AlfalfaGEDB | Msa0714130

M. sativa cultivar zhongmu-4 / Msa0714130

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF03514.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0714130	XP_003611618.1	94.866	448	23	0	22	469	1	448	0.0	870

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0714130	sp\|Q9SN22\|SCL32_ARATH	56.979	437	148	9	35	468	10	409	2.58e-167	480

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0714130	G7K3V6	94.866	448	23	0	22	469	1	448	0.0	870

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TFs/TRs:

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Gene ID	Type	Classification
Msa0714130	TF	GRAS

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0714130	MtrunA17_Chr5g0401421	94.866	448	23	0	22	469	1	448	0.0	870
Msa0714130	MtrunA17_Chr4g0053901	66.805	482	135	10	1	469	1	470	0.0	614
Msa0714130	MtrunA17_Chr2g0323111	48.780	205	91	2	265	469	118	308	9.88e-63	206
Msa0714130	MtrunA17_Chr2g0323111	37.162	148	65	6	17	154	16	145	4.21e-16	79.3
Msa0714130	MtrunA17_Chr5g0401131	27.446	419	257	11	56	468	114	491	3.12e-44	162
Msa0714130	MtrunA17_Chr1g0159461	26.506	415	257	6	54	466	223	591	2.50e-40	152
Msa0714130	MtrunA17_Chr3g0125811	27.014	422	246	8	54	466	201	569	5.60e-40	151
Msa0714130	MtrunA17_Chr2g0329591	27.189	434	242	13	55	468	73	452	5.38e-37	141
Msa0714130	MtrunA17_Chr5g0445331	24.429	438	265	10	42	466	147	531	7.19e-35	136
Msa0714130	MtrunA17_Chr2g0319091	26.241	423	250	10	53	466	209	578	7.36e-35	137

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0714130	AT3G49950.1	56.979	437	148	9	35	468	10	409	2.63e-168	480
Msa0714130	AT5G67411.1	55.066	227	75	4	184	408	1	202	3.31e-75	235
Msa0714130	AT4G37650.1	28.188	447	271	12	35	469	123	531	1.80e-42	158
Msa0714130	AT2G04890.1	25.481	416	255	7	53	466	50	412	1.28e-34	134
Msa0714130	AT1G21450.1	26.005	423	250	9	54	466	223	592	4.15e-34	135
Msa0714130	AT5G48150.1	27.229	415	249	8	57	466	123	489	1.91e-33	132
Msa0714130	AT5G48150.4	27.229	415	249	8	57	466	123	489	1.91e-33	132
Msa0714130	AT5G48150.2	27.229	415	249	8	57	466	123	489	1.91e-33	132
Msa0714130	AT5G48150.3	27.229	415	249	8	57	466	123	489	1.91e-33	132
Msa0714130	AT1G50600.3	25.298	419	259	10	53	466	156	525	1.22e-32	130

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Find 77 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGGACTAATAACTACA+GGG	0.659957	5_1:-6729659	None:intergenic
AACCTCATGATGATTTGCAA+TGG	0.610435	5_1:-6730153	None:intergenic
AAGTGTAGTAGAGGGTTATT+AGG	0.328746	5_1:-6729696	None:intergenic
AAGTGTGTGGTTTGGTGAAA+AGG	0.471718	5_1:-6729606	None:intergenic
AATCCGCGTTTAATTATCTT+TGG	0.234805	5_1:+6730636	Msa0714130:CDS
AATGTAAATAGAGTTTCTTC+AGG	0.361286	5_1:-6730458	None:intergenic
AATGTGTCCACCGTGTCAAA+CGG	0.536257	5_1:-6730662	None:intergenic
AATGTTGCACTTGCACTCGT+AGG	0.611711	5_1:-6730379	None:intergenic
ACTAGCACAACAAATCCTTT+GGG	0.538819	5_1:+6729788	Msa0714130:CDS
AGAAAACTCGTGTGTTTGTA+TGG	0.500579	5_1:-6729970	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAAGGACTAATAACTACA+GGG	-	chr5_1:6729662-6729681	None:intergenic	30.0%
GTTTCTTCAACTTATTCAGA+CGG	+	chr5_1:6730335-6730354	Msa0714130:CDS	30.0%
AATCCGCGTTTAATTATCTT+TGG	+	chr5_1:6730636-6730655	Msa0714130:CDS	30.0%
AGAATGTGATAGCATATGAA+GGG	+	chr5_1:6730744-6730763	Msa0714130:CDS	30.0%
GAACAAGGACTAATAACTAC+AGG	-	chr5_1:6729663-6729682	None:intergenic	35.0%
AAGTGTAGTAGAGGGTTATT+AGG	-	chr5_1:6729699-6729718	None:intergenic	35.0%
ACTAGCACAACAAATCCTTT+GGG	+	chr5_1:6729788-6729807	Msa0714130:CDS	35.0%
AGAAAACTCGTGTGTTTGTA+TGG	-	chr5_1:6729973-6729992	None:intergenic	35.0%
TCGTAAGAAAGATCAAGCAT+TGG	-	chr5_1:6730251-6730270	None:intergenic	35.0%
GATCTTTCTTACGACGAATT+AGG	+	chr5_1:6730257-6730276	Msa0714130:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	6729579	6730988	6729579	ID=Msa0714130;Name=Msa0714130
chr5_1	mRNA	6729579	6730988	6729579	ID=Msa0714130-mRNA-1;Parent=Msa0714130;Name=Msa0714130-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|469
chr5_1	exon	6729579	6730988	6729579	ID=Msa0714130-mRNA-1:exon:2801;Parent=Msa0714130-mRNA-1
chr5_1	CDS	6729579	6730988	6729579	ID=Msa0714130-mRNA-1:cds;Parent=Msa0714130-mRNA-1

Gene Sequence

>Msa0714130

ATGACGCAATTCACATCAACATTTCCACCTTTTCACCAAACCACACACTTTTCCAACCCTACCATGAACAAAAATCAAATCCCTGTAGTTATTAGTCCTTGTTCAGAATTTCCCACACCTAATAACCCTCTACTACACTTCAACAACGCCAATTGCATGGAGCAGTTACTCATTCACTGTGCCAGTGCAATTGAAAGCAACAATGCAACACTAGCACAACAAATCCTTTGGGTCCTTAACAACACAACACCCTCAGACGACGTCTCCAATCAACGCCTAGCAGGAAGCTTCATTCGAGCTCTTACAACGCGTGCAAGAAGATCCTGTAGTTGCAATATTCCAGAAGCAGTTACAAAAACCGCAAATAGTTATAACTATCATCACAATCTCGCCATACAAACACACGAGTTTTCTGTCATTGAGTTAGCAAATTTTATTGATTTAACACCTTGGCATCGTTTCGGTTACACAGCAGCTAATTCTGCAATTCTAGAAGCTACTAAAGGATTTTCAGTTATTCACATTGTTGATTTGAGTTTAACACACTGCATGCAGATTCCTACACTAATCGACGCCATTGCAAATCATCATGAGGTTCCTCCTTTAATCAAGCTAACAGCTGCAGTTAATAACTGTAGGAATAAGAATATTAACCATATTCCAGTTCCACCAATGCTTGATCTTTCTTACGACGAATTAGGTTCAAAATTGGTTAATTTTGCTAAAACAAGAAACGTGATTATGGAATTCAAAGCTGTTTCTTCAACTTATTCAGACGGATTTGCTTCGTTGATTGAACACCTACGAGTGCAAGTGCAACATTTTAATAAATCTCATGAAGCATTGGTTATAAACTGTCACATGATGCTTCATTATATTCCTGAAGAAACTCTATTTACATTTAATGATACAAATTCAAATTTGAATTTGAATTTAAACTCTTGTTCATCCCTGCGCTCGATGTTTTTAGAAGCTGTACGGAGTTTAGATCCAACAATAGTCGTCTTGGTGGATGAAGATGCAGATTTAACATCAAGTAGTTTGGTTAGTAGACTAAAATCCGCGTTTAATTATCTTTGGATACCGTTTGACACGGTGGACACATTTTTACCACGAGGGAGTAAACAAAGGCAGTGGTATGAAGCTGAAATTTGTTGGAAGGTTGAGAATGTGATAGCATATGAAGGGATTCAGAGAGTTGAGAGAGTCGAGTCTAAAACCAAGTGGGAGCAACGAATGAGAAAGGCACGTTTTCAAGGAGTTTGTTTCAGTGAGGATTCGGTTTTGGAGGTTAAGGGTATGCTTGATGAACATGCAGCTGGATGGGGATTGAAGAAAGAAGATGAGCTTCTTGTGCTTACTTGGAAAGGACATAATGTTATGTTTGCTTCTGCTTGGTTACCTTCTTGA

Protein sequence

>Msa0714130

MTQFTSTFPPFHQTTHFSNPTMNKNQIPVVISPCSEFPTPNNPLLHFNNANCMEQLLIHCASAIESNNATLAQQILWVLNNTTPSDDVSNQRLAGSFIRALTTRARRSCSCNIPEAVTKTANSYNYHHNLAIQTHEFSVIELANFIDLTPWHRFGYTAANSAILEATKGFSVIHIVDLSLTHCMQIPTLIDAIANHHEVPPLIKLTAAVNNCRNKNINHIPVPPMLDLSYDELGSKLVNFAKTRNVIMEFKAVSSTYSDGFASLIEHLRVQVQHFNKSHEALVINCHMMLHYIPEETLFTFNDTNSNLNLNLNSCSSLRSMFLEAVRSLDPTIVVLVDEDADLTSSSLVSRLKSAFNYLWIPFDTVDTFLPRGSKQRQWYEAEICWKVENVIAYEGIQRVERVESKTKWEQRMRKARFQGVCFSEDSVLEVKGMLDEHAAGWGLKKEDELLVLTWKGHNVMFASAWLPS*