AlfalfaGEDB | Msa0714970

M. sativa cultivar zhongmu-4 / Msa0714970

KEGG: -
GO: -
PFAM: PF13837.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0714970	XP_003611755.1	81.890	254	21	3	1	254	1	229	1.23e-140	404

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0714970	G7K6Q6	81.890	254	21	3	1	254	1	229	5.87e-141	404

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TFs/TRs:

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Gene ID	Type	Classification
Msa0714970	TF	Trihelix

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0714970	MtrunA17_Chr5g0402321	81.890	254	21	3	1	254	1	229	1.13e-144	404
Msa0714970	MtrunA17_Chr4g0066271	45.872	109	57	1	15	123	109	215	9.60e-27	107
Msa0714970	MtrunA17_Chr7g0250201	49.020	102	49	2	20	119	43	143	6.91e-22	93.6
Msa0714970	MtrunA17_Chr1g0179531	31.111	135	74	4	23	144	133	261	6.77e-16	76.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0714970	AT3G24860.1	34.082	267	140	5	12	253	54	309	5.06e-44	151
Msa0714970	AT2G44730.1	44.348	115	54	2	10	120	51	159	1.23e-22	95.5
Msa0714970	AT3G10030.1	36.634	101	58	2	23	123	161	255	3.09e-15	75.1
Msa0714970	AT3G10030.2	36.634	101	58	2	23	123	161	255	3.42e-15	75.1
Msa0714970	AT3G54390.1	28.139	231	142	5	23	238	38	259	3.56e-15	73.9
Msa0714970	AT3G54390.2	28.139	231	142	5	23	238	54	275	4.96e-15	73.6
Msa0714970	AT3G58630.2	31.624	117	67	2	23	129	26	139	3.80e-14	71.2
Msa0714970	AT3G58630.1	31.624	117	67	2	23	129	26	139	4.51e-14	71.2

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Find 81 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGGTATTCACTCAAACG+GGG	0.751020	5_1:-7804443	Msa0714970:CDS
AACTGATGATGAAGAGGAGG+AGG	0.476758	5_1:-7804027	Msa0714970:CDS
AATGGGTTGAGGTTTCTTTG+TGG	0.544865	5_1:+7804514	None:intergenic
ACGACATTGAAGAGCGGTTT+TGG	0.311108	5_1:+7804343	None:intergenic
AGAAACCTCAACCCATTCCA+TGG	0.546082	5_1:-7804508	Msa0714970:CDS
AGAAATGGTATTCACTCAAA+CGG	0.486979	5_1:-7804445	Msa0714970:CDS
AGAAGCGGCGGAGGAAGTAG+CGG	0.578291	5_1:+7804268	None:intergenic
AGACGAAGAGGAGGGAGTGA+TGG	0.530323	5_1:-7804000	Msa0714970:CDS
AGAGAGGTTTAGATTAGAGA+TGG	0.466781	5_1:-7803892	Msa0714970:CDS
AGATGATGAAGGAAACAGAG+AGG	0.638850	5_1:-7803908	Msa0714970:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAATGGTATTCACTCAAA+CGG	-	chr5_1:7803921-7803940	Msa0714970:CDS	30.0%
GTATGATACTTGAATCTCAA+TGG	-	chr5_1:7804512-7804531	Msa0714970:CDS	30.0%
AGAAATGGTATTCACTCAAA+CGG	-	chr5_1:7803921-7803940	Msa0714970:CDS	30.0%
GTATGATACTTGAATCTCAA+TGG	-	chr5_1:7804512-7804531	Msa0714970:CDS	30.0%
TGATGAGGTTAATGGTTTCT+TGG	+	chr5_1:7803889-7803908	None:intergenic	35.0%
TGAATACCATTTCTCTTGGT+AGG	+	chr5_1:7803915-7803934	None:intergenic	35.0%
TGAGTGAATACCATTTCTCT+TGG	+	chr5_1:7803919-7803938	None:intergenic	35.0%
GAAATGGTATTCACTCAAAC+GGG	-	chr5_1:7803922-7803941	Msa0714970:CDS	35.0%
AAATGGTATTCACTCAAACG+GGG	-	chr5_1:7803923-7803942	Msa0714970:CDS	35.0%
TCTTCAATGTCGTCATAAGA+TGG	-	chr5_1:7804033-7804052	Msa0714970:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	7803812	7804576	7803812	ID=Msa0714970;Name=Msa0714970
chr5_1	mRNA	7803812	7804576	7803812	ID=Msa0714970-mRNA-1;Parent=Msa0714970;Name=Msa0714970-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|254
chr5_1	exon	7803812	7804576	7803812	ID=Msa0714970-mRNA-1:exon:3267;Parent=Msa0714970-mRNA-1
chr5_1	CDS	7803812	7804576	7803812	ID=Msa0714970-mRNA-1:cds;Parent=Msa0714970-mRNA-1

Gene Sequence

>Msa0714970

ATGGCCACCGCTTCACCAGCAGAACTCACTCTTTCCCTTCCCACAAAGAAACCTCAACCCATTCCATGGACCCACCAAGAAACCATTAACCTCATCAGAGCCTACCAAGAGAAATGGTATTCACTCAAACGGGGCCCACTCCGCGGCAGTCAATGGGAAGAAGTGGCTGTAGTCGTAGCCGCTCGTTGCGGCTACGATTACAACCACCCTTCCAAAACCGCTCTTCAATGTCGTCATAAGATGGAAAAACTCCGTCAACGCCACCGATCTGAAAAACACCGCCTCACCGCTACTTCCTCCGCCGCTTCTTCTCGATCCTGGCAGTATTTTCGTCTCATGGATGATCTTGAACGCGGTCCTTTACCTATCTCCGTCCGACCTCTTTCTCAGAAGCACCCTATCTCCGACGATAGCGACGGTGAGAGGGTGAAAAAGCATAGTGCTGCTAGGTCTGTTAGGTCAAGGAGTATTCATACTGTTCTTAACCAGAAACCAACAGTAACAAGACAACAACAACAACGTGATGAAACTGATGATGAAGAGGAGGAGGAGGAAGACGAAGAGGAGGGAGTGATGGCGAAGAGTTTGACAGCGGAGTTGAGAAGTTTTGCAGAGAGGATTGTAGGGTTGGAAAATAAGAAGATGGAGATGATGAAGGAAACAGAGAGGTTTAGATTAGAGATGGAGAATAAGAGGATTCGTATGATACTTGAATCTCAATGGAGGATTGTTGATTCTATTGGAAAGGCATTTGGGTCTTCTTAG

Protein sequence

>Msa0714970

MATASPAELTLSLPTKKPQPIPWTHQETINLIRAYQEKWYSLKRGPLRGSQWEEVAVVVAARCGYDYNHPSKTALQCRHKMEKLRQRHRSEKHRLTATSSAASSRSWQYFRLMDDLERGPLPISVRPLSQKHPISDDSDGERVKKHSAARSVRSRSIHTVLNQKPTVTRQQQQRDETDDEEEEEEDEEEGVMAKSLTAELRSFAERIVGLENKKMEMMKETERFRLEMENKRIRMILESQWRIVDSIGKAFGSS*