AlfalfaGEDB | Msa0681400

M. sativa cultivar zhongmu-4 / Msa0681400

KEGG: K11276
GO: -
PFAM: PF13894.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0681400	KAH1104382.1	44.560	193	50	7	78	217	6	194	3.16e-34	132

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0681400	sp\|Q8LJS2\|HDT1_SOYBN	55.294	85	36	1	79	163	7	89	2.73e-20	90.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0681400	A0A072VB01	72.727	88	22	1	78	165	6	91	6.07e-30	122

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TFs/TRs:

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Gene ID	Type	Classification
Msa0681400	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0681400	MtrunA17_Chr2g0320884	67.021	94	29	1	78	171	40	131	4.56e-31	117
Msa0681400	MtrunA17_Chr2g0320884	61.017	59	19	2	163	217	271	329	6.03e-14	70.1
Msa0681400	MtrunA17_Chr4g0010251	74.242	66	15	2	1	66	691	754	2.65e-22	95.5
Msa0681400	MtrunA17_Chr4g0009781	74.242	66	15	2	1	66	696	759	5.31e-22	94.7
Msa0681400	MtrunA17_Chr4g0010221	74.242	66	15	2	1	66	694	757	5.89e-22	94.7
Msa0681400	MtrunA17_Chr4g0010201	72.727	66	16	2	1	66	695	758	4.74e-20	89.0
Msa0681400	MtrunA17_Chr4g0010261	71.212	66	18	1	1	66	696	760	7.51e-20	88.6
Msa0681400	MtrunA17_Chr4g0027191	51.064	94	44	1	79	172	11	102	2.58e-19	85.9
Msa0681400	MtrunA17_Chr4g0027181	46.809	94	48	1	79	172	11	102	1.00e-17	80.1
Msa0681400	MtrunA17_Chr7g0265761	43.902	82	42	2	79	160	7	84	5.11e-13	67.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 51 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCATACCTGCTGCAATTA+CGG	0.369962	4_4:-41734365	None:intergenic
AAGGCCAAGCATGCTGCTAG+AGG	0.601545	4_4:+41734507	Msa0681400:CDS
AATACGAGGTAAATAACCAC+AGG	0.651072	4_4:-41733590	None:intergenic
AATTGGAACGAACACACTGT+TGG	0.632075	4_4:+41731315	Msa0681400:CDS
ACAACTCCTGATAAATGGTT+TGG	0.425960	4_4:+41731215	Msa0681400:CDS
ACTTCACTTAGTAGCATGCT+TGG	0.535966	4_4:-41734684	None:intergenic
AGAAAATGTCATATTGATAT+TGG	0.320848	4_4:-41731397	None:intergenic
AGCAGCTATTCACATTTCTC+AGG	0.458967	4_4:+41733414	Msa0681400:CDS
AGGACATTCACCTCTGAAAA+TGG	0.285743	4_4:+41734468	Msa0681400:intron
AGGAGACAACTCCTGATAAA+TGG	0.290359	4_4:+41731210	Msa0681400:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTAAAGCACAAAATGTTGAT+GGG	-	chr4_4:41731504-41731523	None:intergenic	30.0%
ACTAAAGCACAAAATGTTGA+TGG	-	chr4_4:41731505-41731524	None:intergenic	30.0%
TGACAGATATAGATCTTGAT+AGG	+	chr4_4:41731524-41731543	Msa0681400:intron	30.0%
GATAGGTTAACAAATCTCAA+AGG	+	chr4_4:41731541-41731560	Msa0681400:intron	30.0%
GAATTTATCATGTTCTCTTC+AGG	+	chr4_4:41731688-41731707	Msa0681400:intron	30.0%
TGACGAAATATGATTGTTGT+AGG	+	chr4_4:41731797-41731816	Msa0681400:intron	30.0%
AGGAAATATGTATCAGCAAT+TGG	+	chr4_4:41731830-41731849	Msa0681400:intron	30.0%
AAGTTCATAAGAACCTCTTT+GGG	-	chr4_4:41732044-41732063	None:intergenic	30.0%
AGTACACCAATATATCTCAT+AGG	-	chr4_4:41732311-41732330	None:intergenic	30.0%
AGCATTAAAAGTCCACTTAT+GGG	-	chr4_4:41732391-41732410	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	41731207	41734703	41731207	ID=Msa0681400;Name=Msa0681400
chr4_4	mRNA	41731207	41734703	41731207	ID=Msa0681400-mRNA-1;Parent=Msa0681400;Name=Msa0681400-mRNA-1;_AED=0.44;_eAED=0.46;_QI=0\|0\|0\|0.83\|0.6\|0.66\|6\|0\|245
chr4_4	exon	41731207	41731435	41731207	ID=Msa0681400-mRNA-1:exon:9940;Parent=Msa0681400-mRNA-1
chr4_4	exon	41733359	41733441	41733359	ID=Msa0681400-mRNA-1:exon:9941;Parent=Msa0681400-mRNA-1
chr4_4	exon	41733551	41733725	41733551	ID=Msa0681400-mRNA-1:exon:9942;Parent=Msa0681400-mRNA-1
chr4_4	exon	41734274	41734379	41734274	ID=Msa0681400-mRNA-1:exon:9943;Parent=Msa0681400-mRNA-1
chr4_4	exon	41734470	41734553	41734470	ID=Msa0681400-mRNA-1:exon:9944;Parent=Msa0681400-mRNA-1
chr4_4	exon	41734643	41734703	41734643	ID=Msa0681400-mRNA-1:exon:9945;Parent=Msa0681400-mRNA-1
chr4_4	CDS	41731207	41731435	41731207	ID=Msa0681400-mRNA-1:cds;Parent=Msa0681400-mRNA-1
chr4_4	CDS	41733359	41733441	41733359	ID=Msa0681400-mRNA-1:cds;Parent=Msa0681400-mRNA-1
chr4_4	CDS	41733551	41733725	41733551	ID=Msa0681400-mRNA-1:cds;Parent=Msa0681400-mRNA-1
chr4_4	CDS	41734274	41734379	41734274	ID=Msa0681400-mRNA-1:cds;Parent=Msa0681400-mRNA-1
chr4_4	CDS	41734470	41734553	41734470	ID=Msa0681400-mRNA-1:cds;Parent=Msa0681400-mRNA-1
chr4_4	CDS	41734643	41734703	41734643	ID=Msa0681400-mRNA-1:cds;Parent=Msa0681400-mRNA-1

Gene Sequence

>Msa0681400

TTGAGGAGACAACTCCTGATAAATGGTTTGGAATGTCTGAGGCATTGTCCAACAGAAGCTAAGCATATGAATTTAATTGGGAAGTTACATTTTCATTGGTTGATATTGAATTGGAACGAACACACTGTTGGTGATGCCAATGATGAGTTGGAGAAAGATGATATAATTCTACATGAAATAGGTCTGCACTCCAATATCAATATGACATTTTCTAGTTCGAACTCTCATTCTGCCGAGGTTAAGGTTGGAGAAACTATCAAGATTGACCCGTATGCCCATGATGCAGCAGCTATTCACATTTCTCAGGTTAGGGTAGCACTTGGCGCAGCAAATAAAGGCATACCAGAAGAGCCTGTGGTTATTTACCTCGTATTTGGTGGCCAGAAGATTGTTTTGGGAACCTTAATCAGGGACAAAATTCCTCAGATAACTTTTAATAATATTCTTGAAAAGGAATTTGAGCTTTCTCACGATTCCAAAACTGCGAATTCTGAGAAAGCTGGACATACAGCAATTCCTGACTCTAAGAAAGGTCCTAACAATGATGCTGAAACTTCCAAGTCTGGAGGACTCTCTTGCCGTAATTGCAGCAGGACATTCACCTCTGAAAATGGATTGCAAGAACATATAAAGGCCAAGCATGCTGCTAGAGGACTCTGTTGCAGAAGTTGCAAGAGGACATTCACCTCTGAAAATGCATTGCAAGAACATAACAAGGCCAAGCATGCTACTAAGTGA

Protein sequence

>Msa0681400

LRRQLLINGLECLRHCPTEAKHMNLIGKLHFHWLILNWNEHTVGDANDELEKDDIILHEIGLHSNINMTFSSSNSHSAEVKVGETIKIDPYAHDAAAIHISQVRVALGAANKGIPEEPVVIYLVFGGQKIVLGTLIRDKIPQITFNNILEKEFELSHDSKTANSEKAGHTAIPDSKKGPNNDAETSKSGGLSCRNCSRTFTSENGLQEHIKAKHAARGLCCRSCKRTFTSENALQEHNKAKHATK*