AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0689920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0689920 MtrunA17_Chr1g0198651 97.251 291 8 0 1 291 1 291 0.0 598
Msa0689920 MtrunA17_Chr5g0422571 62.633 281 90 5 1 275 1 272 1.54e-113 329
Msa0689920 MtrunA17_Chr1g0173841 65.094 212 60 2 75 286 188 385 1.91e-99 297
Msa0689920 MtrunA17_Chr7g0258011 71.809 188 39 2 99 286 173 346 2.03e-99 295
Msa0689920 MtrunA17_Chr2g0328921 71.429 189 40 3 99 286 135 310 1.76e-96 286
Msa0689920 MtrunA17_Chr8g0352011 69.730 185 42 2 99 283 163 333 2.74e-96 287
Msa0689920 MtrunA17_Chr2g0321291 69.841 189 41 4 99 284 130 305 7.18e-96 285
Msa0689920 MtrunA17_Chr4g0026521 68.254 189 45 4 99 284 115 291 1.96e-95 283
Msa0689920 MtrunA17_Chr4g0053341 68.391 174 50 2 87 260 61 229 1.18e-86 259
Msa0689920 MtrunA17_Chr1g0200421 41.447 152 83 6 107 255 244 392 5.71e-27 109
Msa0689920 MtrunA17_Chr2g0320341 38.415 164 92 8 105 263 235 394 1.97e-23 99.0
Msa0689920 MtrunA17_Chr1g0181381 31.081 222 135 7 60 276 8 216 2.23e-21 94.0
Msa0689920 MtrunA17_Chr3g0123941 35.000 180 97 8 117 283 139 311 3.56e-21 91.7
Msa0689920 MtrunA17_Chr7g0263941 35.862 145 78 5 107 249 55 186 4.36e-21 92.4
Msa0689920 MtrunA17_Chr8g0382121 35.542 166 99 6 117 279 127 287 7.86e-21 90.5
Msa0689920 MtrunA17_Chr1g0163001 31.188 202 124 5 50 249 7 195 1.02e-20 92.0
Msa0689920 MtrunA17_Chr1g0210821 35.862 145 78 5 107 249 65 196 1.50e-20 91.7
Msa0689920 MtrunA17_Chr4g0029521 34.507 142 81 4 109 249 61 191 1.71e-20 91.3
Msa0689920 MtrunA17_Chr1g0185121 34.667 150 84 5 107 254 61 198 3.04e-20 90.1
Msa0689920 MtrunA17_Chr1g0199101 35.172 145 79 5 107 249 68 199 3.49e-20 90.5
Msa0689920 MtrunA17_Chr8g0342421 33.333 156 89 6 109 262 85 227 4.69e-20 90.1
Msa0689920 MtrunA17_Chr1g0162971 37.063 143 75 5 109 249 64 193 5.18e-20 89.7
Msa0689920 MtrunA17_Chr1g0151351 34.161 161 91 6 91 249 47 194 5.72e-20 89.4
Msa0689920 MtrunA17_Chr7g0268961 35.664 143 77 6 109 249 45 174 8.73e-20 89.0
Msa0689920 MtrunA17_Chr2g0326381 35.211 142 80 4 109 249 55 185 1.03e-19 88.6
Msa0689920 MtrunA17_Chr1g0191991 33.520 179 90 8 103 273 74 231 1.21e-19 89.0
Msa0689920 MtrunA17_Chr2g0330171 35.172 145 79 6 107 249 67 198 3.75e-19 87.0
Msa0689920 MtrunA17_Chr8g0373541 31.188 202 117 7 85 279 29 215 5.52e-19 86.7
Msa0689920 MtrunA17_Chr4g0040571 33.553 152 88 5 107 257 60 199 3.26e-18 84.3
Msa0689920 MtrunA17_Chr3g0141431 30.405 148 89 5 107 252 43 178 7.19e-17 80.1
Msa0689920 MtrunA17_Chr2g0324991 35.461 141 76 7 107 244 13 141 2.12e-16 74.7
Msa0689920 MtrunA17_Chr8g0375521 27.083 192 124 6 61 248 2 181 3.72e-13 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0689920 AT1G13290.1 65.447 246 61 5 23 258 12 243 5.62e-108 316
Msa0689920 AT3G57670.1 71.429 196 40 4 99 292 201 382 5.35e-98 293
Msa0689920 AT1G51220.1 75.152 165 38 1 100 264 163 324 5.89e-95 284
Msa0689920 AT3G20880.1 77.987 159 32 1 97 255 238 393 5.78e-94 284
Msa0689920 AT1G08290.1 73.810 168 38 3 99 265 164 326 4.23e-93 279
Msa0689920 AT1G34790.1 75.159 157 36 1 100 256 130 283 7.47e-88 265
Msa0689920 AT1G34370.3 41.176 153 84 5 107 255 27 177 3.13e-26 105
Msa0689920 AT1G34370.2 41.176 153 84 5 107 255 237 387 1.28e-25 106
Msa0689920 AT1G34370.1 41.176 153 84 5 107 255 237 387 1.28e-25 106
Msa0689920 AT5G22890.1 40.645 155 84 7 106 255 209 360 1.50e-24 102
Msa0689920 AT5G03150.1 35.211 142 80 4 109 249 76 206 5.34e-21 93.2
Msa0689920 AT1G55110.3 33.758 157 90 5 107 261 84 228 6.94e-21 92.4
Msa0689920 AT1G55110.1 33.758 157 90 5 107 261 84 228 6.94e-21 92.4
Msa0689920 AT1G55110.2 33.758 157 90 5 107 261 84 228 6.94e-21 92.4
Msa0689920 AT5G44160.1 32.178 202 106 8 63 257 20 197 2.26e-20 91.3
Msa0689920 AT2G02070.2 35.443 158 87 5 94 249 60 204 3.64e-20 90.9
Msa0689920 AT2G02070.1 35.443 158 87 5 94 249 60 204 3.64e-20 90.9
Msa0689920 AT2G02080.1 36.552 145 77 5 107 249 75 206 3.95e-20 90.5
Msa0689920 AT2G02080.4 36.552 145 77 5 107 249 75 206 3.95e-20 90.5
Msa0689920 AT2G02080.5 36.552 145 77 5 107 249 75 206 3.95e-20 90.5
Msa0689920 AT2G02080.3 36.552 145 77 5 107 249 75 206 3.95e-20 90.5
Msa0689920 AT3G45260.2 34.615 156 90 4 109 263 62 206 4.20e-20 90.1
Msa0689920 AT3G45260.1 34.615 156 90 4 109 263 62 206 4.20e-20 90.1
Msa0689920 AT5G60470.1 35.211 142 80 4 109 249 58 188 5.76e-20 89.7
Msa0689920 AT5G60470.3 35.211 142 80 4 109 249 63 193 7.00e-20 89.7
Msa0689920 AT5G60470.2 35.211 142 80 4 109 249 67 197 7.05e-20 89.7
Msa0689920 AT5G66730.1 34.000 150 84 5 107 254 53 189 1.07e-19 89.4
Msa0689920 AT1G14580.1 37.241 145 76 6 107 249 74 205 1.38e-19 89.0
Msa0689920 AT1G14580.2 37.241 145 76 6 107 249 74 205 1.38e-19 89.0
Msa0689920 AT1G14580.3 37.241 145 76 6 107 249 88 219 1.57e-19 88.6
Msa0689920 AT1G03840.2 31.638 177 107 6 83 257 35 199 2.82e-19 88.2
Msa0689920 AT2G02080.6 36.620 142 75 5 110 249 1 129 3.44e-19 87.4
Msa0689920 AT2G02080.2 36.620 142 75 5 110 249 1 129 3.44e-19 87.4
Msa0689920 AT3G50700.1 35.172 145 79 5 107 249 55 186 5.48e-19 87.0
Msa0689920 AT5G44160.2 34.667 150 85 5 109 257 21 158 1.07e-18 85.9
Msa0689920 AT1G03840.1 31.844 179 106 6 83 257 35 201 1.67e-18 85.9
Msa0689920 AT3G13810.4 32.051 156 94 4 107 261 91 235 2.39e-18 85.5
Msa0689920 AT3G13810.1 32.051 156 94 4 107 261 91 235 2.39e-18 85.5
Msa0689920 AT3G13810.5 32.051 156 94 4 107 261 86 230 2.44e-18 85.5
Msa0689920 AT3G13810.2 32.895 152 90 4 107 257 92 232 2.54e-18 85.1
Msa0689920 AT3G13810.6 32.051 156 94 4 107 261 72 216 2.65e-18 85.1
Msa0689920 AT3G13810.3 32.895 152 90 4 107 257 78 218 2.66e-18 85.1
Msa0689920 AT4G02670.1 34.028 144 83 4 107 249 74 206 4.20e-18 84.3
Msa0689920 AT4G02670.2 34.028 144 83 4 107 249 76 208 4.20e-18 84.3

Find 81 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCGAGTTTGGGAGGTATT+TGG 0.247423 4_4:-57249457 None:intergenic
CTCCCATTATTCCTCAATAT+TGG 0.256317 4_4:+57249630 Msa0689920:CDS
AGTGAAAGTTGGATTAATTA+AGG 0.285981 4_4:-57249591 None:intergenic
GATCCAATATTGAGGAATAA+TGG 0.295991 4_4:-57249633 None:intergenic
AAAGGACCGGAGTCTCTAAA+AGG 0.306782 4_4:+57250028 Msa0689920:CDS
GATGAAGATGATGCTGGTTT+TGG 0.319383 4_4:-57250052 None:intergenic
TGCAGACAGTGCAAGAAAAC+TGG 0.325433 4_4:-57249688 None:intergenic
TCTCCTGCTCAAATCCTTGT+TGG 0.331954 4_4:+57249659 Msa0689920:CDS
TTCCTCTCTCTTGCTCCTCC+TGG 0.347551 4_4:+57249431 Msa0689920:CDS
AAAGATCACGTTCGTGCGTT+TGG 0.349116 4_4:+57250328 Msa0689920:CDS
AGAAGAATTGTGGGAAGCTT+TGG 0.351421 4_4:+57250263 Msa0689920:CDS
AATGGGAGTGTGAGATGGTT+TGG 0.355414 4_4:-57249615 None:intergenic
AGGGATAGTAGTAGTAATAG+TGG 0.376112 4_4:-57249489 None:intergenic
AGAGAGGTAGTAGTAGAAAT+AGG 0.389319 4_4:-57249527 None:intergenic
ATCCAATATTGAGGAATAAT+GGG 0.396877 4_4:-57249632 None:intergenic
CACGTTCGTGCGTTTGGTAA+TGG 0.397696 4_4:+57250334 Msa0689920:CDS
CTACATTTACAGCACAAGTA+TGG 0.418258 4_4:-57249559 None:intergenic
GATGGTTTGGAAGTGAAAGT+TGG 0.427167 4_4:-57249602 None:intergenic
TCTTTCAGTGGTCTTGCCCT+TGG 0.430630 4_4:-57250133 None:intergenic
TAGTAGTAATAGTGGGATGA+GGG 0.441652 4_4:-57249481 None:intergenic
CATTTGCTGTGAGAGGAGAT+TGG 0.445706 4_4:+57250230 Msa0689920:CDS
TTGGGAGGTATTTGGTGGCC+AGG 0.460715 4_4:-57249449 None:intergenic
GGGGACATGGATCTCAATAT+AGG 0.462797 4_4:+57250005 Msa0689920:CDS
GTAGTAGTAATAGTGGGATG+AGG 0.468089 4_4:-57249482 None:intergenic
GAAATAGGTATTTGATGCAA+GGG 0.489738 4_4:-57249512 None:intergenic
CATGTTGTTGAAACGGTTGA+AGG 0.493614 4_4:-57249717 None:intergenic
TACATGGAAGAAGAAGAAAC+TGG 0.497808 4_4:+57250415 Msa0689920:CDS
AGGAGCAAGAGAGAGGAAGA+TGG 0.499455 4_4:-57249426 None:intergenic
GGGATAGTAGTAGTAATAGT+GGG 0.503268 4_4:-57249488 None:intergenic
CATTCGAGTGTAGGAAGTGT+AGG 0.505760 4_4:+57250203 Msa0689920:CDS
CGAGTTTGGGAGGTATTTGG+TGG 0.508108 4_4:-57249454 None:intergenic
CCATGTTACTGTTGTGCTGA+GGG 0.513723 4_4:+57250088 Msa0689920:CDS
AGAAATAGGTATTTGATGCA+AGG 0.514616 4_4:-57249513 None:intergenic
GGACACATGAGAAGAATTGT+GGG 0.524360 4_4:+57250254 Msa0689920:CDS
ACAAGGATTTGAGCAGGAGA+AGG 0.531708 4_4:-57249656 None:intergenic
CAACCGTTTCAACAACATGC+AGG 0.536317 4_4:+57249721 Msa0689920:CDS
ATTCCAACGTGTGGGGAGCA+TGG 0.539289 4_4:-57250357 None:intergenic
GAAACAACATAAACTATCCA+AGG 0.546681 4_4:+57250116 Msa0689920:CDS
GAACCTGCATGTTGTTGAAA+CGG 0.548476 4_4:-57249724 None:intergenic
AACTCAAAGATTCCAACGTG+TGG 0.551951 4_4:-57250366 None:intergenic
AGGACACATGAGAAGAATTG+TGG 0.552345 4_4:+57250253 Msa0689920:CDS
AGCATTGATGAAGATGATGC+TGG 0.557005 4_4:-57250058 None:intergenic
AAGGACCGGAGTCTCTAAAA+GGG 0.557565 4_4:+57250029 Msa0689920:CDS
ATCTAAGCTGCTGCTGCAAG+AGG 0.562751 4_4:-57249393 None:intergenic
TTGTATGGTAGATGCATATG+TGG 0.564484 4_4:+57249984 Msa0689920:intron
GGAGGTATTTGGTGGCCAGG+AGG 0.567766 4_4:-57249446 None:intergenic
GAGGGAAGTCATTCGAGTGT+AGG 0.568390 4_4:+57250194 Msa0689920:CDS
ATAGGTATTTGATGCAAGGG+AGG 0.571468 4_4:-57249509 None:intergenic
GGACCAACAAGGATTTGAGC+AGG 0.574385 4_4:-57249662 None:intergenic
ACTCAAAGATTCCAACGTGT+GGG 0.574474 4_4:-57250365 None:intergenic
ACACACTACAAAAGAAAGCA+TGG 0.577637 4_4:+57250172 Msa0689920:CDS
CAAGGATTTGAGCAGGAGAA+GGG 0.578543 4_4:-57249655 None:intergenic
TAAAGTAAATGAAAATTACA+TGG 0.583660 4_4:+57250399 Msa0689920:CDS
AGTAGTAATAGTGGGATGAG+GGG 0.585249 4_4:-57249480 None:intergenic
GCAGACAGTGCAAGAAAACT+GGG 0.587578 4_4:-57249687 None:intergenic
TTGCTGTGAGAGGAGATTGG+AGG 0.592461 4_4:+57250233 Msa0689920:CDS
GGCCAGGAGGAGCAAGAGAG+AGG 0.593784 4_4:-57249433 None:intergenic
GGAATAATGGGAGTGTGAGA+TGG 0.594723 4_4:-57249620 None:intergenic
ATCTCAATATAGGAAAGGAC+CGG 0.597133 4_4:+57250015 Msa0689920:CDS
AGGGTTCTGAAATCTTTCAG+TGG 0.606548 4_4:-57250145 None:intergenic
GGAGAAGGGATCCAATATTG+AGG 0.614687 4_4:-57249641 None:intergenic
ACCATGTTACTGTTGTGCTG+AGG 0.615026 4_4:+57250087 Msa0689920:CDS
CATGGATCTCAATATAGGAA+AGG 0.616598 4_4:+57250010 Msa0689920:CDS
TGTATGGTAGATGCATATGT+GGG 0.620648 4_4:+57249985 Msa0689920:intron
AGGAAGTCATTTGCTGTGAG+AGG 0.625916 4_4:+57250223 Msa0689920:CDS
CCCTCAGCACAACAGTAACA+TGG 0.627644 4_4:-57250088 None:intergenic
CTCAAAGATTCCAACGTGTG+GGG 0.627798 4_4:-57250364 None:intergenic
GTAGTAATAGTGGGATGAGG+GGG 0.632310 4_4:-57249479 None:intergenic
TAGATGCATATGTGGGGACA+TGG 0.632523 4_4:+57249992 Msa0689920:intron
TCTAAGCTGCTGCTGCAAGA+GGG 0.633302 4_4:-57249392 None:intergenic
AGTATGGTCATGGTGAAGAG+AGG 0.640967 4_4:-57249543 None:intergenic
TAGGTATTTGATGCAAGGGA+GGG 0.641902 4_4:-57249508 None:intergenic
ACTACAAAAGAAAGCATGGA+GGG 0.646182 4_4:+57250176 Msa0689920:CDS
TGGCCATGCTCCCCACACGT+TGG 0.648703 4_4:+57250354 Msa0689920:CDS
AAACAACATAAACTATCCAA+GGG 0.649476 4_4:+57250117 Msa0689920:CDS
TTACAGCACAAGTATGGTCA+TGG 0.652632 4_4:-57249553 None:intergenic
ACAGTGCAAGAAAACTGGGT+AGG 0.667615 4_4:-57249683 None:intergenic
AAAACTGGGTAGGACCAACA+AGG 0.677453 4_4:-57249673 None:intergenic
GTACTGATGATGAGTTTGCA+TGG 0.690956 4_4:-57249348 None:intergenic
CACTACAAAAGAAAGCATGG+AGG 0.735997 4_4:+57250175 Msa0689920:CDS
GTATGGTAGATGCATATGTG+GGG 0.763722 4_4:+57249986 Msa0689920:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGGATAAAAATATAATAA+TGG - chr4_4:57249752-57249771 None:intergenic 10.0%
!! AAAGGATAAAAATATAATAA+TGG - chr4_4:57249752-57249771 None:intergenic 10.0%
!! TCATGATATAGTAAAAATAA+AGG - chr4_4:57249770-57249789 None:intergenic 15.0%
!! CTAATGAATGATTAATTTAA+AGG - chr4_4:57249841-57249860 None:intergenic 15.0%
!! AAACATGATTCATATATATA+TGG + chr4_4:57249939-57249958 Msa0689920:intron 15.0%
!! TAAAGTAAATGAAAATTACA+TGG + chr4_4:57250399-57250418 Msa0689920:CDS 15.0%
!! TCATGATATAGTAAAAATAA+AGG - chr4_4:57249770-57249789 None:intergenic 15.0%
!! CTAATGAATGATTAATTTAA+AGG - chr4_4:57249841-57249860 None:intergenic 15.0%
!! AAACATGATTCATATATATA+TGG + chr4_4:57249939-57249958 Msa0689920:intron 15.0%
!! TAAAGTAAATGAAAATTACA+TGG + chr4_4:57250399-57250418 Msa0689920:CDS 15.0%
!! TATGATATTGTTGTATTGTA+TGG + chr4_4:57249969-57249988 Msa0689920:intron 20.0%
!! TATGATATTGTTGTATTGTA+TGG + chr4_4:57249969-57249988 Msa0689920:intron 20.0%
! AGTGAAAGTTGGATTAATTA+AGG - chr4_4:57249594-57249613 None:intergenic 25.0%
! ATCCAATATTGAGGAATAAT+GGG - chr4_4:57249635-57249654 None:intergenic 25.0%
! GAATGATTAATTTAAAGGCA+AGG - chr4_4:57249836-57249855 None:intergenic 25.0%
! AAACAACATAAACTATCCAA+GGG + chr4_4:57250117-57250136 Msa0689920:CDS 25.0%
! AGTGAAAGTTGGATTAATTA+AGG - chr4_4:57249594-57249613 None:intergenic 25.0%
! ATCCAATATTGAGGAATAAT+GGG - chr4_4:57249635-57249654 None:intergenic 25.0%
! GAATGATTAATTTAAAGGCA+AGG - chr4_4:57249836-57249855 None:intergenic 25.0%
! AAACAACATAAACTATCCAA+GGG + chr4_4:57250117-57250136 Msa0689920:CDS 25.0%
GAAATAGGTATTTGATGCAA+GGG - chr4_4:57249515-57249534 None:intergenic 30.0%
AGAAATAGGTATTTGATGCA+AGG - chr4_4:57249516-57249535 None:intergenic 30.0%
GATCCAATATTGAGGAATAA+TGG - chr4_4:57249636-57249655 None:intergenic 30.0%
TCATTCATGGAAGAAAAGTA+AGG - chr4_4:57249890-57249909 None:intergenic 30.0%
AGCTCAATATGATTCATTCA+TGG - chr4_4:57249903-57249922 None:intergenic 30.0%
GAAACAACATAAACTATCCA+AGG + chr4_4:57250116-57250135 Msa0689920:CDS 30.0%
! TCTTTTGTAGTGTGTTTGAA+GGG - chr4_4:57250167-57250186 None:intergenic 30.0%
! TTCTTTTGTAGTGTGTTTGA+AGG - chr4_4:57250168-57250187 None:intergenic 30.0%
!! TTGGAATCTTTGAGTTTTGA+TGG + chr4_4:57250373-57250392 Msa0689920:CDS 30.0%
GAAATAGGTATTTGATGCAA+GGG - chr4_4:57249515-57249534 None:intergenic 30.0%
AGAAATAGGTATTTGATGCA+AGG - chr4_4:57249516-57249535 None:intergenic 30.0%
GATCCAATATTGAGGAATAA+TGG - chr4_4:57249636-57249655 None:intergenic 30.0%
TCATTCATGGAAGAAAAGTA+AGG - chr4_4:57249890-57249909 None:intergenic 30.0%
AGCTCAATATGATTCATTCA+TGG - chr4_4:57249903-57249922 None:intergenic 30.0%
GAAACAACATAAACTATCCA+AGG + chr4_4:57250116-57250135 Msa0689920:CDS 30.0%
! TCTTTTGTAGTGTGTTTGAA+GGG - chr4_4:57250167-57250186 None:intergenic 30.0%
! TTCTTTTGTAGTGTGTTTGA+AGG - chr4_4:57250168-57250187 None:intergenic 30.0%
!! TTGGAATCTTTGAGTTTTGA+TGG + chr4_4:57250373-57250392 Msa0689920:CDS 30.0%
! TAGTAGTAATAGTGGGATGA+GGG - chr4_4:57249484-57249503 None:intergenic 35.0%
GGGATAGTAGTAGTAATAGT+GGG - chr4_4:57249491-57249510 None:intergenic 35.0%
AGGGATAGTAGTAGTAATAG+TGG - chr4_4:57249492-57249511 None:intergenic 35.0%
AGAGAGGTAGTAGTAGAAAT+AGG - chr4_4:57249530-57249549 None:intergenic 35.0%
CTACATTTACAGCACAAGTA+TGG - chr4_4:57249562-57249581 None:intergenic 35.0%
CTCCCATTATTCCTCAATAT+TGG + chr4_4:57249630-57249649 Msa0689920:CDS 35.0%
TTGTATGGTAGATGCATATG+TGG + chr4_4:57249984-57250003 Msa0689920:intron 35.0%
TGTATGGTAGATGCATATGT+GGG + chr4_4:57249985-57250004 Msa0689920:intron 35.0%
CATGGATCTCAATATAGGAA+AGG + chr4_4:57250010-57250029 Msa0689920:CDS 35.0%
ATCTCAATATAGGAAAGGAC+CGG + chr4_4:57250015-57250034 Msa0689920:CDS 35.0%
ACACACTACAAAAGAAAGCA+TGG + chr4_4:57250172-57250191 Msa0689920:CDS 35.0%
! ACTACAAAAGAAAGCATGGA+GGG + chr4_4:57250176-57250195 Msa0689920:CDS 35.0%
!!! AAGCTTTGGTTTTGTATCTG+TGG + chr4_4:57250277-57250296 Msa0689920:CDS 35.0%
TACATGGAAGAAGAAGAAAC+TGG + chr4_4:57250415-57250434 Msa0689920:CDS 35.0%
! TAGTAGTAATAGTGGGATGA+GGG - chr4_4:57249484-57249503 None:intergenic 35.0%
GGGATAGTAGTAGTAATAGT+GGG - chr4_4:57249491-57249510 None:intergenic 35.0%
AGGGATAGTAGTAGTAATAG+TGG - chr4_4:57249492-57249511 None:intergenic 35.0%
AGAGAGGTAGTAGTAGAAAT+AGG - chr4_4:57249530-57249549 None:intergenic 35.0%
CTACATTTACAGCACAAGTA+TGG - chr4_4:57249562-57249581 None:intergenic 35.0%
CTCCCATTATTCCTCAATAT+TGG + chr4_4:57249630-57249649 Msa0689920:CDS 35.0%
TTGTATGGTAGATGCATATG+TGG + chr4_4:57249984-57250003 Msa0689920:intron 35.0%
TGTATGGTAGATGCATATGT+GGG + chr4_4:57249985-57250004 Msa0689920:intron 35.0%
CATGGATCTCAATATAGGAA+AGG + chr4_4:57250010-57250029 Msa0689920:CDS 35.0%
ATCTCAATATAGGAAAGGAC+CGG + chr4_4:57250015-57250034 Msa0689920:CDS 35.0%
ACACACTACAAAAGAAAGCA+TGG + chr4_4:57250172-57250191 Msa0689920:CDS 35.0%
! ACTACAAAAGAAAGCATGGA+GGG + chr4_4:57250176-57250195 Msa0689920:CDS 35.0%
!!! AAGCTTTGGTTTTGTATCTG+TGG + chr4_4:57250277-57250296 Msa0689920:CDS 35.0%
TACATGGAAGAAGAAGAAAC+TGG + chr4_4:57250415-57250434 Msa0689920:CDS 35.0%
! AGTAGTAATAGTGGGATGAG+GGG - chr4_4:57249483-57249502 None:intergenic 40.0%
! GTAGTAGTAATAGTGGGATG+AGG - chr4_4:57249485-57249504 None:intergenic 40.0%
TAGGTATTTGATGCAAGGGA+GGG - chr4_4:57249511-57249530 None:intergenic 40.0%
ATAGGTATTTGATGCAAGGG+AGG - chr4_4:57249512-57249531 None:intergenic 40.0%
TTACAGCACAAGTATGGTCA+TGG - chr4_4:57249556-57249575 None:intergenic 40.0%
! GATGGTTTGGAAGTGAAAGT+TGG - chr4_4:57249605-57249624 None:intergenic 40.0%
CATGTTGTTGAAACGGTTGA+AGG - chr4_4:57249720-57249739 None:intergenic 40.0%
GAACCTGCATGTTGTTGAAA+CGG - chr4_4:57249727-57249746 None:intergenic 40.0%
GTATGGTAGATGCATATGTG+GGG + chr4_4:57249986-57250005 Msa0689920:intron 40.0%
!!! GATGAAGATGATGCTGGTTT+TGG - chr4_4:57250055-57250074 None:intergenic 40.0%
!! AGCATTGATGAAGATGATGC+TGG - chr4_4:57250061-57250080 None:intergenic 40.0%
!! AGGGTTCTGAAATCTTTCAG+TGG - chr4_4:57250148-57250167 None:intergenic 40.0%
! CACTACAAAAGAAAGCATGG+AGG + chr4_4:57250175-57250194 Msa0689920:CDS 40.0%
AGGACACATGAGAAGAATTG+TGG + chr4_4:57250253-57250272 Msa0689920:CDS 40.0%
GGACACATGAGAAGAATTGT+GGG + chr4_4:57250254-57250273 Msa0689920:CDS 40.0%
AGAAGAATTGTGGGAAGCTT+TGG + chr4_4:57250263-57250282 Msa0689920:CDS 40.0%
ACTCAAAGATTCCAACGTGT+GGG - chr4_4:57250368-57250387 None:intergenic 40.0%
AACTCAAAGATTCCAACGTG+TGG - chr4_4:57250369-57250388 None:intergenic 40.0%
! AGTAGTAATAGTGGGATGAG+GGG - chr4_4:57249483-57249502 None:intergenic 40.0%
! GTAGTAGTAATAGTGGGATG+AGG - chr4_4:57249485-57249504 None:intergenic 40.0%
TAGGTATTTGATGCAAGGGA+GGG - chr4_4:57249511-57249530 None:intergenic 40.0%
ATAGGTATTTGATGCAAGGG+AGG - chr4_4:57249512-57249531 None:intergenic 40.0%
TTACAGCACAAGTATGGTCA+TGG - chr4_4:57249556-57249575 None:intergenic 40.0%
! GATGGTTTGGAAGTGAAAGT+TGG - chr4_4:57249605-57249624 None:intergenic 40.0%
CATGTTGTTGAAACGGTTGA+AGG - chr4_4:57249720-57249739 None:intergenic 40.0%
GAACCTGCATGTTGTTGAAA+CGG - chr4_4:57249727-57249746 None:intergenic 40.0%
GTATGGTAGATGCATATGTG+GGG + chr4_4:57249986-57250005 Msa0689920:intron 40.0%
!!! GATGAAGATGATGCTGGTTT+TGG - chr4_4:57250055-57250074 None:intergenic 40.0%
!! AGCATTGATGAAGATGATGC+TGG - chr4_4:57250061-57250080 None:intergenic 40.0%
!! AGGGTTCTGAAATCTTTCAG+TGG - chr4_4:57250148-57250167 None:intergenic 40.0%
! CACTACAAAAGAAAGCATGG+AGG + chr4_4:57250175-57250194 Msa0689920:CDS 40.0%
AGGACACATGAGAAGAATTG+TGG + chr4_4:57250253-57250272 Msa0689920:CDS 40.0%
GGACACATGAGAAGAATTGT+GGG + chr4_4:57250254-57250273 Msa0689920:CDS 40.0%
AGAAGAATTGTGGGAAGCTT+TGG + chr4_4:57250263-57250282 Msa0689920:CDS 40.0%
ACTCAAAGATTCCAACGTGT+GGG - chr4_4:57250368-57250387 None:intergenic 40.0%
AACTCAAAGATTCCAACGTG+TGG - chr4_4:57250369-57250388 None:intergenic 40.0%
! GTAGTAATAGTGGGATGAGG+GGG - chr4_4:57249482-57249501 None:intergenic 45.0%
AGTATGGTCATGGTGAAGAG+AGG - chr4_4:57249546-57249565 None:intergenic 45.0%
! AATGGGAGTGTGAGATGGTT+TGG - chr4_4:57249618-57249637 None:intergenic 45.0%
! GGAATAATGGGAGTGTGAGA+TGG - chr4_4:57249623-57249642 None:intergenic 45.0%
GGAGAAGGGATCCAATATTG+AGG - chr4_4:57249644-57249663 None:intergenic 45.0%
CAAGGATTTGAGCAGGAGAA+GGG - chr4_4:57249658-57249677 None:intergenic 45.0%
ACAAGGATTTGAGCAGGAGA+AGG - chr4_4:57249659-57249678 None:intergenic 45.0%
TCTCCTGCTCAAATCCTTGT+TGG + chr4_4:57249659-57249678 Msa0689920:CDS 45.0%
AAAACTGGGTAGGACCAACA+AGG - chr4_4:57249676-57249695 None:intergenic 45.0%
ACAGTGCAAGAAAACTGGGT+AGG - chr4_4:57249686-57249705 None:intergenic 45.0%
GCAGACAGTGCAAGAAAACT+GGG - chr4_4:57249690-57249709 None:intergenic 45.0%
TGCAGACAGTGCAAGAAAAC+TGG - chr4_4:57249691-57249710 None:intergenic 45.0%
CAACCGTTTCAACAACATGC+AGG + chr4_4:57249721-57249740 Msa0689920:CDS 45.0%
TAGATGCATATGTGGGGACA+TGG + chr4_4:57249992-57250011 Msa0689920:intron 45.0%
GGGGACATGGATCTCAATAT+AGG + chr4_4:57250005-57250024 Msa0689920:CDS 45.0%
AAAGGACCGGAGTCTCTAAA+AGG + chr4_4:57250028-57250047 Msa0689920:CDS 45.0%
! AAGGACCGGAGTCTCTAAAA+GGG + chr4_4:57250029-57250048 Msa0689920:CDS 45.0%
!! TTTGGCCCTTTTAGAGACTC+CGG - chr4_4:57250037-57250056 None:intergenic 45.0%
ACCATGTTACTGTTGTGCTG+AGG + chr4_4:57250087-57250106 Msa0689920:CDS 45.0%
CCATGTTACTGTTGTGCTGA+GGG + chr4_4:57250088-57250107 Msa0689920:CDS 45.0%
! CATTCGAGTGTAGGAAGTGT+AGG + chr4_4:57250203-57250222 Msa0689920:CDS 45.0%
!! AGGAAGTCATTTGCTGTGAG+AGG + chr4_4:57250223-57250242 Msa0689920:CDS 45.0%
!! CATTTGCTGTGAGAGGAGAT+TGG + chr4_4:57250230-57250249 Msa0689920:CDS 45.0%
AAAGATCACGTTCGTGCGTT+TGG + chr4_4:57250328-57250347 Msa0689920:CDS 45.0%
CTCAAAGATTCCAACGTGTG+GGG - chr4_4:57250367-57250386 None:intergenic 45.0%
! GTAGTAATAGTGGGATGAGG+GGG - chr4_4:57249482-57249501 None:intergenic 45.0%
AGTATGGTCATGGTGAAGAG+AGG - chr4_4:57249546-57249565 None:intergenic 45.0%
! AATGGGAGTGTGAGATGGTT+TGG - chr4_4:57249618-57249637 None:intergenic 45.0%
! GGAATAATGGGAGTGTGAGA+TGG - chr4_4:57249623-57249642 None:intergenic 45.0%
GGAGAAGGGATCCAATATTG+AGG - chr4_4:57249644-57249663 None:intergenic 45.0%
CAAGGATTTGAGCAGGAGAA+GGG - chr4_4:57249658-57249677 None:intergenic 45.0%
ACAAGGATTTGAGCAGGAGA+AGG - chr4_4:57249659-57249678 None:intergenic 45.0%
TCTCCTGCTCAAATCCTTGT+TGG + chr4_4:57249659-57249678 Msa0689920:CDS 45.0%
AAAACTGGGTAGGACCAACA+AGG - chr4_4:57249676-57249695 None:intergenic 45.0%
ACAGTGCAAGAAAACTGGGT+AGG - chr4_4:57249686-57249705 None:intergenic 45.0%
GCAGACAGTGCAAGAAAACT+GGG - chr4_4:57249690-57249709 None:intergenic 45.0%
TGCAGACAGTGCAAGAAAAC+TGG - chr4_4:57249691-57249710 None:intergenic 45.0%
CAACCGTTTCAACAACATGC+AGG + chr4_4:57249721-57249740 Msa0689920:CDS 45.0%
TAGATGCATATGTGGGGACA+TGG + chr4_4:57249992-57250011 Msa0689920:intron 45.0%
GGGGACATGGATCTCAATAT+AGG + chr4_4:57250005-57250024 Msa0689920:CDS 45.0%
AAAGGACCGGAGTCTCTAAA+AGG + chr4_4:57250028-57250047 Msa0689920:CDS 45.0%
! AAGGACCGGAGTCTCTAAAA+GGG + chr4_4:57250029-57250048 Msa0689920:CDS 45.0%
!! TTTGGCCCTTTTAGAGACTC+CGG - chr4_4:57250037-57250056 None:intergenic 45.0%
ACCATGTTACTGTTGTGCTG+AGG + chr4_4:57250087-57250106 Msa0689920:CDS 45.0%
CCATGTTACTGTTGTGCTGA+GGG + chr4_4:57250088-57250107 Msa0689920:CDS 45.0%
! CATTCGAGTGTAGGAAGTGT+AGG + chr4_4:57250203-57250222 Msa0689920:CDS 45.0%
!! AGGAAGTCATTTGCTGTGAG+AGG + chr4_4:57250223-57250242 Msa0689920:CDS 45.0%
!! CATTTGCTGTGAGAGGAGAT+TGG + chr4_4:57250230-57250249 Msa0689920:CDS 45.0%
AAAGATCACGTTCGTGCGTT+TGG + chr4_4:57250328-57250347 Msa0689920:CDS 45.0%
CTCAAAGATTCCAACGTGTG+GGG - chr4_4:57250367-57250386 None:intergenic 45.0%
TCTAAGCTGCTGCTGCAAGA+GGG - chr4_4:57249395-57249414 None:intergenic 50.0%
ATCTAAGCTGCTGCTGCAAG+AGG - chr4_4:57249396-57249415 None:intergenic 50.0%
AGGAGCAAGAGAGAGGAAGA+TGG - chr4_4:57249429-57249448 None:intergenic 50.0%
!! CGAGTTTGGGAGGTATTTGG+TGG - chr4_4:57249457-57249476 None:intergenic 50.0%
!! GTGCGAGTTTGGGAGGTATT+TGG - chr4_4:57249460-57249479 None:intergenic 50.0%
GGACCAACAAGGATTTGAGC+AGG - chr4_4:57249665-57249684 None:intergenic 50.0%
CCCTCAGCACAACAGTAACA+TGG - chr4_4:57250091-57250110 None:intergenic 50.0%
! TCTTTCAGTGGTCTTGCCCT+TGG - chr4_4:57250136-57250155 None:intergenic 50.0%
GAGGGAAGTCATTCGAGTGT+AGG + chr4_4:57250194-57250213 Msa0689920:CDS 50.0%
TTGCTGTGAGAGGAGATTGG+AGG + chr4_4:57250233-57250252 Msa0689920:CDS 50.0%
CACGTTCGTGCGTTTGGTAA+TGG + chr4_4:57250334-57250353 Msa0689920:CDS 50.0%
TCTAAGCTGCTGCTGCAAGA+GGG - chr4_4:57249395-57249414 None:intergenic 50.0%
ATCTAAGCTGCTGCTGCAAG+AGG - chr4_4:57249396-57249415 None:intergenic 50.0%
AGGAGCAAGAGAGAGGAAGA+TGG - chr4_4:57249429-57249448 None:intergenic 50.0%
!! CGAGTTTGGGAGGTATTTGG+TGG - chr4_4:57249457-57249476 None:intergenic 50.0%
!! GTGCGAGTTTGGGAGGTATT+TGG - chr4_4:57249460-57249479 None:intergenic 50.0%
GGACCAACAAGGATTTGAGC+AGG - chr4_4:57249665-57249684 None:intergenic 50.0%
CCCTCAGCACAACAGTAACA+TGG - chr4_4:57250091-57250110 None:intergenic 50.0%
! TCTTTCAGTGGTCTTGCCCT+TGG - chr4_4:57250136-57250155 None:intergenic 50.0%
GAGGGAAGTCATTCGAGTGT+AGG + chr4_4:57250194-57250213 Msa0689920:CDS 50.0%
TTGCTGTGAGAGGAGATTGG+AGG + chr4_4:57250233-57250252 Msa0689920:CDS 50.0%
CACGTTCGTGCGTTTGGTAA+TGG + chr4_4:57250334-57250353 Msa0689920:CDS 50.0%
TTCCTCTCTCTTGCTCCTCC+TGG + chr4_4:57249431-57249450 Msa0689920:CDS 55.0%
!! TTGGGAGGTATTTGGTGGCC+AGG - chr4_4:57249452-57249471 None:intergenic 55.0%
ATTCCAACGTGTGGGGAGCA+TGG - chr4_4:57250360-57250379 None:intergenic 55.0%
TTCCTCTCTCTTGCTCCTCC+TGG + chr4_4:57249431-57249450 Msa0689920:CDS 55.0%
!! TTGGGAGGTATTTGGTGGCC+AGG - chr4_4:57249452-57249471 None:intergenic 55.0%
ATTCCAACGTGTGGGGAGCA+TGG - chr4_4:57250360-57250379 None:intergenic 55.0%
!! GGAGGTATTTGGTGGCCAGG+AGG - chr4_4:57249449-57249468 None:intergenic 60.0%
! GGATGAGGGGGTGCGAGTTT+GGG - chr4_4:57249470-57249489 None:intergenic 60.0%
!! GGAGGTATTTGGTGGCCAGG+AGG - chr4_4:57249449-57249468 None:intergenic 60.0%
! GGATGAGGGGGTGCGAGTTT+GGG - chr4_4:57249470-57249489 None:intergenic 60.0%
GGCCAGGAGGAGCAAGAGAG+AGG - chr4_4:57249436-57249455 None:intergenic 65.0%
! TGAGGGGGTGCGAGTTTGGG+AGG - chr4_4:57249467-57249486 None:intergenic 65.0%
! GGGATGAGGGGGTGCGAGTT+TGG - chr4_4:57249471-57249490 None:intergenic 65.0%
TGGCCATGCTCCCCACACGT+TGG + chr4_4:57250354-57250373 Msa0689920:CDS 65.0%
GGCCAGGAGGAGCAAGAGAG+AGG - chr4_4:57249436-57249455 None:intergenic 65.0%
! TGAGGGGGTGCGAGTTTGGG+AGG - chr4_4:57249467-57249486 None:intergenic 65.0%
! GGGATGAGGGGGTGCGAGTT+TGG - chr4_4:57249471-57249490 None:intergenic 65.0%
TGGCCATGCTCCCCACACGT+TGG + chr4_4:57250354-57250373 Msa0689920:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 57249350 57250486 57249350 ID=Msa0689920;Name=Msa0689920
chr4_4 mRNA 57249350 57250486 57249350 ID=Msa0689920-mRNA-1;Parent=Msa0689920;Name=Msa0689920-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|294
chr4_4 exon 57249350 57249742 57249350 ID=Msa0689920-mRNA-1:exon:15251;Parent=Msa0689920-mRNA-1
chr4_4 exon 57249995 57250486 57249995 ID=Msa0689920-mRNA-1:exon:15252;Parent=Msa0689920-mRNA-1
chr4_4 CDS 57249350 57249742 57249350 ID=Msa0689920-mRNA-1:cds;Parent=Msa0689920-mRNA-1
chr4_4 CDS 57249995 57250486 57249995 ID=Msa0689920-mRNA-1:cds;Parent=Msa0689920-mRNA-1
Gene Sequence

>Msa0689920

ATGCAAACTCATCATCAGTACTACTCATTCTCTTGTGAGAGTCCCTCTTGCAGCAGCAGCTTAGATGATGAAGATTCCATCTTCCTCTCTCTTGCTCCTCCTGGCCACCAAATACCTCCCAAACTCGCACCCCCTCATCCCACTATTACTACTACTATCCCTCCCTTGCATCAAATACCTATTTCTACTACTACCTCTCTTCACCATGACCATACTTGTGCTGTAAATGTAGCACTTAATGCCTTAATTAATCCAACTTTCACTTCCAAACCATCTCACACTCCCATTATTCCTCAATATTGGATCCCTTCTCCTGCTCAAATCCTTGTTGGTCCTACCCAGTTTTCTTGCACTGTCTGCAACAAAACCTTCAACCGTTTCAACAACATGCAGATGCATATGTGGGGACATGGATCTCAATATAGGAAAGGACCGGAGTCTCTAAAAGGGCCAAAACCAGCATCATCTTCATCAATGCTGAAACTACCATGTTACTGTTGTGCTGAGGGTTGCAGAAACAACATAAACTATCCAAGGGCAAGACCACTGAAAGATTTCAGAACCCTTCAAACACACTACAAAAGAAAGCATGGAGGGAAGTCATTCGAGTGTAGGAAGTGTAGGAAGTCATTTGCTGTGAGAGGAGATTGGAGGACACATGAGAAGAATTGTGGGAAGCTTTGGTTTTGTATCTGTGGTTCTGATTTCAAACACAAAAGATCACTAAAAGATCACGTTCGTGCGTTTGGTAATGGCCATGCTCCCCACACGTTGGAATCTTTGAGTTTTGATGGACATAAAGTAAATGAAAATTACATGGAAGAAGAAGAAACTGGAAGTGAAGATGAAGACAATAACAGTGAAGTTGTTTTTAGTTTGAATTAA
Protein sequence

>Msa0689920

MQTHHQYYSFSCESPSCSSSLDDEDSIFLSLAPPGHQIPPKLAPPHPTITTTIPPLHQIPISTTTSLHHDHTCAVNVALNALINPTFTSKPSHTPIIPQYWIPSPAQILVGPTQFSCTVCNKTFNRFNNMQMHMWGHGSQYRKGPESLKGPKPASSSSMLKLPCYCCAEGCRNNINYPRARPLKDFRTLQTHYKRKHGGKSFECRKCRKSFAVRGDWRTHEKNCGKLWFCICGSDFKHKRSLKDHVRAFGNGHAPHTLESLSFDGHKVNENYMEEEETGSEDEDNNSEVVFSLN*