AlfalfaGEDB | Msa0718090

M. sativa cultivar zhongmu-4 / Msa0718090

KEGG: K08832
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0718090	XP_003612370.1	99.103	446	4	0	1	446	1	446	0.0	924

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0718090	sp\|Q45FA5\|SRPK_PHYPO	45.946	407	202	7	10	414	29	419	4.83e-115	347

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0718090	G7K189	99.103	446	4	0	1	446	1	446	0.0	924

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0718090	MtrunA17_Chr5g0407121	99.103	446	4	0	1	446	1	446	0.0	924
Msa0718090	MtrunA17_Chr8g0370321	82.287	446	75	2	1	446	1	442	0.0	743
Msa0718090	MtrunA17_Chr1g0179611	53.066	473	162	6	2	415	8	479	1.29e-174	500
Msa0718090	MtrunA17_Chr2g0320631	48.450	516	186	5	6	442	8	522	4.46e-169	486
Msa0718090	MtrunA17_Chr4g0027671	51.496	468	162	5	12	415	13	479	2.22e-168	484
Msa0718090	MtrunA17_Chr3g0139371	31.266	387	196	10	37	414	73	398	1.40e-43	157
Msa0718090	MtrunA17_Chr3g0116701	27.970	404	219	10	21	414	78	419	3.05e-38	143
Msa0718090	MtrunA17_Chr4g0058241	28.429	401	215	11	21	411	82	420	3.78e-37	140
Msa0718090	MtrunA17_Chr3g0123491	28.117	409	216	12	21	415	77	421	7.67e-35	134
Msa0718090	MtrunA17_Chr5g0437131	24.495	396	205	11	29	414	394	705	1.17e-23	103

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0718090	AT4G35500.1	73.094	446	108	4	1	443	1	437	0.0	656
Msa0718090	AT2G17530.1	75.730	445	97	4	1	443	1	436	0.0	648
Msa0718090	AT2G17530.3	75.730	445	97	4	1	443	1	436	0.0	648
Msa0718090	AT4G35500.2	70.917	447	117	5	1	443	1	438	0.0	631
Msa0718090	AT2G17530.2	79.592	343	67	3	1	341	1	342	0.0	518
Msa0718090	AT3G53030.1	49.901	505	184	8	5	442	7	509	1.62e-170	489
Msa0718090	AT3G44850.1	51.613	465	163	5	12	415	19	482	1.65e-163	472
Msa0718090	AT5G22840.1	50.753	465	172	5	4	415	11	471	2.22e-163	471
Msa0718090	AT4G32660.3	30.370	405	208	12	21	414	47	388	2.56e-42	154
Msa0718090	AT4G32660.1	30.370	405	208	12	21	414	55	396	2.69e-42	154

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Find 133 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAACGTTGATCTCGTGA+AGG	0.622147	5_1:+12260439	None:intergenic
AAATCAAGGTAGGAAAGTGA+AGG	0.604919	5_1:-12258607	Msa0718090:exon
AAATGAATCTACTGTGGAAA+AGG	0.456827	5_1:-12258654	Msa0718090:CDS
AAATGGGCAGCACCATTGCA+TGG	0.610699	5_1:-12260340	Msa0718090:CDS
AACAAAACACAAGACCCAGA+TGG	0.607452	5_1:+12261910	None:intergenic
AACTCCTCGGAAAGATGCCC+CGG	0.493756	5_1:-12259011	Msa0718090:CDS
AAGAACGTTGATCTCGTGAA+GGG	0.630667	5_1:+12260440	None:intergenic
AAGGATTACTTTGACCGGCA+TGG	0.580864	5_1:-12258884	Msa0718090:CDS
AAGGGCAGCCTGAACAAACT+CGG	0.595404	5_1:+12260458	None:intergenic
AAGTCTAGTAGAAATATCGA+CGG	0.621748	5_1:-12259955	Msa0718090:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGAGAATCACCAATGAAAT+GGG	+	chr5_1:12258322-12258341	None:intergenic	30.0%
TTTGAGAATCACCAATGAAA+TGG	+	chr5_1:12258323-12258342	None:intergenic	30.0%
AGTATAAGAAGAACATACAG+AGG	+	chr5_1:12258609-12258628	None:intergenic	30.0%
GAATTGGAATAGCTGAAAAT+TGG	-	chr5_1:12258894-12258913	Msa0718090:CDS	30.0%
AAACAATGTAACATAAGGTC+AGG	+	chr5_1:12258944-12258963	None:intergenic	30.0%
CATGTACCATTTATGTTTGT+TGG	-	chr5_1:12259021-12259040	Msa0718090:CDS	30.0%
GTACTTCCAACAAACATAAA+TGG	+	chr5_1:12259030-12259049	None:intergenic	30.0%
ATTTATGGTATCACAAGTCT+TGG	+	chr5_1:12259147-12259166	None:intergenic	30.0%
AAGACTTGTGATACCATAAA+TGG	-	chr5_1:12259146-12259165	Msa0718090:intron	30.0%
ATGGGATGAAACATAATACT+AGG	-	chr5_1:12259242-12259261	Msa0718090:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	12258220	12262087	12258220	ID=Msa0718090;Name=Msa0718090
chr5_1	mRNA	12258220	12262087	12258220	ID=Msa0718090-mRNA-1;Parent=Msa0718090;Name=Msa0718090-mRNA-1;_AED=0.02;_eAED=0.02;_QI=208\|1\|1\|1\|1\|1\|4\|390\|446
chr5_1	exon	12258220	12258930	12258220	ID=Msa0718090-mRNA-1:exon:4981;Parent=Msa0718090-mRNA-1
chr5_1	exon	12259008	12259055	12259008	ID=Msa0718090-mRNA-1:exon:4980;Parent=Msa0718090-mRNA-1
chr5_1	exon	12259726	12260514	12259726	ID=Msa0718090-mRNA-1:exon:4979;Parent=Msa0718090-mRNA-1
chr5_1	exon	12261697	12262087	12261697	ID=Msa0718090-mRNA-1:exon:4978;Parent=Msa0718090-mRNA-1
chr5_1	five_prime_UTR	12261880	12262087	12261880	ID=Msa0718090-mRNA-1:five_prime_utr;Parent=Msa0718090-mRNA-1
chr5_1	CDS	12261697	12261879	12261697	ID=Msa0718090-mRNA-1:cds;Parent=Msa0718090-mRNA-1
chr5_1	CDS	12259726	12260514	12259726	ID=Msa0718090-mRNA-1:cds;Parent=Msa0718090-mRNA-1
chr5_1	CDS	12259008	12259055	12259008	ID=Msa0718090-mRNA-1:cds;Parent=Msa0718090-mRNA-1
chr5_1	CDS	12258610	12258930	12258610	ID=Msa0718090-mRNA-1:cds;Parent=Msa0718090-mRNA-1
chr5_1	three_prime_UTR	12258220	12258609	12258220	ID=Msa0718090-mRNA-1:three_prime_utr;Parent=Msa0718090-mRNA-1

Gene Sequence

>Msa0718090

ATGTCTTGTTCTTCTTCATCAGGATCGGAGGAAGATGATGAGGGATTTGAGTCCTATCGGAAAGGTGGATACCATGCTGTTAGAGTCGCTGATCAATTCGCGGGTGGGAGGTATATTGCTCAGAGGAAACTTGGTTGGGGTCAATTCTCCACCGTTTGGCTTGCTTATGATACTACCACCTCTTCATACGTTGCTCTCAAAATCCAGAAAAGTGCGGCCGAGTTTGTTCAGGCTGCCCTTCACGAGATCAACGTTCTTTCAACCATTGCTGATGGGGACCCATCGAATTCAAAGTTTGTTGTCCAATTGATTGATAACTTTAAGCACACAGGTCCAAATGGGCAGCACCATTGCATGGTCCTTGAGTTTCTTGGTGATAGCTTGCTTCGTTTGATCAAATACAGCCATTACAAAGGTCTTCCATTGAATAAAGTTAGAGAGATTTGTAAATACATTTTGATTGGTTTGGATTACTTGCATAGTGAACTTGGTATAATCCACACAGACTTAAAGCCTGAAAACATTCTTCTATTTTCAACTATTGATCCTTCCAAAGACCCTTTTAGATCCGGACTCTCCCCAACTTTAGAACGGACTGAGGGAAACACAAACGGTGGACTTACAAGTCTTATTGAGAAAAGGCTGAAGAGAAGAGCAAAGCGTGCAGTTGCTAAAATATCGGGAAGAACAGCTTCAATTGAAGGAAGAGGAGAAGCTGCAAAGTCTAGTAGAAATATCGACGGGATTGATGTGAGATGCAAGATTGTCGATTTTGGAAATGCGTGTTGGGCTGATAACAAGTTTGCAGAAGAAATACAGACAAGACAATATAGAGCCCCTGAAGTTATACTGAAGTCTGGTTATTCCTTCTCTGTAGACATGTGGTCTTTTGCTTGCATTGCTTTCGAGCTTGCCACTGGAGATATGTTATTTACACCCAAAGGTGGTGGACAAGGTTATAGCGAGGATGAGGATCATCTTGCCTTGATGATGGAACTCCTCGGAAAGATGCCCCGGAAGATTGCAATTGGTGGAGCTCAATCCAAGGATTACTTTGACCGGCATGGTGATCTAAAAAGGATTCGTAGGCTAAAATTTTGTCCACTTGACAAATTGCTGATTGATAGATATAAATTTTCAGCGAATGATGCTCAAGAGTTTTCAGAATTTCTTTTGCCACTTTTTGATTTTGCGCCAGAAAAACGACCAACTGCACGGCAATACCTGCAACACCCCTGGCTGAACTGCAATGAATCAGCGCCAAATGAAATGAGAAATGAATCTACTGTGGAAAAGGTTAATGTTGGTATGAGCAACCTTCAAATCAAGGTAGGAAAGTGA

Protein sequence

>Msa0718090

MSCSSSSGSEEDDEGFESYRKGGYHAVRVADQFAGGRYIAQRKLGWGQFSTVWLAYDTTTSSYVALKIQKSAAEFVQAALHEINVLSTIADGDPSNSKFVVQLIDNFKHTGPNGQHHCMVLEFLGDSLLRLIKYSHYKGLPLNKVREICKYILIGLDYLHSELGIIHTDLKPENILLFSTIDPSKDPFRSGLSPTLERTEGNTNGGLTSLIEKRLKRRAKRAVAKISGRTASIEGRGEAAKSSRNIDGIDVRCKIVDFGNACWADNKFAEEIQTRQYRAPEVILKSGYSFSVDMWSFACIAFELATGDMLFTPKGGGQGYSEDEDHLALMMELLGKMPRKIAIGGAQSKDYFDRHGDLKRIRRLKFCPLDKLLIDRYKFSANDAQEFSEFLLPLFDFAPEKRPTARQYLQHPWLNCNESAPNEMRNESTVEKVNVGMSNLQIKVGK*