AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0714130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0714130 MtrunA17_Chr5g0401421 94.866 448 23 0 22 469 1 448 0.0 870
Msa0714130 MtrunA17_Chr4g0053901 66.805 482 135 10 1 469 1 470 0.0 614
Msa0714130 MtrunA17_Chr2g0323111 48.780 205 91 2 265 469 118 308 9.88e-63 206
Msa0714130 MtrunA17_Chr2g0323111 37.162 148 65 6 17 154 16 145 4.21e-16 79.3
Msa0714130 MtrunA17_Chr5g0401131 27.446 419 257 11 56 468 114 491 3.12e-44 162
Msa0714130 MtrunA17_Chr1g0159461 26.506 415 257 6 54 466 223 591 2.50e-40 152
Msa0714130 MtrunA17_Chr3g0125811 27.014 422 246 8 54 466 201 569 5.60e-40 151
Msa0714130 MtrunA17_Chr2g0329591 27.189 434 242 13 55 468 73 452 5.38e-37 141
Msa0714130 MtrunA17_Chr5g0445331 24.429 438 265 10 42 466 147 531 7.19e-35 136
Msa0714130 MtrunA17_Chr2g0319091 26.241 423 250 10 53 466 209 578 7.36e-35 137
Msa0714130 MtrunA17_Chr3g0101141 24.762 420 260 9 53 466 172 541 1.06e-34 136
Msa0714130 MtrunA17_Chr5g0447691 25.943 424 250 10 53 466 174 543 1.08e-33 133
Msa0714130 MtrunA17_Chr2g0306641 26.555 418 244 13 56 466 125 486 1.14e-33 132
Msa0714130 MtrunA17_Chr4g0076091 25.581 430 247 11 53 467 172 543 7.53e-33 130
Msa0714130 MtrunA17_Chr8g0357601 26.555 418 250 13 57 466 183 551 2.07e-32 129
Msa0714130 MtrunA17_Chr4g0031711 24.586 423 268 12 53 469 358 735 6.09e-32 129
Msa0714130 MtrunA17_Chr8g0344101 26.651 424 255 12 54 466 221 599 6.57e-31 125
Msa0714130 MtrunA17_Chr2g0290241 25.416 421 257 10 53 466 54 424 7.16e-30 120
Msa0714130 MtrunA17_Chr7g0224271 26.540 422 250 14 56 466 300 672 3.65e-29 120
Msa0714130 MtrunA17_Chr2g0328391 24.413 426 271 13 49 469 292 671 4.36e-29 120
Msa0714130 MtrunA17_Chr3g0110971 25.467 428 240 10 57 466 220 586 7.14e-29 119
Msa0714130 MtrunA17_Chr8g0376381 25.721 416 239 13 57 466 170 521 7.34e-29 119
Msa0714130 MtrunA17_Chr4g0031731 23.753 421 269 13 53 467 251 625 1.44e-28 119
Msa0714130 MtrunA17_Chr4g0031701 24.113 423 265 15 53 467 251 625 6.57e-28 117
Msa0714130 MtrunA17_Chr4g0057891 26.887 424 246 14 55 466 148 519 1.38e-27 115
Msa0714130 MtrunA17_Chr4g0031721 25.059 423 262 15 53 469 313 686 4.62e-25 108
Msa0714130 MtrunA17_Chr4g0031741 24.821 419 260 15 56 469 284 652 7.23e-25 108
Msa0714130 MtrunA17_Chr3g0085681 24.083 436 279 11 57 466 26 435 1.12e-24 105
Msa0714130 MtrunA17_Chr4g0057291 23.333 420 267 12 56 468 292 663 3.10e-24 106
Msa0714130 MtrunA17_Chr7g0236851 25.000 464 266 11 9 466 269 656 8.21e-24 104
Msa0714130 MtrunA17_Chr2g0328341 23.404 423 267 13 53 469 269 640 2.83e-23 103
Msa0714130 MtrunA17_Chr7g0238891 22.642 424 271 11 53 469 369 742 3.45e-23 103
Msa0714130 MtrunA17_Chr2g0328431 22.430 428 276 12 48 469 279 656 7.91e-22 98.6
Msa0714130 MtrunA17_Chr4g0038851 22.653 490 286 14 30 466 19 468 1.11e-21 97.4
Msa0714130 MtrunA17_Chr2g0328381 22.695 423 270 13 53 469 272 643 1.38e-21 97.8
Msa0714130 MtrunA17_Chr6g0468561 24.883 426 254 11 52 466 291 661 1.57e-21 97.8
Msa0714130 MtrunA17_Chr7g0245601 21.602 412 262 11 59 468 441 793 1.62e-21 98.2
Msa0714130 MtrunA17_Chr4g0039661 25.653 421 254 12 51 466 188 554 2.22e-21 97.1
Msa0714130 MtrunA17_Chr5g0396491 22.944 462 265 12 56 466 56 477 2.33e-21 96.7
Msa0714130 MtrunA17_Chr2g0328411 21.928 415 272 11 56 467 289 654 8.00e-21 95.9
Msa0714130 MtrunA17_Chr3g0085361 23.343 347 226 7 145 466 18 349 1.42e-20 92.8
Msa0714130 MtrunA17_Chr1g0183641 21.928 415 259 12 56 466 123 476 3.98e-19 89.7
Msa0714130 MtrunA17_Chr4g0038761 23.373 415 257 14 59 469 87 444 6.41e-19 89.0
Msa0714130 MtrunA17_Chr2g0328401 22.901 393 248 11 48 435 280 622 2.46e-18 87.8
Msa0714130 MtrunA17_Chr8g0383981 24.299 428 242 14 56 469 139 498 2.84e-17 84.3
Msa0714130 MtrunA17_Chr1g0200401 23.451 452 249 16 43 466 76 458 5.31e-16 80.1
Msa0714130 MtrunA17_Chr7g0270031 23.529 323 198 9 150 466 274 553 1.59e-14 75.9
Msa0714130 MtrunA17_Chr5g0424131 23.877 423 265 17 56 466 121 498 8.32e-14 73.6
Msa0714130 MtrunA17_Chr2g0328371 23.221 267 162 8 150 412 13 240 1.83e-13 70.1
Msa0714130 MtrunA17_Chr3g0114841 22.222 324 210 7 147 466 214 499 2.73e-13 72.0
Msa0714130 MtrunA17_Chr2g0328361 20.665 421 256 13 53 469 271 617 4.24e-13 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0714130 AT3G49950.1 56.979 437 148 9 35 468 10 409 2.63e-168 480
Msa0714130 AT5G67411.1 55.066 227 75 4 184 408 1 202 3.31e-75 235
Msa0714130 AT4G37650.1 28.188 447 271 12 35 469 123 531 1.80e-42 158
Msa0714130 AT2G04890.1 25.481 416 255 7 53 466 50 412 1.28e-34 134
Msa0714130 AT1G21450.1 26.005 423 250 9 54 466 223 592 4.15e-34 135
Msa0714130 AT5G48150.1 27.229 415 249 8 57 466 123 489 1.91e-33 132
Msa0714130 AT5G48150.4 27.229 415 249 8 57 466 123 489 1.91e-33 132
Msa0714130 AT5G48150.2 27.229 415 249 8 57 466 123 489 1.91e-33 132
Msa0714130 AT5G48150.3 27.229 415 249 8 57 466 123 489 1.91e-33 132
Msa0714130 AT1G50600.3 25.298 419 259 10 53 466 156 525 1.22e-32 130
Msa0714130 AT1G50600.1 25.298 419 259 10 53 466 227 596 1.88e-32 130
Msa0714130 AT1G50600.2 25.298 419 259 10 53 466 231 600 2.31e-32 130
Msa0714130 AT5G17490.1 25.604 414 249 6 55 466 159 515 4.96e-32 129
Msa0714130 AT3G03450.1 25.728 412 253 8 57 466 184 544 4.24e-31 126
Msa0714130 AT4G17230.1 24.648 426 257 9 53 468 155 526 1.76e-30 124
Msa0714130 AT4G17230.2 24.648 426 257 9 53 468 155 526 1.76e-30 124
Msa0714130 AT2G29065.1 26.014 419 265 12 56 469 252 630 2.68e-29 121
Msa0714130 AT3G13840.1 26.651 424 240 14 54 466 146 509 1.12e-27 115
Msa0714130 AT5G52510.1 23.041 434 257 11 52 466 264 639 1.79e-25 110
Msa0714130 AT1G14920.1 24.096 415 251 12 57 466 173 528 2.09e-25 109
Msa0714130 AT1G50420.1 24.342 456 266 14 56 466 57 478 6.62e-25 107
Msa0714130 AT2G01570.1 23.729 413 255 12 57 466 225 580 8.63e-25 107
Msa0714130 AT2G37650.2 23.934 422 264 14 53 468 332 702 2.46e-24 107
Msa0714130 AT2G37650.1 23.934 422 264 14 53 468 344 714 2.92e-24 106
Msa0714130 AT3G46600.2 22.931 423 270 9 53 469 1 373 2.12e-22 99.0
Msa0714130 AT1G66350.1 23.002 413 252 8 57 466 156 505 3.08e-22 99.8
Msa0714130 AT3G46600.1 23.239 426 265 9 53 469 209 581 8.98e-22 99.0
Msa0714130 AT5G59450.1 23.394 436 280 10 41 469 212 600 9.49e-22 99.0
Msa0714130 AT3G46600.3 23.005 426 266 9 53 469 177 549 1.30e-21 98.2
Msa0714130 AT1G55580.1 23.690 439 275 12 53 466 41 444 2.22e-21 96.7
Msa0714130 AT1G07520.2 24.424 434 252 17 56 469 154 531 2.48e-21 97.4
Msa0714130 AT1G07520.4 24.424 434 252 17 56 469 154 531 2.48e-21 97.4
Msa0714130 AT1G07520.1 24.424 434 252 17 56 469 318 695 3.41e-21 97.4
Msa0714130 AT1G07520.3 24.424 434 252 17 56 469 319 696 3.73e-21 97.1
Msa0714130 AT1G07530.1 20.665 421 284 10 53 469 393 767 1.83e-19 92.0
Msa0714130 AT1G63100.2 22.951 427 263 13 56 466 277 653 2.41e-19 91.7
Msa0714130 AT2G29060.1 22.642 424 272 12 53 468 318 693 2.62e-19 91.7
Msa0714130 AT2G29060.2 22.642 424 272 12 53 468 281 656 2.85e-19 91.3
Msa0714130 AT1G63100.1 22.951 427 263 13 56 466 252 628 3.50e-19 90.9
Msa0714130 AT5G66770.1 25.342 292 179 7 147 435 294 549 2.75e-17 85.1
Msa0714130 AT5G41920.1 22.782 417 256 14 56 466 43 399 3.33e-17 84.0
Msa0714130 AT3G50650.1 24.706 340 190 9 147 466 248 541 3.17e-13 72.4
Msa0714130 AT4G08250.1 24.706 425 251 19 56 466 107 476 7.37e-11 64.7

Find 77 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTTTCTTACGACGAATT+AGG 0.219981 5_1:+6730257 Msa0714130:CDS
CGACGAATTAGGTTCAAAAT+TGG 0.230348 5_1:+6730268 Msa0714130:CDS
AATCCGCGTTTAATTATCTT+TGG 0.234805 5_1:+6730636 Msa0714130:CDS
GCAGCTGTTAGCTTGATTAA+AGG 0.266223 5_1:-6730179 None:intergenic
TTTCAGTGAGGATTCGGTTT+TGG 0.295734 5_1:+6730844 Msa0714130:CDS
TAAAACAAGAAACGTGATTA+TGG 0.317994 5_1:+6730301 Msa0714130:CDS
AAGTGTAGTAGAGGGTTATT+AGG 0.328746 5_1:-6729696 None:intergenic
ATGAGCTTCTTGTGCTTACT+TGG 0.341746 5_1:+6730921 Msa0714130:CDS
AATGTAAATAGAGTTTCTTC+AGG 0.361286 5_1:-6730458 None:intergenic
AGATTTAACATCAAGTAGTT+TGG 0.367518 5_1:+6730601 Msa0714130:CDS
AGATCAAGCATTGGTGGAAC+TGG 0.372508 5_1:-6730239 None:intergenic
GCATTGGTGGAACTGGAATA+TGG 0.373190 5_1:-6730232 None:intergenic
CACTAGCACAACAAATCCTT+TGG 0.378101 5_1:+6729787 Msa0714130:CDS
GCTTCCTGCTAGGCGTTGAT+TGG 0.395425 5_1:-6729844 None:intergenic
TTAACACCTTGGCATCGTTT+CGG 0.442385 5_1:+6730020 Msa0714130:CDS
TCCACCAAGACGACTATTGT+TGG 0.444785 5_1:-6730569 None:intergenic
TCTCATTCGTTGCTCCCACT+TGG 0.447629 5_1:-6730798 None:intergenic
ATGATAGTTATAACTATTTG+CGG 0.450034 5_1:-6729937 None:intergenic
AGTTTGTTTCAGTGAGGATT+CGG 0.453807 5_1:+6730838 Msa0714130:CDS
GAACAAGGACTAATAACTAC+AGG 0.460908 5_1:-6729660 None:intergenic
TAATAAATCTCATGAAGCAT+TGG 0.465324 5_1:+6730403 Msa0714130:CDS
CTGAGGGTGTTGTGTTGTTA+AGG 0.465694 5_1:-6729812 None:intergenic
AAGTGTGTGGTTTGGTGAAA+AGG 0.471718 5_1:-6729606 None:intergenic
TAGTAGAGGGTTATTAGGTG+TGG 0.478477 5_1:-6729691 None:intergenic
GGTATGAAGCTGAAATTTGT+TGG 0.481427 5_1:+6730714 Msa0714130:CDS
GCAATTCTAGAAGCTACTAA+AGG 0.483791 5_1:+6730062 Msa0714130:CDS
GTCTCCAATCAACGCCTAGC+AGG 0.489729 5_1:+6729840 Msa0714130:CDS
GAGTAACTGCTCCATGCAAT+TGG 0.490828 5_1:-6729727 None:intergenic
ATGTTATGTTTGCTTCTGCT+TGG 0.495765 5_1:+6730954 Msa0714130:CDS
AGAAAACTCGTGTGTTTGTA+TGG 0.500579 5_1:-6729970 None:intergenic
CTTCTTGTGCTTACTTGGAA+AGG 0.503343 5_1:+6730926 Msa0714130:CDS
TTCTGGAATATTGCAACTAC+AGG 0.513561 5_1:-6729901 None:intergenic
TGATGAACATGCAGCTGGAT+GGG 0.514109 5_1:+6730883 Msa0714130:CDS
ATTGTTGCTTTCAATTGCAC+TGG 0.516587 5_1:-6729760 None:intergenic
GAGAATGTGATAGCATATGA+AGG 0.519545 5_1:+6730743 Msa0714130:CDS
AGATCCAACAATAGTCGTCT+TGG 0.519549 5_1:+6730565 Msa0714130:CDS
TCCAACAATAGTCGTCTTGG+TGG 0.526010 5_1:+6730568 Msa0714130:CDS
AATGTGTCCACCGTGTCAAA+CGG 0.536257 5_1:-6730662 None:intergenic
ACTAGCACAACAAATCCTTT+GGG 0.538819 5_1:+6729788 Msa0714130:CDS
GGTTGGAAAAGTGTGTGGTT+TGG 0.543864 5_1:-6729614 None:intergenic
CTTCAACAACGCCAATTGCA+TGG 0.549170 5_1:+6729716 Msa0714130:CDS
AGTAGAGGGTTATTAGGTGT+GGG 0.549560 5_1:-6729690 None:intergenic
ATGCTTGATGAACATGCAGC+TGG 0.552523 5_1:+6730878 Msa0714130:CDS
CGTTGTTGAAGTGTAGTAGA+GGG 0.553431 5_1:-6729704 None:intergenic
GTGGGAGCAACGAATGAGAA+AGG 0.554477 5_1:+6730802 Msa0714130:CDS
TTGATTGGAGACGTCGTCTG+AGG 0.558311 5_1:-6729829 None:intergenic
GATGAACATGCAGCTGGATG+GGG 0.559473 5_1:+6730884 Msa0714130:CDS
TGAAGCTGAAATTTGTTGGA+AGG 0.560306 5_1:+6730718 Msa0714130:CDS
GTTTCTTCAACTTATTCAGA+CGG 0.564374 5_1:+6730335 Msa0714130:CDS
TAAGAAAGATCAAGCATTGG+TGG 0.570387 5_1:-6730245 None:intergenic
CTCGAATGAAGCTTCCTGCT+AGG 0.577569 5_1:-6729854 None:intergenic
TTGATGAACATGCAGCTGGA+TGG 0.579446 5_1:+6730882 Msa0714130:CDS
TTGTGTTGTTAAGGACCCAA+AGG 0.585571 5_1:-6729803 None:intergenic
GCAATGGCGTCGATTAGTGT+AGG 0.586251 5_1:-6730137 None:intergenic
TGATTGGAGACGTCGTCTGA+GGG 0.588030 5_1:-6729828 None:intergenic
TGCCTTTGTTTACTCCCTCG+TGG 0.588128 5_1:-6730689 None:intergenic
GCGTTGTTGAAGTGTAGTAG+AGG 0.604252 5_1:-6729705 None:intergenic
TGTGTGGTTTGGTGAAAAGG+TGG 0.606992 5_1:-6729603 None:intergenic
GCTGTTAGCTTGATTAAAGG+AGG 0.610301 5_1:-6730176 None:intergenic
AACCTCATGATGATTTGCAA+TGG 0.610435 5_1:-6730153 None:intergenic
CAGCTGCAGTTAATAACTGT+AGG 0.611302 5_1:+6730195 Msa0714130:CDS
AATGTTGCACTTGCACTCGT+AGG 0.611711 5_1:-6730379 None:intergenic
CGCCATTGCAAATCATCATG+AGG 0.616800 5_1:+6730151 Msa0714130:CDS
TTGGATACCGTTTGACACGG+TGG 0.625357 5_1:+6730655 Msa0714130:CDS
TCTTTGGATACCGTTTGACA+CGG 0.625561 5_1:+6730652 Msa0714130:CDS
AGAATGTGATAGCATATGAA+GGG 0.625664 5_1:+6730744 Msa0714130:CDS
GAGGGAGTAAACAAAGGCAG+TGG 0.640189 5_1:+6730693 Msa0714130:CDS
TCAAGGAGTTTGTTTCAGTG+AGG 0.641089 5_1:+6730832 Msa0714130:CDS
GGTGTGGGAAATTCTGAACA+AGG 0.649547 5_1:-6729675 None:intergenic
GGTAGGGTTGGAAAAGTGTG+TGG 0.650079 5_1:-6729619 None:intergenic
TCGTAAGAAAGATCAAGCAT+TGG 0.656089 5_1:-6730248 None:intergenic
GAGTCGAGTCTAAAACCAAG+TGG 0.658958 5_1:+6730783 Msa0714130:CDS
AACAAGGACTAATAACTACA+GGG 0.659957 5_1:-6729659 None:intergenic
AGTCGAGTCTAAAACCAAGT+GGG 0.662079 5_1:+6730784 Msa0714130:CDS
TACCACGAGGGAGTAAACAA+AGG 0.668759 5_1:+6730687 Msa0714130:CDS
GTGTAACCGAAACGATGCCA+AGG 0.704462 5_1:-6730026 None:intergenic
TATCCAAAGATAATTAAACG+CGG 0.789557 5_1:-6730639 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATAGTTATAACTATTTG+CGG - chr5_1:6729940-6729959 None:intergenic 20.0%
!!! ATTTTATTGATTTAACACCT+TGG + chr5_1:6730009-6730028 Msa0714130:CDS 20.0%
!!! TTTGATTTTTGTTCATGGTA+GGG - chr5_1:6729638-6729657 None:intergenic 25.0%
!!! ATTTGATTTTTGTTCATGGT+AGG - chr5_1:6729639-6729658 None:intergenic 25.0%
! TAAAACAAGAAACGTGATTA+TGG + chr5_1:6730301-6730320 Msa0714130:CDS 25.0%
! TAATAAATCTCATGAAGCAT+TGG + chr5_1:6730403-6730422 Msa0714130:CDS 25.0%
! AATGTAAATAGAGTTTCTTC+AGG - chr5_1:6730461-6730480 None:intergenic 25.0%
! AGATTTAACATCAAGTAGTT+TGG + chr5_1:6730601-6730620 Msa0714130:CDS 25.0%
! TATCCAAAGATAATTAAACG+CGG - chr5_1:6730642-6730661 None:intergenic 25.0%
!! AGGGATTTGATTTTTGTTCA+TGG - chr5_1:6729643-6729662 None:intergenic 30.0%
AACAAGGACTAATAACTACA+GGG - chr5_1:6729662-6729681 None:intergenic 30.0%
GTTTCTTCAACTTATTCAGA+CGG + chr5_1:6730335-6730354 Msa0714130:CDS 30.0%
AATCCGCGTTTAATTATCTT+TGG + chr5_1:6730636-6730655 Msa0714130:CDS 30.0%
AGAATGTGATAGCATATGAA+GGG + chr5_1:6730744-6730763 Msa0714130:CDS 30.0%
! ATTTTTGTTCATGGTAGGGT+TGG - chr5_1:6729634-6729653 None:intergenic 35.0%
GAACAAGGACTAATAACTAC+AGG - chr5_1:6729663-6729682 None:intergenic 35.0%
AAGTGTAGTAGAGGGTTATT+AGG - chr5_1:6729699-6729718 None:intergenic 35.0%
!! ATTGTTGCTTTCAATTGCAC+TGG - chr5_1:6729763-6729782 None:intergenic 35.0%
ACTAGCACAACAAATCCTTT+GGG + chr5_1:6729788-6729807 Msa0714130:CDS 35.0%
!! TTCTGGAATATTGCAACTAC+AGG - chr5_1:6729904-6729923 None:intergenic 35.0%
AGAAAACTCGTGTGTTTGTA+TGG - chr5_1:6729973-6729992 None:intergenic 35.0%
! GCAATTCTAGAAGCTACTAA+AGG + chr5_1:6730062-6730081 Msa0714130:CDS 35.0%
! AACCTCATGATGATTTGCAA+TGG - chr5_1:6730156-6730175 None:intergenic 35.0%
!! TAAGAAAGATCAAGCATTGG+TGG - chr5_1:6730248-6730267 None:intergenic 35.0%
TCGTAAGAAAGATCAAGCAT+TGG - chr5_1:6730251-6730270 None:intergenic 35.0%
GATCTTTCTTACGACGAATT+AGG + chr5_1:6730257-6730276 Msa0714130:CDS 35.0%
CGACGAATTAGGTTCAAAAT+TGG + chr5_1:6730268-6730287 Msa0714130:CDS 35.0%
!!! CGATGTTTTTAGAAGCTGTA+CGG + chr5_1:6730537-6730556 Msa0714130:CDS 35.0%
GGTATGAAGCTGAAATTTGT+TGG + chr5_1:6730714-6730733 Msa0714130:CDS 35.0%
!! TGAAGCTGAAATTTGTTGGA+AGG + chr5_1:6730718-6730737 Msa0714130:CDS 35.0%
GAGAATGTGATAGCATATGA+AGG + chr5_1:6730743-6730762 Msa0714130:CDS 35.0%
! AGTTTGTTTCAGTGAGGATT+CGG + chr5_1:6730838-6730857 Msa0714130:CDS 35.0%
ATGTTATGTTTGCTTCTGCT+TGG + chr5_1:6730954-6730973 Msa0714130:CDS 35.0%
!! AAGTGTGTGGTTTGGTGAAA+AGG - chr5_1:6729609-6729628 None:intergenic 40.0%
AGTAGAGGGTTATTAGGTGT+GGG - chr5_1:6729693-6729712 None:intergenic 40.0%
TAGTAGAGGGTTATTAGGTG+TGG - chr5_1:6729694-6729713 None:intergenic 40.0%
CGTTGTTGAAGTGTAGTAGA+GGG - chr5_1:6729707-6729726 None:intergenic 40.0%
CACTAGCACAACAAATCCTT+TGG + chr5_1:6729787-6729806 Msa0714130:CDS 40.0%
! TTGTGTTGTTAAGGACCCAA+AGG - chr5_1:6729806-6729825 None:intergenic 40.0%
TTAACACCTTGGCATCGTTT+CGG + chr5_1:6730020-6730039 Msa0714130:CDS 40.0%
! GCTGTTAGCTTGATTAAAGG+AGG - chr5_1:6730179-6730198 None:intergenic 40.0%
! GCAGCTGTTAGCTTGATTAA+AGG - chr5_1:6730182-6730201 None:intergenic 40.0%
CAGCTGCAGTTAATAACTGT+AGG + chr5_1:6730195-6730214 Msa0714130:CDS 40.0%
! TTCTAAAAACATCGAGCGCA+GGG - chr5_1:6730531-6730550 None:intergenic 40.0%
AGATCCAACAATAGTCGTCT+TGG + chr5_1:6730565-6730584 Msa0714130:CDS 40.0%
TCTTTGGATACCGTTTGACA+CGG + chr5_1:6730652-6730671 Msa0714130:CDS 40.0%
! TGGACACATTTTTACCACGA+GGG + chr5_1:6730675-6730694 Msa0714130:CDS 40.0%
! AGTCGAGTCTAAAACCAAGT+GGG + chr5_1:6730784-6730803 Msa0714130:CDS 40.0%
!! ATGAGAAAGGCACGTTTTCA+AGG + chr5_1:6730815-6730834 Msa0714130:CDS 40.0%
! TCAAGGAGTTTGTTTCAGTG+AGG + chr5_1:6730832-6730851 Msa0714130:CDS 40.0%
!! TTTCAGTGAGGATTCGGTTT+TGG + chr5_1:6730844-6730863 Msa0714130:CDS 40.0%
!!! GATTCGGTTTTGGAGGTTAA+GGG + chr5_1:6730854-6730873 Msa0714130:CDS 40.0%
ATGAGCTTCTTGTGCTTACT+TGG + chr5_1:6730921-6730940 Msa0714130:CDS 40.0%
CTTCTTGTGCTTACTTGGAA+AGG + chr5_1:6730926-6730945 Msa0714130:CDS 40.0%
!! TGTGTGGTTTGGTGAAAAGG+TGG - chr5_1:6729606-6729625 None:intergenic 45.0%
! GGTTGGAAAAGTGTGTGGTT+TGG - chr5_1:6729617-6729636 None:intergenic 45.0%
GGTGTGGGAAATTCTGAACA+AGG - chr5_1:6729678-6729697 None:intergenic 45.0%
GCGTTGTTGAAGTGTAGTAG+AGG - chr5_1:6729708-6729727 None:intergenic 45.0%
CTTCAACAACGCCAATTGCA+TGG + chr5_1:6729716-6729735 Msa0714130:CDS 45.0%
GAGTAACTGCTCCATGCAAT+TGG - chr5_1:6729730-6729749 None:intergenic 45.0%
!! CTGAGGGTGTTGTGTTGTTA+AGG - chr5_1:6729815-6729834 None:intergenic 45.0%
! GCGGTTTTTGTAACTGCTTC+TGG - chr5_1:6729921-6729940 None:intergenic 45.0%
CGCCATTGCAAATCATCATG+AGG + chr5_1:6730151-6730170 Msa0714130:CDS 45.0%
! GCATTGGTGGAACTGGAATA+TGG - chr5_1:6730235-6730254 None:intergenic 45.0%
!! AGATCAAGCATTGGTGGAAC+TGG - chr5_1:6730242-6730261 None:intergenic 45.0%
AATGTTGCACTTGCACTCGT+AGG - chr5_1:6730382-6730401 None:intergenic 45.0%
! CTTCTAAAAACATCGAGCGC+AGG - chr5_1:6730532-6730551 None:intergenic 45.0%
TCCAACAATAGTCGTCTTGG+TGG + chr5_1:6730568-6730587 Msa0714130:CDS 45.0%
TCCACCAAGACGACTATTGT+TGG - chr5_1:6730572-6730591 None:intergenic 45.0%
AATGTGTCCACCGTGTCAAA+CGG - chr5_1:6730665-6730684 None:intergenic 45.0%
! GTGGACACATTTTTACCACG+AGG + chr5_1:6730674-6730693 Msa0714130:CDS 45.0%
TACCACGAGGGAGTAAACAA+AGG + chr5_1:6730687-6730706 Msa0714130:CDS 45.0%
! GAGTCGAGTCTAAAACCAAG+TGG + chr5_1:6730783-6730802 Msa0714130:CDS 45.0%
!!! GGATTCGGTTTTGGAGGTTA+AGG + chr5_1:6730853-6730872 Msa0714130:CDS 45.0%
ATGCTTGATGAACATGCAGC+TGG + chr5_1:6730878-6730897 Msa0714130:CDS 45.0%
TTGATGAACATGCAGCTGGA+TGG + chr5_1:6730882-6730901 Msa0714130:CDS 45.0%
TGATGAACATGCAGCTGGAT+GGG + chr5_1:6730883-6730902 Msa0714130:CDS 45.0%
! GGTAGGGTTGGAAAAGTGTG+TGG - chr5_1:6729622-6729641 None:intergenic 50.0%
TGATTGGAGACGTCGTCTGA+GGG - chr5_1:6729831-6729850 None:intergenic 50.0%
TTGATTGGAGACGTCGTCTG+AGG - chr5_1:6729832-6729851 None:intergenic 50.0%
CTCGAATGAAGCTTCCTGCT+AGG - chr5_1:6729857-6729876 None:intergenic 50.0%
GTGTAACCGAAACGATGCCA+AGG - chr5_1:6730029-6730048 None:intergenic 50.0%
!! GCAATGGCGTCGATTAGTGT+AGG - chr5_1:6730140-6730159 None:intergenic 50.0%
TTGGATACCGTTTGACACGG+TGG + chr5_1:6730655-6730674 Msa0714130:CDS 50.0%
! TGCCTTTGTTTACTCCCTCG+TGG - chr5_1:6730692-6730711 None:intergenic 50.0%
GAGGGAGTAAACAAAGGCAG+TGG + chr5_1:6730693-6730712 Msa0714130:CDS 50.0%
TCTCATTCGTTGCTCCCACT+TGG - chr5_1:6730801-6730820 None:intergenic 50.0%
GTGGGAGCAACGAATGAGAA+AGG + chr5_1:6730802-6730821 Msa0714130:CDS 50.0%
!! CAGTGAGGATTCGGTTTTGG+AGG + chr5_1:6730847-6730866 Msa0714130:CDS 50.0%
GATGAACATGCAGCTGGATG+GGG + chr5_1:6730884-6730903 Msa0714130:CDS 50.0%
GTCTCCAATCAACGCCTAGC+AGG + chr5_1:6729840-6729859 Msa0714130:CDS 55.0%
!! GCTTCCTGCTAGGCGTTGAT+TGG - chr5_1:6729847-6729866 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 6729579 6730988 6729579 ID=Msa0714130;Name=Msa0714130
chr5_1 mRNA 6729579 6730988 6729579 ID=Msa0714130-mRNA-1;Parent=Msa0714130;Name=Msa0714130-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|469
chr5_1 exon 6729579 6730988 6729579 ID=Msa0714130-mRNA-1:exon:2801;Parent=Msa0714130-mRNA-1
chr5_1 CDS 6729579 6730988 6729579 ID=Msa0714130-mRNA-1:cds;Parent=Msa0714130-mRNA-1
Gene Sequence

>Msa0714130

ATGACGCAATTCACATCAACATTTCCACCTTTTCACCAAACCACACACTTTTCCAACCCTACCATGAACAAAAATCAAATCCCTGTAGTTATTAGTCCTTGTTCAGAATTTCCCACACCTAATAACCCTCTACTACACTTCAACAACGCCAATTGCATGGAGCAGTTACTCATTCACTGTGCCAGTGCAATTGAAAGCAACAATGCAACACTAGCACAACAAATCCTTTGGGTCCTTAACAACACAACACCCTCAGACGACGTCTCCAATCAACGCCTAGCAGGAAGCTTCATTCGAGCTCTTACAACGCGTGCAAGAAGATCCTGTAGTTGCAATATTCCAGAAGCAGTTACAAAAACCGCAAATAGTTATAACTATCATCACAATCTCGCCATACAAACACACGAGTTTTCTGTCATTGAGTTAGCAAATTTTATTGATTTAACACCTTGGCATCGTTTCGGTTACACAGCAGCTAATTCTGCAATTCTAGAAGCTACTAAAGGATTTTCAGTTATTCACATTGTTGATTTGAGTTTAACACACTGCATGCAGATTCCTACACTAATCGACGCCATTGCAAATCATCATGAGGTTCCTCCTTTAATCAAGCTAACAGCTGCAGTTAATAACTGTAGGAATAAGAATATTAACCATATTCCAGTTCCACCAATGCTTGATCTTTCTTACGACGAATTAGGTTCAAAATTGGTTAATTTTGCTAAAACAAGAAACGTGATTATGGAATTCAAAGCTGTTTCTTCAACTTATTCAGACGGATTTGCTTCGTTGATTGAACACCTACGAGTGCAAGTGCAACATTTTAATAAATCTCATGAAGCATTGGTTATAAACTGTCACATGATGCTTCATTATATTCCTGAAGAAACTCTATTTACATTTAATGATACAAATTCAAATTTGAATTTGAATTTAAACTCTTGTTCATCCCTGCGCTCGATGTTTTTAGAAGCTGTACGGAGTTTAGATCCAACAATAGTCGTCTTGGTGGATGAAGATGCAGATTTAACATCAAGTAGTTTGGTTAGTAGACTAAAATCCGCGTTTAATTATCTTTGGATACCGTTTGACACGGTGGACACATTTTTACCACGAGGGAGTAAACAAAGGCAGTGGTATGAAGCTGAAATTTGTTGGAAGGTTGAGAATGTGATAGCATATGAAGGGATTCAGAGAGTTGAGAGAGTCGAGTCTAAAACCAAGTGGGAGCAACGAATGAGAAAGGCACGTTTTCAAGGAGTTTGTTTCAGTGAGGATTCGGTTTTGGAGGTTAAGGGTATGCTTGATGAACATGCAGCTGGATGGGGATTGAAGAAAGAAGATGAGCTTCTTGTGCTTACTTGGAAAGGACATAATGTTATGTTTGCTTCTGCTTGGTTACCTTCTTGA
Protein sequence

>Msa0714130

MTQFTSTFPPFHQTTHFSNPTMNKNQIPVVISPCSEFPTPNNPLLHFNNANCMEQLLIHCASAIESNNATLAQQILWVLNNTTPSDDVSNQRLAGSFIRALTTRARRSCSCNIPEAVTKTANSYNYHHNLAIQTHEFSVIELANFIDLTPWHRFGYTAANSAILEATKGFSVIHIVDLSLTHCMQIPTLIDAIANHHEVPPLIKLTAAVNNCRNKNINHIPVPPMLDLSYDELGSKLVNFAKTRNVIMEFKAVSSTYSDGFASLIEHLRVQVQHFNKSHEALVINCHMMLHYIPEETLFTFNDTNSNLNLNLNSCSSLRSMFLEAVRSLDPTIVVLVDEDADLTSSSLVSRLKSAFNYLWIPFDTVDTFLPRGSKQRQWYEAEICWKVENVIAYEGIQRVERVESKTKWEQRMRKARFQGVCFSEDSVLEVKGMLDEHAAGWGLKKEDELLVLTWKGHNVMFASAWLPS*