AlfalfaGEDB | Msa0719350

M. sativa cultivar zhongmu-4 / Msa0719350

KEGG: -
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0719350	AES95624.2	84.167	240	16	4	1	229	1	229	1.19e-123	360

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0719350	sp\|Q8S9H7\|DIV_ANTMA	41.007	139	68	5	31	159	66	200	2.21e-22	95.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0719350	G7K884	84.167	240	16	4	1	229	1	229	5.70e-124	360

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TFs/TRs:

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Gene ID	Type	Classification
Msa0719350	TF	MYB-related

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0719350	MtrunA17_Chr5g0409831	84.167	240	16	4	1	229	1	229	1.10e-127	360
Msa0719350	MtrunA17_Chr5g0409841	64.463	242	62	7	1	229	1	231	8.96e-99	287
Msa0719350	MtrunA17_Chr5g0440731	52.837	282	76	7	1	229	17	294	2.99e-86	257
Msa0719350	MtrunA17_Chr5g0440741	52.128	282	73	8	1	229	17	289	1.98e-81	245
Msa0719350	MtrunA17_Chr7g0241761	56.579	76	30	1	84	159	136	208	1.23e-22	93.6
Msa0719350	MtrunA17_Chr1g0210471	33.146	178	102	6	34	200	67	238	1.78e-22	92.0
Msa0719350	MtrunA17_Chr3g0104071	43.165	139	66	6	29	159	82	215	1.95e-22	93.2
Msa0719350	MtrunA17_Chr1g0162081	37.500	160	79	7	23	165	41	196	2.47e-22	92.8
Msa0719350	MtrunA17_Chr5g0429521	57.895	76	29	1	84	159	128	200	9.51e-22	91.3
Msa0719350	MtrunA17_Chr5g0436501	50.538	93	41	2	64	156	105	192	3.82e-21	89.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0719350	AT5G23650.1	38.547	179	90	6	37	205	61	229	3.18e-23	96.3
Msa0719350	AT2G38090.1	39.726	146	66	6	31	159	69	209	3.48e-23	95.5
Msa0719350	AT5G08520.1	37.179	156	81	4	23	165	41	192	5.14e-23	94.7
Msa0719350	AT5G58900.1	57.895	76	29	1	84	159	137	209	1.00e-22	94.0
Msa0719350	AT5G01200.1	38.514	148	68	5	31	159	71	214	3.20e-22	92.0
Msa0719350	AT3G11280.2	39.695	131	69	4	31	159	73	195	8.66e-22	90.9
Msa0719350	AT3G11280.1	39.695	131	69	4	31	159	73	195	8.66e-22	90.9
Msa0719350	AT5G04760.1	48.913	92	42	2	76	167	87	173	2.85e-21	88.6
Msa0719350	AT5G05790.2	38.346	133	74	4	31	159	71	199	1.02e-20	88.2
Msa0719350	AT5G05790.1	38.346	133	74	4	31	159	71	199	1.02e-20	88.2

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Find 42 sgRNAs with CRISPR-Local

Find 248 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGAGGGCGTAAGGAAA+GGG	0.530997	5_1:+14426445	Msa0719350:CDS
AACCCAACAAATGTATCAGT+TGG	0.579773	5_1:+14428318	Msa0719350:CDS
AAGGTAGCTGTGTCTTGAGG+AGG	0.587982	5_1:-14428265	None:intergenic
AGGCCAACTGATACATTTGT+TGG	0.437602	5_1:-14428321	None:intergenic
AGTACCGATTGGATAAAGCA+AGG	0.686649	5_1:-14428341	None:intergenic
ATATGTAGGTCGTTTCTACA+GGG	0.546671	5_1:+14428022	Msa0719350:intron
ATCATATTGATCTAAATGAG+AGG	0.625540	5_1:+14426237	Msa0719350:CDS
ATGTAGGTCGTTTCTACAGG+GGG	0.657825	5_1:+14428024	Msa0719350:intron
ATGTTATCATCATTAGAGTT+TGG	0.374150	5_1:-14426353	None:intergenic
ATTAGAGTTTGGAATTGCGA+CGG	0.624770	5_1:-14426342	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTAAGTATTCAATCTATCCT+TGG	+	chr5_1:14426493-14426512	Msa0719350:intron	30.0%
AACAAACAGATACAAAGACT+GGG	-	chr5_1:14426678-14426697	None:intergenic	30.0%
AAACAAACAGATACAAAGAC+TGG	-	chr5_1:14426679-14426698	None:intergenic	30.0%
GGAATAATCTACAAAAATCG+AGG	+	chr5_1:14427011-14427030	Msa0719350:intron	30.0%
AGTAGACTTATAAAAAGGGA+CGG	+	chr5_1:14427286-14427305	Msa0719350:intron	30.0%
ATTAGCTATGTGATGACAAT+TGG	-	chr5_1:14427460-14427479	None:intergenic	30.0%
AAGAGACTATTATTGTGAGA+CGG	-	chr5_1:14427576-14427595	None:intergenic	30.0%
CCCTTATTAATTGTAGTTAG+TGG	-	chr5_1:14427713-14427732	None:intergenic	30.0%
TTGAATATCAGTAAAGTCAG+AGG	+	chr5_1:14427895-14427914	Msa0719350:intron	30.0%
GTAAGTATTCAATCTATCCT+TGG	+	chr5_1:14426493-14426512	Msa0719350:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	14426206	14428432	14426206	ID=Msa0719350;Name=Msa0719350
chr5_1	mRNA	14426206	14428432	14426206	ID=Msa0719350-mRNA-1;Parent=Msa0719350;Name=Msa0719350-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0\|0\|0\|1\|1\|1\|2\|0\|229
chr5_1	exon	14426206	14426492	14426206	ID=Msa0719350-mRNA-1:exon:5666;Parent=Msa0719350-mRNA-1
chr5_1	exon	14428030	14428432	14428030	ID=Msa0719350-mRNA-1:exon:5667;Parent=Msa0719350-mRNA-1
chr5_1	CDS	14426206	14426492	14426206	ID=Msa0719350-mRNA-1:cds;Parent=Msa0719350-mRNA-1
chr5_1	CDS	14428030	14428432	14428030	ID=Msa0719350-mRNA-1:cds;Parent=Msa0719350-mRNA-1

Gene Sequence

>Msa0719350

ATGGAAGGAAGAAAAGTTCAACAATTACCTCATCATATTGATCTAAATGAGAGGACTTCAATTCAAGATCCCCCTGAGAATAGCATGGACCAACAACAAGAGAAACTTGACACTGATGTCGTTGAGTCGGGTTGTGCCGTCGCAATTCCAAACTCTAATGATGATAACATTGCGACTCTAAACTCTAATGATAATAACTTAGTTCAAGCTGAGTCAGTACCAGAAGGAAGTCATGAAGCAAAGAGAGGGCGTAAGGAAAGGGTACATTGGACGGAAGGTGAACATAAGTCGTTTCTACAGGGGGTGAAAAAACATGGTAGGGGACGTTGGAAAGATATTTCAAAAGAATTTGTGCGAACAAAAACTCCAACTCAAATTGCTAGTCATGCTCAAAAATACTTTGTTCACCACCAAACTGCCAAAGAAATAGAGAAGAAGAAAAAGAGAAGAAGTATTCACGACATAACCTTAAACAACAATGACACAGTAGTCACTCTCCCTCTTGAAAAACAACTTGAAATTCCTCCTCAAGACACAGCTACCTTAAATAACAAGGACAATGACAACTTGCAACAAACCCAACAAATGTATCAGTTGGCCTTGCTTTATCCAATCGGTACTGCACTTCCTGATATGAACAAACTTGAGAAGATGCGCAACTTGTTTAACTTGTTGGCAAAAGACCTTTGA

Protein sequence

>Msa0719350

MEGRKVQQLPHHIDLNERTSIQDPPENSMDQQQEKLDTDVVESGCAVAIPNSNDDNIATLNSNDNNLVQAESVPEGSHEAKRGRKERVHWTEGEHKSFLQGVKKHGRGRWKDISKEFVRTKTPTQIASHAQKYFVHHQTAKEIEKKKKRRSIHDITLNNNDTVVTLPLEKQLEIPPQDTATLNNKDNDNLQQTQQMYQLALLYPIGTALPDMNKLEKMRNLFNLLAKDL*