Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0689700 | AES78040.1 | 90.164 | 183 | 7 | 1 | 1 | 172 | 1 | 183 | 4.89e-114 | 331 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0689700 | sp|O81788|WOX13_ARATH | 54.237 | 118 | 39 | 1 | 17 | 119 | 33 | 150 | 7.14e-34 | 123 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0689700 | G7KVS3 | 90.164 | 183 | 7 | 1 | 1 | 172 | 1 | 183 | 2.33e-114 | 331 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0689700 | TF | HB-WOX |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0689700 | MtrunA17_Chr7g0222981 | 90.164 | 183 | 7 | 1 | 1 | 172 | 1 | 183 | 4.49e-118 | 331 |
| Msa0689700 | MtrunA17_Chr1g0212511 | 49.315 | 219 | 63 | 8 | 1 | 172 | 5 | 222 | 3.04e-53 | 170 |
| Msa0689700 | MtrunA17_Chr3g0144021 | 38.220 | 191 | 81 | 5 | 9 | 165 | 7 | 194 | 3.75e-32 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0689700 | AT4G35550.1 | 54.237 | 118 | 39 | 1 | 17 | 119 | 33 | 150 | 7.26e-35 | 123 |
| Msa0689700 | AT1G20700.3 | 43.089 | 123 | 52 | 3 | 15 | 119 | 24 | 146 | 3.00e-24 | 94.4 |
| Msa0689700 | AT1G20700.1 | 43.089 | 123 | 52 | 3 | 15 | 119 | 24 | 146 | 3.55e-24 | 94.4 |
| Msa0689700 | AT1G20710.1 | 39.837 | 123 | 56 | 1 | 15 | 119 | 8 | 130 | 7.94e-15 | 69.3 |
| Msa0689700 | AT1G20700.2 | 50.000 | 54 | 27 | 0 | 66 | 119 | 31 | 84 | 9.39e-12 | 60.1 |
Find 30 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACTTGCATCATTCCAATAT+TGG | 0.240745 | 4_4:-56864516 | None:intergenic |
| TTTAATCAGAACGCCAATAT+TGG | 0.268262 | 4_4:+56864503 | Msa0689700:CDS |
| ACCTGTGCAACTGCAAAGTT+TGG | 0.336692 | 4_4:+56864883 | Msa0689700:CDS |
| GAGATCTTGTTGAGAAGATA+AGG | 0.380614 | 4_4:-56864630 | None:intergenic |
| TCCTAGTAAGGAAAAGATAA+AGG | 0.397905 | 4_4:+56864934 | Msa0689700:CDS |
| TACACACTGGTCTCTGAAAT+CGG | 0.399480 | 4_4:-56864986 | None:intergenic |
| TTTGTGCAGCCAGTACTTAG+AGG | 0.411680 | 4_4:+56865149 | Msa0689700:CDS |
| CTCTGAAATCGGCCCGTGCT+TGG | 0.426359 | 4_4:-56864975 | None:intergenic |
| TCAACCTCAGTCTCTACTTC+CGG | 0.438371 | 4_4:-56865072 | None:intergenic |
| AAATAATGTTAATGATGAAC+CGG | 0.459463 | 4_4:+56865053 | Msa0689700:CDS |
| GACTGAGATGCAACGATCTC+CGG | 0.461176 | 4_4:-56865117 | None:intergenic |
| CGAATATTTGAAGCTGAGAC+GGG | 0.462716 | 4_4:+56864908 | Msa0689700:CDS |
| ATGAACAAGTCGAAACTCTT+CGG | 0.463263 | 4_4:+56864552 | Msa0689700:CDS |
| TCGTAAACTGTCATCTGATT+CGG | 0.469903 | 4_4:-56865181 | None:intergenic |
| TCCAAACTTTGCAGTTGCAC+AGG | 0.484501 | 4_4:-56864884 | None:intergenic |
| CTACTAGGCTGAGGCAGCGT+TGG | 0.489931 | 4_4:+56864852 | Msa0689700:CDS |
| CAATGATAAGAAAACTGAAC+CGG | 0.495461 | 4_4:+56865098 | Msa0689700:CDS |
| AAGAGTTTCGACTTGTTCAT+CGG | 0.503160 | 4_4:-56864549 | None:intergenic |
| AATTCAAGTCCTCTAAGTAC+TGG | 0.519200 | 4_4:-56865158 | None:intergenic |
| GCGAATATTTGAAGCTGAGA+CGG | 0.525730 | 4_4:+56864907 | Msa0689700:CDS |
| TGAGACGGGGACTCCTAGTA+AGG | 0.526200 | 4_4:+56864922 | Msa0689700:CDS |
| CTTTGCAGTTGCACAGGTGT+TGG | 0.529341 | 4_4:-56864878 | None:intergenic |
| CAACAAGATCTCACTCTCAC+TGG | 0.535564 | 4_4:+56864641 | Msa0689700:CDS |
| ACCGCTGATTTAACCAAGCA+CGG | 0.547494 | 4_4:+56864962 | Msa0689700:CDS |
| GAATAGGCGTGCTAGGTCAA+AGG | 0.572061 | 4_4:+56865021 | Msa0689700:intron |
| CCCGTGCTTGGTTAAATCAG+CGG | 0.597671 | 4_4:-56864963 | None:intergenic |
| GAGATGGAGTGGCAGAAGAA+CGG | 0.607848 | 4_4:+56864479 | None:intergenic |
| CCGCTGATTTAACCAAGCAC+GGG | 0.638814 | 4_4:+56864963 | Msa0689700:CDS |
| GAATATTTGAAGCTGAGACG+GGG | 0.658394 | 4_4:+56864909 | Msa0689700:CDS |
| TGAACCGGAAGTAGAGACTG+AGG | 0.698771 | 4_4:+56865068 | Msa0689700:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACATTTAATGATGATTTTT+AGG | + | chr4_4:56864827-56864846 | Msa0689700:intron | 15.0% |
| !!! | ACATTTAATGATGATTTTTA+GGG | + | chr4_4:56864828-56864847 | Msa0689700:intron | 15.0% |
| !! | AAATAATGTTAATGATGAAC+CGG | + | chr4_4:56865053-56865072 | Msa0689700:CDS | 20.0% |
| !! | TGTATAATTGGTTTCAGAAT+AGG | + | chr4_4:56865005-56865024 | Msa0689700:intron | 25.0% |
| TTTAATCAGAACGCCAATAT+TGG | + | chr4_4:56864503-56864522 | Msa0689700:CDS | 30.0% | |
| TACTTGCATCATTCCAATAT+TGG | - | chr4_4:56864519-56864538 | None:intergenic | 30.0% | |
| TCCTAGTAAGGAAAAGATAA+AGG | + | chr4_4:56864934-56864953 | Msa0689700:CDS | 30.0% | |
| ! | TCCTTTATCTTTTCCTTACT+AGG | - | chr4_4:56864938-56864957 | None:intergenic | 30.0% |
| CAATGATAAGAAAACTGAAC+CGG | + | chr4_4:56865098-56865117 | Msa0689700:CDS | 30.0% | |
| ! | AAGAGTTTCGACTTGTTCAT+CGG | - | chr4_4:56864552-56864571 | None:intergenic | 35.0% |
| ATGAACAAGTCGAAACTCTT+CGG | + | chr4_4:56864552-56864571 | Msa0689700:CDS | 35.0% | |
| GAGATCTTGTTGAGAAGATA+AGG | - | chr4_4:56864633-56864652 | None:intergenic | 35.0% | |
| CTTCAGTTACTCATTCTCAA+CGG | + | chr4_4:56864685-56864704 | Msa0689700:intron | 35.0% | |
| ! | ACCGGTTCATTATATTGAAC+CGG | - | chr4_4:56864734-56864753 | None:intergenic | 35.0% |
| TAATGAACCGGTCTTTAGTA+CGG | + | chr4_4:56864742-56864761 | Msa0689700:intron | 35.0% | |
| !!! | GATGATTTTTAGGGTCTACT+AGG | + | chr4_4:56864837-56864856 | Msa0689700:intron | 35.0% |
| CTGAAACCAATTATACACAC+TGG | - | chr4_4:56865002-56865021 | None:intergenic | 35.0% | |
| ! | CGGTTCAGTTTTCTTATCAT+TGG | - | chr4_4:56865100-56865119 | None:intergenic | 35.0% |
| AATTCAAGTCCTCTAAGTAC+TGG | - | chr4_4:56865161-56865180 | None:intergenic | 35.0% | |
| TCGTAAACTGTCATCTGATT+CGG | - | chr4_4:56865184-56865203 | None:intergenic | 35.0% | |
| TCAACGGTATCACAGTTAAC+CGG | + | chr4_4:56864701-56864720 | Msa0689700:intron | 40.0% | |
| CCGGTTCATTATATTGAACC+GGG | - | chr4_4:56864733-56864752 | None:intergenic | 40.0% | |
| CCCGGTTCAATATAATGAAC+CGG | + | chr4_4:56864730-56864749 | Msa0689700:intron | 40.0% | |
| AACCAAACCGTACTAAAGAC+CGG | - | chr4_4:56864752-56864771 | None:intergenic | 40.0% | |
| ! | GCGAATATTTGAAGCTGAGA+CGG | + | chr4_4:56864907-56864926 | Msa0689700:CDS | 40.0% |
| ! | CGAATATTTGAAGCTGAGAC+GGG | + | chr4_4:56864908-56864927 | Msa0689700:CDS | 40.0% |
| ! | GAATATTTGAAGCTGAGACG+GGG | + | chr4_4:56864909-56864928 | Msa0689700:CDS | 40.0% |
| !! | TACACACTGGTCTCTGAAAT+CGG | - | chr4_4:56864989-56865008 | None:intergenic | 40.0% |
| CAGAGACCAGTGTGTATAAT+TGG | + | chr4_4:56864993-56865012 | Msa0689700:intron | 40.0% | |
| CAACAAGATCTCACTCTCAC+TGG | + | chr4_4:56864641-56864660 | Msa0689700:CDS | 45.0% | |
| CAACGGTATCACAGTTAACC+GGG | + | chr4_4:56864702-56864721 | Msa0689700:intron | 45.0% | |
| AACCGGTCTTTAGTACGGTT+TGG | + | chr4_4:56864747-56864766 | Msa0689700:intron | 45.0% | |
| !!! | TTTTAGGGTCTACTAGGCTG+AGG | + | chr4_4:56864843-56864862 | Msa0689700:intron | 45.0% |
| ACCTGTGCAACTGCAAAGTT+TGG | + | chr4_4:56864883-56864902 | Msa0689700:CDS | 45.0% | |
| TCCAAACTTTGCAGTTGCAC+AGG | - | chr4_4:56864887-56864906 | None:intergenic | 45.0% | |
| !! | ACCGCTGATTTAACCAAGCA+CGG | + | chr4_4:56864962-56864981 | Msa0689700:CDS | 45.0% |
| TCAACCTCAGTCTCTACTTC+CGG | - | chr4_4:56865075-56865094 | None:intergenic | 45.0% | |
| TTTGTGCAGCCAGTACTTAG+AGG | + | chr4_4:56865149-56865168 | Msa0689700:CDS | 45.0% | |
| CTTTGCAGTTGCACAGGTGT+TGG | - | chr4_4:56864881-56864900 | None:intergenic | 50.0% | |
| CCCGTGCTTGGTTAAATCAG+CGG | - | chr4_4:56864966-56864985 | None:intergenic | 50.0% | |
| !! | CCGCTGATTTAACCAAGCAC+GGG | + | chr4_4:56864963-56864982 | Msa0689700:CDS | 50.0% |
| GGTTTCAGAATAGGCGTGCT+AGG | + | chr4_4:56865014-56865033 | Msa0689700:intron | 50.0% | |
| GAATAGGCGTGCTAGGTCAA+AGG | + | chr4_4:56865021-56865040 | Msa0689700:intron | 50.0% | |
| TGAACCGGAAGTAGAGACTG+AGG | + | chr4_4:56865068-56865087 | Msa0689700:CDS | 50.0% | |
| GACTGAGATGCAACGATCTC+CGG | - | chr4_4:56865120-56865139 | None:intergenic | 50.0% | |
| CGGTATCACAGTTAACCGGG+TGG | + | chr4_4:56864705-56864724 | Msa0689700:intron | 55.0% | |
| ATATTGAACCGGGCTCCACC+CGG | - | chr4_4:56864723-56864742 | None:intergenic | 55.0% | |
| TGAGACGGGGACTCCTAGTA+AGG | + | chr4_4:56864922-56864941 | Msa0689700:CDS | 55.0% | |
| ACAGTTAACCGGGTGGAGCC+CGG | + | chr4_4:56864712-56864731 | Msa0689700:intron | 60.0% | |
| CTACTAGGCTGAGGCAGCGT+TGG | + | chr4_4:56864852-56864871 | Msa0689700:CDS | 60.0% | |
| !! | CTCTGAAATCGGCCCGTGCT+TGG | - | chr4_4:56864978-56864997 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 56864482 | 56865218 | 56864482 | ID=Msa0689700;Name=Msa0689700 |
| chr4_4 | mRNA | 56864482 | 56865218 | 56864482 | ID=Msa0689700-mRNA-1;Parent=Msa0689700;Name=Msa0689700-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0|1|0.5|0.33|3|0|172 |
| chr4_4 | exon | 56864482 | 56864662 | 56864482 | ID=Msa0689700-mRNA-1:exon:15074;Parent=Msa0689700-mRNA-1 |
| chr4_4 | exon | 56864849 | 56865003 | 56864849 | ID=Msa0689700-mRNA-1:exon:15075;Parent=Msa0689700-mRNA-1 |
| chr4_4 | exon | 56865036 | 56865218 | 56865036 | ID=Msa0689700-mRNA-1:exon:15076;Parent=Msa0689700-mRNA-1 |
| chr4_4 | CDS | 56864482 | 56864662 | 56864482 | ID=Msa0689700-mRNA-1:cds;Parent=Msa0689700-mRNA-1 |
| chr4_4 | CDS | 56864849 | 56865003 | 56864849 | ID=Msa0689700-mRNA-1:cds;Parent=Msa0689700-mRNA-1 |
| chr4_4 | CDS | 56865036 | 56865218 | 56865036 | ID=Msa0689700-mRNA-1:cds;Parent=Msa0689700-mRNA-1 |
| Gene Sequence |
| Protein sequence |
