AlfalfaGEDB | Msa0680260

M. sativa cultivar zhongmu-4 / Msa0680260

KEGG: K12412
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009791 GO:0009888 GO:0009889 GO:0009960 GO:0010154 GO:0010468 GO:0010556 GO:0010817 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032879 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051049 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0080090 GO:0140110 GO:1903506 GO:2000012 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0680260	XP_013455733.1	79.583	240	39	4	1	236	1	234	3.56e-120	352

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0680260	sp\|Q9FKK2\|AGL62_ARATH	28.879	232	128	10	7	236	5	201	2.89e-13	70.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0680260	A0A072UJ33	79.583	240	39	4	1	236	1	234	1.70e-120	352

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TFs/TRs:

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Gene ID	Type	Classification
Msa0680260	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0680260	MtrunA17_Chr4g0025531	79.583	240	39	4	1	236	1	234	3.28e-124	352
Msa0680260	MtrunA17_Chr3g0135891	51.128	133	43	4	106	222	16	142	2.92e-32	115
Msa0680260	MtrunA17_Chr5g0422941	24.696	247	150	6	1	226	1	232	2.76e-17	78.2
Msa0680260	MtrunA17_Chr1g0208561	27.811	169	109	2	1	159	1	166	3.55e-16	74.7
Msa0680260	MtrunA17_Chr8g0355091	38.043	92	57	0	1	92	1	92	4.61e-16	74.7
Msa0680260	MtrunA17_Chr2g0317481	24.370	238	162	5	1	226	1	232	7.13e-16	74.3
Msa0680260	MtrunA17_Chr3g0099421	28.249	177	111	3	1	164	26	199	9.50e-16	74.3
Msa0680260	MtrunA17_Chr3g0099411	38.043	92	57	0	1	92	1	92	1.00e-15	73.6
Msa0680260	MtrunA17_Chr3g0119901	43.529	85	47	1	1	85	1	84	1.09e-15	73.2
Msa0680260	MtrunA17_Chr1g0206701	38.043	92	57	0	1	92	1	92	1.12e-15	73.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0680260	AT1G29962.1	43.750	80	44	1	1	80	1	79	3.82e-16	74.3
Msa0680260	AT4G36590.1	44.118	68	38	0	5	72	3	70	1.24e-15	74.3
Msa0680260	AT5G60440.1	28.879	232	128	10	7	236	5	201	2.94e-14	70.9
Msa0680260	AT2G34440.1	38.272	81	48	1	9	87	2	82	4.53e-14	68.6
Msa0680260	AT2G24840.1	41.558	77	45	0	7	83	61	137	5.94e-14	69.7
Msa0680260	AT1G28460.1	42.308	78	44	1	1	78	1	77	2.00e-13	67.0
Msa0680260	AT1G01530.1	34.694	98	60	1	5	98	3	100	3.46e-12	64.7
Msa0680260	AT3G04100.1	35.294	85	52	1	2	86	7	88	1.33e-11	62.4
Msa0680260	AT3G66656.1	40.000	65	39	0	9	73	2	66	2.09e-11	61.2
Msa0680260	AT1G28450.1	38.750	80	48	1	1	80	1	79	2.47e-11	61.2

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Find 43 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTCTGGAATTTACAACA+AGG	0.757794	4_4:-39249301	Msa0680260:CDS
AAATCCTCCTTATGTCATTG+AGG	0.570912	4_4:-39248728	Msa0680260:CDS
AACCTTATTGGTGCAAGCGG+AGG	0.596051	4_4:-39248474	Msa0680260:CDS
AAGATGAACACTTACCACGA+GGG	0.703390	4_4:+39248893	None:intergenic
AAGCATGAGAATATCAACTG+AGG	0.718686	4_4:+39249251	None:intergenic
ACACAAGGAGTCAACCTTAT+TGG	0.323352	4_4:-39248486	Msa0680260:CDS
AGAAGAGGAAATGATGAAAC+TGG	0.357197	4_4:-39249142	Msa0680260:CDS
ATCAACAAAGATCATGAAGT+TGG	0.522438	4_4:-39248703	Msa0680260:CDS
ATCATTTCCTCTTCTGTTGA+AGG	0.293016	4_4:+39249150	None:intergenic
ATCCTCCGCTTGCACCAATA+AGG	0.357163	4_4:+39248472	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCAACAAAGATCATGAAGT+TGG	-	chr4_4:39249124-39249143	Msa0680260:CDS	30.0%
TTATCCAAAAGGTAAAGGTA+TGG	-	chr4_4:39249292-39249311	Msa0680260:CDS	30.0%
TCTCATGATATAGAAACACA+AGG	-	chr4_4:39249326-39249345	Msa0680260:CDS	30.0%
ATCAACAAAGATCATGAAGT+TGG	-	chr4_4:39249124-39249143	Msa0680260:CDS	30.0%
TTATCCAAAAGGTAAAGGTA+TGG	-	chr4_4:39249292-39249311	Msa0680260:CDS	30.0%
TCTCATGATATAGAAACACA+AGG	-	chr4_4:39249326-39249345	Msa0680260:CDS	30.0%
TCTGAGTTAACAACACTTTC+AGG	-	chr4_4:39248551-39248570	Msa0680260:intron	35.0%
CTGAGTTAACAACACTTTCA+GGG	-	chr4_4:39248552-39248571	Msa0680260:intron	35.0%
TGAGTTAACAACACTTTCAG+GGG	-	chr4_4:39248553-39248572	Msa0680260:intron	35.0%
AAGCATGAGAATATCAACTG+AGG	+	chr4_4:39248579-39248598	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	39248434	39249415	39248434	ID=Msa0680260;Name=Msa0680260
chr4_4	mRNA	39248434	39249415	39248434	ID=Msa0680260-mRNA-1;Parent=Msa0680260;Name=Msa0680260-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0\|0\|0\|1\|1\|1\|3\|0\|236
chr4_4	exon	39248908	39249415	39248908	ID=Msa0680260-mRNA-1:exon:9244;Parent=Msa0680260-mRNA-1
chr4_4	exon	39248698	39248787	39248698	ID=Msa0680260-mRNA-1:exon:9243;Parent=Msa0680260-mRNA-1
chr4_4	exon	39248434	39248546	39248434	ID=Msa0680260-mRNA-1:exon:9242;Parent=Msa0680260-mRNA-1
chr4_4	CDS	39248908	39249415	39248908	ID=Msa0680260-mRNA-1:cds;Parent=Msa0680260-mRNA-1
chr4_4	CDS	39248698	39248787	39248698	ID=Msa0680260-mRNA-1:cds;Parent=Msa0680260-mRNA-1
chr4_4	CDS	39248434	39248546	39248434	ID=Msa0680260-mRNA-1:cds;Parent=Msa0680260-mRNA-1

Gene Sequence

>Msa0680260

ATGGCGCCGAAGAGGCCATCAAGAGGAAAACAAAAAGTAAAGCTGAAAAAAATTGAGAGTAAGAATGATCTCTTTGTTGCATTTACAACGAGAAAGTCTGGAATTTACAACAAGGCCTCTGAGTTAACAACACTTTCAGGGGCCTCAGTTGATATTCTCATGCTTTCTCCAACAGGGAAACCATTTTGTTATGGAGAACCATCTTCTGAATCTATAACAAAAACTAGTTTGAATGATGAAAATCCTTCAACAGAAGAGGAAATGATGAAACTGGAGATTATACAGCAAAATGAAAAAAATGATGATCATTTGGATAAAAAGCATGTTGCGGAAGCACAATGTAAAAATTTGAAGATGTTGGAAAGTTCTGGATATTGGTCGATACAAGAGGAAGATGTTAAAAGCTACCAATATGATCAAGTGAAAGAAATTGACAAATCCTTAGTTGCTGTCGCAAATAAATTGATAAATAATGTCTTGAAAAATGGAGGAGAGCTTAACCCTCGTGTAATTTCTACCATCGAGGAACTGAATGGAGTCAAAACAAATCCTCCTTATGTCATTGAGGACATCAACAAAGATCATGAAGTTGGTGCAAGTAAAGGTATGGTGGCTAATCCTTCTCATGATATAGAAACACAAGGAGTCAACCTTATTGGTGCAAGCGGAGGATATGGAAGTGGTGTTGATCATGCTTCTCCAGATATATGA

Protein sequence

>Msa0680260

MAPKRPSRGKQKVKLKKIESKNDLFVAFTTRKSGIYNKASELTTLSGASVDILMLSPTGKPFCYGEPSSESITKTSLNDENPSTEEEMMKLEIIQQNEKNDDHLDKKHVAEAQCKNLKMLESSGYWSIQEEDVKSYQYDQVKEIDKSLVAVANKLINNVLKNGGELNPRVISTIEELNGVKTNPPYVIEDINKDHEVGASKGMVANPSHDIETQGVNLIGASGGYGSGVDHASPDI*