AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0678880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0678880 MtrunA17_Chr4g0025061 67.537 613 115 8 8 553 16 611 0.0 707
Msa0678880 MtrunA17_Chr4g0024391 79.957 469 73 1 106 553 20 488 0.0 707
Msa0678880 MtrunA17_Chr4g0025051 70.893 560 103 3 25 524 8 567 0.0 684
Msa0678880 MtrunA17_Chr2g0322391 37.705 549 206 19 63 551 118 590 1.47e-75 250
Msa0678880 MtrunA17_Chr2g0288871 34.190 506 239 16 52 505 67 530 4.49e-62 213
Msa0678880 MtrunA17_Chr4g0024401 80.952 126 18 2 1 126 1 120 7.74e-59 191
Msa0678880 MtrunA17_Chr4g0075201 34.086 487 212 19 52 492 41 464 1.59e-54 192
Msa0678880 MtrunA17_Chr3g0099641 34.595 370 162 10 161 504 129 444 9.79e-50 178
Msa0678880 MtrunA17_Chr7g0270831 42.544 228 83 7 300 505 201 402 3.42e-44 162
Msa0678880 MtrunA17_Chr2g0302391 35.656 244 107 5 294 507 190 413 4.73e-36 139
Msa0678880 MtrunA17_Chr7g0216891 48.387 124 58 1 272 389 170 293 9.24e-31 126
Msa0678880 MtrunA17_Chr4g0051181 43.258 178 81 5 264 427 193 364 2.70e-29 121
Msa0678880 MtrunA17_Chr4g0060271 60.526 76 30 0 300 375 240 315 1.97e-26 113
Msa0678880 MtrunA17_Chr5g0419241 67.925 53 17 0 293 345 55 107 1.01e-20 87.4
Msa0678880 MtrunA17_Chr7g0218081 43.396 106 49 2 272 377 377 471 1.64e-20 95.1
Msa0678880 MtrunA17_Chr4g0001361 28.571 210 112 5 292 501 139 310 1.40e-19 89.7
Msa0678880 MtrunA17_Chr5g0418191 43.299 97 50 2 285 377 286 381 1.40e-19 91.7
Msa0678880 MtrunA17_Chr4g0042831 46.914 81 42 1 297 377 260 339 1.51e-18 87.8
Msa0678880 MtrunA17_Chr3g0079691 45.977 87 46 1 291 377 471 556 4.00e-18 88.2
Msa0678880 MtrunA17_Chr3g0101131 36.607 112 67 2 285 393 385 495 9.25e-18 86.7
Msa0678880 MtrunA17_Chr5g0445311 47.500 80 41 1 298 377 347 425 1.85e-17 85.5
Msa0678880 MtrunA17_Chr7g0249311 37.600 125 73 3 298 419 375 497 1.94e-17 85.9
Msa0678880 MtrunA17_Chr7g0243891 47.619 84 43 1 294 377 353 435 2.13e-17 85.5
Msa0678880 MtrunA17_Chr3g0106491 40.000 100 59 1 277 376 327 425 4.40e-17 84.3
Msa0678880 MtrunA17_Chr5g0432161 43.011 93 52 1 285 377 391 482 6.15e-17 84.0
Msa0678880 MtrunA17_Chr7g0249301 50.000 76 37 1 302 377 279 353 9.30e-17 82.4
Msa0678880 MtrunA17_Chr7g0270191 38.983 118 67 2 300 417 130 242 1.26e-16 80.9
Msa0678880 MtrunA17_Chr4g0004911 46.835 79 41 1 297 375 318 395 1.92e-16 82.0
Msa0678880 MtrunA17_Chr3g0136961 43.956 91 44 2 313 398 136 224 1.92e-16 80.1
Msa0678880 MtrunA17_Chr2g0333851 36.792 106 56 2 313 417 167 262 2.27e-16 80.5
Msa0678880 MtrunA17_Chr1g0150271 39.130 92 55 1 284 375 71 161 4.11e-16 78.6
Msa0678880 MtrunA17_Chr7g0270051 37.391 115 63 2 280 388 12 123 4.72e-16 78.6
Msa0678880 MtrunA17_Chr8g0379461 47.143 70 37 0 308 377 69 138 5.43e-16 77.8
Msa0678880 MtrunA17_Chr7g0263531 43.902 82 45 1 294 375 340 420 7.48e-16 80.1
Msa0678880 MtrunA17_Chr2g0293481 45.333 75 41 0 311 385 263 337 1.09e-15 79.7
Msa0678880 MtrunA17_Chr5g0447701 40.217 92 54 1 286 377 223 313 1.32e-15 79.0
Msa0678880 MtrunA17_Chr2g0284321 50.000 64 32 0 312 375 32 95 1.77e-15 75.9
Msa0678880 MtrunA17_Chr4g0051881 38.889 108 55 4 302 409 165 261 2.29e-15 77.8
Msa0678880 MtrunA17_Chr2g0319961 52.459 61 29 0 315 375 80 140 3.47e-15 75.9
Msa0678880 MtrunA17_Chr1g0150281 39.130 92 56 0 300 391 125 216 1.27e-14 74.7
Msa0678880 MtrunA17_Chr5g0443131 44.872 78 42 1 302 379 144 220 1.50e-14 74.7
Msa0678880 MtrunA17_Chr4g0068541 41.772 79 45 1 301 379 194 271 1.55e-14 75.9
Msa0678880 MtrunA17_Chr2g0294231 41.250 80 46 1 300 379 188 266 3.18e-14 74.7
Msa0678880 MtrunA17_Chr1g0202461 40.000 85 51 0 313 397 259 343 3.35e-14 74.7
Msa0678880 MtrunA17_Chr7g0248691 45.333 75 41 0 311 385 150 224 3.94e-14 73.6
Msa0678880 MtrunA17_Chr7g0224801 55.000 60 26 1 313 372 91 149 4.16e-14 70.5
Msa0678880 MtrunA17_Chr1g0147881 47.761 67 35 0 316 382 263 329 4.90e-14 73.6
Msa0678880 MtrunA17_Chr8g0387501 44.444 72 40 0 314 385 128 199 5.28e-14 72.4
Msa0678880 MtrunA17_Chr1g0188941 43.243 74 41 1 302 375 38 110 6.08e-14 68.6
Msa0678880 MtrunA17_Chr1g0194481 38.636 88 47 2 314 401 157 237 2.00e-13 71.6
Msa0678880 MtrunA17_Chr2g0289181 38.961 77 47 0 310 386 9 85 3.77e-13 69.3
Msa0678880 MtrunA17_Chr8g0348311 42.667 75 43 0 311 385 151 225 7.38e-13 70.1
Msa0678880 MtrunA17_Chr8g0386241 32.710 107 71 1 273 379 61 166 7.58e-13 67.4
Msa0678880 MtrunA17_Chr4g0053281 48.387 62 32 0 316 377 267 328 9.43e-13 69.7
Msa0678880 MtrunA17_Chr5g0418781 41.772 79 45 1 294 372 103 180 1.45e-12 68.2
Msa0678880 MtrunA17_Chr1g0184691 49.123 57 29 0 316 372 294 350 2.04e-12 68.9
Msa0678880 MtrunA17_Chr6g0485431 35.897 117 69 3 302 413 141 256 2.31e-12 68.6
Msa0678880 MtrunA17_Chr1g0150801 42.466 73 41 1 300 372 87 158 2.53e-12 65.1
Msa0678880 MtrunA17_Chr7g0248721 53.333 60 26 2 313 372 139 196 3.19e-12 66.2
Msa0678880 MtrunA17_Chr4g0074001 42.500 80 45 1 300 379 344 422 6.08e-12 68.2
Msa0678880 MtrunA17_Chr5g0401821 48.333 60 31 0 315 374 199 258 7.06e-12 66.2
Msa0678880 MtrunA17_Chr8g0383251 38.961 77 46 1 302 378 95 170 3.08e-11 62.8
Msa0678880 MtrunA17_Chr3g0129321 41.333 75 43 1 302 376 105 178 3.91e-11 62.8
Msa0678880 MtrunA17_Chr4g0071521 44.828 58 32 0 315 372 245 302 9.41e-11 63.2
Msa0678880 MtrunA17_Chr7g0228941 43.860 57 32 0 316 372 53 109 9.92e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0678880 AT4G04450.1 35.503 507 240 13 52 504 30 503 6.89e-64 218
Msa0678880 AT4G04450.2 41.714 350 140 8 195 504 84 409 2.40e-59 203
Msa0678880 AT1G62300.1 42.857 266 107 6 294 518 291 552 3.87e-50 181
Msa0678880 AT4G22070.4 46.694 242 95 4 294 504 276 514 1.70e-49 179
Msa0678880 AT4G22070.3 46.694 242 95 4 294 504 276 514 1.70e-49 179
Msa0678880 AT4G22070.2 46.694 242 95 4 294 504 276 514 1.70e-49 179
Msa0678880 AT4G22070.1 46.694 242 95 4 294 504 276 514 1.70e-49 179
Msa0678880 AT4G01720.1 43.515 239 79 6 300 501 224 443 4.58e-36 141
Msa0678880 AT1G68150.1 55.446 101 45 0 300 400 220 320 4.37e-32 127
Msa0678880 AT5G15130.2 58.696 92 38 0 298 389 168 259 1.82e-31 128
Msa0678880 AT5G15130.1 58.696 92 38 0 298 389 210 301 2.48e-31 128
Msa0678880 AT1G69810.1 42.038 157 69 4 292 429 180 333 9.59e-28 115
Msa0678880 AT1G69810.2 42.038 157 69 4 292 429 122 275 1.03e-27 114
Msa0678880 AT1G18860.1 57.292 96 41 0 298 393 222 317 3.51e-26 112
Msa0678880 AT2G03340.1 36.364 143 71 3 235 377 354 476 1.36e-19 92.4
Msa0678880 AT1G13960.1 49.367 79 39 1 297 375 391 468 6.23e-19 90.5
Msa0678880 AT1G13960.2 49.367 79 39 1 297 375 238 315 9.95e-19 88.6
Msa0678880 AT2G25000.3 29.101 189 121 3 202 378 1 188 1.15e-17 83.2
Msa0678880 AT2G38470.1 41.284 109 58 2 297 405 344 446 1.31e-17 86.3
Msa0678880 AT4G26640.5 45.000 80 43 1 298 377 188 266 1.59e-17 85.1
Msa0678880 AT4G26640.6 45.000 80 43 1 298 377 188 266 1.59e-17 85.1
Msa0678880 AT4G26640.4 45.000 80 43 1 298 377 188 266 1.59e-17 85.1
Msa0678880 AT4G26640.3 45.000 80 43 1 298 377 188 266 1.59e-17 85.1
Msa0678880 AT4G26640.7 45.000 80 43 1 298 377 324 402 1.99e-17 85.9
Msa0678880 AT4G26640.1 45.000 80 43 1 298 377 292 370 2.22e-17 85.5
Msa0678880 AT2G25000.2 41.573 89 52 0 290 378 78 166 2.53e-17 81.6
Msa0678880 AT2G25000.4 41.573 89 52 0 290 378 78 166 2.53e-17 81.6
Msa0678880 AT4G26640.2 45.000 80 43 1 298 377 364 442 2.58e-17 85.5
Msa0678880 AT5G56270.1 47.500 80 41 1 298 377 470 548 2.60e-17 85.9
Msa0678880 AT2G25000.1 41.573 89 52 0 290 378 121 209 3.35e-17 82.4
Msa0678880 AT3G01080.2 43.023 86 48 1 290 375 319 403 4.30e-17 84.3
Msa0678880 AT3G01080.1 43.529 85 47 1 291 375 282 365 5.96e-17 83.6
Msa0678880 AT5G46350.1 42.857 98 54 2 302 399 170 265 1.20e-16 81.6
Msa0678880 AT4G31800.2 42.500 80 46 0 314 393 110 189 2.34e-16 79.3
Msa0678880 AT1G80840.1 48.438 64 33 0 313 376 144 207 2.56e-16 80.5
Msa0678880 AT4G31800.1 42.500 80 46 0 314 393 175 254 3.78e-16 80.1
Msa0678880 AT4G23550.1 44.872 78 43 0 310 387 135 212 4.55e-16 79.7
Msa0678880 AT2G30250.1 43.902 82 45 1 298 379 311 391 9.52e-16 79.7
Msa0678880 AT1G29860.1 40.426 94 51 2 300 393 121 209 1.78e-15 77.4
Msa0678880 AT5G52830.1 35.156 128 77 2 304 430 154 276 2.18e-15 78.2
Msa0678880 AT5G07100.1 42.353 85 48 1 295 379 214 297 2.56e-15 77.4
Msa0678880 AT5G07100.2 42.353 85 48 1 295 379 121 204 2.69e-15 75.5
Msa0678880 AT5G07100.4 42.353 85 48 1 295 379 121 204 2.69e-15 75.5
Msa0678880 AT5G07100.5 42.353 85 48 1 295 379 121 204 2.69e-15 75.5
Msa0678880 AT5G07100.3 42.353 85 48 1 295 379 121 204 2.69e-15 75.5
Msa0678880 AT4G18170.1 47.368 76 39 1 300 375 157 231 3.68e-15 77.0
Msa0678880 AT1G64000.1 45.783 83 44 1 290 372 89 170 5.09e-15 74.3
Msa0678880 AT1G29280.1 49.180 61 31 0 315 375 74 134 1.00e-14 74.7
Msa0678880 AT2G34830.1 45.833 72 39 0 311 382 211 282 1.02e-14 77.0
Msa0678880 AT4G26440.1 46.053 76 40 1 302 377 359 433 1.09e-14 77.4
Msa0678880 AT5G41570.1 48.611 72 36 1 301 372 84 154 1.19e-14 72.8
Msa0678880 AT2G34830.2 45.833 72 39 0 311 382 220 291 1.30e-14 76.6
Msa0678880 AT1G30650.1 45.833 72 39 0 311 382 213 284 1.36e-14 76.6
Msa0678880 AT2G04880.2 37.363 91 56 1 285 375 253 342 1.37e-14 76.6
Msa0678880 AT2G04880.1 37.363 91 56 1 285 375 277 366 1.69e-14 76.6
Msa0678880 AT2G47260.1 40.964 83 48 1 300 382 159 240 2.04e-14 75.1
Msa0678880 AT5G49520.1 46.970 66 34 1 314 379 220 284 2.24e-14 75.5
Msa0678880 AT4G01250.1 45.333 75 41 0 311 385 124 198 3.55e-14 73.9
Msa0678880 AT3G58710.1 46.875 64 34 0 315 378 71 134 4.03e-14 73.2
Msa0678880 AT3G58710.2 46.875 64 34 0 315 378 70 133 4.17e-14 73.2
Msa0678880 AT2G46130.1 42.308 78 44 1 295 372 10 86 8.52e-14 68.2
Msa0678880 AT5G13080.1 55.000 60 26 1 313 372 65 123 9.15e-14 69.3
Msa0678880 AT5G26170.1 43.056 72 40 1 301 372 99 169 2.71e-13 68.6
Msa0678880 AT3G01970.1 47.222 72 37 1 301 372 51 121 2.88e-13 67.8
Msa0678880 AT4G30935.1 36.607 112 65 2 300 406 316 426 1.71e-12 70.1
Msa0678880 AT2G30590.1 48.438 64 32 1 316 378 314 377 1.95e-12 69.3
Msa0678880 AT1G55600.1 35.238 105 67 1 290 394 282 385 2.26e-12 69.7
Msa0678880 AT5G45050.4 35.965 114 57 2 315 412 1041 1154 2.59e-12 70.5
Msa0678880 AT5G45050.1 35.965 114 57 2 315 412 1180 1293 2.69e-12 70.5
Msa0678880 AT5G45050.2 35.965 114 57 2 315 412 1152 1265 2.72e-12 70.5
Msa0678880 AT2G23320.1 47.458 59 31 0 316 374 241 299 2.90e-12 68.6
Msa0678880 AT1G69310.4 39.241 79 47 1 294 372 126 203 5.22e-12 67.4
Msa0678880 AT1G69310.2 39.241 79 47 1 294 372 126 203 5.22e-12 67.4
Msa0678880 AT1G69310.1 39.241 79 47 1 294 372 126 203 5.22e-12 67.4
Msa0678880 AT4G24240.1 46.667 60 32 0 315 374 281 340 8.37e-12 67.4
Msa0678880 AT2G24570.1 49.123 57 29 0 316 372 244 300 1.10e-11 66.6
Msa0678880 AT3G62340.1 37.778 90 50 2 310 394 113 201 1.39e-11 65.9
Msa0678880 AT5G28650.1 49.057 53 27 0 320 372 267 319 1.43e-11 66.2
Msa0678880 AT2G37260.3 40.404 99 40 3 309 407 263 342 2.06e-11 66.2
Msa0678880 AT2G37260.2 40.404 99 40 3 309 407 261 340 2.11e-11 65.9
Msa0678880 AT2G37260.1 48.485 66 33 1 309 374 343 407 2.36e-11 66.2
Msa0678880 AT3G04670.1 47.368 57 30 0 316 372 263 319 2.47e-11 65.9
Msa0678880 AT2G44745.1 40.789 76 44 1 298 373 128 202 2.99e-11 63.9

Find 95 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAATCTGCAGGAAAATT+TGG 0.148510 4_4:-35686031 None:intergenic
GAAGTTTCTGATGGAAAATT+TGG 0.155377 4_4:+35684332 Msa0678880:CDS
CCTAACTGATCCTTACTTCT+AGG 0.157547 4_4:-35684581 None:intergenic
TTAACTATCTGAACAATATT+TGG 0.195632 4_4:-35685989 None:intergenic
TCTAAAATTGGCTAGTAATA+AGG 0.203492 4_4:+35684289 Msa0678880:CDS
AATAGAGTTAGATTTCTTTA+AGG 0.203537 4_4:+35682440 Msa0678880:CDS
GAAATTTATGGATCTAAAAT+TGG 0.207024 4_4:+35684277 Msa0678880:CDS
GCAGAACTCTGAGTGATTAA+AGG 0.218319 4_4:-35685857 None:intergenic
GCTTCTCTTACTTCCATTAT+TGG 0.227500 4_4:+35686055 Msa0678880:CDS
TCACGTGTAATTGTTGTAAA+TGG 0.235076 4_4:-35685782 None:intergenic
GGGTTCCTTTGTGGAGTATT+TGG 0.246074 4_4:-35685818 None:intergenic
GTATTTGATGGAAACTTTAA+AGG 0.252144 4_4:+35684215 Msa0678880:CDS
CTTTAACTTCCTTAACATTA+TGG 0.256499 4_4:-35684184 None:intergenic
ACTAGGGCCAATGAAATTTA+TGG 0.259833 4_4:+35684265 Msa0678880:CDS
CTTTGGCTGCTGCAGGTAAC+AGG 0.303664 4_4:-35685679 None:intergenic
AATAAGGGTAAGGAAGTTAA+AGG 0.305666 4_4:+35684305 Msa0678880:CDS
AAGGTTAAAGAAGTGTTTAA+TGG 0.314983 4_4:+35684428 Msa0678880:CDS
TGCTGGATTGGATACTAATA+AGG 0.317920 4_4:+35684409 Msa0678880:CDS
CATAATGTTAAGGAAGTTAA+AGG 0.325037 4_4:+35684185 Msa0678880:CDS
TACTGATACACCATCTTTGT+TGG 0.335785 4_4:+35682524 Msa0678880:CDS
TTGCAGCGATAATAAGCTTT+AGG 0.341456 4_4:-35684900 None:intergenic
AAAGGAGTTTGAAACTGATC+TGG 0.348918 4_4:-35685839 None:intergenic
AACTGATCTGGGTTCCTTTG+TGG 0.354361 4_4:-35685827 None:intergenic
GCTACCATTGCTATTTCCTT+TGG 0.355410 4_4:-35686131 None:intergenic
CAAATCTCATCTCATGAAAC+AGG 0.365816 4_4:+35682417 Msa0678880:CDS
AAAAGGAAATTTGTTGATGC+TGG 0.371075 4_4:+35684392 Msa0678880:CDS
GCCATTTCTTTGGCTGCTGC+AGG 0.373631 4_4:-35685686 None:intergenic
ACTGAGAATGGTGGAGTACT+AGG 0.377885 4_4:+35684248 Msa0678880:CDS
TAGCTGAGTATCACCAATAA+TGG 0.383371 4_4:-35686068 None:intergenic
TTTAGATCCATAAATTTCAT+TGG 0.385662 4_4:-35684272 None:intergenic
AGCTGGCCCTTCACAACTAT+TGG 0.390929 4_4:+35685961 Msa0678880:CDS
CTAAAATTGGCTAGTAATAA+GGG 0.398124 4_4:+35684290 Msa0678880:CDS
TGCTATTTCCTTTGGTGACA+TGG 0.403522 4_4:-35686123 None:intergenic
AAGCATCGAGGTTGCTTTGA+AGG 0.415231 4_4:+35684616 Msa0678880:CDS
AAAGGAAAAGAAGTATTTGA+TGG 0.422383 4_4:+35684203 Msa0678880:CDS
GAAATTTGTTGATGCTGGAT+TGG 0.422880 4_4:+35684397 Msa0678880:CDS
TGGTTCTGATTAGGTATTTG+AGG 0.425279 4_4:-35685902 None:intergenic
TATTTGATGGAAACTTTAAA+GGG 0.425379 4_4:+35684216 Msa0678880:CDS
GTCGCAGAAGACAATAGAAA+TGG 0.432622 4_4:+35686163 Msa0678880:CDS
GATGCAAGACCATAATGTTA+AGG 0.441157 4_4:+35684175 Msa0678880:CDS
ACCTGCAGCAGCCAAAGAAA+TGG 0.462652 4_4:+35685685 Msa0678880:CDS
GGTTCTGATTAGGTATTTGA+GGG 0.462686 4_4:-35685901 None:intergenic
CCTAGAAGTAAGGATCAGTT+AGG 0.469639 4_4:+35684581 Msa0678880:CDS
GCAAGAGTTACTATTAGAGC+TGG 0.472554 4_4:+35684701 Msa0678880:CDS
TAATAACATTACCTCCCTCT+TGG 0.475643 4_4:-35682817 None:intergenic
ATGATCATTGTCAGTGGCAG+AGG 0.475820 4_4:-35682496 None:intergenic
AGTATTGGTAGTAACATGAT+TGG 0.476430 4_4:-35682736 None:intergenic
TAGTAGTGATCAATCATTGA+TGG 0.477983 4_4:+35682758 Msa0678880:CDS
TGGTTGCTCAGTCCGAAAAC+AGG 0.478137 4_4:+35684932 Msa0678880:CDS
ACATGTAGCAACAACAATGA+TGG 0.479972 4_4:+35686193 Msa0678880:CDS
GATGAGAAAACCTAGAAGTA+AGG 0.486100 4_4:+35684571 Msa0678880:CDS
CGATAATAAGCTTTAGGACA+TGG 0.492862 4_4:-35684894 None:intergenic
GAGTTAATGATGACTTGGAA+AGG 0.508115 4_4:-35682372 None:intergenic
ATGATGGAGAATTAGTGAAA+AGG 0.514995 4_4:+35684375 Msa0678880:CDS
AGAGAAGCTAACAAATCTGC+AGG 0.517768 4_4:-35686040 None:intergenic
AAGGAGTTTGAAACTGATCT+GGG 0.518182 4_4:-35685838 None:intergenic
CTGCAGGTAACAGGTGGTTG+TGG 0.520577 4_4:-35685670 None:intergenic
GTTGTAAATGGAGCAGAAGC+TGG 0.523998 4_4:-35685770 None:intergenic
ATTGGCTAGTAATAAGGGTA+AGG 0.524598 4_4:+35684295 Msa0678880:CDS
TGGAGGAGTTAATGATGACT+TGG 0.535839 4_4:-35682377 None:intergenic
AGATCACTGATGGATGTCAA+TGG 0.538244 4_4:+35684838 Msa0678880:intron
GGTATTGCCAATAGTTGTGA+AGG 0.538900 4_4:-35685968 None:intergenic
TGATTGATCACTACTAGTAT+TGG 0.547112 4_4:-35682751 None:intergenic
GATAATAAGCTTTAGGACAT+GGG 0.551511 4_4:-35684893 None:intergenic
AGTTAATGATGACTTGGAAA+GGG 0.551831 4_4:-35682371 None:intergenic
GATCAATCATTGATGGATGA+TGG 0.555481 4_4:+35682765 Msa0678880:CDS
AAAGGACAAGAAGTTTCTGA+TGG 0.557815 4_4:+35684323 Msa0678880:CDS
CATGTAGCAACAACAATGAT+GGG 0.564604 4_4:+35686194 Msa0678880:CDS
AATTGAGAATGGTGGAGAGT+TGG 0.564860 4_4:+35684472 Msa0678880:CDS
AGCTTGAGTTCCAACAAAGA+TGG 0.564900 4_4:-35682534 None:intergenic
AAAATTTGGATTAGCAGCAA+TGG 0.568779 4_4:-35686017 None:intergenic
ACTTAGTCCCAACGAAAACA+TGG 0.569432 4_4:+35682329 Msa0678880:CDS
CTGAGAATGGTGGAGTACTA+GGG 0.577791 4_4:+35684249 Msa0678880:CDS
GTATTGGTAGTAACATGATT+GGG 0.578993 4_4:-35682735 None:intergenic
TTGAGGGATTATAGAAGCTG+AGG 0.585116 4_4:-35685885 None:intergenic
TATCGCTGCAACACATCCCA+TGG 0.592347 4_4:+35684912 Msa0678880:CDS
TACTAATGCTGAGACTGCGA+TGG 0.595069 4_4:+35684526 Msa0678880:CDS
ATGGTGGAGAGTTGGTGCAA+AGG 0.595504 4_4:+35684480 Msa0678880:CDS
GTATTGCCAATAGTTGTGAA+GGG 0.598828 4_4:-35685967 None:intergenic
AACGACAACCATGTCACCAA+AGG 0.600496 4_4:+35686115 Msa0678880:CDS
GTCACCAAAGGAAATAGCAA+TGG 0.610827 4_4:+35686127 Msa0678880:CDS
TGGCTGCTGCAGGTAACAGG+TGG 0.615814 4_4:-35685676 None:intergenic
TTCCAACTTAACAGGTACAA+AGG 0.616787 4_4:+35685603 Msa0678880:intron
AAGTAAGGATCAGTTAGGAT+CGG 0.620379 4_4:+35684586 Msa0678880:CDS
TGCGGACAGAAATTGTCGAA+AGG 0.625720 4_4:+35684867 Msa0678880:CDS
ATGAATATGATCATTGTCAG+TGG 0.630171 4_4:-35682502 None:intergenic
AGGCAAAGACAACTTCATGA+TGG 0.639024 4_4:-35682476 None:intergenic
CAAATGTCAAACGATGCAGC+TGG 0.639070 4_4:+35685944 Msa0678880:CDS
AGCTGGATTAGAAGAAACCA+TGG 0.639719 4_4:+35684718 Msa0678880:CDS
CAATACTTGAAGAACAAGGA+AGG 0.640614 4_4:-35685739 None:intergenic
TTCATGAGATGAGATTTGAG+AGG 0.652871 4_4:-35682412 None:intergenic
AATCTCCAAATACTCCACAA+AGG 0.678231 4_4:+35685813 Msa0678880:CDS
GTTGCAATACTTGAAGAACA+AGG 0.694121 4_4:-35685743 None:intergenic
GGATGTCAATGGAGAAAGTG+CGG 0.714843 4_4:+35684849 Msa0678880:CDS
ATCGGCGATGAAAAGCATCG+AGG 0.778036 4_4:+35684604 Msa0678880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAGTAAAAAAAACTATAA+TGG - chr4_4:35682587-35682606 None:intergenic 10.0%
!!! AACTTTATTTTTCTTTTATT+TGG + chr4_4:35683515-35683534 Msa0678880:intron 10.0%
!! TGAATAAATAAATTCAATTT+AGG - chr4_4:35683578-35683597 None:intergenic 10.0%
!!! TTGTTTTTTCAAATTATTAT+TGG + chr4_4:35683688-35683707 Msa0678880:intron 10.0%
!!! TTTTTGTATTCTAAATTATG+TGG - chr4_4:35682795-35682814 None:intergenic 15.0%
!! TAATTTCATTAAATTTAGCT+AGG - chr4_4:35682846-35682865 None:intergenic 15.0%
!!! ATTTTTTCCTTTCATTTTAT+TGG + chr4_4:35682879-35682898 Msa0678880:intron 15.0%
!!! TTTTTTCCTTTCATTTTATT+GGG + chr4_4:35682880-35682899 Msa0678880:intron 15.0%
!! AGTAAGAGATAAAAAAATAT+AGG - chr4_4:35683113-35683132 None:intergenic 15.0%
!! GAAAACATAAATATTGTATA+TGG - chr4_4:35683214-35683233 None:intergenic 15.0%
!! TTTAAAAACATTAACTAATG+TGG + chr4_4:35683336-35683355 Msa0678880:intron 15.0%
!!! TAGAATATTTTTATGTGATT+TGG + chr4_4:35683794-35683813 Msa0678880:intron 15.0%
!! TAAATAAAATACGTGTAATT+TGG - chr4_4:35684978-35684997 None:intergenic 15.0%
!!! TACACGTATTTTATTTATTA+AGG + chr4_4:35684981-35685000 Msa0678880:intron 15.0%
!!! ATGCATATTTTGATTTTTAA+TGG - chr4_4:35685410-35685429 None:intergenic 15.0%
!! TAATTAAAATACTTATGAAG+TGG + chr4_4:35685547-35685566 Msa0678880:intron 15.0%
!! AATAGAGTTAGATTTCTTTA+AGG + chr4_4:35682440-35682459 Msa0678880:CDS 20.0%
!!! TATAATGGACTATTGAATTA+TGG - chr4_4:35682572-35682591 None:intergenic 20.0%
!! TTGAAATAAAGGGAAAAAAT+GGG - chr4_4:35683191-35683210 None:intergenic 20.0%
!! TTGTATATGGTTGAAATAAA+GGG - chr4_4:35683201-35683220 None:intergenic 20.0%
!! ATTGTATATGGTTGAAATAA+AGG - chr4_4:35683202-35683221 None:intergenic 20.0%
!! GTCATATTCATTAATTAACA+CGG - chr4_4:35683305-35683324 None:intergenic 20.0%
!! TCAATTAATAACGAGTTTAA+TGG - chr4_4:35683422-35683441 None:intergenic 20.0%
!! AAACAATAATACTTAAACAC+TGG - chr4_4:35683743-35683762 None:intergenic 20.0%
!!! TTAAGTATTATTGTTTTGTC+CGG + chr4_4:35683747-35683766 Msa0678880:intron 20.0%
!! TATTTGATGGAAACTTTAAA+GGG + chr4_4:35684216-35684235 Msa0678880:CDS 20.0%
!! TTTAGATCCATAAATTTCAT+TGG - chr4_4:35684275-35684294 None:intergenic 20.0%
!!! GAAATTTATGGATCTAAAAT+TGG + chr4_4:35684277-35684296 Msa0678880:CDS 20.0%
!! AAAAAGAGAATGAATAATGA+TGG + chr4_4:35684359-35684378 Msa0678880:CDS 20.0%
!! AAGATAATGATTAAAGACTA+TGG - chr4_4:35685175-35685194 None:intergenic 20.0%
!!! GTATTTTAATTAACCTATCT+TGG - chr4_4:35685539-35685558 None:intergenic 20.0%
!! TTAACTATCTGAACAATATT+TGG - chr4_4:35685992-35686011 None:intergenic 20.0%
!!! TTTTTTCTTCTTCAACAGAT+TGG + chr4_4:35682711-35682730 Msa0678880:intron 25.0%
! TAGAATACAAAAAAACCAAG+AGG + chr4_4:35682802-35682821 Msa0678880:CDS 25.0%
! AGAATACAAAAAAACCAAGA+GGG + chr4_4:35682803-35682822 Msa0678880:CDS 25.0%
!! TTTTGACCCAATAAAATGAA+AGG - chr4_4:35682889-35682908 None:intergenic 25.0%
! TCAACCATATATATGTTTGT+TGG + chr4_4:35683047-35683066 Msa0678880:intron 25.0%
! GTTGAAATAAAGGGAAAAAA+TGG - chr4_4:35683192-35683211 None:intergenic 25.0%
! TTAACACGGTAATATAACAT+TGG - chr4_4:35683291-35683310 None:intergenic 25.0%
! ATTAATAACGAGTTTAATGG+AGG - chr4_4:35683419-35683438 None:intergenic 25.0%
! TGTATGTATCAGTATCATAT+GGG - chr4_4:35683652-35683671 None:intergenic 25.0%
! TTGTATGTATCAGTATCATA+TGG - chr4_4:35683653-35683672 None:intergenic 25.0%
! ACTAACATATACATAAACAC+CGG - chr4_4:35683769-35683788 None:intergenic 25.0%
! TTAGAACACTATTAACATGT+TGG + chr4_4:35683888-35683907 Msa0678880:intron 25.0%
! TAGAACACTATTAACATGTT+GGG + chr4_4:35683889-35683908 Msa0678880:intron 25.0%
! CCAACAAAAACAAAATGTAT+TGG - chr4_4:35683917-35683936 None:intergenic 25.0%
!!! CCAATACATTTTGTTTTTGT+TGG + chr4_4:35683914-35683933 Msa0678880:intron 25.0%
! GATAATCGAATGCAATTTAA+TGG + chr4_4:35683936-35683955 Msa0678880:intron 25.0%
! AAACATCATCGCATAAAAAA+TGG - chr4_4:35684017-35684036 None:intergenic 25.0%
!! TTGATTGAATTGTTTTCAGA+TGG + chr4_4:35684040-35684059 Msa0678880:intron 25.0%
!! CTTTAACTTCCTTAACATTA+TGG - chr4_4:35684187-35684206 None:intergenic 25.0%
! CATAATGTTAAGGAAGTTAA+AGG + chr4_4:35684185-35684204 Msa0678880:CDS 25.0%
!!! AAAGGAAAAGAAGTATTTGA+TGG + chr4_4:35684203-35684222 Msa0678880:CDS 25.0%
! GTATTTGATGGAAACTTTAA+AGG + chr4_4:35684215-35684234 Msa0678880:CDS 25.0%
!! TCTAAAATTGGCTAGTAATA+AGG + chr4_4:35684289-35684308 Msa0678880:CDS 25.0%
! CTAAAATTGGCTAGTAATAA+GGG + chr4_4:35684290-35684309 Msa0678880:CDS 25.0%
!!! AAGGTTAAAGAAGTGTTTAA+TGG + chr4_4:35684428-35684447 Msa0678880:CDS 25.0%
! AAGAAAAAGAGAATTGAGAA+TGG + chr4_4:35684461-35684480 Msa0678880:CDS 25.0%
!!! GTTAGTTTTAAGTAAGAATG+CGG + chr4_4:35684640-35684659 Msa0678880:CDS 25.0%
!!! TTTTAATGTTGATAATGCTG+AGG + chr4_4:35684664-35684683 Msa0678880:CDS 25.0%
! ACAATTTATCATACTTACCA+TGG - chr4_4:35684738-35684757 None:intergenic 25.0%
! ATAAATTCTTTCAGCGAAAA+AGG - chr4_4:35684806-35684825 None:intergenic 25.0%
!!! TTATTGTTTTAGATCACTGA+TGG + chr4_4:35684828-35684847 Msa0678880:intron 25.0%
! TAATGTGAAAGCAATCATTA+GGG - chr4_4:35685023-35685042 None:intergenic 25.0%
! ATAATGTGAAAGCAATCATT+AGG - chr4_4:35685024-35685043 None:intergenic 25.0%
!! CATGTTTCTATTCTAGAAAA+TGG + chr4_4:35685328-35685347 Msa0678880:intron 25.0%
!! TCTATTCTAGAAAATGGATT+TGG + chr4_4:35685334-35685353 Msa0678880:intron 25.0%
! TCAAAATATGCATTGAAGTT+TGG + chr4_4:35685417-35685436 Msa0678880:intron 25.0%
! TAACAAACTTATATCCGAAA+TGG + chr4_4:35685507-35685526 Msa0678880:intron 25.0%
! TAATACAATTCCAACTTAAC+AGG + chr4_4:35685595-35685614 Msa0678880:intron 25.0%
!!! AATTTTGATTGGTTCTGATT+AGG - chr4_4:35685914-35685933 None:intergenic 25.0%
! AGTTAATGATGACTTGGAAA+GGG - chr4_4:35682374-35682393 None:intergenic 30.0%
!! TTGAGAGGTTTGATTTTCAT+TGG - chr4_4:35682400-35682419 None:intergenic 30.0%
ATGAATATGATCATTGTCAG+TGG - chr4_4:35682505-35682524 None:intergenic 30.0%
! GTATTGGTAGTAACATGATT+GGG - chr4_4:35682738-35682757 None:intergenic 30.0%
!! AGTATTGGTAGTAACATGAT+TGG - chr4_4:35682739-35682758 None:intergenic 30.0%
TGATTGATCACTACTAGTAT+TGG - chr4_4:35682754-35682773 None:intergenic 30.0%
TAGTAGTGATCAATCATTGA+TGG + chr4_4:35682758-35682777 Msa0678880:CDS 30.0%
CCAACCAACAAACATATATA+TGG - chr4_4:35683054-35683073 None:intergenic 30.0%
! CCATATATATGTTTGTTGGT+TGG + chr4_4:35683051-35683070 Msa0678880:intron 30.0%
AAATAAAGGGAAAAAATGGG+TGG - chr4_4:35683188-35683207 None:intergenic 30.0%
TTGATGTAGTAGCACATTAT+TGG + chr4_4:35683438-35683457 Msa0678880:intron 30.0%
AGAACACTATTAACATGTTG+GGG + chr4_4:35683890-35683909 Msa0678880:intron 30.0%
! CATCGCATAAAAAATGGATT+TGG - chr4_4:35684011-35684030 None:intergenic 30.0%
AATAAGGGTAAGGAAGTTAA+AGG + chr4_4:35684305-35684324 Msa0678880:CDS 30.0%
GAAGTTTCTGATGGAAAATT+TGG + chr4_4:35684332-35684351 Msa0678880:CDS 30.0%
! ATGATGGAGAATTAGTGAAA+AGG + chr4_4:35684375-35684394 Msa0678880:CDS 30.0%
!! AAAAGGAAATTTGTTGATGC+TGG + chr4_4:35684392-35684411 Msa0678880:CDS 30.0%
AAAAAGAGAATTGAGAATGG+TGG + chr4_4:35684464-35684483 Msa0678880:CDS 30.0%
!! GATAATAAGCTTTAGGACAT+GGG - chr4_4:35684896-35684915 None:intergenic 30.0%
! AGATGAAAGTTACCTGTTTT+CGG - chr4_4:35684947-35684966 None:intergenic 30.0%
TATCTTGGTATCTACCATTT+CGG - chr4_4:35685524-35685543 None:intergenic 30.0%
!!! AGTTGTTTTTGCCATTTCTT+TGG - chr4_4:35685699-35685718 None:intergenic 30.0%
TCACGTGTAATTGTTGTAAA+TGG - chr4_4:35685785-35685804 None:intergenic 30.0%
! CAGAACCAATCAAAATTTTC+TGG + chr4_4:35685917-35685936 Msa0678880:CDS 30.0%
!! GTAGTCCAGAAAATTTTGAT+TGG - chr4_4:35685925-35685944 None:intergenic 30.0%
!! AAAATTTGGATTAGCAGCAA+TGG - chr4_4:35686020-35686039 None:intergenic 30.0%
AACAAATCTGCAGGAAAATT+TGG - chr4_4:35686034-35686053 None:intergenic 30.0%
!!! TGGGACTAAGTTTTAACTCA+AGG - chr4_4:35682320-35682339 None:intergenic 35.0%
! GAGTTAATGATGACTTGGAA+AGG - chr4_4:35682375-35682394 None:intergenic 35.0%
!! AGAGGTTTGATTTTCATTGG+AGG - chr4_4:35682397-35682416 None:intergenic 35.0%
TTCATGAGATGAGATTTGAG+AGG - chr4_4:35682415-35682434 None:intergenic 35.0%
CAAATCTCATCTCATGAAAC+AGG + chr4_4:35682417-35682436 Msa0678880:CDS 35.0%
TACTGATACACCATCTTTGT+TGG + chr4_4:35682524-35682543 Msa0678880:CDS 35.0%
! GATCAATCATTGATGGATGA+TGG + chr4_4:35682765-35682784 Msa0678880:CDS 35.0%
ATACAAAAAAACCAAGAGGG+AGG + chr4_4:35682806-35682825 Msa0678880:CDS 35.0%
TAATAACATTACCTCCCTCT+TGG - chr4_4:35682820-35682839 None:intergenic 35.0%
TCGTTAGATATGAAGATGTC+CGG + chr4_4:35682938-35682957 Msa0678880:intron 35.0%
TACTCACATAGACAACAATG+TGG + chr4_4:35682993-35683012 Msa0678880:intron 35.0%
ACTCACATAGACAACAATGT+GGG + chr4_4:35682994-35683013 Msa0678880:intron 35.0%
! ATTGTGTGTGCAAATTTGCT+TGG + chr4_4:35683380-35683399 Msa0678880:intron 35.0%
! TTGTGTGTGCAAATTTGCTT+GGG + chr4_4:35683381-35683400 Msa0678880:intron 35.0%
ATAACGAGTTTAATGGAGGT+GGG - chr4_4:35683415-35683434 None:intergenic 35.0%
AATAACGAGTTTAATGGAGG+TGG - chr4_4:35683416-35683435 None:intergenic 35.0%
TTTCAGATGGCTATTCTTCA+AGG + chr4_4:35684053-35684072 Msa0678880:intron 35.0%
CGAGATTGAACGAAAGAAAA+TGG + chr4_4:35684076-35684095 Msa0678880:intron 35.0%
GAACAGATTACGATTACATG+CGG + chr4_4:35684135-35684154 Msa0678880:intron 35.0%
GATGCAAGACCATAATGTTA+AGG + chr4_4:35684175-35684194 Msa0678880:CDS 35.0%
AAAAAGAGAACTGAGAATGG+TGG + chr4_4:35684239-35684258 Msa0678880:CDS 35.0%
ACTAGGGCCAATGAAATTTA+TGG + chr4_4:35684265-35684284 Msa0678880:CDS 35.0%
ATTGGCTAGTAATAAGGGTA+AGG + chr4_4:35684295-35684314 Msa0678880:CDS 35.0%
AAAGGACAAGAAGTTTCTGA+TGG + chr4_4:35684323-35684342 Msa0678880:CDS 35.0%
!! GAAATTTGTTGATGCTGGAT+TGG + chr4_4:35684397-35684416 Msa0678880:CDS 35.0%
! TGCTGGATTGGATACTAATA+AGG + chr4_4:35684409-35684428 Msa0678880:CDS 35.0%
GATGAGAAAACCTAGAAGTA+AGG + chr4_4:35684571-35684590 Msa0678880:CDS 35.0%
AAGTAAGGATCAGTTAGGAT+CGG + chr4_4:35684586-35684605 Msa0678880:CDS 35.0%
!! CGATAATAAGCTTTAGGACA+TGG - chr4_4:35684897-35684916 None:intergenic 35.0%
TTGCAGCGATAATAAGCTTT+AGG - chr4_4:35684903-35684922 None:intergenic 35.0%
TCAGAGAGTTTAATGTCGTT+GGG - chr4_4:35685111-35685130 None:intergenic 35.0%
ATCAGAGAGTTTAATGTCGT+TGG - chr4_4:35685112-35685131 None:intergenic 35.0%
TGATATGCATTGAACGATGT+TGG + chr4_4:35685261-35685280 Msa0678880:intron 35.0%
ATGGTTTCTGTATCGAAGAT+TGG - chr4_4:35685448-35685467 None:intergenic 35.0%
TCACATAGTAAGTACATCGA+TGG - chr4_4:35685467-35685486 None:intergenic 35.0%
GTACTTACTATGTGAATGCA+TGG + chr4_4:35685472-35685491 Msa0678880:intron 35.0%
GAAATGGTAGATACCAAGAT+AGG + chr4_4:35685523-35685542 Msa0678880:intron 35.0%
TTCCAACTTAACAGGTACAA+AGG + chr4_4:35685603-35685622 Msa0678880:intron 35.0%
CAATACTTGAAGAACAAGGA+AGG - chr4_4:35685742-35685761 None:intergenic 35.0%
GTTGCAATACTTGAAGAACA+AGG - chr4_4:35685746-35685765 None:intergenic 35.0%
AATCTCCAAATACTCCACAA+AGG + chr4_4:35685813-35685832 Msa0678880:CDS 35.0%
! AAGGAGTTTGAAACTGATCT+GGG - chr4_4:35685841-35685860 None:intergenic 35.0%
! AAAGGAGTTTGAAACTGATC+TGG - chr4_4:35685842-35685861 None:intergenic 35.0%
! GGTTCTGATTAGGTATTTGA+GGG - chr4_4:35685904-35685923 None:intergenic 35.0%
!! TGGTTCTGATTAGGTATTTG+AGG - chr4_4:35685905-35685924 None:intergenic 35.0%
GTATTGCCAATAGTTGTGAA+GGG - chr4_4:35685970-35685989 None:intergenic 35.0%
GCTTCTCTTACTTCCATTAT+TGG + chr4_4:35686055-35686074 Msa0678880:CDS 35.0%
TAGCTGAGTATCACCAATAA+TGG - chr4_4:35686071-35686090 None:intergenic 35.0%
ACATGTAGCAACAACAATGA+TGG + chr4_4:35686193-35686212 Msa0678880:CDS 35.0%
CATGTAGCAACAACAATGAT+GGG + chr4_4:35686194-35686213 Msa0678880:CDS 35.0%
ACTTAGTCCCAACGAAAACA+TGG + chr4_4:35682329-35682348 Msa0678880:CDS 40.0%
! GTAGCATCCATGTTTTCGTT+GGG - chr4_4:35682339-35682358 None:intergenic 40.0%
! TGTAGCATCCATGTTTTCGT+TGG - chr4_4:35682340-35682359 None:intergenic 40.0%
TGGAGGAGTTAATGATGACT+TGG - chr4_4:35682380-35682399 None:intergenic 40.0%
AGGCAAAGACAACTTCATGA+TGG - chr4_4:35682479-35682498 None:intergenic 40.0%
AGCTTGAGTTCCAACAAAGA+TGG - chr4_4:35682537-35682556 None:intergenic 40.0%
TATGAGTGTCACACATTGAC+GGG - chr4_4:35682965-35682984 None:intergenic 40.0%
GTATGAGTGTCACACATTGA+CGG - chr4_4:35682966-35682985 None:intergenic 40.0%
CAATGTGTGACACTCATACA+AGG + chr4_4:35682967-35682986 Msa0678880:intron 40.0%
TAAAATCGAAGTGAGAGCCT+TGG - chr4_4:35683020-35683039 None:intergenic 40.0%
! CCTCCGCTACTCAAATTTTT+CGG + chr4_4:35683613-35683632 Msa0678880:intron 40.0%
! CTCCGCTACTCAAATTTTTC+GGG + chr4_4:35683614-35683633 Msa0678880:intron 40.0%
GACCCGAAAAATTTGAGTAG+CGG - chr4_4:35683619-35683638 None:intergenic 40.0%
GTATCATATGGGTGCAATGA+CGG - chr4_4:35683641-35683660 None:intergenic 40.0%
CGTGCGAATAAAACATGTCA+GGG - chr4_4:35683979-35683998 None:intergenic 40.0%
ACGTGCGAATAAAACATGTC+AGG - chr4_4:35683980-35683999 None:intergenic 40.0%
!! ATGTTTTATTCGCACGTGAG+AGG + chr4_4:35683983-35684002 Msa0678880:intron 40.0%
GGGAAAAAGAGAACTGAGAA+TGG + chr4_4:35684236-35684255 Msa0678880:CDS 40.0%
! AATTGAGAATGGTGGAGAGT+TGG + chr4_4:35684472-35684491 Msa0678880:CDS 40.0%
CCTAACTGATCCTTACTTCT+AGG - chr4_4:35684584-35684603 None:intergenic 40.0%
CCTAGAAGTAAGGATCAGTT+AGG + chr4_4:35684581-35684600 Msa0678880:CDS 40.0%
GCAAGAGTTACTATTAGAGC+TGG + chr4_4:35684701-35684720 Msa0678880:CDS 40.0%
! AGCTGGATTAGAAGAAACCA+TGG + chr4_4:35684718-35684737 Msa0678880:CDS 40.0%
! AGATCACTGATGGATGTCAA+TGG + chr4_4:35684838-35684857 Msa0678880:intron 40.0%
CATGTTTGTGACTTCGATCT+AGG - chr4_4:35685140-35685159 None:intergenic 40.0%
!! GTGTTGACTGTGTAAATGCA+TGG - chr4_4:35685379-35685398 None:intergenic 40.0%
CACCTTTGTACCTGTTAAGT+TGG - chr4_4:35685608-35685627 None:intergenic 40.0%
GCAGAACTCTGAGTGATTAA+AGG - chr4_4:35685860-35685879 None:intergenic 40.0%
TTGAGGGATTATAGAAGCTG+AGG - chr4_4:35685888-35685907 None:intergenic 40.0%
GGTATTGCCAATAGTTGTGA+AGG - chr4_4:35685971-35685990 None:intergenic 40.0%
AGAGAAGCTAACAAATCTGC+AGG - chr4_4:35686043-35686062 None:intergenic 40.0%
!! TGCTATTTCCTTTGGTGACA+TGG - chr4_4:35686126-35686145 None:intergenic 40.0%
GTCACCAAAGGAAATAGCAA+TGG + chr4_4:35686127-35686146 Msa0678880:CDS 40.0%
!! GCTACCATTGCTATTTCCTT+TGG - chr4_4:35686134-35686153 None:intergenic 40.0%
GTCGCAGAAGACAATAGAAA+TGG + chr4_4:35686163-35686182 Msa0678880:CDS 40.0%
ATGATCATTGTCAGTGGCAG+AGG - chr4_4:35682499-35682518 None:intergenic 45.0%
ATAGACAACAATGTGGGCCA+AGG + chr4_4:35683000-35683019 Msa0678880:intron 45.0%
! AAAATGGGTGGCAGCTCTAA+TGG - chr4_4:35683176-35683195 None:intergenic 45.0%
CCGAAAAATTTGAGTAGCGG+AGG - chr4_4:35683616-35683635 None:intergenic 45.0%
! ACTGAGAATGGTGGAGTACT+AGG + chr4_4:35684248-35684267 Msa0678880:CDS 45.0%
! CTGAGAATGGTGGAGTACTA+GGG + chr4_4:35684249-35684268 Msa0678880:CDS 45.0%
TACTAATGCTGAGACTGCGA+TGG + chr4_4:35684526-35684545 Msa0678880:CDS 45.0%
! TCTAGGTTTTCTCATCGACG+AGG - chr4_4:35684567-35684586 None:intergenic 45.0%
!! AAGCATCGAGGTTGCTTTGA+AGG + chr4_4:35684616-35684635 Msa0678880:CDS 45.0%
GGATGTCAATGGAGAAAGTG+CGG + chr4_4:35684849-35684868 Msa0678880:CDS 45.0%
TGCGGACAGAAATTGTCGAA+AGG + chr4_4:35684867-35684886 Msa0678880:CDS 45.0%
! TTTTCGGACTGAGCAACCAT+GGG - chr4_4:35684931-35684950 None:intergenic 45.0%
GTTGTAAATGGAGCAGAAGC+TGG - chr4_4:35685773-35685792 None:intergenic 45.0%
! GGGTTCCTTTGTGGAGTATT+TGG - chr4_4:35685821-35685840 None:intergenic 45.0%
AACTGATCTGGGTTCCTTTG+TGG - chr4_4:35685830-35685849 None:intergenic 45.0%
CAAATGTCAAACGATGCAGC+TGG + chr4_4:35685944-35685963 Msa0678880:CDS 45.0%
AACGACAACCATGTCACCAA+AGG + chr4_4:35686115-35686134 Msa0678880:CDS 45.0%
!! ATTATTATACTTATAAATAT+AGG - chr4_4:35683145-35683164 None:intergenic 5.0%
!! ATGGTGGAGAGTTGGTGCAA+AGG + chr4_4:35684480-35684499 Msa0678880:CDS 50.0%
!! ATCGGCGATGAAAAGCATCG+AGG + chr4_4:35684604-35684623 Msa0678880:CDS 50.0%
TATCGCTGCAACACATCCCA+TGG + chr4_4:35684912-35684931 Msa0678880:CDS 50.0%
! GTTTTCGGACTGAGCAACCA+TGG - chr4_4:35684932-35684951 None:intergenic 50.0%
TGGTTGCTCAGTCCGAAAAC+AGG + chr4_4:35684932-35684951 Msa0678880:CDS 50.0%
ACCTGCAGCAGCCAAAGAAA+TGG + chr4_4:35685685-35685704 Msa0678880:CDS 50.0%
AGCTGGCCCTTCACAACTAT+TGG + chr4_4:35685961-35685980 Msa0678880:CDS 50.0%
! GTGTCACACATTGACGGGTC+CGG - chr4_4:35682960-35682979 None:intergenic 55.0%
CTGCAGGTAACAGGTGGTTG+TGG - chr4_4:35685673-35685692 None:intergenic 55.0%
CTTTGGCTGCTGCAGGTAAC+AGG - chr4_4:35685682-35685701 None:intergenic 55.0%
GCCATTTCTTTGGCTGCTGC+AGG - chr4_4:35685689-35685708 None:intergenic 55.0%
TGGCTGCTGCAGGTAACAGG+TGG - chr4_4:35685679-35685698 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 35682291 35686225 35682291 ID=Msa0678880;Name=Msa0678880
chr4_4 mRNA 35682291 35686225 35682291 ID=Msa0678880-mRNA-1;Parent=Msa0678880;Name=Msa0678880-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|1|5|0|553
chr4_4 exon 35682291 35682560 35682291 ID=Msa0678880-mRNA-1:exon:8446;Parent=Msa0678880-mRNA-1
chr4_4 exon 35682729 35682827 35682729 ID=Msa0678880-mRNA-1:exon:8447;Parent=Msa0678880-mRNA-1
chr4_4 exon 35684170 35684739 35684170 ID=Msa0678880-mRNA-1:exon:8448;Parent=Msa0678880-mRNA-1
chr4_4 exon 35684840 35684953 35684840 ID=Msa0678880-mRNA-1:exon:8449;Parent=Msa0678880-mRNA-1
chr4_4 exon 35685617 35686225 35685617 ID=Msa0678880-mRNA-1:exon:8450;Parent=Msa0678880-mRNA-1
chr4_4 CDS 35682291 35682560 35682291 ID=Msa0678880-mRNA-1:cds;Parent=Msa0678880-mRNA-1
chr4_4 CDS 35682729 35682827 35682729 ID=Msa0678880-mRNA-1:cds;Parent=Msa0678880-mRNA-1
chr4_4 CDS 35684170 35684739 35684170 ID=Msa0678880-mRNA-1:cds;Parent=Msa0678880-mRNA-1
chr4_4 CDS 35684840 35684953 35684840 ID=Msa0678880-mRNA-1:cds;Parent=Msa0678880-mRNA-1
chr4_4 CDS 35685617 35686225 35685617 ID=Msa0678880-mRNA-1:cds;Parent=Msa0678880-mRNA-1
Gene Sequence

>Msa0678880

ATGGATTCAAATCTAGTTAATAACAACCTTGAGTTAAAACTTAGTCCCAACGAAAACATGGATGCTACACATAGTACTATCCCTTTCCAAGTCATCATTAACTCCTCCAATGAAAATCAAACCTCTCAAATCTCATCTCATGAAACAGGAATAGAGTTAGATTTCTTTAAGGACAACAACAATGACCATCATGAAGTTGTCTTTGCCTCTGCCACTGACAATGATCATATTCATACTGATACACCATCTTTGTTGGAACTCAAGCTGAGTATTGGTCCCAATCATGTTACTACCAATACTAGTAGTGATCAATCATTGATGGATGATGGCATGCCACATAATTTAGAATACAAAAAAACCAAGAGGGAGAAAATGATGCAAGACCATAATGTTAAGGAAGTTAAAGGAAAAGAAGTATTTGATGGAAACTTTAAAGGGAAAAAGAGAACTGAGAATGGTGGAGTACTAGGGCCAATGAAATTTATGGATCTAAAATTGGCTAGTAATAAGGGTAAGGAAGTTAAAGGACAAGAAGTTTCTGATGGAAAATTTGGAGACAAAAAGAGAATGAATAATGATGGAGAATTAGTGAAAAGGAAATTTGTTGATGCTGGATTGGATACTAATAAGGTTAAAGAAGTGTTTAATGGAAAATGTGAAAAGAAAAAGAGAATTGAGAATGGTGGAGAGTTGGTGCAAAGGCAATGTAGTGATTTTGTGTTGATTACTAATGCTGAGACTGCGATGGATCATGAAGCTTCTTCCTCGTCGATGAGAAAACCTAGAAGTAAGGATCAGTTAGGATCGGCGATGAAAAGCATCGAGGTTGCTTTGAAGGAGTTAGTTTTAAGTAAGAATGCGGATTTTAATGTTGATAATGCTGAGGCTACATTGACAAAAGCAAGAGTTACTATTAGAGCTGGATTAGAAGAAACCATGATCACTGATGGATGTCAATGGAGAAAGTGCGGACAGAAATTGTCGAAAGGAAACCCATGTCCTAAAGCTTATTATCGCTGCAACACATCCCATGGTTGCTCAGTCCGAAAACAGGTACAAAGGTGTGATCTTGATAGAACAGTTGTCAACACAACATATGAAGAAAACCACAACCACCTGTTACCTGCAGCAGCCAAAGAAATGGCAAAAACAACTTCAGCAGCTGCAAAGATGCTCCTTCCTTGTTCTTCAAGTATTGCAACTATACCAGCTTCTGCTCCATTTACAACAATTACACGTGATTATACTCAATCTCCAAATACTCCACAAAGGAACCCAGATCAGTTTCAAACTCCTTTAATCACTCAGAGTTCTGCAAATTCCTCAGCTTCTATAATCCCTCAAATACCTAATCAGAACCAATCAAAATTTTCTGGACTACAAATGTCAAACGATGCAGCTGGCCCTTCACAACTATTGGCAATACCAAATATTGTTCAGATAGTTAATGCTGCCATTGCTGCTAATCCAAATTTTCCTGCAGATTTGTTAGCTTCTCTTACTTCCATTATTGGTGATACTCAGCTAAATAACACTCTTGCTGATAACAATAACGACAACCATGTCACCAAAGGAAATAGCAATGGTAGCAACAATAATGTCGCAGAAGACAATAGAAATGGAAACATCACATGTAGCAACAACAATGATGGGAAGAAATAA
Protein sequence

>Msa0678880

MDSNLVNNNLELKLSPNENMDATHSTIPFQVIINSSNENQTSQISSHETGIELDFFKDNNNDHHEVVFASATDNDHIHTDTPSLLELKLSIGPNHVTTNTSSDQSLMDDGMPHNLEYKKTKREKMMQDHNVKEVKGKEVFDGNFKGKKRTENGGVLGPMKFMDLKLASNKGKEVKGQEVSDGKFGDKKRMNNDGELVKRKFVDAGLDTNKVKEVFNGKCEKKKRIENGGELVQRQCSDFVLITNAETAMDHEASSSSMRKPRSKDQLGSAMKSIEVALKELVLSKNADFNVDNAEATLTKARVTIRAGLEETMITDGCQWRKCGQKLSKGNPCPKAYYRCNTSHGCSVRKQVQRCDLDRTVVNTTYEENHNHLLPAAAKEMAKTTSAAAKMLLPCSSSIATIPASAPFTTITRDYTQSPNTPQRNPDQFQTPLITQSSANSSASIIPQIPNQNQSKFSGLQMSNDAAGPSQLLAIPNIVQIVNAAIAANPNFPADLLASLTSIIGDTQLNNTLADNNNDNHVTKGNSNGSNNNVAEDNRNGNITCSNNNDGKK*