Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0705220 | AET04681.1 | 91.429 | 35 | 3 | 0 | 45 | 79 | 154 | 188 | 1.78e-12 | 69.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0705220 | G7LD42 | 91.429 | 35 | 3 | 0 | 45 | 79 | 154 | 188 | 8.52e-13 | 69.7 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0705220 | AT2G31880.1 | 63.415 | 41 | 15 | 0 | 1 | 41 | 479 | 519 | 8.05e-11 | 56.6 |
Find 13 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAAAGCCTGCTCCCTTATT+TGG | 0.163694 | 4_4:+77767874 | None:intergenic |
| TTGAATACATTTGACCTAAA+AGG | 0.209512 | 4_4:-77767931 | Msa0705220:CDS |
| GATGAATTATGCGCGAATTA+TGG | 0.257687 | 4_4:+77768340 | None:intergenic |
| ACATCTCTGCTATAGATAAT+TGG | 0.262154 | 4_4:+77767898 | None:intergenic |
| TAGCAGAGATGTACCAAATA+AGG | 0.268582 | 4_4:-77767887 | Msa0705220:CDS |
| TATTCTACTTGATAATGATA+TGG | 0.356664 | 4_4:-77768298 | Msa0705220:CDS |
| AGACTTACAATAACAATGTT+TGG | 0.366900 | 4_4:-77768255 | Msa0705220:CDS |
| TGATACAAAGGCTACTTACC+TGG | 0.457752 | 4_4:+77767840 | None:intergenic |
| TTATCAAGTAGAATATTAGT+AGG | 0.475986 | 4_4:+77768306 | None:intergenic |
| AGCAGAGATGTACCAAATAA+GGG | 0.490549 | 4_4:-77767886 | Msa0705220:CDS |
| GATGTACCAAATAAGGGAGC+AGG | 0.536621 | 4_4:-77767880 | Msa0705220:CDS |
| GATACAAAGGCTACTTACCT+GGG | 0.612670 | 4_4:+77767841 | None:intergenic |
| GCTTTGATCGATATTCACCC+AGG | 0.642140 | 4_4:-77767858 | Msa0705220:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATCAAGTAGAATATTAGT+AGG | + | chr4_4:77767897-77767916 | None:intergenic | 20.0% |
| !!! | TATTCTACTTGATAATGATA+TGG | - | chr4_4:77767902-77767921 | Msa0705220:CDS | 20.0% |
| !! | TCCATGAAAAAATCATTATT+GGG | + | chr4_4:77768123-77768142 | None:intergenic | 20.0% |
| !! | TGAAGCAAAAATATTAATGT+TGG | + | chr4_4:77768233-77768252 | None:intergenic | 20.0% |
| !!! | AATATTTTTGCTTCATGTTT+AGG | - | chr4_4:77768238-77768257 | Msa0705220:CDS | 20.0% |
| !!! | ATATTTTTGCTTCATGTTTA+GGG | - | chr4_4:77768239-77768258 | Msa0705220:CDS | 20.0% |
| ! | AGACTTACAATAACAATGTT+TGG | - | chr4_4:77767945-77767964 | Msa0705220:intron | 25.0% |
| !! | AAGAGTGACATATATAGTTT+TGG | - | chr4_4:77768063-77768082 | Msa0705220:intron | 25.0% |
| !!! | TATAGTTTTGGTGTTATTCT+TGG | - | chr4_4:77768075-77768094 | Msa0705220:intron | 25.0% |
| !!! | TCCCAATAATGATTTTTTCA+TGG | - | chr4_4:77768119-77768138 | Msa0705220:intron | 25.0% |
| ! | GTCCATGAAAAAATCATTAT+TGG | + | chr4_4:77768124-77768143 | None:intergenic | 25.0% |
| !!! | ATAATGATTTTTTCATGGAC+CGG | - | chr4_4:77768124-77768143 | Msa0705220:intron | 25.0% |
| ! | ATTAACAATTTGATGAAGTC+AGG | - | chr4_4:77768165-77768184 | Msa0705220:intron | 25.0% |
| !!! | TTTTAGGTCAAATGTATTCA+AGG | + | chr4_4:77768270-77768289 | None:intergenic | 25.0% |
| ! | TTGAATACATTTGACCTAAA+AGG | - | chr4_4:77768269-77768288 | Msa0705220:CDS | 25.0% |
| !! | TTGGATACAATTTTCCTTTT+AGG | + | chr4_4:77768286-77768305 | None:intergenic | 25.0% |
| !!! | TAATGATTTTTTCATGGACC+GGG | - | chr4_4:77768125-77768144 | Msa0705220:intron | 30.0% |
| ACATCTCTGCTATAGATAAT+TGG | + | chr4_4:77768305-77768324 | None:intergenic | 30.0% | |
| GATGAATTATGCGCGAATTA+TGG | + | chr4_4:77767863-77767882 | None:intergenic | 35.0% | |
| CATATATCCCAATATTGCAG+AGG | + | chr4_4:77768000-77768019 | None:intergenic | 35.0% | |
| CTCTGCAATATTGGGATATA+TGG | - | chr4_4:77767998-77768017 | Msa0705220:intron | 35.0% | |
| !!! | TGGTGTTATTCTTGGTGTTT+TGG | - | chr4_4:77768083-77768102 | Msa0705220:intron | 35.0% |
| !!! | ATTCTTGGTGTTTTGGTAAG+TGG | - | chr4_4:77768090-77768109 | Msa0705220:intron | 35.0% |
| !!! | AATGATTTTTTCATGGACCG+GGG | - | chr4_4:77768126-77768145 | Msa0705220:intron | 35.0% |
| TAGCAGAGATGTACCAAATA+AGG | - | chr4_4:77768313-77768332 | Msa0705220:CDS | 35.0% | |
| AGCAGAGATGTACCAAATAA+GGG | - | chr4_4:77768314-77768333 | Msa0705220:CDS | 35.0% | |
| GCGCCATAGTATAACCAATA+CGG | - | chr4_4:77768020-77768039 | Msa0705220:intron | 40.0% | |
| !! | CGTCCGTATTGGTTATACTA+TGG | + | chr4_4:77768026-77768045 | None:intergenic | 40.0% |
| TAGTATAACCAATACGGACG+CGG | - | chr4_4:77768026-77768045 | Msa0705220:intron | 40.0% | |
| GATGAAGTCAGGAAATCTCA+AGG | - | chr4_4:77768176-77768195 | Msa0705220:intron | 40.0% | |
| TCAAGGATGCAATTGATGCA+AGG | - | chr4_4:77768193-77768212 | Msa0705220:intron | 40.0% | |
| TGATCACGCCTCTGCAATAT+TGG | - | chr4_4:77767989-77768008 | Msa0705220:intron | 45.0% | |
| GATCACGCCTCTGCAATATT+GGG | - | chr4_4:77767990-77768009 | Msa0705220:intron | 45.0% | |
| TCCATTTGTCCAAACATCCC+CGG | + | chr4_4:77768146-77768165 | None:intergenic | 45.0% | |
| GATGTACCAAATAAGGGAGC+AGG | - | chr4_4:77768320-77768339 | Msa0705220:CDS | 45.0% | |
| TCAAAGCCTGCTCCCTTATT+TGG | + | chr4_4:77768329-77768348 | None:intergenic | 45.0% | |
| GCTTTGATCGATATTCACCC+AGG | - | chr4_4:77768342-77768361 | Msa0705220:CDS | 45.0% | |
| TATCACTACCGCGTCCGTAT+TGG | + | chr4_4:77768037-77768056 | None:intergenic | 50.0% | |
| TTTCATGGACCGGGGATGTT+TGG | - | chr4_4:77768134-77768153 | Msa0705220:intron | 50.0% | |
| ACCGGGGATGTTTGGACAAA+TGG | - | chr4_4:77768142-77768161 | Msa0705220:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 77767855 | 77768367 | 77767855 | ID=Msa0705220;Name=Msa0705220 |
| chr4_4 | mRNA | 77767855 | 77768367 | 77767855 | ID=Msa0705220-mRNA-1;Parent=Msa0705220;Name=Msa0705220-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|0|0|2|0|79 |
| chr4_4 | exon | 77768236 | 77768367 | 77768236 | ID=Msa0705220-mRNA-1:exon:24762;Parent=Msa0705220-mRNA-1 |
| chr4_4 | exon | 77767855 | 77767962 | 77767855 | ID=Msa0705220-mRNA-1:exon:24761;Parent=Msa0705220-mRNA-1 |
| chr4_4 | CDS | 77768236 | 77768367 | 77768236 | ID=Msa0705220-mRNA-1:cds;Parent=Msa0705220-mRNA-1 |
| chr4_4 | CDS | 77767855 | 77767962 | 77767855 | ID=Msa0705220-mRNA-1:cds;Parent=Msa0705220-mRNA-1 |
| Gene Sequence |
| Protein sequence |
