AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0712400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0712400 MtrunA17_Chr5g0396901 97.424 893 23 0 1 893 25 917 0.0 1804
Msa0712400 MtrunA17_Chr8g0347481 53.333 600 246 8 1 595 1 571 0.0 635
Msa0712400 MtrunA17_Chr4g0042731 29.773 309 168 15 247 539 142 417 9.53e-25 108
Msa0712400 MtrunA17_Chr4g0075541 31.474 251 130 9 280 525 131 344 1.17e-24 107
Msa0712400 MtrunA17_Chr4g0059121 29.845 258 142 10 282 536 104 325 1.12e-23 103
Msa0712400 MtrunA17_Chr4g0065211 30.556 288 150 11 264 535 1139 1392 2.10e-23 107
Msa0712400 MtrunA17_Chr3g0117731 30.233 258 141 9 282 536 103 324 2.35e-23 102
Msa0712400 MtrunA17_Chr5g0406251 26.104 249 146 8 282 528 337 549 4.65e-23 104
Msa0712400 MtrunA17_Chr4g0047191 27.273 253 145 10 282 531 92 308 3.77e-22 99.4
Msa0712400 MtrunA17_Chr4g0047211 27.273 253 145 10 282 531 87 303 3.88e-22 99.0
Msa0712400 MtrunA17_Chr4g0047201 26.616 263 155 9 282 541 248 475 5.69e-22 100
Msa0712400 MtrunA17_Chr2g0305141 29.373 303 166 12 264 549 1101 1372 7.56e-22 102
Msa0712400 MtrunA17_Chr1g0211611 26.908 249 144 8 282 528 343 555 8.06e-22 100
Msa0712400 MtrunA17_Chr5g0397931 28.013 307 165 16 246 534 421 689 8.47e-22 101
Msa0712400 MtrunA17_Chr2g0282321 25.954 262 158 9 282 541 209 436 7.01e-21 97.1
Msa0712400 MtrunA17_Chr6g0461841 31.618 272 152 8 270 536 1001 1243 9.30e-21 98.6
Msa0712400 MtrunA17_Chr6g0450871 28.137 263 143 10 279 526 118 349 1.21e-20 95.1
Msa0712400 MtrunA17_Chr3g0143531 27.092 251 145 9 282 530 67 281 1.52e-20 93.2
Msa0712400 MtrunA17_Chr4g0059181 26.996 263 146 8 279 526 135 366 1.72e-20 94.7
Msa0712400 MtrunA17_Chr7g0244621 29.134 254 143 9 278 523 82 306 1.86e-20 94.4
Msa0712400 MtrunA17_Chr2g0310761 27.687 307 166 12 242 526 933 1205 2.15e-20 97.4
Msa0712400 MtrunA17_Chr3g0117811 25.779 353 192 13 212 526 17 337 2.23e-20 94.0
Msa0712400 MtrunA17_Chr8g0357611 26.687 326 185 11 222 526 1344 1636 3.02e-20 97.1
Msa0712400 MtrunA17_Chr1g0202101 24.315 292 167 10 245 519 273 527 4.23e-20 95.1
Msa0712400 MtrunA17_Chr4g0072741 29.153 295 163 10 247 524 834 1099 6.00e-20 95.9
Msa0712400 MtrunA17_Chr2g0310781 27.213 305 166 13 242 524 839 1109 4.72e-18 89.7
Msa0712400 MtrunA17_Chr4g0068411 28.772 285 152 13 246 516 458 705 4.82e-18 89.4
Msa0712400 MtrunA17_Chr6g0449241 26.316 304 172 12 242 526 20 290 1.25e-17 85.5
Msa0712400 MtrunA17_Chr2g0294351 27.857 280 145 14 265 528 485 723 9.37e-17 85.1
Msa0712400 MtrunA17_Chr3g0116981 25.413 303 169 11 242 523 20 286 1.19e-16 82.8
Msa0712400 MtrunA17_Chr3g0090261 25.101 247 146 9 280 523 126 336 1.29e-16 82.8
Msa0712400 MtrunA17_Chr1g0196631 28.629 248 139 7 289 530 955 1170 1.72e-16 84.7
Msa0712400 MtrunA17_Chr5g0403721 26.531 245 150 9 279 523 28 242 4.05e-16 81.3
Msa0712400 MtrunA17_Chr1g0171571 28.696 230 115 9 326 526 106 315 5.77e-16 82.4
Msa0712400 MtrunA17_Chr5g0443471 27.881 269 156 11 280 541 205 442 9.97e-16 80.9
Msa0712400 MtrunA17_Chr5g0394681 27.461 193 111 6 334 526 201 364 5.12e-15 78.2
Msa0712400 MtrunA17_Chr1g0191171 24.252 301 173 13 246 530 687 948 6.09e-15 79.7
Msa0712400 MtrunA17_Chr5g0427651 25.581 301 165 15 247 528 645 905 1.09e-14 78.6
Msa0712400 MtrunA17_Chr1g0157231 25.789 190 105 6 282 470 188 342 1.95e-14 76.3
Msa0712400 MtrunA17_Chr8g0380551 28.226 248 136 11 282 519 266 481 3.74e-14 76.3
Msa0712400 MtrunA17_Chr1g0179331 24.497 298 172 12 247 528 554 814 8.44e-14 75.9
Msa0712400 MtrunA17_Chr3g0105891 25.820 244 142 8 285 523 371 580 1.26e-13 74.7
Msa0712400 MtrunA17_Chr5g0437741 29.694 229 114 9 282 509 536 718 1.99e-13 74.3
Msa0712400 MtrunA17_Chr4g0000431 23.288 292 182 10 243 521 19 281 2.25e-13 74.3
Msa0712400 MtrunA17_Chr1g0209511 26.766 269 150 11 277 524 352 594 6.35e-13 72.8
Msa0712400 MtrunA17_Chr3g0115171 26.778 239 137 8 277 513 322 524 1.58e-12 71.2
Msa0712400 MtrunA17_Chr1g0171451 28.000 275 149 13 265 526 433 671 1.71e-12 71.2
Msa0712400 MtrunA17_Chr4g0051191 27.273 242 134 8 277 514 203 406 3.86e-12 69.3
Msa0712400 MtrunA17_Chr6g0450571 23.675 283 178 9 243 514 11 266 6.09e-12 69.7
Msa0712400 MtrunA17_Chr8g0359691 26.733 303 147 16 255 523 674 935 6.62e-12 69.7
Msa0712400 MtrunA17_Chr4g0053411 25.298 336 168 15 239 523 458 761 7.31e-12 69.3
Msa0712400 MtrunA17_Chr2g0280191 26.190 252 131 11 241 469 676 895 1.27e-11 68.9
Msa0712400 MtrunA17_Chr1g0209521 24.752 303 166 13 255 527 368 638 1.81e-11 68.2
Msa0712400 MtrunA17_Chr8g0384301 26.293 232 133 8 285 514 349 544 3.38e-11 67.0
Msa0712400 MtrunA17_Chr5g0423121 25.769 260 144 11 277 521 377 602 4.85e-11 66.6
Msa0712400 MtrunA17_Chr4g0041201 26.316 285 160 13 257 531 559 803 4.97e-11 66.6
Msa0712400 MtrunA17_Chr7g0276571 26.592 267 138 12 280 523 457 688 5.48e-11 66.6
Msa0712400 MtrunA17_Chr3g0111551 26.728 217 122 8 285 499 113 294 7.90e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0712400 AT1G64300.2 52.638 777 296 14 1 767 1 715 0.0 787
Msa0712400 AT1G64300.1 52.638 777 296 14 1 767 1 715 0.0 787
Msa0712400 AT5G41730.2 53.306 726 287 11 1 725 1 675 0.0 763
Msa0712400 AT5G41730.1 53.306 726 287 11 1 725 1 675 0.0 763
Msa0712400 AT1G01450.1 53.590 390 151 7 211 594 2 367 1.33e-131 403
Msa0712400 AT1G14000.1 31.392 309 165 17 247 540 161 437 1.74e-24 108
Msa0712400 AT1G62400.1 30.645 248 131 8 280 523 130 340 5.37e-24 105
Msa0712400 AT3G46930.2 25.959 339 198 10 224 545 179 481 7.39e-24 107
Msa0712400 AT3G46930.1 25.959 339 198 10 224 545 144 446 1.25e-23 105
Msa0712400 AT3G46920.1 28.378 370 191 16 192 530 750 1076 1.09e-22 105
Msa0712400 AT3G46920.2 28.378 370 191 16 192 530 816 1142 1.30e-22 105
Msa0712400 AT5G58950.1 27.451 255 140 11 276 523 247 463 1.46e-21 100
Msa0712400 AT2G35050.1 28.618 304 166 14 242 526 968 1239 3.92e-21 100
Msa0712400 AT2G35050.2 28.618 304 166 14 242 526 968 1239 3.92e-21 100
Msa0712400 AT5G01850.1 27.642 246 145 7 282 523 63 279 1.10e-20 94.7
Msa0712400 AT4G38470.2 25.794 252 149 8 282 531 332 547 2.91e-20 96.3
Msa0712400 AT5G57610.1 27.723 303 173 10 247 532 780 1053 3.30e-20 97.1
Msa0712400 AT5G01850.2 29.907 214 120 6 314 523 116 303 1.16e-19 92.0
Msa0712400 AT4G35780.1 24.590 244 146 8 282 523 334 541 1.32e-19 94.4
Msa0712400 AT4G38470.1 25.506 247 146 8 282 526 332 542 1.64e-19 94.0
Msa0712400 AT5G50000.1 26.616 263 147 8 279 526 128 359 1.67e-19 92.0
Msa0712400 AT3G01490.1 28.244 262 144 11 279 526 154 385 2.48e-19 92.0
Msa0712400 AT1G04700.1 30.380 237 137 6 289 524 821 1030 2.58e-19 94.4
Msa0712400 AT1G04700.2 30.380 237 137 6 289 524 821 1030 2.58e-19 94.4
Msa0712400 AT2G17700.1 26.000 250 145 10 282 528 328 540 3.41e-19 92.8
Msa0712400 AT1G79570.2 26.403 303 175 10 242 526 958 1230 3.69e-19 94.0
Msa0712400 AT1G79570.1 26.403 303 175 10 242 526 958 1230 3.69e-19 94.0
Msa0712400 AT3G24715.1 28.873 284 172 9 243 522 842 1099 3.84e-19 93.6
Msa0712400 AT3G24715.3 28.873 284 172 9 243 522 842 1099 3.84e-19 93.6
Msa0712400 AT3G24715.2 28.873 284 172 9 243 522 842 1099 3.84e-19 93.6
Msa0712400 AT1G16270.1 27.143 280 167 8 258 533 890 1136 4.20e-19 93.6
Msa0712400 AT1G16270.2 27.143 280 167 8 258 533 890 1136 4.20e-19 93.6
Msa0712400 AT1G16270.3 27.143 280 167 8 258 533 890 1136 4.20e-19 93.6
Msa0712400 AT4G31170.7 27.092 251 136 9 280 523 175 385 5.51e-19 90.9
Msa0712400 AT4G31170.6 27.092 251 136 9 280 523 175 385 5.51e-19 90.9
Msa0712400 AT4G31170.5 27.092 251 136 9 280 523 175 385 5.51e-19 90.9
Msa0712400 AT4G31170.1 27.092 251 136 9 280 523 175 385 5.51e-19 90.9
Msa0712400 AT4G31170.2 27.092 251 136 9 280 523 175 385 5.51e-19 90.9
Msa0712400 AT4G31170.3 27.092 251 136 9 280 523 175 385 5.51e-19 90.9
Msa0712400 AT4G31170.8 27.092 251 136 9 280 523 175 385 5.51e-19 90.9
Msa0712400 AT3G50730.1 25.403 248 156 8 277 523 77 296 2.34e-18 88.6
Msa0712400 AT4G23050.1 27.815 302 158 14 246 528 465 725 2.70e-18 90.5
Msa0712400 AT3G06630.2 28.283 297 160 15 247 528 433 691 2.76e-18 90.5
Msa0712400 AT4G23050.2 27.815 302 158 14 246 528 466 726 2.80e-18 90.5
Msa0712400 AT5G66710.1 25.641 273 163 8 278 544 113 351 4.54e-18 88.2
Msa0712400 AT2G40860.4 30.964 197 98 6 340 514 24 204 9.78e-18 88.2
Msa0712400 AT5G49470.3 27.857 280 145 14 265 528 510 748 1.04e-17 88.6
Msa0712400 AT5G49470.4 27.857 280 145 14 265 528 484 722 1.12e-17 88.6
Msa0712400 AT2G40860.2 30.144 209 108 6 340 526 122 314 1.39e-17 88.2
Msa0712400 AT5G11850.1 25.676 296 169 12 246 527 607 865 1.50e-17 88.6
Msa0712400 AT5G49470.7 27.857 280 145 14 265 528 383 621 1.50e-17 87.8
Msa0712400 AT3G27560.1 27.600 250 146 10 282 526 71 290 1.61e-17 85.9
Msa0712400 AT2G40860.1 30.964 197 98 6 340 514 122 302 1.64e-17 87.8
Msa0712400 AT2G40860.3 30.964 197 98 6 340 514 122 302 1.69e-17 87.8
Msa0712400 AT5G49470.1 27.857 280 145 14 265 528 223 461 2.16e-17 86.7
Msa0712400 AT2G24360.1 26.050 238 143 7 286 523 180 384 2.38e-17 85.9
Msa0712400 AT5G40540.1 27.668 253 148 11 276 523 64 286 3.00e-17 84.7
Msa0712400 AT3G22750.1 24.528 265 151 8 279 526 120 352 3.75e-17 85.1
Msa0712400 AT3G57830.3 27.737 274 162 10 279 527 398 660 5.95e-17 86.3
Msa0712400 AT3G57830.1 27.737 274 162 10 279 527 398 660 5.95e-17 86.3
Msa0712400 AT4G18950.2 28.571 252 138 10 282 526 200 416 8.25e-17 84.7
Msa0712400 AT4G18950.1 28.571 252 138 10 282 526 200 416 9.92e-17 84.3
Msa0712400 AT3G63260.1 24.060 266 155 8 279 526 129 365 1.26e-16 83.6
Msa0712400 AT4G24480.1 26.073 303 166 13 247 528 668 933 1.71e-16 85.1
Msa0712400 AT5G50180.1 27.236 246 145 9 282 523 65 280 2.08e-16 82.4
Msa0712400 AT1G67890.1 27.957 279 145 15 265 528 506 743 2.22e-16 84.3
Msa0712400 AT2G42290.1 28.731 268 159 9 282 527 389 646 2.22e-16 84.3
Msa0712400 AT5G40540.3 29.439 214 120 9 314 523 3 189 4.52e-16 79.7
Msa0712400 AT4G24480.2 25.589 297 170 13 247 528 668 928 4.71e-16 83.6
Msa0712400 AT4G24480.3 25.589 297 170 13 247 528 668 928 5.67e-16 83.2
Msa0712400 AT5G40540.2 29.439 214 120 9 314 523 24 210 7.08e-16 79.3
Msa0712400 AT3G06620.1 27.798 277 149 13 265 528 513 751 8.40e-16 82.8
Msa0712400 AT3G50720.1 26.848 257 159 9 276 531 88 316 1.56e-15 80.1
Msa0712400 AT3G58760.1 29.000 300 156 18 247 526 165 427 6.22e-15 79.0
Msa0712400 AT3G58760.3 29.000 300 156 18 247 526 228 490 8.82e-15 79.0
Msa0712400 AT3G58760.5 29.000 300 156 18 247 526 228 490 8.82e-15 79.0
Msa0712400 AT3G06630.1 28.261 276 145 15 247 507 433 670 8.98e-15 79.0
Msa0712400 AT4G14780.1 26.316 190 109 5 339 526 178 338 1.09e-14 77.4
Msa0712400 AT3G46930.4 24.715 263 149 8 224 473 179 405 3.29e-14 76.3
Msa0712400 AT3G46930.3 24.715 263 149 8 224 473 179 405 3.40e-14 76.3
Msa0712400 AT2G31800.1 25.987 304 177 14 243 530 188 459 4.10e-14 76.3
Msa0712400 AT3G46930.5 24.715 263 149 8 224 473 144 370 5.91e-14 75.5
Msa0712400 AT3G46930.6 24.715 263 149 8 224 473 83 309 6.99e-14 74.3
Msa0712400 AT3G58640.1 28.205 273 146 13 257 519 565 797 8.08e-14 76.3
Msa0712400 AT3G58640.2 28.205 273 146 13 257 519 565 797 8.08e-14 76.3
Msa0712400 AT1G50240.3 24.211 285 161 12 243 513 1 244 2.06e-13 75.1
Msa0712400 AT1G50240.2 24.211 285 161 12 243 513 1 244 2.06e-13 75.1
Msa0712400 AT5G01890.1 23.743 358 209 13 191 527 646 960 3.24e-13 74.3
Msa0712400 AT5G66850.1 25.703 249 153 10 283 530 394 611 3.25e-13 74.3
Msa0712400 AT5G66850.2 25.703 249 153 10 283 530 394 611 3.88e-13 73.9
Msa0712400 AT3G59830.1 27.586 290 162 13 247 519 193 451 4.67e-13 73.2
Msa0712400 AT3G06630.6 28.571 238 120 13 247 470 349 550 5.22e-13 73.2
Msa0712400 AT3G06630.4 28.571 238 120 13 247 470 433 634 5.38e-13 73.2
Msa0712400 AT3G06630.3 28.571 238 120 13 247 470 433 634 5.38e-13 73.2
Msa0712400 AT3G06630.5 28.571 238 120 13 247 470 433 634 5.38e-13 73.2
Msa0712400 AT5G18700.1 25.847 236 142 10 285 520 46 248 7.50e-13 73.6
Msa0712400 AT1G67890.3 27.907 258 130 15 265 507 506 722 3.22e-12 70.9
Msa0712400 AT1G67890.2 27.907 258 130 15 265 507 506 722 3.22e-12 70.9
Msa0712400 AT1G67890.4 27.907 258 130 15 265 507 378 594 4.32e-12 70.5
Msa0712400 AT2G31010.1 25.685 292 159 13 257 538 531 774 5.45e-12 70.5
Msa0712400 AT2G31010.2 25.685 292 159 13 257 538 531 774 5.45e-12 70.5
Msa0712400 AT3G53930.2 27.586 261 134 13 247 491 19 240 6.51e-12 70.1
Msa0712400 AT3G53930.1 27.586 261 134 13 247 491 19 240 6.62e-12 70.1
Msa0712400 AT5G49470.5 28.182 220 104 12 265 469 510 690 9.12e-12 69.3
Msa0712400 AT5G49470.6 28.182 220 104 12 265 469 510 690 9.72e-12 69.3
Msa0712400 AT2G42640.1 28.044 271 141 13 265 524 547 774 1.17e-11 69.3
Msa0712400 AT2G42640.4 28.044 271 141 13 265 524 509 736 1.33e-11 68.9
Msa0712400 AT5G38210.2 25.175 286 162 10 280 544 409 663 1.58e-11 68.6
Msa0712400 AT5G38210.3 24.739 287 162 10 280 544 391 645 1.62e-11 68.6
Msa0712400 AT5G07140.1 26.014 296 177 13 242 528 321 583 2.51e-11 67.8
Msa0712400 AT3G01490.2 27.632 228 122 10 279 492 154 352 3.00e-11 66.6
Msa0712400 AT4G08480.1 26.172 256 147 9 261 514 529 744 3.01e-11 67.8
Msa0712400 AT5G38210.1 25.175 286 162 10 280 544 397 651 5.02e-11 67.0
Msa0712400 AT5G40540.4 28.061 196 114 8 276 469 64 234 5.18e-11 65.1
Msa0712400 AT4G08500.1 26.336 262 153 9 255 514 353 576 5.46e-11 67.0
Msa0712400 AT1G69270.1 25.820 244 144 10 270 510 291 500 5.59e-11 66.6
Msa0712400 AT3G02880.1 31.500 200 99 9 272 467 375 540 6.20e-11 66.6
Msa0712400 AT3G19700.1 28.497 193 105 8 282 469 730 894 6.57e-11 67.0

Find 163 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTCCTGCTTTGATATTT+TGG 0.080066 5_1:+4236312 None:intergenic
CTCTCAGATTCTGAGACTTT+TGG 0.146039 5_1:+4235461 None:intergenic
ATCAAACTCACAAGATATTT+TGG 0.164131 5_1:+4236266 None:intergenic
TTGATTCCCTTTGTTAATAT+TGG 0.196770 5_1:+4235433 None:intergenic
CAAGCAAAAGTTGTTGGATT+TGG 0.204645 5_1:-4236593 Msa0712400:CDS
GAGATTTGGCAAACAATATT+TGG 0.205668 5_1:-4237266 Msa0712400:CDS
GATCCTAACAAAACACTAAT+TGG 0.207436 5_1:+4237037 None:intergenic
AATTGCTTGCAAATTTCTTT+TGG 0.212048 5_1:+4237241 None:intergenic
TCTCCTCACTTGGTAATCTT+TGG 0.230174 5_1:+4237015 None:intergenic
GCTTATAAAGTTGTTGAAAA+AGG 0.240086 5_1:-4235993 Msa0712400:CDS
GTGTCATCAGAAATTGATTT+TGG 0.241755 5_1:+4235798 None:intergenic
GTAGGATATTACAAGGATTA+AGG 0.243595 5_1:+4236648 None:intergenic
TATTCCTACTATAGATAATA+AGG 0.252638 5_1:-4235937 Msa0712400:CDS
AAGAGATTAACCCTATAATT+TGG 0.257480 5_1:-4236499 Msa0712400:CDS
GTCCTCTTAGAGAGCTTTCC+AGG 0.263161 5_1:-4237549 Msa0712400:CDS
AACTGACCTTGGTCTTTCTT+TGG 0.265277 5_1:+4235708 None:intergenic
GGTTACCAGATCTTTAATTA+TGG 0.266121 5_1:+4235824 None:intergenic
CATAGATATTGCAATATGTT+TGG 0.282076 5_1:+4236866 None:intergenic
TCAGGTGCATACCAAATTAT+AGG 0.292831 5_1:+4236488 None:intergenic
CTGGTTTCTCATCTATTTGT+AGG 0.294229 5_1:-4236208 Msa0712400:CDS
TTTGTCTTTAACCTGTTAAA+TGG 0.297516 5_1:+4235308 None:intergenic
CGGCACATTGGTGAGGTTCT+TGG 0.303933 5_1:-4237736 Msa0712400:CDS
TATGGTCATGTCTCAGATTA+AGG 0.304997 5_1:-4234981 None:intergenic
TGGAAGTCTAAAAGCGCTTA+TGG 0.306735 5_1:-4237716 Msa0712400:CDS
TGAAACATGGCAGCATCTTC+TGG 0.314429 5_1:+4235151 None:intergenic
AAAAGAAATTTGCAAGCAAT+TGG 0.319977 5_1:-4237239 Msa0712400:CDS
GGATTGTCATCTGTTGAAAA+TGG 0.333696 5_1:-4236572 Msa0712400:CDS
ATTCTTTGAAGAAGTGAGTT+TGG 0.334226 5_1:+4237153 None:intergenic
GTCCTTTGATTGCTCACATA+TGG 0.339488 5_1:+4235065 None:intergenic
AGCATTGTGAGGATGCAAAA+AGG 0.341134 5_1:-4235215 Msa0712400:CDS
CATTATCTGGTCGGGTTTCA+AGG 0.346406 5_1:-4235547 Msa0712400:CDS
ATTATACATTAAGCATTGTT+TGG 0.350263 5_1:-4237509 Msa0712400:CDS
TTGACACGTCTTATTCTTGT+TGG 0.355333 5_1:+4235251 None:intergenic
GAAATTGATGAATGGTAATT+CGG 0.363355 5_1:-4237101 Msa0712400:CDS
TCGGGTTTCAAGGACAAATA+AGG 0.364030 5_1:-4235537 Msa0712400:CDS
TTTAAGGAAATTCAATGGTT+AGG 0.370820 5_1:-4236971 Msa0712400:CDS
GAGACCAATCACCATTTAAC+AGG 0.371640 5_1:-4235319 Msa0712400:CDS
ATTGTTTGGATTCAAAAGAT+TGG 0.378122 5_1:-4237495 Msa0712400:CDS
GTGAGAAAGTGATAACAAAT+TGG 0.378799 5_1:+4236889 None:intergenic
TTCTTTGGCTTCATTTGTGA+TGG 0.379395 5_1:+4235723 None:intergenic
TTAACCTGTTAAATGGTGAT+TGG 0.382051 5_1:+4235315 None:intergenic
ATAGATATTGCAATATGTTT+GGG 0.387928 5_1:+4236867 None:intergenic
AGTTCAAGCTGATTTAGTTC+AGG 0.389760 5_1:+4236128 None:intergenic
ATCTCATGCTTCAAATGGCC+AGG 0.399868 5_1:-4236712 Msa0712400:CDS
TTGAAGACAGAACTACAAAA+TGG 0.399911 5_1:-4237585 Msa0712400:CDS
TGAGGAGAAGATTGGGAAGG+GGG 0.403016 5_1:-4237000 Msa0712400:CDS
GGGTTAATCTCTTCACCAAT+TGG 0.403263 5_1:+4236509 None:intergenic
ATTGTAACATTCTTCTTTGA+CGG 0.405231 5_1:+4235753 None:intergenic
CCATATGTTGTTCTTACATA+AGG 0.409101 5_1:+4234999 None:intergenic
TACCAAGTGAGGAGAAGATT+GGG 0.409714 5_1:-4237007 Msa0712400:CDS
ACAGTAGCCACATCCTTTGT+TGG 0.413590 5_1:+4235900 None:intergenic
TTGGATTCAAAAGATTGGTC+AGG 0.420334 5_1:-4237490 Msa0712400:CDS
TCATTACCACCAACATTATC+TGG 0.424357 5_1:-4235560 Msa0712400:CDS
ATGCTGCCATGTTTCAGATA+TGG 0.424537 5_1:-4235144 Msa0712400:CDS
GAGAATGCTGGGGAGATGTC+AGG 0.427765 5_1:-4237376 Msa0712400:CDS
TTATAAGCAAACATTTCATA+AGG 0.434328 5_1:+4236008 None:intergenic
CATCTTCACAAACTGTGCTT+TGG 0.436780 5_1:+4235856 None:intergenic
TGCATCCATAATTAAAGATC+TGG 0.439192 5_1:-4235829 Msa0712400:CDS
CTCAAAGGGCTCAATGCTAA+TGG 0.444928 5_1:+4235023 None:intergenic
CTCAAAGGACTCAATGGAAA+TGG 0.446507 5_1:+4235089 None:intergenic
TTCCCAATCTTCTCCTCACT+TGG 0.447928 5_1:+4237005 None:intergenic
ATTCCTACTATAGATAATAA+GGG 0.450157 5_1:-4235936 Msa0712400:CDS
TGAAAATGATTATCCTCAAA+AGG 0.452553 5_1:+4236350 None:intergenic
GCAAGCAATTGGAGAATGCT+TGG 0.459665 5_1:-4237228 Msa0712400:CDS
TTGTAACATTCTTCTTTGAC+GGG 0.462036 5_1:+4235754 None:intergenic
GAGAACAAAATCTGTCTCCT+TGG 0.464084 5_1:+4236752 None:intergenic
GGAGATGTCAGGGTTGGATC+AGG 0.464317 5_1:-4237365 Msa0712400:CDS
TTACCAAGTGAGGAGAAGAT+TGG 0.469923 5_1:-4237008 Msa0712400:CDS
AGGAATTTAAGGAAATTCAA+TGG 0.476317 5_1:-4236976 Msa0712400:CDS
ACCACCAACATTATCTGGTC+GGG 0.478543 5_1:-4235555 Msa0712400:CDS
CACCGCCAATGAGTCCTTCA+AGG 0.481111 5_1:-4235181 Msa0712400:CDS
AAAGCCGATGCATATAGCTT+TGG 0.483467 5_1:-4236407 Msa0712400:CDS
CAGGTGCATACCAAATTATA+GGG 0.486379 5_1:+4236489 None:intergenic
TCACCAACACATAATCCAAT+TGG 0.489553 5_1:-4236524 Msa0712400:CDS
TTCACCTTCTTTGAATACCC+TGG 0.491717 5_1:+4237531 None:intergenic
TACAAGGATTAAGGTTTCCA+TGG 0.498240 5_1:+4236657 None:intergenic
TATGGACTCAAAGGACTCAA+TGG 0.498751 5_1:+4235083 None:intergenic
CTACCCTTATTATCTATAGT+AGG 0.503856 5_1:+4235933 None:intergenic
ACCAACCAAAATATCAAAGC+AGG 0.507591 5_1:-4236317 Msa0712400:CDS
GTTCTTACATAAGGACTCAA+AGG 0.508350 5_1:+4235008 None:intergenic
ATTGAAGCGATCGAGAATGC+TGG 0.508806 5_1:-4237388 Msa0712400:CDS
CTTTCCAGGGTATTCAAAGA+AGG 0.511780 5_1:-4237535 Msa0712400:CDS
TCTAGCTCAGTTAAAACCTC+AGG 0.514317 5_1:+4236470 None:intergenic
TTGAAGCGATCGAGAATGCT+GGG 0.515187 5_1:-4237387 Msa0712400:CDS
ACTGATCCGTCTCAGCGTCC+TGG 0.518950 5_1:-4236227 Msa0712400:CDS
CTTCTGGTGTATGACCTTGA+AGG 0.519264 5_1:+4235167 None:intergenic
AGAATGCTTGGAGAGAAGAT+AGG 0.519476 5_1:-4237216 Msa0712400:CDS
AGTTGATGAACAAGGATTTG+TGG 0.524686 5_1:-4236799 Msa0712400:CDS
TGGATTCAAAAGATTGGTCA+GGG 0.524926 5_1:-4237489 Msa0712400:CDS
TGCTGGGGAGATGTCAGGGT+TGG 0.526191 5_1:-4237371 Msa0712400:CDS
GGAAATCCAATATTAACAAA+GGG 0.528711 5_1:-4235439 Msa0712400:CDS
CCTTATGTAAGAACAACATA+TGG 0.529072 5_1:-4234999 Msa0712400:CDS
CTATAAATCCAGTTAAAAGA+AGG 0.531172 5_1:-4235487 Msa0712400:CDS
AGGAAATCCAATATTAACAA+AGG 0.532942 5_1:-4235440 Msa0712400:CDS
TATGACCTTGAAGGACTCAT+TGG 0.538589 5_1:+4235176 None:intergenic
CAAGAAAATATGATGTGGAA+TGG 0.539842 5_1:-4237312 Msa0712400:CDS
ACACAATCGCAGATGAGATA+AGG 0.544064 5_1:-4237621 Msa0712400:CDS
GTGCCGGAATGGAACAGCTC+CGG 0.546196 5_1:-4237756 None:intergenic
TTTGTTCTCTATACCTGTGG+TGG 0.547282 5_1:-4236738 Msa0712400:CDS
GTAGATTGTTGTGACATTGA+AGG 0.548073 5_1:-4236092 Msa0712400:CDS
ATATTATATGTCCTCTCCAT+AGG 0.548736 5_1:+4236050 None:intergenic
ATGCTTGGAGAGAAGATAGG+TGG 0.548742 5_1:-4237213 Msa0712400:CDS
TATAATTTGGTATGCACCTG+AGG 0.550991 5_1:-4236486 Msa0712400:CDS
TTCTTACATAAGGACTCAAA+GGG 0.551714 5_1:+4235009 None:intergenic
GGTTGATCTCATGCTTCAAA+TGG 0.555778 5_1:-4236717 Msa0712400:CDS
AACATATTGCAATATCTATG+TGG 0.560041 5_1:-4236863 Msa0712400:CDS
ACCCTGGAAAGCTCTCTAAG+AGG 0.560256 5_1:+4237547 None:intergenic
ATGGAACAGCTCCGGCACAT+TGG 0.561500 5_1:-4237748 Msa0712400:CDS
AGCAATCAAAGGACTAGAAG+AGG 0.562315 5_1:-4235056 Msa0712400:CDS
GGCGAGCGATATGGAAACAA+AGG 0.564210 5_1:+4236287 None:intergenic
CAAGAACCTCACCAATGTGC+CGG 0.564531 5_1:+4237737 None:intergenic
GTGGAGATATTCAATGCCCC+TGG 0.567677 5_1:+4236694 None:intergenic
TCAATGTCACAACAATCTAC+AGG 0.568956 5_1:+4236095 None:intergenic
GACCTTGAAGGACTCATTGG+CGG 0.570717 5_1:+4235179 None:intergenic
TTGTTGAAGAAATTGATGAA+TGG 0.570718 5_1:-4237109 Msa0712400:CDS
TCACCAATTGGATTATGTGT+TGG 0.571772 5_1:+4236521 None:intergenic
GAGAAACCAGGACGCTGAGA+CGG 0.572704 5_1:+4236221 None:intergenic
TACCACCAACATTATCTGGT+CGG 0.572730 5_1:-4235556 Msa0712400:CDS
GTCTGAGCCAACAAAGGATG+TGG 0.574953 5_1:-4235907 Msa0712400:CDS
TTGCTCACATATGGACTCAA+AGG 0.582014 5_1:+4235074 None:intergenic
TCTCATGCTTCAAATGGCCA+GGG 0.583448 5_1:-4236711 Msa0712400:CDS
AGTGAGGAGAAGATTGGGAA+GGG 0.587219 5_1:-4237002 Msa0712400:CDS
TCGATCGCTTCAATAACCGC+AGG 0.587690 5_1:+4237397 None:intergenic
GTGAGGAGAAGATTGGGAAG+GGG 0.591731 5_1:-4237001 Msa0712400:CDS
GAGGCTCATAGAAGCACTCA+AGG 0.595114 5_1:-4237191 Msa0712400:CDS
GTCCATATGTGAGCAATCAA+AGG 0.596259 5_1:-4235067 Msa0712400:CDS
CATACCAAAGCTATATGCAT+CGG 0.599646 5_1:+4236403 None:intergenic
AAAGCTAGAAACTCACAAGA+AGG 0.600879 5_1:-4236623 Msa0712400:CDS
AACTTGATGTCAATGATGAC+TGG 0.601724 5_1:+4235510 None:intergenic
AGAATGCTGGGGAGATGTCA+GGG 0.601811 5_1:-4237375 Msa0712400:CDS
TGAGAGAGAATTGTGGTCCA+AGG 0.604253 5_1:-4236769 Msa0712400:CDS
TCCTCTTAGAGAGCTTTCCA+GGG 0.606681 5_1:-4237548 Msa0712400:CDS
TTTCTCTGAGTACTTACAAG+AGG 0.613541 5_1:+4236427 None:intergenic
ACCCGACCAGATAATGTTGG+TGG 0.618092 5_1:+4235554 None:intergenic
AGCATGAGATCAACCACCAC+AGG 0.618436 5_1:+4236725 None:intergenic
CTTGGAGAGAAGATAGGTGG+AGG 0.618932 5_1:-4237210 Msa0712400:CDS
AAAATATCAAAGCAGGAGAG+AGG 0.620693 5_1:-4236310 Msa0712400:CDS
GAAACCCGACCAGATAATGT+TGG 0.622199 5_1:+4235551 None:intergenic
CTACAAATAGATGAGAAACC+AGG 0.623694 5_1:+4236209 None:intergenic
AACTATCCATATCTGAAACA+TGG 0.625924 5_1:+4235138 None:intergenic
ACAGTAATGATAAGGTATTG+TGG 0.632731 5_1:-4237060 Msa0712400:CDS
ATGTCACAACAATCTACAGG+TGG 0.639302 5_1:+4236098 None:intergenic
ATGCTAGAGTGAATATGTCG+TGG 0.640538 5_1:+4237647 None:intergenic
AGGATTGATCAAAACATACT+CGG 0.640765 5_1:+4236148 None:intergenic
CAAAAGTCTCAGAATCTGAG+AGG 0.650694 5_1:-4235460 Msa0712400:CDS
TCTCTAAGAGGACATTCAAG+AGG 0.651164 5_1:+4237559 None:intergenic
ACAGCTCCGGCACATTGGTG+AGG 0.651948 5_1:-4237743 Msa0712400:CDS
GCTTGCAAGAAAATATGATG+TGG 0.653459 5_1:-4237317 Msa0712400:CDS
TAGTGAGTCTGAGCCAACAA+AGG 0.653550 5_1:-4235913 Msa0712400:CDS
CGATCGCTTCAATAACCGCA+GGG 0.654532 5_1:+4237398 None:intergenic
AAGTGAGGAGAAGATTGGGA+AGG 0.655591 5_1:-4237003 Msa0712400:CDS
AATTGGATTATGTGTTGGTG+AGG 0.655784 5_1:+4236526 None:intergenic
TGTGACGGAGTTGATGAACA+AGG 0.656381 5_1:-4236807 Msa0712400:CDS
CTCATGCTTCAAATGGCCAG+GGG 0.668271 5_1:-4236710 Msa0712400:CDS
GATCCAAAGATTACCAAGTG+AGG 0.673638 5_1:-4237018 Msa0712400:CDS
ATGAAGCCAAAGAAAGACCA+AGG 0.681190 5_1:-4235714 Msa0712400:intron
TGTAACATTCTTCTTTGACG+GGG 0.685372 5_1:+4235755 None:intergenic
GTACTAACAACAGCATTGTG+AGG 0.690491 5_1:-4235226 Msa0712400:CDS
GATAGTTCTTTGAAGATACG+AGG 0.692687 5_1:-4235122 Msa0712400:CDS
TGTCACAACAATCTACAGGT+GGG 0.697110 5_1:+4236099 None:intergenic
CTTGCTTTGCTATTTCCCTG+CGG 0.700742 5_1:-4237413 Msa0712400:CDS
TGAAGCGATCGAGAATGCTG+GGG 0.704654 5_1:-4237386 Msa0712400:CDS
ACATACATGAGAGAGAATTG+TGG 0.732943 5_1:-4236776 Msa0712400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATAGC+AGG + chr5_1:4237076-4237095 None:intergenic 10.0%
!!! ATTATACATTAAGCATTGTT+TGG - chr5_1:4235222-4235241 Msa0712400:CDS 20.0%
!! ATAGATATTGCAATATGTTT+GGG + chr5_1:4235867-4235886 None:intergenic 20.0%
!! TTATAAGCAAACATTTCATA+AGG + chr5_1:4236726-4236745 None:intergenic 20.0%
!! CTTATAAAGTTGTTGAAAAA+GGG - chr5_1:4236739-4236758 Msa0712400:CDS 20.0%
!! TATTCCTACTATAGATAATA+AGG - chr5_1:4236794-4236813 Msa0712400:CDS 20.0%
!! ATTCCTACTATAGATAATAA+GGG - chr5_1:4236795-4236814 Msa0712400:CDS 20.0%
!!! TCAGTTAGAAAAAAAGTATA+TGG - chr5_1:4237345-4237364 Msa0712400:CDS 20.0%
! ATTGTTTGGATTCAAAAGAT+TGG - chr5_1:4235236-4235255 Msa0712400:CDS 25.0%
!! AATTGCTTGCAAATTTCTTT+TGG + chr5_1:4235493-4235512 None:intergenic 25.0%
! AAAAGAAATTTGCAAGCAAT+TGG - chr5_1:4235492-4235511 Msa0712400:CDS 25.0%
! TTGTTGAAGAAATTGATGAA+TGG - chr5_1:4235622-4235641 Msa0712400:intron 25.0%
! GAAATTGATGAATGGTAATT+CGG - chr5_1:4235630-4235649 Msa0712400:intron 25.0%
! TCACAAAAACAGTAATGATA+AGG - chr5_1:4235663-4235682 Msa0712400:intron 25.0%
! AGGAATTTAAGGAAATTCAA+TGG - chr5_1:4235755-4235774 Msa0712400:CDS 25.0%
! TTTAAGGAAATTCAATGGTT+AGG - chr5_1:4235760-4235779 Msa0712400:CDS 25.0%
! CATAGATATTGCAATATGTT+TGG + chr5_1:4235868-4235887 None:intergenic 25.0%
! AACATATTGCAATATCTATG+TGG - chr5_1:4235868-4235887 Msa0712400:CDS 25.0%
!!! TATTTTCAAGCAAAAGTTGT+TGG - chr5_1:4236132-4236151 Msa0712400:CDS 25.0%
! AAGAGATTAACCCTATAATT+TGG - chr5_1:4236232-4236251 Msa0712400:CDS 25.0%
! TGAAAATGATTATCCTCAAA+AGG + chr5_1:4236384-4236403 None:intergenic 25.0%
!! TTTGAGGATAATCATTTTCA+AGG - chr5_1:4236384-4236403 Msa0712400:CDS 25.0%
!! TTGAGGATAATCATTTTCAA+GGG - chr5_1:4236385-4236404 Msa0712400:CDS 25.0%
!! ATCAAACTCACAAGATATTT+TGG + chr5_1:4236468-4236487 None:intergenic 25.0%
! TGAGTTTGATCAAAAAATGT+TGG - chr5_1:4236478-4236497 Msa0712400:CDS 25.0%
! GCTTATAAAGTTGTTGAAAA+AGG - chr5_1:4236738-4236757 Msa0712400:CDS 25.0%
!!! ATCAGAAATTGATTTTGGAT+AGG + chr5_1:4236931-4236950 None:intergenic 25.0%
! ATTGTAACATTCTTCTTTGA+CGG + chr5_1:4236981-4237000 None:intergenic 25.0%
! CAATAAAATTAAACTGACCT+TGG + chr5_1:4237037-4237056 None:intergenic 25.0%
! TGTATTGAATTGATTGAAAC+AGG - chr5_1:4237123-4237142 Msa0712400:CDS 25.0%
! CTATAAATCCAGTTAAAAGA+AGG - chr5_1:4237244-4237263 Msa0712400:CDS 25.0%
! AGGAAATCCAATATTAACAA+AGG - chr5_1:4237291-4237310 Msa0712400:CDS 25.0%
! GGAAATCCAATATTAACAAA+GGG - chr5_1:4237292-4237311 Msa0712400:CDS 25.0%
! TTGATTCCCTTTGTTAATAT+TGG + chr5_1:4237301-4237320 None:intergenic 25.0%
!! GTTAGAAAAAAAGTATATGG+AGG - chr5_1:4237348-4237367 Msa0712400:CDS 25.0%
!!! CTTGTTTTCAGATTTAAACT+AGG + chr5_1:4237393-4237412 None:intergenic 25.0%
! TTTGTCTTTAACCTGTTAAA+TGG + chr5_1:4237426-4237445 None:intergenic 25.0%
TTGAAGACAGAACTACAAAA+TGG - chr5_1:4235146-4235165 Msa0712400:CDS 30.0%
CAAGAAAATATGATGTGGAA+TGG - chr5_1:4235419-4235438 Msa0712400:CDS 30.0%
!!! ATGATCCAAAACTTTTTCAG+TGG - chr5_1:4235443-4235462 Msa0712400:CDS 30.0%
! AATCTCCACTGAAAAAGTTT+TGG + chr5_1:4235451-4235470 None:intergenic 30.0%
!!! AAACTTTTTCAGTGGAGATT+TGG - chr5_1:4235451-4235470 Msa0712400:CDS 30.0%
GAGATTTGGCAAACAATATT+TGG - chr5_1:4235465-4235484 Msa0712400:CDS 30.0%
! ATTCTTTGAAGAAGTGAGTT+TGG + chr5_1:4235581-4235600 None:intergenic 30.0%
!! ACAGTAATGATAAGGTATTG+TGG - chr5_1:4235671-4235690 Msa0712400:intron 30.0%
GATCCTAACAAAACACTAAT+TGG + chr5_1:4235697-4235716 None:intergenic 30.0%
GTGAGAAAGTGATAACAAAT+TGG + chr5_1:4235845-4235864 None:intergenic 30.0%
! GTTTCCATGGTAAATGTTTT+TGG + chr5_1:4236064-4236083 None:intergenic 30.0%
GTAGGATATTACAAGGATTA+AGG + chr5_1:4236086-4236105 None:intergenic 30.0%
! GCTTTTAGTAGGATATTACA+AGG + chr5_1:4236093-4236112 None:intergenic 30.0%
!!! ATATGTTTTGAGTTGTTGAC+AGG - chr5_1:4236351-4236370 Msa0712400:CDS 30.0%
!!! TATGTTTTGAGTTGTTGACA+GGG - chr5_1:4236352-4236371 Msa0712400:CDS 30.0%
AGGATTGATCAAAACATACT+CGG + chr5_1:4236586-4236605 None:intergenic 30.0%
! TTTTCTCAAGAGTTTTCCTA+TGG - chr5_1:4236665-4236684 Msa0712400:CDS 30.0%
ATATTATATGTCCTCTCCAT+AGG + chr5_1:4236684-4236703 None:intergenic 30.0%
CTACCCTTATTATCTATAGT+AGG + chr5_1:4236801-4236820 None:intergenic 30.0%
TGCATCCATAATTAAAGATC+TGG - chr5_1:4236902-4236921 Msa0712400:CDS 30.0%
GGTTACCAGATCTTTAATTA+TGG + chr5_1:4236910-4236929 None:intergenic 30.0%
!! GTGTCATCAGAAATTGATTT+TGG + chr5_1:4236936-4236955 None:intergenic 30.0%
TTGTAACATTCTTCTTTGAC+GGG + chr5_1:4236980-4236999 None:intergenic 30.0%
!! GATTTTGTTGCTTGCAATTT+AGG + chr5_1:4237153-4237172 None:intergenic 30.0%
TTAACCTGTTAAATGGTGAT+TGG + chr5_1:4237419-4237438 None:intergenic 30.0%
! AACTATCCATATCTGAAACA+TGG + chr5_1:4237596-4237615 None:intergenic 30.0%
TTCTTACATAAGGACTCAAA+GGG + chr5_1:4237725-4237744 None:intergenic 30.0%
CCATATGTTGTTCTTACATA+AGG + chr5_1:4237735-4237754 None:intergenic 30.0%
CCTTATGTAAGAACAACATA+TGG - chr5_1:4237732-4237751 Msa0712400:CDS 30.0%
! TTGGATTCAAAAGATTGGTC+AGG - chr5_1:4235241-4235260 Msa0712400:CDS 35.0%
! TGGATTCAAAAGATTGGTCA+GGG - chr5_1:4235242-4235261 Msa0712400:CDS 35.0%
GCTTGCAAGAAAATATGATG+TGG - chr5_1:4235414-4235433 Msa0712400:CDS 35.0%
!! TGGCCAATTAGTGTTTTGTT+AGG - chr5_1:4235691-4235710 Msa0712400:intron 35.0%
! GAAAGAGTTTTCTCTTGTGA+CGG - chr5_1:4235909-4235928 Msa0712400:CDS 35.0%
!! AGTTGATGAACAAGGATTTG+TGG - chr5_1:4235932-4235951 Msa0712400:CDS 35.0%
ACATACATGAGAGAGAATTG+TGG - chr5_1:4235955-4235974 Msa0712400:CDS 35.0%
! GATTTTGTTCTCTATACCTG+TGG - chr5_1:4235990-4236009 Msa0712400:CDS 35.0%
!!! CATGGTAAATGTTTTTGGAG+TGG + chr5_1:4236059-4236078 None:intergenic 35.0%
CACTCCAAAAACATTTACCA+TGG - chr5_1:4236057-4236076 Msa0712400:CDS 35.0%
TACAAGGATTAAGGTTTCCA+TGG + chr5_1:4236077-4236096 None:intergenic 35.0%
!!! GTGAGTTTCTAGCTTTTAGT+AGG + chr5_1:4236104-4236123 None:intergenic 35.0%
AAAGCTAGAAACTCACAAGA+AGG - chr5_1:4236108-4236127 Msa0712400:CDS 35.0%
CAAGCAAAAGTTGTTGGATT+TGG - chr5_1:4236138-4236157 Msa0712400:CDS 35.0%
GGATTGTCATCTGTTGAAAA+TGG - chr5_1:4236159-4236178 Msa0712400:CDS 35.0%
! AATTGGATTATGTGTTGGTG+AGG + chr5_1:4236208-4236227 None:intergenic 35.0%
TCACCAACACATAATCCAAT+TGG - chr5_1:4236207-4236226 Msa0712400:CDS 35.0%
TCACCAATTGGATTATGTGT+TGG + chr5_1:4236213-4236232 None:intergenic 35.0%
CAGGTGCATACCAAATTATA+GGG + chr5_1:4236245-4236264 None:intergenic 35.0%
TCAGGTGCATACCAAATTAT+AGG + chr5_1:4236246-4236265 None:intergenic 35.0%
TATAATTTGGTATGCACCTG+AGG - chr5_1:4236245-4236264 Msa0712400:CDS 35.0%
TTTCTCTGAGTACTTACAAG+AGG + chr5_1:4236307-4236326 None:intergenic 35.0%
CATACCAAAGCTATATGCAT+CGG + chr5_1:4236331-4236350 None:intergenic 35.0%
ACCAACCAAAATATCAAAGC+AGG - chr5_1:4236414-4236433 Msa0712400:CDS 35.0%
!!! TCCTGCTTTGATATTTTGGT+TGG + chr5_1:4236418-4236437 None:intergenic 35.0%
! TCTCTCCTGCTTTGATATTT+TGG + chr5_1:4236422-4236441 None:intergenic 35.0%
AAAATATCAAAGCAGGAGAG+AGG - chr5_1:4236421-4236440 Msa0712400:CDS 35.0%
CTACAAATAGATGAGAAACC+AGG + chr5_1:4236525-4236544 None:intergenic 35.0%
CTGGTTTCTCATCTATTTGT+AGG - chr5_1:4236523-4236542 Msa0712400:CDS 35.0%
! AGTTCAAGCTGATTTAGTTC+AGG + chr5_1:4236606-4236625 None:intergenic 35.0%
TCAATGTCACAACAATCTAC+AGG + chr5_1:4236639-4236658 None:intergenic 35.0%
GTAGATTGTTGTGACATTGA+AGG - chr5_1:4236639-4236658 Msa0712400:CDS 35.0%
TGTAACATTCTTCTTTGACG+GGG + chr5_1:4236979-4236998 None:intergenic 35.0%
! TTCTTTGGCTTCATTTGTGA+TGG + chr5_1:4237011-4237030 None:intergenic 35.0%
TCATTACCACCAACATTATC+TGG - chr5_1:4237171-4237190 Msa0712400:CDS 35.0%
AACTTGATGTCAATGATGAC+TGG + chr5_1:4237224-4237243 None:intergenic 35.0%
! TTGACACGTCTTATTCTTGT+TGG + chr5_1:4237483-4237502 None:intergenic 35.0%
! GATAGTTCTTTGAAGATACG+AGG - chr5_1:4237609-4237628 Msa0712400:CDS 35.0%
GTTCTTACATAAGGACTCAA+AGG + chr5_1:4237726-4237745 None:intergenic 35.0%
!! TGGAAGTCTAAAAGCGCTTA+TGG - chr5_1:4235015-4235034 Msa0712400:CDS 40.0%
ATGCTAGAGTGAATATGTCG+TGG + chr5_1:4235087-4235106 None:intergenic 40.0%
ACACAATCGCAGATGAGATA+AGG - chr5_1:4235110-4235129 Msa0712400:CDS 40.0%
! TCTCTAAGAGGACATTCAAG+AGG + chr5_1:4235175-4235194 None:intergenic 40.0%
! CTTTCCAGGGTATTCAAAGA+AGG - chr5_1:4235196-4235215 Msa0712400:CDS 40.0%
TTCACCTTCTTTGAATACCC+TGG + chr5_1:4235203-4235222 None:intergenic 40.0%
AGAATGCTTGGAGAGAAGAT+AGG - chr5_1:4235515-4235534 Msa0712400:CDS 40.0%
! GAAGCACTCAAGGAGAAAAA+AGG - chr5_1:4235550-4235569 Msa0712400:CDS 40.0%
GATCCAAAGATTACCAAGTG+AGG - chr5_1:4235713-4235732 Msa0712400:intron 40.0%
! TCTCCTCACTTGGTAATCTT+TGG + chr5_1:4235719-4235738 None:intergenic 40.0%
TTACCAAGTGAGGAGAAGAT+TGG - chr5_1:4235723-4235742 Msa0712400:CDS 40.0%
TACCAAGTGAGGAGAAGATT+GGG - chr5_1:4235724-4235743 Msa0712400:CDS 40.0%
! TAGGACAAAGTTTTGCTCTG+AGG - chr5_1:4235779-4235798 Msa0712400:CDS 40.0%
!!! TTTGCTCTGAGGCATTTTCT+TGG - chr5_1:4235790-4235809 Msa0712400:CDS 40.0%
!!! TTGCTCTGAGGCATTTTCTT+GGG - chr5_1:4235791-4235810 Msa0712400:CDS 40.0%
GAGAACAAAATCTGTCTCCT+TGG + chr5_1:4235982-4236001 None:intergenic 40.0%
TTTGTTCTCTATACCTGTGG+TGG - chr5_1:4235993-4236012 Msa0712400:CDS 40.0%
GGTTGATCTCATGCTTCAAA+TGG - chr5_1:4236014-4236033 Msa0712400:CDS 40.0%
GGGTTAATCTCTTCACCAAT+TGG + chr5_1:4236225-4236244 None:intergenic 40.0%
! TCTAGCTCAGTTAAAACCTC+AGG + chr5_1:4236264-4236283 None:intergenic 40.0%
AAAGCCGATGCATATAGCTT+TGG - chr5_1:4236324-4236343 Msa0712400:CDS 40.0%
! GACAGGGAAAATTCCTTTTG+AGG - chr5_1:4236368-4236387 Msa0712400:CDS 40.0%
!!! AGATATTTTGGCGAGCGATA+TGG + chr5_1:4236456-4236475 None:intergenic 40.0%
TGTCACAACAATCTACAGGT+GGG + chr5_1:4236635-4236654 None:intergenic 40.0%
ATGTCACAACAATCTACAGG+TGG + chr5_1:4236636-4236655 None:intergenic 40.0%
! TCAAGAGTTTTCCTATGGAG+AGG - chr5_1:4236670-4236689 Msa0712400:CDS 40.0%
CATCTTCACAAACTGTGCTT+TGG + chr5_1:4236878-4236897 None:intergenic 40.0%
ATGAAGCCAAAGAAAGACCA+AGG - chr5_1:4237017-4237036 Msa0712400:CDS 40.0%
! AACTGACCTTGGTCTTTCTT+TGG + chr5_1:4237026-4237045 None:intergenic 40.0%
TACCACCAACATTATCTGGT+CGG - chr5_1:4237175-4237194 Msa0712400:CDS 40.0%
TCGGGTTTCAAGGACAAATA+AGG - chr5_1:4237194-4237213 Msa0712400:CDS 40.0%
! TTTGGAGGCCTTCTTTTAAC+TGG + chr5_1:4237255-4237274 None:intergenic 40.0%
!!! TCAGATTCTGAGACTTTTGG+AGG + chr5_1:4237270-4237289 None:intergenic 40.0%
!!! CTCTCAGATTCTGAGACTTT+TGG + chr5_1:4237273-4237292 None:intergenic 40.0%
CAAAAGTCTCAGAATCTGAG+AGG - chr5_1:4237271-4237290 Msa0712400:CDS 40.0%
GAGACCAATCACCATTTAAC+AGG - chr5_1:4237412-4237431 Msa0712400:CDS 40.0%
GTACTAACAACAGCATTGTG+AGG - chr5_1:4237505-4237524 Msa0712400:CDS 40.0%
AGCATTGTGAGGATGCAAAA+AGG - chr5_1:4237516-4237535 Msa0712400:CDS 40.0%
GCATTGTGAGGATGCAAAAA+GGG - chr5_1:4237517-4237536 Msa0712400:CDS 40.0%
TATGACCTTGAAGGACTCAT+TGG + chr5_1:4237558-4237577 None:intergenic 40.0%
ATGCTGCCATGTTTCAGATA+TGG - chr5_1:4237587-4237606 Msa0712400:CDS 40.0%
CTCAAAGGACTCAATGGAAA+TGG + chr5_1:4237645-4237664 None:intergenic 40.0%
TATGGACTCAAAGGACTCAA+TGG + chr5_1:4237651-4237670 None:intergenic 40.0%
TTGCTCACATATGGACTCAA+AGG + chr5_1:4237660-4237679 None:intergenic 40.0%
GTCCATATGTGAGCAATCAA+AGG - chr5_1:4237664-4237683 Msa0712400:CDS 40.0%
GTCCTTTGATTGCTCACATA+TGG + chr5_1:4237669-4237688 None:intergenic 40.0%
AGCAATCAAAGGACTAGAAG+AGG - chr5_1:4237675-4237694 Msa0712400:CDS 40.0%
TCCTCTTAGAGAGCTTTCCA+GGG - chr5_1:4235183-4235202 Msa0712400:CDS 45.0%
!! CTTGCTTTGCTATTTCCCTG+CGG - chr5_1:4235318-4235337 Msa0712400:CDS 45.0%
ATTGAAGCGATCGAGAATGC+TGG - chr5_1:4235343-4235362 Msa0712400:CDS 45.0%
TTGAAGCGATCGAGAATGCT+GGG - chr5_1:4235344-4235363 Msa0712400:CDS 45.0%
GCAAGCAATTGGAGAATGCT+TGG - chr5_1:4235503-4235522 Msa0712400:CDS 45.0%
ATGCTTGGAGAGAAGATAGG+TGG - chr5_1:4235518-4235537 Msa0712400:CDS 45.0%
TTCCCAATCTTCTCCTCACT+TGG + chr5_1:4235729-4235748 None:intergenic 45.0%
AAGTGAGGAGAAGATTGGGA+AGG - chr5_1:4235728-4235747 Msa0712400:CDS 45.0%
AGTGAGGAGAAGATTGGGAA+GGG - chr5_1:4235729-4235748 Msa0712400:CDS 45.0%
!!! TGCTCTGAGGCATTTTCTTG+GGG - chr5_1:4235792-4235811 Msa0712400:CDS 45.0%
!! TGTGACGGAGTTGATGAACA+AGG - chr5_1:4235924-4235943 Msa0712400:CDS 45.0%
TGAGAGAGAATTGTGGTCCA+AGG - chr5_1:4235962-4235981 Msa0712400:CDS 45.0%
ATCTCATGCTTCAAATGGCC+AGG - chr5_1:4236019-4236038 Msa0712400:CDS 45.0%
TCTCATGCTTCAAATGGCCA+GGG - chr5_1:4236020-4236039 Msa0712400:CDS 45.0%
TAGTGAGTCTGAGCCAACAA+AGG - chr5_1:4236818-4236837 Msa0712400:CDS 45.0%
ACAGTAGCCACATCCTTTGT+TGG + chr5_1:4236834-4236853 None:intergenic 45.0%
ACCACCAACATTATCTGGTC+GGG - chr5_1:4237176-4237195 Msa0712400:CDS 45.0%
GAAACCCGACCAGATAATGT+TGG + chr5_1:4237183-4237202 None:intergenic 45.0%
CATTATCTGGTCGGGTTTCA+AGG - chr5_1:4237184-4237203 Msa0712400:CDS 45.0%
CTTCTGGTGTATGACCTTGA+AGG + chr5_1:4237567-4237586 None:intergenic 45.0%
TGAAACATGGCAGCATCTTC+TGG + chr5_1:4237583-4237602 None:intergenic 45.0%
CTCAAAGGGCTCAATGCTAA+TGG + chr5_1:4237711-4237730 None:intergenic 45.0%
CAAGAACCTCACCAATGTGC+CGG + chr5_1:4234997-4235016 None:intergenic 50.0%
GTCCTCTTAGAGAGCTTTCC+AGG - chr5_1:4235182-4235201 Msa0712400:CDS 50.0%
ACCCTGGAAAGCTCTCTAAG+AGG + chr5_1:4235187-4235206 None:intergenic 50.0%
CGATCGCTTCAATAACCGCA+GGG + chr5_1:4235336-4235355 None:intergenic 50.0%
TCGATCGCTTCAATAACCGC+AGG + chr5_1:4235337-4235356 None:intergenic 50.0%
TGAAGCGATCGAGAATGCTG+GGG - chr5_1:4235345-4235364 Msa0712400:CDS 50.0%
AGAATGCTGGGGAGATGTCA+GGG - chr5_1:4235356-4235375 Msa0712400:CDS 50.0%
CTTGGAGAGAAGATAGGTGG+AGG - chr5_1:4235521-4235540 Msa0712400:CDS 50.0%
!! GAGGCTCATAGAAGCACTCA+AGG - chr5_1:4235540-4235559 Msa0712400:CDS 50.0%
GTGAGGAGAAGATTGGGAAG+GGG - chr5_1:4235730-4235749 Msa0712400:CDS 50.0%
TGAGGAGAAGATTGGGAAGG+GGG - chr5_1:4235731-4235750 Msa0712400:CDS 50.0%
GGGAAGGGGGAAGGAATTTA+AGG - chr5_1:4235744-4235763 Msa0712400:CDS 50.0%
AGCATGAGATCAACCACCAC+AGG + chr5_1:4236009-4236028 None:intergenic 50.0%
CTCATGCTTCAAATGGCCAG+GGG - chr5_1:4236021-4236040 Msa0712400:CDS 50.0%
GTGGAGATATTCAATGCCCC+TGG + chr5_1:4236040-4236059 None:intergenic 50.0%
GGCGAGCGATATGGAAACAA+AGG + chr5_1:4236447-4236466 None:intergenic 50.0%
GTCTGAGCCAACAAAGGATG+TGG - chr5_1:4236824-4236843 Msa0712400:CDS 50.0%
ACCCGACCAGATAATGTTGG+TGG + chr5_1:4237180-4237199 None:intergenic 50.0%
GACCTTGAAGGACTCATTGG+CGG + chr5_1:4237555-4237574 None:intergenic 50.0%
ATGGAACAGCTCCGGCACAT+TGG - chr5_1:4234983-4235002 Msa0712400:CDS 55.0%
CGGCACATTGGTGAGGTTCT+TGG - chr5_1:4234995-4235014 Msa0712400:CDS 55.0%
GAGAATGCTGGGGAGATGTC+AGG - chr5_1:4235355-4235374 Msa0712400:CDS 55.0%
GGAGATGTCAGGGTTGGATC+AGG - chr5_1:4235366-4235385 Msa0712400:CDS 55.0%
GAGAAGATTGGGAAGGGGGA+AGG - chr5_1:4235735-4235754 Msa0712400:CDS 55.0%
GAGAAACCAGGACGCTGAGA+CGG + chr5_1:4236513-4236532 None:intergenic 55.0%
CACCGCCAATGAGTCCTTCA+AGG - chr5_1:4237550-4237569 Msa0712400:CDS 55.0%
ACAGCTCCGGCACATTGGTG+AGG - chr5_1:4234988-4235007 Msa0712400:CDS 60.0%
TGCTGGGGAGATGTCAGGGT+TGG - chr5_1:4235360-4235379 Msa0712400:CDS 60.0%
ACTGATCCGTCTCAGCGTCC+TGG - chr5_1:4236504-4236523 Msa0712400:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 4234983 4237770 4234983 ID=Msa0712400;Name=Msa0712400
chr5_1 mRNA 4234983 4237770 4234983 ID=Msa0712400-mRNA-1;Parent=Msa0712400;Name=Msa0712400-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|893
chr5_1 exon 4235715 4237770 4235715 ID=Msa0712400-mRNA-1:exon:1686;Parent=Msa0712400-mRNA-1
chr5_1 exon 4234983 4235608 4234983 ID=Msa0712400-mRNA-1:exon:1685;Parent=Msa0712400-mRNA-1
chr5_1 CDS 4235715 4237770 4235715 ID=Msa0712400-mRNA-1:cds;Parent=Msa0712400-mRNA-1
chr5_1 CDS 4234983 4235608 4234983 ID=Msa0712400-mRNA-1:cds;Parent=Msa0712400-mRNA-1
Gene Sequence

>Msa0712400

ATGGAACAGCTCCGGCACATTGGTGAGGTTCTTGGAAGTCTAAAAGCGCTTATGGTTTTAAGCGACGAAATTCAAATCAATCAACGACAATGTTGTTTGCTCCACGACATATTCACTCTAGCATTCGACACAATCGCAGATGAGATAAGGCAGAATCTGAAGCTTGAAGACAGAACTACAAAATGGAAGCCTCTTGAATGTCCTCTTAGAGAGCTTTCCAGGGTATTCAAAGAAGGTGAATTATACATTAAGCATTGTTTGGATTCAAAAGATTGGTCAGGGAAAGCACTCACACTTTCTCAGAACAGAGAATGTGTTGAGTTTCACATTCATAACTTGCTTTGCTATTTCCCTGCGGTTATTGAAGCGATCGAGAATGCTGGGGAGATGTCAGGGTTGGATCAGGATGAGAATTCGAAGAAGAAATTGATGCTTGCAAGAAAATATGATGTGGAATGGAATGATCCAAAACTTTTTCAGTGGAGATTTGGCAAACAATATTTGGTTCCAAAAGAAATTTGCAAGCAATTGGAGAATGCTTGGAGAGAAGATAGGTGGAGGCTCATAGAAGCACTCAAGGAGAAAAAAGGTTCTTCCAAACTCACTTCTTCAAAGAATGATCAACATTTTGCAGATATGTTGTTGAAGAAATTGATGAATGGTAATTCGGATAAAACTAATCACAAAAACAGTAATGATAAGGTATTGTGGCCAATTAGTGTTTTGTTAGGATCCAAAGATTACCAAGTGAGGAGAAGATTGGGAAGGGGGAAGGAATTTAAGGAAATTCAATGGTTAGGACAAAGTTTTGCTCTGAGGCATTTTCTTGGGGAGAGAGAAGCTTATGAAAATGAGATTTCCAATTTGTTATCACTTTCTCACCCAAACATATTGCAATATCTATGTGGTTTTTATGATGAAGAGAAGAAAGAGTTTTCTCTTGTGACGGAGTTGATGAACAAGGATTTGTGGACATACATGAGAGAGAATTGTGGTCCAAGGAGACAGATTTTGTTCTCTATACCTGTGGTGGTTGATCTCATGCTTCAAATGGCCAGGGGCATTGAATATCTCCACTCCAAAAACATTTACCATGGAAACCTTAATCCTTGTAATATCCTACTAAAAGCTAGAAACTCACAAGAAGGTTATTTTCAAGCAAAAGTTGTTGGATTTGGATTGTCATCTGTTGAAAATGGTGAAATTTATAATGCTTCTAGATCCTCACCAACACATAATCCAATTGGTGAAGAGATTAACCCTATAATTTGGTATGCACCTGAGGTTTTAACTGAGCTAGAACAAATGAAAAATGCTTCTACCTCTTGTAAGTACTCAGAGAAAGCCGATGCATATAGCTTTGGTATGATATGTTTTGAGTTGTTGACAGGGAAAATTCCTTTTGAGGATAATCATTTTCAAGGGATTAGAACCAACCAAAATATCAAAGCAGGAGAGAGGCCTTTGTTTCCATATCGCTCGCCAAAATATCTTGTGAGTTTGATCAAAAAATGTTGGCAAACTGATCCGTCTCAGCGTCCTGGTTTCTCATCTATTTGTAGGATTTTGCGCTACATCAAAAAGTTTCTTTCAATGAACACCGAGTATGTTTTGATCAATCCTGAACTAAATCAGCTTGAACTTCAGAACCCACCTGTAGATTGTTGTGACATTGAAGGAATTTTTCTCAAGAGTTTTCCTATGGAGAGGACATATAATATGTCTTGTGTTTCACAAATACCTTATGAAATGTTTGCTTATAAAGTTGTTGAAAAAGGGAAGATTAATAATCAGAATCTGAGTAATAATACTATTCCTACTATAGATAATAAGGGTAGTGAGTCTGAGCCAACAAAGGATGTGGCTACTGTTCGTAGCGAAGAAAGTGATGACCAAAGCACAGTTTGTGAAGATGTTGATGCATCCATAATTAAAGATCTGGTAACCTATCCAAAATCAATTTCTGATGACACACAGTCTATTTTTTATGATACCCCGTCAAAGAAGAATGTTACAATCAAGAAACCATCACAAATGAAGCCAAAGAAAGACCAAGGAACGCCTAAATTGCAAGCAACAAAATCATTACCACCAACATTATCTGGTCGGGTTTCAAGGACAAATAAGGCGAACCAGTCATCATTGACATCAAGTTCTATAAATCCAGTTAAAAGAAGGCCTCCAAAAGTCTCAGAATCTGAGAGGAAATCCAATATTAACAAAGGGAATCAATCAGCATCAGTGTCAACTTCAAGCTCAGTTAGAAAAAAAGTATATGGAGGCAATGTCTCAGATTCTAAGCCTAGTTTAAATCTGAAAACAAGAGACCAATCACCATTTAACAGGTTAAAGACAAAAAAATCACAACTAACGATGAATTCTTCTTCGAGTCCAACAAGAATAAGACGTGTCAATAGTACTAACAACAGCATTGTGAGGATGCAAAAAGGGTTCATTTCAACACCGCCAATGAGTCCTTCAAGGTCATACACCAGAAGATGCTGCCATGTTTCAGATATGGATAGTTCTTTGAAGATACGAGGTAGATTGTCACCATTTCCATTGAGTCCTTTGAGTCCATATGTGAGCAATCAAAGGACTAGAAGAGGAAGCTTGTCACCATTAGCATTGAGCCCTTTGAGTCCTTATGTAAGAACAACATATGGTCATGTCTCAGATTAA
Protein sequence

>Msa0712400

MEQLRHIGEVLGSLKALMVLSDEIQINQRQCCLLHDIFTLAFDTIADEIRQNLKLEDRTTKWKPLECPLRELSRVFKEGELYIKHCLDSKDWSGKALTLSQNRECVEFHIHNLLCYFPAVIEAIENAGEMSGLDQDENSKKKLMLARKYDVEWNDPKLFQWRFGKQYLVPKEICKQLENAWREDRWRLIEALKEKKGSSKLTSSKNDQHFADMLLKKLMNGNSDKTNHKNSNDKVLWPISVLLGSKDYQVRRRLGRGKEFKEIQWLGQSFALRHFLGEREAYENEISNLLSLSHPNILQYLCGFYDEEKKEFSLVTELMNKDLWTYMRENCGPRRQILFSIPVVVDLMLQMARGIEYLHSKNIYHGNLNPCNILLKARNSQEGYFQAKVVGFGLSSVENGEIYNASRSSPTHNPIGEEINPIIWYAPEVLTELEQMKNASTSCKYSEKADAYSFGMICFELLTGKIPFEDNHFQGIRTNQNIKAGERPLFPYRSPKYLVSLIKKCWQTDPSQRPGFSSICRILRYIKKFLSMNTEYVLINPELNQLELQNPPVDCCDIEGIFLKSFPMERTYNMSCVSQIPYEMFAYKVVEKGKINNQNLSNNTIPTIDNKGSESEPTKDVATVRSEESDDQSTVCEDVDASIIKDLVTYPKSISDDTQSIFYDTPSKKNVTIKKPSQMKPKKDQGTPKLQATKSLPPTLSGRVSRTNKANQSSLTSSSINPVKRRPPKVSESERKSNINKGNQSASVSTSSSVRKKVYGGNVSDSKPSLNLKTRDQSPFNRLKTKKSQLTMNSSSSPTRIRRVNSTNNSIVRMQKGFISTPPMSPSRSYTRRCCHVSDMDSSLKIRGRLSPFPLSPLSPYVSNQRTRRGSLSPLALSPLSPYVRTTYGHVSD*