Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0696980 | XP_024637833.2 | 92.534 | 509 | 29 | 3 | 23 | 530 | 195 | 695 | 0.0 | 964 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0696980 | sp|Q9LXA5|LRK91_ARATH | 53.478 | 460 | 187 | 6 | 27 | 480 | 187 | 625 | 2.04e-165 | 486 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0696980 | A0A396IAL3 | 92.534 | 509 | 29 | 3 | 23 | 530 | 195 | 695 | 0.0 | 964 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0696980 | MtrunA17_Chr4g0049601 | 92.534 | 509 | 29 | 3 | 23 | 530 | 195 | 695 | 0.0 | 964 |
| Msa0696980 | MtrunA17_Chr4g0049581 | 77.119 | 472 | 71 | 5 | 38 | 506 | 228 | 665 | 0.0 | 723 |
| Msa0696980 | MtrunA17_Chr7g0274651 | 54.603 | 478 | 194 | 10 | 18 | 480 | 189 | 658 | 3.12e-174 | 507 |
| Msa0696980 | MtrunA17_Chr8g0367071 | 54.678 | 481 | 184 | 7 | 25 | 496 | 204 | 659 | 3.18e-171 | 499 |
| Msa0696980 | MtrunA17_Chr4g0049551 | 55.338 | 459 | 190 | 6 | 27 | 481 | 202 | 649 | 1.12e-162 | 477 |
| Msa0696980 | MtrunA17_Chr4g0049561 | 50.636 | 472 | 204 | 6 | 27 | 491 | 199 | 648 | 1.05e-161 | 474 |
| Msa0696980 | MtrunA17_Chr7g0274661 | 52.620 | 458 | 193 | 9 | 20 | 465 | 202 | 647 | 8.40e-160 | 470 |
| Msa0696980 | MtrunA17_Chr8g0366991 | 48.577 | 492 | 197 | 6 | 24 | 480 | 289 | 759 | 2.24e-159 | 473 |
| Msa0696980 | MtrunA17_Chr4g0049611 | 49.493 | 493 | 213 | 8 | 18 | 501 | 185 | 650 | 6.60e-159 | 468 |
| Msa0696980 | MtrunA17_Chr8g0366981 | 52.143 | 420 | 179 | 4 | 65 | 480 | 189 | 590 | 2.68e-153 | 451 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0696980 | AT5G10530.1 | 53.478 | 460 | 187 | 6 | 27 | 480 | 187 | 625 | 2.07e-166 | 486 |
| Msa0696980 | AT5G65600.1 | 48.188 | 469 | 206 | 7 | 25 | 480 | 202 | 646 | 2.21e-140 | 421 |
| Msa0696980 | AT5G55830.1 | 42.038 | 471 | 249 | 7 | 25 | 480 | 196 | 657 | 5.73e-117 | 360 |
| Msa0696980 | AT3G53810.1 | 41.463 | 451 | 243 | 7 | 24 | 471 | 196 | 628 | 1.57e-116 | 359 |
| Msa0696980 | AT3G55550.1 | 41.202 | 466 | 244 | 9 | 24 | 483 | 194 | 635 | 1.00e-115 | 357 |
| Msa0696980 | AT4G02410.1 | 39.246 | 451 | 258 | 9 | 24 | 471 | 198 | 635 | 2.68e-109 | 340 |
| Msa0696980 | AT3G53380.1 | 39.920 | 501 | 242 | 11 | 25 | 480 | 183 | 669 | 1.80e-108 | 339 |
| Msa0696980 | AT2G37710.1 | 38.889 | 450 | 255 | 7 | 24 | 471 | 196 | 627 | 1.21e-107 | 336 |
| Msa0696980 | AT5G06740.1 | 41.447 | 456 | 228 | 11 | 52 | 498 | 217 | 642 | 4.53e-106 | 332 |
| Msa0696980 | AT5G06740.2 | 41.319 | 455 | 230 | 9 | 52 | 498 | 229 | 654 | 6.66e-106 | 331 |
Find 111 sgRNAs with CRISPR-Local
Find 248 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCCATAATAAACCTTA+AGG | 0.462701 | 4_4:-66980530 | Msa0696980:CDS |
| AAAAGAAGGGAGGAGGAAGA+TGG | 0.478974 | 4_4:-66981237 | Msa0696980:CDS |
| AAAAGCAAGTAACATCATGT+TGG | 0.549295 | 4_4:-66980746 | Msa0696980:CDS |
| AAACAAAGTTTGTTGAAATG+GGG | 0.580340 | 4_4:-66980856 | Msa0696980:CDS |
| AAAGGGCCGTCAAGATATGT+TGG | 0.593660 | 4_4:+66980265 | None:intergenic |
| AACACAAATGGTTCAGAGGA+AGG | 0.545336 | 4_4:-66980220 | Msa0696980:CDS |
| AAGATGGAGACCTTGTAACT+GGG | 0.614709 | 4_4:+66981373 | None:intergenic |
| AAGCCAATAATAACAAATTC+TGG | 0.340518 | 4_4:+66981501 | None:intergenic |
| AAGCTAGGACAAGGAGGATT+TGG | 0.327241 | 4_4:-66981108 | Msa0696980:CDS |
| AATCTAAGTGTTGTATTCAC+TGG | 0.396170 | 4_4:-66981594 | Msa0696980:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATACATAGCATCTCTTCAT+GGG | - | chr4_4:66980371-66980390 | Msa0696980:CDS | 30.0% | |
| TTTCTTGTGGAAGAAAAGAA+GGG | - | chr4_4:66980572-66980591 | Msa0696980:CDS | 30.0% | |
| TCTGCATATGTATACTTCTT+GGG | + | chr4_4:66980657-66980676 | None:intergenic | 30.0% | |
| TTCTGCATATGTATACTTCT+TGG | + | chr4_4:66980658-66980677 | None:intergenic | 30.0% | |
| AGAAATCTAGTCCAATTGAT+TGG | - | chr4_4:66980864-66980883 | Msa0696980:CDS | 30.0% | |
| TGAATGTCTAAACTTCCATT+TGG | + | chr4_4:66980936-66980955 | None:intergenic | 30.0% | |
| ATATACAATAGCTAAAGGCT+TGG | - | chr4_4:66980995-66981014 | Msa0696980:CDS | 30.0% | |
| AAAAGCAAGTAACATCATGT+TGG | - | chr4_4:66981076-66981095 | Msa0696980:CDS | 30.0% | |
| TGAGCCTTAAGGTTTATTAT+GGG | + | chr4_4:66981299-66981318 | None:intergenic | 30.0% | |
| TTGAGCCTTAAGGTTTATTA+TGG | + | chr4_4:66981300-66981319 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 66980126 | 66981718 | 66980126 | ID=Msa0696980;Name=Msa0696980 |
| chr4_4 | mRNA | 66980126 | 66981718 | 66980126 | ID=Msa0696980-mRNA-1;Parent=Msa0696980;Name=Msa0696980-mRNA-1;_AED=0.19;_eAED=0.21;_QI=0|-1|0|1|-1|1|1|0|530 |
| chr4_4 | exon | 66980126 | 66981718 | 66980126 | ID=Msa0696980-mRNA-1:exon:19573;Parent=Msa0696980-mRNA-1 |
| chr4_4 | CDS | 66980126 | 66981718 | 66980126 | ID=Msa0696980-mRNA-1:cds;Parent=Msa0696980-mRNA-1 |
| Gene Sequence |
>Msa0696980 ATGCCGGAATCGACATCAACTCTATGCTGTCTGTTGCTAATGTTACATGGTTGGCTGATATCAAACAATGAGGCTTGGATCAATTACAATGCTAGTTCATTAAATCTAAGTGTTGTATTCACTGGTTTTAATAATGTAACTTCTAGCGTTGTTAATCAACATTTATCTTCCATAGTTGATCTTAGACTTTATCTGCCAGAATTTGTTATTATTGGCTTCTCAGCTGCCACTGGAAATTCAACTGCTATACATAGCATCTCTTCATGGGATTTTAGCTCAACTTTAGAAGGACAACAAGATAACAACAAAACAAACACACAAGACCCAGTTACAAGGTCTCCATCTTCAAACAAAGCTCCCATTATGAAGAAAAAGGCCATGACAGGACTAGCAGTGGGATTGGGAGCCGGTGGATTCGTTCTGATTTGTGTATTCGTTATTTTGGTTTTCTTGTGGAAGAAAAGAAGGGAGGAGGAAGATGGTGAATTTGAAGAGTACATGGGTGAAGATTTCGGAAGAGGGACAGGACCCAAGAAGTATACATATGCAGAATTAGCACATGGAGCTAATAATTTCAAAGATGAGCATAAGCTAGGACAAGGAGGATTTGGAGGTGTTTATAGGGGTTTTCTTAAAGACGCAAAATCTTATGTTGCTATTAAGAGGGTGTCAGAAGATTCTCATCAAGGGATAAAAGAGTTTGCATCAGAAGTAACGATAATTAGCAAATTAAGGCATAGAAATCTAGTCCAATTGATTGGTTGGTGTCATCAAAGGAAAAAGCTCTTGCTTGTATATGAGTACATGCCAAATGGAAGTTTAGACATTCATCTTTTCAAGAAACAAAGTTTGTTGAAATGGGGAGTTAGATATACAATAGCTAAAGGCTTGGCATCTGCATTGTTGTACTTGCATGAAGAATGGGAACAATGTGTTGTACATAGAGACATAAAAGCAAGTAACATCATGTTGGATTCAAAGTTTAATGCAAAACTTGGAGATTTTGGTTTAGCAAGGTTTGTGGACCATGCAAAAGGTGCACAAACTACAGCTTTAGCAGGGACTTTGGGATACATGGCTCCAGAATGTGCTACCACAGGAAGAGCTAGTAAAGAAACAGATGTGTATAGTTTTGGAATAGTTACTTTAGAGATAGCTTGTGGAAGAAAACCCATAATAAACCTTAAGGCTCAAGAAAATGAAATACATATTGTTGAGTGGGTTTGGGGGCTTTATGGAAGAGGGAGAATTGTTGAAGCAGTAGATCCAAGACTAGATGGAGATTTTGAAGAGGAGCAAATCAAGTGTATGATGATTGTTGGTCTTTGGTGTGCTCATCCTGATCCTAATAATAGGCCTTCAATAAGACAAGCTATTCAAGCGCTTAATTTTGAAGTTCCATTACCTAATCTTCCATCAAGTATGCCTGTACCAACATATCTTGACGGCCCTTTGAATTCATTCACTGTTCCATTCAACACAAATGGTTCAGAGGAAGGTCAAAACATGAATATAGAAAGCTTTAGTTCGAACACAAACTCATCTGGTTTGATTACTACAACTACTTCTGATGATGTTTCTCCTTCTATGTGA |
| Protein sequence |
>Msa0696980 MPESTSTLCCLLLMLHGWLISNNEAWINYNASSLNLSVVFTGFNNVTSSVVNQHLSSIVDLRLYLPEFVIIGFSAATGNSTAIHSISSWDFSSTLEGQQDNNKTNTQDPVTRSPSSNKAPIMKKKAMTGLAVGLGAGGFVLICVFVILVFLWKKRREEEDGEFEEYMGEDFGRGTGPKKYTYAELAHGANNFKDEHKLGQGGFGGVYRGFLKDAKSYVAIKRVSEDSHQGIKEFASEVTIISKLRHRNLVQLIGWCHQRKKLLLVYEYMPNGSLDIHLFKKQSLLKWGVRYTIAKGLASALLYLHEEWEQCVVHRDIKASNIMLDSKFNAKLGDFGLARFVDHAKGAQTTALAGTLGYMAPECATTGRASKETDVYSFGIVTLEIACGRKPIINLKAQENEIHIVEWVWGLYGRGRIVEAVDPRLDGDFEEEQIKCMMIVGLWCAHPDPNNRPSIRQAIQALNFEVPLPNLPSSMPVPTYLDGPLNSFTVPFNTNGSEEGQNMNIESFSSNTNSSGLITTTTSDDVSPSM* |
