Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0702920 | AET04474.1 | 88.816 | 152 | 14 | 1 | 4 | 152 | 5 | 156 | 1.22e-85 | 258 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0702920 | G7L9K0 | 88.816 | 152 | 14 | 1 | 4 | 152 | 5 | 156 | 5.84e-86 | 258 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0702920 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAAAACTGGCTTCTCTTC+TGG | 0.255261 | 4_4:-74765873 | None:intergenic |
| TCGAACAATAAGGCCTCTTC+TGG | 0.271838 | 4_4:-74766322 | None:intergenic |
| TTCATACCTTCAAATGAAAT+TGG | 0.274802 | 4_4:+74766358 | Msa0702920:CDS |
| CTTCAAGATTTCCCCAGTTT+TGG | 0.287698 | 4_4:-74765917 | None:intergenic |
| TGGCGCCATGCTGGAGTTGC+TGG | 0.300530 | 4_4:-74765846 | None:intergenic |
| GATGTTTCTGATTCAGATAA+TGG | 0.318368 | 4_4:+74766032 | Msa0702920:CDS |
| ATCCTCAAACTACGCCTCTT+TGG | 0.326587 | 4_4:-74766220 | None:intergenic |
| CTGTTCGTAACAAGTTCTTT+TGG | 0.340372 | 4_4:-74765963 | None:intergenic |
| TCTGGTCCCTGGCGCCATGC+TGG | 0.378150 | 4_4:-74765855 | None:intergenic |
| CCAAGACCAATTTCATTTGA+AGG | 0.378872 | 4_4:-74766364 | None:intergenic |
| CCTTCAAATGAAATTGGTCT+TGG | 0.383519 | 4_4:+74766364 | Msa0702920:CDS |
| GTGGACTAAGATACCAAAAC+TGG | 0.405891 | 4_4:+74765904 | Msa0702920:CDS |
| CCACATGCATTGCATAAAAC+TGG | 0.410323 | 4_4:-74765885 | None:intergenic |
| AGCAACTCCAGCATGGCGCC+AGG | 0.413779 | 4_4:+74765848 | Msa0702920:CDS |
| CTCCAAAGAGGCGTAGTTTG+AGG | 0.425603 | 4_4:+74766218 | Msa0702920:CDS |
| CGAACAATAAGGCCTCTTCT+GGG | 0.435884 | 4_4:-74766321 | None:intergenic |
| CTGGCTTCTCTTCTGGTCCC+TGG | 0.474139 | 4_4:-74765866 | None:intergenic |
| TCTTCTGGGGATGACTCATC+TGG | 0.478086 | 4_4:-74766307 | None:intergenic |
| GAGGATTATCAATCAGAAGA+TGG | 0.496715 | 4_4:+74766237 | Msa0702920:CDS |
| TTCATATTCATGCAAGTCTA+CGG | 0.538240 | 4_4:+74766192 | Msa0702920:CDS |
| ATCTGAATCAGAAACATCAA+TGG | 0.539843 | 4_4:-74766027 | None:intergenic |
| TTCTACATAGAAGAACCGTC+TGG | 0.563851 | 4_4:-74766403 | None:intergenic |
| GCAACTCCAGCATGGCGCCA+GGG | 0.572723 | 4_4:+74765849 | Msa0702920:CDS |
| TTTGGAAAACAACGTTTGAG+AGG | 0.575726 | 4_4:-74765945 | None:intergenic |
| AGATGAGTCATCCCCAGAAG+AGG | 0.591895 | 4_4:+74766309 | Msa0702920:CDS |
| GCAGTTCTTCTTAAACCAGA+CGG | 0.605128 | 4_4:+74766388 | Msa0702920:CDS |
| TGGACTAAGATACCAAAACT+GGG | 0.618651 | 4_4:+74765905 | Msa0702920:CDS |
| GAACAATAAGGCCTCTTCTG+GGG | 0.622845 | 4_4:-74766320 | None:intergenic |
| AAAGAACTTGTTACGAACAG+TGG | 0.644687 | 4_4:+74765966 | Msa0702920:CDS |
| AGTCTACGGTCTCTCCAAAG+AGG | 0.659563 | 4_4:+74766206 | Msa0702920:CDS |
| GGACTAAGATACCAAAACTG+GGG | 0.731002 | 4_4:+74765906 | Msa0702920:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGTAAAGAAAAGAGTGAAAA+TGG | - | chr4_4:74766080-74766099 | None:intergenic | 25.0% |
| ! | TTCATACCTTCAAATGAAAT+TGG | + | chr4_4:74766358-74766377 | Msa0702920:CDS | 25.0% |
| ATCTGAATCAGAAACATCAA+TGG | - | chr4_4:74766030-74766049 | None:intergenic | 30.0% | |
| ! | GATGTTTCTGATTCAGATAA+TGG | + | chr4_4:74766032-74766051 | Msa0702920:CDS | 30.0% |
| TTCATATTCATGCAAGTCTA+CGG | + | chr4_4:74766192-74766211 | Msa0702920:CDS | 30.0% | |
| ! | GTTCACATTTTCGAACAATA+AGG | - | chr4_4:74766335-74766354 | None:intergenic | 30.0% |
| TGGACTAAGATACCAAAACT+GGG | + | chr4_4:74765905-74765924 | Msa0702920:CDS | 35.0% | |
| TTTGGAAAACAACGTTTGAG+AGG | - | chr4_4:74765948-74765967 | None:intergenic | 35.0% | |
| ! | CTGTTCGTAACAAGTTCTTT+TGG | - | chr4_4:74765966-74765985 | None:intergenic | 35.0% |
| !! | AAAGAACTTGTTACGAACAG+TGG | + | chr4_4:74765966-74765985 | Msa0702920:CDS | 35.0% |
| !! | TGCTATATACTACTTTTGCG+AGG | + | chr4_4:74766101-74766120 | Msa0702920:intron | 35.0% |
| !! | GCTATATACTACTTTTGCGA+GGG | + | chr4_4:74766102-74766121 | Msa0702920:intron | 35.0% |
| GAGGATTATCAATCAGAAGA+TGG | + | chr4_4:74766237-74766256 | Msa0702920:CDS | 35.0% | |
| CCAAGACCAATTTCATTTGA+AGG | - | chr4_4:74766367-74766386 | None:intergenic | 35.0% | |
| ! | CCTTCAAATGAAATTGGTCT+TGG | + | chr4_4:74766364-74766383 | Msa0702920:CDS | 35.0% |
| ! | CATAAAACTGGCTTCTCTTC+TGG | - | chr4_4:74765876-74765895 | None:intergenic | 40.0% |
| CCACATGCATTGCATAAAAC+TGG | - | chr4_4:74765888-74765907 | None:intergenic | 40.0% | |
| !! | CCAGTTTTATGCAATGCATG+TGG | + | chr4_4:74765885-74765904 | Msa0702920:CDS | 40.0% |
| ! | GTGGACTAAGATACCAAAAC+TGG | + | chr4_4:74765904-74765923 | Msa0702920:CDS | 40.0% |
| GGACTAAGATACCAAAACTG+GGG | + | chr4_4:74765906-74765925 | Msa0702920:CDS | 40.0% | |
| ! | CTTCAAGATTTCCCCAGTTT+TGG | - | chr4_4:74765920-74765939 | None:intergenic | 40.0% |
| !! | ATATACTACTTTTGCGAGGG+TGG | + | chr4_4:74766105-74766124 | Msa0702920:intron | 40.0% |
| !! | TATACTACTTTTGCGAGGGT+GGG | + | chr4_4:74766106-74766125 | Msa0702920:intron | 40.0% |
| GCAGTTCTTCTTAAACCAGA+CGG | + | chr4_4:74766388-74766407 | Msa0702920:CDS | 40.0% | |
| ! | TTTTTCCAGCAACTCCAGCA+TGG | + | chr4_4:74765841-74765860 | Msa0702920:CDS | 45.0% |
| ATCCTCAAACTACGCCTCTT+TGG | - | chr4_4:74766223-74766242 | None:intergenic | 45.0% | |
| GAACAATAAGGCCTCTTCTG+GGG | - | chr4_4:74766323-74766342 | None:intergenic | 45.0% | |
| CGAACAATAAGGCCTCTTCT+GGG | - | chr4_4:74766324-74766343 | None:intergenic | 45.0% | |
| TCGAACAATAAGGCCTCTTC+TGG | - | chr4_4:74766325-74766344 | None:intergenic | 45.0% | |
| AGTCTACGGTCTCTCCAAAG+AGG | + | chr4_4:74766206-74766225 | Msa0702920:CDS | 50.0% | |
| ! | CTCCAAAGAGGCGTAGTTTG+AGG | + | chr4_4:74766218-74766237 | Msa0702920:CDS | 50.0% |
| ! | TCTTCTGGGGATGACTCATC+TGG | - | chr4_4:74766310-74766329 | None:intergenic | 50.0% |
| AGATGAGTCATCCCCAGAAG+AGG | + | chr4_4:74766309-74766328 | Msa0702920:CDS | 50.0% | |
| CTGGCTTCTCTTCTGGTCCC+TGG | - | chr4_4:74765869-74765888 | None:intergenic | 60.0% | |
| TGGCGCCATGCTGGAGTTGC+TGG | - | chr4_4:74765849-74765868 | None:intergenic | 65.0% | |
| AGCAACTCCAGCATGGCGCC+AGG | + | chr4_4:74765848-74765867 | Msa0702920:CDS | 65.0% | |
| GCAACTCCAGCATGGCGCCA+GGG | + | chr4_4:74765849-74765868 | Msa0702920:CDS | 65.0% | |
| TCTGGTCCCTGGCGCCATGC+TGG | - | chr4_4:74765858-74765877 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 74765837 | 74766423 | 74765837 | ID=Msa0702920;Name=Msa0702920 |
| chr4_4 | mRNA | 74765837 | 74766423 | 74765837 | ID=Msa0702920-mRNA-1;Parent=Msa0702920;Name=Msa0702920-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|152 |
| chr4_4 | exon | 74765837 | 74766053 | 74765837 | ID=Msa0702920-mRNA-1:exon:23302;Parent=Msa0702920-mRNA-1 |
| chr4_4 | exon | 74766182 | 74766423 | 74766182 | ID=Msa0702920-mRNA-1:exon:23303;Parent=Msa0702920-mRNA-1 |
| chr4_4 | CDS | 74765837 | 74766053 | 74765837 | ID=Msa0702920-mRNA-1:cds;Parent=Msa0702920-mRNA-1 |
| chr4_4 | CDS | 74766182 | 74766423 | 74766182 | ID=Msa0702920-mRNA-1:cds;Parent=Msa0702920-mRNA-1 |
| Gene Sequence |
| Protein sequence |
