AlfalfaGEDB | Msa0705220

M. sativa cultivar zhongmu-4 / Msa0705220

KEGG: -
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705220	AET04681.1	91.429	35	3	0	45	79	154	188	1.78e-12	69.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705220	G7LD42	91.429	35	3	0	45	79	154	188	8.52e-13	69.7

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0705220	AT2G31880.1	63.415	41	15	0	1	41	479	519	8.05e-11	56.6

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Find 13 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACATCTCTGCTATAGATAAT+TGG	0.262154	4_4:+77767898	None:intergenic
AGACTTACAATAACAATGTT+TGG	0.366900	4_4:-77768255	Msa0705220:CDS
AGCAGAGATGTACCAAATAA+GGG	0.490549	4_4:-77767886	Msa0705220:CDS
GATACAAAGGCTACTTACCT+GGG	0.612670	4_4:+77767841	None:intergenic
GATGAATTATGCGCGAATTA+TGG	0.257687	4_4:+77768340	None:intergenic
GATGTACCAAATAAGGGAGC+AGG	0.536621	4_4:-77767880	Msa0705220:CDS
GCTTTGATCGATATTCACCC+AGG	0.642140	4_4:-77767858	Msa0705220:CDS
TAGCAGAGATGTACCAAATA+AGG	0.268582	4_4:-77767887	Msa0705220:CDS
TATTCTACTTGATAATGATA+TGG	0.356664	4_4:-77768298	Msa0705220:CDS
TCAAAGCCTGCTCCCTTATT+TGG	0.163694	4_4:+77767874	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACATCTCTGCTATAGATAAT+TGG	+	chr4_4:77768305-77768324	None:intergenic	30.0%
GATGAATTATGCGCGAATTA+TGG	+	chr4_4:77767863-77767882	None:intergenic	35.0%
CATATATCCCAATATTGCAG+AGG	+	chr4_4:77768000-77768019	None:intergenic	35.0%
CTCTGCAATATTGGGATATA+TGG	-	chr4_4:77767998-77768017	Msa0705220:intron	35.0%
TAGCAGAGATGTACCAAATA+AGG	-	chr4_4:77768313-77768332	Msa0705220:CDS	35.0%
AGCAGAGATGTACCAAATAA+GGG	-	chr4_4:77768314-77768333	Msa0705220:CDS	35.0%
GCGCCATAGTATAACCAATA+CGG	-	chr4_4:77768020-77768039	Msa0705220:intron	40.0%
TAGTATAACCAATACGGACG+CGG	-	chr4_4:77768026-77768045	Msa0705220:intron	40.0%
GATGAAGTCAGGAAATCTCA+AGG	-	chr4_4:77768176-77768195	Msa0705220:intron	40.0%
TCAAGGATGCAATTGATGCA+AGG	-	chr4_4:77768193-77768212	Msa0705220:intron	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_4	gene	77767855	77768367	77767855	ID=Msa0705220;Name=Msa0705220
chr4_4	mRNA	77767855	77768367	77767855	ID=Msa0705220-mRNA-1;Parent=Msa0705220;Name=Msa0705220-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0\|0\|0\|1\|0\|0\|2\|0\|79
chr4_4	exon	77768236	77768367	77768236	ID=Msa0705220-mRNA-1:exon:24762;Parent=Msa0705220-mRNA-1
chr4_4	exon	77767855	77767962	77767855	ID=Msa0705220-mRNA-1:exon:24761;Parent=Msa0705220-mRNA-1
chr4_4	CDS	77768236	77768367	77768236	ID=Msa0705220-mRNA-1:cds;Parent=Msa0705220-mRNA-1
chr4_4	CDS	77767855	77767962	77767855	ID=Msa0705220-mRNA-1:cds;Parent=Msa0705220-mRNA-1

Gene Sequence	>Msa0705220 ATGAGCCATAATTCGCGCATAATTCATCGAGATCTCAAGCCTACTAATATTCTACTTGATAATGATATGGAAGCTAAGATTACAAATTTTAGACTTACAATAACAATGTTTGGTGCGCAGACTCGTAGAAGTGGTACTTCCTTGAATACATTTGACCTAAAAGGAAAATTGTATCCAATTATCTATAGCAGAGATGTACCAAATAAGGGAGCAGGCTTTGATCGATATTCACCCAGGTAA
Protein sequence	>Msa0705220 MSHNSRIIHRDLKPTNILLDNDMEAKITNFRLTITMFGAQTRRSGTSLNTFDLKGKLYPIIYSRDVPNKGAGFDRYSPR*