AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0693510


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0693510 MtrunA17_Chr4g0043891 92.800 125 2 2 11 135 1 118 2.50e-78 232
Msa0693510 MtrunA17_Chr8g0392991 60.345 116 35 3 16 131 94 198 3.41e-37 130
Msa0693510 MtrunA17_Chr7g0238431 71.429 70 20 0 11 80 1 70 1.16e-31 112
Msa0693510 MtrunA17_Chr5g0436661 39.552 134 71 2 18 141 33 166 3.40e-28 102
Msa0693510 MtrunA17_Chr5g0405351 67.164 67 21 1 14 79 26 92 2.13e-27 103
Msa0693510 MtrunA17_Chr1g0153171 68.750 64 20 0 17 80 89 152 1.07e-26 100
Msa0693510 MtrunA17_Chr3g0125111 84.615 52 8 0 29 80 91 142 1.70e-26 97.4
Msa0693510 MtrunA17_Chr8g0385441 84.615 52 8 0 29 80 93 144 1.74e-26 97.4
Msa0693510 MtrunA17_Chr4g0031341 66.667 63 21 0 18 80 25 87 5.33e-26 98.6
Msa0693510 MtrunA17_Chr7g0223471 46.377 69 37 0 13 81 42 110 1.11e-18 80.9
Msa0693510 MtrunA17_Chr1g0212511 46.341 82 38 1 18 99 85 160 4.80e-18 77.8
Msa0693510 MtrunA17_Chr2g0283141 44.928 69 38 0 13 81 50 118 6.29e-18 79.0
Msa0693510 MtrunA17_Chr3g0144021 42.623 61 35 0 18 78 82 142 1.01e-13 65.5
Msa0693510 MtrunA17_Chr6g0460691 39.474 76 46 0 17 92 31 106 2.57e-13 65.5
Msa0693510 MtrunA17_Chr7g0222981 36.559 93 49 1 18 110 68 150 6.68e-13 62.8
Msa0693510 MtrunA17_Chr7g0254251 45.098 51 28 0 17 67 27 77 4.77e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0693510 AT3G18010.1 54.472 123 44 3 16 135 74 187 4.04e-36 127
Msa0693510 AT2G28610.1 65.714 70 24 0 11 80 1 70 5.52e-30 108
Msa0693510 AT5G59340.1 64.706 68 24 0 16 83 12 79 1.44e-27 103
Msa0693510 AT3G11260.1 48.421 95 39 1 18 102 24 118 9.56e-27 99.0
Msa0693510 AT2G17950.1 60.759 79 30 1 14 91 34 112 2.20e-26 100
Msa0693510 AT1G46480.2 63.768 69 24 1 12 80 85 152 3.89e-26 97.8
Msa0693510 AT2G01500.1 74.545 55 14 0 18 72 61 115 8.62e-26 98.6
Msa0693510 AT1G46480.1 63.768 69 24 1 12 80 85 152 2.02e-25 97.4
Msa0693510 AT5G05770.1 59.375 64 26 0 18 81 29 92 1.64e-23 89.0
Msa0693510 AT2G33880.1 44.286 70 39 0 13 82 50 119 1.69e-17 77.8
Msa0693510 AT2G33880.2 44.286 70 39 0 13 82 50 119 2.42e-17 77.4
Msa0693510 AT5G45980.1 44.776 67 37 0 13 79 50 116 4.15e-16 73.6
Msa0693510 AT4G35550.1 49.180 61 31 0 18 78 99 159 1.16e-15 72.0
Msa0693510 AT1G20700.2 45.000 60 33 0 18 77 33 92 3.74e-14 65.5
Msa0693510 AT1G20700.3 45.000 60 33 0 18 77 95 154 1.31e-13 65.5
Msa0693510 AT1G20700.1 45.000 60 33 0 18 77 95 154 1.45e-13 65.5
Msa0693510 AT3G03660.1 49.057 53 27 0 17 69 32 84 3.20e-13 65.1
Msa0693510 AT3G03660.2 49.057 53 27 0 17 69 32 84 5.09e-13 64.7
Msa0693510 AT3G03660.4 49.057 53 27 0 17 69 32 84 7.81e-13 64.3
Msa0693510 AT3G03660.3 49.057 53 27 0 17 69 32 84 1.02e-12 64.3
Msa0693510 AT5G17810.1 43.396 53 30 0 17 69 20 72 2.19e-11 60.1
Msa0693510 AT5G17810.2 43.396 53 30 0 17 69 20 72 2.19e-11 60.1
Msa0693510 AT5G17810.3 43.396 53 30 0 17 69 20 72 2.22e-11 60.1

Find 29 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTCTCTCGCCTTGTGATTC+TGG 0.194023 4_4:-62451323 None:intergenic
GCTTCGCAGCTTCGACAATT+TGG 0.206061 4_4:+62451267 Msa0693510:CDS
ATTTCTCTTGTCTTTGTTGA+TGG 0.263889 4_4:+62452015 Msa0693510:CDS
AGAGAAAGAGAAGTACGTTA+TGG 0.359866 4_4:+62451410 Msa0693510:CDS
CGACAATTTGGAAAGATTGA+AGG 0.396848 4_4:+62451279 Msa0693510:CDS
GAGAAAGAGAAGTACGTTAT+GGG 0.401887 4_4:+62451411 Msa0693510:CDS
GAAGCTGCGAAGCTATTTGT+TGG 0.428703 4_4:-62451257 None:intergenic
CTTGCCTATACAATTCTTCA+AGG 0.428836 4_4:-62451206 None:intergenic
TAGTCCTTGAAGAATTGTAT+AGG 0.431151 4_4:+62451202 Msa0693510:CDS
GTTGGACTCCACCTTGAACT+TGG 0.444756 4_4:-62451165 None:intergenic
CTTGAGATGGAGGAAACAAT+TGG 0.464032 4_4:+62451363 Msa0693510:CDS
TGCATCCTCAACCAAGTTCA+AGG 0.472729 4_4:+62451154 Msa0693510:CDS
CTCCACCTTGAACTTGGTTG+AGG 0.502185 4_4:-62451159 None:intergenic
TGTTGGATTTGTTCAGCTGA+TGG 0.503667 4_4:-62451240 None:intergenic
TTGTTCACTGCAGTTTGAAG+TGG 0.506780 4_4:-62451979 None:intergenic
TCAAGGACTAGTAATTGAAC+TGG 0.509163 4_4:-62451189 None:intergenic
GAAGCGTCGTCGCCTTGAGA+TGG 0.530642 4_4:+62451350 Msa0693510:CDS
ACTAGTAATTGAACTGGTGT+TGG 0.537149 4_4:-62451183 None:intergenic
CTTGAAGAATTGTATAGGCA+AGG 0.540070 4_4:+62451207 Msa0693510:CDS
TCAGAAAAGCAAGAAGCTGC+AGG 0.540109 4_4:+62451438 Msa0693510:CDS
CAATTGTTTCCTCCATCTCA+AGG 0.548253 4_4:-62451362 None:intergenic
GCGTCGTCGCCTTGAGATGG+AGG 0.576407 4_4:+62451353 Msa0693510:CDS
TTGAAGAATTGTATAGGCAA+GGG 0.590766 4_4:+62451208 Msa0693510:CDS
ACAGCTCATGCAGCTGAGCA+AGG 0.595931 4_4:+62451117 None:intergenic
AAACTGCAGTGAACAAGCAG+AGG 0.632631 4_4:+62451986 Msa0693510:CDS
AGTTAAAGAGACAAAGAAGT+GGG 0.667079 4_4:+62451953 Msa0693510:CDS
CTATTGGTTCCAGAATCACA+AGG 0.685006 4_4:+62451314 Msa0693510:CDS
GAGTTAAAGAGACAAAGAAG+TGG 0.689037 4_4:+62451952 Msa0693510:CDS
ATCCTCAACCAAGTTCAAGG+TGG 0.762805 4_4:+62451157 Msa0693510:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTGTTTATTCAATTTAA+TGG + chr4_4:62451800-62451819 Msa0693510:intron 15.0%
!!! ATTGCTATTTTTGAAATTTT+AGG + chr4_4:62451831-62451850 Msa0693510:intron 15.0%
!!! TTGTTGTTTATTCAATTTAA+TGG + chr4_4:62451800-62451819 Msa0693510:intron 15.0%
!!! ATTGCTATTTTTGAAATTTT+AGG + chr4_4:62451831-62451850 Msa0693510:intron 15.0%
!! TCATGAGTGAAATATAATAT+AGG - chr4_4:62451522-62451541 None:intergenic 20.0%
!!! AAAGATAAATAGCTTTTGTA+AGG + chr4_4:62451576-62451595 Msa0693510:intron 20.0%
!! AAGAAAAAAAATGACCAAAT+GGG - chr4_4:62451640-62451659 None:intergenic 20.0%
!!! CTAAAATCTCTAAAATTGTA+GGG - chr4_4:62451672-62451691 None:intergenic 20.0%
!!! ACTATACTTTTAAAGATGTT+AGG - chr4_4:62451755-62451774 None:intergenic 20.0%
!! TCATGAGTGAAATATAATAT+AGG - chr4_4:62451522-62451541 None:intergenic 20.0%
!!! AAAGATAAATAGCTTTTGTA+AGG + chr4_4:62451576-62451595 Msa0693510:intron 20.0%
!! AAGAAAAAAAATGACCAAAT+GGG - chr4_4:62451640-62451659 None:intergenic 20.0%
!!! CTAAAATCTCTAAAATTGTA+GGG - chr4_4:62451672-62451691 None:intergenic 20.0%
!!! ACTATACTTTTAAAGATGTT+AGG - chr4_4:62451755-62451774 None:intergenic 20.0%
! AAGGAAAAAATGTGTTCTAT+TGG + chr4_4:62451298-62451317 Msa0693510:CDS 25.0%
! TACAGCTAGATATTATCTTT+AGG + chr4_4:62451463-62451482 Msa0693510:intron 25.0%
! ACAGCTAGATATTATCTTTA+GGG + chr4_4:62451464-62451483 Msa0693510:intron 25.0%
!!! ATTATCTTTAGGGTTATTCA+AGG + chr4_4:62451474-62451493 Msa0693510:intron 25.0%
!! TAAATAGCTTTTGTAAGGAT+CGG + chr4_4:62451581-62451600 Msa0693510:intron 25.0%
! AAATAACAATTTAGCCCATT+TGG + chr4_4:62451623-62451642 Msa0693510:intron 25.0%
! GAAGAAAAAAAATGACCAAA+TGG - chr4_4:62451641-62451660 None:intergenic 25.0%
!!! CCTAAAATCTCTAAAATTGT+AGG - chr4_4:62451673-62451692 None:intergenic 25.0%
!!! CCTACAATTTTAGAGATTTT+AGG + chr4_4:62451670-62451689 Msa0693510:intron 25.0%
! TCAATATGATATTCTTGCAT+AGG + chr4_4:62451724-62451743 Msa0693510:intron 25.0%
!! ATGTATATGAGTTGAATTTG+TGG + chr4_4:62451900-62451919 Msa0693510:intron 25.0%
! AAGGAAAAAATGTGTTCTAT+TGG + chr4_4:62451298-62451317 Msa0693510:CDS 25.0%
! TACAGCTAGATATTATCTTT+AGG + chr4_4:62451463-62451482 Msa0693510:intron 25.0%
! ACAGCTAGATATTATCTTTA+GGG + chr4_4:62451464-62451483 Msa0693510:intron 25.0%
!!! ATTATCTTTAGGGTTATTCA+AGG + chr4_4:62451474-62451493 Msa0693510:intron 25.0%
!! TAAATAGCTTTTGTAAGGAT+CGG + chr4_4:62451581-62451600 Msa0693510:intron 25.0%
! AAATAACAATTTAGCCCATT+TGG + chr4_4:62451623-62451642 Msa0693510:intron 25.0%
! GAAGAAAAAAAATGACCAAA+TGG - chr4_4:62451641-62451660 None:intergenic 25.0%
!!! CCTAAAATCTCTAAAATTGT+AGG - chr4_4:62451673-62451692 None:intergenic 25.0%
!!! CCTACAATTTTAGAGATTTT+AGG + chr4_4:62451670-62451689 Msa0693510:intron 25.0%
! TCAATATGATATTCTTGCAT+AGG + chr4_4:62451724-62451743 Msa0693510:intron 25.0%
!! ATGTATATGAGTTGAATTTG+TGG + chr4_4:62451900-62451919 Msa0693510:intron 25.0%
TAGTCCTTGAAGAATTGTAT+AGG + chr4_4:62451202-62451221 Msa0693510:CDS 30.0%
TTGAAGAATTGTATAGGCAA+GGG + chr4_4:62451208-62451227 Msa0693510:CDS 30.0%
! GTTATTCAAGGTTCTTTTAG+TGG + chr4_4:62451486-62451505 Msa0693510:intron 30.0%
ACACACACTTCAAATTGTAA+GGG - chr4_4:62451870-62451889 None:intergenic 30.0%
AGTTAAAGAGACAAAGAAGT+GGG + chr4_4:62451953-62451972 Msa0693510:CDS 30.0%
TAGTCCTTGAAGAATTGTAT+AGG + chr4_4:62451202-62451221 Msa0693510:CDS 30.0%
TTGAAGAATTGTATAGGCAA+GGG + chr4_4:62451208-62451227 Msa0693510:CDS 30.0%
! GTTATTCAAGGTTCTTTTAG+TGG + chr4_4:62451486-62451505 Msa0693510:intron 30.0%
ACACACACTTCAAATTGTAA+GGG - chr4_4:62451870-62451889 None:intergenic 30.0%
AGTTAAAGAGACAAAGAAGT+GGG + chr4_4:62451953-62451972 Msa0693510:CDS 30.0%
ACTAGTAATTGAACTGGTGT+TGG - chr4_4:62451186-62451205 None:intergenic 35.0%
! TCAAGGACTAGTAATTGAAC+TGG - chr4_4:62451192-62451211 None:intergenic 35.0%
CTTGCCTATACAATTCTTCA+AGG - chr4_4:62451209-62451228 None:intergenic 35.0%
CTTGAAGAATTGTATAGGCA+AGG + chr4_4:62451207-62451226 Msa0693510:CDS 35.0%
CGACAATTTGGAAAGATTGA+AGG + chr4_4:62451279-62451298 Msa0693510:CDS 35.0%
! AGAGAAAGAGAAGTACGTTA+TGG + chr4_4:62451410-62451429 Msa0693510:CDS 35.0%
! GAGAAAGAGAAGTACGTTAT+GGG + chr4_4:62451411-62451430 Msa0693510:CDS 35.0%
CACACACACTTCAAATTGTA+AGG - chr4_4:62451871-62451890 None:intergenic 35.0%
! TGAGTTGAATTTGTGGTGAT+TGG + chr4_4:62451907-62451926 Msa0693510:intron 35.0%
!! GATTGGATTTTGAAAGAGAC+AGG + chr4_4:62451924-62451943 Msa0693510:intron 35.0%
GAGTTAAAGAGACAAAGAAG+TGG + chr4_4:62451952-62451971 Msa0693510:CDS 35.0%
ACTAGTAATTGAACTGGTGT+TGG - chr4_4:62451186-62451205 None:intergenic 35.0%
! TCAAGGACTAGTAATTGAAC+TGG - chr4_4:62451192-62451211 None:intergenic 35.0%
CTTGCCTATACAATTCTTCA+AGG - chr4_4:62451209-62451228 None:intergenic 35.0%
CTTGAAGAATTGTATAGGCA+AGG + chr4_4:62451207-62451226 Msa0693510:CDS 35.0%
CGACAATTTGGAAAGATTGA+AGG + chr4_4:62451279-62451298 Msa0693510:CDS 35.0%
! AGAGAAAGAGAAGTACGTTA+TGG + chr4_4:62451410-62451429 Msa0693510:CDS 35.0%
! GAGAAAGAGAAGTACGTTAT+GGG + chr4_4:62451411-62451430 Msa0693510:CDS 35.0%
CACACACACTTCAAATTGTA+AGG - chr4_4:62451871-62451890 None:intergenic 35.0%
! TGAGTTGAATTTGTGGTGAT+TGG + chr4_4:62451907-62451926 Msa0693510:intron 35.0%
!! GATTGGATTTTGAAAGAGAC+AGG + chr4_4:62451924-62451943 Msa0693510:intron 35.0%
GAGTTAAAGAGACAAAGAAG+TGG + chr4_4:62451952-62451971 Msa0693510:CDS 35.0%
! TGTTGGATTTGTTCAGCTGA+TGG - chr4_4:62451243-62451262 None:intergenic 40.0%
!! CTATTGGTTCCAGAATCACA+AGG + chr4_4:62451314-62451333 Msa0693510:CDS 40.0%
CAATTGTTTCCTCCATCTCA+AGG - chr4_4:62451365-62451384 None:intergenic 40.0%
CTTGAGATGGAGGAAACAAT+TGG + chr4_4:62451363-62451382 Msa0693510:CDS 40.0%
!! TGGATTTTGAAAGAGACAGG+TGG + chr4_4:62451927-62451946 Msa0693510:intron 40.0%
!! ATTTTGAAAGAGACAGGTGG+TGG + chr4_4:62451930-62451949 Msa0693510:intron 40.0%
TTGTTCACTGCAGTTTGAAG+TGG - chr4_4:62451982-62452001 None:intergenic 40.0%
! TGTTGGATTTGTTCAGCTGA+TGG - chr4_4:62451243-62451262 None:intergenic 40.0%
!! CTATTGGTTCCAGAATCACA+AGG + chr4_4:62451314-62451333 Msa0693510:CDS 40.0%
CAATTGTTTCCTCCATCTCA+AGG - chr4_4:62451365-62451384 None:intergenic 40.0%
CTTGAGATGGAGGAAACAAT+TGG + chr4_4:62451363-62451382 Msa0693510:CDS 40.0%
!! TGGATTTTGAAAGAGACAGG+TGG + chr4_4:62451927-62451946 Msa0693510:intron 40.0%
!! ATTTTGAAAGAGACAGGTGG+TGG + chr4_4:62451930-62451949 Msa0693510:intron 40.0%
TTGTTCACTGCAGTTTGAAG+TGG - chr4_4:62451982-62452001 None:intergenic 40.0%
TGCATCCTCAACCAAGTTCA+AGG + chr4_4:62451154-62451173 Msa0693510:CDS 45.0%
ATCCTCAACCAAGTTCAAGG+TGG + chr4_4:62451157-62451176 Msa0693510:CDS 45.0%
GAAGCTGCGAAGCTATTTGT+TGG - chr4_4:62451260-62451279 None:intergenic 45.0%
TCAGAAAAGCAAGAAGCTGC+AGG + chr4_4:62451438-62451457 Msa0693510:CDS 45.0%
AAACTGCAGTGAACAAGCAG+AGG + chr4_4:62451986-62452005 Msa0693510:CDS 45.0%
TGCATCCTCAACCAAGTTCA+AGG + chr4_4:62451154-62451173 Msa0693510:CDS 45.0%
ATCCTCAACCAAGTTCAAGG+TGG + chr4_4:62451157-62451176 Msa0693510:CDS 45.0%
GAAGCTGCGAAGCTATTTGT+TGG - chr4_4:62451260-62451279 None:intergenic 45.0%
TCAGAAAAGCAAGAAGCTGC+AGG + chr4_4:62451438-62451457 Msa0693510:CDS 45.0%
AAACTGCAGTGAACAAGCAG+AGG + chr4_4:62451986-62452005 Msa0693510:CDS 45.0%
CTCCACCTTGAACTTGGTTG+AGG - chr4_4:62451162-62451181 None:intergenic 50.0%
GTTGGACTCCACCTTGAACT+TGG - chr4_4:62451168-62451187 None:intergenic 50.0%
GCTTCGCAGCTTCGACAATT+TGG + chr4_4:62451267-62451286 Msa0693510:CDS 50.0%
CTCCACCTTGAACTTGGTTG+AGG - chr4_4:62451162-62451181 None:intergenic 50.0%
GTTGGACTCCACCTTGAACT+TGG - chr4_4:62451168-62451187 None:intergenic 50.0%
GCTTCGCAGCTTCGACAATT+TGG + chr4_4:62451267-62451286 Msa0693510:CDS 50.0%
GCTCTCTCGCCTTGTGATTC+TGG - chr4_4:62451326-62451345 None:intergenic 55.0%
GCTCTCTCGCCTTGTGATTC+TGG - chr4_4:62451326-62451345 None:intergenic 55.0%
!! GAAGCGTCGTCGCCTTGAGA+TGG + chr4_4:62451350-62451369 Msa0693510:CDS 60.0%
!! GAAGCGTCGTCGCCTTGAGA+TGG + chr4_4:62451350-62451369 Msa0693510:CDS 60.0%
GCGTCGTCGCCTTGAGATGG+AGG + chr4_4:62451353-62451372 Msa0693510:CDS 65.0%
GCGTCGTCGCCTTGAGATGG+AGG + chr4_4:62451353-62451372 Msa0693510:CDS 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 62451123 62452034 62451123 ID=Msa0693510;Name=Msa0693510
chr4_4 mRNA 62451123 62452034 62451123 ID=Msa0693510-mRNA-1;Parent=Msa0693510;Name=Msa0693510-mRNA-1;_AED=0.15;_eAED=0.18;_QI=0|0|0|1|1|1|2|0|143
chr4_4 exon 62451123 62451459 62451123 ID=Msa0693510-mRNA-1:exon:17467;Parent=Msa0693510-mRNA-1
chr4_4 exon 62451940 62452034 62451940 ID=Msa0693510-mRNA-1:exon:17468;Parent=Msa0693510-mRNA-1
chr4_4 CDS 62451123 62451459 62451123 ID=Msa0693510-mRNA-1:cds;Parent=Msa0693510-mRNA-1
chr4_4 CDS 62451940 62452034 62451940 ID=Msa0693510-mRNA-1:cds;Parent=Msa0693510-mRNA-1
Gene Sequence

>Msa0693510

CATGCAGCTGAGCAAGGTACAAGCAATACTATGCATCCTCAACCAAGTTCAAGGTGGAGTCCAACACCAGTTCAATTACTAGTCCTTGAAGAATTGTATAGGCAAGGGATGAAAACACCATCAGCTGAACAAATCCAACAAATAGCTTCGCAGCTTCGACAATTTGGAAAGATTGAAGGAAAAAATGTGTTCTATTGGTTCCAGAATCACAAGGCGAGAGAGCGACAGAAGCGTCGTCGCCTTGAGATGGAGGAAACAATTGGTTCTTCTTCAACTGAAGATAAAAAAGAGAAAGAGAAGTACGTTATGGGAAATTCAGAAAAGCAAGAAGCTGCAGAGACAGGTGGTGGAGTTAAAGAGACAAAGAAGTGGGCAACCACTTCAAACTGCAGTGAACAAGCAGAGGTCTGTAATTTCTCTTGTCTTTGTTGA

Protein sequence

>Msa0693510

HAAEQGTSNTMHPQPSSRWSPTPVQLLVLEELYRQGMKTPSAEQIQQIASQLRQFGKIEGKNVFYWFQNHKARERQKRRRLEMEETIGSSSTEDKKEKEKYVMGNSEKQEAAETGGGVKETKKWATTSNCSEQAEVCNFSCLC*