Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0706650 | XP_003630555.2 | 99.444 | 180 | 1 | 0 | 1 | 180 | 1 | 180 | 1.83e-126 | 368 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0706650 | sp|Q8S8C6|BPC4_ARATH | 60.684 | 117 | 43 | 2 | 1 | 116 | 1 | 115 | 1.62e-38 | 136 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0706650 | G7LI34 | 99.444 | 180 | 1 | 0 | 1 | 180 | 1 | 180 | 8.73e-127 | 368 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0706650 | TF | BBR-BPC |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0706650 | MtrunA17_Chr8g0385961 | 99.444 | 180 | 1 | 0 | 1 | 180 | 1 | 180 | 1.68e-130 | 368 |
| Msa0706650 | MtrunA17_Chr1g0156031 | 44.920 | 187 | 83 | 9 | 3 | 179 | 2 | 178 | 3.93e-32 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0706650 | AT2G21240.1 | 60.684 | 117 | 43 | 2 | 1 | 116 | 1 | 115 | 1.65e-39 | 136 |
| Msa0706650 | AT2G21240.2 | 60.684 | 117 | 43 | 2 | 1 | 116 | 1 | 115 | 1.65e-39 | 136 |
| Msa0706650 | AT4G38910.2 | 42.442 | 172 | 79 | 4 | 1 | 172 | 1 | 152 | 7.42e-33 | 119 |
| Msa0706650 | AT4G38910.1 | 42.553 | 141 | 63 | 3 | 33 | 172 | 4 | 127 | 1.87e-22 | 90.9 |
| Msa0706650 | AT5G42520.1 | 37.297 | 185 | 85 | 5 | 1 | 169 | 1 | 170 | 4.27e-19 | 83.2 |
| Msa0706650 | AT5G42520.2 | 37.838 | 185 | 80 | 5 | 1 | 169 | 1 | 166 | 4.37e-19 | 83.2 |
Find 46 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTGTCATGTTGGATATA+TGG | 0.216505 | 4_4:-79662404 | None:intergenic |
| TCAGAAGTTATAACAGTAAC+TGG | 0.272157 | 4_4:-79662468 | None:intergenic |
| TTTGTTCCTTTATTTGATGC+TGG | 0.301136 | 4_4:-79662101 | None:intergenic |
| TTGTTCCTTTATTTGATGCT+GGG | 0.361945 | 4_4:-79662100 | None:intergenic |
| CGTTGAATTCCACCATTGAA+TGG | 0.413813 | 4_4:-79662351 | None:intergenic |
| CAGTACTATTCCGACTTTGA+AGG | 0.424329 | 4_4:-79662320 | None:intergenic |
| TGCCTGCCTTTCAGCCATAA+TGG | 0.435049 | 4_4:-79662154 | None:intergenic |
| GGATCCCCAGCATCAAATAA+AGG | 0.448615 | 4_4:+79662095 | Msa0706650:CDS |
| GGATAATGCTCTACTGGAAC+GGG | 0.450868 | 4_4:+79662281 | Msa0706650:CDS |
| ACTGGCAACTTTCCATTCAA+TGG | 0.459914 | 4_4:+79662339 | Msa0706650:CDS |
| ATCTGAAAAGAATGAAGCCC+TGG | 0.461756 | 4_4:+79662209 | Msa0706650:CDS |
| GTGCTTCATCCCTCTGCCGA+AGG | 0.475401 | 4_4:-79662248 | None:intergenic |
| GTCCATTATGGCTGAAAGGC+AGG | 0.476045 | 4_4:+79662152 | Msa0706650:CDS |
| TGGATATATGGTTTGAAGAA+TGG | 0.483617 | 4_4:-79662392 | None:intergenic |
| TGAAGAATGGTGCATCCGCT+TGG | 0.493558 | 4_4:-79662379 | None:intergenic |
| TGAAAACGGTAGGCATAAGA+TGG | 0.514492 | 4_4:+79661943 | Msa0706650:CDS |
| TCAGAGGGATAATGCTCTAC+TGG | 0.518249 | 4_4:+79662275 | Msa0706650:CDS |
| GGGCTACGTCTCGAGCAGCC+AGG | 0.518686 | 4_4:-79662227 | None:intergenic |
| CTTCAAAGTCGGAATAGTAC+TGG | 0.519466 | 4_4:+79662321 | Msa0706650:CDS |
| TGTTCCTTTATTTGATGCTG+GGG | 0.520374 | 4_4:-79662099 | None:intergenic |
| AGTACTATTCCGACTTTGAA+GGG | 0.520694 | 4_4:-79662319 | None:intergenic |
| GGATGAAGCACTTGCTCAGA+GGG | 0.522036 | 4_4:+79662260 | Msa0706650:CDS |
| ATATGCTGCTTCTGTCATGT+TGG | 0.532878 | 4_4:-79662412 | None:intergenic |
| GAGACGTAGCCCTTCGGCAG+AGG | 0.546456 | 4_4:+79662238 | Msa0706650:CDS |
| GGCTACGTCTCGAGCAGCCA+GGG | 0.552588 | 4_4:-79662226 | None:intergenic |
| CAGAAGTTATAACAGTAACT+GGG | 0.557616 | 4_4:-79662467 | None:intergenic |
| TTTAAATGTGCAGTGGAATG+TGG | 0.558628 | 4_4:+79662074 | Msa0706650:intron |
| CTGCTCGAGACGTAGCCCTT+CGG | 0.563490 | 4_4:+79662232 | Msa0706650:CDS |
| CTACAGAGGAGCACATTCCC+TGG | 0.565304 | 4_4:+79661970 | Msa0706650:CDS |
| AGACGTAGCCCTTCGGCAGA+GGG | 0.565616 | 4_4:+79662239 | Msa0706650:CDS |
| CTCTTGCAGCCCTTCAAAGT+CGG | 0.574408 | 4_4:+79662310 | Msa0706650:CDS |
| TGCTTCATCCCTCTGCCGAA+GGG | 0.577861 | 4_4:-79662247 | None:intergenic |
| GGGATAATGCTCTACTGGAA+CGG | 0.583003 | 4_4:+79662280 | Msa0706650:CDS |
| TCTTCTTGAACTTGAACTAG+AGG | 0.590105 | 4_4:+79662179 | Msa0706650:CDS |
| TTAGGTCCATTATGGCTGAA+AGG | 0.590519 | 4_4:+79662148 | Msa0706650:CDS |
| GATGACCGCCAATATGAAAA+CGG | 0.592001 | 4_4:+79661929 | Msa0706650:CDS |
| GGCAACTTTCCATTCAATGG+TGG | 0.607937 | 4_4:+79662342 | Msa0706650:CDS |
| CATAAGATGGACTACTACAG+AGG | 0.628336 | 4_4:+79661956 | Msa0706650:CDS |
| TCATCTAACTAAGAGAACAA+AGG | 0.650253 | 4_4:+79662503 | Msa0706650:CDS |
| ACCGCCAATATGAAAACGGT+AGG | 0.665966 | 4_4:+79661933 | Msa0706650:CDS |
| GGGATGAAGCACTTGCTCAG+AGG | 0.669930 | 4_4:+79662259 | Msa0706650:CDS |
| AGTTATAACAGTAACTGGGG+AGG | 0.670600 | 4_4:-79662463 | None:intergenic |
| AGAAGTTATAACAGTAACTG+GGG | 0.682187 | 4_4:-79662466 | None:intergenic |
| GAATTCAACGTGGATCCAAG+CGG | 0.708406 | 4_4:+79662364 | Msa0706650:CDS |
| TTCAATGGTGGAATTCAACG+TGG | 0.712678 | 4_4:+79662354 | Msa0706650:CDS |
| AGAAGCAGCATATAGCACGA+CGG | 0.752304 | 4_4:+79662422 | Msa0706650:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTTTTATTCATAACTA+GGG | - | chr4_4:79662130-79662149 | None:intergenic | 10.0% |
| !!! | TAATTTTTTTATTCATAACT+AGG | - | chr4_4:79662131-79662150 | None:intergenic | 10.0% |
| !! | TAGTTATGAATAAAAAAATT+AGG | + | chr4_4:79662130-79662149 | Msa0706650:CDS | 10.0% |
| !! | TAAAAAAATTAGGTCCATTA+TGG | + | chr4_4:79662140-79662159 | Msa0706650:CDS | 20.0% |
| !! | ATTGGGTTGATTTATTGGAT+TGG | + | chr4_4:79662049-79662068 | Msa0706650:intron | 30.0% |
| ! | ATTGGATTTTAAATGTGCAG+TGG | + | chr4_4:79662067-79662086 | Msa0706650:intron | 30.0% |
| ! | TTGTTCCTTTATTTGATGCT+GGG | - | chr4_4:79662103-79662122 | None:intergenic | 30.0% |
| ! | TTTGTTCCTTTATTTGATGC+TGG | - | chr4_4:79662104-79662123 | None:intergenic | 30.0% |
| TGGATATATGGTTTGAAGAA+TGG | - | chr4_4:79662395-79662414 | None:intergenic | 30.0% | |
| AGAAGTTATAACAGTAACTG+GGG | - | chr4_4:79662469-79662488 | None:intergenic | 30.0% | |
| CAGAAGTTATAACAGTAACT+GGG | - | chr4_4:79662470-79662489 | None:intergenic | 30.0% | |
| TCAGAAGTTATAACAGTAAC+TGG | - | chr4_4:79662471-79662490 | None:intergenic | 30.0% | |
| TCATCTAACTAAGAGAACAA+AGG | + | chr4_4:79662503-79662522 | Msa0706650:CDS | 30.0% | |
| ! | TATGCCTACCGTTTTCATAT+TGG | - | chr4_4:79661940-79661959 | None:intergenic | 35.0% |
| TATGTGCATTCTACTTACCA+GGG | - | chr4_4:79661990-79662009 | None:intergenic | 35.0% | |
| ATATGTGCATTCTACTTACC+AGG | - | chr4_4:79661991-79662010 | None:intergenic | 35.0% | |
| !! | TTAACTTGCAGTTGCACTAT+TGG | + | chr4_4:79662031-79662050 | Msa0706650:intron | 35.0% |
| ! | TAACTTGCAGTTGCACTATT+GGG | + | chr4_4:79662032-79662051 | Msa0706650:intron | 35.0% |
| !! | GCACTATTGGGTTGATTTAT+TGG | + | chr4_4:79662044-79662063 | Msa0706650:intron | 35.0% |
| TTTAAATGTGCAGTGGAATG+TGG | + | chr4_4:79662074-79662093 | Msa0706650:intron | 35.0% | |
| ! | TGTTCCTTTATTTGATGCTG+GGG | - | chr4_4:79662102-79662121 | None:intergenic | 35.0% |
| TCTTCTTGAACTTGAACTAG+AGG | + | chr4_4:79662179-79662198 | Msa0706650:CDS | 35.0% | |
| AGTACTATTCCGACTTTGAA+GGG | - | chr4_4:79662322-79662341 | None:intergenic | 35.0% | |
| CTTCTGTCATGTTGGATATA+TGG | - | chr4_4:79662407-79662426 | None:intergenic | 35.0% | |
| GATGACCGCCAATATGAAAA+CGG | + | chr4_4:79661929-79661948 | Msa0706650:CDS | 40.0% | |
| TGAAAACGGTAGGCATAAGA+TGG | + | chr4_4:79661943-79661962 | Msa0706650:CDS | 40.0% | |
| !! | CATAAGATGGACTACTACAG+AGG | + | chr4_4:79661956-79661975 | Msa0706650:CDS | 40.0% |
| TTAGGTCCATTATGGCTGAA+AGG | + | chr4_4:79662148-79662167 | Msa0706650:CDS | 40.0% | |
| ATCTGAAAAGAATGAAGCCC+TGG | + | chr4_4:79662209-79662228 | Msa0706650:CDS | 40.0% | |
| CAGTACTATTCCGACTTTGA+AGG | - | chr4_4:79662323-79662342 | None:intergenic | 40.0% | |
| CTTCAAAGTCGGAATAGTAC+TGG | + | chr4_4:79662321-79662340 | Msa0706650:CDS | 40.0% | |
| ACTGGCAACTTTCCATTCAA+TGG | + | chr4_4:79662339-79662358 | Msa0706650:CDS | 40.0% | |
| CGTTGAATTCCACCATTGAA+TGG | - | chr4_4:79662354-79662373 | None:intergenic | 40.0% | |
| ! | TTCAATGGTGGAATTCAACG+TGG | + | chr4_4:79662354-79662373 | Msa0706650:CDS | 40.0% |
| ! | ATATGCTGCTTCTGTCATGT+TGG | - | chr4_4:79662415-79662434 | None:intergenic | 40.0% |
| AGTTATAACAGTAACTGGGG+AGG | - | chr4_4:79662466-79662485 | None:intergenic | 40.0% | |
| ACCGCCAATATGAAAACGGT+AGG | + | chr4_4:79661933-79661952 | Msa0706650:CDS | 45.0% | |
| ! | GCCTACCGTTTTCATATTGG+CGG | - | chr4_4:79661937-79661956 | None:intergenic | 45.0% |
| GGATCCCCAGCATCAAATAA+AGG | + | chr4_4:79662095-79662114 | Msa0706650:CDS | 45.0% | |
| TCAGAGGGATAATGCTCTAC+TGG | + | chr4_4:79662275-79662294 | Msa0706650:CDS | 45.0% | |
| GGGATAATGCTCTACTGGAA+CGG | + | chr4_4:79662280-79662299 | Msa0706650:CDS | 45.0% | |
| GGATAATGCTCTACTGGAAC+GGG | + | chr4_4:79662281-79662300 | Msa0706650:CDS | 45.0% | |
| GGCAACTTTCCATTCAATGG+TGG | + | chr4_4:79662342-79662361 | Msa0706650:CDS | 45.0% | |
| GAATTCAACGTGGATCCAAG+CGG | + | chr4_4:79662364-79662383 | Msa0706650:CDS | 45.0% | |
| AGAAGCAGCATATAGCACGA+CGG | + | chr4_4:79662422-79662441 | Msa0706650:CDS | 45.0% | |
| GTCCATTATGGCTGAAAGGC+AGG | + | chr4_4:79662152-79662171 | Msa0706650:CDS | 50.0% | |
| TGCCTGCCTTTCAGCCATAA+TGG | - | chr4_4:79662157-79662176 | None:intergenic | 50.0% | |
| ! | GGATGAAGCACTTGCTCAGA+GGG | + | chr4_4:79662260-79662279 | Msa0706650:CDS | 50.0% |
| CTCTTGCAGCCCTTCAAAGT+CGG | + | chr4_4:79662310-79662329 | Msa0706650:CDS | 50.0% | |
| TGAAGAATGGTGCATCCGCT+TGG | - | chr4_4:79662382-79662401 | None:intergenic | 50.0% | |
| ! | CTACAGAGGAGCACATTCCC+TGG | + | chr4_4:79661970-79661989 | Msa0706650:CDS | 55.0% |
| TGCTTCATCCCTCTGCCGAA+GGG | - | chr4_4:79662250-79662269 | None:intergenic | 55.0% | |
| ! | GGGATGAAGCACTTGCTCAG+AGG | + | chr4_4:79662259-79662278 | Msa0706650:CDS | 55.0% |
| CTGCTCGAGACGTAGCCCTT+CGG | + | chr4_4:79662232-79662251 | Msa0706650:CDS | 60.0% | |
| AGACGTAGCCCTTCGGCAGA+GGG | + | chr4_4:79662239-79662258 | Msa0706650:CDS | 60.0% | |
| GTGCTTCATCCCTCTGCCGA+AGG | - | chr4_4:79662251-79662270 | None:intergenic | 60.0% | |
| GGCTACGTCTCGAGCAGCCA+GGG | - | chr4_4:79662229-79662248 | None:intergenic | 65.0% | |
| GAGACGTAGCCCTTCGGCAG+AGG | + | chr4_4:79662238-79662257 | Msa0706650:CDS | 65.0% | |
| GGGCTACGTCTCGAGCAGCC+AGG | - | chr4_4:79662230-79662249 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 79661923 | 79662560 | 79661923 | ID=Msa0706650;Name=Msa0706650 |
| chr4_4 | mRNA | 79661923 | 79662560 | 79661923 | ID=Msa0706650-mRNA-1;Parent=Msa0706650;Name=Msa0706650-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|0|0|1|1|1|2|0|180 |
| chr4_4 | exon | 79661923 | 79661991 | 79661923 | ID=Msa0706650-mRNA-1:exon:25670;Parent=Msa0706650-mRNA-1 |
| chr4_4 | exon | 79662087 | 79662560 | 79662087 | ID=Msa0706650-mRNA-1:exon:25671;Parent=Msa0706650-mRNA-1 |
| chr4_4 | CDS | 79661923 | 79661991 | 79661923 | ID=Msa0706650-mRNA-1:cds;Parent=Msa0706650-mRNA-1 |
| chr4_4 | CDS | 79662087 | 79662560 | 79662087 | ID=Msa0706650-mRNA-1:cds;Parent=Msa0706650-mRNA-1 |
| Gene Sequence |
| Protein sequence |
