AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0678640


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0678640 MtrunA17_Chr4g0024181 86.486 74 10 0 1 74 1 74 3.76e-43 134
Msa0678640 MtrunA17_Chr2g0322831 68.000 50 16 0 24 73 30 79 8.75e-20 75.1
Msa0678640 MtrunA17_Chr2g0288361 48.000 75 36 2 1 73 1 74 1.90e-17 69.3
Msa0678640 MtrunA17_Chr5g0396771 63.043 46 17 0 26 71 37 82 5.78e-17 68.6
Msa0678640 MtrunA17_Chr7g0229101 57.143 42 18 0 27 68 29 70 3.27e-13 61.2
Msa0678640 MtrunA17_Chr7g0240821 54.545 44 20 0 28 71 59 102 1.62e-12 59.7
Msa0678640 MtrunA17_Chr4g0044421 56.098 41 18 0 28 68 70 110 2.37e-12 58.9
Msa0678640 MtrunA17_Chr4g0037141 58.537 41 17 0 28 68 70 110 3.07e-12 59.7
Msa0678640 MtrunA17_Chr4g0055981 60.976 41 16 0 28 68 113 153 4.68e-12 58.5
Msa0678640 MtrunA17_Chr5g0435101 53.659 41 19 0 28 68 69 109 7.91e-12 57.8
Msa0678640 MtrunA17_Chr8g0360111 56.098 41 18 0 28 68 83 123 9.12e-12 58.2
Msa0678640 MtrunA17_Chr7g0220341 56.098 41 18 0 28 68 85 125 1.47e-11 57.8
Msa0678640 MtrunA17_Chr3g0121241 54.762 42 19 0 27 68 99 140 1.72e-11 57.4
Msa0678640 MtrunA17_Chr4g0070641 56.098 41 18 0 28 68 70 110 1.85e-11 56.2
Msa0678640 MtrunA17_Chr8g0346561 56.098 41 18 0 28 68 70 110 2.30e-11 57.0
Msa0678640 MtrunA17_Chr4g0070611 56.098 41 18 0 28 68 70 110 3.22e-11 56.6
Msa0678640 MtrunA17_Chr5g0434901 51.220 41 20 0 28 68 31 71 4.85e-11 53.9
Msa0678640 MtrunA17_Chr7g0228991 51.220 41 20 0 28 68 87 127 5.16e-11 56.2
Msa0678640 MtrunA17_Chr7g0275911 54.545 44 20 0 28 71 70 113 5.86e-11 55.8
Msa0678640 MtrunA17_Chr1g0152011 52.381 42 20 0 28 69 71 112 6.51e-11 55.8
Msa0678640 MtrunA17_Chr3g0135311 50.000 42 21 0 28 69 71 112 6.68e-11 55.8
Msa0678640 MtrunA17_Chr7g0229131 47.619 42 22 0 27 68 29 70 8.10e-11 54.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0678640 AT2G46410.1 63.830 47 17 0 26 72 36 82 1.52e-16 67.8
Msa0678640 AT1G01380.1 65.854 41 14 0 26 66 32 72 2.25e-15 64.7
Msa0678640 AT5G53200.1 56.522 46 20 0 26 71 31 76 9.19e-14 60.8
Msa0678640 AT4G01060.3 60.976 41 16 0 26 66 31 71 1.89e-13 59.3
Msa0678640 AT4G01060.1 60.976 41 16 0 26 66 33 73 2.01e-13 59.3
Msa0678640 AT4G01060.2 60.976 41 16 0 26 66 30 70 2.09e-13 59.3
Msa0678640 AT1G22640.3 58.537 41 17 0 28 68 13 53 3.30e-12 58.9
Msa0678640 AT2G30420.1 54.348 46 21 0 26 71 38 83 3.74e-12 57.0
Msa0678640 AT4G38620.1 58.537 41 17 0 28 68 70 110 5.17e-12 59.3
Msa0678640 AT1G22640.1 58.537 41 17 0 28 68 70 110 6.83e-12 58.9
Msa0678640 AT5G35550.1 58.537 41 17 0 28 68 72 112 1.63e-11 57.8
Msa0678640 AT5G14750.4 50.000 44 22 0 28 71 13 56 2.72e-11 55.5
Msa0678640 AT5G14750.2 50.000 44 22 0 28 71 13 56 2.72e-11 55.5
Msa0678640 AT4G09460.1 51.220 41 20 0 28 68 70 110 2.73e-11 57.0
Msa0678640 AT3G49690.1 52.381 42 20 0 28 69 71 112 4.22e-11 57.0
Msa0678640 AT5G14750.3 50.000 44 22 0 28 71 40 83 5.33e-11 55.5
Msa0678640 AT3G27920.1 47.917 48 25 0 24 71 68 115 6.00e-11 55.8
Msa0678640 AT5G65790.1 52.381 42 20 0 28 69 71 112 7.01e-11 56.2
Msa0678640 AT2G30424.1 46.154 52 25 1 26 74 34 85 8.17e-11 53.5
Msa0678640 AT3G13540.1 56.098 41 18 0 28 68 81 121 8.85e-11 55.8
Msa0678640 AT2G30432.1 50.000 46 23 0 26 71 33 78 9.07e-11 52.8
Msa0678640 AT5G35550.2 60.000 40 16 0 29 68 76 115 9.19e-11 55.8
Msa0678640 AT5G14750.1 50.000 44 22 0 28 71 74 117 9.34e-11 55.1

Find 27 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTCTTCTGCTGTTCTTCC+AGG 0.213845 4_4:-34722327 None:intergenic
ACTACAAATAGAGAAGAAAT+AGG 0.260769 4_4:-34721476 None:intergenic
CAATGGTTTATAATCTTGTT+GGG 0.335018 4_4:+34721953 Msa0678640:CDS
AAGTAAATTATTGTTAATAT+AGG 0.342014 4_4:-34721526 None:intergenic
TTGATTGCTGGAAGAATTCC+TGG 0.342209 4_4:+34722309 Msa0678640:CDS
TGAGTGGTGTATTAATATAT+AGG 0.379554 4_4:-34721552 None:intergenic
CATAGGTGGTCATTGATTGC+TGG 0.399125 4_4:+34722297 Msa0678640:intron
ACAATGGTTTATAATCTTGT+TGG 0.399798 4_4:+34721952 Msa0678640:CDS
TTTCTTGATTCATTCATAGT+TGG 0.417658 4_4:-34722394 None:intergenic
TCAACCTTTGAACCTTGGTC+TGG 0.418134 4_4:-34721901 None:intergenic
AGAGAAGAAATAGGGAAGAC+GGG 0.426905 4_4:-34721467 None:intergenic
AATCAAGATGTGGGAAACTA+TGG 0.433656 4_4:-34721608 None:intergenic
CTACAAATAGAGAAGAAATA+GGG 0.442382 4_4:-34721475 None:intergenic
AAAATTCAACCTTTGAACCT+TGG 0.444402 4_4:-34721906 None:intergenic
GTATTTGATAATCAAGATGT+GGG 0.495195 4_4:-34721617 None:intergenic
TTTATAATCTTGTTGGGGAG+AGG 0.499577 4_4:+34721959 Msa0678640:CDS
AGAGAAAATGCTTATTACAA+TGG 0.512584 4_4:+34721936 Msa0678640:CDS
TGTATTTGATAATCAAGATG+TGG 0.524459 4_4:-34721618 None:intergenic
TGAATGAATCAAGAAATTTG+TGG 0.536313 4_4:+34722401 Msa0678640:three_prime_UTR
AACTTAATTTGCTATTGCAA+TGG 0.540183 4_4:+34721655 Msa0678640:exon
TAGAGAAGAAATAGGGAAGA+CGG 0.548087 4_4:-34721468 None:intergenic
GCAACCAGACCAAGGTTCAA+AGG 0.564494 4_4:+34721897 Msa0678640:CDS
AATGGTTTATAATCTTGTTG+GGG 0.582716 4_4:+34721954 Msa0678640:CDS
AATGTTTCAACAGATTCCAA+AGG 0.613753 4_4:+34721701 Msa0678640:CDS
GAGAAGAAATAGGGAAGACG+GGG 0.635275 4_4:-34721466 None:intergenic
ACAGATCAGCAACCAGACCA+AGG 0.650716 4_4:+34721889 Msa0678640:intron
TGGAATGATTGAGATTTGAG+TGG 0.693035 4_4:-34721568 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTAAATTATTGTTAATAT+AGG - chr4_4:34721529-34721548 None:intergenic 10.0%
!! AAGTAAATTATTGTTAATAT+AGG - chr4_4:34721529-34721548 None:intergenic 10.0%
!!! AATACTTTTTTTATACACTA+AGG + chr4_4:34721788-34721807 Msa0678640:intron 15.0%
!! TACCATTAAAAAATAATTAC+TGG - chr4_4:34722118-34722137 None:intergenic 15.0%
!!! AATACTTTTTTTATACACTA+AGG + chr4_4:34721788-34721807 Msa0678640:intron 15.0%
!! TACCATTAAAAAATAATTAC+TGG - chr4_4:34722118-34722137 None:intergenic 15.0%
!!! CTTTTCTCTTAATCAATATA+TGG - chr4_4:34721858-34721877 None:intergenic 20.0%
!!! CATATATTGATTAAGAGAAA+AGG + chr4_4:34721856-34721875 Msa0678640:intron 20.0%
!! ATAACATCAATAACAAAGTT+TGG - chr4_4:34722033-34722052 None:intergenic 20.0%
!!! CACCAGTAATTATTTTTTAA+TGG + chr4_4:34722113-34722132 Msa0678640:intron 20.0%
!! TATGAATGAATCAAGAAATT+TGG + chr4_4:34722399-34722418 Msa0678640:three_prime_UTR 20.0%
!!! CTTTTCTCTTAATCAATATA+TGG - chr4_4:34721858-34721877 None:intergenic 20.0%
!!! CATATATTGATTAAGAGAAA+AGG + chr4_4:34721856-34721875 Msa0678640:intron 20.0%
!! ATAACATCAATAACAAAGTT+TGG - chr4_4:34722033-34722052 None:intergenic 20.0%
!!! CACCAGTAATTATTTTTTAA+TGG + chr4_4:34722113-34722132 Msa0678640:intron 20.0%
! CTACAAATAGAGAAGAAATA+GGG - chr4_4:34721478-34721497 None:intergenic 25.0%
! ACTACAAATAGAGAAGAAAT+AGG - chr4_4:34721479-34721498 None:intergenic 25.0%
! TGAGTGGTGTATTAATATAT+AGG - chr4_4:34721555-34721574 None:intergenic 25.0%
!! GTATTTGATAATCAAGATGT+GGG - chr4_4:34721620-34721639 None:intergenic 25.0%
!! TGTATTTGATAATCAAGATG+TGG - chr4_4:34721621-34721640 None:intergenic 25.0%
!!! AACTTAATTTGCTATTGCAA+TGG + chr4_4:34721655-34721674 Msa0678640:exon 25.0%
!!! ATCTGTTGAAACATTTTTAG+AGG - chr4_4:34721696-34721715 None:intergenic 25.0%
!! AAAAGACATGCATTCTAAAA+GGG - chr4_4:34721729-34721748 None:intergenic 25.0%
! TAAAAGACATGCATTCTAAA+AGG - chr4_4:34721730-34721749 None:intergenic 25.0%
! AGAGAAAATGCTTATTACAA+TGG + chr4_4:34721936-34721955 Msa0678640:CDS 25.0%
! ACAATGGTTTATAATCTTGT+TGG + chr4_4:34721952-34721971 Msa0678640:CDS 25.0%
! CAATGGTTTATAATCTTGTT+GGG + chr4_4:34721953-34721972 Msa0678640:CDS 25.0%
! AATGGTTTATAATCTTGTTG+GGG + chr4_4:34721954-34721973 Msa0678640:CDS 25.0%
!! ATGAATGTGTATTTTCTCAT+AGG + chr4_4:34722280-34722299 Msa0678640:intron 25.0%
! CAGAAGAGATAGAAAAATAT+TGG + chr4_4:34722340-34722359 Msa0678640:CDS 25.0%
! TTTCTTGATTCATTCATAGT+TGG - chr4_4:34722397-34722416 None:intergenic 25.0%
! CTACAAATAGAGAAGAAATA+GGG - chr4_4:34721478-34721497 None:intergenic 25.0%
! ACTACAAATAGAGAAGAAAT+AGG - chr4_4:34721479-34721498 None:intergenic 25.0%
! TGAGTGGTGTATTAATATAT+AGG - chr4_4:34721555-34721574 None:intergenic 25.0%
!! GTATTTGATAATCAAGATGT+GGG - chr4_4:34721620-34721639 None:intergenic 25.0%
!! TGTATTTGATAATCAAGATG+TGG - chr4_4:34721621-34721640 None:intergenic 25.0%
!!! AACTTAATTTGCTATTGCAA+TGG + chr4_4:34721655-34721674 Msa0678640:exon 25.0%
!!! ATCTGTTGAAACATTTTTAG+AGG - chr4_4:34721696-34721715 None:intergenic 25.0%
!! AAAAGACATGCATTCTAAAA+GGG - chr4_4:34721729-34721748 None:intergenic 25.0%
! TAAAAGACATGCATTCTAAA+AGG - chr4_4:34721730-34721749 None:intergenic 25.0%
! AGAGAAAATGCTTATTACAA+TGG + chr4_4:34721936-34721955 Msa0678640:CDS 25.0%
! ACAATGGTTTATAATCTTGT+TGG + chr4_4:34721952-34721971 Msa0678640:CDS 25.0%
! CAATGGTTTATAATCTTGTT+GGG + chr4_4:34721953-34721972 Msa0678640:CDS 25.0%
! AATGGTTTATAATCTTGTTG+GGG + chr4_4:34721954-34721973 Msa0678640:CDS 25.0%
!! ATGAATGTGTATTTTCTCAT+AGG + chr4_4:34722280-34722299 Msa0678640:intron 25.0%
! CAGAAGAGATAGAAAAATAT+TGG + chr4_4:34722340-34722359 Msa0678640:CDS 25.0%
! TTTCTTGATTCATTCATAGT+TGG - chr4_4:34722397-34722416 None:intergenic 25.0%
!!! TGGTGTTATTTTGCAGAATA+TGG - chr4_4:34721591-34721610 None:intergenic 30.0%
AATGTTTCAACAGATTCCAA+AGG + chr4_4:34721701-34721720 Msa0678640:CDS 30.0%
AAAATTCAACCTTTGAACCT+TGG - chr4_4:34721909-34721928 None:intergenic 30.0%
! TTTGGAATGAAAGTGAAAAG+AGG - chr4_4:34722015-34722034 None:intergenic 30.0%
GTCTGATGTTTGTGTTTATT+TGG - chr4_4:34722069-34722088 None:intergenic 30.0%
! AATGTGTATTTTCTCATAGG+TGG + chr4_4:34722283-34722302 Msa0678640:intron 30.0%
!!! TGGTGTTATTTTGCAGAATA+TGG - chr4_4:34721591-34721610 None:intergenic 30.0%
AATGTTTCAACAGATTCCAA+AGG + chr4_4:34721701-34721720 Msa0678640:CDS 30.0%
AAAATTCAACCTTTGAACCT+TGG - chr4_4:34721909-34721928 None:intergenic 30.0%
! TTTGGAATGAAAGTGAAAAG+AGG - chr4_4:34722015-34722034 None:intergenic 30.0%
GTCTGATGTTTGTGTTTATT+TGG - chr4_4:34722069-34722088 None:intergenic 30.0%
! AATGTGTATTTTCTCATAGG+TGG + chr4_4:34722283-34722302 Msa0678640:intron 30.0%
TAGAGAAGAAATAGGGAAGA+CGG - chr4_4:34721471-34721490 None:intergenic 35.0%
TGGAATGATTGAGATTTGAG+TGG - chr4_4:34721571-34721590 None:intergenic 35.0%
AATCAAGATGTGGGAAACTA+TGG - chr4_4:34721611-34721630 None:intergenic 35.0%
TTTATAATCTTGTTGGGGAG+AGG + chr4_4:34721959-34721978 Msa0678640:CDS 35.0%
AAACATCAGACACGAAAGTA+AGG + chr4_4:34722078-34722097 Msa0678640:intron 35.0%
! AAACTTTCGATCTAGAGTGT+CGG + chr4_4:34722209-34722228 Msa0678640:intron 35.0%
TAGAGAAGAAATAGGGAAGA+CGG - chr4_4:34721471-34721490 None:intergenic 35.0%
TGGAATGATTGAGATTTGAG+TGG - chr4_4:34721571-34721590 None:intergenic 35.0%
AATCAAGATGTGGGAAACTA+TGG - chr4_4:34721611-34721630 None:intergenic 35.0%
TTTATAATCTTGTTGGGGAG+AGG + chr4_4:34721959-34721978 Msa0678640:CDS 35.0%
AAACATCAGACACGAAAGTA+AGG + chr4_4:34722078-34722097 Msa0678640:intron 35.0%
! AAACTTTCGATCTAGAGTGT+CGG + chr4_4:34722209-34722228 Msa0678640:intron 35.0%
AGAGAAGAAATAGGGAAGAC+GGG - chr4_4:34721470-34721489 None:intergenic 40.0%
!! GCATTCTAAAAGGGTACCTT+TGG - chr4_4:34721720-34721739 None:intergenic 40.0%
CTCTAGATCGAAAGTTTGTC+TGG - chr4_4:34722206-34722225 None:intergenic 40.0%
TTGATTGCTGGAAGAATTCC+TGG + chr4_4:34722309-34722328 Msa0678640:CDS 40.0%
AGAGAAGAAATAGGGAAGAC+GGG - chr4_4:34721470-34721489 None:intergenic 40.0%
!! GCATTCTAAAAGGGTACCTT+TGG - chr4_4:34721720-34721739 None:intergenic 40.0%
CTCTAGATCGAAAGTTTGTC+TGG - chr4_4:34722206-34722225 None:intergenic 40.0%
TTGATTGCTGGAAGAATTCC+TGG + chr4_4:34722309-34722328 Msa0678640:CDS 40.0%
GAGAAGAAATAGGGAAGACG+GGG - chr4_4:34721469-34721488 None:intergenic 45.0%
TCAACCTTTGAACCTTGGTC+TGG - chr4_4:34721904-34721923 None:intergenic 45.0%
TAATCTTGTTGGGGAGAGGT+TGG + chr4_4:34721963-34721982 Msa0678640:intron 45.0%
! CATAGGTGGTCATTGATTGC+TGG + chr4_4:34722297-34722316 Msa0678640:intron 45.0%
ATCTCTTCTGCTGTTCTTCC+AGG - chr4_4:34722330-34722349 None:intergenic 45.0%
GAGAAGAAATAGGGAAGACG+GGG - chr4_4:34721469-34721488 None:intergenic 45.0%
TCAACCTTTGAACCTTGGTC+TGG - chr4_4:34721904-34721923 None:intergenic 45.0%
TAATCTTGTTGGGGAGAGGT+TGG + chr4_4:34721963-34721982 Msa0678640:intron 45.0%
! CATAGGTGGTCATTGATTGC+TGG + chr4_4:34722297-34722316 Msa0678640:intron 45.0%
ATCTCTTCTGCTGTTCTTCC+AGG - chr4_4:34722330-34722349 None:intergenic 45.0%
ACAGATCAGCAACCAGACCA+AGG + chr4_4:34721889-34721908 Msa0678640:intron 50.0%
GCAACCAGACCAAGGTTCAA+AGG + chr4_4:34721897-34721916 Msa0678640:CDS 50.0%
ACAGATCAGCAACCAGACCA+AGG + chr4_4:34721889-34721908 Msa0678640:intron 50.0%
GCAACCAGACCAAGGTTCAA+AGG + chr4_4:34721897-34721916 Msa0678640:CDS 50.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 34721457 34722420 34721457 ID=Msa0678640;Name=Msa0678640
chr4_4 mRNA 34721457 34722420 34721457 ID=Msa0678640-mRNA-1;Parent=Msa0678640;Name=Msa0678640-mRNA-1;_AED=0.02;_eAED=0.02;_QI=217|1|1|1|1|1|3|31|74
chr4_4 exon 34721457 34721722 34721457 ID=Msa0678640-mRNA-1:exon:8322;Parent=Msa0678640-mRNA-1
chr4_4 exon 34721893 34721980 34721893 ID=Msa0678640-mRNA-1:exon:8323;Parent=Msa0678640-mRNA-1
chr4_4 exon 34722302 34722420 34722302 ID=Msa0678640-mRNA-1:exon:8324;Parent=Msa0678640-mRNA-1
chr4_4 five_prime_UTR 34721457 34721673 34721457 ID=Msa0678640-mRNA-1:five_prime_utr;Parent=Msa0678640-mRNA-1
chr4_4 CDS 34721674 34721722 34721674 ID=Msa0678640-mRNA-1:cds;Parent=Msa0678640-mRNA-1
chr4_4 CDS 34721893 34721980 34721893 ID=Msa0678640-mRNA-1:cds;Parent=Msa0678640-mRNA-1
chr4_4 CDS 34722302 34722389 34722302 ID=Msa0678640-mRNA-1:cds;Parent=Msa0678640-mRNA-1
chr4_4 three_prime_UTR 34722390 34722420 34722390 ID=Msa0678640-mRNA-1:three_prime_utr;Parent=Msa0678640-mRNA-1
Gene Sequence

>Msa0678640

ATGGCTGACTCAGAACACTCCTCTAAAAATGTTTCAACAGATTCCAAAGATCAGCAACCAGACCAAGGTTCAAAGGTTGAATTTTCTGAAGAAGAGAAAATGCTTATTACAATGGTTTATAATCTTGTTGGGGAGAGGTGGTCATTGATTGCTGGAAGAATTCCTGGAAGAACAGCAGAAGAGATAGAAAAATATTGGAATTCAAGACACTCGACTAGCAAATGA

Protein sequence

>Msa0678640

MADSEHSSKNVSTDSKDQQPDQGSKVEFSEEEKMLITMVYNLVGERWSLIAGRIPGRTAEEIEKYWNSRHSTSK*