AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0715560

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0715560 MtrunA17_Chr5g0402931 83.708 178 24 1 1 173 1 178 5.94e-90 260
Msa0715560 MtrunA17_Chr5g0402951 61.475 122 34 5 1 117 1 114 2.94e-34 124
Msa0715560 MtrunA17_Chr5g0402961 66.355 107 27 4 1 101 1 104 1.06e-22 93.2
Msa0715560 MtrunA17_Chr1g0147881 60.000 55 19 2 121 172 265 319 1.59e-13 67.4
Msa0715560 MtrunA17_Chr7g0228941 49.180 61 28 2 118 175 52 112 1.20e-12 61.6
Msa0715560 MtrunA17_Chr5g0401821 52.727 55 23 2 121 172 202 256 5.36e-12 62.4
Msa0715560 MtrunA17_Chr4g0053281 53.571 56 23 2 120 172 268 323 6.11e-12 62.8
Msa0715560 MtrunA17_Chr4g0071521 51.724 58 25 2 118 172 245 302 9.02e-12 62.0
Msa0715560 MtrunA17_Chr1g0184691 53.571 56 23 2 120 172 295 350 1.35e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0715560 AT2G30590.1 54.386 57 23 2 120 173 315 371 1.44e-12 65.1
Msa0715560 AT1G29280.1 50.847 59 26 2 118 173 74 132 2.59e-12 63.5
Msa0715560 AT4G31550.1 56.364 55 21 2 121 172 249 303 5.14e-12 63.2
Msa0715560 AT4G31550.2 56.364 55 21 2 121 172 248 302 5.59e-12 63.2
Msa0715560 AT2G23320.1 53.571 56 23 2 120 172 242 297 1.33e-11 62.0
Msa0715560 AT4G24240.1 35.099 151 70 6 27 172 211 338 2.14e-11 61.6
Msa0715560 AT2G24570.1 56.364 55 21 2 121 172 246 300 2.20e-11 61.6
Msa0715560 AT2G23530.1 46.988 83 31 4 33 107 29 106 2.59e-11 61.6

Find 52 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGCGAAAGAATGGGAAAAT+TGG 0.216153 5_1:+8653902 Msa0715560:CDS
AACAGGGGATTTGGGGTTCT+TGG 0.229670 5_1:-8653982 None:intergenic
GTTGAGAGAAACAGGGGATT+TGG 0.244900 5_1:-8653991 None:intergenic
TTGAGAGAAACAGGGGATTT+GGG 0.251244 5_1:-8653990 None:intergenic
CGAGATGGGTTGAGAGAAAC+AGG 0.313803 5_1:-8653999 None:intergenic
TAGCATTTCTATCTTCTTGT+TGG 0.322006 5_1:+8655351 Msa0715560:three_prime_UTR
CCGAGAAAATCGCGAAAGAA+TGG 0.366084 5_1:+8653893 Msa0715560:CDS
GCTAATGATACAACAACATT+TGG 0.391220 5_1:-8655332 None:intergenic
CGCGAAAGAATGGGAAAATT+GGG 0.410502 5_1:+8653903 Msa0715560:CDS
CGATCCTCGTATTTCCTCGC+TGG 0.420110 5_1:-8655140 None:intergenic
GTGAAGGTGGCTTAGGTTTG+AGG 0.436792 5_1:-8653947 None:intergenic
AACTACACGTGGTGGAAATA+CGG 0.437577 5_1:+8654217 Msa0715560:CDS
CGAGAAAATCGCGAAAGAAT+GGG 0.438216 5_1:+8653894 Msa0715560:CDS
TTGTGTTTGTGATCTTCCAT+TGG 0.441496 5_1:-8653834 None:intergenic
GGATTTGGGGTTCTTGGAAA+CGG 0.450649 5_1:-8653976 None:intergenic
GTTGGACGTGAAGGTGGCTT+AGG 0.454037 5_1:-8653954 None:intergenic
GCTTCTTCAGTGTAATGAAT+CGG 0.476213 5_1:-8654145 None:intergenic
TGTGTTTGTGATCTTCCATT+GGG 0.476357 5_1:-8653833 None:intergenic
TTGGGGTTCTTGGAAACGGT+TGG 0.488034 5_1:-8653972 None:intergenic
TGAAGGTGGCTTAGGTTTGA+GGG 0.497200 5_1:-8653946 None:intergenic
GCAATTCACCATGCTACACT+TGG 0.505881 5_1:-8655117 None:intergenic
AACCTTGTAGATCGACGAGA+TGG 0.506141 5_1:-8654014 None:intergenic
ATACGGTCGTTATCAGATCA+AGG 0.519956 5_1:+8654234 Msa0715560:CDS
AGAGAATTTACGTGGAAAAG+AGG 0.527212 5_1:+8654176 Msa0715560:CDS
CGACTTCGCTTTCGCAGAGT+AGG 0.538883 5_1:-8653768 None:intergenic
TGAAGAGACATATCCTCTGA+AGG 0.539522 5_1:+8655227 Msa0715560:exon
ACCCATCTCGTCGATCTACA+AGG 0.540781 5_1:+8654012 Msa0715560:CDS
GATCCTCGTATTTCCTCGCT+GGG 0.560555 5_1:-8655139 None:intergenic
TTGGAAACGGTTGGACGTGA+AGG 0.565726 5_1:-8653963 None:intergenic
TGAGAGAAACAGGGGATTTG+GGG 0.572526 5_1:-8653989 None:intergenic
GAGAATTTACGTGGAAAAGA+GGG 0.576655 5_1:+8654177 Msa0715560:CDS
AGAGACATGGGTAGCACTGA+GGG 0.577196 5_1:+8655259 Msa0715560:three_prime_UTR
ATCTGAAATTGAAATCCCAA+TGG 0.584212 5_1:+8653818 Msa0715560:CDS
GAAACGGTTGGACGTGAAGG+TGG 0.592441 5_1:-8653960 None:intergenic
ATTTCAATTTCAGATTGAAG+AGG 0.596966 5_1:-8653810 None:intergenic
TACGTTACGATGACCACCGT+TGG 0.600577 5_1:-8655176 None:intergenic
TACGGTCGTTATCAGATCAA+GGG 0.616705 5_1:+8654235 Msa0715560:CDS
AGATTATTCCAAGTGTAGCA+TGG 0.624298 5_1:+8655109 Msa0715560:CDS
CTTCATCTAACTACACGTGG+TGG 0.625613 5_1:+8654209 Msa0715560:CDS
AAGAGACATGGGTAGCACTG+AGG 0.646724 5_1:+8655258 Msa0715560:three_prime_UTR
ACCTTGTAGATCGACGAGAT+GGG 0.660674 5_1:-8654013 None:intergenic
ATAAAATAGCACTTACAACA+TGG 0.661428 5_1:+8655374 Msa0715560:three_prime_UTR
ATCAGATCAAGGGCTCAACA+AGG 0.679629 5_1:+8654245 Msa0715560:CDS
AAACTTCATCTAACTACACG+TGG 0.681056 5_1:+8654206 Msa0715560:CDS
AATTAACAAGAGAATTTACG+TGG 0.685647 5_1:+8654168 Msa0715560:CDS
TCGTGCACGAGATGATCCAA+CGG 0.690601 5_1:+8655160 Msa0715560:CDS
GGTCATCGTAACGTACAATG+AGG 0.701798 5_1:+8655184 Msa0715560:CDS
TTGCCCAGCGAGGAAATACG+AGG 0.701881 5_1:+8655136 Msa0715560:CDS
AGATGGGTTGAGAGAAACAG+GGG 0.707457 5_1:-8653997 None:intergenic
GCATGGTGAATTGCCCAGCG+AGG 0.722194 5_1:+8655126 Msa0715560:CDS
TGCACGAGATGATCCAACGG+TGG 0.730962 5_1:+8655163 Msa0715560:CDS
GAGATGGGTTGAGAGAAACA+GGG 0.738727 5_1:-8653998 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTCTTTTCTT+TGG + chr5_1:8654099-8654118 Msa0715560:intron 10.0%
!!! TTTTTTTTTTTTCTTTTCTT+TGG + chr5_1:8654099-8654118 Msa0715560:intron 10.0%
!! GAAAAAAAAAAAATGAATGT+TGG - chr5_1:8654092-8654111 None:intergenic 15.0%
!!! ATATTTTGATTAACAAAATG+TGG - chr5_1:8654651-8654670 None:intergenic 15.0%
!! AAAAGTTAAAACATAACTAA+CGG - chr5_1:8655042-8655061 None:intergenic 15.0%
!! GAAAAAAAAAAAATGAATGT+TGG - chr5_1:8654092-8654111 None:intergenic 15.0%
!!! ATATTTTGATTAACAAAATG+TGG - chr5_1:8654651-8654670 None:intergenic 15.0%
!! AAAAGTTAAAACATAACTAA+CGG - chr5_1:8655042-8655061 None:intergenic 15.0%
!!! AATGAAAGATTGAATTTTGA+GGG - chr5_1:8654055-8654074 None:intergenic 20.0%
!! AAAAAATGAATGTTGGATTA+TGG - chr5_1:8654085-8654104 None:intergenic 20.0%
!!! GTAAAAGTAAAGTGTTTTTA+GGG - chr5_1:8654318-8654337 None:intergenic 20.0%
!!! AGTAAAAGTAAAGTGTTTTT+AGG - chr5_1:8654319-8654338 None:intergenic 20.0%
!! CATAATTTCAAAATAGTCAT+TGG - chr5_1:8654713-8654732 None:intergenic 20.0%
!!! TATTTGAAAGTGTTCTATAA+AGG + chr5_1:8654984-8655003 Msa0715560:intron 20.0%
!!! ACAAAAAAGAAATCAACTTT+TGG - chr5_1:8655080-8655099 None:intergenic 20.0%
!!! AATGAAAGATTGAATTTTGA+GGG - chr5_1:8654055-8654074 None:intergenic 20.0%
!! AAAAAATGAATGTTGGATTA+TGG - chr5_1:8654085-8654104 None:intergenic 20.0%
!!! GTAAAAGTAAAGTGTTTTTA+GGG - chr5_1:8654318-8654337 None:intergenic 20.0%
!!! AGTAAAAGTAAAGTGTTTTT+AGG - chr5_1:8654319-8654338 None:intergenic 20.0%
!! CATAATTTCAAAATAGTCAT+TGG - chr5_1:8654713-8654732 None:intergenic 20.0%
!!! TATTTGAAAGTGTTCTATAA+AGG + chr5_1:8654984-8655003 Msa0715560:intron 20.0%
!!! ACAAAAAAGAAATCAACTTT+TGG - chr5_1:8655080-8655099 None:intergenic 20.0%
! ATTTCAATTTCAGATTGAAG+AGG - chr5_1:8653813-8653832 None:intergenic 25.0%
!! CAATGAAAGATTGAATTTTG+AGG - chr5_1:8654056-8654075 None:intergenic 25.0%
! AATTAACAAGAGAATTTACG+TGG + chr5_1:8654168-8654187 Msa0715560:CDS 25.0%
! TAAGCAAGTAGTCAAATAAA+AGG + chr5_1:8654869-8654888 Msa0715560:intron 25.0%
! TCGAAATACTTGTAATTTCA+TGG + chr5_1:8654927-8654946 Msa0715560:intron 25.0%
! ATATGTCTCTTCAAAAACTT+GGG - chr5_1:8655220-8655239 None:intergenic 25.0%
! ATAAAATAGCACTTACAACA+TGG + chr5_1:8655374-8655393 Msa0715560:three_prime_UTR 25.0%
! ATTTCAATTTCAGATTGAAG+AGG - chr5_1:8653813-8653832 None:intergenic 25.0%
!! CAATGAAAGATTGAATTTTG+AGG - chr5_1:8654056-8654075 None:intergenic 25.0%
! AATTAACAAGAGAATTTACG+TGG + chr5_1:8654168-8654187 Msa0715560:CDS 25.0%
! TAAGCAAGTAGTCAAATAAA+AGG + chr5_1:8654869-8654888 Msa0715560:intron 25.0%
! TCGAAATACTTGTAATTTCA+TGG + chr5_1:8654927-8654946 Msa0715560:intron 25.0%
! ATATGTCTCTTCAAAAACTT+GGG - chr5_1:8655220-8655239 None:intergenic 25.0%
! ATAAAATAGCACTTACAACA+TGG + chr5_1:8655374-8655393 Msa0715560:three_prime_UTR 25.0%
! ATCTGAAATTGAAATCCCAA+TGG + chr5_1:8653818-8653837 Msa0715560:CDS 30.0%
!! TTGAATTTTGAGGGTGAAAA+TGG - chr5_1:8654046-8654065 None:intergenic 30.0%
TAACTACTCCTTAACTTTCT+GGG + chr5_1:8654284-8654303 Msa0715560:intron 30.0%
CTTGAAACTTAATCACGTTT+GGG + chr5_1:8654363-8654382 Msa0715560:intron 30.0%
GTGAAAATGCAAATGGAAAT+TGG + chr5_1:8654427-8654446 Msa0715560:intron 30.0%
!! ATGCAAATGGAAATTGGTTT+TGG + chr5_1:8654433-8654452 Msa0715560:intron 30.0%
!!! TGCAAATGGAAATTGGTTTT+GGG + chr5_1:8654434-8654453 Msa0715560:intron 30.0%
CTTATAATATTGACGCTAAG+AGG - chr5_1:8654483-8654502 None:intergenic 30.0%
TATATTACCCAGTCATGAAT+CGG + chr5_1:8654773-8654792 Msa0715560:intron 30.0%
CTATAAAGGAGAGATTAATC+TGG + chr5_1:8654998-8655017 Msa0715560:intron 30.0%
GATATGTCTCTTCAAAAACT+TGG - chr5_1:8655221-8655240 None:intergenic 30.0%
!!! AAGGAGTTTTTTAAGAGACA+TGG + chr5_1:8655246-8655265 Msa0715560:three_prime_UTR 30.0%
!!! AGGAGTTTTTTAAGAGACAT+GGG + chr5_1:8655247-8655266 Msa0715560:three_prime_UTR 30.0%
GCTAATGATACAACAACATT+TGG - chr5_1:8655335-8655354 None:intergenic 30.0%
TAGCATTTCTATCTTCTTGT+TGG + chr5_1:8655351-8655370 Msa0715560:three_prime_UTR 30.0%
! ATCTGAAATTGAAATCCCAA+TGG + chr5_1:8653818-8653837 Msa0715560:CDS 30.0%
!! TTGAATTTTGAGGGTGAAAA+TGG - chr5_1:8654046-8654065 None:intergenic 30.0%
TAACTACTCCTTAACTTTCT+GGG + chr5_1:8654284-8654303 Msa0715560:intron 30.0%
CTTGAAACTTAATCACGTTT+GGG + chr5_1:8654363-8654382 Msa0715560:intron 30.0%
GTGAAAATGCAAATGGAAAT+TGG + chr5_1:8654427-8654446 Msa0715560:intron 30.0%
!! ATGCAAATGGAAATTGGTTT+TGG + chr5_1:8654433-8654452 Msa0715560:intron 30.0%
!!! TGCAAATGGAAATTGGTTTT+GGG + chr5_1:8654434-8654453 Msa0715560:intron 30.0%
CTTATAATATTGACGCTAAG+AGG - chr5_1:8654483-8654502 None:intergenic 30.0%
TATATTACCCAGTCATGAAT+CGG + chr5_1:8654773-8654792 Msa0715560:intron 30.0%
CTATAAAGGAGAGATTAATC+TGG + chr5_1:8654998-8655017 Msa0715560:intron 30.0%
GATATGTCTCTTCAAAAACT+TGG - chr5_1:8655221-8655240 None:intergenic 30.0%
!!! AAGGAGTTTTTTAAGAGACA+TGG + chr5_1:8655246-8655265 Msa0715560:three_prime_UTR 30.0%
!!! AGGAGTTTTTTAAGAGACAT+GGG + chr5_1:8655247-8655266 Msa0715560:three_prime_UTR 30.0%
GCTAATGATACAACAACATT+TGG - chr5_1:8655335-8655354 None:intergenic 30.0%
TAGCATTTCTATCTTCTTGT+TGG + chr5_1:8655351-8655370 Msa0715560:three_prime_UTR 30.0%
TGTGTTTGTGATCTTCCATT+GGG - chr5_1:8653836-8653855 None:intergenic 35.0%
TTGTGTTTGTGATCTTCCAT+TGG - chr5_1:8653837-8653856 None:intergenic 35.0%
GCTTCTTCAGTGTAATGAAT+CGG - chr5_1:8654148-8654167 None:intergenic 35.0%
AGAGAATTTACGTGGAAAAG+AGG + chr5_1:8654176-8654195 Msa0715560:CDS 35.0%
GAGAATTTACGTGGAAAAGA+GGG + chr5_1:8654177-8654196 Msa0715560:CDS 35.0%
AAACTTCATCTAACTACACG+TGG + chr5_1:8654206-8654225 Msa0715560:CDS 35.0%
CTAACTACTCCTTAACTTTC+TGG + chr5_1:8654283-8654302 Msa0715560:intron 35.0%
TTACTTCGTGTGTGTTTGTT+TGG + chr5_1:8654334-8654353 Msa0715560:intron 35.0%
CCAAACGTGATTAAGTTTCA+AGG - chr5_1:8654365-8654384 None:intergenic 35.0%
CCTTGAAACTTAATCACGTT+TGG + chr5_1:8654362-8654381 Msa0715560:intron 35.0%
! TTTGCATTTTCACCGTGATT+TGG - chr5_1:8654420-8654439 None:intergenic 35.0%
AATCACGGTGAAAATGCAAA+TGG + chr5_1:8654420-8654439 Msa0715560:intron 35.0%
AGGATGTTTGGAAACAAGTT+TGG - chr5_1:8654463-8654482 None:intergenic 35.0%
TACAAATGACCAAAGTAGCA+TGG + chr5_1:8654576-8654595 Msa0715560:intron 35.0%
GAAAGTGAGTGGATAAACTT+TGG + chr5_1:8654798-8654817 Msa0715560:intron 35.0%
AGATTATTCCAAGTGTAGCA+TGG + chr5_1:8655109-8655128 Msa0715560:CDS 35.0%
CTCTTAAAAAACTCCTTCAG+AGG - chr5_1:8655243-8655262 None:intergenic 35.0%
TGTGTTTGTGATCTTCCATT+GGG - chr5_1:8653836-8653855 None:intergenic 35.0%
TTGTGTTTGTGATCTTCCAT+TGG - chr5_1:8653837-8653856 None:intergenic 35.0%
GCTTCTTCAGTGTAATGAAT+CGG - chr5_1:8654148-8654167 None:intergenic 35.0%
AGAGAATTTACGTGGAAAAG+AGG + chr5_1:8654176-8654195 Msa0715560:CDS 35.0%
GAGAATTTACGTGGAAAAGA+GGG + chr5_1:8654177-8654196 Msa0715560:CDS 35.0%
AAACTTCATCTAACTACACG+TGG + chr5_1:8654206-8654225 Msa0715560:CDS 35.0%
CTAACTACTCCTTAACTTTC+TGG + chr5_1:8654283-8654302 Msa0715560:intron 35.0%
TTACTTCGTGTGTGTTTGTT+TGG + chr5_1:8654334-8654353 Msa0715560:intron 35.0%
CCAAACGTGATTAAGTTTCA+AGG - chr5_1:8654365-8654384 None:intergenic 35.0%
CCTTGAAACTTAATCACGTT+TGG + chr5_1:8654362-8654381 Msa0715560:intron 35.0%
! TTTGCATTTTCACCGTGATT+TGG - chr5_1:8654420-8654439 None:intergenic 35.0%
AATCACGGTGAAAATGCAAA+TGG + chr5_1:8654420-8654439 Msa0715560:intron 35.0%
AGGATGTTTGGAAACAAGTT+TGG - chr5_1:8654463-8654482 None:intergenic 35.0%
TACAAATGACCAAAGTAGCA+TGG + chr5_1:8654576-8654595 Msa0715560:intron 35.0%
GAAAGTGAGTGGATAAACTT+TGG + chr5_1:8654798-8654817 Msa0715560:intron 35.0%
AGATTATTCCAAGTGTAGCA+TGG + chr5_1:8655109-8655128 Msa0715560:CDS 35.0%
CTCTTAAAAAACTCCTTCAG+AGG - chr5_1:8655243-8655262 None:intergenic 35.0%
! CCATTCTTTCGCGATTTTCT+CGG - chr5_1:8653896-8653915 None:intergenic 40.0%
CGAGAAAATCGCGAAAGAAT+GGG + chr5_1:8653894-8653913 Msa0715560:CDS 40.0%
TCGCGAAAGAATGGGAAAAT+TGG + chr5_1:8653902-8653921 Msa0715560:CDS 40.0%
CGCGAAAGAATGGGAAAATT+GGG + chr5_1:8653903-8653922 Msa0715560:CDS 40.0%
TTGAGAGAAACAGGGGATTT+GGG - chr5_1:8653993-8654012 None:intergenic 40.0%
! AACTACACGTGGTGGAAATA+CGG + chr5_1:8654217-8654236 Msa0715560:CDS 40.0%
ATACGGTCGTTATCAGATCA+AGG + chr5_1:8654234-8654253 Msa0715560:CDS 40.0%
TACGGTCGTTATCAGATCAA+GGG + chr5_1:8654235-8654254 Msa0715560:CDS 40.0%
ACTTGCATCCCAGAAAGTTA+AGG - chr5_1:8654295-8654314 None:intergenic 40.0%
TCGTGTGTGTTTGTTTGGTA+TGG + chr5_1:8654339-8654358 Msa0715560:intron 40.0%
! CTTCAGCTTTAGCCAAATCA+CGG + chr5_1:8654405-8654424 Msa0715560:intron 40.0%
ATTGACGCTAAGAGGATGTT+TGG - chr5_1:8654475-8654494 None:intergenic 40.0%
!! CGACTTTTACCATGCTACTT+TGG - chr5_1:8654588-8654607 None:intergenic 40.0%
! CTTTCAACCGATTCATGACT+GGG - chr5_1:8654783-8654802 None:intergenic 40.0%
! ACTTTCAACCGATTCATGAC+TGG - chr5_1:8654784-8654803 None:intergenic 40.0%
ATGAATCGGTTGAAAGTGAG+TGG + chr5_1:8654787-8654806 Msa0715560:intron 40.0%
TGAAGAGACATATCCTCTGA+AGG + chr5_1:8655227-8655246 Msa0715560:exon 40.0%
! CCATTCTTTCGCGATTTTCT+CGG - chr5_1:8653896-8653915 None:intergenic 40.0%
CGAGAAAATCGCGAAAGAAT+GGG + chr5_1:8653894-8653913 Msa0715560:CDS 40.0%
TCGCGAAAGAATGGGAAAAT+TGG + chr5_1:8653902-8653921 Msa0715560:CDS 40.0%
CGCGAAAGAATGGGAAAATT+GGG + chr5_1:8653903-8653922 Msa0715560:CDS 40.0%
TTGAGAGAAACAGGGGATTT+GGG - chr5_1:8653993-8654012 None:intergenic 40.0%
! AACTACACGTGGTGGAAATA+CGG + chr5_1:8654217-8654236 Msa0715560:CDS 40.0%
ATACGGTCGTTATCAGATCA+AGG + chr5_1:8654234-8654253 Msa0715560:CDS 40.0%
TACGGTCGTTATCAGATCAA+GGG + chr5_1:8654235-8654254 Msa0715560:CDS 40.0%
ACTTGCATCCCAGAAAGTTA+AGG - chr5_1:8654295-8654314 None:intergenic 40.0%
TCGTGTGTGTTTGTTTGGTA+TGG + chr5_1:8654339-8654358 Msa0715560:intron 40.0%
! CTTCAGCTTTAGCCAAATCA+CGG + chr5_1:8654405-8654424 Msa0715560:intron 40.0%
ATTGACGCTAAGAGGATGTT+TGG - chr5_1:8654475-8654494 None:intergenic 40.0%
!! CGACTTTTACCATGCTACTT+TGG - chr5_1:8654588-8654607 None:intergenic 40.0%
! CTTTCAACCGATTCATGACT+GGG - chr5_1:8654783-8654802 None:intergenic 40.0%
! ACTTTCAACCGATTCATGAC+TGG - chr5_1:8654784-8654803 None:intergenic 40.0%
ATGAATCGGTTGAAAGTGAG+TGG + chr5_1:8654787-8654806 Msa0715560:intron 40.0%
TGAAGAGACATATCCTCTGA+AGG + chr5_1:8655227-8655246 Msa0715560:exon 40.0%
CCGAGAAAATCGCGAAAGAA+TGG + chr5_1:8653893-8653912 Msa0715560:CDS 45.0%
TGAAGGTGGCTTAGGTTTGA+GGG - chr5_1:8653949-8653968 None:intergenic 45.0%
! GGATTTGGGGTTCTTGGAAA+CGG - chr5_1:8653979-8653998 None:intergenic 45.0%
TGAGAGAAACAGGGGATTTG+GGG - chr5_1:8653992-8654011 None:intergenic 45.0%
GTTGAGAGAAACAGGGGATT+TGG - chr5_1:8653994-8654013 None:intergenic 45.0%
!! AGATGGGTTGAGAGAAACAG+GGG - chr5_1:8654000-8654019 None:intergenic 45.0%
!! GAGATGGGTTGAGAGAAACA+GGG - chr5_1:8654001-8654020 None:intergenic 45.0%
ACCTTGTAGATCGACGAGAT+GGG - chr5_1:8654016-8654035 None:intergenic 45.0%
AACCTTGTAGATCGACGAGA+TGG - chr5_1:8654017-8654036 None:intergenic 45.0%
CTTCATCTAACTACACGTGG+TGG + chr5_1:8654209-8654228 Msa0715560:CDS 45.0%
ATCAGATCAAGGGCTCAACA+AGG + chr5_1:8654245-8654264 Msa0715560:CDS 45.0%
GATTTGGCTAAAGCTGAAGC+TGG - chr5_1:8654404-8654423 None:intergenic 45.0%
GCAATTCACCATGCTACACT+TGG - chr5_1:8655120-8655139 None:intergenic 45.0%
GGTCATCGTAACGTACAATG+AGG + chr5_1:8655184-8655203 Msa0715560:CDS 45.0%
CCGAGAAAATCGCGAAAGAA+TGG + chr5_1:8653893-8653912 Msa0715560:CDS 45.0%
TGAAGGTGGCTTAGGTTTGA+GGG - chr5_1:8653949-8653968 None:intergenic 45.0%
! GGATTTGGGGTTCTTGGAAA+CGG - chr5_1:8653979-8653998 None:intergenic 45.0%
TGAGAGAAACAGGGGATTTG+GGG - chr5_1:8653992-8654011 None:intergenic 45.0%
GTTGAGAGAAACAGGGGATT+TGG - chr5_1:8653994-8654013 None:intergenic 45.0%
!! AGATGGGTTGAGAGAAACAG+GGG - chr5_1:8654000-8654019 None:intergenic 45.0%
!! GAGATGGGTTGAGAGAAACA+GGG - chr5_1:8654001-8654020 None:intergenic 45.0%
ACCTTGTAGATCGACGAGAT+GGG - chr5_1:8654016-8654035 None:intergenic 45.0%
AACCTTGTAGATCGACGAGA+TGG - chr5_1:8654017-8654036 None:intergenic 45.0%
CTTCATCTAACTACACGTGG+TGG + chr5_1:8654209-8654228 Msa0715560:CDS 45.0%
ATCAGATCAAGGGCTCAACA+AGG + chr5_1:8654245-8654264 Msa0715560:CDS 45.0%
GATTTGGCTAAAGCTGAAGC+TGG - chr5_1:8654404-8654423 None:intergenic 45.0%
GCAATTCACCATGCTACACT+TGG - chr5_1:8655120-8655139 None:intergenic 45.0%
GGTCATCGTAACGTACAATG+AGG + chr5_1:8655184-8655203 Msa0715560:CDS 45.0%
GTGAAGGTGGCTTAGGTTTG+AGG - chr5_1:8653950-8653969 None:intergenic 50.0%
TTGGAAACGGTTGGACGTGA+AGG - chr5_1:8653966-8653985 None:intergenic 50.0%
TTGGGGTTCTTGGAAACGGT+TGG - chr5_1:8653975-8653994 None:intergenic 50.0%
! AACAGGGGATTTGGGGTTCT+TGG - chr5_1:8653985-8654004 None:intergenic 50.0%
!! CGAGATGGGTTGAGAGAAAC+AGG - chr5_1:8654002-8654021 None:intergenic 50.0%
ACCCATCTCGTCGATCTACA+AGG + chr5_1:8654012-8654031 Msa0715560:CDS 50.0%
GATCCTCGTATTTCCTCGCT+GGG - chr5_1:8655142-8655161 None:intergenic 50.0%
TCGTGCACGAGATGATCCAA+CGG + chr5_1:8655160-8655179 Msa0715560:CDS 50.0%
TACGTTACGATGACCACCGT+TGG - chr5_1:8655179-8655198 None:intergenic 50.0%
AAGAGACATGGGTAGCACTG+AGG + chr5_1:8655258-8655277 Msa0715560:three_prime_UTR 50.0%
! AGAGACATGGGTAGCACTGA+GGG + chr5_1:8655259-8655278 Msa0715560:three_prime_UTR 50.0%
GTGAAGGTGGCTTAGGTTTG+AGG - chr5_1:8653950-8653969 None:intergenic 50.0%
TTGGAAACGGTTGGACGTGA+AGG - chr5_1:8653966-8653985 None:intergenic 50.0%
TTGGGGTTCTTGGAAACGGT+TGG - chr5_1:8653975-8653994 None:intergenic 50.0%
! AACAGGGGATTTGGGGTTCT+TGG - chr5_1:8653985-8654004 None:intergenic 50.0%
!! CGAGATGGGTTGAGAGAAAC+AGG - chr5_1:8654002-8654021 None:intergenic 50.0%
ACCCATCTCGTCGATCTACA+AGG + chr5_1:8654012-8654031 Msa0715560:CDS 50.0%
GATCCTCGTATTTCCTCGCT+GGG - chr5_1:8655142-8655161 None:intergenic 50.0%
TCGTGCACGAGATGATCCAA+CGG + chr5_1:8655160-8655179 Msa0715560:CDS 50.0%
TACGTTACGATGACCACCGT+TGG - chr5_1:8655179-8655198 None:intergenic 50.0%
AAGAGACATGGGTAGCACTG+AGG + chr5_1:8655258-8655277 Msa0715560:three_prime_UTR 50.0%
! AGAGACATGGGTAGCACTGA+GGG + chr5_1:8655259-8655278 Msa0715560:three_prime_UTR 50.0%
CGACTTCGCTTTCGCAGAGT+AGG - chr5_1:8653771-8653790 None:intergenic 55.0%
GTTGGACGTGAAGGTGGCTT+AGG - chr5_1:8653957-8653976 None:intergenic 55.0%
GAAACGGTTGGACGTGAAGG+TGG - chr5_1:8653963-8653982 None:intergenic 55.0%
TTGCCCAGCGAGGAAATACG+AGG + chr5_1:8655136-8655155 Msa0715560:CDS 55.0%
CGATCCTCGTATTTCCTCGC+TGG - chr5_1:8655143-8655162 None:intergenic 55.0%
TGCACGAGATGATCCAACGG+TGG + chr5_1:8655163-8655182 Msa0715560:CDS 55.0%
CGCTTTCGCAGAGTAGGCAT+AGG - chr5_1:8653765-8653784 None:intergenic 55.0%
CGACTTCGCTTTCGCAGAGT+AGG - chr5_1:8653771-8653790 None:intergenic 55.0%
GTTGGACGTGAAGGTGGCTT+AGG - chr5_1:8653957-8653976 None:intergenic 55.0%
GAAACGGTTGGACGTGAAGG+TGG - chr5_1:8653963-8653982 None:intergenic 55.0%
TTGCCCAGCGAGGAAATACG+AGG + chr5_1:8655136-8655155 Msa0715560:CDS 55.0%
CGATCCTCGTATTTCCTCGC+TGG - chr5_1:8655143-8655162 None:intergenic 55.0%
TGCACGAGATGATCCAACGG+TGG + chr5_1:8655163-8655182 Msa0715560:CDS 55.0%
GCATGGTGAATTGCCCAGCG+AGG + chr5_1:8655126-8655145 Msa0715560:CDS 60.0%
GCATGGTGAATTGCCCAGCG+AGG + chr5_1:8655126-8655145 Msa0715560:CDS 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 8653765 8655432 8653765 ID=Msa0715560;Name=Msa0715560
chr5_1 mRNA 8653765 8655432 8653765 ID=Msa0715560-mRNA-1;Parent=Msa0715560;Name=Msa0715560-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0.5|0.33|1|1|1|3|203|175
chr5_1 exon 8653765 8654033 8653765 ID=Msa0715560-mRNA-1:exon:3548;Parent=Msa0715560-mRNA-1
chr5_1 exon 8654129 8654266 8654129 ID=Msa0715560-mRNA-1:exon:3549;Parent=Msa0715560-mRNA-1
chr5_1 exon 8655109 8655432 8655109 ID=Msa0715560-mRNA-1:exon:3550;Parent=Msa0715560-mRNA-1
chr5_1 CDS 8653765 8654033 8653765 ID=Msa0715560-mRNA-1:cds;Parent=Msa0715560-mRNA-1
chr5_1 CDS 8654129 8654266 8654129 ID=Msa0715560-mRNA-1:cds;Parent=Msa0715560-mRNA-1
chr5_1 CDS 8655109 8655229 8655109 ID=Msa0715560-mRNA-1:cds;Parent=Msa0715560-mRNA-1
chr5_1 three_prime_UTR 8655230 8655432 8655230 ID=Msa0715560-mRNA-1:three_prime_utr;Parent=Msa0715560-mRNA-1
Gene Sequence

>Msa0715560

ATGCCTACTCTGCGAAAGCGAAGTCGCAGAGAAATTGTTGAGAATCCTCTTCAATCTGAAATTGAAATCCCAATGGAAGATCACAAACACAACAAGTCTCAATACGAGCTCACAAGAGAAGAAAGAATCCGAGAAAATCGCGAAAGAATGGGAAAATTGGGTCTTTTCGACATTTCACTCTCCCTCAAACCTAAGCCACCTTCACGTCCAACCGTTTCCAAGAACCCCAAATCCCCTGTTTCTCTCAACCCATCTCGTCGATCTACAAGATTGCAGAATGTTGCTCCGATTCATTACACTGAAGAAGCAATTAACAAGAGAATTTACGTGGAAAAGAGGGTGAAGAAAACTTCATCTAACTACACGTGGTGGAAATACGGTCGTTATCAGATCAAGGGCTCAACAAGAGATTATTCCAAGTGTAGCATGGTGAATTGCCCAGCGAGGAAATACGAGGATCGTGCACGAGATGATCCAACGGTGGTCATCGTAACGTACAATGAGGAGCACACACACCCAAGTTTTTGA
Protein sequence

>Msa0715560

MPTLRKRSRREIVENPLQSEIEIPMEDHKHNKSQYELTREERIRENRERMGKLGLFDISLSLKPKPPSRPTVSKNPKSPVSLNPSRRSTRLQNVAPIHYTEEAINKRIYVEKRVKKTSSNYTWWKYGRYQIKGSTRDYSKCSMVNCPARKYEDRARDDPTVVIVTYNEEHTHPSF*