATAAT+TGG - chr4_4:26098474-26098493 Msa0676070:intron 40.0%
TCCCCAAATCACACCAATTA+TGG + chr4_4:26098490-26098509 None:intergenic 40.0%
! CCATAATTGGTGCGATTTGG+GGG - chr4_4:26097131-26097150 Msa0676070:CDS 45.0%
CTTGTAGATCACAATCTCGC+TGG - chr4_4:26097469-26097488 Msa0676070:CDS 45.0%
! ATAGAGTTTTCGTGGGTAGG+GGG - chr4_4:26098083-26098102 Msa0676070:intron 45.0%
TGAATAGCAAAAGGAACGGC+TGG - chr4_4:26098734-26098753 Msa0676070:intron 45.0%
TCAGATAACCACTCTACTGC+AGG - chr4_4:26098892-26098911 Msa0676070:intron 45.0%
CAGATAACCACTCTACTGCA+GGG - chr4_4:26098893-26098912 Msa0676070:CDS 45.0%
AGATAACCACTCTACTGCAG+GGG - chr4_4:26098894-26098913 Msa0676070:CDS 45.0%
!! AAAATAATACAAATATATTA+TGG + chr4_4:26096126-26096145 Msa0676060:intron 5.0%
!!! ATATTTTTTTTTTTTTACTT+TGG - chr4_4:26097493-26097512 Msa0676070:CDS 5.0%
!! TAAGATTATAAAATTTAATA+AGG + chr4_4:26097896-26097915 None:intergenic 5.0%
!!! TTAAATTAAACTTTTTTTAA+AGG + chr4_4:26098002-26098021 None:intergenic 5.0%
CTGAAGCGCACATGACGTTT+GGG + chr4_4:26096696-26096715 None:intergenic 50.0%
ACTGAAGCGCACATGACGTT+TGG + chr4_4:26096697-26096716 None:intergenic 50.0%
AAACGTCATGTGCGCTTCAG+TGG - chr4_4:26096696-26096715 Msa0676070:intron 50.0%
CCCCCAAATCGCACCAATTA+TGG + chr4_4:26097134-26097153 None:intergenic 50.0%
TTGCACTTAGGCATGGCAAC+GGG + chr4_4:26098194-26098213 None:intergenic 50.0%
CTTGCACTTAGGCATGGCAA+CGG + chr4_4:26098195-26098214 None:intergenic 50.0%
GGCATACTTGCACTTAGGCA+TGG + chr4_4:26098201-26098220 None:intergenic 50.0%
ACTCTACTGCAGGGGACATT+GGG - chr4_4:26098902-26098921 Msa0676070:CDS 50.0%
TTAGGCATGGCAACGGGGAT+GGG + chr4_4:26098188-26098207 None:intergenic 55.0%
TGCACTTAGGCATGGCAACG+GGG + chr4_4:26098193-26098212 None:intergenic 55.0%
CAATGTCCCCTGCAGTAGAG+TGG + chr4_4:26098903-26098922 None:intergenic 55.0%
CACTCTACTGCAGGGGACAT+TGG - chr4_4:26098901-26098920 Msa0676070:CDS 55.0%
CTCTACTGCAGGGGACATTG+GGG - chr4_4:26098903-26098922 Msa0676070:CDS 55.0%
!! TTTTCGTGGGTAGGGGGTGC+CGG - chr4_4:26098089-26098108 Msa0676070:intron 60.0%
!! GGGTGCCGGGAATCCTGTTT+GGG - chr4_4:26098103-26098122 Msa0676070:intron 60.0%
! GGTGCCGGGAATCCTGTTTG+GGG - chr4_4:26098104-26098123 Msa0676070:intron 60.0%
CCGTCCCCAAACAGGATTCC+CGG + chr4_4:26098111-26098130 None:intergenic 60.0%
CCGGGAATCCTGTTTGGGGA+CGG - chr4_4:26098108-26098127 Msa0676070:intron 60.0%
CGGGAATCCTGTTTGGGGAC+GGG - chr4_4:26098109-26098128 Msa0676070:intron 60.0%
GGGAATCCTGTTTGGGGACG+GGG - chr4_4:26098110-26098129 Msa0676070:intron 60.0%
GGAATCCTGTTTGGGGACGG+GGG - chr4_4:26098111-26098130 Msa0676070:intron 60.0%
CTTAGGCATGGCAACGGGGA+TGG + chr4_4:26098189-26098208 None:intergenic 60.0%
!! TTTCGTGGGTAGGGGGTGCC+GGG - chr4_4:26098090-26098109 Msa0676070:intron 65.0%
!! GGGGTGCCGGGAATCCTGTT+TGG - chr4_4:26098102-26098121 Msa0676070:intron 65.0%
CAGGGGTGGGTGCATGGAAG+CGG - chr4_4:26098134-26098153 Msa0676070:intron 65.0%
AGGGGTGGGTGCATGGAAGC+GGG - chr4_4:26098135-26098154 Msa0676070:intron 65.0%
ATGGCAACGGGGATGGGTCG+GGG + chr4_4:26098182-26098201 None:intergenic 65.0%
CATGGCAACGGGGATGGGTC+GGG + chr4_4:26098183-26098202 None:intergenic 65.0%
GCATGGCAACGGGGATGGGT+CGG + chr4_4:26098184-26098203 None:intergenic 65.0%
TCCTGTTTGGGGACGGGGGC+AGG - chr4_4:26098115-26098134 Msa0676070:intron 70.0%
CCTGTTTGGGGACGGGGGCA+GGG - chr4_4:26098116-26098135 Msa0676070:intron 70.0%
CTGTTTGGGGACGGGGGCAG+GGG - chr4_4:26098117-26098136 Msa0676070:intron 70.0%
GGGGTGGGTGCATGGAAGCG+GGG - chr4_4:26098136-26098155 Msa0676070:intron 70.0%
GGGTGGGTGCATGGAAGCGG+GGG - chr4_4:26098137-26098156 Msa0676070:intron 70.0%
TGGGTGCATGGAAGCGGGGG+CGG - chr4_4:26098140-26098159 Msa0676070:intron 70.0%
TGGCAACGGGGATGGGTCGG+GGG + chr4_4:26098181-26098200 None:intergenic 70.0%
CCCTGCCCCCGTCCCCAAAC+AGG + chr4_4:26098119-26098138 None:intergenic 75.0%
TTTGGGGACGGGGGCAGGGG+TGG - chr4_4:26098120-26098139 Msa0676070:intron 75.0%
TTGGGGACGGGGGCAGGGGT+GGG - chr4_4:26098121-26098140 Msa0676070:intron 75.0%
AACGGGGATGGGTCGGGGGC+AGG + chr4_4:26098177-26098196 None:intergenic 75.0%
! CGGGGGCAGGGGTGGGTGCA+TGG - chr4_4:26098128-26098147 Msa0676070:intron 80.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 26095833 26098967 26095833 ID=Msa0676070;Name=Msa0676070
chr4_4 mRNA 26095833 26098967 26095833 ID=Msa0676070-mRNA-1;Parent=Msa0676070;Name=Msa0676070-mRNA-1;_AED=0.28;_eAED=0.37;_QI=0|0|0|0.83|0|0.16|6|0|228
chr4_4 exon 26098893 26098967 26098893 ID=Msa0676070-mRNA-1:exon:7007;Parent=Msa0676070-mRNA-1
chr4_4 exon 26097390 26097608 26097390 ID=Msa0676070-mRNA-1:exon:7006;Parent=Msa0676070-mRNA-1
chr4_4 exon 26097109 26097225 26097109 ID=Msa0676070-mRNA-1:exon:7005;Parent=Msa0676070-mRNA-1
chr4_4 exon 26096199 26096252 26096199 ID=Msa0676070-mRNA-1:exon:7004;Parent=Msa0676070-mRNA-1
chr4_4 exon 26095910 26096059 26095910 ID=Msa0676070-mRNA-1:exon:7003;Parent=Msa0676070-mRNA-1
chr4_4 exon 26095833 26095904 26095833 ID=Msa0676070-mRNA-1:exon:7002;Parent=Msa0676070-mRNA-1
chr4_4 CDS 26098893 26098967 26098893 ID=Msa0676070-mRNA-1:cds;Parent=Msa0676070-mRNA-1
chr4_4 CDS 26097390 26097608 26097390 ID=Msa0676070-mRNA-1:cds;Parent=Msa0676070-mRNA-1
chr4_4 CDS 26097109 26097225 26097109 ID=Msa0676070-mRNA-1:cds;Parent=Msa0676070-mRNA-1
chr4_4 CDS 26096199 26096252 26096199 ID=Msa0676070-mRNA-1:cds;Parent=Msa0676070-mRNA-1
chr4_4 CDS 26095910 26096059 26095910 ID=Msa0676070-mRNA-1:cds;Parent=Msa0676070-mRNA-1
chr4_4 CDS 26095833 26095904 26095833 ID=Msa0676070-mRNA-1:cds;Parent=Msa0676070-mRNA-1
Gene Sequence

>Msa0676070

AATCTACTAAGAACTATGGTTGGCTATAATAGTGAAAATCACTGTTTTATCTCCTCTCTAAGCTACAAAACTCAGGAGCCTTTCGTCAATAAAGTGTTCGTTCTTTCCCAAATCAATCATAGAAATGTGGTAATTCTTATGGGTTGTTTTTTTAGAGAAAAAAGATCCACTTCTTTACGAATTTATTGTAATGGTAGTGTTTATTATCGTCATCATGATCAAAACCAATCTTTAAATATTACATGGAATACAAGATTGATAATAGAAAAAGAAATCGTTGGAGTCCTGTCATACGGATATTTGGGTATCTTTGACCCAAAATCCTTCAAAACAAGTGTCTTAACAGAGAAGAGTGATGTCTATAGTTTTACGGTTCTCTTAGCAGAGCTACTCACAGAAAGAAAACAATATGAGAAGTTCGTTAATAAAATGGTCGTTCTTTCCAAAATCAACCATATAAATGTGCAAAAGGAACGGCTGGAGTCCCGTCATATTTACATAATGCTGCTTCTACATCAAATCTTACATGATGTTAAATTTACAAATATACTCTTAGATCACAATCTTACTGCAAAAGTTTTTGACTTGCTTCAAGGATTGTTCCTCTTGATCATAATAACCACTCTACTGCAGGGGACATTGGGGTATCTTGACCCAGAATACTTGCAAACAAGTGTCTTAACATAG
Protein sequence

>Msa0676070

NLLRTMVGYNSENHCFISSLSYKTQEPFVNKVFVLSQINHRNVVILMGCFFREKRSTSLRIYCNGSVYYRHHDQNQSLNITWNTRLIIEKEIVGVLSYGYLGIFDPKSFKTSVLTEKSDVYSFTVLLAELLTERKQYEKFVNKMVVLSKINHINVQKERLESRHIYIMLLLHQILHDVKFTNILLDHNLTAKVFDLLQGLFLLIIITTLLQGTLGYLDPEYLQTSVLT*