AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0705070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0705070 MtrunA17_Chr8g0383251 95.109 184 8 1 1 183 1 184 3.76e-128 357
Msa0705070 MtrunA17_Chr3g0129321 52.041 196 77 5 1 182 1 193 4.94e-56 175
Msa0705070 MtrunA17_Chr1g0150801 47.518 141 72 1 23 163 20 158 9.99e-37 125
Msa0705070 MtrunA17_Chr2g0294231 58.427 89 36 1 90 178 186 273 1.27e-30 114
Msa0705070 MtrunA17_Chr7g0218081 42.975 121 56 1 65 172 355 475 1.14e-28 110
Msa0705070 MtrunA17_Chr7g0218081 56.667 60 25 1 107 166 228 286 2.19e-14 70.5
Msa0705070 MtrunA17_Chr7g0270191 46.957 115 54 2 65 174 100 212 4.90e-28 106
Msa0705070 MtrunA17_Chr4g0051881 58.108 74 31 0 93 166 164 237 1.02e-27 106
Msa0705070 MtrunA17_Chr1g0194481 47.475 99 52 0 74 172 125 223 1.15e-27 105
Msa0705070 MtrunA17_Chr4g0004911 39.850 133 78 1 35 167 266 396 2.87e-27 107
Msa0705070 MtrunA17_Chr4g0004911 50.746 67 33 0 100 166 177 243 2.92e-16 75.9
Msa0705070 MtrunA17_Chr5g0443131 59.211 76 31 0 93 168 143 218 6.53e-27 103
Msa0705070 MtrunA17_Chr7g0243891 37.870 169 88 5 9 166 271 433 9.67e-27 105
Msa0705070 MtrunA17_Chr7g0243891 52.308 65 30 1 102 166 211 274 4.61e-15 72.4
Msa0705070 MtrunA17_Chr5g0418191 58.108 74 31 0 93 166 306 379 1.08e-26 104
Msa0705070 MtrunA17_Chr5g0418191 53.333 60 27 1 107 166 151 209 7.52e-13 65.9
Msa0705070 MtrunA17_Chr3g0079691 52.439 82 39 0 93 174 481 562 1.22e-26 105
Msa0705070 MtrunA17_Chr3g0079691 49.275 69 34 1 107 175 280 347 5.41e-13 66.2
Msa0705070 MtrunA17_Chr4g0042831 46.078 102 42 1 78 166 236 337 1.44e-26 103
Msa0705070 MtrunA17_Chr4g0042831 49.206 63 32 0 107 169 145 207 1.86e-12 64.7
Msa0705070 MtrunA17_Chr3g0101131 41.045 134 66 2 52 172 347 480 1.46e-26 105
Msa0705070 MtrunA17_Chr3g0101131 49.206 63 31 1 107 169 243 304 6.55e-11 60.5
Msa0705070 MtrunA17_Chr7g0249311 51.807 83 40 0 93 175 378 460 1.73e-26 105
Msa0705070 MtrunA17_Chr7g0249311 52.381 63 29 1 107 169 179 240 5.36e-13 66.2
Msa0705070 MtrunA17_Chr5g0445311 47.000 100 51 1 69 166 324 423 1.92e-26 104
Msa0705070 MtrunA17_Chr5g0445311 50.000 60 29 1 107 166 200 258 7.19e-11 60.1
Msa0705070 MtrunA17_Chr7g0224801 56.757 74 32 0 96 169 82 155 2.51e-26 98.6
Msa0705070 MtrunA17_Chr5g0418781 48.598 107 46 2 57 163 83 180 2.76e-26 101
Msa0705070 MtrunA17_Chr7g0249301 51.807 83 39 1 84 166 270 351 3.31e-26 102
Msa0705070 MtrunA17_Chr7g0249301 50.000 64 31 1 106 169 61 123 1.97e-12 64.3
Msa0705070 MtrunA17_Chr8g0335811 44.538 119 58 3 51 163 94 210 8.95e-26 99.0
Msa0705070 MtrunA17_Chr3g0106491 54.054 74 34 0 93 166 351 424 1.61e-24 99.4
Msa0705070 MtrunA17_Chr3g0106491 45.946 74 35 2 97 166 184 256 3.15e-14 69.7
Msa0705070 MtrunA17_Chr4g0068541 48.810 84 43 0 94 177 195 278 2.55e-24 98.2
Msa0705070 MtrunA17_Chr7g0248721 47.059 102 48 2 83 179 117 217 2.81e-24 94.7
Msa0705070 MtrunA17_Chr5g0432161 54.054 74 34 0 93 166 407 480 7.15e-24 97.8
Msa0705070 MtrunA17_Chr5g0432161 53.333 60 27 1 107 166 244 302 1.80e-13 67.8
Msa0705070 MtrunA17_Chr3g0129361 54.930 71 32 0 93 163 130 200 1.96e-23 92.4
Msa0705070 MtrunA17_Chr7g0263531 49.333 75 38 0 93 167 347 421 1.71e-22 93.2
Msa0705070 MtrunA17_Chr7g0263531 54.237 59 26 1 108 166 190 247 3.95e-12 63.5
Msa0705070 MtrunA17_Chr5g0447701 54.795 73 33 0 94 166 239 311 3.50e-22 92.0
Msa0705070 MtrunA17_Chr1g0189391 53.947 76 34 1 89 163 79 154 5.66e-22 90.9
Msa0705070 MtrunA17_Chr8g0386241 51.389 72 35 0 94 165 90 161 6.79e-22 87.4
Msa0705070 MtrunA17_Chr4g0074001 48.148 81 42 0 87 167 339 419 1.09e-21 91.3
Msa0705070 MtrunA17_Chr8g0383351 54.167 72 32 1 93 163 145 216 1.13e-21 88.2
Msa0705070 MtrunA17_Chr6g0485431 45.122 82 44 1 89 169 136 217 4.17e-21 88.6
Msa0705070 MtrunA17_Chr1g0188941 47.297 74 39 0 93 166 37 110 6.37e-21 83.2
Msa0705070 MtrunA17_Chr7g0270831 47.297 74 38 1 94 166 203 276 8.48e-21 88.6
Msa0705070 MtrunA17_Chr4g0075201 45.946 74 39 1 94 166 254 327 4.71e-20 86.7
Msa0705070 MtrunA17_Chr4g0051181 48.000 75 38 1 94 167 230 304 1.85e-19 84.7
Msa0705070 MtrunA17_Chr7g0238921 55.932 59 26 0 105 163 122 180 2.74e-19 83.2
Msa0705070 MtrunA17_Chr3g0099641 44.156 77 42 1 94 169 238 314 3.54e-19 84.0
Msa0705070 MtrunA17_Chr2g0322391 44.595 74 40 1 94 166 336 409 1.60e-18 82.4
Msa0705070 MtrunA17_Chr2g0302391 47.297 74 38 1 94 166 198 271 1.62e-18 82.0
Msa0705070 MtrunA17_Chr4g0053281 50.000 74 33 2 93 165 255 325 2.45e-18 80.9
Msa0705070 MtrunA17_Chr1g0147881 47.191 89 39 4 79 165 239 321 3.37e-18 80.5
Msa0705070 MtrunA17_Chr4g0060271 44.000 75 41 1 94 167 242 316 8.48e-18 80.1
Msa0705070 MtrunA17_Chr5g0401821 42.683 82 46 1 85 165 177 258 1.05e-17 78.2
Msa0705070 MtrunA17_Chr2g0288871 45.946 74 39 1 94 166 292 365 2.01e-17 79.0
Msa0705070 MtrunA17_Chr7g0216891 42.857 77 43 1 94 169 206 282 2.07e-17 79.0
Msa0705070 MtrunA17_Chr4g0025051 45.455 77 41 1 94 169 322 398 2.07e-17 79.0
Msa0705070 MtrunA17_Chr2g0333851 47.368 76 37 2 94 166 154 229 3.45e-17 77.4
Msa0705070 MtrunA17_Chr3g0136961 50.847 59 28 1 106 163 137 195 1.40e-16 75.5
Msa0705070 MtrunA17_Chr5g0447711 42.683 82 46 1 86 166 208 289 2.14e-16 75.1
Msa0705070 MtrunA17_Chr2g0293481 37.755 98 59 2 71 166 230 327 5.56e-16 75.1
Msa0705070 MtrunA17_Chr4g0001361 50.794 63 30 1 105 166 160 222 7.63e-16 73.9
Msa0705070 MtrunA17_Chr1g0184691 52.459 61 28 1 104 163 290 350 1.28e-15 73.6
Msa0705070 MtrunA17_Chr8g0379461 44.286 70 38 1 108 176 77 146 1.83e-15 72.0
Msa0705070 MtrunA17_Chr4g0025061 41.558 77 44 1 94 169 347 423 3.00e-15 72.8
Msa0705070 MtrunA17_Chr4g0071521 50.820 61 29 1 104 163 242 302 6.30e-15 71.2
Msa0705070 MtrunA17_Chr2g0319961 43.662 71 39 1 98 167 71 141 6.39e-15 70.5
Msa0705070 MtrunA17_Chr7g0270051 50.000 60 29 1 108 166 54 113 1.17e-14 70.1
Msa0705070 MtrunA17_Chr8g0348311 46.875 64 33 1 104 166 152 215 2.68e-14 69.7
Msa0705070 MtrunA17_Chr7g0228941 33.636 110 67 3 56 164 6 110 3.65e-14 65.9
Msa0705070 MtrunA17_Chr1g0150281 45.614 57 30 1 108 163 141 197 8.69e-14 67.8
Msa0705070 MtrunA17_Chr7g0248691 47.619 63 32 1 105 166 152 214 9.69e-14 68.2
Msa0705070 MtrunA17_Chr1g0202461 46.032 63 33 1 105 166 259 321 2.19e-13 67.4
Msa0705070 MtrunA17_Chr2g0289181 43.939 66 36 1 102 166 9 74 8.10e-13 64.3
Msa0705070 MtrunA17_Chr5g0432071 50.794 63 30 1 107 169 104 165 1.09e-12 63.9
Msa0705070 MtrunA17_Chr2g0284321 45.000 60 32 1 108 166 36 95 1.54e-12 63.5
Msa0705070 MtrunA17_Chr1g0150271 41.270 63 36 1 105 166 99 161 1.60e-12 63.9
Msa0705070 MtrunA17_Chr8g0387501 46.667 60 31 1 108 166 130 189 1.89e-12 63.9
Msa0705070 MtrunA17_Chr3g0139021 44.262 61 33 1 104 163 311 371 2.42e-12 64.3
Msa0705070 MtrunA17_Chr4g0024391 37.662 77 47 1 94 169 216 292 8.17e-12 62.8
Msa0705070 MtrunA17_Chr5g0402931 45.000 60 31 1 104 163 120 177 1.79e-11 60.1
Msa0705070 MtrunA17_Chr3g0126391 32.381 105 57 4 63 161 94 190 4.96e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0705070 AT5G64810.1 76.000 75 18 0 90 164 93 167 3.04e-38 130
Msa0705070 AT5G26170.1 75.714 70 17 0 94 163 100 169 1.46e-35 122
Msa0705070 AT5G49520.1 60.000 75 30 0 94 168 208 282 9.47e-29 110
Msa0705070 AT4G18170.1 56.250 80 35 0 87 166 152 231 2.64e-28 107
Msa0705070 AT1G29860.1 61.333 75 29 0 94 168 123 197 3.62e-28 106
Msa0705070 AT5G46350.1 49.451 91 46 0 93 183 169 259 1.15e-27 106
Msa0705070 AT3G62340.1 61.333 75 29 0 93 167 104 178 3.50e-27 103
Msa0705070 AT3G01970.1 55.556 72 32 0 94 165 52 123 7.53e-27 99.8
Msa0705070 AT2G38470.1 56.757 74 32 0 93 166 348 421 3.03e-26 104
Msa0705070 AT2G38470.1 51.667 60 28 1 107 166 184 242 1.82e-12 65.1
Msa0705070 AT1G69310.4 54.118 85 36 1 81 165 124 205 3.38e-26 101
Msa0705070 AT1G69310.2 54.118 85 36 1 81 165 124 205 3.38e-26 101
Msa0705070 AT1G69310.1 54.118 85 36 1 81 165 124 205 3.38e-26 101
Msa0705070 AT2G46130.1 55.128 78 35 0 86 163 9 86 7.25e-26 95.9
Msa0705070 AT5G13080.1 56.944 72 31 0 94 165 54 125 1.21e-25 96.7
Msa0705070 AT5G56270.1 55.405 74 33 0 93 166 473 546 2.52e-25 102
Msa0705070 AT5G56270.1 53.333 60 27 1 107 166 273 331 6.88e-13 66.6
Msa0705070 AT3G01080.1 55.405 74 33 0 93 166 292 365 2.57e-25 101
Msa0705070 AT3G01080.1 53.333 60 27 1 107 166 167 225 3.44e-13 67.0
Msa0705070 AT3G01080.2 55.405 74 33 0 93 166 330 403 2.64e-25 101
Msa0705070 AT3G01080.2 53.333 60 27 1 107 166 205 263 3.19e-13 67.4
Msa0705070 AT1G13960.2 54.054 74 34 0 93 166 242 315 2.70e-25 100
Msa0705070 AT1G13960.2 50.667 75 33 2 95 166 61 134 6.43e-16 74.7
Msa0705070 AT5G41570.1 52.500 80 38 0 84 163 75 154 3.07e-25 96.7
Msa0705070 AT5G07100.2 55.405 74 33 0 93 166 127 200 3.26e-25 97.4
Msa0705070 AT5G07100.2 48.333 60 31 0 107 166 24 83 4.73e-12 62.4
Msa0705070 AT5G07100.4 55.405 74 33 0 93 166 127 200 3.26e-25 97.4
Msa0705070 AT5G07100.4 48.333 60 31 0 107 166 24 83 4.73e-12 62.4
Msa0705070 AT5G07100.5 55.405 74 33 0 93 166 127 200 3.26e-25 97.4
Msa0705070 AT5G07100.5 48.333 60 31 0 107 166 24 83 4.73e-12 62.4
Msa0705070 AT5G07100.3 55.405 74 33 0 93 166 127 200 3.26e-25 97.4
Msa0705070 AT5G07100.3 48.333 60 31 0 107 166 24 83 4.73e-12 62.4
Msa0705070 AT1G13960.1 54.054 74 34 0 93 166 395 468 4.34e-25 101
Msa0705070 AT1G13960.1 50.667 75 33 2 95 166 214 287 4.69e-16 75.5
Msa0705070 AT2G44745.1 47.368 114 56 3 54 166 93 203 4.59e-25 97.1
Msa0705070 AT4G26640.5 51.250 80 39 0 93 172 191 270 5.78e-25 99.8
Msa0705070 AT4G26640.5 50.000 62 30 1 105 166 33 93 2.30e-11 62.0
Msa0705070 AT4G26640.6 51.250 80 39 0 93 172 191 270 5.78e-25 99.8
Msa0705070 AT4G26640.6 50.000 62 30 1 105 166 33 93 2.30e-11 62.0
Msa0705070 AT4G26640.4 51.250 80 39 0 93 172 191 270 5.78e-25 99.8
Msa0705070 AT4G26640.4 50.000 62 30 1 105 166 33 93 2.30e-11 62.0
Msa0705070 AT4G26640.3 51.250 80 39 0 93 172 191 270 5.78e-25 99.8
Msa0705070 AT4G26640.3 50.000 62 30 1 105 166 33 93 2.30e-11 62.0
Msa0705070 AT5G07100.1 55.405 74 33 0 93 166 220 293 6.07e-25 98.6
Msa0705070 AT5G07100.1 48.333 60 31 0 107 166 117 176 3.33e-12 63.9
Msa0705070 AT2G47260.1 35.119 168 82 3 2 169 96 236 6.22e-25 99.4
Msa0705070 AT4G26640.1 51.250 80 39 0 93 172 295 374 8.72e-25 100
Msa0705070 AT4G26640.1 50.000 62 30 1 105 166 137 197 1.40e-11 62.4
Msa0705070 AT4G26640.7 51.250 80 39 0 93 172 327 406 8.73e-25 100
Msa0705070 AT4G26640.7 50.000 62 30 1 105 166 169 229 1.26e-11 62.8
Msa0705070 AT1G64000.1 53.659 82 38 0 82 163 89 170 9.55e-25 95.5
Msa0705070 AT2G21900.1 47.917 96 46 2 85 176 83 178 1.00e-24 95.9
Msa0705070 AT4G26640.2 51.250 80 39 0 93 172 367 446 1.04e-24 100
Msa0705070 AT4G26640.2 50.000 62 30 1 105 166 209 269 1.32e-11 62.8
Msa0705070 AT2G03340.1 52.703 74 35 0 93 166 401 474 6.64e-24 97.8
Msa0705070 AT2G03340.1 56.667 60 25 1 107 166 250 308 3.98e-15 72.8
Msa0705070 AT4G26440.1 48.193 83 43 0 93 175 358 440 1.33e-23 97.4
Msa0705070 AT4G26440.1 51.667 60 28 1 107 166 178 236 3.17e-11 61.6
Msa0705070 AT4G39410.1 52.326 86 40 1 78 163 195 279 1.52e-23 95.1
Msa0705070 AT2G04880.2 42.857 112 59 2 58 166 233 342 2.23e-23 96.3
Msa0705070 AT2G04880.2 43.750 64 35 1 104 167 108 170 3.90e-11 61.2
Msa0705070 AT2G04880.1 42.857 112 59 2 58 166 257 366 2.35e-23 96.3
Msa0705070 AT2G04880.1 42.424 66 37 1 104 169 108 172 3.74e-11 61.2
Msa0705070 AT2G30250.1 49.398 83 39 1 87 166 305 387 1.74e-22 93.2
Msa0705070 AT2G30250.1 48.649 74 36 2 107 179 166 238 4.40e-11 60.8
Msa0705070 AT1G69310.3 50.588 85 35 2 81 165 124 201 1.80e-22 91.7
Msa0705070 AT2G46130.2 52.564 78 33 1 86 163 9 82 2.41e-22 87.0
Msa0705070 AT4G04450.2 47.297 74 38 1 94 166 185 258 2.15e-20 87.8
Msa0705070 AT5G43290.1 62.712 59 22 0 105 163 112 170 3.64e-20 85.5
Msa0705070 AT1G55600.1 43.529 85 48 0 82 166 282 366 5.32e-20 86.7
Msa0705070 AT4G04450.1 47.297 74 38 1 94 166 279 352 6.18e-20 86.7
Msa0705070 AT1G68150.1 46.667 75 39 1 94 167 222 296 1.83e-19 84.7
Msa0705070 AT4G30935.1 45.946 74 40 0 94 167 318 391 3.08e-19 84.7
Msa0705070 AT1G62300.1 47.297 74 38 1 94 166 299 372 3.33e-19 84.7
Msa0705070 AT5G15130.2 42.222 90 45 2 81 169 165 248 4.44e-19 84.3
Msa0705070 AT5G15130.1 42.222 90 45 2 81 169 207 290 4.60e-19 84.3
Msa0705070 AT4G22070.4 47.297 74 38 1 94 166 284 357 6.03e-19 84.0
Msa0705070 AT4G22070.3 47.297 74 38 1 94 166 284 357 6.03e-19 84.0
Msa0705070 AT4G22070.2 47.297 74 38 1 94 166 284 357 6.03e-19 84.0
Msa0705070 AT4G22070.1 47.297 74 38 1 94 166 284 357 6.03e-19 84.0
Msa0705070 AT2G23320.1 47.674 86 41 2 81 165 217 299 6.10e-19 82.8
Msa0705070 AT4G01720.1 44.595 74 40 1 94 166 226 299 1.94e-18 82.4
Msa0705070 AT1G18860.1 44.595 74 40 1 94 166 226 299 1.24e-17 80.1
Msa0705070 AT4G24240.1 47.778 90 37 4 79 165 258 340 1.97e-17 79.0
Msa0705070 AT3G04670.1 46.250 80 42 1 85 163 240 319 2.77e-17 78.2
Msa0705070 AT5G28650.1 39.837 123 59 4 42 163 211 319 4.52e-17 77.8
Msa0705070 AT1G29280.1 50.794 63 30 1 108 169 75 137 5.18e-17 76.6
Msa0705070 AT2G25000.2 47.692 65 33 1 106 169 102 166 6.26e-17 75.9
Msa0705070 AT2G25000.4 47.692 65 33 1 106 169 102 166 6.26e-17 75.9
Msa0705070 AT2G25000.3 47.692 65 33 1 106 169 124 188 1.05e-16 75.9
Msa0705070 AT2G24570.1 42.342 111 48 5 62 163 197 300 1.11e-16 76.6
Msa0705070 AT1G80840.1 46.032 63 33 1 105 166 144 206 1.42e-16 75.9
Msa0705070 AT4G31550.2 47.727 88 36 4 79 163 222 302 1.63e-16 76.3
Msa0705070 AT2G25000.1 47.692 65 33 1 106 169 145 209 1.79e-16 75.5
Msa0705070 AT4G31550.1 47.674 86 35 4 81 163 225 303 4.34e-16 75.1
Msa0705070 AT2G34830.1 42.391 92 45 3 83 166 184 275 6.98e-16 75.1
Msa0705070 AT2G34830.2 42.391 92 45 3 83 166 193 284 7.04e-16 75.1
Msa0705070 AT3G58710.2 36.508 126 73 3 51 169 8 133 2.01e-15 72.4
Msa0705070 AT4G31800.2 43.939 66 36 1 106 170 110 175 2.13e-15 72.0
Msa0705070 AT1G30650.1 40.860 93 47 3 83 167 186 278 2.54e-15 73.2
Msa0705070 AT4G31800.1 43.939 66 36 1 106 170 175 240 3.88e-15 72.0
Msa0705070 AT2G37260.3 46.875 64 34 0 102 165 264 327 6.17e-15 72.0
Msa0705070 AT2G37260.3 41.237 97 48 3 78 166 48 143 6.34e-13 66.2
Msa0705070 AT2G37260.2 46.875 64 34 0 102 165 262 325 6.29e-15 72.0
Msa0705070 AT2G37260.2 41.237 97 48 3 78 166 46 141 6.67e-13 66.2
Msa0705070 AT2G37260.1 46.875 64 34 0 102 165 344 407 6.40e-15 72.0
Msa0705070 AT2G37260.1 41.237 97 48 3 78 166 128 223 7.32e-13 66.2
Msa0705070 AT3G58710.1 45.205 73 39 1 98 169 62 134 9.26e-15 70.9
Msa0705070 AT2G30590.1 52.459 61 28 1 104 163 310 370 1.04e-14 71.2
Msa0705070 AT4G01250.1 50.000 60 29 1 108 166 129 188 8.48e-14 68.2
Msa0705070 AT3G01080.3 53.333 60 27 1 107 166 205 263 1.60e-13 67.8
Msa0705070 AT4G23550.1 43.939 66 36 1 102 166 135 200 1.82e-13 67.4
Msa0705070 AT1G69810.2 37.079 89 48 3 81 167 125 207 4.07e-13 66.6
Msa0705070 AT1G69810.1 37.079 89 48 3 81 167 183 265 6.27e-13 66.2
Msa0705070 AT5G52830.1 47.619 63 32 1 105 166 163 225 1.35e-12 65.1
Msa0705070 AT5G45050.4 48.333 60 30 1 108 166 1042 1101 3.55e-12 64.7
Msa0705070 AT5G45050.1 48.333 60 30 1 108 166 1181 1240 4.02e-12 64.3
Msa0705070 AT5G45050.2 48.333 60 30 1 108 166 1153 1212 4.21e-12 64.3
Msa0705070 AT1G66560.1 36.667 90 45 3 97 183 93 173 4.17e-11 60.5

Find 45 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATAGATAAACTCAGTTT+TGG 0.232272 4_4:-77598322 None:intergenic
AAGATCAGAGCTTGAGATTA+TGG 0.284446 4_4:+77599033 Msa0705070:CDS
CTTGTTCTAAAGGCTATCTT+TGG 0.312484 4_4:-77599013 None:intergenic
GTAAACTCAGAAGAGGAATT+AGG 0.336749 4_4:-77598408 None:intergenic
CAATTTGAGAGGGAGTTAAA+TGG 0.346614 4_4:-77600409 None:intergenic
CAGAATGCACAAAAGAGATT+TGG 0.356317 4_4:-77600331 None:intergenic
TGAGTAGAGTGAGCAAAGTT+TGG 0.356689 4_4:-77598378 None:intergenic
TGCATCATTGAATTCTTGAT+TGG 0.365316 4_4:-77598535 None:intergenic
GAGTAGAGTGAGCAAAGTTT+GGG 0.391273 4_4:-77598377 None:intergenic
TTACATATTATTGTTCTTTG+AGG 0.400718 4_4:-77598559 None:intergenic
TGGCTGCAGTGGGAGGTAAA+TGG 0.416069 4_4:-77600311 None:intergenic
CAAAAGAGATTTGGCTGCAG+TGG 0.429261 4_4:-77600322 None:intergenic
TGTTACCTTCTCTAATGATT+CGG 0.455426 4_4:-77598502 None:intergenic
TGTGAAGAAAAGGGTGGAAA+GGG 0.465962 4_4:+77600229 Msa0705070:CDS
AAGCTCTGATCTTGTTCTAA+AGG 0.476991 4_4:-77599023 None:intergenic
GGATACAAGTGGAGGAAGTA+CGG 0.476991 4_4:+77599061 Msa0705070:CDS
TGGGATGTAATGTGAAGAAA+AGG 0.500798 4_4:+77600219 Msa0705070:CDS
GTGACCGAGATTCTTGATCA+AGG 0.501478 4_4:-77598473 None:intergenic
AAGCGAATTAAAGTAGAAGC+GGG 0.522259 4_4:+77598989 Msa0705070:CDS
GTGAGAGAATACAATTTGAG+AGG 0.523214 4_4:-77600420 None:intergenic
AACTGAGTTTATCTATGCAA+TGG 0.530683 4_4:+77598326 Msa0705070:exon
ATGTGAAGAAAAGGGTGGAA+AGG 0.539725 4_4:+77600228 Msa0705070:CDS
CAAAGTTTGGGGTAGCATGA+GGG 0.552088 4_4:-77598365 None:intergenic
GATACAAGTGGAGGAAGTAC+GGG 0.554483 4_4:+77599062 Msa0705070:CDS
ATCACCTTGATCAAGAATCT+CGG 0.560454 4_4:+77598469 Msa0705070:CDS
TGAAGAACAGTCCTAACCTA+AGG 0.563814 4_4:+77599095 Msa0705070:CDS
TATGTGATAACAAGTTATGA+AGG 0.564114 4_4:+77600269 Msa0705070:CDS
TACTACAAATGTTCAAGTGT+GGG 0.566302 4_4:+77600200 Msa0705070:CDS
TGAGAGAATACAATTTGAGA+GGG 0.572606 4_4:-77600419 None:intergenic
GCAAAGTTTGGGGTAGCATG+AGG 0.576767 4_4:-77598366 None:intergenic
AAAAGAGATTTGGCTGCAGT+GGG 0.577682 4_4:-77600321 None:intergenic
AGATAGTGTAAACTCAGAAG+AGG 0.584693 4_4:-77598415 None:intergenic
CTACTACAAATGTTCAAGTG+TGG 0.597033 4_4:+77600199 Msa0705070:CDS
AAGTACCGAATCATTAGAGA+AGG 0.606756 4_4:+77598497 Msa0705070:CDS
GGGAGGTAAATGGAATCTCA+TGG 0.608064 4_4:-77600301 None:intergenic
AGTAGAGTGAGCAAAGTTTG+GGG 0.609244 4_4:-77598376 None:intergenic
AGAGATTTGGCTGCAGTGGG+AGG 0.615268 4_4:-77600318 None:intergenic
GAGCTTGAGATTATGGACGA+TGG 0.615286 4_4:+77599040 Msa0705070:CDS
TTATGGACGATGGATACAAG+TGG 0.616058 4_4:+77599050 Msa0705070:CDS
TAAGCGAATTAAAGTAGAAG+CGG 0.631449 4_4:+77598988 Msa0705070:CDS
GGGATGTAATGTGAAGAAAA+GGG 0.671707 4_4:+77600220 Msa0705070:CDS
ATGTAATGTGAAGAAAAGGG+TGG 0.682851 4_4:+77600223 Msa0705070:CDS
TGGACGATGGATACAAGTGG+AGG 0.691711 4_4:+77599053 Msa0705070:CDS
AGCGAATTAAAGTAGAAGCG+GGG 0.700406 4_4:+77598990 Msa0705070:CDS
AGTATGTATCATGTATGCAG+TGG 0.744278 4_4:-77600372 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCATTTAAAATTAAGAAAA+GGG + chr4_4:77598613-77598632 Msa0705070:intron 10.0%
!!! TTCATTTAAAATTAAGAAAA+GGG + chr4_4:77598613-77598632 Msa0705070:intron 10.0%
!!! GTTCATTTAAAATTAAGAAA+AGG + chr4_4:77598612-77598631 Msa0705070:intron 15.0%
!! CAAATACAAAATGAAATTTA+AGG - chr4_4:77599161-77599180 None:intergenic 15.0%
!!! TTGAAATTCAATTTTTAACT+CGG + chr4_4:77599443-77599462 Msa0705070:intron 15.0%
!! AAGTCATAAACAAAAAAAAT+TGG - chr4_4:77599470-77599489 None:intergenic 15.0%
!! TTTAATGAAATTTCAAACTA+GGG - chr4_4:77599987-77600006 None:intergenic 15.0%
!!! GTTCATTTAAAATTAAGAAA+AGG + chr4_4:77598612-77598631 Msa0705070:intron 15.0%
!! CAAATACAAAATGAAATTTA+AGG - chr4_4:77599161-77599180 None:intergenic 15.0%
!!! TTGAAATTCAATTTTTAACT+CGG + chr4_4:77599443-77599462 Msa0705070:intron 15.0%
!! AAGTCATAAACAAAAAAAAT+TGG - chr4_4:77599470-77599489 None:intergenic 15.0%
!! TTTAATGAAATTTCAAACTA+GGG - chr4_4:77599987-77600006 None:intergenic 15.0%
!! TTACATATTATTGTTCTTTG+AGG - chr4_4:77598562-77598581 None:intergenic 20.0%
!!! CTAATAAAGTGTACATATTT+TGG + chr4_4:77598922-77598941 Msa0705070:intron 20.0%
!!! TTTTGCAGAAAATACAAAAA+TGG + chr4_4:77598962-77598981 Msa0705070:intron 20.0%
!! TTTGCAGAAAATACAAAAAT+GGG + chr4_4:77598963-77598982 Msa0705070:intron 20.0%
!! TAACTATAAGTTCATGATAT+TGG + chr4_4:77599283-77599302 Msa0705070:intron 20.0%
!! AAAACGATGAAAATGAATAT+TGG + chr4_4:77599338-77599357 Msa0705070:intron 20.0%
!! TGTCTTAACATAAATTAGTT+AGG + chr4_4:77599948-77599967 Msa0705070:intron 20.0%
!! CTTTAATGAAATTTCAAACT+AGG - chr4_4:77599988-77600007 None:intergenic 20.0%
!! AAACATATAAAGTCAAAACA+AGG - chr4_4:77600073-77600092 None:intergenic 20.0%
!! TTACATATTATTGTTCTTTG+AGG - chr4_4:77598562-77598581 None:intergenic 20.0%
!!! CTAATAAAGTGTACATATTT+TGG + chr4_4:77598922-77598941 Msa0705070:intron 20.0%
!!! TTTTGCAGAAAATACAAAAA+TGG + chr4_4:77598962-77598981 Msa0705070:intron 20.0%
!! TTTGCAGAAAATACAAAAAT+GGG + chr4_4:77598963-77598982 Msa0705070:intron 20.0%
!! TAACTATAAGTTCATGATAT+TGG + chr4_4:77599283-77599302 Msa0705070:intron 20.0%
!! AAAACGATGAAAATGAATAT+TGG + chr4_4:77599338-77599357 Msa0705070:intron 20.0%
!! TGTCTTAACATAAATTAGTT+AGG + chr4_4:77599948-77599967 Msa0705070:intron 20.0%
!! CTTTAATGAAATTTCAAACT+AGG - chr4_4:77599988-77600007 None:intergenic 20.0%
!! AAACATATAAAGTCAAAACA+AGG - chr4_4:77600073-77600092 None:intergenic 20.0%
! ACAAGAGCAATACAAATTAA+GGG - chr4_4:77598594-77598613 None:intergenic 25.0%
! AACAAGAGCAATACAAATTA+AGG - chr4_4:77598595-77598614 None:intergenic 25.0%
!! TTCAAAAGTGTACTTCTAAA+CGG - chr4_4:77598676-77598695 None:intergenic 25.0%
!!! TCTAAAAGTGTACATTTTGA+GGG + chr4_4:77598745-77598764 Msa0705070:intron 25.0%
! ATTAATGAAGCATATACCAT+CGG + chr4_4:77598857-77598876 Msa0705070:intron 25.0%
!! GTATTTCCTTTTATTACCTT+AGG - chr4_4:77599114-77599133 None:intergenic 25.0%
!! ATTTGAACAAAGTAGCTTTA+AGG + chr4_4:77599176-77599195 Msa0705070:intron 25.0%
! CTGTTTCAATAGTAAGAATA+TGG + chr4_4:77599510-77599529 Msa0705070:intron 25.0%
! AATTAGTTAGGTTTGACAAA+TGG + chr4_4:77599960-77599979 Msa0705070:intron 25.0%
! AGATGATAAAAAACGAGATT+TGG + chr4_4:77600014-77600033 Msa0705070:intron 25.0%
! TGTAGTAACTAACTAATCTT+CGG - chr4_4:77600121-77600140 None:intergenic 25.0%
! TATTGAATTAATGATGACAC+AGG + chr4_4:77600174-77600193 Msa0705070:intron 25.0%
! TATGTGATAACAAGTTATGA+AGG + chr4_4:77600269-77600288 Msa0705070:CDS 25.0%
! ACAAGAGCAATACAAATTAA+GGG - chr4_4:77598594-77598613 None:intergenic 25.0%
! AACAAGAGCAATACAAATTA+AGG - chr4_4:77598595-77598614 None:intergenic 25.0%
!! TTCAAAAGTGTACTTCTAAA+CGG - chr4_4:77598676-77598695 None:intergenic 25.0%
!!! TCTAAAAGTGTACATTTTGA+GGG + chr4_4:77598745-77598764 Msa0705070:intron 25.0%
! ATTAATGAAGCATATACCAT+CGG + chr4_4:77598857-77598876 Msa0705070:intron 25.0%
!! GTATTTCCTTTTATTACCTT+AGG - chr4_4:77599114-77599133 None:intergenic 25.0%
!! ATTTGAACAAAGTAGCTTTA+AGG + chr4_4:77599176-77599195 Msa0705070:intron 25.0%
! CTGTTTCAATAGTAAGAATA+TGG + chr4_4:77599510-77599529 Msa0705070:intron 25.0%
! AATTAGTTAGGTTTGACAAA+TGG + chr4_4:77599960-77599979 Msa0705070:intron 25.0%
! AGATGATAAAAAACGAGATT+TGG + chr4_4:77600014-77600033 Msa0705070:intron 25.0%
! TGTAGTAACTAACTAATCTT+CGG - chr4_4:77600121-77600140 None:intergenic 25.0%
! TATTGAATTAATGATGACAC+AGG + chr4_4:77600174-77600193 Msa0705070:intron 25.0%
! TATGTGATAACAAGTTATGA+AGG + chr4_4:77600269-77600288 Msa0705070:CDS 25.0%
!!! TTTTGGGTTGTTGGTATAAA+TGG - chr4_4:77598308-77598327 None:intergenic 30.0%
! GCATAGATAAACTCAGTTTT+GGG - chr4_4:77598324-77598343 None:intergenic 30.0%
! TGCATAGATAAACTCAGTTT+TGG - chr4_4:77598325-77598344 None:intergenic 30.0%
AACTGAGTTTATCTATGCAA+TGG + chr4_4:77598326-77598345 Msa0705070:exon 30.0%
TGTTACCTTCTCTAATGATT+CGG - chr4_4:77598505-77598524 None:intergenic 30.0%
TGCATCATTGAATTCTTGAT+TGG - chr4_4:77598538-77598557 None:intergenic 30.0%
!! ATCGGTTTTTAAGAAACTAC+GGG - chr4_4:77598652-77598671 None:intergenic 30.0%
!! GATCGGTTTTTAAGAAACTA+CGG - chr4_4:77598653-77598672 None:intergenic 30.0%
! TCAAAAGTGTACTTCTAAAC+GGG - chr4_4:77598675-77598694 None:intergenic 30.0%
!! GTCTAAAAGTGTACATTTTG+AGG + chr4_4:77598744-77598763 Msa0705070:intron 30.0%
!!! TGCATGATTGCATTTTTTCA+AGG - chr4_4:77598806-77598825 None:intergenic 30.0%
!! AGTGTACATATTTTGGATTG+TGG + chr4_4:77598929-77598948 Msa0705070:intron 30.0%
!! GTGTACATATTTTGGATTGT+GGG + chr4_4:77598930-77598949 Msa0705070:intron 30.0%
!! TGTACATATTTTGGATTGTG+GGG + chr4_4:77598931-77598950 Msa0705070:intron 30.0%
TAAGCGAATTAAAGTAGAAG+CGG + chr4_4:77598988-77599007 Msa0705070:CDS 30.0%
TCCTAACCTAAGGTAATAAA+AGG + chr4_4:77599105-77599124 Msa0705070:intron 30.0%
! TCCTTTTATTACCTTAGGTT+AGG - chr4_4:77599109-77599128 None:intergenic 30.0%
TTCAAACATCATAGTACGAT+AGG - chr4_4:77599222-77599241 None:intergenic 30.0%
! ATGATATTGGTCTACTCATT+TGG + chr4_4:77599296-77599315 Msa0705070:intron 30.0%
! TGATATTGGTCTACTCATTT+GGG + chr4_4:77599297-77599316 Msa0705070:intron 30.0%
AATACAATTGATGAGATGTG+TGG - chr4_4:77599642-77599661 None:intergenic 30.0%
AGTTAAACTTAACTAACCAG+TGG + chr4_4:77599832-77599851 Msa0705070:intron 30.0%
TACTACAAATGTTCAAGTGT+GGG + chr4_4:77600200-77600219 Msa0705070:CDS 30.0%
TGAGAGAATACAATTTGAGA+GGG - chr4_4:77600422-77600441 None:intergenic 30.0%
!!! TTTTGGGTTGTTGGTATAAA+TGG - chr4_4:77598308-77598327 None:intergenic 30.0%
! GCATAGATAAACTCAGTTTT+GGG - chr4_4:77598324-77598343 None:intergenic 30.0%
! TGCATAGATAAACTCAGTTT+TGG - chr4_4:77598325-77598344 None:intergenic 30.0%
AACTGAGTTTATCTATGCAA+TGG + chr4_4:77598326-77598345 Msa0705070:exon 30.0%
TGTTACCTTCTCTAATGATT+CGG - chr4_4:77598505-77598524 None:intergenic 30.0%
TGCATCATTGAATTCTTGAT+TGG - chr4_4:77598538-77598557 None:intergenic 30.0%
!! ATCGGTTTTTAAGAAACTAC+GGG - chr4_4:77598652-77598671 None:intergenic 30.0%
!! GATCGGTTTTTAAGAAACTA+CGG - chr4_4:77598653-77598672 None:intergenic 30.0%
! TCAAAAGTGTACTTCTAAAC+GGG - chr4_4:77598675-77598694 None:intergenic 30.0%
!! GTCTAAAAGTGTACATTTTG+AGG + chr4_4:77598744-77598763 Msa0705070:intron 30.0%
!!! TGCATGATTGCATTTTTTCA+AGG - chr4_4:77598806-77598825 None:intergenic 30.0%
!! AGTGTACATATTTTGGATTG+TGG + chr4_4:77598929-77598948 Msa0705070:intron 30.0%
!! GTGTACATATTTTGGATTGT+GGG + chr4_4:77598930-77598949 Msa0705070:intron 30.0%
!! TGTACATATTTTGGATTGTG+GGG + chr4_4:77598931-77598950 Msa0705070:intron 30.0%
TAAGCGAATTAAAGTAGAAG+CGG + chr4_4:77598988-77599007 Msa0705070:CDS 30.0%
TCCTAACCTAAGGTAATAAA+AGG + chr4_4:77599105-77599124 Msa0705070:intron 30.0%
! TCCTTTTATTACCTTAGGTT+AGG - chr4_4:77599109-77599128 None:intergenic 30.0%
TTCAAACATCATAGTACGAT+AGG - chr4_4:77599222-77599241 None:intergenic 30.0%
! ATGATATTGGTCTACTCATT+TGG + chr4_4:77599296-77599315 Msa0705070:intron 30.0%
! TGATATTGGTCTACTCATTT+GGG + chr4_4:77599297-77599316 Msa0705070:intron 30.0%
AATACAATTGATGAGATGTG+TGG - chr4_4:77599642-77599661 None:intergenic 30.0%
AGTTAAACTTAACTAACCAG+TGG + chr4_4:77599832-77599851 Msa0705070:intron 30.0%
TACTACAAATGTTCAAGTGT+GGG + chr4_4:77600200-77600219 Msa0705070:CDS 30.0%
TGAGAGAATACAATTTGAGA+GGG - chr4_4:77600422-77600441 None:intergenic 30.0%
!!! TAAACTCAGTTTTGGGTTGT+TGG - chr4_4:77598317-77598336 None:intergenic 35.0%
GTAAACTCAGAAGAGGAATT+AGG - chr4_4:77598411-77598430 None:intergenic 35.0%
AGATAGTGTAAACTCAGAAG+AGG - chr4_4:77598418-77598437 None:intergenic 35.0%
ATCACCTTGATCAAGAATCT+CGG + chr4_4:77598469-77598488 Msa0705070:CDS 35.0%
!! AAGTACCGAATCATTAGAGA+AGG + chr4_4:77598497-77598516 Msa0705070:CDS 35.0%
GCAATACAAATTAAGGGAGA+AGG - chr4_4:77598588-77598607 None:intergenic 35.0%
!! TCGGTTTTTAAGAAACTACG+GGG - chr4_4:77598651-77598670 None:intergenic 35.0%
!! AAGTGTACATTTTGAGGGAT+CGG + chr4_4:77598750-77598769 Msa0705070:intron 35.0%
!! GGATCGGTTTTTAGAAATCA+TGG + chr4_4:77598766-77598785 Msa0705070:intron 35.0%
CAAATGAAGTAATGAACCGA+TGG - chr4_4:77598876-77598895 None:intergenic 35.0%
AAGCGAATTAAAGTAGAAGC+GGG + chr4_4:77598989-77599008 Msa0705070:CDS 35.0%
!! CTTGTTCTAAAGGCTATCTT+TGG - chr4_4:77599016-77599035 None:intergenic 35.0%
! AAGCTCTGATCTTGTTCTAA+AGG - chr4_4:77599026-77599045 None:intergenic 35.0%
AAGATCAGAGCTTGAGATTA+TGG + chr4_4:77599033-77599052 Msa0705070:CDS 35.0%
!! TGAACAAAGTAGCTTTAAGG+TGG + chr4_4:77599179-77599198 Msa0705070:intron 35.0%
! GATATTGGTCTACTCATTTG+GGG + chr4_4:77599298-77599317 Msa0705070:intron 35.0%
TTTAAGCATCAATCCATCGT+AGG + chr4_4:77599604-77599623 Msa0705070:intron 35.0%
TTAAGCATCAATCCATCGTA+GGG + chr4_4:77599605-77599624 Msa0705070:intron 35.0%
ACTTAACTAACCAGTGGATA+AGG + chr4_4:77599838-77599857 Msa0705070:intron 35.0%
TAGCTAGCTAGATAATGTTG+TGG + chr4_4:77599879-77599898 Msa0705070:intron 35.0%
CTACTACAAATGTTCAAGTG+TGG + chr4_4:77600199-77600218 Msa0705070:CDS 35.0%
TGGGATGTAATGTGAAGAAA+AGG + chr4_4:77600219-77600238 Msa0705070:CDS 35.0%
GGGATGTAATGTGAAGAAAA+GGG + chr4_4:77600220-77600239 Msa0705070:CDS 35.0%
ATGTAATGTGAAGAAAAGGG+TGG + chr4_4:77600223-77600242 Msa0705070:CDS 35.0%
CAGAATGCACAAAAGAGATT+TGG - chr4_4:77600334-77600353 None:intergenic 35.0%
AGTATGTATCATGTATGCAG+TGG - chr4_4:77600375-77600394 None:intergenic 35.0%
CAATTTGAGAGGGAGTTAAA+TGG - chr4_4:77600412-77600431 None:intergenic 35.0%
GTGAGAGAATACAATTTGAG+AGG - chr4_4:77600423-77600442 None:intergenic 35.0%
!!! TAAACTCAGTTTTGGGTTGT+TGG - chr4_4:77598317-77598336 None:intergenic 35.0%
GTAAACTCAGAAGAGGAATT+AGG - chr4_4:77598411-77598430 None:intergenic 35.0%
AGATAGTGTAAACTCAGAAG+AGG - chr4_4:77598418-77598437 None:intergenic 35.0%
ATCACCTTGATCAAGAATCT+CGG + chr4_4:77598469-77598488 Msa0705070:CDS 35.0%
!! AAGTACCGAATCATTAGAGA+AGG + chr4_4:77598497-77598516 Msa0705070:CDS 35.0%
GCAATACAAATTAAGGGAGA+AGG - chr4_4:77598588-77598607 None:intergenic 35.0%
!! TCGGTTTTTAAGAAACTACG+GGG - chr4_4:77598651-77598670 None:intergenic 35.0%
!! AAGTGTACATTTTGAGGGAT+CGG + chr4_4:77598750-77598769 Msa0705070:intron 35.0%
!! GGATCGGTTTTTAGAAATCA+TGG + chr4_4:77598766-77598785 Msa0705070:intron 35.0%
CAAATGAAGTAATGAACCGA+TGG - chr4_4:77598876-77598895 None:intergenic 35.0%
AAGCGAATTAAAGTAGAAGC+GGG + chr4_4:77598989-77599008 Msa0705070:CDS 35.0%
!! CTTGTTCTAAAGGCTATCTT+TGG - chr4_4:77599016-77599035 None:intergenic 35.0%
! AAGCTCTGATCTTGTTCTAA+AGG - chr4_4:77599026-77599045 None:intergenic 35.0%
AAGATCAGAGCTTGAGATTA+TGG + chr4_4:77599033-77599052 Msa0705070:CDS 35.0%
!! TGAACAAAGTAGCTTTAAGG+TGG + chr4_4:77599179-77599198 Msa0705070:intron 35.0%
! GATATTGGTCTACTCATTTG+GGG + chr4_4:77599298-77599317 Msa0705070:intron 35.0%
TTTAAGCATCAATCCATCGT+AGG + chr4_4:77599604-77599623 Msa0705070:intron 35.0%
TTAAGCATCAATCCATCGTA+GGG + chr4_4:77599605-77599624 Msa0705070:intron 35.0%
ACTTAACTAACCAGTGGATA+AGG + chr4_4:77599838-77599857 Msa0705070:intron 35.0%
TAGCTAGCTAGATAATGTTG+TGG + chr4_4:77599879-77599898 Msa0705070:intron 35.0%
CTACTACAAATGTTCAAGTG+TGG + chr4_4:77600199-77600218 Msa0705070:CDS 35.0%
TGGGATGTAATGTGAAGAAA+AGG + chr4_4:77600219-77600238 Msa0705070:CDS 35.0%
GGGATGTAATGTGAAGAAAA+GGG + chr4_4:77600220-77600239 Msa0705070:CDS 35.0%
ATGTAATGTGAAGAAAAGGG+TGG + chr4_4:77600223-77600242 Msa0705070:CDS 35.0%
CAGAATGCACAAAAGAGATT+TGG - chr4_4:77600334-77600353 None:intergenic 35.0%
AGTATGTATCATGTATGCAG+TGG - chr4_4:77600375-77600394 None:intergenic 35.0%
CAATTTGAGAGGGAGTTAAA+TGG - chr4_4:77600412-77600431 None:intergenic 35.0%
GTGAGAGAATACAATTTGAG+AGG - chr4_4:77600423-77600442 None:intergenic 35.0%
AGTAGAGTGAGCAAAGTTTG+GGG - chr4_4:77598379-77598398 None:intergenic 40.0%
GAGTAGAGTGAGCAAAGTTT+GGG - chr4_4:77598380-77598399 None:intergenic 40.0%
TGAGTAGAGTGAGCAAAGTT+TGG - chr4_4:77598381-77598400 None:intergenic 40.0%
AGTGTACTTCTAAACGGGAT+CGG - chr4_4:77598670-77598689 None:intergenic 40.0%
AGCGAATTAAAGTAGAAGCG+GGG + chr4_4:77598990-77599009 Msa0705070:CDS 40.0%
TTATGGACGATGGATACAAG+TGG + chr4_4:77599050-77599069 Msa0705070:CDS 40.0%
TGAAGAACAGTCCTAACCTA+AGG + chr4_4:77599095-77599114 Msa0705070:CDS 40.0%
! CTACTCATTTGGGGTTGAAT+CGG + chr4_4:77599307-77599326 Msa0705070:intron 40.0%
ACTAACCAGTGGATAAGGAT+AGG + chr4_4:77599843-77599862 Msa0705070:intron 40.0%
CTAACCAGTGGATAAGGATA+GGG + chr4_4:77599844-77599863 Msa0705070:intron 40.0%
CTAGCTAGATAATGTTGTGG+CGG + chr4_4:77599882-77599901 Msa0705070:intron 40.0%
CTGACTAATGGCTTGAGATA+GGG + chr4_4:77599917-77599936 Msa0705070:intron 40.0%
ATGTGAAGAAAAGGGTGGAA+AGG + chr4_4:77600228-77600247 Msa0705070:CDS 40.0%
TGTGAAGAAAAGGGTGGAAA+GGG + chr4_4:77600229-77600248 Msa0705070:CDS 40.0%
AAAAGAGATTTGGCTGCAGT+GGG - chr4_4:77600324-77600343 None:intergenic 40.0%
AGTAGAGTGAGCAAAGTTTG+GGG - chr4_4:77598379-77598398 None:intergenic 40.0%
GAGTAGAGTGAGCAAAGTTT+GGG - chr4_4:77598380-77598399 None:intergenic 40.0%
TGAGTAGAGTGAGCAAAGTT+TGG - chr4_4:77598381-77598400 None:intergenic 40.0%
AGTGTACTTCTAAACGGGAT+CGG - chr4_4:77598670-77598689 None:intergenic 40.0%
AGCGAATTAAAGTAGAAGCG+GGG + chr4_4:77598990-77599009 Msa0705070:CDS 40.0%
TTATGGACGATGGATACAAG+TGG + chr4_4:77599050-77599069 Msa0705070:CDS 40.0%
TGAAGAACAGTCCTAACCTA+AGG + chr4_4:77599095-77599114 Msa0705070:CDS 40.0%
! CTACTCATTTGGGGTTGAAT+CGG + chr4_4:77599307-77599326 Msa0705070:intron 40.0%
ACTAACCAGTGGATAAGGAT+AGG + chr4_4:77599843-77599862 Msa0705070:intron 40.0%
CTAACCAGTGGATAAGGATA+GGG + chr4_4:77599844-77599863 Msa0705070:intron 40.0%
CTAGCTAGATAATGTTGTGG+CGG + chr4_4:77599882-77599901 Msa0705070:intron 40.0%
CTGACTAATGGCTTGAGATA+GGG + chr4_4:77599917-77599936 Msa0705070:intron 40.0%
ATGTGAAGAAAAGGGTGGAA+AGG + chr4_4:77600228-77600247 Msa0705070:CDS 40.0%
TGTGAAGAAAAGGGTGGAAA+GGG + chr4_4:77600229-77600248 Msa0705070:CDS 40.0%
AAAAGAGATTTGGCTGCAGT+GGG - chr4_4:77600324-77600343 None:intergenic 40.0%
! CAAAGTTTGGGGTAGCATGA+GGG - chr4_4:77598368-77598387 None:intergenic 45.0%
GTGACCGAGATTCTTGATCA+AGG - chr4_4:77598476-77598495 None:intergenic 45.0%
GAGCTTGAGATTATGGACGA+TGG + chr4_4:77599040-77599059 Msa0705070:CDS 45.0%
GGATACAAGTGGAGGAAGTA+CGG + chr4_4:77599061-77599080 Msa0705070:CDS 45.0%
! GATACAAGTGGAGGAAGTAC+GGG + chr4_4:77599062-77599081 Msa0705070:CDS 45.0%
TTGATGAGATGTGTGGTGTC+AGG - chr4_4:77599635-77599654 None:intergenic 45.0%
TAAGGATAGGGCAGTACTTC+AGG + chr4_4:77599856-77599875 Msa0705070:intron 45.0%
!! CTGGTCATAGTGCTGACTAA+TGG + chr4_4:77599905-77599924 Msa0705070:intron 45.0%
GCTGACTAATGGCTTGAGAT+AGG + chr4_4:77599916-77599935 Msa0705070:intron 45.0%
GGGAGGTAAATGGAATCTCA+TGG - chr4_4:77600304-77600323 None:intergenic 45.0%
CAAAAGAGATTTGGCTGCAG+TGG - chr4_4:77600325-77600344 None:intergenic 45.0%
! CAAAGTTTGGGGTAGCATGA+GGG - chr4_4:77598368-77598387 None:intergenic 45.0%
GTGACCGAGATTCTTGATCA+AGG - chr4_4:77598476-77598495 None:intergenic 45.0%
GAGCTTGAGATTATGGACGA+TGG + chr4_4:77599040-77599059 Msa0705070:CDS 45.0%
GGATACAAGTGGAGGAAGTA+CGG + chr4_4:77599061-77599080 Msa0705070:CDS 45.0%
! GATACAAGTGGAGGAAGTAC+GGG + chr4_4:77599062-77599081 Msa0705070:CDS 45.0%
TTGATGAGATGTGTGGTGTC+AGG - chr4_4:77599635-77599654 None:intergenic 45.0%
TAAGGATAGGGCAGTACTTC+AGG + chr4_4:77599856-77599875 Msa0705070:intron 45.0%
!! CTGGTCATAGTGCTGACTAA+TGG + chr4_4:77599905-77599924 Msa0705070:intron 45.0%
GCTGACTAATGGCTTGAGAT+AGG + chr4_4:77599916-77599935 Msa0705070:intron 45.0%
GGGAGGTAAATGGAATCTCA+TGG - chr4_4:77600304-77600323 None:intergenic 45.0%
CAAAAGAGATTTGGCTGCAG+TGG - chr4_4:77600325-77600344 None:intergenic 45.0%
!!! ACATTTTATATTTTTTTTAA+AGG + chr4_4:77599732-77599751 Msa0705070:intron 5.0%
!!! ACATTTTATATTTTTTTTAA+AGG + chr4_4:77599732-77599751 Msa0705070:intron 5.0%
! GCAAAGTTTGGGGTAGCATG+AGG - chr4_4:77598369-77598388 None:intergenic 50.0%
TGGACGATGGATACAAGTGG+AGG + chr4_4:77599053-77599072 Msa0705070:CDS 50.0%
ACTGCCCTATCCTTATCCAC+TGG - chr4_4:77599851-77599870 None:intergenic 50.0%
CTAGATAATGTTGTGGCGGC+TGG + chr4_4:77599886-77599905 Msa0705070:intron 50.0%
! GCAAAGTTTGGGGTAGCATG+AGG - chr4_4:77598369-77598388 None:intergenic 50.0%
TGGACGATGGATACAAGTGG+AGG + chr4_4:77599053-77599072 Msa0705070:CDS 50.0%
ACTGCCCTATCCTTATCCAC+TGG - chr4_4:77599851-77599870 None:intergenic 50.0%
CTAGATAATGTTGTGGCGGC+TGG + chr4_4:77599886-77599905 Msa0705070:intron 50.0%
TGGCTGCAGTGGGAGGTAAA+TGG - chr4_4:77600314-77600333 None:intergenic 55.0%
AGAGATTTGGCTGCAGTGGG+AGG - chr4_4:77600321-77600340 None:intergenic 55.0%
TGGCTGCAGTGGGAGGTAAA+TGG - chr4_4:77600314-77600333 None:intergenic 55.0%
AGAGATTTGGCTGCAGTGGG+AGG - chr4_4:77600321-77600340 None:intergenic 55.0%
GTGTCAGGTGCTCCCTACGA+TGG - chr4_4:77599620-77599639 None:intergenic 60.0%
GTGTCAGGTGCTCCCTACGA+TGG - chr4_4:77599620-77599639 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 77598278 77600468 77598278 ID=Msa0705070;Name=Msa0705070
chr4_4 mRNA 77598278 77600468 77598278 ID=Msa0705070-mRNA-1;Parent=Msa0705070;Name=Msa0705070-mRNA-1;_AED=0.13;_eAED=0.13;_QI=67|1|1|1|1|1|3|101|183
chr4_4 exon 77598278 77598577 77598278 ID=Msa0705070-mRNA-1:exon:24671;Parent=Msa0705070-mRNA-1
chr4_4 exon 77598970 77599116 77598970 ID=Msa0705070-mRNA-1:exon:24672;Parent=Msa0705070-mRNA-1
chr4_4 exon 77600196 77600468 77600196 ID=Msa0705070-mRNA-1:exon:24673;Parent=Msa0705070-mRNA-1
chr4_4 five_prime_UTR 77598278 77598344 77598278 ID=Msa0705070-mRNA-1:five_prime_utr;Parent=Msa0705070-mRNA-1
chr4_4 CDS 77598345 77598577 77598345 ID=Msa0705070-mRNA-1:cds;Parent=Msa0705070-mRNA-1
chr4_4 CDS 77598970 77599116 77598970 ID=Msa0705070-mRNA-1:cds;Parent=Msa0705070-mRNA-1
chr4_4 CDS 77600196 77600367 77600196 ID=Msa0705070-mRNA-1:cds;Parent=Msa0705070-mRNA-1
chr4_4 three_prime_UTR 77600368 77600468 77600368 ID=Msa0705070-mRNA-1:three_prime_utr;Parent=Msa0705070-mRNA-1
Gene Sequence

>Msa0705070

ATGGACTACTATTTTGTAAACCCTCATGCTACCCCAAACTTTGCTCACTCTACTCATATGATTCCTAATTCCTCTTCTGAGTTTACACTATCTGATTATCTTATGCTTGATGATATTTGTATTGATCACCTTGATCAAGAATCTCGGTCACAAAGTACCGAATCATTAGAGAAGGTAACATTCAATGATGCCAATCAAGAATTCAATGATGCAACCTCAAAGAACAATAATATAAAATACAAAAATGGGATTAAGCGAATTAAAGTAGAAGCGGGGCCAAAGATAGCCTTTAGAACAAGATCAGAGCTTGAGATTATGGACGATGGATACAAGTGGAGGAAGTACGGGAAGAAGTCTGTGAAGAACAGTCCTAACCTAAGGAACTACTACAAATGTTCAAGTGTGGGATGTAATGTGAAGAAAAGGGTGGAAAGGGATAGAGATGATTCGAGCTATGTGATAACAAGTTATGAAGGTGTGCATAACCATGAGATTCCATTTACCTCCCACTGCAGCCAAATCTCTTTTGTGCATTCTGATGAATATAATTAA
Protein sequence

>Msa0705070

MDYYFVNPHATPNFAHSTHMIPNSSSEFTLSDYLMLDDICIDHLDQESRSQSTESLEKVTFNDANQEFNDATSKNNNIKYKNGIKRIKVEAGPKIAFRTRSELEIMDDGYKWRKYGKKSVKNSPNLRNYYKCSSVGCNVKKRVERDRDDSSYVITSYEGVHNHEIPFTSHCSQISFVHSDEYN*