AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0722670


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0722670 MtrunA17_Chr8g0392301 79.310 58 12 0 6 63 874 931 1.35e-25 97.4
Msa0722670 MtrunA17_Chr8g0377481 68.421 57 18 0 7 63 855 911 1.92e-20 82.8
Msa0722670 MtrunA17_Chr3g0122391 65.517 58 20 0 7 64 913 970 1.55e-19 80.1
Msa0722670 MtrunA17_Chr5g0447641 59.322 59 24 0 6 64 1087 1145 4.54e-19 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0722670 AT2G01830.5 72.414 58 16 0 6 63 947 1004 2.13e-23 91.7
Msa0722670 AT2G01830.2 72.414 58 16 0 6 63 958 1015 2.15e-23 91.7
Msa0722670 AT2G01830.1 72.414 58 16 0 6 63 935 992 2.18e-23 91.7
Msa0722670 AT2G01830.4 72.414 58 16 0 6 63 935 992 2.18e-23 91.7
Msa0722670 AT2G01830.6 72.414 58 16 0 6 63 935 992 2.18e-23 91.7
Msa0722670 AT2G01830.3 72.414 58 16 0 6 63 935 992 2.18e-23 91.7
Msa0722670 AT5G35750.1 67.241 58 19 0 6 63 1048 1105 9.03e-21 84.0
Msa0722670 AT1G27320.1 67.241 58 19 0 6 63 903 960 2.25e-20 82.8

Find 14 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCTCCATCATCTGAATT+TGG 0.221341 5_1:+20081720 None:intergenic
AAGCAATACCCATCCATTTC+TGG 0.235303 5_1:+20082538 None:intergenic
TAAAGTTGATGCTTGCTTCA+TGG 0.312853 5_1:-20082572 Msa0722670:CDS
GGACATTCAAATGCCAGAAA+TGG 0.371181 5_1:-20082551 Msa0722670:CDS
GCATCTGCAGCTACCCTCTC+AGG 0.458428 5_1:+20082670 None:intergenic
AAGCAAGCATCAACTTTATG+AGG 0.502372 5_1:+20082577 None:intergenic
AACTAACCAAATTCAGATGA+TGG 0.535150 5_1:-20081726 Msa0722670:CDS
ATTCAAATGCCAGAAATGGA+TGG 0.546140 5_1:-20082547 Msa0722670:CDS
GACAATTGCGTAAACCTGAG+AGG 0.559612 5_1:-20082684 None:intergenic
TTCAAATGCCAGAAATGGAT+GGG 0.564303 5_1:-20082546 Msa0722670:intron
ACAATTGCGTAAACCTGAGA+GGG 0.621778 5_1:-20082683 None:intergenic
AAAGTTTGGAGTTGATGTGA+AGG 0.654433 5_1:-20082641 Msa0722670:CDS
AAGTTTGGAGTTGATGTGAA+GGG 0.665780 5_1:-20082640 Msa0722670:CDS
GATGTGAAGGGTGTTGAAAG+CGG 0.718364 5_1:-20082628 Msa0722670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATATGTATAATAAAGC+AGG + chr5_1:20081884-20081903 None:intergenic 15.0%
!!! TTATAGTTATTTTGAAACTT+TGG - chr5_1:20081906-20081925 Msa0722670:intron 15.0%
!!! ATAATTTTGATGTATTTTTC+AGG - chr5_1:20082634-20082653 Msa0722670:CDS 15.0%
!! TATAATATGTATAATAAAGC+AGG + chr5_1:20081884-20081903 None:intergenic 15.0%
!!! TTATAGTTATTTTGAAACTT+TGG - chr5_1:20081906-20081925 Msa0722670:intron 15.0%
!!! ATAATTTTGATGTATTTTTC+AGG - chr5_1:20082634-20082653 Msa0722670:CDS 15.0%
!! AAATTGATTACATTACTACA+AGG + chr5_1:20082195-20082214 None:intergenic 20.0%
!! AAATTGATTACATTACTACA+AGG + chr5_1:20082195-20082214 None:intergenic 20.0%
!!! TATTTTGAAACTTTGGATGA+AGG - chr5_1:20081913-20081932 Msa0722670:intron 25.0%
! AAGGTAATGACTTTGTATAT+TGG - chr5_1:20081932-20081951 Msa0722670:intron 25.0%
! CATTACTACAAGGATTTAAT+CGG + chr5_1:20082185-20082204 None:intergenic 25.0%
!! AATCAATTTGCTTCAAATCT+TGG - chr5_1:20082206-20082225 Msa0722670:intron 25.0%
! TAGTATATGTTGAAGTATAG+GGG + chr5_1:20082401-20082420 None:intergenic 25.0%
! CTAGTATATGTTGAAGTATA+GGG + chr5_1:20082402-20082421 None:intergenic 25.0%
! TCTAGTATATGTTGAAGTAT+AGG + chr5_1:20082403-20082422 None:intergenic 25.0%
! CTACTAAAGTAATTCATTTC+AGG - chr5_1:20082555-20082574 Msa0722670:CDS 25.0%
! ACAAGTTATATGAAATGGAA+AGG + chr5_1:20082589-20082608 None:intergenic 25.0%
!! ATTTCATATAACTTGTGCAT+CGG - chr5_1:20082593-20082612 Msa0722670:CDS 25.0%
!!! TATTTTGAAACTTTGGATGA+AGG - chr5_1:20081913-20081932 Msa0722670:intron 25.0%
! AAGGTAATGACTTTGTATAT+TGG - chr5_1:20081932-20081951 Msa0722670:intron 25.0%
! CATTACTACAAGGATTTAAT+CGG + chr5_1:20082185-20082204 None:intergenic 25.0%
!! AATCAATTTGCTTCAAATCT+TGG - chr5_1:20082206-20082225 Msa0722670:intron 25.0%
! TAGTATATGTTGAAGTATAG+GGG + chr5_1:20082401-20082420 None:intergenic 25.0%
! CTAGTATATGTTGAAGTATA+GGG + chr5_1:20082402-20082421 None:intergenic 25.0%
! TCTAGTATATGTTGAAGTAT+AGG + chr5_1:20082403-20082422 None:intergenic 25.0%
! CTACTAAAGTAATTCATTTC+AGG - chr5_1:20082555-20082574 Msa0722670:CDS 25.0%
! ACAAGTTATATGAAATGGAA+AGG + chr5_1:20082589-20082608 None:intergenic 25.0%
!! ATTTCATATAACTTGTGCAT+CGG - chr5_1:20082593-20082612 Msa0722670:CDS 25.0%
GCAGATGCATTAAAAAAGTT+TGG - chr5_1:20081736-20081755 Msa0722670:CDS 30.0%
TTAATCACATCTTACCATGT+AGG - chr5_1:20082059-20082078 Msa0722670:intron 30.0%
CTAATCATATCTCACTATTC+TGG + chr5_1:20082123-20082142 None:intergenic 30.0%
TTGATAAGCACCTAAATACT+TGG - chr5_1:20082260-20082279 Msa0722670:intron 30.0%
! GTCTTTTTCTCCAAGTATTT+AGG + chr5_1:20082273-20082292 None:intergenic 30.0%
ATGATAGAGTCGTCAATTAT+GGG + chr5_1:20082371-20082390 None:intergenic 30.0%
TATGATAGAGTCGTCAATTA+TGG + chr5_1:20082372-20082391 None:intergenic 30.0%
ACCTTAAAAACTAGCTATCA+AGG - chr5_1:20082518-20082537 Msa0722670:intron 30.0%
CCTTAAAAACTAGCTATCAA+GGG - chr5_1:20082519-20082538 Msa0722670:intron 30.0%
CTTAAAAACTAGCTATCAAG+GGG - chr5_1:20082520-20082539 Msa0722670:intron 30.0%
TTAAAAACTAGCTATCAAGG+GGG - chr5_1:20082521-20082540 Msa0722670:intron 30.0%
GATGCACAAGTTATATGAAA+TGG + chr5_1:20082594-20082613 None:intergenic 30.0%
!!! TGATGTATTTTTCAGGTTTG+AGG - chr5_1:20082641-20082660 Msa0722670:CDS 30.0%
AACTAACCAAATTCAGATGA+TGG - chr5_1:20082665-20082684 Msa0722670:CDS 30.0%
GCAGATGCATTAAAAAAGTT+TGG - chr5_1:20081736-20081755 Msa0722670:CDS 30.0%
TTAATCACATCTTACCATGT+AGG - chr5_1:20082059-20082078 Msa0722670:intron 30.0%
CTAATCATATCTCACTATTC+TGG + chr5_1:20082123-20082142 None:intergenic 30.0%
TTGATAAGCACCTAAATACT+TGG - chr5_1:20082260-20082279 Msa0722670:intron 30.0%
! GTCTTTTTCTCCAAGTATTT+AGG + chr5_1:20082273-20082292 None:intergenic 30.0%
ATGATAGAGTCGTCAATTAT+GGG + chr5_1:20082371-20082390 None:intergenic 30.0%
TATGATAGAGTCGTCAATTA+TGG + chr5_1:20082372-20082391 None:intergenic 30.0%
ACCTTAAAAACTAGCTATCA+AGG - chr5_1:20082518-20082537 Msa0722670:intron 30.0%
CCTTAAAAACTAGCTATCAA+GGG - chr5_1:20082519-20082538 Msa0722670:intron 30.0%
CTTAAAAACTAGCTATCAAG+GGG - chr5_1:20082520-20082539 Msa0722670:intron 30.0%
TTAAAAACTAGCTATCAAGG+GGG - chr5_1:20082521-20082540 Msa0722670:intron 30.0%
GATGCACAAGTTATATGAAA+TGG + chr5_1:20082594-20082613 None:intergenic 30.0%
!!! TGATGTATTTTTCAGGTTTG+AGG - chr5_1:20082641-20082660 Msa0722670:CDS 30.0%
AACTAACCAAATTCAGATGA+TGG - chr5_1:20082665-20082684 Msa0722670:CDS 30.0%
!! AAAGTTTGGAGTTGATGTGA+AGG - chr5_1:20081750-20081769 Msa0722670:intron 35.0%
!! AAGTTTGGAGTTGATGTGAA+GGG - chr5_1:20081751-20081770 Msa0722670:intron 35.0%
AAGCAAGCATCAACTTTATG+AGG + chr5_1:20081817-20081836 None:intergenic 35.0%
!! TAAAGTTGATGCTTGCTTCA+TGG - chr5_1:20081819-20081838 Msa0722670:intron 35.0%
ATTCAAATGCCAGAAATGGA+TGG - chr5_1:20081844-20081863 Msa0722670:intron 35.0%
TTCAAATGCCAGAAATGGAT+GGG - chr5_1:20081845-20081864 Msa0722670:intron 35.0%
! GGTGTTTGTAGAGACATTTT+AGG - chr5_1:20082080-20082099 Msa0722670:intron 35.0%
ACTTAAAAGCTAGCAGTCAA+GGG - chr5_1:20082291-20082310 Msa0722670:intron 35.0%
TGATAGAGTCGTCAATTATG+GGG + chr5_1:20082370-20082389 None:intergenic 35.0%
TTCAAAATTGTAGCCCTTTC+TGG - chr5_1:20082434-20082453 Msa0722670:intron 35.0%
TGTGATCAGTGACTTAGAAA+GGG + chr5_1:20082486-20082505 None:intergenic 35.0%
TTGTGATCAGTGACTTAGAA+AGG + chr5_1:20082487-20082506 None:intergenic 35.0%
!!! CCCTTGATAGCTAGTTTTTA+AGG + chr5_1:20082522-20082541 None:intergenic 35.0%
TAAAAACTAGCTATCAAGGG+GGG - chr5_1:20082522-20082541 Msa0722670:intron 35.0%
TGTTCTCCATCATCTGAATT+TGG + chr5_1:20082674-20082693 None:intergenic 35.0%
!! AAAGTTTGGAGTTGATGTGA+AGG - chr5_1:20081750-20081769 Msa0722670:intron 35.0%
!! AAGTTTGGAGTTGATGTGAA+GGG - chr5_1:20081751-20081770 Msa0722670:intron 35.0%
AAGCAAGCATCAACTTTATG+AGG + chr5_1:20081817-20081836 None:intergenic 35.0%
!! TAAAGTTGATGCTTGCTTCA+TGG - chr5_1:20081819-20081838 Msa0722670:intron 35.0%
ATTCAAATGCCAGAAATGGA+TGG - chr5_1:20081844-20081863 Msa0722670:intron 35.0%
TTCAAATGCCAGAAATGGAT+GGG - chr5_1:20081845-20081864 Msa0722670:intron 35.0%
! GGTGTTTGTAGAGACATTTT+AGG - chr5_1:20082080-20082099 Msa0722670:intron 35.0%
ACTTAAAAGCTAGCAGTCAA+GGG - chr5_1:20082291-20082310 Msa0722670:intron 35.0%
TGATAGAGTCGTCAATTATG+GGG + chr5_1:20082370-20082389 None:intergenic 35.0%
TTCAAAATTGTAGCCCTTTC+TGG - chr5_1:20082434-20082453 Msa0722670:intron 35.0%
TGTGATCAGTGACTTAGAAA+GGG + chr5_1:20082486-20082505 None:intergenic 35.0%
TTGTGATCAGTGACTTAGAA+AGG + chr5_1:20082487-20082506 None:intergenic 35.0%
!!! CCCTTGATAGCTAGTTTTTA+AGG + chr5_1:20082522-20082541 None:intergenic 35.0%
TAAAAACTAGCTATCAAGGG+GGG - chr5_1:20082522-20082541 Msa0722670:intron 35.0%
TGTTCTCCATCATCTGAATT+TGG + chr5_1:20082674-20082693 None:intergenic 35.0%
GGACATTCAAATGCCAGAAA+TGG - chr5_1:20081840-20081859 Msa0722670:intron 40.0%
AAGCAATACCCATCCATTTC+TGG + chr5_1:20081856-20081875 None:intergenic 40.0%
TGTCTCTACAAACACCTACA+TGG + chr5_1:20082076-20082095 None:intergenic 40.0%
GACTTAAAAGCTAGCAGTCA+AGG - chr5_1:20082290-20082309 Msa0722670:intron 40.0%
CTTAAAAGCTAGCAGTCAAG+GGG - chr5_1:20082292-20082311 Msa0722670:intron 40.0%
TTATGGGGTTCCTAAGTCAT+GGG + chr5_1:20082355-20082374 None:intergenic 40.0%
ATTATGGGGTTCCTAAGTCA+TGG + chr5_1:20082356-20082375 None:intergenic 40.0%
TAGAAAGGGCTACCAACTAA+GGG + chr5_1:20082472-20082491 None:intergenic 40.0%
TTAGAAAGGGCTACCAACTA+AGG + chr5_1:20082473-20082492 None:intergenic 40.0%
AAAAACTAGCTATCAAGGGG+GGG - chr5_1:20082523-20082542 Msa0722670:intron 40.0%
!! TATAACTTGTGCATCGGCTT+TGG - chr5_1:20082599-20082618 Msa0722670:CDS 40.0%
!! ATAACTTGTGCATCGGCTTT+GGG - chr5_1:20082600-20082619 Msa0722670:CDS 40.0%
GGACATTCAAATGCCAGAAA+TGG - chr5_1:20081840-20081859 Msa0722670:intron 40.0%
AAGCAATACCCATCCATTTC+TGG + chr5_1:20081856-20081875 None:intergenic 40.0%
TGTCTCTACAAACACCTACA+TGG + chr5_1:20082076-20082095 None:intergenic 40.0%
GACTTAAAAGCTAGCAGTCA+AGG - chr5_1:20082290-20082309 Msa0722670:intron 40.0%
CTTAAAAGCTAGCAGTCAAG+GGG - chr5_1:20082292-20082311 Msa0722670:intron 40.0%
TTATGGGGTTCCTAAGTCAT+GGG + chr5_1:20082355-20082374 None:intergenic 40.0%
ATTATGGGGTTCCTAAGTCA+TGG + chr5_1:20082356-20082375 None:intergenic 40.0%
TAGAAAGGGCTACCAACTAA+GGG + chr5_1:20082472-20082491 None:intergenic 40.0%
TTAGAAAGGGCTACCAACTA+AGG + chr5_1:20082473-20082492 None:intergenic 40.0%
AAAAACTAGCTATCAAGGGG+GGG - chr5_1:20082523-20082542 Msa0722670:intron 40.0%
!! TATAACTTGTGCATCGGCTT+TGG - chr5_1:20082599-20082618 Msa0722670:CDS 40.0%
!! ATAACTTGTGCATCGGCTTT+GGG - chr5_1:20082600-20082619 Msa0722670:CDS 40.0%
!! GATGTGAAGGGTGTTGAAAG+CGG - chr5_1:20081763-20081782 Msa0722670:intron 45.0%
!! TCGGAGTACTTAAGTTGGCT+TGG + chr5_1:20082324-20082343 None:intergenic 45.0%
TTCCTAAGTCATGGGATGCT+CGG + chr5_1:20082347-20082366 None:intergenic 45.0%
AGAAAGGGCTACCAACTAAG+GGG + chr5_1:20082471-20082490 None:intergenic 45.0%
!! GATGTGAAGGGTGTTGAAAG+CGG - chr5_1:20081763-20081782 Msa0722670:intron 45.0%
!! TCGGAGTACTTAAGTTGGCT+TGG + chr5_1:20082324-20082343 None:intergenic 45.0%
TTCCTAAGTCATGGGATGCT+CGG + chr5_1:20082347-20082366 None:intergenic 45.0%
AGAAAGGGCTACCAACTAAG+GGG + chr5_1:20082471-20082490 None:intergenic 45.0%
!!! TTTTATATTAATAGTTTAAT+TGG - chr5_1:20082001-20082020 Msa0722670:intron 5.0%
!!! TTTTATATTAATAGTTTAAT+TGG - chr5_1:20082001-20082020 Msa0722670:intron 5.0%
! CTCGGTCGGAGTACTTAAGT+TGG + chr5_1:20082329-20082348 None:intergenic 50.0%
TAAGTCATGGGATGCTCGGT+CGG + chr5_1:20082343-20082362 None:intergenic 50.0%
! TTGTAGCCCTTTCTGGTCAG+TGG - chr5_1:20082441-20082460 Msa0722670:intron 50.0%
GAAAGGGCTACCAACTAAGG+GGG + chr5_1:20082470-20082489 None:intergenic 50.0%
! CTCGGTCGGAGTACTTAAGT+TGG + chr5_1:20082329-20082348 None:intergenic 50.0%
TAAGTCATGGGATGCTCGGT+CGG + chr5_1:20082343-20082362 None:intergenic 50.0%
! TTGTAGCCCTTTCTGGTCAG+TGG - chr5_1:20082441-20082460 Msa0722670:intron 50.0%
GAAAGGGCTACCAACTAAGG+GGG + chr5_1:20082470-20082489 None:intergenic 50.0%
GACCGAGCATCCCATGACTT+AGG - chr5_1:20082342-20082361 Msa0722670:intron 55.0%
GGGTGACCACTGACCAGAAA+GGG + chr5_1:20082450-20082469 None:intergenic 55.0%
TCAGTGGTCACCCCCTTAGT+TGG - chr5_1:20082457-20082476 Msa0722670:intron 55.0%
GACCGAGCATCCCATGACTT+AGG - chr5_1:20082342-20082361 Msa0722670:intron 55.0%
GGGTGACCACTGACCAGAAA+GGG + chr5_1:20082450-20082469 None:intergenic 55.0%
TCAGTGGTCACCCCCTTAGT+TGG - chr5_1:20082457-20082476 Msa0722670:intron 55.0%
GCATCTGCAGCTACCCTCTC+AGG + chr5_1:20081724-20081743 None:intergenic 60.0%
GGGGTGACCACTGACCAGAA+AGG + chr5_1:20082451-20082470 None:intergenic 60.0%
GCATCTGCAGCTACCCTCTC+AGG + chr5_1:20081724-20081743 None:intergenic 60.0%
GGGGTGACCACTGACCAGAA+AGG + chr5_1:20082451-20082470 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 20081712 20082701 20081712 ID=Msa0722670;Name=Msa0722670
chr5_1 mRNA 20081712 20082701 20081712 ID=Msa0722670-mRNA-1;Parent=Msa0722670;Name=Msa0722670-mRNA-1;_AED=0.59;_eAED=0.63;_QI=0|0|0|0.5|1|1|2|0|66
chr5_1 exon 20082547 20082701 20082547 ID=Msa0722670-mRNA-1:exon:7602;Parent=Msa0722670-mRNA-1
chr5_1 exon 20081712 20081757 20081712 ID=Msa0722670-mRNA-1:exon:7601;Parent=Msa0722670-mRNA-1
chr5_1 CDS 20082547 20082701 20082547 ID=Msa0722670-mRNA-1:cds;Parent=Msa0722670-mRNA-1
chr5_1 CDS 20081712 20081757 20081712 ID=Msa0722670-mRNA-1:cds;Parent=Msa0722670-mRNA-1
Gene Sequence

>Msa0722670

TTGCGTAAACCTGAGAGGGTAGCTGCAGATGCATTAAAAAAGTTTGGAGTTGATGTGAAGGGTGTTGAAAGCGGCAAAGCTGCACTTGAAATGCTTCAATTGCCTCATAAAGTTGATGCTTGCTTCATGGACATTCAAATGCCAGAAATGGATGGGTTTGAGGCAACTAACCAAATTCAGATGATGGAGAACAGAAAATGA

Protein sequence

>Msa0722670

LRKPERVAADALKKFGVDVKGVESGKAALEMLQLPHKVDACFMDIQMPEMDGFEATNQIQMMENRK*