AlfalfaGEDB | Msa0717320

M. sativa cultivar zhongmu-4 / Msa0717320

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009888 GO:0009889 GO:0009987 GO:0010087 GO:0010089 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0043565 GO:0044087 GO:0044089 GO:0048518 GO:0048522 GO:0048759 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901348 GO:1901363 GO:1903338 GO:1903340 GO:1903506 GO:1905177 GO:2000112 GO:2000652 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0717320	QDX01716.1	99.432	352	2	0	1	352	1	352	0.0	737

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0717320	sp\|Q9SL41\|NAC37_ARATH	62.334	377	103	14	1	351	1	364	9.01e-151	432

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0717320	A0A6B7JGE3	99.432	352	2	0	1	352	1	352	0.0	737

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TFs/TRs:

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Gene ID	Type	Classification
Msa0717320	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0717320	MtrunA17_Chr5g0405211	98.295	352	6	0	1	352	1	352	0.0	731
Msa0717320	MtrunA17_Chr8g0372121	63.662	355	76	9	4	351	2	310	3.11e-151	428
Msa0717320	MtrunA17_Chr4g0018391	57.278	316	95	8	6	294	5	307	2.83e-114	335
Msa0717320	MtrunA17_Chr4g0056541	56.410	312	99	6	6	294	5	302	1.03e-111	330
Msa0717320	MtrunA17_Chr5g0398971	54.455	303	115	9	6	294	5	298	2.28e-105	313
Msa0717320	MtrunA17_Chr1g0200561	49.249	333	133	8	4	320	15	327	4.27e-101	302
Msa0717320	MtrunA17_Chr2g0309331	49.338	302	127	6	3	290	5	294	5.77e-91	276
Msa0717320	MtrunA17_Chr8g0346001	43.440	343	133	7	5	297	12	343	1.14e-84	263
Msa0717320	MtrunA17_Chr4g0017921	73.418	158	41	1	1	158	1	157	1.99e-84	259
Msa0717320	MtrunA17_Chr3g0091921	50.526	190	81	4	8	193	16	196	3.44e-60	196

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0717320	AT2G18060.3	62.334	377	103	14	1	351	1	364	9.16e-152	432
Msa0717320	AT2G18060.1	62.334	377	103	14	1	351	1	364	9.16e-152	432
Msa0717320	AT4G36160.1	64.545	330	87	12	1	310	1	320	1.17e-141	406
Msa0717320	AT4G36160.3	64.545	330	87	12	1	310	1	320	1.17e-141	406
Msa0717320	AT4G36160.2	64.545	330	87	12	1	310	5	324	1.17e-141	407
Msa0717320	AT5G66300.1	59.272	302	83	9	6	291	9	286	1.62e-117	342
Msa0717320	AT1G12260.1	51.170	342	110	8	6	294	5	342	7.46e-107	318
Msa0717320	AT1G62700.1	49.575	353	126	7	6	315	5	348	6.08e-106	316
Msa0717320	AT1G62700.2	49.575	353	126	7	6	315	5	348	6.08e-106	316
Msa0717320	AT1G12260.2	54.110	292	124	4	6	294	5	289	5.70e-103	307

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Find 80 sgRNAs with CRISPR-Local

Find 249 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAATAATAACCCTAGCT+CGG	0.552141	5_1:+11101332	Msa0717320:CDS
AAGAACTAACAGAGCTACCA+TGG	0.599903	5_1:+11099134	Msa0717320:CDS
AAGCACAATGTCTCTAGTAT+CGG	0.584153	5_1:+11101137	Msa0717320:CDS
AATCAGATTTCTGTCCATTA+GGG	0.308366	5_1:-11099254	None:intergenic
AATGAAGGACTTTCAAGCTG+AGG	0.644381	5_1:-11101096	None:intergenic
ACATATATAATATATATCCA+TGG	0.602366	5_1:-11101542	None:intergenic
ACTAACAGAGCTACCATGGC+TGG	0.441475	5_1:+11099138	Msa0717320:CDS
ACTCCATGATAGAAAATTAC+TGG	0.373275	5_1:-11098676	None:intergenic
AGAAGTAGACAAAATGATCA+AGG	0.501566	5_1:-11097150	None:intergenic
AGATGAAACTAATTGGGATG+AGG	0.547240	5_1:+11099202	Msa0717320:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAGTAGACAAAATGATCA+AGG	-	chr5_1:11097153-11097172	None:intergenic	30.0%
CTCTAGTAATATTTCTCATC+AGG	+	chr5_1:11097179-11097198	Msa0717320:five_prime_UTR	30.0%
TGACGTAATCATAGTAAAAC+GGG	-	chr5_1:11097850-11097869	None:intergenic	30.0%
GTGACGTAATCATAGTAAAA+CGG	-	chr5_1:11097851-11097870	None:intergenic	30.0%
CATTACTAGGTTAATAAGGA+TGG	-	chr5_1:11097876-11097895	None:intergenic	30.0%
TTATGATTCATCAACAAACG+GGG	-	chr5_1:11098112-11098131	None:intergenic	30.0%
ATAAGAAACGGACATGTATA+GGG	-	chr5_1:11098179-11098198	None:intergenic	30.0%
TATAAGAAACGGACATGTAT+AGG	-	chr5_1:11098180-11098199	None:intergenic	30.0%
ATAATCATAAGGTCATTTGG+TGG	-	chr5_1:11098427-11098446	None:intergenic	30.0%
GAGATAATCATAAGGTCATT+TGG	-	chr5_1:11098430-11098449	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	11097080	11101667	11097080	ID=Msa0717320;Name=Msa0717320
chr5_1	mRNA	11097080	11101667	11097080	ID=Msa0717320-mRNA-1;Parent=Msa0717320;Name=Msa0717320-mRNA-1;_AED=0.07;_eAED=0.07;_QI=139\|1\|0.75\|1\|0.66\|0.5\|4\|197\|352
chr5_1	exon	11097080	11097200	11097080	ID=Msa0717320-mRNA-1:exon:4551;Parent=Msa0717320-mRNA-1
chr5_1	exon	11098674	11098854	11098674	ID=Msa0717320-mRNA-1:exon:4552;Parent=Msa0717320-mRNA-1
chr5_1	exon	11099055	11099326	11099055	ID=Msa0717320-mRNA-1:exon:4553;Parent=Msa0717320-mRNA-1
chr5_1	exon	11100847	11101667	11100847	ID=Msa0717320-mRNA-1:exon:4554;Parent=Msa0717320-mRNA-1
chr5_1	five_prime_UTR	11097080	11097200	11097080	ID=Msa0717320-mRNA-1:five_prime_utr;Parent=Msa0717320-mRNA-1
chr5_1	five_prime_UTR	11098674	11098691	11098674	ID=Msa0717320-mRNA-1:five_prime_utr;Parent=Msa0717320-mRNA-1
chr5_1	CDS	11098692	11098854	11098692	ID=Msa0717320-mRNA-1:cds;Parent=Msa0717320-mRNA-1
chr5_1	CDS	11099055	11099326	11099055	ID=Msa0717320-mRNA-1:cds;Parent=Msa0717320-mRNA-1
chr5_1	CDS	11100847	11101470	11100847	ID=Msa0717320-mRNA-1:cds;Parent=Msa0717320-mRNA-1
chr5_1	three_prime_UTR	11101471	11101667	11101471	ID=Msa0717320-mRNA-1:three_prime_utr;Parent=Msa0717320-mRNA-1

Gene Sequence

>Msa0717320

ATGGAGTCCATGGAGTCATGTGTCCCACCAGGGTTTCGGTTCCACCCAACTGATGAAGAACTTGTTGGCTATTATCTCAGAAAGAAAATTGCTTCTCAGAAAATTGACCTTGATGTTATCAGAGAGATCGATCTTTATCGTATTGAACCATGGGATCTTCAAGAGAGATGCAGGATCGGGTATGAAGAGCAGAATGAGTGGTATTTTTTCAGCCATAAAGATAAAAAGTATCCAACAGGGACAAGAACTAACAGAGCTACCATGGCTGGGTTTTGGAAAGCTACAGGAAGAGATAAAGCAGTTTATGATAAGATGAAACTAATTGGGATGAGGAAGACTCTAGTTTTCTACAAAGGAAGAGCCCCTAATGGACAGAAATCTGATTGGATCATGCATGAATATAGGCTTGAATCTGATGAAAATGGACCTCCACAGGAAGAAGGATGGGTAGTGTGTAGAGCATTTAAGAAAAGAACAACAAACAGCCAAAGTACAAAAAGTACTATTGAAGGATGGGACACAAGCTACTTCTATGAAGAAGCTGCAAGTAGGGTCAACAATTCCATTGTGGATCCAATTGATCAACTAATCTCAAGGCAACCTCAAAGCTATTTATCCCAAAGTTATAACATGTGTAAGCAAGAGATAGAAGCATGCATGCATGCAGAACAATTTTTGCAGCTTCCTCAGCTTGAAAGTCCTTCATTGCCATTAGTAAAGAGGCCAAGCACAATGTCTCTAGTATCGGACAATAATAACGATGACATTAACGACCAAAACAGGTTGCTATTATTATCGAACAACAACGATAATATTACTACAAATACAGTTACTGATTGGAGAGCTTTTGATAAGTTTGTGGCTTCTCAACTGAGTCATGAAGTTGAAACTGATCAAGGAGTGTTGTTGTCAAGTTTTGGAACAAATAATAACCCTAGCTCGGACATGGCATTGATGTTACTGCAGCAGGGTAATAGGGATGAAGAGAACAAGTTAAGTCCATTTTTGAATGCAAGTTCAGATTGCAGTGATATTGGGATATGCGTATTTGAAAAATGA

Protein sequence

>Msa0717320

MESMESCVPPGFRFHPTDEELVGYYLRKKIASQKIDLDVIREIDLYRIEPWDLQERCRIGYEEQNEWYFFSHKDKKYPTGTRTNRATMAGFWKATGRDKAVYDKMKLIGMRKTLVFYKGRAPNGQKSDWIMHEYRLESDENGPPQEEGWVVCRAFKKRTTNSQSTKSTIEGWDTSYFYEEAASRVNNSIVDPIDQLISRQPQSYLSQSYNMCKQEIEACMHAEQFLQLPQLESPSLPLVKRPSTMSLVSDNNNDDINDQNRLLLLSNNNDNITTNTVTDWRAFDKFVASQLSHEVETDQGVLLSSFGTNNNPSSDMALMLLQQGNRDEENKLSPFLNASSDCSDIGICVFEK*