AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0723190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0723190 MtrunA17_Chr5g0414041 91.419 303 5 3 4 285 142 444 0.0 566
Msa0723190 MtrunA17_Chr5g0414061 70.234 299 66 5 4 281 149 445 7.44e-152 441
Msa0723190 MtrunA17_Chr5g0414091 66.216 296 79 3 4 278 140 435 1.28e-141 414
Msa0723190 MtrunA17_Chr5g0414031 87.764 237 9 1 293 509 94 330 2.86e-133 389
Msa0723190 MtrunA17_Chr3g0140371 58.086 303 101 5 1 281 69 367 4.19e-121 360
Msa0723190 MtrunA17_Chr5g0414051 58.766 308 97 7 4 285 130 433 2.69e-120 360
Msa0723190 MtrunA17_Chr8g0391481 54.237 295 109 6 4 275 82 373 3.71e-112 337
Msa0723190 MtrunA17_Chr7g0226991 53.378 296 112 6 4 276 112 404 5.55e-108 327
Msa0723190 MtrunA17_Chr5g0414081 64.677 201 58 5 88 276 2 201 1.83e-89 273
Msa0723190 MtrunA17_Chr8g0347321 41.924 291 148 6 4 274 150 439 3.79e-70 231
Msa0723190 MtrunA17_Chr6g0456691 41.077 297 141 10 4 275 133 420 1.03e-69 229
Msa0723190 MtrunA17_Chr8g0387921 39.726 292 154 6 4 274 129 419 5.20e-64 214
Msa0723190 MtrunA17_Chr6g0451321 36.156 307 167 8 4 288 172 471 1.37e-57 198
Msa0723190 MtrunA17_Chr2g0283851 35.526 304 155 11 4 276 109 402 1.05e-53 186
Msa0723190 MtrunA17_Chr6g0456681 37.874 301 148 10 4 275 119 409 1.01e-51 181
Msa0723190 MtrunA17_Chr7g0221281 38.247 251 118 8 37 275 12 237 6.47e-50 172
Msa0723190 MtrunA17_Chr5g0413991 87.356 87 11 0 423 509 20 106 1.50e-47 160
Msa0723190 MtrunA17_Chr5g0414011 87.143 70 9 0 440 509 1 70 3.99e-37 131
Msa0723190 MtrunA17_Chr1g0188191 30.323 310 164 11 4 276 289 583 7.19e-37 144
Msa0723190 MtrunA17_Chr6g0476041 31.310 313 171 12 4 283 222 523 1.61e-36 142
Msa0723190 MtrunA17_Chr5g0414001 52.991 117 30 3 295 391 171 282 1.89e-28 114
Msa0723190 MtrunA17_Chr7g0259711 30.503 318 176 16 4 289 146 450 5.50e-28 116
Msa0723190 MtrunA17_Chr1g0175671 31.104 299 157 13 10 276 231 512 2.59e-24 106
Msa0723190 MtrunA17_Chr7g0258851 31.023 303 159 14 10 279 309 594 6.36e-24 105
Msa0723190 MtrunA17_Chr2g0287901 30.363 303 170 14 4 278 152 441 1.08e-22 100
Msa0723190 MtrunA17_Chr1g0213281 29.841 315 158 16 10 283 236 528 2.67e-22 100
Msa0723190 MtrunA17_Chr7g0269991 30.952 294 156 17 4 271 192 464 6.82e-22 98.6
Msa0723190 MtrunA17_Chr4g0068111 26.821 302 172 12 4 274 170 453 3.05e-21 96.7
Msa0723190 MtrunA17_Chr4g0070341 27.419 310 160 12 1 271 185 468 3.48e-21 96.3
Msa0723190 MtrunA17_Chr6g0455901 25.163 306 179 11 1 272 125 414 4.47e-20 92.4
Msa0723190 MtrunA17_Chr4g0071391 28.155 309 176 14 4 283 164 455 7.31e-20 92.4
Msa0723190 MtrunA17_Chr8g0368461 26.948 308 169 14 9 275 238 530 4.58e-19 90.1
Msa0723190 MtrunA17_Chr8g0384151 26.689 296 164 13 4 272 145 414 6.75e-19 89.0
Msa0723190 MtrunA17_Chr5g0413271 26.280 293 172 12 9 276 205 478 4.66e-18 87.0
Msa0723190 MtrunA17_Chr7g0275501 54.098 61 25 1 213 273 11 68 1.23e-17 77.4
Msa0723190 MtrunA17_Chr1g0178081 28.364 275 153 10 4 276 112 344 1.33e-17 84.3
Msa0723190 MtrunA17_Chr4g0006991 27.365 296 180 13 4 274 142 427 2.26e-17 84.3
Msa0723190 MtrunA17_Chr5g0430371 25.589 297 177 12 4 270 113 395 2.82e-17 84.0
Msa0723190 MtrunA17_Chr7g0242701 29.293 297 173 16 4 274 142 427 1.56e-16 81.6
Msa0723190 MtrunA17_Chr2g0314801 25.939 293 167 12 4 274 89 353 3.33e-16 80.1
Msa0723190 MtrunA17_Chr8g0384421 23.701 308 174 10 1 271 186 469 3.70e-16 80.9
Msa0723190 MtrunA17_Chr4g0057171 43.158 95 44 3 79 169 1 89 4.47e-16 74.7
Msa0723190 MtrunA17_Chr3g0085081 27.208 283 166 12 4 270 86 344 4.51e-16 79.7
Msa0723190 MtrunA17_Chr3g0091031 26.056 284 168 10 4 270 86 344 6.40e-16 79.3
Msa0723190 MtrunA17_Chr3g0085071 27.273 286 162 14 4 270 101 359 8.58e-16 79.0
Msa0723190 MtrunA17_Chr4g0005491 26.689 296 178 13 4 270 118 403 1.05e-15 79.3
Msa0723190 MtrunA17_Chr7g0243301 25.086 291 189 11 4 270 120 405 1.47e-15 78.6
Msa0723190 MtrunA17_Chr3g0113671 27.667 300 168 17 4 270 162 445 1.91e-15 78.6
Msa0723190 MtrunA17_Chr3g0105301 26.207 290 176 13 4 270 98 372 5.37e-15 76.6
Msa0723190 MtrunA17_Chr1g0171171 26.644 289 174 10 4 274 107 375 1.33e-14 75.5
Msa0723190 MtrunA17_Chr2g0325481 28.214 280 164 13 4 269 105 361 1.93e-14 75.1
Msa0723190 MtrunA17_Chr3g0126021 26.599 297 167 15 4 274 188 459 2.32e-14 75.5
Msa0723190 MtrunA17_Chr2g0314791 27.698 278 164 12 4 270 90 341 5.63e-14 73.2
Msa0723190 MtrunA17_Chr3g0097891 24.643 280 179 7 4 270 86 346 2.40e-13 71.2
Msa0723190 MtrunA17_Chr2g0283581 26.071 280 181 9 4 274 96 358 2.67e-13 71.2
Msa0723190 MtrunA17_Chr8g0360521 24.756 307 175 12 4 276 127 411 2.86e-13 71.6
Msa0723190 MtrunA17_Chr5g0413851 26.950 282 170 12 4 270 95 355 8.61e-13 69.7
Msa0723190 MtrunA17_Chr4g0041011 25.087 287 180 11 1 274 99 363 2.28e-12 68.6
Msa0723190 MtrunA17_Chr5g0437291 25.828 302 180 15 1 270 218 507 3.01e-12 68.9
Msa0723190 MtrunA17_Chr4g0005481 28.188 298 154 16 13 274 173 446 3.67e-12 68.6
Msa0723190 MtrunA17_Chr4g0045951 24.911 281 169 11 4 267 100 355 3.75e-12 67.8
Msa0723190 MtrunA17_Chr7g0221271 45.312 64 34 1 4 67 134 196 1.93e-11 63.5
Msa0723190 MtrunA17_Chr5g0437601 26.351 296 177 13 4 271 139 421 2.49e-11 65.9
Msa0723190 MtrunA17_Chr4g0045941 24.014 279 174 12 4 267 88 343 5.50e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0723190 AT1G70230.1 57.986 288 102 6 4 278 134 415 5.50e-117 351
Msa0723190 AT1G24040.3 53.086 243 89 5 278 497 62 302 1.68e-77 246
Msa0723190 AT1G24040.2 53.086 243 89 5 278 497 62 302 1.68e-77 246
Msa0723190 AT1G24040.1 53.086 243 89 5 278 497 62 302 1.68e-77 246
Msa0723190 AT4G01080.1 42.456 285 146 9 4 272 146 428 9.48e-69 227
Msa0723190 AT1G01430.1 40.339 295 151 8 4 274 154 447 1.69e-67 224
Msa0723190 AT5G15890.1 39.249 293 146 9 4 275 242 523 5.20e-64 217
Msa0723190 AT3G02440.2 38.796 299 145 9 4 275 190 477 9.39e-63 213
Msa0723190 AT3G02440.1 38.796 299 145 9 4 275 182 469 1.08e-62 212
Msa0723190 AT5G15900.1 39.000 300 150 10 4 279 124 414 1.44e-61 208
Msa0723190 AT5G15900.2 39.000 300 150 10 4 279 143 433 2.64e-61 207
Msa0723190 AT4G23790.1 37.755 294 159 7 4 274 135 427 5.87e-59 201
Msa0723190 AT4G11090.1 38.356 292 158 7 4 274 135 425 5.16e-57 196
Msa0723190 AT3G28150.1 35.811 296 159 9 4 276 125 412 1.51e-54 189
Msa0723190 AT4G25360.1 32.166 314 155 13 4 276 230 526 1.55e-38 148
Msa0723190 AT4G25360.2 32.166 314 155 13 4 276 230 526 1.55e-38 148
Msa0723190 AT5G51640.1 31.046 306 170 11 4 276 193 490 1.08e-36 142
Msa0723190 AT3G06080.2 31.475 305 166 15 4 278 158 449 8.91e-27 113
Msa0723190 AT3G11570.1 31.633 294 159 15 4 270 137 415 9.15e-26 110
Msa0723190 AT5G64020.1 27.832 309 172 11 1 271 110 405 3.02e-25 108
Msa0723190 AT5G19160.1 29.073 313 180 12 4 284 154 456 5.47e-25 108
Msa0723190 AT2G14530.1 25.974 308 171 11 4 274 119 406 5.79e-25 107
Msa0723190 AT5G20590.1 30.743 296 159 14 4 270 197 475 5.25e-23 102
Msa0723190 AT3G12060.1 29.492 295 156 15 9 270 256 531 2.46e-22 100
Msa0723190 AT1G48880.1 29.966 297 149 15 4 271 170 436 4.87e-20 93.2
Msa0723190 AT5G64470.3 26.490 302 172 11 4 274 112 394 9.70e-20 91.7
Msa0723190 AT5G20680.4 25.083 303 182 9 1 274 265 551 1.48e-19 92.4
Msa0723190 AT5G20680.1 25.083 303 182 9 1 274 265 551 1.48e-19 92.4
Msa0723190 AT5G20680.3 25.083 303 182 9 1 274 265 551 1.48e-19 92.4
Msa0723190 AT5G20680.5 25.083 303 182 9 1 274 265 551 1.48e-19 92.4
Msa0723190 AT5G20680.2 25.083 303 182 9 1 274 247 533 1.98e-19 91.7
Msa0723190 AT5G20680.6 26.073 303 179 11 1 274 171 457 2.42e-19 91.3
Msa0723190 AT5G49340.1 27.365 296 181 12 4 274 155 441 3.09e-19 90.9
Msa0723190 AT5G64470.2 26.299 308 171 12 4 274 112 400 5.73e-19 89.4
Msa0723190 AT5G06230.2 26.621 293 175 11 4 270 82 360 6.91e-19 89.0
Msa0723190 AT5G06230.1 26.621 293 175 11 4 270 123 401 9.06e-19 89.0
Msa0723190 AT3G62390.1 28.155 309 159 16 4 276 193 474 2.48e-18 88.2
Msa0723190 AT1G60790.1 26.645 304 165 13 9 275 252 534 2.48e-18 88.6
Msa0723190 AT2G37720.1 24.503 302 173 10 4 271 199 479 8.67e-18 86.7
Msa0723190 AT1G73140.1 27.645 293 172 12 4 270 123 401 2.75e-16 81.3
Msa0723190 AT1G78710.1 28.425 292 161 13 4 274 92 356 4.68e-16 80.1
Msa0723190 AT3G14850.2 26.990 289 170 10 4 274 89 354 4.95e-16 80.1
Msa0723190 AT2G34070.1 25.263 285 178 10 4 274 120 383 1.48e-15 79.0
Msa0723190 AT5G01360.2 26.599 297 176 13 4 271 92 375 1.67e-15 78.6
Msa0723190 AT5G01360.1 26.599 297 176 13 4 271 148 431 1.78e-15 79.0
Msa0723190 AT3G14850.1 27.106 273 158 10 20 274 2 251 3.53e-15 75.9
Msa0723190 AT2G30900.1 25.275 273 174 8 11 270 106 361 4.26e-15 77.4
Msa0723190 AT5G58600.1 25.342 292 184 11 4 274 122 400 5.91e-15 77.4
Msa0723190 AT2G42570.1 26.316 285 164 12 9 274 107 364 8.75e-15 76.3
Msa0723190 AT2G38320.2 24.915 293 187 11 4 270 32 317 1.63e-14 75.1
Msa0723190 AT2G34070.2 26.038 265 161 11 20 270 2 245 3.46e-14 72.8
Msa0723190 AT3G55990.1 27.946 297 171 14 4 270 196 479 3.88e-14 75.1
Msa0723190 AT2G38320.1 24.915 293 187 11 4 270 115 400 3.93e-14 74.7
Msa0723190 AT1G29050.1 25.874 286 174 13 4 274 116 378 5.30e-14 73.9
Msa0723190 AT2G40160.1 23.432 303 180 13 4 270 133 419 1.14e-13 73.6
Msa0723190 AT2G40160.2 23.432 303 180 13 4 270 133 419 1.26e-13 73.2
Msa0723190 AT2G31110.2 24.912 285 170 13 4 270 100 358 2.03e-13 72.0
Msa0723190 AT3G54260.1 24.913 289 180 11 4 274 105 374 2.00e-12 69.3
Msa0723190 AT5G01620.2 24.221 289 177 13 13 274 176 449 2.37e-12 69.3
Msa0723190 AT5G01620.1 24.221 289 177 13 13 274 176 449 2.37e-12 69.3
Msa0723190 AT5G01620.3 24.221 289 177 13 13 274 184 457 2.75e-12 69.3
Msa0723190 AT5G19160.2 29.101 189 103 7 4 168 154 335 8.10e-12 67.0
Msa0723190 AT3G06080.1 30.688 189 100 9 4 168 158 339 2.27e-11 65.9

Find 151 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTCTTAATCATACTGAAAT+TGG 0.102828 5_1:-20800498 Msa0723190:CDS
GCCTGCCACTTTCAAATTAA+AGG 0.112147 5_1:+20793661 None:intergenic
TCCTATCAATTATGCTATTA+AGG 0.141791 5_1:+20800439 None:intergenic
AAATCATAATAAGTTGTATT+TGG 0.178202 5_1:-20800652 Msa0723190:CDS
ACTCTTCACTCTTGAGTTCT+TGG 0.205568 5_1:+20800006 None:intergenic
CTTCTATAAGGCTTAGTCTT+TGG 0.224979 5_1:+20800327 None:intergenic
GGCACTTGATGTAACTAAAT+TGG 0.237596 5_1:-20800196 Msa0723190:CDS
ATTGGTTTGGTGTATATATA+TGG 0.247486 5_1:-20793358 Msa0723190:three_prime_UTR
AAGTTTAGACATTAGAATTA+TGG 0.252110 5_1:+20793232 None:intergenic
GAATTATGGACAAATCTATT+TGG 0.256353 5_1:+20793246 None:intergenic
AAAGAGACCTGTTGTGTTAC+AGG 0.269323 5_1:-20793921 Msa0723190:intron
GGAGAATGGAGATTGGTTTC+AGG 0.273984 5_1:-20793973 Msa0723190:three_prime_UTR
ATACTTGTGCTGATGTTAAT+TGG 0.284151 5_1:-20793376 Msa0723190:three_prime_UTR
TCCTTCTCCCTACTTCTATA+AGG 0.286857 5_1:+20800315 None:intergenic
AAATACAACTTATTATGATT+TGG 0.294595 5_1:+20800654 None:intergenic
TATAGAGGCAGTGGTGTTAA+TGG 0.295778 5_1:-20797296 Msa0723190:CDS
AGGAAATAAAGGTAGTAAAA+TGG 0.324311 5_1:-20800415 Msa0723190:CDS
TTCTGGAGATTATGAAGAAA+TGG 0.334403 5_1:-20800038 Msa0723190:CDS
CAAATATAAGGCTTATCTTT+CGG 0.336668 5_1:+20797179 None:intergenic
CCCTCAAATCCGGCACCGTT+TGG 0.340126 5_1:-20797493 Msa0723190:CDS
TGAAAGATGGGCAAAGGATA+TGG 0.342119 5_1:-20800619 Msa0723190:CDS
ACAGCCATGTTACAAATATA+AGG 0.346669 5_1:+20797167 None:intergenic
GTGGTGGAGGGTGGTGAGTT+CGG 0.353044 5_1:+20797541 None:intergenic
GGCTTATCTTTCGGCGGATC+CGG 0.353217 5_1:+20797188 None:intergenic
ATGAGGAACTAAGTCCATTA+TGG 0.354775 5_1:+20793446 None:intergenic
GCTGTCAGTCTCTTTCTCTT+AGG 0.361531 5_1:-20793560 Msa0723190:three_prime_UTR
AATGGAAAAGGGTACATATA+AGG 0.371725 5_1:+20800144 None:intergenic
TGTGTTCATTGGTGTTTGCC+TGG 0.372187 5_1:-20800087 Msa0723190:CDS
ATGAATTGAAGAAAAGTGTT+AGG 0.375554 5_1:+20800894 None:intergenic
AGACAACATTTATGTGTTCA+AGG 0.375671 5_1:-20794162 Msa0723190:three_prime_UTR
AAGGTGAAAAGTAGTGAATT+TGG 0.378681 5_1:-20800234 Msa0723190:CDS
TTGTGCTTTCAATTGGTCAT+TGG 0.379558 5_1:-20800581 Msa0723190:CDS
ATTCATTGAAAACAAACATA+TGG 0.383919 5_1:-20800877 Msa0723190:CDS
TGTGCTGATGTTAATTGGTT+TGG 0.388795 5_1:-20793371 Msa0723190:three_prime_UTR
GGTAAGTGGAGAGGTGGTGG+AGG 0.390544 5_1:+20797528 None:intergenic
ATTGATAGGAGAGGAAATAA+AGG 0.397784 5_1:-20800426 Msa0723190:CDS
CCTCAAATCCGGCACCGTTT+GGG 0.400069 5_1:-20797492 Msa0723190:CDS
CCCAAACGGTGCCGGATTTG+AGG 0.402104 5_1:+20797492 None:intergenic
GTCTTAATCATACTGAAATT+GGG 0.403211 5_1:-20800497 Msa0723190:CDS
CCTATCAATTATGCTATTAA+GGG 0.403903 5_1:+20800440 None:intergenic
TCTGGAGATTATGAAGAAAT+GGG 0.408121 5_1:-20800037 Msa0723190:CDS
AGAGGTAAGTGGAGAGGTGG+TGG 0.416599 5_1:+20797525 None:intergenic
ACCTTTAATTTGAAAGTGGC+AGG 0.420741 5_1:-20793662 Msa0723190:three_prime_UTR
GTCTACTTCTACCACACCTT+CGG 0.425828 5_1:+20793523 None:intergenic
TGCAAAATGATTGTGTTCAT+TGG 0.427697 5_1:-20800098 Msa0723190:CDS
AGACTAAGCCTTATAGAAGT+AGG 0.435118 5_1:-20800323 Msa0723190:CDS
AATACACATAAAATTTGCTC+TGG 0.436805 5_1:-20793744 Msa0723190:three_prime_UTR
ATTTGGAGGGGTTAGGTTTG+AGG 0.439421 5_1:-20800217 Msa0723190:CDS
TATATATTTCTCTTATCTGT+AGG 0.440142 5_1:-20793944 Msa0723190:three_prime_UTR
GGCAAAGGATATGGAAAAGA+TGG 0.440553 5_1:-20800610 Msa0723190:CDS
ACCCCATTAGCAAATGGAAA+AGG 0.442392 5_1:+20800132 None:intergenic
TCTCCCTTCAGGAGGGGCAT+TGG 0.444220 5_1:-20794439 Msa0723190:intron
TTAGGTTGTCATTATTGTGT+TGG 0.447247 5_1:-20800519 Msa0723190:CDS
CGGTGCCGGATTTGAGGGTG+TGG 0.449362 5_1:+20797498 None:intergenic
GAAGAGGAAATGCAGTTGGC+TGG 0.454362 5_1:-20797263 Msa0723190:CDS
TGGATCTTGTTGATGAAAGA+TGG 0.457453 5_1:-20800632 Msa0723190:CDS
CCAAACGGTGCCGGATTTGA+GGG 0.458538 5_1:+20797493 None:intergenic
TAATTCTACTCTTCCGTCGC+CGG 0.460000 5_1:-20797207 Msa0723190:CDS
TCATTTACCTGTAACACAAC+AGG 0.460609 5_1:+20793914 None:intergenic
TAAGGACCTGGATGACCATC+TGG 0.461971 5_1:+20800162 None:intergenic
GAGGAGTTTGATGCCGCATA+TGG 0.462290 5_1:-20797336 Msa0723190:CDS
GTTTACTGGAGAATGGAGAT+TGG 0.464299 5_1:-20793980 Msa0723190:three_prime_UTR
CAAGCGAGGAACTGATTTCT+CGG 0.465423 5_1:-20794399 Msa0723190:CDS
TCTTTCAGCCAGGATTGGTA+TGG 0.468462 5_1:+20793621 None:intergenic
TGCGTCTCTGCAACAACAGC+AGG 0.469429 5_1:+20794266 None:intergenic
ATCTCATTCCATGTGTCTAT+AGG 0.470243 5_1:+20800063 None:intergenic
ATTTCTCGGATGAGTTCATC+AGG 0.474442 5_1:-20794385 Msa0723190:CDS
CTGTTGATAAATCTCTTAGA+AGG 0.476108 5_1:-20797148 Msa0723190:intron
AGTGGAGAGGTGGTGGAGGG+TGG 0.476304 5_1:+20797532 None:intergenic
TTGAGACCAGATGGTCATCC+AGG 0.479603 5_1:-20800168 Msa0723190:CDS
TTCAACCTTATCATCACCCT+TGG 0.487256 5_1:+20793501 None:intergenic
GTAGACCAAGGGTGATGATA+AGG 0.487843 5_1:-20793506 Msa0723190:three_prime_UTR
TGGAGCTTTCCATCGGTCAA+TGG 0.488831 5_1:+20793266 None:intergenic
TGAAAGAAAATAATAATGCT+AGG 0.490110 5_1:-20793605 Msa0723190:three_prime_UTR
CCACTAGTGTAAACAAGATC+AGG 0.492606 5_1:+20800783 None:intergenic
GGAGAAGGAACTTGAAGGAA+TGG 0.495842 5_1:-20800301 Msa0723190:CDS
CATGGCACGTTTGTTTCTGA+AGG 0.497482 5_1:+20794205 None:intergenic
ATAAGGTTGAAGAATAGAAA+AGG 0.497657 5_1:-20793489 Msa0723190:three_prime_UTR
ATCAACCTTTAATTTGAAAG+TGG 0.498105 5_1:-20793666 Msa0723190:three_prime_UTR
TAATTTCTGTATGTGCATCA+AGG 0.498958 5_1:-20793697 Msa0723190:three_prime_UTR
TTCCATGTGTCTATAGGTCC+AGG 0.501440 5_1:+20800069 None:intergenic
CTATTGAACCATACCAATCC+TGG 0.502209 5_1:-20793629 Msa0723190:three_prime_UTR
TTGTTGGTGATTCTATGGCT+AGG 0.502299 5_1:-20800851 Msa0723190:CDS
CCTTCAGGAGGGGCATTGGA+TGG 0.505384 5_1:-20794435 Msa0723190:intron
CCCTTAATAGCATAATTGAT+AGG 0.509822 5_1:-20800440 Msa0723190:CDS
ATCTATTTGGAGCTTTCCAT+CGG 0.510726 5_1:+20793259 None:intergenic
AGTAGGGAGAAGGAACTTGA+AGG 0.514231 5_1:-20800306 Msa0723190:CDS
GACTAAGCCTTATAGAAGTA+GGG 0.514785 5_1:-20800322 Msa0723190:CDS
TACTCGAACCCAAACGGTGC+CGG 0.515116 5_1:+20797484 None:intergenic
TACACCCACACCCTCAAATC+CGG 0.516142 5_1:-20797503 Msa0723190:CDS
AGGATATTAAGTTTAGAAAG+TGG 0.516473 5_1:-20800758 Msa0723190:CDS
CATTGGATGGCATCTTCTAA+AGG 0.517341 5_1:-20794422 Msa0723190:CDS
GTAAGTGGAGAGGTGGTGGA+GGG 0.520989 5_1:+20797529 None:intergenic
CCCCATTAGCAAATGGAAAA+GGG 0.522058 5_1:+20800133 None:intergenic
GTAGTGAATTTGGAGGGGTT+AGG 0.522463 5_1:-20800224 Msa0723190:CDS
GAAAGAAAATAATAATGCTA+GGG 0.523301 5_1:-20793604 Msa0723190:three_prime_UTR
GGTGTGGGTGTAGAGGTAAG+TGG 0.523878 5_1:+20797514 None:intergenic
TGAAACAGTATTTGATTGAA+CGG 0.524045 5_1:-20797358 Msa0723190:CDS
TTTGAAAGTGGCAGGCACAT+TGG 0.524645 5_1:-20793654 Msa0723190:three_prime_UTR
CCTGATCTTGTTTACACTAG+TGG 0.531783 5_1:-20800783 Msa0723190:CDS
GGATCTTGTTGATGAAAGAT+GGG 0.537727 5_1:-20800631 Msa0723190:CDS
AAGAGGAAATGCAGTTGGCT+GGG 0.542657 5_1:-20797262 Msa0723190:CDS
AATGGTAGCAAATATGATCA+AGG 0.543075 5_1:-20794012 Msa0723190:three_prime_UTR
TGAAAAGTAGTGAATTTGGA+GGG 0.552481 5_1:-20800230 Msa0723190:CDS
TTTGATGATCTTACAAGATG+AGG 0.555846 5_1:+20793429 None:intergenic
TCTTTCGGCGGATCCGGCGA+CGG 0.556296 5_1:+20797194 None:intergenic
GGCAGTGGTGTTAATGGTGA+TGG 0.556640 5_1:-20797290 Msa0723190:CDS
TCTGGAACCCCATTAGCAAA+TGG 0.557935 5_1:+20800126 None:intergenic
TCACCTTCGTAGTAAACTGC+AGG 0.557945 5_1:+20800549 None:intergenic
CTTTCACGTGGCCGAAGGTG+TGG 0.561849 5_1:-20793534 Msa0723190:three_prime_UTR
GTGTTGAGAAATCAAATGAC+GGG 0.563717 5_1:-20800677 Msa0723190:CDS
CATTCACCTTCAAAATGAGC+TGG 0.568324 5_1:+20800369 None:intergenic
TTACAAATATTAAAGATGCA+TGG 0.570868 5_1:-20793769 Msa0723190:three_prime_UTR
ACGCATTACTCGAACCCAAA+CGG 0.571049 5_1:+20797478 None:intergenic
CACACCTTCGGCCACGTGAA+AGG 0.572439 5_1:+20793535 None:intergenic
TGTAGAGGTAAGTGGAGAGG+TGG 0.574824 5_1:+20797522 None:intergenic
GGTACCTTTCACGTGGCCGA+AGG 0.576737 5_1:-20793539 Msa0723190:three_prime_UTR
GCCTTATAGAAGTAGGGAGA+AGG 0.580965 5_1:-20800316 Msa0723190:CDS
TTGGCATTGTTGAGACCAGA+TGG 0.580975 5_1:-20800177 Msa0723190:CDS
CATCCTGCAGTTTACTACGA+AGG 0.583567 5_1:-20800552 Msa0723190:CDS
GCAATCAAAGTCGCCATATG+CGG 0.587969 5_1:+20797323 None:intergenic
GTGAAAAGTAGTGAATTTGG+AGG 0.590767 5_1:-20800231 Msa0723190:CDS
TGTTGATGAAAGATGGGCAA+AGG 0.591875 5_1:-20800625 Msa0723190:CDS
GAAAAGTAGTGAATTTGGAG+GGG 0.593375 5_1:-20800229 Msa0723190:CDS
TGGTGAGTTCGGTGTTTGTG+AGG 0.598575 5_1:+20797552 None:intergenic
ATTTGAGGGTGTGGGTGTAG+AGG 0.599498 5_1:+20797507 None:intergenic
GGGTGTAGAGGTAAGTGGAG+AGG 0.600636 5_1:+20797519 None:intergenic
GAAGGTGAATGGGATAAAGC+TGG 0.607431 5_1:-20800357 Msa0723190:CDS
TACTCATAGTCATTAGAACA+TGG 0.612730 5_1:+20794187 None:intergenic
TAGTGAGATGAGGAAAATTG+AGG 0.613129 5_1:-20800277 Msa0723190:CDS
GGTGTTGAGAAATCAAATGA+CGG 0.613855 5_1:-20800678 Msa0723190:CDS
ATATAAGGCTTATCTTTCGG+CGG 0.617990 5_1:+20797182 None:intergenic
GGTGCCGGATTTGAGGGTGT+GGG 0.623923 5_1:+20797499 None:intergenic
TCTGAAGGCATACTTAAGAG+AGG 0.626310 5_1:+20794220 None:intergenic
AATAGCATAATTGATAGGAG+AGG 0.629684 5_1:-20800435 Msa0723190:CDS
AAAGGGTACATATAAGGACC+TGG 0.633154 5_1:+20800150 None:intergenic
TAAGCCTTATATTTGTAACA+TGG 0.633641 5_1:-20797171 Msa0723190:CDS
TAATGGTGATGGTGAAGAAG+AGG 0.637013 5_1:-20797279 Msa0723190:CDS
TCTCTTAGGTACCTTTCACG+TGG 0.641995 5_1:-20793546 Msa0723190:three_prime_UTR
AAGGAATGGATAGTGAGATG+AGG 0.645343 5_1:-20800287 Msa0723190:CDS
AAAGAAAATAATAATGCTAG+GGG 0.649762 5_1:-20793603 Msa0723190:three_prime_UTR
TGAAGAAGAGGAAATGCAGT+TGG 0.653928 5_1:-20797267 Msa0723190:CDS
GCATCTTCTAAAGGCAAGCG+AGG 0.659909 5_1:-20794413 Msa0723190:CDS
TCTTGTTTACACTAGTGGTG+AGG 0.661471 5_1:-20800778 Msa0723190:CDS
GTGTGGTAGAAGTAGACCAA+GGG 0.671408 5_1:-20793517 Msa0723190:three_prime_UTR
ACAAGAGCACCATTGACCGA+TGG 0.679665 5_1:-20793275 Msa0723190:three_prime_UTR
GGAGATTATGAAGAAATGGG+AGG 0.680620 5_1:-20800034 Msa0723190:CDS
GGTGTGGTAGAAGTAGACCA+AGG 0.685219 5_1:-20793518 Msa0723190:three_prime_UTR
AACAGTATTTGATTGAACGG+AGG 0.685549 5_1:-20797355 Msa0723190:CDS
TGCCTGGACCTATAGACACA+TGG 0.689465 5_1:-20800071 Msa0723190:CDS
AAGAAAATAATAATGCTAGG+GGG 0.716639 5_1:-20793602 Msa0723190:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTTTAGACATTAGAATTA+TGG + chr5_1:20801022-20801041 None:intergenic 20.0%
! ATAAGGTTGAAGAATAGAAA+AGG - chr5_1:20800762-20800781 Msa0723190:CDS 25.0%
!! TCTTTTAGATTTGTCCATAA+TGG - chr5_1:20800791-20800810 Msa0723190:CDS 25.0%
!!! ATTGGTTTGGTGTATATATA+TGG - chr5_1:20800893-20800912 Msa0723190:CDS 25.0%
! AAAAAAGAGTGAACTCTATT+TGG + chr5_1:20800926-20800945 None:intergenic 25.0%
! GAATTATGGACAAATCTATT+TGG + chr5_1:20801008-20801027 None:intergenic 25.0%
! TTTGATGATCTTACAAGATG+AGG + chr5_1:20800825-20800844 None:intergenic 30.0%
! ATACTTGTGCTGATGTTAAT+TGG - chr5_1:20800875-20800894 Msa0723190:CDS 30.0%
ATAAAGGTCCTCTTCTATCA+CGG + chr5_1:20793215-20793234 None:intergenic 35.0%
AGAAGAGGACCTTTATACAA+TGG - chr5_1:20793219-20793238 Msa0723190:three_prime_UTR 35.0%
ATGAGGAACTAAGTCCATTA+TGG + chr5_1:20800808-20800827 None:intergenic 35.0%
! TGTGCTGATGTTAATTGGTT+TGG - chr5_1:20800880-20800899 Msa0723190:CDS 35.0%
! ATCTATTTGGAGCTTTCCAT+CGG + chr5_1:20800995-20801014 None:intergenic 35.0%
TTCAACCTTATCATCACCCT+TGG + chr5_1:20800753-20800772 None:intergenic 40.0%
GTCTACTTCTACCACACCTT+CGG + chr5_1:20800731-20800750 None:intergenic 45.0%
GTGTGGTAGAAGTAGACCAA+GGG - chr5_1:20800734-20800753 Msa0723190:intron 45.0%
GTAGACCAAGGGTGATGATA+AGG - chr5_1:20800745-20800764 Msa0723190:CDS 45.0%
GGTGTGGTAGAAGTAGACCA+AGG - chr5_1:20800733-20800752 Msa0723190:intron 50.0%
!! ACAAGAGCACCATTGACCGA+TGG - chr5_1:20800976-20800995 Msa0723190:intron 50.0%
TGGAGCTTTCCATCGGTCAA+TGG + chr5_1:20800988-20801007 None:intergenic 50.0%
CTTTCACGTGGCCGAAGGTG+TGG - chr5_1:20800717-20800736 Msa0723190:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 20793206 20801067 20793206 ID=Msa0723190;Name=Msa0723190
chr5_1 mRNA 20793206 20801067 20793206 ID=Msa0723190-mRNA-1;Parent=Msa0723190;Name=Msa0723190-mRNA-1;_AED=0.30;_eAED=0.31;_QI=0|0.27|0.25|1|0.09|0.08|12|772|509
chr5_1 exon 20793206 20793819 20793206 ID=Msa0723190-mRNA-1:exon:7972;Parent=Msa0723190-mRNA-1
chr5_1 exon 20793922 20794035 20793922 ID=Msa0723190-mRNA-1:exon:7971;Parent=Msa0723190-mRNA-1
chr5_1 exon 20794142 20794450 20794142 ID=Msa0723190-mRNA-1:exon:7970;Parent=Msa0723190-mRNA-1
chr5_1 exon 20797149 20797396 20797149 ID=Msa0723190-mRNA-1:exon:7969;Parent=Msa0723190-mRNA-1
chr5_1 exon 20797457 20797581 20797457 ID=Msa0723190-mRNA-1:exon:7968;Parent=Msa0723190-mRNA-1
chr5_1 exon 20798245 20798275 20798245 ID=Msa0723190-mRNA-1:exon:7967;Parent=Msa0723190-mRNA-1
chr5_1 exon 20799826 20799832 20799826 ID=Msa0723190-mRNA-1:exon:7966;Parent=Msa0723190-mRNA-1
chr5_1 exon 20800015 20800253 20800015 ID=Msa0723190-mRNA-1:exon:7965;Parent=Msa0723190-mRNA-1
chr5_1 exon 20800269 20800475 20800269 ID=Msa0723190-mRNA-1:exon:7964;Parent=Msa0723190-mRNA-1
chr5_1 exon 20800488 20800699 20800488 ID=Msa0723190-mRNA-1:exon:7963;Parent=Msa0723190-mRNA-1
chr5_1 exon 20800736 20800923 20800736 ID=Msa0723190-mRNA-1:exon:7962;Parent=Msa0723190-mRNA-1
chr5_1 exon 20801060 20801067 20801060 ID=Msa0723190-mRNA-1:exon:7961;Parent=Msa0723190-mRNA-1
chr5_1 CDS 20801060 20801067 20801060 ID=Msa0723190-mRNA-1:cds;Parent=Msa0723190-mRNA-1
chr5_1 CDS 20800736 20800923 20800736 ID=Msa0723190-mRNA-1:cds;Parent=Msa0723190-mRNA-1
chr5_1 CDS 20800488 20800699 20800488 ID=Msa0723190-mRNA-1:cds;Parent=Msa0723190-mRNA-1
chr5_1 CDS 20800269 20800475 20800269 ID=Msa0723190-mRNA-1:cds;Parent=Msa0723190-mRNA-1
chr5_1 CDS 20800015 20800253 20800015 ID=Msa0723190-mRNA-1:cds;Parent=Msa0723190-mRNA-1
chr5_1 CDS 20799826 20799832 20799826 ID=Msa0723190-mRNA-1:cds;Parent=Msa0723190-mRNA-1
chr5_1 CDS 20798245 20798275 20798245 ID=Msa0723190-mRNA-1:cds;Parent=Msa0723190-mRNA-1
chr5_1 CDS 20797457 20797581 20797457 ID=Msa0723190-mRNA-1:cds;Parent=Msa0723190-mRNA-1
chr5_1 CDS 20797149 20797396 20797149 ID=Msa0723190-mRNA-1:cds;Parent=Msa0723190-mRNA-1
chr5_1 CDS 20794186 20794450 20794186 ID=Msa0723190-mRNA-1:cds;Parent=Msa0723190-mRNA-1
chr5_1 three_prime_UTR 20794142 20794185 20794142 ID=Msa0723190-mRNA-1:three_prime_utr;Parent=Msa0723190-mRNA-1
chr5_1 three_prime_UTR 20793922 20794035 20793922 ID=Msa0723190-mRNA-1:three_prime_utr;Parent=Msa0723190-mRNA-1
chr5_1 three_prime_UTR 20793206 20793819 20793206 ID=Msa0723190-mRNA-1:three_prime_utr;Parent=Msa0723190-mRNA-1
Gene Sequence

>Msa0723190

ATGGATCCGTTTGAGCCTAACACTTTTCTTCAATTCATTGAAAACAAACATATGGCTTTTGTTGGTGATTCTATGGCTAGGAATCAGTTAGAGTCACTTCTTTGTTTGTTATCTAGTGTTTCAACACCTGATCTTGTTTACACTAGTGGTGAGGATATTAAGTTTAGAAAGTGGCATTTTGATTCTCATAATGCAAGTGTTGAGAAATCAAATGACGGGCCAAATCATAATAAGTTGTATTTGGATCTTGTTGATGAAAGATGGGCAAAGGATATGGAAAAGATGGATTTTATTGTGCTTTCAATTGGTCATTGGTTTTTGCATCCTGCAGTTTACTACGAAGGTGATTCAGTTTTAGGTTGTCATTATTGTGTTGGTCTTAATCATACTGAAATTGGGTTTTATGATGCTTTAAGAACTACCCTTAATAGCATAATTGATAGGAGAGGAAATAAAGGTAGTAAAATGGATGTACTAGTGACAACATTTTCACCAGCTCATTTTGAAGGTGAATGGGATAAAGCTGGTGCTTGTCCAAAGACTAAGCCTTATAGAAGTAGGGAGAAGGAACTTGAAGGAATGGATAGTGAGATGAGGAAAATTGAGGTTGAAGAAGTGAAAAGTAGTGAATTTGGAGGGGTTAGGTTTGAGGCACTTGATGTAACTAAATTGGCATTGTTGAGACCAGATGGTCATCCAGGTCCTTATATGTACCCTTTTCCATTTGCTAATGGGGTTCCAGAGCGTGTGCAAAATGATTGTGTTCATTGGTGTTTGCCTGGACCTATAGACACATGGAATGAGATTTTTCTGGAGATTATGAAGAAATGGGAGGACAATCCAAGAACTCAAGAAAAAGGAGTGATATATCGTTCATCAATAAGATCACCACGTAAATTCCTCACAAACACCGAACTCACCACCCTCCACCACCTCTCCACTTACCTCTACACCCACACCCTCAAATCCGGCACCGTTTGGGTTCGAGTAATGCGTGACAGCGAAGTTGACGCTATTGTGTTGAGATTTCTTGTGAAACAGTATTTGATTGAACGGAGGAGTTTGATGCCGCATATGGCGACTTTGATTGCGTTTTATAGAGGCAGTGGTGTTAATGGTGATGGTGAAGAAGAGGAAATGCAGTTGGCTGGGACTGTTGAGATTTCTTTTAATGTTTATGGTGCTAATTCTACTCTTCCGTCGCCGGATCCGCCGAAAGATAAGCCTTATATTTGTAACATGGCTGTTGATAAATCTCTTAGAAGGAGGGGCATTGGATGGCATCTTCTAAAGGCAAGCGAGGAACTGATTTCTCGGATGAGTTCATCAGGAGAAGTTTACTTGCATTGCAGAATGATTGATGAAGCTCCATTTAACATGTATACAAAAGCTGATTACAAAATTGTAACGACAGATAGTATTTTAGTCCTGCTGTTGTTGCAGAGACGCAAACACTTGATGTGCAAGAAACTTCCTCTCTTAAGTATGCCTTCAGAAACAAACGTGCCATGTTCTAATGACTATGAGTAA
Protein sequence

>Msa0723190

MDPFEPNTFLQFIENKHMAFVGDSMARNQLESLLCLLSSVSTPDLVYTSGEDIKFRKWHFDSHNASVEKSNDGPNHNKLYLDLVDERWAKDMEKMDFIVLSIGHWFLHPAVYYEGDSVLGCHYCVGLNHTEIGFYDALRTTLNSIIDRRGNKGSKMDVLVTTFSPAHFEGEWDKAGACPKTKPYRSREKELEGMDSEMRKIEVEEVKSSEFGGVRFEALDVTKLALLRPDGHPGPYMYPFPFANGVPERVQNDCVHWCLPGPIDTWNEIFLEIMKKWEDNPRTQEKGVIYRSSIRSPRKFLTNTELTTLHHLSTYLYTHTLKSGTVWVRVMRDSEVDAIVLRFLVKQYLIERRSLMPHMATLIAFYRGSGVNGDGEEEEMQLAGTVEISFNVYGANSTLPSPDPPKDKPYICNMAVDKSLRRRGIGWHLLKASEELISRMSSSGEVYLHCRMIDEAPFNMYTKADYKIVTTDSILVLLLLQRRKHLMCKKLPLLSMPSETNVPCSNDYE*