Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0687850 | XP_039687084.1 | 85.246 | 122 | 18 | 0 | 1 | 122 | 1 | 122 | 4.37e-71 | 218 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0687850 | G7INX6 | 85.246 | 122 | 18 | 0 | 1 | 122 | 1 | 122 | 2.09e-71 | 218 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0687850 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0687850 | MtrunA17_Chr2g0281511 | 85.246 | 122 | 18 | 0 | 1 | 122 | 1 | 122 | 4.02e-75 | 218 |
| Msa0687850 | MtrunA17_Chr2g0309121 | 81.967 | 122 | 21 | 1 | 1 | 122 | 1 | 121 | 7.39e-70 | 205 |
| Msa0687850 | MtrunA17_Chr3g0081891 | 84.810 | 79 | 12 | 0 | 1 | 79 | 1 | 79 | 7.79e-45 | 140 |
| Msa0687850 | MtrunA17_Chr7g0276661 | 35.115 | 131 | 65 | 4 | 7 | 119 | 38 | 166 | 3.86e-21 | 83.2 |
| Msa0687850 | MtrunA17_Chr0c01g0489371 | 41.758 | 91 | 51 | 1 | 22 | 110 | 224 | 314 | 2.78e-17 | 75.9 |
| Msa0687850 | MtrunA17_Chr8g0356961 | 40.449 | 89 | 51 | 1 | 24 | 110 | 77 | 165 | 1.10e-16 | 71.6 |
| Msa0687850 | MtrunA17_Chr8g0372431 | 29.897 | 97 | 68 | 0 | 24 | 120 | 6 | 102 | 1.25e-16 | 69.7 |
| Msa0687850 | MtrunA17_Chr6g0485871 | 33.721 | 86 | 56 | 1 | 24 | 108 | 214 | 299 | 8.65e-13 | 63.2 |
| Msa0687850 | MtrunA17_Chr8g0356951 | 37.363 | 91 | 53 | 2 | 24 | 110 | 217 | 307 | 1.21e-12 | 62.8 |
| Msa0687850 | MtrunA17_Chr5g0434641 | 34.066 | 91 | 58 | 1 | 22 | 110 | 208 | 298 | 1.61e-12 | 62.4 |
| Msa0687850 | MtrunA17_Chr7g0228081 | 31.250 | 80 | 55 | 0 | 41 | 120 | 13 | 92 | 4.05e-12 | 57.8 |
| Msa0687850 | MtrunA17_Chr5g0430431 | 42.623 | 61 | 35 | 0 | 56 | 116 | 194 | 254 | 8.33e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATGTTACTGCTTCTATTT+TGG | 0.123735 | 4_4:+52841107 | Msa0687850:CDS |
| AAAATTCCGATGTTCATTAT+TGG | 0.199581 | 4_4:+52841079 | Msa0687850:CDS |
| GAAGTCAATGGTGCTGAATT+AGG | 0.200131 | 4_4:+52841048 | Msa0687850:CDS |
| TACCAGTTTCTTCATCAAAA+AGG | 0.252196 | 4_4:-52841298 | None:intergenic |
| TGTATGCTACCGTCGTTAAC+TGG | 0.297739 | 4_4:+52841465 | Msa0687850:CDS |
| CCTCGACGTGTTGCCGAGAA+TGG | 0.307864 | 4_4:+52841248 | Msa0687850:CDS |
| CGGTCAATAATCCTCCTAAT+AGG | 0.355854 | 4_4:+52841441 | Msa0687850:CDS |
| TTTGGATGGAAACAATGTTT+TGG | 0.356448 | 4_4:+52841125 | Msa0687850:CDS |
| AAGTCAATGGTGCTGAATTA+GGG | 0.416476 | 4_4:+52841049 | Msa0687850:CDS |
| ACGGTAGCATACATCCTATT+AGG | 0.440754 | 4_4:-52841455 | None:intergenic |
| ATTGGTATACAAACCATTCT+CGG | 0.470714 | 4_4:-52841261 | None:intergenic |
| GTATGCTACCGTCGTTAACT+GGG | 0.522932 | 4_4:+52841466 | Msa0687850:CDS |
| GCATGGATCAGAAATACATA+GGG | 0.559318 | 4_4:+52841357 | Msa0687850:CDS |
| AGCATGGATCAGAAATACAT+AGG | 0.564126 | 4_4:+52841356 | Msa0687850:CDS |
| CTTGAAAAGTGCCTAAAAGT+TGG | 0.570138 | 4_4:+52841401 | Msa0687850:CDS |
| AGAAATACATAGGGAGAGGA+TGG | 0.573439 | 4_4:+52841366 | Msa0687850:CDS |
| GATCAGAAATACATAGGGAG+AGG | 0.587262 | 4_4:+52841362 | Msa0687850:CDS |
| TCACACTGCGTCAAGAAGCA+TGG | 0.587686 | 4_4:+52841340 | Msa0687850:CDS |
| CCATTCTCGGCAACACGTCG+AGG | 0.599354 | 4_4:-52841248 | None:intergenic |
| GAGATTGGAGTTGAAGTCAA+TGG | 0.619642 | 4_4:+52841036 | Msa0687850:CDS |
| GTAGCATACATCCTATTAGG+AGG | 0.633251 | 4_4:-52841452 | None:intergenic |
| ATACTTCCAATAATGAACAT+CGG | 0.646998 | 4_4:-52841085 | None:intergenic |
| TAATCAGTCCCAGTTAACGA+CGG | 0.733495 | 4_4:-52841474 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATTCCGATGTTCATTAT+TGG | + | chr4_4:52841079-52841098 | Msa0687850:CDS | 25.0% |
| ! | ATACTTCCAATAATGAACAT+CGG | - | chr4_4:52841088-52841107 | None:intergenic | 25.0% |
| !! | TAATGTTACTGCTTCTATTT+TGG | + | chr4_4:52841107-52841126 | Msa0687850:CDS | 25.0% |
| !! | AAGGAGAATTTTAGATTGAT+TGG | - | chr4_4:52841282-52841301 | None:intergenic | 25.0% |
| ! | TTTGGATGGAAACAATGTTT+TGG | + | chr4_4:52841125-52841144 | Msa0687850:CDS | 30.0% |
| ATTGGTATACAAACCATTCT+CGG | - | chr4_4:52841264-52841283 | None:intergenic | 30.0% | |
| TACCAGTTTCTTCATCAAAA+AGG | - | chr4_4:52841301-52841320 | None:intergenic | 30.0% | |
| ! | AAACAACATCACCAACTTTT+AGG | - | chr4_4:52841415-52841434 | None:intergenic | 30.0% |
| ! | AAGTCAATGGTGCTGAATTA+GGG | + | chr4_4:52841049-52841068 | Msa0687850:CDS | 35.0% |
| !! | GTTACTGCTTCTATTTTGGA+TGG | + | chr4_4:52841111-52841130 | Msa0687850:CDS | 35.0% |
| !!! | GGAAACAATGTTTTGGTTAG+TGG | + | chr4_4:52841132-52841151 | Msa0687850:intron | 35.0% |
| ! | CTCCTTTTTGATGAAGAAAC+TGG | + | chr4_4:52841296-52841315 | Msa0687850:CDS | 35.0% |
| AGCATGGATCAGAAATACAT+AGG | + | chr4_4:52841356-52841375 | Msa0687850:CDS | 35.0% | |
| GCATGGATCAGAAATACATA+GGG | + | chr4_4:52841357-52841376 | Msa0687850:CDS | 35.0% | |
| !! | CTTGAAAAGTGCCTAAAAGT+TGG | + | chr4_4:52841401-52841420 | Msa0687850:CDS | 35.0% |
| !!! | TGGTGATGTTGTTTTGTTCA+CGG | + | chr4_4:52841421-52841440 | Msa0687850:CDS | 35.0% |
| GAGATTGGAGTTGAAGTCAA+TGG | + | chr4_4:52841036-52841055 | Msa0687850:CDS | 40.0% | |
| ! | GAAGTCAATGGTGCTGAATT+AGG | + | chr4_4:52841048-52841067 | Msa0687850:CDS | 40.0% |
| GATCAGAAATACATAGGGAG+AGG | + | chr4_4:52841362-52841381 | Msa0687850:CDS | 40.0% | |
| AGAAATACATAGGGAGAGGA+TGG | + | chr4_4:52841366-52841385 | Msa0687850:CDS | 40.0% | |
| CGGTCAATAATCCTCCTAAT+AGG | + | chr4_4:52841441-52841460 | Msa0687850:CDS | 40.0% | |
| GTAGCATACATCCTATTAGG+AGG | - | chr4_4:52841455-52841474 | None:intergenic | 40.0% | |
| ACGGTAGCATACATCCTATT+AGG | - | chr4_4:52841458-52841477 | None:intergenic | 40.0% | |
| TGTATGCTACCGTCGTTAAC+TGG | + | chr4_4:52841465-52841484 | Msa0687850:CDS | 45.0% | |
| GTATGCTACCGTCGTTAACT+GGG | + | chr4_4:52841466-52841485 | Msa0687850:CDS | 45.0% | |
| TCACACTGCGTCAAGAAGCA+TGG | + | chr4_4:52841340-52841359 | Msa0687850:CDS | 50.0% | |
| CCATTCTCGGCAACACGTCG+AGG | - | chr4_4:52841251-52841270 | None:intergenic | 60.0% | |
| CCTCGACGTGTTGCCGAGAA+TGG | + | chr4_4:52841248-52841267 | Msa0687850:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 52841030 | 52841493 | 52841030 | ID=Msa0687850;Name=Msa0687850 |
| chr4_4 | mRNA | 52841030 | 52841493 | 52841030 | ID=Msa0687850-mRNA-1;Parent=Msa0687850;Name=Msa0687850-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|1|1|2|0|122 |
| chr4_4 | exon | 52841030 | 52841146 | 52841030 | ID=Msa0687850-mRNA-1:exon:13934;Parent=Msa0687850-mRNA-1 |
| chr4_4 | exon | 52841242 | 52841493 | 52841242 | ID=Msa0687850-mRNA-1:exon:13935;Parent=Msa0687850-mRNA-1 |
| chr4_4 | CDS | 52841030 | 52841146 | 52841030 | ID=Msa0687850-mRNA-1:cds;Parent=Msa0687850-mRNA-1 |
| chr4_4 | CDS | 52841242 | 52841493 | 52841242 | ID=Msa0687850-mRNA-1:cds;Parent=Msa0687850-mRNA-1 |
| Gene Sequence |
| Protein sequence |
