Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0702910 | AET04474.1 | 92.308 | 156 | 12 | 0 | 1 | 156 | 1 | 156 | 1.93e-95 | 283 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0702910 | G7L9K0 | 92.308 | 156 | 12 | 0 | 1 | 156 | 1 | 156 | 9.24e-96 | 283 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0702910 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATACCTTCAAATGAAAT+TGG | 0.214941 | 4_4:+74755008 | Msa0702910:CDS |
| CATAAAACTGGCTTGTCTTT+TGG | 0.254968 | 4_4:-74754514 | None:intergenic |
| CTTTGAGGTTTCCCCAGTTT+TGG | 0.256311 | 4_4:-74754558 | None:intergenic |
| ATTCTCAAACTACGTCTCTT+TGG | 0.299791 | 4_4:-74754861 | None:intergenic |
| TCAAACAATAAGGCCTCTTC+TGG | 0.301830 | 4_4:-74754972 | None:intergenic |
| TGGCGCCATGCTGGAGTTGC+TGG | 0.314815 | 4_4:-74754487 | None:intergenic |
| GATGTTTCTGATTCAGATAA+TGG | 0.318368 | 4_4:+74754673 | Msa0702910:CDS |
| CTGTTCGTAACAAGTTCTTT+TGG | 0.340372 | 4_4:-74754604 | None:intergenic |
| TTTGGTCCCTGGCGCCATGC+TGG | 0.374123 | 4_4:-74754496 | None:intergenic |
| GAAAACAACGTTTGAGAGAA+TGG | 0.387976 | 4_4:-74754582 | None:intergenic |
| GTGGACTAAGATACCAAAAC+TGG | 0.405891 | 4_4:+74754545 | Msa0702910:CDS |
| CCACATGCATTGCATAAAAC+TGG | 0.410323 | 4_4:-74754526 | None:intergenic |
| CCTTCAAATGAAATTGGCCT+TGG | 0.419265 | 4_4:+74755014 | Msa0702910:CDS |
| CCAAGGCCAATTTCATTTGA+AGG | 0.421742 | 4_4:-74755014 | None:intergenic |
| AGCAACTCCAGCATGGCGCC+AGG | 0.436034 | 4_4:+74754489 | Msa0702910:CDS |
| GTTTGAGAGAATGGTCTTTG+AGG | 0.475222 | 4_4:-74754573 | None:intergenic |
| TCTTCTGGAGATGACTCATC+TGG | 0.489997 | 4_4:-74754957 | None:intergenic |
| TCTTGTTGAAACTTCTCCAT+CGG | 0.530728 | 4_4:-74754903 | None:intergenic |
| ATCTGAATCAGAAACATCAA+TGG | 0.539843 | 4_4:-74754668 | None:intergenic |
| TTCTACATAGAAGAACCGTC+TGG | 0.563851 | 4_4:-74755053 | None:intergenic |
| GCAACTCCAGCATGGCGCCA+GGG | 0.585142 | 4_4:+74754490 | Msa0702910:CDS |
| AGATGAGTCATCTCCAGAAG+AGG | 0.604525 | 4_4:+74754959 | Msa0702910:CDS |
| GCGGTTCTTCTTAAACCAGA+CGG | 0.605404 | 4_4:+74755038 | Msa0702910:CDS |
| AAATGAAATTGGCCTTGGAG+CGG | 0.608265 | 4_4:+74755019 | Msa0702910:CDS |
| CAATCAGAAGATGACACCGA+TGG | 0.614119 | 4_4:+74754887 | Msa0702910:CDS |
| TGGACTAAGATACCAAAACT+GGG | 0.618651 | 4_4:+74754546 | Msa0702910:CDS |
| GTTTAAGAAGAACCGCTCCA+AGG | 0.637380 | 4_4:-74755031 | None:intergenic |
| AAAGAACTTGTTACGAACAG+TGG | 0.644687 | 4_4:+74754607 | Msa0702910:CDS |
| CATCACCAGCAACTCCAGCA+TGG | 0.651498 | 4_4:+74754482 | Msa0702910:CDS |
| GGACTAAGATACCAAAACTG+GGG | 0.731002 | 4_4:+74754547 | Msa0702910:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGATATATATTACTTTTGTG+AGG | + | chr4_4:74754742-74754761 | Msa0702910:intron | 20.0% |
| !!! | GATATATATTACTTTTGTGA+GGG | + | chr4_4:74754743-74754762 | Msa0702910:intron | 20.0% |
| ! | TGTAAAGAAAAGAGTGAAAA+TGG | - | chr4_4:74754721-74754740 | None:intergenic | 25.0% |
| !! | GTTCACATTTTCAAACAATA+AGG | - | chr4_4:74754985-74755004 | None:intergenic | 25.0% |
| ! | TTCATACCTTCAAATGAAAT+TGG | + | chr4_4:74755008-74755027 | Msa0702910:CDS | 25.0% |
| ATCTGAATCAGAAACATCAA+TGG | - | chr4_4:74754671-74754690 | None:intergenic | 30.0% | |
| ! | GATGTTTCTGATTCAGATAA+TGG | + | chr4_4:74754673-74754692 | Msa0702910:CDS | 30.0% |
| !! | ATATATTACTTTTGTGAGGG+TGG | + | chr4_4:74754746-74754765 | Msa0702910:intron | 30.0% |
| !! | TATATTACTTTTGTGAGGGT+GGG | + | chr4_4:74754747-74754766 | Msa0702910:intron | 30.0% |
| !!! | CATAAAACTGGCTTGTCTTT+TGG | - | chr4_4:74754517-74754536 | None:intergenic | 35.0% |
| TGGACTAAGATACCAAAACT+GGG | + | chr4_4:74754546-74754565 | Msa0702910:CDS | 35.0% | |
| GAAAACAACGTTTGAGAGAA+TGG | - | chr4_4:74754585-74754604 | None:intergenic | 35.0% | |
| ! | CTGTTCGTAACAAGTTCTTT+TGG | - | chr4_4:74754607-74754626 | None:intergenic | 35.0% |
| !! | AAAGAACTTGTTACGAACAG+TGG | + | chr4_4:74754607-74754626 | Msa0702910:CDS | 35.0% |
| ATTCTCAAACTACGTCTCTT+TGG | - | chr4_4:74754864-74754883 | None:intergenic | 35.0% | |
| TCTTGTTGAAACTTCTCCAT+CGG | - | chr4_4:74754906-74754925 | None:intergenic | 35.0% | |
| CCACATGCATTGCATAAAAC+TGG | - | chr4_4:74754529-74754548 | None:intergenic | 40.0% | |
| !! | CCAGTTTTATGCAATGCATG+TGG | + | chr4_4:74754526-74754545 | Msa0702910:CDS | 40.0% |
| ! | GTGGACTAAGATACCAAAAC+TGG | + | chr4_4:74754545-74754564 | Msa0702910:CDS | 40.0% |
| GGACTAAGATACCAAAACTG+GGG | + | chr4_4:74754547-74754566 | Msa0702910:CDS | 40.0% | |
| GTTTGAGAGAATGGTCTTTG+AGG | - | chr4_4:74754576-74754595 | None:intergenic | 40.0% | |
| TCAAACAATAAGGCCTCTTC+TGG | - | chr4_4:74754975-74754994 | None:intergenic | 40.0% | |
| CCAAGGCCAATTTCATTTGA+AGG | - | chr4_4:74755017-74755036 | None:intergenic | 40.0% | |
| ! | CCTTCAAATGAAATTGGCCT+TGG | + | chr4_4:74755014-74755033 | Msa0702910:CDS | 40.0% |
| !! | AAATGAAATTGGCCTTGGAG+CGG | + | chr4_4:74755019-74755038 | Msa0702910:CDS | 40.0% |
| !!! | CTTTGAGGTTTCCCCAGTTT+TGG | - | chr4_4:74754561-74754580 | None:intergenic | 45.0% |
| CAATCAGAAGATGACACCGA+TGG | + | chr4_4:74754887-74754906 | Msa0702910:CDS | 45.0% | |
| ! | TCTTCTGGAGATGACTCATC+TGG | - | chr4_4:74754960-74754979 | None:intergenic | 45.0% |
| AGATGAGTCATCTCCAGAAG+AGG | + | chr4_4:74754959-74754978 | Msa0702910:CDS | 45.0% | |
| GTTTAAGAAGAACCGCTCCA+AGG | - | chr4_4:74755034-74755053 | None:intergenic | 45.0% | |
| GCGGTTCTTCTTAAACCAGA+CGG | + | chr4_4:74755038-74755057 | Msa0702910:CDS | 45.0% | |
| CATCACCAGCAACTCCAGCA+TGG | + | chr4_4:74754482-74754501 | Msa0702910:CDS | 55.0% | |
| !! | CTGGCTTGTCTTTTGGTCCC+TGG | - | chr4_4:74754510-74754529 | None:intergenic | 55.0% |
| TGGCGCCATGCTGGAGTTGC+TGG | - | chr4_4:74754490-74754509 | None:intergenic | 65.0% | |
| AGCAACTCCAGCATGGCGCC+AGG | + | chr4_4:74754489-74754508 | Msa0702910:CDS | 65.0% | |
| GCAACTCCAGCATGGCGCCA+GGG | + | chr4_4:74754490-74754509 | Msa0702910:CDS | 65.0% | |
| TTTGGTCCCTGGCGCCATGC+TGG | - | chr4_4:74754499-74754518 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 74754475 | 74755073 | 74754475 | ID=Msa0702910;Name=Msa0702910 |
| chr4_4 | mRNA | 74754475 | 74755073 | 74754475 | ID=Msa0702910-mRNA-1;Parent=Msa0702910;Name=Msa0702910-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|0|0|1|1|1|2|0|156 |
| chr4_4 | exon | 74754475 | 74754694 | 74754475 | ID=Msa0702910-mRNA-1:exon:23318;Parent=Msa0702910-mRNA-1 |
| chr4_4 | exon | 74754823 | 74755073 | 74754823 | ID=Msa0702910-mRNA-1:exon:23319;Parent=Msa0702910-mRNA-1 |
| chr4_4 | CDS | 74754475 | 74754694 | 74754475 | ID=Msa0702910-mRNA-1:cds;Parent=Msa0702910-mRNA-1 |
| chr4_4 | CDS | 74754823 | 74755073 | 74754823 | ID=Msa0702910-mRNA-1:cds;Parent=Msa0702910-mRNA-1 |
| Gene Sequence |
| Protein sequence |
