AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0699340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0699340 MtrunA17_Chr8g0375251 96.907 679 21 0 1 679 93 771 0.0 1369
Msa0699340 MtrunA17_Chr4g0063461 57.851 121 34 1 25 145 7 110 9.38e-43 163
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0699340 AT2G02160.1 39.872 627 284 28 3 596 5 571 5.94e-105 334

Find 215 sgRNAs with CRISPR-Local

Find 303 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAGTCCTTCTTTCTATT+TGG 0.122370 4_4:-69982221 None:intergenic
ATTTGGGCTGATCCCTTTCT+AGG 0.173898 4_4:-69982204 None:intergenic
GAGAGAGCCGCCACAGAAAT+TGG 0.190492 4_4:-69979914 None:intergenic
GATCCATCGATCTCTTCTTC+TGG 0.192710 4_4:+69979977 Msa0699340:five_prime_UTR
GAATGTGATGATGGGCTTTA+AGG 0.209581 4_4:-69979822 None:intergenic
AGAATTTCACTAAGTGGTTT+TGG 0.245906 4_4:-69982795 None:intergenic
TATTAACTCTCTAACGTTAC+AGG 0.259234 4_4:-69983419 None:intergenic
TATCATGGGGAAGATGAATT+TGG 0.267482 4_4:+69982018 Msa0699340:CDS
GCTTGTTTACTACCATTATA+AGG 0.280011 4_4:-69981484 None:intergenic
TTTCTCAGAGAGTCGTCTCC+TGG 0.281678 4_4:+69981952 Msa0699340:CDS
CCTCTTCTCAGATTGAGATT+TGG 0.282214 4_4:+69979955 Msa0699340:five_prime_UTR
AGTCTTGCTGAACTTAAGTA+TGG 0.284200 4_4:+69982678 Msa0699340:CDS
ATGAGTCCTTCTTTCTATTT+GGG 0.297001 4_4:-69982220 None:intergenic
TTCTCCCTTGACATGCAAAA+AGG 0.299512 4_4:+69980119 Msa0699340:CDS
TCCTCTTTCCAAATGATTGT+TGG 0.310747 4_4:-69982722 None:intergenic
ATCCATCGATCTCTTCTTCT+GGG 0.318828 4_4:+69979978 Msa0699340:five_prime_UTR
TTGGAAAGAGGAAAAGCTTC+AGG 0.319306 4_4:+69982733 Msa0699340:CDS
ATCGATCTCTTCTTCTGGGT+TGG 0.324812 4_4:+69979982 Msa0699340:five_prime_UTR
CAGCCTCTTGACGGGTTGTT+AGG 0.337201 4_4:+69981379 Msa0699340:intron
CAACACCTGCAACATTTGCT+TGG 0.342566 4_4:-69981705 None:intergenic
CCAAAAGGTTTCTTAAAACT+TGG 0.344005 4_4:-69981646 None:intergenic
TCGATCTCTTCTTCTGGGTT+GGG 0.344716 4_4:+69979983 Msa0699340:five_prime_UTR
AAGAACAACACCAATCTTCT+TGG 0.345542 4_4:-69983156 None:intergenic
ACCCATGCACCGCCTTATAA+TGG 0.352929 4_4:+69981472 Msa0699340:CDS
GGAAGAAGAGTCCAAGATTC+AGG 0.353435 4_4:+69982926 Msa0699340:CDS
CCGGCAACTGCTGCTGCTGC+TGG 0.354940 4_4:+69981409 Msa0699340:CDS
GCCGTTTCCCTTCCTGCGCT+TGG 0.358186 4_4:-69982298 None:intergenic
ATCAGTGGCCGGTTGTCATC+TGG 0.358804 4_4:+69982531 Msa0699340:CDS
TCGTATTCATTCAAAGAGTC+TGG 0.359110 4_4:-69982069 None:intergenic
ATCTCTCATTTGAAGGTAAC+TGG 0.359901 4_4:-69982383 None:intergenic
AGAAATTCTGATTACTATCA+TGG 0.361394 4_4:+69982003 Msa0699340:CDS
AGAGTAAGAGAATGTGATGA+TGG 0.369394 4_4:-69979831 None:intergenic
TTAAGCATGTAGGCCTCAAC+TGG 0.379353 4_4:+69983277 Msa0699340:three_prime_UTR
TTACTGCCATTGGTAGGGTT+TGG 0.386685 4_4:-69981574 None:intergenic
AATAATGACCAACAATCATT+TGG 0.389220 4_4:+69982714 Msa0699340:CDS
CGTCATTGTCCTTACCACTA+TGG 0.395917 4_4:-69981927 None:intergenic
ATCCTAGAGATTGTTGGTAT+TGG 0.401206 4_4:+69981181 Msa0699340:CDS
TTTCTAGGTTCCCAGCCATA+TGG 0.401770 4_4:-69982189 None:intergenic
TTGACAGATCTCTCATTTGA+AGG 0.402339 4_4:-69982390 None:intergenic
ATCATATGAGTCATAACCTT+GGG 0.402504 4_4:-69982154 None:intergenic
TCCATCAGCAGCCTGAATCT+TGG 0.402576 4_4:-69982937 None:intergenic
CTATGGAAATTGTGATCATC+TGG 0.404436 4_4:-69981910 None:intergenic
AAGTTTCTCCTCTCGTCATC+TGG 0.405913 4_4:-69982609 None:intergenic
CGATGAAGCTGCCGAAGATC+AGG 0.408429 4_4:+69983013 Msa0699340:CDS
AAATCAAGAGCAAGAATATA+TGG 0.412298 4_4:+69983109 Msa0699340:CDS
GTACATCATCTTCATATTGT+TGG 0.423888 4_4:-69982761 None:intergenic
TAAAACAAGTAAGCGTGGTT+TGG 0.434567 4_4:-69979799 None:intergenic
AGGATCCAGGATAGATTAAG+AGG 0.436261 4_4:+69982579 Msa0699340:CDS
ACTTCCCAAGCAAATGTTGC+AGG 0.436467 4_4:+69981700 Msa0699340:CDS
ACCTTCATACTCCTGATCTT+CGG 0.437886 4_4:-69983024 None:intergenic
GAAGATGATGTACCATTTGA+AGG 0.438650 4_4:+69982771 Msa0699340:CDS
GAATCTGAAGTTGTGGCTGT+TGG 0.444668 4_4:-69980023 None:intergenic
ACCTTCCTCTTAATCTATCC+TGG 0.445108 4_4:-69982584 None:intergenic
AACACCTGCAACATTTGCTT+GGG 0.445165 4_4:-69981704 None:intergenic
TCCTTCAGAATTTCACTAAG+TGG 0.448374 4_4:-69982801 None:intergenic
AGGTGACTGTCACCACCATT+TGG 0.450256 4_4:-69982444 None:intergenic
GAAATTCTGATTACTATCAT+GGG 0.451701 4_4:+69982004 Msa0699340:CDS
GTCCCTCTCTAGGTGACTGC+TGG 0.452700 4_4:-69982493 None:intergenic
ACACGATTAGACCGGGCATT+AGG 0.453044 4_4:-69981871 None:intergenic
GATTGTTGGTATTGGTTGAA+TGG 0.456384 4_4:+69981189 Msa0699340:CDS
ATCTGTGGCGTGGGGACAGA+TGG 0.457178 4_4:-69981433 None:intergenic
TAAGTTAAAGAGACAATATA+AGG 0.459796 4_4:-69983239 None:intergenic
ATATGGCTGGGAACCTAGAA+AGG 0.461958 4_4:+69982191 Msa0699340:CDS
CGGATGACGGAATGAACACT+TGG 0.466296 4_4:-69981226 None:intergenic
CTCAAGATGAAGCCTTGAAA+AGG 0.468804 4_4:+69980070 Msa0699340:CDS
AATATATGGAAGATGAAGAT+GGG 0.468862 4_4:+69983123 Msa0699340:CDS
AGAGAGCCGCCACAGAAATT+GGG 0.468937 4_4:-69979913 None:intergenic
AACTTGGATTCTCAGCTCCT+TGG 0.469416 4_4:-69981630 None:intergenic
AATCTGAAGTTGTGGCTGTT+GGG 0.469633 4_4:-69980022 None:intergenic
TGACTCTAGCATACTCACTA+TGG 0.470886 4_4:-69981158 None:intergenic
TCTGAGACCTCAACACTGTC+AGG 0.480290 4_4:-69982258 None:intergenic
GCATTAGGCCCATTGGTTGG+CGG 0.480720 4_4:-69981856 None:intergenic
AGAATAAATCTTCCACCAAA+TGG 0.481461 4_4:+69982429 Msa0699340:CDS
AATGAAGGAATTACCGAAAA+CGG 0.483376 4_4:+69982885 Msa0699340:CDS
CATGTACCGCCACCACCTGC+AGG 0.483658 4_4:+69981790 Msa0699340:CDS
GATGACTTTGCCAAGAAGAT+TGG 0.484215 4_4:+69983146 Msa0699340:CDS
GCCTCATGAGGGAAGGATCC+AGG 0.484648 4_4:+69982566 Msa0699340:CDS
TACCATTATAAGGCGGTGCA+TGG 0.485504 4_4:-69981474 None:intergenic
CAACCAATGGGCCTAATGCC+CGG 0.485721 4_4:+69981860 Msa0699340:CDS
TTAGGCCCATTGGTTGGCGG+TGG 0.486496 4_4:-69981853 None:intergenic
TTCTCAGAGAGTCGTCTCCT+GGG 0.488365 4_4:+69981953 Msa0699340:CDS
GAAATTCTGAAGGAGAAGAA+AGG 0.491627 4_4:+69982810 Msa0699340:CDS
TCCAAGCGCAGGAAGGGAAA+CGG 0.492405 4_4:+69982297 Msa0699340:CDS
GAATATATGGAAGATGAAGA+TGG 0.492589 4_4:+69983122 Msa0699340:CDS
CAAGTGTTCATTCCGTCATC+CGG 0.493411 4_4:+69981227 Msa0699340:CDS
TTTACATGCAGCCTCTTGAC+GGG 0.494749 4_4:+69981371 Msa0699340:intron
GTCATCATGTTTGCTACTGT+GGG 0.496484 4_4:-69982856 None:intergenic
TGGATTCTCAGCTCCTTGGT+TGG 0.496891 4_4:-69981626 None:intergenic
TGGTAGGGTTTGGTCCATGC+AGG 0.497954 4_4:-69981564 None:intergenic
TCCAAGATTCAGGCTGCTGA+TGG 0.498699 4_4:+69982936 Msa0699340:CDS
GAAGCCTTCCAGATGACAAC+CGG 0.499100 4_4:-69982539 None:intergenic
CTGGGTTGGGTTGTTCGCCA+TGG 0.501508 4_4:+69979996 Msa0699340:exon
AAGGCTTCATCTTGAGGAGA+AGG 0.502963 4_4:-69980063 None:intergenic
TTATCTAACCCTGCAGGTGG+TGG 0.503622 4_4:-69981799 None:intergenic
TCATCTTGAGGAGAAGGTGT+TGG 0.504643 4_4:-69980057 None:intergenic
TGGAAAGAGGAAAAGCTTCA+GGG 0.504669 4_4:+69982734 Msa0699340:CDS
CTGAAGGAGAAGAAAGGAGT+TGG 0.505461 4_4:+69982816 Msa0699340:CDS
GTATGAGTATGAACAAGTTG+AGG 0.507239 4_4:+69983082 Msa0699340:CDS
GAGAAGAGGAGAAGAAGTAG+AGG 0.508002 4_4:-69979941 None:intergenic
AGTTCTCCACCGCCAACCAA+TGG 0.509937 4_4:+69981847 Msa0699340:CDS
TCTTTGAATGAATACGATGT+AGG 0.510886 4_4:+69982075 Msa0699340:CDS
AAAGGAGTTGGAGCTGGTGC+AGG 0.510993 4_4:+69982828 Msa0699340:CDS
GTTCTCCACCGCCAACCAAT+GGG 0.511996 4_4:+69981848 Msa0699340:CDS
CATGATGACAATAAGAATGA+AGG 0.513449 4_4:+69982870 Msa0699340:CDS
GAGTAAGAGAATGTGATGAT+GGG 0.516605 4_4:-69979830 None:intergenic
AGACCGGGCATTAGGCCCAT+TGG 0.518925 4_4:-69981863 None:intergenic
ACCAACAATCATTTGGAAAG+AGG 0.519008 4_4:+69982721 Msa0699340:CDS
TGTCATCATGTTTGCTACTG+TGG 0.520700 4_4:-69982857 None:intergenic
TCTAACCCTGCAGGTGGTGG+CGG 0.521071 4_4:-69981796 None:intergenic
CTTTGAATGAATACGATGTA+GGG 0.521877 4_4:+69982076 Msa0699340:CDS
CGGAACATTGTCAGAGAGCA+AGG 0.523049 4_4:+69982905 Msa0699340:CDS
GATCACATGCAAGAGCCATA+TGG 0.523697 4_4:+69982174 Msa0699340:CDS
GAGTCAATCCTAGAGATTGT+TGG 0.524919 4_4:+69981175 Msa0699340:CDS
TATGAGTATGAACAAGTTGA+GGG 0.525313 4_4:+69983083 Msa0699340:CDS
GTTGCCGCAATCTGTGGCGT+GGG 0.527866 4_4:-69981442 None:intergenic
ACATGCAAGAGCCATATGGC+TGG 0.534478 4_4:+69982178 Msa0699340:CDS
GCAGCAGCAGCAGTTGCCGG+TGG 0.534503 4_4:-69981406 None:intergenic
GCCTCATTATCTAACCCTGC+AGG 0.535034 4_4:-69981805 None:intergenic
GTGGAAGATTTATTCTGCCT+CGG 0.535587 4_4:-69982422 None:intergenic
ACCTGCAGGGTTAGATAATG+AGG 0.535845 4_4:+69981804 Msa0699340:CDS
GCTTCCATCATATCAACTAA+AGG 0.541333 4_4:-69983469 None:intergenic
GAGAAGAAAGGAGTTGGAGC+TGG 0.544404 4_4:+69982822 Msa0699340:CDS
CTCTGTCCAAGTCCCTCTCT+AGG 0.544981 4_4:-69982503 None:intergenic
CGTTTATCCAAGCGCAGGAA+GGG 0.547030 4_4:+69982291 Msa0699340:CDS
ACCATTATAAGGCGGTGCAT+GGG 0.548023 4_4:-69981473 None:intergenic
GATGATCACAATTTCCATAG+TGG 0.554285 4_4:+69981913 Msa0699340:CDS
TGGCAGTAAAATAGCTACAC+AGG 0.556312 4_4:+69981588 Msa0699340:CDS
AGCAAGAAAGTAGACACAAT+CGG 0.559051 4_4:-69980097 None:intergenic
GATCGAATGAGGACCGAAGT+GGG 0.559665 4_4:+69982640 Msa0699340:CDS
TCCAGGATAGATTAAGAGGA+AGG 0.562641 4_4:+69982583 Msa0699340:CDS
CGGGCATTAGGCCCATTGGT+TGG 0.564557 4_4:-69981859 None:intergenic
CTCTGTCTAAGTCCCTCTCT+AGG 0.564616 4_4:-69982464 None:intergenic
TCCTGGATCCTTCCCTCATG+AGG 0.568928 4_4:-69982567 None:intergenic
GAACCAGCAGTCACCTAGAG+AGG 0.569215 4_4:+69982490 Msa0699340:CDS
TGTCCCCACGCCACAGATTG+CGG 0.569632 4_4:+69981438 Msa0699340:CDS
TGACTGTCACCACCATTTGG+TGG 0.569677 4_4:-69982441 None:intergenic
CGTATTCATTCAAAGAGTCT+GGG 0.569875 4_4:-69982068 None:intergenic
GACAGATGTGCCTTCCTGCA+TGG 0.570126 4_4:+69981550 Msa0699340:CDS
CCAAATCTCAATCTGAGAAG+AGG 0.573248 4_4:-69979955 None:intergenic
TTGAAAGAAAATGCAAGGCA+CGG 0.577753 4_4:-69981506 None:intergenic
CTTCAGCAGTCGATCGAATG+AGG 0.577765 4_4:+69982629 Msa0699340:CDS
TGTTGCCGCAATCTGTGGCG+TGG 0.581148 4_4:-69981443 None:intergenic
GACAGATATTACCCCAGTTG+AGG 0.582522 4_4:-69983290 None:intergenic
GCAGATGTTGCCGCAATCTG+TGG 0.582954 4_4:-69981448 None:intergenic
TCGTTTATCCAAGCGCAGGA+AGG 0.583309 4_4:+69982290 Msa0699340:CDS
GATCAGCCCAAATAGAAAGA+AGG 0.583430 4_4:+69982214 Msa0699340:CDS
GATGAATTTGGTAAAACAAG+AGG 0.583764 4_4:+69982030 Msa0699340:CDS
TCACAATTTCCATAGTGGTA+AGG 0.586818 4_4:+69981918 Msa0699340:CDS
TTGTGGCTGTTGGGCAACCA+TGG 0.587839 4_4:-69980013 None:intergenic
TCGTAGTCCTGACAGTGTTG+AGG 0.587874 4_4:+69982251 Msa0699340:CDS
GTTGATGCACACGATTAGAC+CGG 0.589529 4_4:-69981879 None:intergenic
TTCAATCGCCTCATGAGGGA+AGG 0.590666 4_4:+69982559 Msa0699340:CDS
GATCATATGAGTCATAACCT+TGG 0.590754 4_4:-69982155 None:intergenic
CAGTCACCTAGAGAGGGACT+TGG 0.591478 4_4:+69982497 Msa0699340:CDS
AAGGCTTCAATCGCCTCATG+AGG 0.592807 4_4:+69982554 Msa0699340:CDS
ATCTGTCGTCGCTCATGATG+AGG 0.592954 4_4:+69982326 Msa0699340:CDS
ACCACTTAGTGAAATTCTGA+AGG 0.593072 4_4:+69982800 Msa0699340:CDS
AACCAATACCAACAATCTCT+AGG 0.593370 4_4:-69981183 None:intergenic
CGATCGAATGAGGACCGAAG+TGG 0.593485 4_4:+69982639 Msa0699340:CDS
GACATCGTTTATCCAAGCGC+AGG 0.594290 4_4:+69982286 Msa0699340:CDS
GTCTTGCTGAACTTAAGTAT+GGG 0.596178 4_4:+69982679 Msa0699340:CDS
GATCAGGAGTATGAAGGTGA+AGG 0.597456 4_4:+69983029 Msa0699340:CDS
GCCGAAGATCAGGAGTATGA+AGG 0.599647 4_4:+69983023 Msa0699340:CDS
TCTCTACCCAATTTCTGTGG+CGG 0.599837 4_4:+69979907 Msa0699340:five_prime_UTR
CATGCAAGAGCCATATGGCT+GGG 0.599944 4_4:+69982179 Msa0699340:CDS
CACGGTCAATCATCTGAGGA+AGG 0.602447 4_4:+69982981 Msa0699340:CDS
TAAAGAAACTCACCGGATGA+CGG 0.603837 4_4:-69981239 None:intergenic
CTAGAGAGGGACTTGGACAG+AGG 0.604960 4_4:+69982504 Msa0699340:CDS
CGGGTTGTTAGGAACACCAC+CGG 0.605352 4_4:+69981390 Msa0699340:CDS
TGAATATGAGCAAGGTGATG+AGG 0.606447 4_4:+69983058 Msa0699340:CDS
GTTCCTAACAACCCGTCAAG+AGG 0.607813 4_4:-69981382 None:intergenic
TATGGCTGGGAACCTAGAAA+GGG 0.609578 4_4:+69982192 Msa0699340:CDS
ATCATGTTTGCTACTGTGGG+AGG 0.610033 4_4:-69982853 None:intergenic
GATAGATTTCGTGTGCCCCA+AGG 0.610131 4_4:+69982138 Msa0699340:CDS
ACAAGAGGTCAAGATGAAAG+AGG 0.612476 4_4:+69982045 Msa0699340:CDS
ATATATGGAAGATGAAGATG+GGG 0.612975 4_4:+69983124 Msa0699340:CDS
CTCTCTCTACCCAATTTCTG+TGG 0.614772 4_4:+69979904 Msa0699340:five_prime_UTR
TAAGCATGTAGGCCTCAACT+GGG 0.615366 4_4:+69983278 Msa0699340:three_prime_UTR
TTGCTGAACTTAAGTATGGG+AGG 0.621411 4_4:+69982682 Msa0699340:CDS
GACAATACTGATGCAACTCA+CGG 0.622935 4_4:+69982963 Msa0699340:CDS
GTGGTGACAGTCACCTAGAG+AGG 0.623462 4_4:+69982451 Msa0699340:CDS
ACAGAGGAAGAATCAGTGGC+CGG 0.626828 4_4:+69982520 Msa0699340:CDS
TTGTGATCATCTGGCAAACG+AGG 0.626895 4_4:-69981901 None:intergenic
TTGGCGCAGAGAACCCACTT+CGG 0.629325 4_4:-69982653 None:intergenic
GGCAGTAAAATAGCTACACA+GGG 0.630874 4_4:+69981589 Msa0699340:CDS
AGGGTTTGGTCCATGCAGGA+AGG 0.631683 4_4:-69981560 None:intergenic
AACCCAGAAGAAGAGATCGA+TGG 0.631981 4_4:-69979980 None:intergenic
AACCAGCAGTCACCTAGAGA+GGG 0.636906 4_4:+69982491 Msa0699340:CDS
TAAGGTAAAACAAGTAAGCG+TGG 0.639199 4_4:-69979804 None:intergenic
GAAGGTTGCCAGATGACGAG+AGG 0.639411 4_4:+69982601 Msa0699340:CDS
CTAGAGAGGGACTTAGACAG+AGG 0.639929 4_4:+69982465 Msa0699340:CDS
GGATGACGGAATGAACACTT+GGG 0.640470 4_4:-69981225 None:intergenic
AGGCTTCAATCGCCTCATGA+GGG 0.642774 4_4:+69982555 Msa0699340:CDS
ATGTACCGCCACCACCTGCA+GGG 0.643731 4_4:+69981791 Msa0699340:CDS
AAATTCTGATTACTATCATG+GGG 0.645440 4_4:+69982005 Msa0699340:CDS
TTGCCGCAATCTGTGGCGTG+GGG 0.646254 4_4:-69981441 None:intergenic
GTGGCCGGTTGTCATCTGGA+AGG 0.646666 4_4:+69982535 Msa0699340:CDS
CATGGACCAAACCCTACCAA+TGG 0.648418 4_4:+69981568 Msa0699340:CDS
ATAAATCTTCCACCAAATGG+TGG 0.648459 4_4:+69982432 Msa0699340:CDS
TTGGACAGAGGAAGAATCAG+TGG 0.649407 4_4:+69982516 Msa0699340:CDS
GGAGACTATGAATATGAGCA+AGG 0.650461 4_4:+69983050 Msa0699340:CDS
TTGATGCACACGATTAGACC+GGG 0.654044 4_4:-69981878 None:intergenic
TCTGTCAATAATCGTTTCCG+AGG 0.654101 4_4:+69982405 Msa0699340:CDS
TGCGCTTGGATAAACGATGT+CGG 0.656360 4_4:-69982284 None:intergenic
AACTCACGGTCAATCATCTG+AGG 0.660605 4_4:+69982977 Msa0699340:CDS
TCAATGACAATGACCAACCA+AGG 0.673022 4_4:+69981613 Msa0699340:CDS
TGAGTCTGAATCTGAAGTTG+TGG 0.677604 4_4:-69980030 None:intergenic
TGGTGACAGTCACCTAGAGA+GGG 0.684816 4_4:+69982452 Msa0699340:CDS
TCATTATCTAACCCTGCAGG+TGG 0.685323 4_4:-69981802 None:intergenic
TGTTTACTACCATTATAAGG+CGG 0.686484 4_4:-69981481 None:intergenic
TCATATGAGTCATAACCTTG+GGG 0.704228 4_4:-69982153 None:intergenic
CTCTAGCATACTCACTATGG+CGG 0.707134 4_4:-69981155 None:intergenic
GCTACAAGAACATCAAACCC+AGG 0.714968 4_4:-69981970 None:intergenic
AAGCATGTAGGCCTCAACTG+GGG 0.762858 4_4:+69983279 Msa0699340:three_prime_UTR
GCAGTAAAATAGCTACACAG+GGG 0.802739 4_4:+69981590 Msa0699340:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATTTTAATAATAATA+GGG - chr4_4:69980773-69980792 None:intergenic 0.0%
!! TAATTGATAAAGAAAAAAAA+GGG - chr4_4:69980176-69980195 None:intergenic 10.0%
!! ATAATTGATAAAGAAAAAAA+AGG - chr4_4:69980177-69980196 None:intergenic 10.0%
!!! TTTATCAATTATATCTTTTA+GGG + chr4_4:69980185-69980204 Msa0699340:intron 10.0%
!!! AAATTTAATTTTGATTAATG+GGG + chr4_4:69980267-69980286 Msa0699340:intron 10.0%
!!! CTTTATCAATTATATCTTTT+AGG + chr4_4:69980184-69980203 Msa0699340:intron 15.0%
!! CATAACTCAATTAAAATAAA+AGG - chr4_4:69981283-69981302 None:intergenic 15.0%
!!! TTGTTCTTTCATAATTTTTA+TGG + chr4_4:69983171-69983190 Msa0699340:exon 15.0%
!!! TCTACAATTCAACAATTTTA+GGG - chr4_4:69980329-69980348 None:intergenic 20.0%
!!! ATCTACAATTCAACAATTTT+AGG - chr4_4:69980330-69980349 None:intergenic 20.0%
!! AATACTCTAAACTTAATGAA+AGG - chr4_4:69980682-69980701 None:intergenic 20.0%
!! CATCTGTTATTAAGAATATA+AGG + chr4_4:69981033-69981052 Msa0699340:intron 20.0%
!!! AACTTAAACATATTTTTCTG+TGG - chr4_4:69981763-69981782 None:intergenic 20.0%
!! ACAGAAAAATATGTTTAAGT+TGG + chr4_4:69981762-69981781 Msa0699340:CDS 20.0%
!! TAAGTTAAAGAGACAATATA+AGG - chr4_4:69983242-69983261 None:intergenic 20.0%
!!! TGATATTTTTATGTAAGAAG+TGG + chr4_4:69983363-69983382 Msa0699340:three_prime_UTR 20.0%
!!! GATATTTTTATGTAAGAAGT+GGG + chr4_4:69983364-69983383 Msa0699340:three_prime_UTR 20.0%
!!! ATATTTTTATGTAAGAAGTG+GGG + chr4_4:69983365-69983384 Msa0699340:three_prime_UTR 20.0%
!!! AAAAAGGGCTTTTTAGAAAA+AGG - chr4_4:69980161-69980180 None:intergenic 25.0%
!!! TTTCAGATTCGAATTAGTTT+TGG + chr4_4:69980208-69980227 Msa0699340:intron 25.0%
!!! TAATTTTGATTAATGGGGTT+TGG + chr4_4:69980272-69980291 Msa0699340:intron 25.0%
! ACAAAAAAATCTTCATGGAT+GGG - chr4_4:69980355-69980374 None:intergenic 25.0%
! AACAAAAAAATCTTCATGGA+TGG - chr4_4:69980356-69980375 None:intergenic 25.0%
! GTCTAACAAAAAAATCTTCA+TGG - chr4_4:69980360-69980379 None:intergenic 25.0%
!!! GTTAGACTGTGAATTTTTTA+TGG + chr4_4:69980373-69980392 Msa0699340:intron 25.0%
!!! TGATCATCTTGTTATTTGAT+TGG + chr4_4:69980423-69980442 Msa0699340:intron 25.0%
!! TCCTTTTGTATTTAACATAG+TGG + chr4_4:69980483-69980502 Msa0699340:intron 25.0%
!! CCTTTTGTATTTAACATAGT+GGG + chr4_4:69980484-69980503 Msa0699340:intron 25.0%
! AATAAGCTATTTATCCAAAC+AGG + chr4_4:69980746-69980765 Msa0699340:intron 25.0%
! ATAAGCTATTTATCCAAACA+GGG + chr4_4:69980747-69980766 Msa0699340:intron 25.0%
! AGTATTTGAGTCTTGATTAT+AGG + chr4_4:69980805-69980824 Msa0699340:intron 25.0%
! TTCTCTTCTTTATTGAAACA+GGG + chr4_4:69981117-69981136 Msa0699340:intron 25.0%
! AGAAATTCTGATTACTATCA+TGG + chr4_4:69982003-69982022 Msa0699340:CDS 25.0%
! GAAATTCTGATTACTATCAT+GGG + chr4_4:69982004-69982023 Msa0699340:CDS 25.0%
! AAATTCTGATTACTATCATG+GGG + chr4_4:69982005-69982024 Msa0699340:CDS 25.0%
! AATAATGACCAACAATCATT+TGG + chr4_4:69982714-69982733 Msa0699340:CDS 25.0%
! AAATCAAGAGCAAGAATATA+TGG + chr4_4:69983109-69983128 Msa0699340:CDS 25.0%
! AATATATGGAAGATGAAGAT+GGG + chr4_4:69983123-69983142 Msa0699340:CDS 25.0%
!!! TTTTCCTTTAGTTGATATGA+TGG + chr4_4:69983465-69983484 Msa0699340:three_prime_UTR 25.0%
!! TTTTTATGGATTGCGTTTCT+TGG + chr4_4:69980387-69980406 Msa0699340:intron 30.0%
AAAGGAATGATCAAAAAGAG+AGG - chr4_4:69980469-69980488 None:intergenic 30.0%
CCCACTATGTTAAATACAAA+AGG - chr4_4:69980487-69980506 None:intergenic 30.0%
!!! TGTGTTTTTTTGAGTAATCC+TGG + chr4_4:69980855-69980874 Msa0699340:intron 30.0%
TAGTTAACAAAACAACTTCC+AGG - chr4_4:69980876-69980895 None:intergenic 30.0%
!!! ACATGCTGCTTTTATTTTTG+TGG + chr4_4:69980962-69980981 Msa0699340:intron 30.0%
GTTCTCTTCTTTATTGAAAC+AGG + chr4_4:69981116-69981135 Msa0699340:intron 30.0%
AGTCAAAAGATAGGACAAAA+TGG - chr4_4:69981318-69981337 None:intergenic 30.0%
ATACAGACAAGTCAAAAGAT+AGG - chr4_4:69981327-69981346 None:intergenic 30.0%
!!! TATCTTTTGACTTGTCTGTA+TGG + chr4_4:69981326-69981345 Msa0699340:intron 30.0%
TGTTTACTACCATTATAAGG+CGG - chr4_4:69981484-69981503 None:intergenic 30.0%
GCTTGTTTACTACCATTATA+AGG - chr4_4:69981487-69981506 None:intergenic 30.0%
! CCTTGCATTTTCTTTCAAAA+GGG + chr4_4:69981511-69981530 Msa0699340:CDS 30.0%
! CTTGCATTTTCTTTCAAAAG+GGG + chr4_4:69981512-69981531 Msa0699340:CDS 30.0%
CCAAAAGGTTTCTTAAAACT+TGG - chr4_4:69981649-69981668 None:intergenic 30.0%
!!! CCAAGTTTTAAGAAACCTTT+TGG + chr4_4:69981646-69981665 Msa0699340:CDS 30.0%
!!! AGTTTTAAGAAACCTTTTGG+CGG + chr4_4:69981649-69981668 Msa0699340:CDS 30.0%
TTGAAAAACATGCACAAGAA+AGG + chr4_4:69981674-69981693 Msa0699340:CDS 30.0%
GATGAATTTGGTAAAACAAG+AGG + chr4_4:69982030-69982049 Msa0699340:CDS 30.0%
TCTTTGAATGAATACGATGT+AGG + chr4_4:69982075-69982094 Msa0699340:CDS 30.0%
CTTTGAATGAATACGATGTA+GGG + chr4_4:69982076-69982095 Msa0699340:CDS 30.0%
ATCATATGAGTCATAACCTT+GGG - chr4_4:69982157-69982176 None:intergenic 30.0%
ATGAGTCCTTCTTTCTATTT+GGG - chr4_4:69982223-69982242 None:intergenic 30.0%
AGAATAAATCTTCCACCAAA+TGG + chr4_4:69982429-69982448 Msa0699340:CDS 30.0%
! TTAAGTTCAGCAAGACTTTT+TGG - chr4_4:69982675-69982694 None:intergenic 30.0%
GTACATCATCTTCATATTGT+TGG - chr4_4:69982764-69982783 None:intergenic 30.0%
! AGAATTTCACTAAGTGGTTT+TGG - chr4_4:69982798-69982817 None:intergenic 30.0%
CATGATGACAATAAGAATGA+AGG + chr4_4:69982870-69982889 Msa0699340:CDS 30.0%
AATGAAGGAATTACCGAAAA+CGG + chr4_4:69982885-69982904 Msa0699340:CDS 30.0%
! TATGAGTATGAACAAGTTGA+GGG + chr4_4:69983083-69983102 Msa0699340:CDS 30.0%
GAATATATGGAAGATGAAGA+TGG + chr4_4:69983122-69983141 Msa0699340:CDS 30.0%
ATATATGGAAGATGAAGATG+GGG + chr4_4:69983124-69983143 Msa0699340:CDS 30.0%
!!! TTTTTTCTGCTTAAGCATGT+AGG + chr4_4:69983267-69983286 Msa0699340:three_prime_UTR 30.0%
TATTAACTCTCTAACGTTAC+AGG - chr4_4:69983422-69983441 None:intergenic 30.0%
TAAAACAAGTAAGCGTGGTT+TGG - chr4_4:69979802-69979821 None:intergenic 35.0%
TAAGGTAAAACAAGTAAGCG+TGG - chr4_4:69979807-69979826 None:intergenic 35.0%
GAGTAAGAGAATGTGATGAT+GGG - chr4_4:69979833-69979852 None:intergenic 35.0%
AGAGTAAGAGAATGTGATGA+TGG - chr4_4:69979834-69979853 None:intergenic 35.0%
AGCAAGAAAGTAGACACAAT+CGG - chr4_4:69980100-69980119 None:intergenic 35.0%
! AGAACCTTTTTGCATGTCAA+GGG - chr4_4:69980126-69980145 None:intergenic 35.0%
! AAGAACCTTTTTGCATGTCA+AGG - chr4_4:69980127-69980146 None:intergenic 35.0%
!! GTATTTAACATAGTGGGTGA+AGG + chr4_4:69980490-69980509 Msa0699340:intron 35.0%
GTGAAGGAAATTAGGATGAA+CGG + chr4_4:69980506-69980525 Msa0699340:intron 35.0%
TGAAGGAAATTAGGATGAAC+GGG + chr4_4:69980507-69980526 Msa0699340:intron 35.0%
GAACCCTAATTGTGATTGTT+AGG + chr4_4:69980536-69980555 Msa0699340:intron 35.0%
GGTCCTAACAATCACAATTA+GGG - chr4_4:69980542-69980561 None:intergenic 35.0%
TGGTCCTAACAATCACAATT+AGG - chr4_4:69980543-69980562 None:intergenic 35.0%
CATGTGTATAGTGTAGTGAA+TGG + chr4_4:69980656-69980675 Msa0699340:intron 35.0%
AATAATAATAGGGCCCTGTT+TGG - chr4_4:69980763-69980782 None:intergenic 35.0%
ACTGCTAACAAACACTATAC+AGG - chr4_4:69980835-69980854 None:intergenic 35.0%
ATCCTAGAGATTGTTGGTAT+TGG + chr4_4:69981181-69981200 Msa0699340:CDS 35.0%
AACCAATACCAACAATCTCT+AGG - chr4_4:69981186-69981205 None:intergenic 35.0%
!! GATTGTTGGTATTGGTTGAA+TGG + chr4_4:69981189-69981208 Msa0699340:CDS 35.0%
TTGAAAGAAAATGCAAGGCA+CGG - chr4_4:69981509-69981528 None:intergenic 35.0%
! GCCTTGCATTTTCTTTCAAA+AGG + chr4_4:69981510-69981529 Msa0699340:CDS 35.0%
!! CCCTTTTGAAAGAAAATGCA+AGG - chr4_4:69981514-69981533 None:intergenic 35.0%
! CAAAAGGGGTTTTGCTTAAA+AGG + chr4_4:69981526-69981545 Msa0699340:CDS 35.0%
! CTATTTTACTGCCATTGGTA+GGG - chr4_4:69981582-69981601 None:intergenic 35.0%
!! TGTAGCTATTTTACTGCCAT+TGG - chr4_4:69981587-69981606 None:intergenic 35.0%
CTATGGAAATTGTGATCATC+TGG - chr4_4:69981913-69981932 None:intergenic 35.0%
GATGATCACAATTTCCATAG+TGG + chr4_4:69981913-69981932 Msa0699340:CDS 35.0%
TCACAATTTCCATAGTGGTA+AGG + chr4_4:69981918-69981937 Msa0699340:CDS 35.0%
TATCATGGGGAAGATGAATT+TGG + chr4_4:69982018-69982037 Msa0699340:CDS 35.0%
CGTATTCATTCAAAGAGTCT+GGG - chr4_4:69982071-69982090 None:intergenic 35.0%
TCGTATTCATTCAAAGAGTC+TGG - chr4_4:69982072-69982091 None:intergenic 35.0%
TCATATGAGTCATAACCTTG+GGG - chr4_4:69982156-69982175 None:intergenic 35.0%
GATCATATGAGTCATAACCT+TGG - chr4_4:69982158-69982177 None:intergenic 35.0%
GATGAGTCCTTCTTTCTATT+TGG - chr4_4:69982224-69982243 None:intergenic 35.0%
!!! AGCAAAGTCATCGATTTTCT+CGG + chr4_4:69982349-69982368 Msa0699340:CDS 35.0%
ATCTCTCATTTGAAGGTAAC+TGG - chr4_4:69982386-69982405 None:intergenic 35.0%
TTGACAGATCTCTCATTTGA+AGG - chr4_4:69982393-69982412 None:intergenic 35.0%
ATAAATCTTCCACCAAATGG+TGG + chr4_4:69982432-69982451 Msa0699340:CDS 35.0%
AGTCTTGCTGAACTTAAGTA+TGG + chr4_4:69982678-69982697 Msa0699340:CDS 35.0%
GTCTTGCTGAACTTAAGTAT+GGG + chr4_4:69982679-69982698 Msa0699340:CDS 35.0%
ACCAACAATCATTTGGAAAG+AGG + chr4_4:69982721-69982740 Msa0699340:CDS 35.0%
TCCTCTTTCCAAATGATTGT+TGG - chr4_4:69982725-69982744 None:intergenic 35.0%
GAAGATGATGTACCATTTGA+AGG + chr4_4:69982771-69982790 Msa0699340:CDS 35.0%
ACCACTTAGTGAAATTCTGA+AGG + chr4_4:69982800-69982819 Msa0699340:CDS 35.0%
TCCTTCAGAATTTCACTAAG+TGG - chr4_4:69982804-69982823 None:intergenic 35.0%
! GAAATTCTGAAGGAGAAGAA+AGG + chr4_4:69982810-69982829 Msa0699340:CDS 35.0%
GTATGAGTATGAACAAGTTG+AGG + chr4_4:69983082-69983101 Msa0699340:CDS 35.0%
AAGAACAACACCAATCTTCT+TGG - chr4_4:69983159-69983178 None:intergenic 35.0%
GCTTCCATCATATCAACTAA+AGG - chr4_4:69983472-69983491 None:intergenic 35.0%
!! GAATGTGATGATGGGCTTTA+AGG - chr4_4:69979825-69979844 None:intergenic 40.0%
CCAAATCTCAATCTGAGAAG+AGG - chr4_4:69979958-69979977 None:intergenic 40.0%
CCTCTTCTCAGATTGAGATT+TGG + chr4_4:69979955-69979974 Msa0699340:five_prime_UTR 40.0%
ATCCATCGATCTCTTCTTCT+GGG + chr4_4:69979978-69979997 Msa0699340:five_prime_UTR 40.0%
! AATCTGAAGTTGTGGCTGTT+GGG - chr4_4:69980025-69980044 None:intergenic 40.0%
! TGAGTCTGAATCTGAAGTTG+TGG - chr4_4:69980033-69980052 None:intergenic 40.0%
!! CTTTTCAAGGCTTCATCTTG+AGG - chr4_4:69980072-69980091 None:intergenic 40.0%
CTCAAGATGAAGCCTTGAAA+AGG + chr4_4:69980070-69980089 Msa0699340:CDS 40.0%
!! ACAATCGGTGTTCCTTTTCA+AGG - chr4_4:69980085-69980104 None:intergenic 40.0%
TTCTCCCTTGACATGCAAAA+AGG + chr4_4:69980119-69980138 Msa0699340:CDS 40.0%
!! CATAGTGGGTGAAGGAAATT+AGG + chr4_4:69980498-69980517 Msa0699340:intron 40.0%
GAAGGAAATTAGGATGAACG+GGG + chr4_4:69980508-69980527 Msa0699340:intron 40.0%
! GACATCAGCTTACACTTTTG+TGG - chr4_4:69980611-69980630 None:intergenic 40.0%
GCTGATGTCAATTGAGCATT+TGG + chr4_4:69980622-69980641 Msa0699340:intron 40.0%
TGACTCTAGCATACTCACTA+TGG - chr4_4:69981161-69981180 None:intergenic 40.0%
GAGTCAATCCTAGAGATTGT+TGG + chr4_4:69981175-69981194 Msa0699340:CDS 40.0%
TAAAGAAACTCACCGGATGA+CGG - chr4_4:69981242-69981261 None:intergenic 40.0%
! TTTTACATGCAGCCTCTTGA+CGG + chr4_4:69981370-69981389 Msa0699340:intron 40.0%
!! GCTATTTTACTGCCATTGGT+AGG - chr4_4:69981583-69981602 None:intergenic 40.0%
TGGCAGTAAAATAGCTACAC+AGG + chr4_4:69981588-69981607 Msa0699340:CDS 40.0%
GGCAGTAAAATAGCTACACA+GGG + chr4_4:69981589-69981608 Msa0699340:CDS 40.0%
GCAGTAAAATAGCTACACAG+GGG + chr4_4:69981590-69981609 Msa0699340:CDS 40.0%
TCAATGACAATGACCAACCA+AGG + chr4_4:69981613-69981632 Msa0699340:CDS 40.0%
!! TGTTTTTCAATGCCGCCAAA+AGG - chr4_4:69981664-69981683 None:intergenic 40.0%
AACACCTGCAACATTTGCTT+GGG - chr4_4:69981707-69981726 None:intergenic 40.0%
!! GCAGTCTCAAATTTTTGGAC+AGG - chr4_4:69981739-69981758 None:intergenic 40.0%
! GTGGAGCAGTCTCAAATTTT+TGG - chr4_4:69981744-69981763 None:intergenic 40.0%
ACAAGAGGTCAAGATGAAAG+AGG + chr4_4:69982045-69982064 Msa0699340:CDS 40.0%
GATCAGCCCAAATAGAAAGA+AGG + chr4_4:69982214-69982233 Msa0699340:CDS 40.0%
!!! AAGTCATCGATTTTCTCGGA+AGG + chr4_4:69982353-69982372 Msa0699340:CDS 40.0%
TCTGTCAATAATCGTTTCCG+AGG + chr4_4:69982405-69982424 Msa0699340:CDS 40.0%
GTGGAAGATTTATTCTGCCT+CGG - chr4_4:69982425-69982444 None:intergenic 40.0%
AGGATCCAGGATAGATTAAG+AGG + chr4_4:69982579-69982598 Msa0699340:CDS 40.0%
TCCAGGATAGATTAAGAGGA+AGG + chr4_4:69982583-69982602 Msa0699340:CDS 40.0%
ACCTTCCTCTTAATCTATCC+TGG - chr4_4:69982587-69982606 None:intergenic 40.0%
TTGCTGAACTTAAGTATGGG+AGG + chr4_4:69982682-69982701 Msa0699340:CDS 40.0%
TTGGAAAGAGGAAAAGCTTC+AGG + chr4_4:69982733-69982752 Msa0699340:CDS 40.0%
TGGAAAGAGGAAAAGCTTCA+GGG + chr4_4:69982734-69982753 Msa0699340:CDS 40.0%
!!! AGTGGTTTTGGACCTTCAAA+TGG - chr4_4:69982786-69982805 None:intergenic 40.0%
! GTCATCATGTTTGCTACTGT+GGG - chr4_4:69982859-69982878 None:intergenic 40.0%
! TGTCATCATGTTTGCTACTG+TGG - chr4_4:69982860-69982879 None:intergenic 40.0%
! CTCTGACAATGTTCCGTTTT+CGG - chr4_4:69982901-69982920 None:intergenic 40.0%
GACAATACTGATGCAACTCA+CGG + chr4_4:69982963-69982982 Msa0699340:CDS 40.0%
ACCTTCATACTCCTGATCTT+CGG - chr4_4:69983027-69983046 None:intergenic 40.0%
GGAGACTATGAATATGAGCA+AGG + chr4_4:69983050-69983069 Msa0699340:CDS 40.0%
TGAATATGAGCAAGGTGATG+AGG + chr4_4:69983058-69983077 Msa0699340:CDS 40.0%
GATGACTTTGCCAAGAAGAT+TGG + chr4_4:69983146-69983165 Msa0699340:CDS 40.0%
CTCTCTCTACCCAATTTCTG+TGG + chr4_4:69979904-69979923 Msa0699340:five_prime_UTR 45.0%
TCTCTACCCAATTTCTGTGG+CGG + chr4_4:69979907-69979926 Msa0699340:five_prime_UTR 45.0%
GAGAAGAGGAGAAGAAGTAG+AGG - chr4_4:69979944-69979963 None:intergenic 45.0%
GATCCATCGATCTCTTCTTC+TGG + chr4_4:69979977-69979996 Msa0699340:five_prime_UTR 45.0%
AACCCAGAAGAAGAGATCGA+TGG - chr4_4:69979983-69980002 None:intergenic 45.0%
ATCGATCTCTTCTTCTGGGT+TGG + chr4_4:69979982-69980001 Msa0699340:five_prime_UTR 45.0%
TCGATCTCTTCTTCTGGGTT+GGG + chr4_4:69979983-69980002 Msa0699340:five_prime_UTR 45.0%
! GAATCTGAAGTTGTGGCTGT+TGG - chr4_4:69980026-69980045 None:intergenic 45.0%
! TCATCTTGAGGAGAAGGTGT+TGG - chr4_4:69980060-69980079 None:intergenic 45.0%
AAGGCTTCATCTTGAGGAGA+AGG - chr4_4:69980066-69980085 None:intergenic 45.0%
CTCTAGCATACTCACTATGG+CGG - chr4_4:69981158-69981177 None:intergenic 45.0%
!! GGATGACGGAATGAACACTT+GGG - chr4_4:69981228-69981247 None:intergenic 45.0%
CAAGTGTTCATTCCGTCATC+CGG + chr4_4:69981227-69981246 Msa0699340:CDS 45.0%
! GTGCTGGTAAAGAAACTCAC+CGG - chr4_4:69981249-69981268 None:intergenic 45.0%
AAAGGACAGAAAACTCGTGC+TGG - chr4_4:69981265-69981284 None:intergenic 45.0%
TTTACATGCAGCCTCTTGAC+GGG + chr4_4:69981371-69981390 Msa0699340:intron 45.0%
ACCATTATAAGGCGGTGCAT+GGG - chr4_4:69981476-69981495 None:intergenic 45.0%
TACCATTATAAGGCGGTGCA+TGG - chr4_4:69981477-69981496 None:intergenic 45.0%
TTACTGCCATTGGTAGGGTT+TGG - chr4_4:69981577-69981596 None:intergenic 45.0%
AACTTGGATTCTCAGCTCCT+TGG - chr4_4:69981633-69981652 None:intergenic 45.0%
ACTTCCCAAGCAAATGTTGC+AGG + chr4_4:69981700-69981719 Msa0699340:CDS 45.0%
CAACACCTGCAACATTTGCT+TGG - chr4_4:69981708-69981727 None:intergenic 45.0%
TCATTATCTAACCCTGCAGG+TGG - chr4_4:69981805-69981824 None:intergenic 45.0%
ACCTGCAGGGTTAGATAATG+AGG + chr4_4:69981804-69981823 Msa0699340:CDS 45.0%
TTGATGCACACGATTAGACC+GGG - chr4_4:69981881-69981900 None:intergenic 45.0%
GTTGATGCACACGATTAGAC+CGG - chr4_4:69981882-69981901 None:intergenic 45.0%
TTGTGATCATCTGGCAAACG+AGG - chr4_4:69981904-69981923 None:intergenic 45.0%
CGTCATTGTCCTTACCACTA+TGG - chr4_4:69981930-69981949 None:intergenic 45.0%
GCTACAAGAACATCAAACCC+AGG - chr4_4:69981973-69981992 None:intergenic 45.0%
GATCACATGCAAGAGCCATA+TGG + chr4_4:69982174-69982193 Msa0699340:CDS 45.0%
TTTCTAGGTTCCCAGCCATA+TGG - chr4_4:69982192-69982211 None:intergenic 45.0%
ATATGGCTGGGAACCTAGAA+AGG + chr4_4:69982191-69982210 Msa0699340:CDS 45.0%
TATGGCTGGGAACCTAGAAA+GGG + chr4_4:69982192-69982211 Msa0699340:CDS 45.0%
ATTTGGGCTGATCCCTTTCT+AGG - chr4_4:69982207-69982226 None:intergenic 45.0%
TGCGCTTGGATAAACGATGT+CGG - chr4_4:69982287-69982306 None:intergenic 45.0%
TTGGACAGAGGAAGAATCAG+TGG + chr4_4:69982516-69982535 Msa0699340:CDS 45.0%
AAGTTTCTCCTCTCGTCATC+TGG - chr4_4:69982612-69982631 None:intergenic 45.0%
CTGAAGGAGAAGAAAGGAGT+TGG + chr4_4:69982816-69982835 Msa0699340:CDS 45.0%
! ATCATGTTTGCTACTGTGGG+AGG - chr4_4:69982856-69982875 None:intergenic 45.0%
GGAAGAAGAGTCCAAGATTC+AGG + chr4_4:69982926-69982945 Msa0699340:CDS 45.0%
AACTCACGGTCAATCATCTG+AGG + chr4_4:69982977-69982996 Msa0699340:CDS 45.0%
! GATCAGGAGTATGAAGGTGA+AGG + chr4_4:69983029-69983048 Msa0699340:CDS 45.0%
TTAAGCATGTAGGCCTCAAC+TGG + chr4_4:69983277-69983296 Msa0699340:three_prime_UTR 45.0%
TAAGCATGTAGGCCTCAACT+GGG + chr4_4:69983278-69983297 Msa0699340:three_prime_UTR 45.0%
GACAGATATTACCCCAGTTG+AGG - chr4_4:69983293-69983312 None:intergenic 45.0%
!!! AAAAATTTAATTTTGATTAA+TGG + chr4_4:69980265-69980284 Msa0699340:intron 5.0%
!!! AAAATTTAATTTTGATTAAT+GGG + chr4_4:69980266-69980285 Msa0699340:intron 5.0%
!! TAATAAAATAACAAAAATTA+TGG - chr4_4:69980563-69980582 None:intergenic 5.0%
!!! CTTTTTATTTTAATAATAAT+AGG - chr4_4:69980774-69980793 None:intergenic 5.0%
AGAGAGCCGCCACAGAAATT+GGG - chr4_4:69979916-69979935 None:intergenic 50.0%
TTGTGGCTGTGCGAATCATG+TGG + chr4_4:69980979-69980998 Msa0699340:intron 50.0%
!! CGGATGACGGAATGAACACT+TGG - chr4_4:69981229-69981248 None:intergenic 50.0%
GTTCCTAACAACCCGTCAAG+AGG - chr4_4:69981385-69981404 None:intergenic 50.0%
ACCCATGCACCGCCTTATAA+TGG + chr4_4:69981472-69981491 Msa0699340:CDS 50.0%
CATGGACCAAACCCTACCAA+TGG + chr4_4:69981568-69981587 Msa0699340:CDS 50.0%
TGGATTCTCAGCTCCTTGGT+TGG - chr4_4:69981629-69981648 None:intergenic 50.0%
TTATCTAACCCTGCAGGTGG+TGG - chr4_4:69981802-69981821 None:intergenic 50.0%
GCCTCATTATCTAACCCTGC+AGG - chr4_4:69981808-69981827 None:intergenic 50.0%
ACACGATTAGACCGGGCATT+AGG - chr4_4:69981874-69981893 None:intergenic 50.0%
TTTCTCAGAGAGTCGTCTCC+TGG + chr4_4:69981952-69981971 Msa0699340:CDS 50.0%
TTCTCAGAGAGTCGTCTCCT+GGG + chr4_4:69981953-69981972 Msa0699340:CDS 50.0%
GATAGATTTCGTGTGCCCCA+AGG + chr4_4:69982138-69982157 Msa0699340:CDS 50.0%
ACATGCAAGAGCCATATGGC+TGG + chr4_4:69982178-69982197 Msa0699340:CDS 50.0%
CATGCAAGAGCCATATGGCT+GGG + chr4_4:69982179-69982198 Msa0699340:CDS 50.0%
TCGTAGTCCTGACAGTGTTG+AGG + chr4_4:69982251-69982270 Msa0699340:CDS 50.0%
! TCTGAGACCTCAACACTGTC+AGG - chr4_4:69982261-69982280 None:intergenic 50.0%
GACATCGTTTATCCAAGCGC+AGG + chr4_4:69982286-69982305 Msa0699340:CDS 50.0%
TCGTTTATCCAAGCGCAGGA+AGG + chr4_4:69982290-69982309 Msa0699340:CDS 50.0%
CGTTTATCCAAGCGCAGGAA+GGG + chr4_4:69982291-69982310 Msa0699340:CDS 50.0%
ATCTGTCGTCGCTCATGATG+AGG + chr4_4:69982326-69982345 Msa0699340:CDS 50.0%
TGACTGTCACCACCATTTGG+TGG - chr4_4:69982444-69982463 None:intergenic 50.0%
AGGTGACTGTCACCACCATT+TGG - chr4_4:69982447-69982466 None:intergenic 50.0%
TGGTGACAGTCACCTAGAGA+GGG + chr4_4:69982452-69982471 Msa0699340:CDS 50.0%
CTCTGTCTAAGTCCCTCTCT+AGG - chr4_4:69982467-69982486 None:intergenic 50.0%
CTAGAGAGGGACTTAGACAG+AGG + chr4_4:69982465-69982484 Msa0699340:CDS 50.0%
AACCAGCAGTCACCTAGAGA+GGG + chr4_4:69982491-69982510 Msa0699340:CDS 50.0%
ACAGAGGAAGAATCAGTGGC+CGG + chr4_4:69982520-69982539 Msa0699340:CDS 50.0%
GAAGCCTTCCAGATGACAAC+CGG - chr4_4:69982542-69982561 None:intergenic 50.0%
AAGGCTTCAATCGCCTCATG+AGG + chr4_4:69982554-69982573 Msa0699340:CDS 50.0%
AGGCTTCAATCGCCTCATGA+GGG + chr4_4:69982555-69982574 Msa0699340:CDS 50.0%
TTCAATCGCCTCATGAGGGA+AGG + chr4_4:69982559-69982578 Msa0699340:CDS 50.0%
CTTCAGCAGTCGATCGAATG+AGG + chr4_4:69982629-69982648 Msa0699340:CDS 50.0%
GATCGAATGAGGACCGAAGT+GGG + chr4_4:69982640-69982659 Msa0699340:CDS 50.0%
GAGAAGAAAGGAGTTGGAGC+TGG + chr4_4:69982822-69982841 Msa0699340:CDS 50.0%
CGGAACATTGTCAGAGAGCA+AGG + chr4_4:69982905-69982924 Msa0699340:CDS 50.0%
TCCAAGATTCAGGCTGCTGA+TGG + chr4_4:69982936-69982955 Msa0699340:CDS 50.0%
TCCATCAGCAGCCTGAATCT+TGG - chr4_4:69982940-69982959 None:intergenic 50.0%
CACGGTCAATCATCTGAGGA+AGG + chr4_4:69982981-69983000 Msa0699340:CDS 50.0%
! GCCGAAGATCAGGAGTATGA+AGG + chr4_4:69983023-69983042 Msa0699340:CDS 50.0%
AAGCATGTAGGCCTCAACTG+GGG + chr4_4:69983279-69983298 Msa0699340:three_prime_UTR 50.0%
GAGAGAGCCGCCACAGAAAT+TGG - chr4_4:69979917-69979936 None:intergenic 55.0%
TTGTGGCTGTTGGGCAACCA+TGG - chr4_4:69980016-69980035 None:intergenic 55.0%
!! CAGCCTCTTGACGGGTTGTT+AGG + chr4_4:69981379-69981398 Msa0699340:intron 55.0%
CGGGTTGTTAGGAACACCAC+CGG + chr4_4:69981390-69981409 Msa0699340:CDS 55.0%
GCAGATGTTGCCGCAATCTG+TGG - chr4_4:69981451-69981470 None:intergenic 55.0%
GACAGATGTGCCTTCCTGCA+TGG + chr4_4:69981550-69981569 Msa0699340:CDS 55.0%
! AGGGTTTGGTCCATGCAGGA+AGG - chr4_4:69981563-69981582 None:intergenic 55.0%
! TGGTAGGGTTTGGTCCATGC+AGG - chr4_4:69981567-69981586 None:intergenic 55.0%
AGTTCTCCACCGCCAACCAA+TGG + chr4_4:69981847-69981866 Msa0699340:CDS 55.0%
GTTCTCCACCGCCAACCAAT+GGG + chr4_4:69981848-69981867 Msa0699340:CDS 55.0%
! GCATTAGGCCCATTGGTTGG+CGG - chr4_4:69981859-69981878 None:intergenic 55.0%
CAACCAATGGGCCTAATGCC+CGG + chr4_4:69981860-69981879 Msa0699340:CDS 55.0%
TCCAAGCGCAGGAAGGGAAA+CGG + chr4_4:69982297-69982316 Msa0699340:CDS 55.0%
GTGGTGACAGTCACCTAGAG+AGG + chr4_4:69982451-69982470 Msa0699340:CDS 55.0%
GAACCAGCAGTCACCTAGAG+AGG + chr4_4:69982490-69982509 Msa0699340:CDS 55.0%
CAGTCACCTAGAGAGGGACT+TGG + chr4_4:69982458-69982477 Msa0699340:CDS 55.0%
CTCTGTCCAAGTCCCTCTCT+AGG - chr4_4:69982506-69982525 None:intergenic 55.0%
CTAGAGAGGGACTTGGACAG+AGG + chr4_4:69982504-69982523 Msa0699340:CDS 55.0%
ATCAGTGGCCGGTTGTCATC+TGG + chr4_4:69982531-69982550 Msa0699340:CDS 55.0%
TCCTGGATCCTTCCCTCATG+AGG - chr4_4:69982570-69982589 None:intergenic 55.0%
GAAGGTTGCCAGATGACGAG+AGG + chr4_4:69982601-69982620 Msa0699340:CDS 55.0%
CGATCGAATGAGGACCGAAG+TGG + chr4_4:69982639-69982658 Msa0699340:CDS 55.0%
TTGGCGCAGAGAACCCACTT+CGG - chr4_4:69982656-69982675 None:intergenic 55.0%
AAAGGAGTTGGAGCTGGTGC+AGG + chr4_4:69982828-69982847 Msa0699340:CDS 55.0%
CGATGAAGCTGCCGAAGATC+AGG + chr4_4:69983013-69983032 Msa0699340:CDS 55.0%
! CTGGGTTGGGTTGTTCGCCA+TGG + chr4_4:69979996-69980015 Msa0699340:exon 60.0%
ATCTGTGGCGTGGGGACAGA+TGG - chr4_4:69981436-69981455 None:intergenic 60.0%
TGTCCCCACGCCACAGATTG+CGG + chr4_4:69981438-69981457 Msa0699340:CDS 60.0%
TTGCCGCAATCTGTGGCGTG+GGG - chr4_4:69981444-69981463 None:intergenic 60.0%
GTTGCCGCAATCTGTGGCGT+GGG - chr4_4:69981445-69981464 None:intergenic 60.0%
TGTTGCCGCAATCTGTGGCG+TGG - chr4_4:69981446-69981465 None:intergenic 60.0%
ATGTACCGCCACCACCTGCA+GGG + chr4_4:69981791-69981810 Msa0699340:CDS 60.0%
! TCTAACCCTGCAGGTGGTGG+CGG - chr4_4:69981799-69981818 None:intergenic 60.0%
! TTAGGCCCATTGGTTGGCGG+TGG - chr4_4:69981856-69981875 None:intergenic 60.0%
CGGGCATTAGGCCCATTGGT+TGG - chr4_4:69981862-69981881 None:intergenic 60.0%
AGACCGGGCATTAGGCCCAT+TGG - chr4_4:69981866-69981885 None:intergenic 60.0%
GTCCCTCTCTAGGTGACTGC+TGG - chr4_4:69982496-69982515 None:intergenic 60.0%
GTGGCCGGTTGTCATCTGGA+AGG + chr4_4:69982535-69982554 Msa0699340:CDS 60.0%
GCCTCATGAGGGAAGGATCC+AGG + chr4_4:69982566-69982585 Msa0699340:CDS 60.0%
CCAGCAGCAGCAGCAGTTGC+CGG - chr4_4:69981412-69981431 None:intergenic 65.0%
CATGTACCGCCACCACCTGC+AGG + chr4_4:69981790-69981809 Msa0699340:CDS 65.0%
GCCGTTTCCCTTCCTGCGCT+TGG - chr4_4:69982301-69982320 None:intergenic 65.0%
GCAGCAGCAGCAGTTGCCGG+TGG - chr4_4:69981409-69981428 None:intergenic 70.0%
CCGGCAACTGCTGCTGCTGC+TGG + chr4_4:69981409-69981428 Msa0699340:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 69979732 69983510 69979732 ID=Msa0699340;Name=Msa0699340
chr4_4 mRNA 69979732 69983510 69979732 ID=Msa0699340-mRNA-1;Parent=Msa0699340;Name=Msa0699340-mRNA-1;_AED=0.00;_eAED=0.00;_QI=283|1|1|1|1|1|3|326|679
chr4_4 exon 69979732 69980140 69979732 ID=Msa0699340-mRNA-1:exon:21038;Parent=Msa0699340-mRNA-1
chr4_4 exon 69981138 69981248 69981138 ID=Msa0699340-mRNA-1:exon:21039;Parent=Msa0699340-mRNA-1
chr4_4 exon 69981382 69983510 69981382 ID=Msa0699340-mRNA-1:exon:21040;Parent=Msa0699340-mRNA-1
chr4_4 five_prime_UTR 69979732 69980014 69979732 ID=Msa0699340-mRNA-1:five_prime_utr;Parent=Msa0699340-mRNA-1
chr4_4 CDS 69980015 69980140 69980015 ID=Msa0699340-mRNA-1:cds;Parent=Msa0699340-mRNA-1
chr4_4 CDS 69981138 69981248 69981138 ID=Msa0699340-mRNA-1:cds;Parent=Msa0699340-mRNA-1
chr4_4 CDS 69981382 69983184 69981382 ID=Msa0699340-mRNA-1:cds;Parent=Msa0699340-mRNA-1
chr4_4 three_prime_UTR 69983185 69983510 69983185 ID=Msa0699340-mRNA-1:three_prime_utr;Parent=Msa0699340-mRNA-1
Gene Sequence

>Msa0699340

ATGGTTGCCCAACAGCCACAACTTCAGATTCAGACTCAGCAACCAACACCTTCTCCTCAAGATGAAGCCTTGAAAAGGAACACCGATTGTGTCTACTTTCTTGCTTCTCCCTTGACATGCAAAAAGGGAAACGAATGTGAGTACCGCCATAGTGAGTATGCTAGAGTCAATCCTAGAGATTGTTGGTATTGGTTGAATGGTAACTGCTTGAATCCCAAGTGTTCATTCCGTCATCCGCCTCTTGACGGGTTGTTAGGAACACCACCGGCAACTGCTGCTGCTGCTGGACCATCTGTCCCCACGCCACAGATTGCGGCAACATCTGCAACCCATGCACCGCCTTATAATGGTAGTAAACAAGCCGTGCCTTGCATTTTCTTTCAAAAGGGGTTTTGCTTAAAAGGTGACAGATGTGCCTTCCTGCATGGACCAAACCCTACCAATGGCAGTAAAATAGCTACACAGGGGTCAATGACAATGACCAACCAAGGAGCTGAGAATCCAAGTTTTAAGAAACCTTTTGGCGGCATTGAAAAACATGCACAAGAAAGGAAAACTTCCCAAGCAAATGTTGCAGGTGTTGAAGCTAAACCTGTCCAAAAATTTGAGACTGCTCCACAGAAAAATATGTTTAAGTTGGTGAAGCATGTACCGCCACCACCTGCAGGGTTAGATAATGAGGCTTCGCGATTTAAGACAACTAGTTCTCCACCGCCAACCAATGGGCCTAATGCCCGGTCTAATCGTGTGCATCAACCTCGTTTGCCAGATGATCACAATTTCCATAGTGGTAAGGACAATGACGAGTTTCTCAGAGAGTCGTCTCCTGGGTTTGATGTTCTTGTAGCTGACGAACTTAGAAATTCTGATTACTATCATGGGGAAGATGAATTTGGTAAAACAAGAGGTCAAGATGAAAGAGGCCCAGACTCTTTGAATGAATACGATGTAGGGCATTCTGCTGATTATAATTTAGCTGCTGATATTGATCGAGATAGATTTCGTGTGCCCCAAGGTTATGACTCATATGATCACATGCAAGAGCCATATGGCTGGGAACCTAGAAAGGGATCAGCCCAAATAGAAAGAAGGACTCATCACAGATCTCGTAGTCCTGACAGTGTTGAGGTCTCAGATCTCCGACATCGTTTATCCAAGCGCAGGAAGGGAAACGGCTTAAAATCTGTCGTCGCTCATGATGAGGAGCAAAGTCATCGATTTTCTCGGAAGGATTCACACCAGTTACCTTCAAATGAGAGATCTGTCAATAATCGTTTCCGAGGCAGAATAAATCTTCCACCAAATGGTGGTGACAGTCACCTAGAGAGGGACTTAGACAGAGGAAGAACCAGCAGTCACCTAGAGAGGGACTTGGACAGAGGAAGAATCAGTGGCCGGTTGTCATCTGGAAGGCTTCAATCGCCTCATGAGGGAAGGATCCAGGATAGATTAAGAGGAAGGTTGCCAGATGACGAGAGGAGAAACTTCAGCAGTCGATCGAATGAGGACCGAAGTGGGTTCTCTGCGCCAAAAAGTCTTGCTGAACTTAAGTATGGGAGGAACACTGAGAATAATGACCAACAATCATTTGGAAAGAGGAAAAGCTTCAGGGATCACCAACAATATGAAGATGATGTACCATTTGAAGGTCCAAAACCACTTAGTGAAATTCTGAAGGAGAAGAAAGGAGTTGGAGCTGGTGCAGGCTCCTCCCACAGTAGCAAACATGATGACAATAAGAATGAAGGAATTACCGAAAACGGAACATTGTCAGAGAGCAAGGAAGAAGAGTCCAAGATTCAGGCTGCTGATGGAGTTGACAATACTGATGCAACTCACGGTCAATCATCTGAGGAAGGAATGATATACGATGAAGCTGCCGAAGATCAGGAGTATGAAGGTGAAGGAGACTATGAATATGAGCAAGGTGATGAGGAGTATGAGTATGAACAAGTTGAGGGTGAAAATCAAGAGCAAGAATATATGGAAGATGAAGATGGGGATGACTTTGCCAAGAAGATTGGTGTTGTTCTTTCATAA
Protein sequence

>Msa0699340

MVAQQPQLQIQTQQPTPSPQDEALKRNTDCVYFLASPLTCKKGNECEYRHSEYARVNPRDCWYWLNGNCLNPKCSFRHPPLDGLLGTPPATAAAAGPSVPTPQIAATSATHAPPYNGSKQAVPCIFFQKGFCLKGDRCAFLHGPNPTNGSKIATQGSMTMTNQGAENPSFKKPFGGIEKHAQERKTSQANVAGVEAKPVQKFETAPQKNMFKLVKHVPPPPAGLDNEASRFKTTSSPPPTNGPNARSNRVHQPRLPDDHNFHSGKDNDEFLRESSPGFDVLVADELRNSDYYHGEDEFGKTRGQDERGPDSLNEYDVGHSADYNLAADIDRDRFRVPQGYDSYDHMQEPYGWEPRKGSAQIERRTHHRSRSPDSVEVSDLRHRLSKRRKGNGLKSVVAHDEEQSHRFSRKDSHQLPSNERSVNNRFRGRINLPPNGGDSHLERDLDRGRTSSHLERDLDRGRISGRLSSGRLQSPHEGRIQDRLRGRLPDDERRNFSSRSNEDRSGFSAPKSLAELKYGRNTENNDQQSFGKRKSFRDHQQYEDDVPFEGPKPLSEILKEKKGVGAGAGSSHSSKHDDNKNEGITENGTLSESKEEESKIQAADGVDNTDATHGQSSEEGMIYDEAAEDQEYEGEGDYEYEQGDEEYEYEQVEGENQEQEYMEDEDGDDFAKKIGVVLS*