AlfalfaGEDB | Msa0693660

M. sativa cultivar zhongmu-4 / Msa0693660

KEGG: K09260
GO: GO:0000003 GO:0001558 GO:0003002 GO:0003006 GO:0003674 GO:0003700 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009555 GO:0009653 GO:0009791 GO:0009889 GO:0009908 GO:0010016 GO:0010152 GO:0010229 GO:0010468 GO:0010556 GO:0010622 GO:0010769 GO:0019219 GO:0019222 GO:0021700 GO:0022414 GO:0022603 GO:0022604 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0035670 GO:0040008 GO:0043900 GO:0045595 GO:0048229 GO:0048281 GO:0048283 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048444 GO:0048445 GO:0048467 GO:0048481 GO:0048482 GO:0048608 GO:0048638 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051128 GO:0051171 GO:0051239 GO:0051252 GO:0051510 GO:0060255 GO:0060284 GO:0061458 GO:0065007 GO:0080090 GO:0080092 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0099402 GO:0140110 GO:1903506 GO:1905392 GO:2000112 GO:2000241 GO:2001141
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0693660	KEH30865.1	91.228	342	5	4	1	325	1	334	0.0	626

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0693660	sp\|Q9LM46\|AG104_ARATH	49.709	344	137	9	1	317	1	335	3.87e-93	283

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0693660	A0A072UYG7	91.228	342	5	4	1	325	1	334	0.0	626

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TFs/TRs:

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Gene ID	Type	Classification
Msa0693660	TF	MADS-MIKC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0693660	MtrunA17_Chr3g0134451	37.037	270	132	6	1	245	1	257	7.53e-46	158
Msa0693660	MtrunA17_Chr4g0044071	100.000	62	0	0	1	62	1	62	2.98e-37	129
Msa0693660	MtrunA17_Chr5g0425191	93.548	62	4	0	1	62	1	62	8.57e-36	125
Msa0693660	MtrunA17_Chr5g0425181	51.825	137	16	3	128	214	15	151	1.96e-33	121
Msa0693660	MtrunA17_Chr5g0425171	72.500	80	16	4	251	325	1	79	8.46e-24	92.8
Msa0693660	MtrunA17_Chr4g0060841	35.393	178	100	5	1	177	1	164	9.10e-24	100
Msa0693660	MtrunA17_Chr7g0246381	67.241	58	19	0	1	58	1	58	6.61e-23	90.5
Msa0693660	MtrunA17_Chr3g0077501	34.254	181	80	2	1	181	1	142	7.87e-23	94.7
Msa0693660	MtrunA17_Chr3g0097101	34.021	194	88	4	1	194	16	169	8.85e-23	95.1
Msa0693660	MtrunA17_Chr5g0417661	66.129	62	21	0	1	62	56	117	5.05e-22	89.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0693660	AT1G22130.1	49.709	344	137	9	1	317	1	335	3.94e-94	283
Msa0693660	AT1G77980.1	46.802	344	127	7	1	300	1	332	7.76e-90	272
Msa0693660	AT1G77950.3	59.563	183	72	1	1	181	1	183	2.73e-72	224
Msa0693660	AT1G77950.2	59.563	183	72	1	1	181	1	183	2.73e-72	224
Msa0693660	AT1G77950.1	59.563	183	72	1	1	181	1	183	2.73e-72	224
Msa0693660	AT1G77950.4	59.563	183	72	1	1	181	1	183	2.73e-72	224
Msa0693660	AT1G18750.4	44.681	141	64	5	1	140	1	128	8.70e-26	106
Msa0693660	AT1G18750.1	44.681	141	64	5	1	140	1	128	1.01e-25	106
Msa0693660	AT2G03060.3	35.165	182	103	4	1	181	1	168	8.90e-25	103
Msa0693660	AT2G03060.7	35.165	182	103	4	1	181	1	168	8.90e-25	103

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Find 57 sgRNAs with CRISPR-Local

Find 221 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGAACCATATACAGTAG+AGG	0.670428	4_4:-62592924	None:intergenic
AAATGATTAAGACGATTTGA+TGG	0.363568	4_4:-62589189	None:intergenic
AAATGGTAGAGGAGCAAATA+AGG	0.337725	4_4:+62590783	Msa0693660:CDS
AACATTCAAGCATGGCCTCA+AGG	0.560099	4_4:+62593022	Msa0693660:CDS
AATGTTAGTTGGCTGCAAGA+TGG	0.658329	4_4:+62592658	Msa0693660:CDS
ACAACAACTTCAAATGGTAG+AGG	0.593286	4_4:+62590772	Msa0693660:CDS
ACATATTCAAAACGTAGAAA+TGG	0.460629	4_4:+62589099	Msa0693660:CDS
ACGATGGAAACATTCAAGCA+TGG	0.641645	4_4:+62593014	Msa0693660:CDS
AGAAACCTTGGCACGTGTCA+TGG	0.475032	4_4:+62590983	Msa0693660:CDS
AGACATTAACTCTGAGGTTG+AGG	0.553929	4_4:+62590585	Msa0693660:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAGCAATATCAATATCACAA+AGG	-	chr4_4:62589155-62589174	None:intergenic	30.0%
AAATAGATCCTAATCCATAG+AGG	-	chr4_4:62589518-62589537	None:intergenic	30.0%
GAACATACTTATCTCTTTCT+TGG	-	chr4_4:62589737-62589756	None:intergenic	30.0%
TGAAAACTTCTAAACACGTT+TGG	+	chr4_4:62589798-62589817	Msa0693660:intron	30.0%
AGCAGTAGATGAAACAATAT+AGG	-	chr4_4:62590550-62590569	None:intergenic	30.0%
GTAATCTACTAACTTCTTGT+TGG	-	chr4_4:62590751-62590770	None:intergenic	30.0%
GCTATAGATGTCATCTTTAA+TGG	-	chr4_4:62590933-62590952	None:intergenic	30.0%
AAAAAACGTAGTAGAAACCT+TGG	+	chr4_4:62590971-62590990	Msa0693660:CDS	30.0%
TTAATTACCTGAATAGCTGA+TGG	-	chr4_4:62591182-62591201	None:intergenic	30.0%
TGTCACTCAAAATTGAGATT+TGG	+	chr4_4:62591223-62591242	Msa0693660:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	62589042	62593252	62589042	ID=Msa0693660;Name=Msa0693660
chr4_4	mRNA	62589042	62593252	62589042	ID=Msa0693660-mRNA-1;Parent=Msa0693660;Name=Msa0693660-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0\|0\|1\|0.87\|0.88\|9\|0\|325
chr4_4	exon	62589042	62589226	62589042	ID=Msa0693660-mRNA-1:exon:17587;Parent=Msa0693660-mRNA-1
chr4_4	exon	62589695	62589748	62589695	ID=Msa0693660-mRNA-1:exon:17588;Parent=Msa0693660-mRNA-1
chr4_4	exon	62589864	62589896	62589864	ID=Msa0693660-mRNA-1:exon:17589;Parent=Msa0693660-mRNA-1
chr4_4	exon	62590364	62590444	62590364	ID=Msa0693660-mRNA-1:exon:17590;Parent=Msa0693660-mRNA-1
chr4_4	exon	62590579	62590606	62590579	ID=Msa0693660-mRNA-1:exon:17591;Parent=Msa0693660-mRNA-1
chr4_4	exon	62590743	62590804	62590743	ID=Msa0693660-mRNA-1:exon:17592;Parent=Msa0693660-mRNA-1
chr4_4	exon	62590914	62591013	62590914	ID=Msa0693660-mRNA-1:exon:17593;Parent=Msa0693660-mRNA-1
chr4_4	exon	62592637	62592734	62592637	ID=Msa0693660-mRNA-1:exon:17594;Parent=Msa0693660-mRNA-1
chr4_4	exon	62592916	62593252	62592916	ID=Msa0693660-mRNA-1:exon:17595;Parent=Msa0693660-mRNA-1
chr4_4	CDS	62589042	62589226	62589042	ID=Msa0693660-mRNA-1:cds;Parent=Msa0693660-mRNA-1
chr4_4	CDS	62589695	62589748	62589695	ID=Msa0693660-mRNA-1:cds;Parent=Msa0693660-mRNA-1
chr4_4	CDS	62589864	62589896	62589864	ID=Msa0693660-mRNA-1:cds;Parent=Msa0693660-mRNA-1
chr4_4	CDS	62590364	62590444	62590364	ID=Msa0693660-mRNA-1:cds;Parent=Msa0693660-mRNA-1
chr4_4	CDS	62590579	62590606	62590579	ID=Msa0693660-mRNA-1:cds;Parent=Msa0693660-mRNA-1
chr4_4	CDS	62590743	62590804	62590743	ID=Msa0693660-mRNA-1:cds;Parent=Msa0693660-mRNA-1
chr4_4	CDS	62590914	62591013	62590914	ID=Msa0693660-mRNA-1:cds;Parent=Msa0693660-mRNA-1
chr4_4	CDS	62592637	62592734	62592637	ID=Msa0693660-mRNA-1:cds;Parent=Msa0693660-mRNA-1
chr4_4	CDS	62592916	62593252	62592916	ID=Msa0693660-mRNA-1:cds;Parent=Msa0693660-mRNA-1

Gene Sequence

>Msa0693660

ATGGGTCGTGTTAAACTTGAGATAAAAAGAATAGAGAACACAGTGAATCGACAAGTTACATATTCAAAACGTAGAAATGGTCTCATTAAGAAAGCTTATGAGTTATCAATCCTTTGTGATATTGATATTGCTCTTATAATGTTTTCTCCATCAAATCGTCTTAATCATTTTTCAGGTAAAAGAAGGATAGAGGATGTTTTAACTCGTTACATTAATATTCCTGACCAAGAAAGAGATAATGCTGTAAGTTTTCCTGAGCTACCCTACCGAAGTTTATATTGCATTTTGCAGTATTTGCTCAGGATACTTCAGCAGTTGAGGAATGAAAATGACATAGCCCTTCATATGTCCAATTCGGGAGACATTAACTCTGAGGTTGAGGATCTCCAACAAGAAGTTAGTAGATTACAACAACAACTTCAAATGGTAGAGGAGCAAATAAGGAAATATGAACCCGACCCATTAAAGATGACATCTATAGCAGAGCTTGAAACCGGTGAAAAAAACGTAGTAGAAACCTTGGCACGTGTCATGGAGAGAAAGGGGATGCCGACCTCTTTCGAGAATGTTAGTTGGCTGCAAGATGGTGGTCAGAACCAACCCCAAATTTTTGATACATCAGCTCCTATGGATCCTCTCAGGGATTTGTCCTCTACTGTATATGGTTCATTTTCACAAGGGACGAGTTCAAATGTTGTTGATCCACGAGGTATAGGAGAGTGCCACGTGGCAAATCCAAACGATGGAAACATTCAAGCATGGCCTCAAGGATACACATTATATCCTCAACATCAGCAGCATATTCAACATGACATTGTGGGAACTGATCATCATCATCATCACAACATCCAAGATATGATGCTACATGGACAAATGAACATGCCAATCACTGCCTCACAAGTTGAACCTCCCAAAAATGATTCAACAGAATATGATCAATGTAGTAACCAACCTAATCACCAGCTTCATGGTCAATGA

Protein sequence

>Msa0693660

MGRVKLEIKRIENTVNRQVTYSKRRNGLIKKAYELSILCDIDIALIMFSPSNRLNHFSGKRRIEDVLTRYINIPDQERDNAVSFPELPYRSLYCILQYLLRILQQLRNENDIALHMSNSGDINSEVEDLQQEVSRLQQQLQMVEEQIRKYEPDPLKMTSIAELETGEKNVVETLARVMERKGMPTSFENVSWLQDGGQNQPQIFDTSAPMDPLRDLSSTVYGSFSQGTSSNVVDPRGIGECHVANPNDGNIQAWPQGYTLYPQHQQHIQHDIVGTDHHHHHNIQDMMLHGQMNMPITASQVEPPKNDSTEYDQCSNQPNHQLHGQ*