AlfalfaGEDB | Msa0696560

M. sativa cultivar zhongmu-4 / Msa0696560

KEGG: K04733
GO: -
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0696560	GAU46924.1	76.471	374	53	7	6	361	1	357	0.0	552

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0696560	sp\|Q9FIU5\|CRLK1_ARATH	56.870	262	108	3	13	273	8	265	9.77e-96	295
Msa0696560	sp\|Q9FIU5\|CRLK1_ARATH	47.541	61	32	0	258	318	321	381	7.09e-13	72.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0696560	A0A2Z6NRM2	76.471	374	53	7	6	361	1	357	0.0	552

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0696560	MtrunA17_Chr4g0048951	89.531	277	26	1	1	277	1	274	0.0	517
Msa0696560	MtrunA17_Chr4g0048951	94.231	104	6	0	258	361	326	429	2.20e-62	205
Msa0696560	MtrunA17_Chr2g0319101	42.526	388	153	8	27	345	22	408	1.94e-91	281
Msa0696560	MtrunA17_Chr6g0455651	46.923	260	128	6	6	259	1	256	5.36e-67	217
Msa0696560	MtrunA17_Chr4g0044691	51.634	153	74	0	107	259	314	466	9.68e-47	168
Msa0696560	MtrunA17_Chr3g0108361	48.485	165	83	1	97	259	599	763	2.21e-44	163
Msa0696560	MtrunA17_Chr3g0108351	47.239	163	84	1	99	259	599	761	1.21e-42	158
Msa0696560	MtrunA17_Chr5g0432871	47.647	170	84	2	95	259	588	757	1.36e-42	158
Msa0696560	MtrunA17_Chr3g0143961	49.324	148	71	2	108	251	56	203	1.44e-42	152
Msa0696560	MtrunA17_Chr2g0283111	46.196	184	93	3	80	259	571	752	6.52e-42	156

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0696560	AT5G54590.3	56.870	262	108	3	13	273	8	265	9.93e-97	295
Msa0696560	AT5G54590.3	47.541	61	32	0	258	318	321	381	7.21e-14	72.8
Msa0696560	AT5G54590.4	56.870	262	108	3	13	273	8	265	9.93e-97	295
Msa0696560	AT5G54590.4	47.541	61	32	0	258	318	321	381	7.21e-14	72.8
Msa0696560	AT5G54590.2	56.870	262	108	3	13	273	8	265	9.93e-97	295
Msa0696560	AT5G54590.2	47.541	61	32	0	258	318	321	381	7.21e-14	72.8
Msa0696560	AT5G54590.1	58.182	220	90	2	13	231	8	226	1.50e-83	254
Msa0696560	AT5G15730.3	53.061	196	86	3	69	259	62	256	1.45e-63	209
Msa0696560	AT5G15730.2	53.061	196	86	3	69	259	62	256	1.45e-63	209
Msa0696560	AT5G15730.1	52.821	195	86	3	69	259	62	254	7.82e-63	207

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Find 79 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCATGAGCCATTGAGC+TGG	0.533333	4_4:+66529563	Msa0696560:CDS
AAACAGGACCAAATGCTCCA+TGG	0.621902	4_4:-66528719	None:intergenic
AAAGCCTATGCATATTCCTA+TGG	0.531162	4_4:-66528302	None:intergenic
AAAGTGAGGACAAGATTGCA+TGG	0.584798	4_4:+66530731	Msa0696560:CDS
AACACGCCTTTCTGGTTAGA+AGG	0.374544	4_4:+66528517	Msa0696560:CDS
AACATACTTGTAAGAATATT+TGG	0.053872	4_4:-66528578	None:intergenic
AAGAATCTTGTGGGTTTGGT+GGG	0.494345	4_4:+66529292	Msa0696560:CDS
AAGAATGCTGTCTCAGTATG+TGG	0.545605	4_4:+66528550	Msa0696560:CDS
AAGTGAGGACAAGATTGCAT+GGG	0.628499	4_4:+66530732	Msa0696560:CDS
AATCTTGTCCTCACTTTCCA+TGG	0.491517	4_4:-66530725	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCTAATTTCACAACGATCAT+AGG	+	chr4_4:66528696-66528715	Msa0696560:CDS	30.0%
ACAGTTGAAGATCATATTGT+TGG	-	chr4_4:66529151-66529170	None:intergenic	30.0%
GATGTAATCTTCCAAGTAAT+AGG	-	chr4_4:66529270-66529289	None:intergenic	30.0%
CTTTACATTTACATGAGCAA+CGG	+	chr4_4:66529346-66529365	Msa0696560:CDS	30.0%
TTTGTGTGAACTAAATGCAA+AGG	-	chr4_4:66529825-66529844	None:intergenic	30.0%
TAGTTCACACAAAGATGAAT+AGG	+	chr4_4:66529832-66529851	Msa0696560:intron	30.0%
AGTTCACACAAAGATGAATA+GGG	+	chr4_4:66529833-66529852	Msa0696560:intron	30.0%
CATTGTTTACTTCCTCTTAA+AGG	-	chr4_4:66529998-66530017	None:intergenic	30.0%
ACTAGGTATTAATGTCTTCT+CGG	-	chr4_4:66530038-66530057	None:intergenic	30.0%
GCATCATCTTTACAAAAACT+AGG	-	chr4_4:66530055-66530074	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	66528262	66531035	66528262	ID=Msa0696560;Name=Msa0696560
chr4_4	mRNA	66528262	66531035	66528262	ID=Msa0696560-mRNA-1;Parent=Msa0696560;Name=Msa0696560-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0\|0\|0\|1\|1\|1\|6\|0\|361
chr4_4	exon	66528262	66528593	66528262	ID=Msa0696560-mRNA-1:exon:19343;Parent=Msa0696560-mRNA-1
chr4_4	exon	66528677	66528830	66528677	ID=Msa0696560-mRNA-1:exon:19344;Parent=Msa0696560-mRNA-1
chr4_4	exon	66529265	66529391	66529265	ID=Msa0696560-mRNA-1:exon:19345;Parent=Msa0696560-mRNA-1
chr4_4	exon	66529560	66529645	66529560	ID=Msa0696560-mRNA-1:exon:19346;Parent=Msa0696560-mRNA-1
chr4_4	exon	66529742	66529813	66529742	ID=Msa0696560-mRNA-1:exon:19347;Parent=Msa0696560-mRNA-1
chr4_4	exon	66530721	66531035	66530721	ID=Msa0696560-mRNA-1:exon:19348;Parent=Msa0696560-mRNA-1
chr4_4	CDS	66528262	66528593	66528262	ID=Msa0696560-mRNA-1:cds;Parent=Msa0696560-mRNA-1
chr4_4	CDS	66528677	66528830	66528677	ID=Msa0696560-mRNA-1:cds;Parent=Msa0696560-mRNA-1
chr4_4	CDS	66529265	66529391	66529265	ID=Msa0696560-mRNA-1:cds;Parent=Msa0696560-mRNA-1
chr4_4	CDS	66529560	66529645	66529560	ID=Msa0696560-mRNA-1:cds;Parent=Msa0696560-mRNA-1
chr4_4	CDS	66529742	66529813	66529742	ID=Msa0696560-mRNA-1:cds;Parent=Msa0696560-mRNA-1
chr4_4	CDS	66530721	66531035	66530721	ID=Msa0696560-mRNA-1:cds;Parent=Msa0696560-mRNA-1

Gene Sequence

>Msa0696560

ATGAATTCGCACGAGATGAGCAGAACTCTTTTAATTTTGGCCATAGGAATATGCATAGGCTTTGTGATTGGAGCTGGTTTGGTATTTTCAATTTTAGTCTTCTGCAGGCCTGGAAGGAAGCGTGTAGAGGTGGAGAAGAGCGGTCCTCTGAGAACCGAGGCCATTAATGTTCATGGCAAAGGGGCTGATTCCAGTGTAACATCATTATCAGACTCCAACGCGACTTTTGAGTCACCAAGAACATCGGAATGGAGTAACACGCCTTTCTGGTTAGAAGGACTTAGGAGGAAGAATGCTGTCTCAGTATGTGGAATTCCCAAATATTCTTACAAAGATATAGAAAAGGCTACATCTAATTTCACAACGATCATAGGCCATGGAGCATTTGGTCCTGTTTACAAAGCAATCATGCCTACAGGCGAGGCAGTTGCGGTTAAAGTTCTCGGTGCTAATTCAAGACAAGGAGAACAAGAATTTTTAACTGAGGTCCTATTACTTGGAAGATTACATCATAAGAATCTTGTGGGTTTGGTGGGATATGCTGCAGAAAGAGGAAAGCACATGCTTCTTTACATTTACATGAGCAACGGCAGTCTTGCTTCTCATTTGTATGGCGAAAATCATGAGCCATTGAGCTGGAATTTGAGACTTAGCATAGCACTAGATGTTGCAAGGGTGATGGAATATCTACATTATTCGATCGATCCTCCTATCGTGCACCGTGACATCAAGTCTTCAAACATTCTATTAGACAAGTTTATGAAAGCCAAGGCTGCCATGGAAAGTGAGGACAAGATTGCATGGGAAGAAATTGTGGATCCGCAACTGAATGGAAACTATGATGTACATAAGCTTAATGACATGGCTTCACTTGCACTCAAATGTGTCAATGGTGTCTCCAAAATTCGACCTTCAATGAGGAACATTGTTCAAGCATTATCTCAACTTTATAAGAAGCCTAAGAGAAATTCTAGCCGAGTAGCTCCTCCTGCGTTAAATGAAGTGTCTGTTGAAGTGATGCAGCCAGAAACTCGTGACTTTTCTATTGAGTGTTCAGAGGAGCTGCGCAGATTGCACAGCCGATAA

Protein sequence

>Msa0696560

MNSHEMSRTLLILAIGICIGFVIGAGLVFSILVFCRPGRKRVEVEKSGPLRTEAINVHGKGADSSVTSLSDSNATFESPRTSEWSNTPFWLEGLRRKNAVSVCGIPKYSYKDIEKATSNFTTIIGHGAFGPVYKAIMPTGEAVAVKVLGANSRQGEQEFLTEVLLLGRLHHKNLVGLVGYAAERGKHMLLYIYMSNGSLASHLYGENHEPLSWNLRLSIALDVARVMEYLHYSIDPPIVHRDIKSSNILLDKFMKAKAAMESEDKIAWEEIVDPQLNGNYDVHKLNDMASLALKCVNGVSKIRPSMRNIVQALSQLYKKPKRNSSRVAPPALNEVSVEVMQPETRDFSIECSEELRRLHSR*