AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0684820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0684820 MtrunA17_Chr4g0025531 54.980 251 92 5 1 248 1 233 2.07e-81 244
Msa0684820 MtrunA17_Chr3g0135891 56.757 148 57 5 94 238 3 146 3.56e-42 140
Msa0684820 MtrunA17_Chr2g0292791 37.126 167 88 4 1 150 1 167 8.05e-22 90.5
Msa0684820 MtrunA17_Chr1g0206701 36.646 161 85 4 1 144 1 161 8.83e-22 90.5
Msa0684820 MtrunA17_Chr1g0149391 37.356 174 87 8 1 156 1 170 9.70e-22 90.5
Msa0684820 MtrunA17_Chr3g0099421 36.527 167 89 5 1 150 26 192 1.34e-21 90.5
Msa0684820 MtrunA17_Chr8g0355091 36.424 151 81 3 1 136 1 151 2.41e-21 89.4
Msa0684820 MtrunA17_Chr5g0422421 32.075 212 116 8 1 201 1 195 4.97e-21 88.6
Msa0684820 MtrunA17_Chr1g0188671 30.457 197 113 5 1 187 39 221 9.49e-21 88.6
Msa0684820 MtrunA17_Chr1g0206991 36.646 161 85 5 1 144 1 161 1.04e-20 86.3
Msa0684820 MtrunA17_Chr1g0208561 32.530 166 99 3 1 156 1 163 1.46e-20 87.0
Msa0684820 MtrunA17_Chr3g0089141 32.530 166 99 3 1 156 1 163 2.43e-20 86.3
Msa0684820 MtrunA17_Chr3g0119901 33.133 166 96 4 1 155 1 162 1.34e-19 84.0
Msa0684820 MtrunA17_Chr1g0206721 35.762 151 82 3 1 136 1 151 2.27e-19 84.0
Msa0684820 MtrunA17_Chr2g0317451 30.625 160 100 3 1 151 1 158 3.79e-19 83.6
Msa0684820 MtrunA17_Chr2g0317481 30.625 160 100 3 1 151 1 158 4.11e-19 83.2
Msa0684820 MtrunA17_Chr8g0379021 31.579 171 84 4 4 156 7 162 9.54e-19 80.9
Msa0684820 MtrunA17_Chr1g0187361 30.682 176 106 4 1 163 1 173 1.18e-18 82.0
Msa0684820 MtrunA17_Chr3g0085241 34.615 156 87 4 4 148 36 187 1.72e-18 82.0
Msa0684820 MtrunA17_Chr3g0099411 51.389 72 35 0 1 72 1 72 2.03e-18 80.9
Msa0684820 MtrunA17_Chr5g0419601 33.577 137 81 3 1 127 1 137 2.05e-18 81.3
Msa0684820 MtrunA17_Chr1g0208581 34.132 167 81 6 1 144 1 161 2.31e-18 81.3
Msa0684820 MtrunA17_Chr2g0333981 30.818 159 101 3 4 156 7 162 1.29e-17 77.8
Msa0684820 MtrunA17_Chr5g0422941 29.536 237 127 10 1 202 1 232 1.59e-17 79.0
Msa0684820 MtrunA17_Chr2g0325971 31.677 161 93 4 1 144 1 161 8.14e-17 77.0
Msa0684820 MtrunA17_Chr3g0111841 51.724 58 28 0 15 72 2 59 1.72e-15 69.3
Msa0684820 MtrunA17_Chr3g0089151 39.252 107 62 2 1 104 1 107 2.03e-15 70.9
Msa0684820 MtrunA17_Chr4g0052081 49.275 69 35 0 9 77 2 70 2.18e-15 72.0
Msa0684820 MtrunA17_Chr2g0300421 44.444 72 40 0 1 72 1 72 4.04e-15 71.2
Msa0684820 MtrunA17_Chr4g0052061 34.028 144 83 4 9 151 2 134 2.11e-14 68.9
Msa0684820 MtrunA17_Chr1g0169761 36.885 122 61 3 9 118 2 119 2.30e-14 69.3
Msa0684820 MtrunA17_Chr1g0206691 29.189 185 112 6 18 185 1 183 5.73e-14 68.9
Msa0684820 MtrunA17_Chr8g0356071 41.667 72 42 0 9 80 2 73 6.18e-14 67.8
Msa0684820 MtrunA17_Chr8g0357771 32.143 140 71 4 9 126 2 139 7.16e-14 67.8
Msa0684820 MtrunA17_Chr8g0376821 41.250 80 46 1 9 87 2 81 1.21e-13 67.4
Msa0684820 MtrunA17_Chr2g0300441 30.120 166 99 4 1 149 1 166 1.35e-13 68.2
Msa0684820 MtrunA17_Chr8g0345001 32.515 163 94 4 8 159 17 174 3.47e-13 66.2
Msa0684820 MtrunA17_Chr1g0180591 36.134 119 66 3 9 118 2 119 1.36e-12 64.3
Msa0684820 MtrunA17_Chr4g0074101 38.889 72 44 0 9 80 2 73 4.77e-12 63.2
Msa0684820 MtrunA17_Chr1g0175611 40.000 70 42 0 9 78 2 71 4.83e-12 62.8
Msa0684820 MtrunA17_Chr1g0159661 48.438 64 33 0 9 72 2 65 2.09e-11 61.2
Msa0684820 MtrunA17_Chr4g0018951 29.921 127 83 4 26 148 29 153 4.47e-11 60.1
Msa0684820 MtrunA17_Chr1g0175601 40.000 65 39 0 9 73 2 66 9.49e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0684820 AT4G36590.1 34.132 167 93 3 5 156 3 167 2.41e-21 90.1
Msa0684820 AT1G29962.1 35.227 176 96 4 1 161 1 173 2.62e-21 88.2
Msa0684820 AT2G24840.1 32.474 194 109 6 7 187 61 245 1.52e-20 88.2
Msa0684820 AT1G28460.1 35.260 173 97 4 1 161 1 170 2.02e-19 83.2
Msa0684820 AT5G60440.1 32.547 212 115 7 5 205 3 197 8.70e-18 81.3
Msa0684820 AT1G01530.1 37.288 118 68 2 5 117 3 119 1.13e-17 80.1
Msa0684820 AT2G34440.1 32.203 177 97 7 9 174 2 166 5.86e-17 76.3
Msa0684820 AT1G28450.1 32.759 174 99 4 1 159 1 171 1.83e-16 75.5
Msa0684820 AT3G04100.1 41.573 89 49 1 2 90 7 92 2.01e-15 73.2
Msa0684820 AT1G65360.1 33.019 106 67 1 5 106 3 108 9.05e-15 71.6
Msa0684820 AT3G66656.1 47.692 65 34 0 9 73 2 66 1.86e-13 67.0
Msa0684820 AT1G47760.1 39.759 83 47 1 9 91 2 81 3.85e-13 66.2
Msa0684820 AT4G11880.2 49.254 67 32 1 10 76 3 67 8.41e-11 59.7
Msa0684820 AT4G11880.3 49.254 67 32 1 10 76 3 67 8.41e-11 59.7

Find 56 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGCATATCATTCATCTTT+AGG 0.191781 4_4:-48462712 None:intergenic
ACAAATCCTCCTTATGTCTT+TGG 0.244037 4_4:+48462295 Msa0684820:CDS
AACAACAACGGTTATGAAAT+TGG 0.254239 4_4:+48462322 Msa0684820:CDS
TCAGGAAAGTTTGAAGGCTT+TGG 0.277217 4_4:+48461968 Msa0684820:CDS
CAAATCCTCCTTATGTCTTT+GGG 0.283816 4_4:+48462296 Msa0684820:CDS
ATCATATATACCTTGGAATA+AGG 0.308845 4_4:+48462755 Msa0684820:three_prime_UTR
ATAAGCGGACATCACATTTC+AGG 0.330823 4_4:+48462782 Msa0684820:three_prime_UTR
GAAGGTTCTCCATAACAATA+TGG 0.353627 4_4:-48461810 None:intergenic
CTTTGGAAAGTTATGGCTAT+TGG 0.358280 4_4:+48461985 Msa0684820:CDS
AAAATTGGACACATCTTTAG+TGG 0.370003 4_4:+48462055 Msa0684820:CDS
GATGTTATTGCTTCTCTTGA+AGG 0.392101 4_4:+48462616 Msa0684820:CDS
TCTGAATTATCAACACTTTG+TGG 0.392582 4_4:+48461747 Msa0684820:CDS
TTGAAGGCTTTGGAAAGTTA+TGG 0.393387 4_4:+48461978 Msa0684820:CDS
GCATGTTGCGGAAGCTCATC+AGG 0.403902 4_4:+48461950 Msa0684820:CDS
CTTGCTATGGATTGAGAAGA+AGG 0.408194 4_4:-48461828 None:intergenic
GCAGGAAAACCATATTGTTA+TGG 0.420932 4_4:+48461801 Msa0684820:CDS
TCACTTGCACGGTCAAGGTT+TGG 0.423194 4_4:-48462658 None:intergenic
CTGAATTATCAACACTTTGT+GGG 0.424852 4_4:+48461748 Msa0684820:CDS
CTACCTCTTGATGTCCTCTT+CGG 0.429458 4_4:-48461636 None:intergenic
CTTCAAAATTGTTCTTGCTA+TGG 0.431100 4_4:-48461841 None:intergenic
CATCAAAATGAGAAAATAGT+TGG 0.433292 4_4:-48462817 None:intergenic
GGTATATATGATCATACATA+TGG 0.438624 4_4:-48462744 None:intergenic
CATTTCCACTTTCACTTGCA+CGG 0.448306 4_4:-48462669 None:intergenic
ATTCTTATTATTTCTCCAGC+AGG 0.453510 4_4:+48461783 Msa0684820:CDS
TGAGAAAATAGTTGGAATCA+TGG 0.457232 4_4:-48462809 None:intergenic
CACTTTCACTTGCACGGTCA+AGG 0.460125 4_4:-48462663 None:intergenic
AATTTCATAACCGTTGTTGT+TGG 0.462889 4_4:-48462320 None:intergenic
AGACATAAGGAGGATTTGTC+TGG 0.473975 4_4:-48462291 None:intergenic
CTTGACCGTGCAAGTGAAAG+TGG 0.475104 4_4:+48462664 Msa0684820:CDS
TTGTTGGCCCCAAAGACATA+AGG 0.478695 4_4:-48462304 None:intergenic
TTTAATTCTTCTTCTACCGA+AGG 0.479866 4_4:-48461873 None:intergenic
AGCTCATCAGGAAAGTTTGA+AGG 0.487303 4_4:+48461962 Msa0684820:CDS
CGTGCAAGTGAAAGTGGAAA+TGG 0.489776 4_4:+48462670 Msa0684820:CDS
ATTTATGATCCAAAAGGAAA+AGG 0.506756 4_4:+48462589 Msa0684820:CDS
TCCCAGATTTATGATCCAAA+AGG 0.515467 4_4:+48462583 Msa0684820:CDS
TTTGAAGAATGAAAACCCTT+CGG 0.527588 4_4:+48461857 Msa0684820:CDS
TAAGCGGACATCACATTTCA+GGG 0.527919 4_4:+48462783 Msa0684820:three_prime_UTR
TCTTGCATAGATGAACTGAA+TGG 0.533604 4_4:+48462265 Msa0684820:CDS
TTAATTCTTCTTCTACCGAA+GGG 0.546382 4_4:-48461872 None:intergenic
ATAAGTAGTGTCTTAAGCAA+TGG 0.548667 4_4:+48462095 Msa0684820:CDS
GCTTATATCACCTTATTCCA+AGG 0.555747 4_4:-48462765 None:intergenic
ATGTATGATCATATATACCT+TGG 0.560368 4_4:+48462748 Msa0684820:three_prime_UTR
TGAATTATCAACACTTTGTG+GGG 0.569118 4_4:+48461749 Msa0684820:CDS
GTCTTTGGGGCCAACAACAA+CGG 0.574083 4_4:+48462310 Msa0684820:CDS
GGAGGAGAGATTAATCCTCA+TGG 0.604055 4_4:+48462116 Msa0684820:CDS
AAATCCTCCTTATGTCTTTG+GGG 0.607760 4_4:+48462297 Msa0684820:CDS
AGTAGTGTCTTAAGCAATGG+AGG 0.611596 4_4:+48462098 Msa0684820:CDS
TTGGCCCCAAAGACATAAGG+AGG 0.611952 4_4:-48462301 None:intergenic
ACAAAGTGTTGATAATTCAG+AGG 0.616841 4_4:-48461745 None:intergenic
TGATCCAAAAGGAAAAGGCA+AGG 0.624272 4_4:+48462594 Msa0684820:CDS
TTTGGATGAAAAGCATGTTG+CGG 0.629913 4_4:+48461938 Msa0684820:CDS
TGGTTAAGATGGCTCCGAAG+AGG 0.660173 4_4:+48461622 None:intergenic
TTGGAATAAGGTGATATAAG+CGG 0.660825 4_4:+48462767 Msa0684820:three_prime_UTR
GCTCCGAAGAGGACATCAAG+AGG 0.673948 4_4:+48461633 Msa0684820:CDS
GAAGGTAGAAATAAAACACA+AGG 0.705496 4_4:+48462634 Msa0684820:CDS
GCGGACATCACATTTCAGGG+TGG 0.773228 4_4:+48462786 Msa0684820:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATTTATTTATGAAAAAG+TGG + chr4_4:48462219-48462238 Msa0684820:intron 15.0%
!! CTAATTTATTTATGAAAAAG+TGG + chr4_4:48462219-48462238 Msa0684820:intron 15.0%
!! TTATGACCAGTTAAAAAAAT+TGG + chr4_4:48462040-48462059 Msa0684820:CDS 20.0%
!!! TACTCTTGTTTATATGAATT+TGG - chr4_4:48462368-48462387 None:intergenic 20.0%
!!! ATGTCTTCATATCTTTTTTT+GGG - chr4_4:48462494-48462513 None:intergenic 20.0%
!!! AATGTCTTCATATCTTTTTT+TGG - chr4_4:48462495-48462514 None:intergenic 20.0%
!! GAAATTATACAATATGTGTT+AGG - chr4_4:48462543-48462562 None:intergenic 20.0%
!!! TTTTCTATATTCTACTTTTG+TGG + chr4_4:48462876-48462895 Msa0684820:three_prime_UTR 20.0%
!! TTATGACCAGTTAAAAAAAT+TGG + chr4_4:48462040-48462059 Msa0684820:CDS 20.0%
!!! TACTCTTGTTTATATGAATT+TGG - chr4_4:48462368-48462387 None:intergenic 20.0%
!!! ATGTCTTCATATCTTTTTTT+GGG - chr4_4:48462494-48462513 None:intergenic 20.0%
!!! AATGTCTTCATATCTTTTTT+TGG - chr4_4:48462495-48462514 None:intergenic 20.0%
!! GAAATTATACAATATGTGTT+AGG - chr4_4:48462543-48462562 None:intergenic 20.0%
!!! TTTTCTATATTCTACTTTTG+TGG + chr4_4:48462876-48462895 Msa0684820:three_prime_UTR 20.0%
!!! ATGTGTCCAATTTTTTTAAC+TGG - chr4_4:48462049-48462068 None:intergenic 25.0%
! AGTATAGATATTTGATGTGT+TGG - chr4_4:48462409-48462428 None:intergenic 25.0%
! GCATTAAAAATGGTTAAAGT+TGG - chr4_4:48462433-48462452 None:intergenic 25.0%
! TCTCATAAGTGCATTAAAAA+TGG - chr4_4:48462443-48462462 None:intergenic 25.0%
! ATTTATGATCCAAAAGGAAA+AGG + chr4_4:48462589-48462608 Msa0684820:CDS 25.0%
! GGTATATATGATCATACATA+TGG - chr4_4:48462747-48462766 None:intergenic 25.0%
! ATGTATGATCATATATACCT+TGG + chr4_4:48462748-48462767 Msa0684820:three_prime_UTR 25.0%
! ATCATATATACCTTGGAATA+AGG + chr4_4:48462755-48462774 Msa0684820:three_prime_UTR 25.0%
! CATCAAAATGAGAAAATAGT+TGG - chr4_4:48462820-48462839 None:intergenic 25.0%
!!! TTTGTGGATTTTTATTTGAG+TGG + chr4_4:48462892-48462911 Msa0684820:three_prime_UTR 25.0%
!!! TCAGTTTTTTTTTTGTGCTT+TGG + chr4_4:48462936-48462955 Msa0684820:three_prime_UTR 25.0%
!!! TTTTTTTGTGCTTTGGATTT+TGG + chr4_4:48462943-48462962 Msa0684820:three_prime_UTR 25.0%
!!! ATGTGTCCAATTTTTTTAAC+TGG - chr4_4:48462049-48462068 None:intergenic 25.0%
! AGTATAGATATTTGATGTGT+TGG - chr4_4:48462409-48462428 None:intergenic 25.0%
! GCATTAAAAATGGTTAAAGT+TGG - chr4_4:48462433-48462452 None:intergenic 25.0%
! TCTCATAAGTGCATTAAAAA+TGG - chr4_4:48462443-48462462 None:intergenic 25.0%
! ATTTATGATCCAAAAGGAAA+AGG + chr4_4:48462589-48462608 Msa0684820:CDS 25.0%
! GGTATATATGATCATACATA+TGG - chr4_4:48462747-48462766 None:intergenic 25.0%
! ATGTATGATCATATATACCT+TGG + chr4_4:48462748-48462767 Msa0684820:three_prime_UTR 25.0%
! ATCATATATACCTTGGAATA+AGG + chr4_4:48462755-48462774 Msa0684820:three_prime_UTR 25.0%
! CATCAAAATGAGAAAATAGT+TGG - chr4_4:48462820-48462839 None:intergenic 25.0%
!!! TTTGTGGATTTTTATTTGAG+TGG + chr4_4:48462892-48462911 Msa0684820:three_prime_UTR 25.0%
!!! TCAGTTTTTTTTTTGTGCTT+TGG + chr4_4:48462936-48462955 Msa0684820:three_prime_UTR 25.0%
!!! TTTTTTTGTGCTTTGGATTT+TGG + chr4_4:48462943-48462962 Msa0684820:three_prime_UTR 25.0%
! GCATTTTCAAAAAGAAAGTC+TGG + chr4_4:48461708-48461727 Msa0684820:CDS 30.0%
!! ACAAAGTGTTGATAATTCAG+AGG - chr4_4:48461748-48461767 None:intergenic 30.0%
TCTGAATTATCAACACTTTG+TGG + chr4_4:48461747-48461766 Msa0684820:CDS 30.0%
CTGAATTATCAACACTTTGT+GGG + chr4_4:48461748-48461767 Msa0684820:CDS 30.0%
TGAATTATCAACACTTTGTG+GGG + chr4_4:48461749-48461768 Msa0684820:CDS 30.0%
! ATTCTTATTATTTCTCCAGC+AGG + chr4_4:48461783-48461802 Msa0684820:CDS 30.0%
CTTCAAAATTGTTCTTGCTA+TGG - chr4_4:48461844-48461863 None:intergenic 30.0%
TTTGAAGAATGAAAACCCTT+CGG + chr4_4:48461857-48461876 Msa0684820:CDS 30.0%
TTAATTCTTCTTCTACCGAA+GGG - chr4_4:48461875-48461894 None:intergenic 30.0%
TTTAATTCTTCTTCTACCGA+AGG - chr4_4:48461876-48461895 None:intergenic 30.0%
! CACAAAAAATGATGAGCTTT+TGG + chr4_4:48461920-48461939 Msa0684820:CDS 30.0%
AAAATTGGACACATCTTTAG+TGG + chr4_4:48462055-48462074 Msa0684820:CDS 30.0%
ATAAGTAGTGTCTTAAGCAA+TGG + chr4_4:48462095-48462114 Msa0684820:CDS 30.0%
TGTACATCTTCTAATCAACT+TGG + chr4_4:48462143-48462162 Msa0684820:intron 30.0%
AATTTCATAACCGTTGTTGT+TGG - chr4_4:48462323-48462342 None:intergenic 30.0%
AACAACAACGGTTATGAAAT+TGG + chr4_4:48462322-48462341 Msa0684820:CDS 30.0%
! TCCTTTTGGATCATAAATCT+GGG - chr4_4:48462587-48462606 None:intergenic 30.0%
! TTCCTTTTGGATCATAAATC+TGG - chr4_4:48462588-48462607 None:intergenic 30.0%
GAAGGTAGAAATAAAACACA+AGG + chr4_4:48462634-48462653 Msa0684820:CDS 30.0%
CTAGCATATCATTCATCTTT+AGG - chr4_4:48462715-48462734 None:intergenic 30.0%
TTGGAATAAGGTGATATAAG+CGG + chr4_4:48462767-48462786 Msa0684820:three_prime_UTR 30.0%
TGAGAAAATAGTTGGAATCA+TGG - chr4_4:48462812-48462831 None:intergenic 30.0%
!! TGGATTTTTATTTGAGTGGT+TGG + chr4_4:48462896-48462915 Msa0684820:three_prime_UTR 30.0%
!!! CTTTGGATTTTGGTTGTATT+GGG + chr4_4:48462953-48462972 Msa0684820:three_prime_UTR 30.0%
! GCATTTTCAAAAAGAAAGTC+TGG + chr4_4:48461708-48461727 Msa0684820:CDS 30.0%
!! ACAAAGTGTTGATAATTCAG+AGG - chr4_4:48461748-48461767 None:intergenic 30.0%
TCTGAATTATCAACACTTTG+TGG + chr4_4:48461747-48461766 Msa0684820:CDS 30.0%
CTGAATTATCAACACTTTGT+GGG + chr4_4:48461748-48461767 Msa0684820:CDS 30.0%
TGAATTATCAACACTTTGTG+GGG + chr4_4:48461749-48461768 Msa0684820:CDS 30.0%
! ATTCTTATTATTTCTCCAGC+AGG + chr4_4:48461783-48461802 Msa0684820:CDS 30.0%
CTTCAAAATTGTTCTTGCTA+TGG - chr4_4:48461844-48461863 None:intergenic 30.0%
TTTGAAGAATGAAAACCCTT+CGG + chr4_4:48461857-48461876 Msa0684820:CDS 30.0%
TTAATTCTTCTTCTACCGAA+GGG - chr4_4:48461875-48461894 None:intergenic 30.0%
TTTAATTCTTCTTCTACCGA+AGG - chr4_4:48461876-48461895 None:intergenic 30.0%
! CACAAAAAATGATGAGCTTT+TGG + chr4_4:48461920-48461939 Msa0684820:CDS 30.0%
AAAATTGGACACATCTTTAG+TGG + chr4_4:48462055-48462074 Msa0684820:CDS 30.0%
ATAAGTAGTGTCTTAAGCAA+TGG + chr4_4:48462095-48462114 Msa0684820:CDS 30.0%
TGTACATCTTCTAATCAACT+TGG + chr4_4:48462143-48462162 Msa0684820:intron 30.0%
AATTTCATAACCGTTGTTGT+TGG - chr4_4:48462323-48462342 None:intergenic 30.0%
AACAACAACGGTTATGAAAT+TGG + chr4_4:48462322-48462341 Msa0684820:CDS 30.0%
! TCCTTTTGGATCATAAATCT+GGG - chr4_4:48462587-48462606 None:intergenic 30.0%
! TTCCTTTTGGATCATAAATC+TGG - chr4_4:48462588-48462607 None:intergenic 30.0%
GAAGGTAGAAATAAAACACA+AGG + chr4_4:48462634-48462653 Msa0684820:CDS 30.0%
CTAGCATATCATTCATCTTT+AGG - chr4_4:48462715-48462734 None:intergenic 30.0%
TTGGAATAAGGTGATATAAG+CGG + chr4_4:48462767-48462786 Msa0684820:three_prime_UTR 30.0%
TGAGAAAATAGTTGGAATCA+TGG - chr4_4:48462812-48462831 None:intergenic 30.0%
!! TGGATTTTTATTTGAGTGGT+TGG + chr4_4:48462896-48462915 Msa0684820:three_prime_UTR 30.0%
!!! CTTTGGATTTTGGTTGTATT+GGG + chr4_4:48462953-48462972 Msa0684820:three_prime_UTR 30.0%
! TAACAATATGGTTTTCCTGC+TGG - chr4_4:48461801-48461820 None:intergenic 35.0%
GCAGGAAAACCATATTGTTA+TGG + chr4_4:48461801-48461820 Msa0684820:CDS 35.0%
GAAGGTTCTCCATAACAATA+TGG - chr4_4:48461813-48461832 None:intergenic 35.0%
! TTTGGATGAAAAGCATGTTG+CGG + chr4_4:48461938-48461957 Msa0684820:CDS 35.0%
!! TTGAAGGCTTTGGAAAGTTA+TGG + chr4_4:48461978-48461997 Msa0684820:CDS 35.0%
! CTTTGGAAAGTTATGGCTAT+TGG + chr4_4:48461985-48462004 Msa0684820:CDS 35.0%
TCTTGCATAGATGAACTGAA+TGG + chr4_4:48462265-48462284 Msa0684820:CDS 35.0%
ACAAATCCTCCTTATGTCTT+TGG + chr4_4:48462295-48462314 Msa0684820:CDS 35.0%
CAAATCCTCCTTATGTCTTT+GGG + chr4_4:48462296-48462315 Msa0684820:CDS 35.0%
AAATCCTCCTTATGTCTTTG+GGG + chr4_4:48462297-48462316 Msa0684820:CDS 35.0%
TATGTGTTAGGTTTATCGCA+TGG - chr4_4:48462531-48462550 None:intergenic 35.0%
TTTCTTGTCATTGAAGCTCA+TGG + chr4_4:48462559-48462578 Msa0684820:intron 35.0%
TCCCAGATTTATGATCCAAA+AGG + chr4_4:48462583-48462602 Msa0684820:CDS 35.0%
GATGTTATTGCTTCTCTTGA+AGG + chr4_4:48462616-48462635 Msa0684820:CDS 35.0%
GCTTATATCACCTTATTCCA+AGG - chr4_4:48462768-48462787 None:intergenic 35.0%
!!! GCTTTGGATTTTGGTTGTAT+TGG + chr4_4:48462952-48462971 Msa0684820:three_prime_UTR 35.0%
! TAACAATATGGTTTTCCTGC+TGG - chr4_4:48461801-48461820 None:intergenic 35.0%
GCAGGAAAACCATATTGTTA+TGG + chr4_4:48461801-48461820 Msa0684820:CDS 35.0%
GAAGGTTCTCCATAACAATA+TGG - chr4_4:48461813-48461832 None:intergenic 35.0%
! TTTGGATGAAAAGCATGTTG+CGG + chr4_4:48461938-48461957 Msa0684820:CDS 35.0%
!! TTGAAGGCTTTGGAAAGTTA+TGG + chr4_4:48461978-48461997 Msa0684820:CDS 35.0%
! CTTTGGAAAGTTATGGCTAT+TGG + chr4_4:48461985-48462004 Msa0684820:CDS 35.0%
TCTTGCATAGATGAACTGAA+TGG + chr4_4:48462265-48462284 Msa0684820:CDS 35.0%
ACAAATCCTCCTTATGTCTT+TGG + chr4_4:48462295-48462314 Msa0684820:CDS 35.0%
CAAATCCTCCTTATGTCTTT+GGG + chr4_4:48462296-48462315 Msa0684820:CDS 35.0%
AAATCCTCCTTATGTCTTTG+GGG + chr4_4:48462297-48462316 Msa0684820:CDS 35.0%
TATGTGTTAGGTTTATCGCA+TGG - chr4_4:48462531-48462550 None:intergenic 35.0%
TTTCTTGTCATTGAAGCTCA+TGG + chr4_4:48462559-48462578 Msa0684820:intron 35.0%
TCCCAGATTTATGATCCAAA+AGG + chr4_4:48462583-48462602 Msa0684820:CDS 35.0%
GATGTTATTGCTTCTCTTGA+AGG + chr4_4:48462616-48462635 Msa0684820:CDS 35.0%
GCTTATATCACCTTATTCCA+AGG - chr4_4:48462768-48462787 None:intergenic 35.0%
!!! GCTTTGGATTTTGGTTGTAT+TGG + chr4_4:48462952-48462971 Msa0684820:three_prime_UTR 35.0%
! CTTGCTATGGATTGAGAAGA+AGG - chr4_4:48461831-48461850 None:intergenic 40.0%
AGCTCATCAGGAAAGTTTGA+AGG + chr4_4:48461962-48461981 Msa0684820:CDS 40.0%
TCAGGAAAGTTTGAAGGCTT+TGG + chr4_4:48461968-48461987 Msa0684820:CDS 40.0%
AGTAGTGTCTTAAGCAATGG+AGG + chr4_4:48462098-48462117 Msa0684820:CDS 40.0%
AGACATAAGGAGGATTTGTC+TGG - chr4_4:48462294-48462313 None:intergenic 40.0%
TGATCCAAAAGGAAAAGGCA+AGG + chr4_4:48462594-48462613 Msa0684820:CDS 40.0%
! ACATCCTTGCCTTTTCCTTT+TGG - chr4_4:48462601-48462620 None:intergenic 40.0%
CATTTCCACTTTCACTTGCA+CGG - chr4_4:48462672-48462691 None:intergenic 40.0%
ATAAGCGGACATCACATTTC+AGG + chr4_4:48462782-48462801 Msa0684820:three_prime_UTR 40.0%
TAAGCGGACATCACATTTCA+GGG + chr4_4:48462783-48462802 Msa0684820:three_prime_UTR 40.0%
! CTTGCTATGGATTGAGAAGA+AGG - chr4_4:48461831-48461850 None:intergenic 40.0%
AGCTCATCAGGAAAGTTTGA+AGG + chr4_4:48461962-48461981 Msa0684820:CDS 40.0%
TCAGGAAAGTTTGAAGGCTT+TGG + chr4_4:48461968-48461987 Msa0684820:CDS 40.0%
AGTAGTGTCTTAAGCAATGG+AGG + chr4_4:48462098-48462117 Msa0684820:CDS 40.0%
AGACATAAGGAGGATTTGTC+TGG - chr4_4:48462294-48462313 None:intergenic 40.0%
TGATCCAAAAGGAAAAGGCA+AGG + chr4_4:48462594-48462613 Msa0684820:CDS 40.0%
! ACATCCTTGCCTTTTCCTTT+TGG - chr4_4:48462601-48462620 None:intergenic 40.0%
CATTTCCACTTTCACTTGCA+CGG - chr4_4:48462672-48462691 None:intergenic 40.0%
ATAAGCGGACATCACATTTC+AGG + chr4_4:48462782-48462801 Msa0684820:three_prime_UTR 40.0%
TAAGCGGACATCACATTTCA+GGG + chr4_4:48462783-48462802 Msa0684820:three_prime_UTR 40.0%
CTACCTCTTGATGTCCTCTT+CGG - chr4_4:48461639-48461658 None:intergenic 45.0%
GGAGGAGAGATTAATCCTCA+TGG + chr4_4:48462116-48462135 Msa0684820:CDS 45.0%
GAAGATGTACACGTACCATG+AGG - chr4_4:48462134-48462153 None:intergenic 45.0%
TTGTTGGCCCCAAAGACATA+AGG - chr4_4:48462307-48462326 None:intergenic 45.0%
CGTGCAAGTGAAAGTGGAAA+TGG + chr4_4:48462670-48462689 Msa0684820:CDS 45.0%
CTACCTCTTGATGTCCTCTT+CGG - chr4_4:48461639-48461658 None:intergenic 45.0%
GGAGGAGAGATTAATCCTCA+TGG + chr4_4:48462116-48462135 Msa0684820:CDS 45.0%
GAAGATGTACACGTACCATG+AGG - chr4_4:48462134-48462153 None:intergenic 45.0%
TTGTTGGCCCCAAAGACATA+AGG - chr4_4:48462307-48462326 None:intergenic 45.0%
CGTGCAAGTGAAAGTGGAAA+TGG + chr4_4:48462670-48462689 Msa0684820:CDS 45.0%
TTGGCCCCAAAGACATAAGG+AGG - chr4_4:48462304-48462323 None:intergenic 50.0%
!! GTCTTTGGGGCCAACAACAA+CGG + chr4_4:48462310-48462329 Msa0684820:CDS 50.0%
TCACTTGCACGGTCAAGGTT+TGG - chr4_4:48462661-48462680 None:intergenic 50.0%
CACTTTCACTTGCACGGTCA+AGG - chr4_4:48462666-48462685 None:intergenic 50.0%
CTTGACCGTGCAAGTGAAAG+TGG + chr4_4:48462664-48462683 Msa0684820:CDS 50.0%
TTGGCCCCAAAGACATAAGG+AGG - chr4_4:48462304-48462323 None:intergenic 50.0%
!! GTCTTTGGGGCCAACAACAA+CGG + chr4_4:48462310-48462329 Msa0684820:CDS 50.0%
TCACTTGCACGGTCAAGGTT+TGG - chr4_4:48462661-48462680 None:intergenic 50.0%
CACTTTCACTTGCACGGTCA+AGG - chr4_4:48462666-48462685 None:intergenic 50.0%
CTTGACCGTGCAAGTGAAAG+TGG + chr4_4:48462664-48462683 Msa0684820:CDS 50.0%
GCTCCGAAGAGGACATCAAG+AGG + chr4_4:48461633-48461652 Msa0684820:CDS 55.0%
GCATGTTGCGGAAGCTCATC+AGG + chr4_4:48461950-48461969 Msa0684820:CDS 55.0%
GCGGACATCACATTTCAGGG+TGG + chr4_4:48462786-48462805 Msa0684820:three_prime_UTR 55.0%
GCTCCGAAGAGGACATCAAG+AGG + chr4_4:48461633-48461652 Msa0684820:CDS 55.0%
GCATGTTGCGGAAGCTCATC+AGG + chr4_4:48461950-48461969 Msa0684820:CDS 55.0%
GCGGACATCACATTTCAGGG+TGG + chr4_4:48462786-48462805 Msa0684820:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 48461630 48462976 48461630 ID=Msa0684820;Name=Msa0684820
chr4_4 mRNA 48461630 48462976 48461630 ID=Msa0684820-mRNA-1;Parent=Msa0684820;Name=Msa0684820-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0.5|0.33|1|1|1|3|250|249
chr4_4 exon 48461630 48462137 48461630 ID=Msa0684820-mRNA-1:exon:11980;Parent=Msa0684820-mRNA-1
chr4_4 exon 48462260 48462349 48462260 ID=Msa0684820-mRNA-1:exon:11981;Parent=Msa0684820-mRNA-1
chr4_4 exon 48462575 48462976 48462575 ID=Msa0684820-mRNA-1:exon:11982;Parent=Msa0684820-mRNA-1
chr4_4 CDS 48461630 48462137 48461630 ID=Msa0684820-mRNA-1:cds;Parent=Msa0684820-mRNA-1
chr4_4 CDS 48462260 48462349 48462260 ID=Msa0684820-mRNA-1:cds;Parent=Msa0684820-mRNA-1
chr4_4 CDS 48462575 48462726 48462575 ID=Msa0684820-mRNA-1:cds;Parent=Msa0684820-mRNA-1
chr4_4 three_prime_UTR 48462727 48462976 48462727 ID=Msa0684820-mRNA-1:three_prime_utr;Parent=Msa0684820-mRNA-1
Gene Sequence

>Msa0684820

ATGGCTCCGAAGAGGACATCAAGAGGTAGACAAAAAAGAGAAATGAAGAAGATTGAGAGAGAATGCGATCTTTTAGTAGCATTTTCAAAAAGAAAGTCTGGCATTTACAACAAAGCCTCTGAATTATCAACACTTTGTGGGGCTTTAGTTGATATTCTTATTATTTCTCCAGCAGGAAAACCATATTGTTATGGAGAACCTTCTTCTCAATCCATAGCAAGAACAATTTTGAAGAATGAAAACCCTTCGGTAGAAGAAGAATTAAAGCAACTAAAGATTAACAAAGTTAACACAAAAAATGATGAGCTTTTGGATGAAAAGCATGTTGCGGAAGCTCATCAGGAAAGTTTGAAGGCTTTGGAAAGTTATGGCTATTGGTCGATCAATGAGAAAGATGTTGAAAGCTTTGATTATGACCAGTTAAAAAAATTGGACACATCTTTAGTGGACATTGCAAATAAATTGATAAGTAGTGTCTTAAGCAATGGAGGAGAGATTAATCCTCATGCAATTTCTTGCATAGATGAACTGAATGGAGTCCAGACAAATCCTCCTTATGTCTTTGGGGCCAACAACAACGGTTATGAAATTGGTGCAACTCATGGATCCCAGATTTATGATCCAAAAGGAAAAGGCAAGGATGTTATTGCTTCTCTTGAAGGTAGAAATAAAACACAAGGACCAAACCTTGACCGTGCAAGTGAAAGTGGAAATGGAAGTGATGTTGATCATTCTCCTAAAGATGAATGA
Protein sequence

>Msa0684820

MAPKRTSRGRQKREMKKIERECDLLVAFSKRKSGIYNKASELSTLCGALVDILIISPAGKPYCYGEPSSQSIARTILKNENPSVEEELKQLKINKVNTKNDELLDEKHVAEAHQESLKALESYGYWSINEKDVESFDYDQLKKLDTSLVDIANKLISSVLSNGGEINPHAISCIDELNGVQTNPPYVFGANNNGYEIGATHGSQIYDPKGKGKDVIASLEGRNKTQGPNLDRASESGNGSDVDHSPKDE*