AlfalfaGEDB | Msa0710840

M. sativa cultivar zhongmu-4 / Msa0710840

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0710840	AES95290.1	73.460	211	26	3	1	201	1	191	1.75e-98	293

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0710840	G7K023	73.460	211	26	3	1	201	1	191	8.36e-99	293

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TFs/TRs:

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Gene ID	Type	Classification
Msa0710840	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0710840	MtrunA17_Chr5g0406781	55.975	159	30	4	1	159	1	119	4.33e-50	158

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 68 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAACTTTCAAGGGTAGT+TGG	0.386873	5_1:+2653793	None:intergenic
AACCGCTCTCGAAGTCTTAA+TGG	0.238654	5_1:-2653743	Msa0710840:CDS
AAGAACTTTCAAGGGTAGTT+GGG	0.430510	5_1:+2653794	None:intergenic
AAGCCAGCCAATGGAGATAG+AGG	0.520626	5_1:-2653002	Msa0710840:CDS
AAGCTGGCAGCTGATGGAGA+AGG	0.643145	5_1:-2653697	Msa0710840:CDS
AATGGGAAGCTGGCAGCTGA+TGG	0.545369	5_1:-2653703	Msa0710840:CDS
AATGGTTTGTCAGCAGCTTC+TGG	0.287143	5_1:-2653514	Msa0710840:CDS
ACCTCAACCTGATCAATATG+AGG	0.437745	5_1:-2653455	Msa0710840:CDS
ACTGCATCTGAGCCTGATGA+AGG	0.598031	5_1:-2652944	Msa0710840:CDS
ACTTTCAAGGGTAGTTGGGT+TGG	0.512734	5_1:+2653798	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CCATACTTAAAGAACTTTCA+AGG	+	chr5_1:2653005-2653024	None:intergenic	30.0%
CCTTGAAAGTTCTTTAAGTA+TGG	-	chr5_1:2653002-2653021	Msa0710840:CDS	30.0%
TGAAAGTTCTTTAAGTATGG+AGG	-	chr5_1:2653005-2653024	Msa0710840:CDS	30.0%
AAAAATGGTGTTCTTCCATA+TGG	+	chr5_1:2653242-2653261	None:intergenic	30.0%
CATTAGGATCACAACAAAAA+TGG	+	chr5_1:2653257-2653276	None:intergenic	30.0%
TATATAGCCAATAAAACCCT+AGG	+	chr5_1:2653449-2653468	None:intergenic	30.0%
AAGAACTTTCAAGGGTAGTT+GGG	+	chr5_1:2652996-2653015	None:intergenic	35.0%
AAAGAACTTTCAAGGGTAGT+TGG	+	chr5_1:2652997-2653016	None:intergenic	35.0%
GAGTAATATTCGTGAAGTGA+AGG	-	chr5_1:2653146-2653165	Msa0710840:intron	35.0%
TTCATCAAAGCAGTCCAAGT+GGG	+	chr5_1:2653219-2653238	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	2652892	2653917	2652892	ID=Msa0710840;Name=Msa0710840
chr5_1	mRNA	2652892	2653917	2652892	ID=Msa0710840-mRNA-1;Parent=Msa0710840;Name=Msa0710840-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0\|0\|0\|1\|1\|1\|2\|0\|201
chr5_1	exon	2653455	2653917	2653455	ID=Msa0710840-mRNA-1:exon:902;Parent=Msa0710840-mRNA-1
chr5_1	exon	2652892	2653034	2652892	ID=Msa0710840-mRNA-1:exon:901;Parent=Msa0710840-mRNA-1
chr5_1	CDS	2653455	2653917	2653455	ID=Msa0710840-mRNA-1:cds;Parent=Msa0710840-mRNA-1
chr5_1	CDS	2652892	2653034	2652892	ID=Msa0710840-mRNA-1:cds;Parent=Msa0710840-mRNA-1

Gene Sequence

>Msa0710840

ATGCAGAACCCTAAACCTAACCCTTCCGAGAATTCAACCCCCACCAACAACAAGTCCCCTCAAGACCCTCCCTCTGATGATGAATCCCCCAAAGAAACCAACCCAACTACCCTTGAAAGTTCTTTAAGTATGGAGGAAGAAGATGTCCTGAAAACCGCTCTCGAAGTCTTAATGGAGTTCAGAGCTCGTGAGAATGGGAAGCTGGCAGCTGATGGAGAAGGTGGAAGTGATGGTCCAGCTATGAAGAGGAGGAAGAGTAATATTCGTGAAGTGAAGGATCATCCTTCTATATATCCAAATTGTTATAGATGCAAACATGTGTTTCCCACTTGGACTGCTTTGATGAACCATATGGAAGAACACCATTTTTGTTGTGATCCTAATGGTTTGTCAGCAGCTTCTGGTTTTTCTGCACTTCCTTCCACATCAACAACCACCCCACCTCAACCTGATCAATATGAGGGTGATGATGAAAGCCAGCCAATGGAGATAGAGGACCCCTCTGATGATGTTGCATCCGAGTCTGATGATACTGCATCTGAGCCTGATGAAGGCGAAGGTTTAGGATTCGACTTGAACGAATTGCCTCCTAATGAAGAAGAGTGA

Protein sequence

>Msa0710840

MQNPKPNPSENSTPTNNKSPQDPPSDDESPKETNPTTLESSLSMEEEDVLKTALEVLMEFRARENGKLAADGEGGSDGPAMKRRKSNIREVKDHPSIYPNCYRCKHVFPTWTALMNHMEEHHFCCDPNGLSAASGFSALPSTSTTTPPQPDQYEGDDESQPMEIEDPSDDVASESDDTASEPDEGEGLGFDLNELPPNEEE*