Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0724770 | XP_003613590.1 | 98.577 | 492 | 7 | 0 | 1 | 492 | 1 | 492 | 0.0 | 1010 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0724770 | sp|Q9LFP7|PBL34_ARATH | 72.267 | 494 | 107 | 8 | 16 | 488 | 4 | 488 | 0.0 | 707 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0724770 | MtrunA17_Chr5g0416011 | 98.577 | 492 | 7 | 0 | 1 | 492 | 1 | 492 | 0.0 | 1010 |
| Msa0724770 | MtrunA17_Chr8g0362661 | 85.132 | 491 | 65 | 4 | 4 | 492 | 5 | 489 | 0.0 | 831 |
| Msa0724770 | MtrunA17_Chr7g0216551 | 74.229 | 454 | 111 | 5 | 37 | 488 | 26 | 475 | 0.0 | 690 |
| Msa0724770 | MtrunA17_Chr6g0452761 | 69.600 | 500 | 122 | 6 | 4 | 490 | 3 | 485 | 0.0 | 665 |
| Msa0724770 | MtrunA17_Chr4g0064201 | 63.504 | 411 | 129 | 5 | 42 | 445 | 23 | 419 | 1.39e-180 | 513 |
| Msa0724770 | MtrunA17_Chr5g0435211 | 59.299 | 371 | 137 | 4 | 52 | 419 | 1 | 360 | 1.04e-158 | 457 |
| Msa0724770 | MtrunA17_Chr7g0238671 | 62.334 | 377 | 124 | 5 | 52 | 424 | 1 | 363 | 3.81e-155 | 447 |
| Msa0724770 | MtrunA17_Chr2g0328171 | 59.030 | 371 | 137 | 5 | 52 | 419 | 1 | 359 | 1.55e-154 | 446 |
| Msa0724770 | MtrunA17_Chr7g0245571 | 70.370 | 297 | 83 | 3 | 124 | 419 | 79 | 371 | 2.08e-151 | 439 |
| Msa0724770 | MtrunA17_Chr5g0416311 | 59.128 | 367 | 137 | 4 | 52 | 416 | 1 | 356 | 1.08e-148 | 431 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0724770 | AT5G15080.1 | 72.267 | 494 | 107 | 8 | 16 | 488 | 4 | 488 | 0.0 | 707 |
| Msa0724770 | AT3G01300.1 | 69.371 | 493 | 135 | 6 | 4 | 488 | 1 | 485 | 0.0 | 668 |
| Msa0724770 | AT3G28690.2 | 68.033 | 488 | 134 | 9 | 7 | 491 | 6 | 474 | 0.0 | 648 |
| Msa0724770 | AT3G28690.3 | 75.443 | 395 | 86 | 4 | 99 | 491 | 38 | 423 | 0.0 | 612 |
| Msa0724770 | AT3G28690.1 | 76.579 | 380 | 78 | 4 | 114 | 491 | 4 | 374 | 0.0 | 602 |
| Msa0724770 | AT1G07570.1 | 60.160 | 374 | 128 | 5 | 52 | 422 | 1 | 356 | 7.14e-157 | 452 |
| Msa0724770 | AT1G07570.5 | 60.160 | 374 | 128 | 5 | 52 | 422 | 1 | 356 | 7.14e-157 | 452 |
| Msa0724770 | AT1G07570.2 | 60.160 | 374 | 128 | 5 | 52 | 422 | 1 | 356 | 7.14e-157 | 452 |
| Msa0724770 | AT5G02290.2 | 59.030 | 371 | 131 | 4 | 52 | 419 | 1 | 353 | 5.24e-155 | 446 |
| Msa0724770 | AT5G02290.1 | 59.030 | 371 | 131 | 4 | 52 | 419 | 1 | 353 | 5.24e-155 | 446 |
Find 155 sgRNAs with CRISPR-Local
Find 330 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTGACCTTCAAGACGA+GGG | 0.693659 | 5_1:-23856426 | None:intergenic |
| AAAAGTGACGTGTACAGTTT+TGG | 0.305705 | 5_1:+23856274 | Msa0724770:CDS |
| AAAATGAAATTATCGGAGAT+GGG | 0.553291 | 5_1:-23852509 | None:intergenic |
| AAACCTTGAAGGATGGAAAA+AGG | 0.250673 | 5_1:-23852633 | None:intergenic |
| AAAGGTTGGATCGAAGAAAA+TGG | 0.302715 | 5_1:+23854459 | Msa0724770:CDS |
| AAAGTTGATAGCTCAGTCAG+TGG | 0.679259 | 5_1:+23853375 | Msa0724770:CDS |
| AACCTATATGTAAGTACGTA+AGG | 0.500790 | 5_1:-23853146 | None:intergenic |
| AACTTCACTCATTAGGGGTC+TGG | 0.443923 | 5_1:-23856512 | None:intergenic |
| AAGAAGGATAAAGAGAATGA+GGG | 0.578449 | 5_1:+23853273 | Msa0724770:CDS |
| AAGACCAAAGTCTGAGAGCT+TGG | 0.506606 | 5_1:-23855461 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAACTGAATTGAGAAATCTT+GGG | - | chr5_1:23852541-23852560 | None:intergenic | 30.0% | |
| TGAACTGAATTGAGAAATCT+TGG | - | chr5_1:23852542-23852561 | None:intergenic | 30.0% | |
| AATGAAAAGCGAAAAGGAAA+AGG | - | chr5_1:23852701-23852720 | None:intergenic | 30.0% | |
| GGTTGTGTTGTATTAATGTT+TGG | + | chr5_1:23852848-23852867 | Msa0724770:intron | 30.0% | |
| AACCTATATGTAAGTACGTA+AGG | - | chr5_1:23853149-23853168 | None:intergenic | 30.0% | |
| AAGAAGGATAAAGAGAATGA+GGG | + | chr5_1:23853273-23853292 | Msa0724770:CDS | 30.0% | |
| AAAAACAAAAACTGGGTGTT+GGG | + | chr5_1:23853311-23853330 | Msa0724770:CDS | 30.0% | |
| ATAACACATTTCCAAGGAAA+CGG | - | chr5_1:23853431-23853450 | None:intergenic | 30.0% | |
| TCAACAATAACACATTTCCA+AGG | - | chr5_1:23853437-23853456 | None:intergenic | 30.0% | |
| GATTGTTCTTATGATGTGTT+TGG | + | chr5_1:23853459-23853478 | Msa0724770:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 23852449 | 23857078 | 23852449 | ID=Msa0724770;Name=Msa0724770 |
| chr5_1 | mRNA | 23852449 | 23857078 | 23852449 | ID=Msa0724770-mRNA-1;Parent=Msa0724770;Name=Msa0724770-mRNA-1;_AED=0.16;_eAED=0.16;_QI=348|1|1|1|0.83|0.71|7|301|492 |
| chr5_1 | exon | 23852449 | 23852651 | 23852449 | ID=Msa0724770-mRNA-1:exon:8764;Parent=Msa0724770-mRNA-1 |
| chr5_1 | exon | 23853050 | 23853420 | 23853050 | ID=Msa0724770-mRNA-1:exon:8765;Parent=Msa0724770-mRNA-1 |
| chr5_1 | exon | 23854232 | 23854569 | 23854232 | ID=Msa0724770-mRNA-1:exon:8766;Parent=Msa0724770-mRNA-1 |
| chr5_1 | exon | 23854663 | 23854798 | 23854663 | ID=Msa0724770-mRNA-1:exon:8767;Parent=Msa0724770-mRNA-1 |
| chr5_1 | exon | 23855239 | 23855375 | 23855239 | ID=Msa0724770-mRNA-1:exon:8768;Parent=Msa0724770-mRNA-1 |
| chr5_1 | exon | 23855451 | 23855574 | 23855451 | ID=Msa0724770-mRNA-1:exon:8769;Parent=Msa0724770-mRNA-1 |
| chr5_1 | exon | 23856260 | 23857078 | 23856260 | ID=Msa0724770-mRNA-1:exon:8770;Parent=Msa0724770-mRNA-1 |
| chr5_1 | five_prime_UTR | 23852449 | 23852651 | 23852449 | ID=Msa0724770-mRNA-1:five_prime_utr;Parent=Msa0724770-mRNA-1 |
| chr5_1 | five_prime_UTR | 23853050 | 23853194 | 23853050 | ID=Msa0724770-mRNA-1:five_prime_utr;Parent=Msa0724770-mRNA-1 |
| chr5_1 | CDS | 23853195 | 23853420 | 23853195 | ID=Msa0724770-mRNA-1:cds;Parent=Msa0724770-mRNA-1 |
| chr5_1 | CDS | 23854232 | 23854569 | 23854232 | ID=Msa0724770-mRNA-1:cds;Parent=Msa0724770-mRNA-1 |
| chr5_1 | CDS | 23854663 | 23854798 | 23854663 | ID=Msa0724770-mRNA-1:cds;Parent=Msa0724770-mRNA-1 |
| chr5_1 | CDS | 23855239 | 23855375 | 23855239 | ID=Msa0724770-mRNA-1:cds;Parent=Msa0724770-mRNA-1 |
| chr5_1 | CDS | 23855451 | 23855574 | 23855451 | ID=Msa0724770-mRNA-1:cds;Parent=Msa0724770-mRNA-1 |
| chr5_1 | CDS | 23856260 | 23856777 | 23856260 | ID=Msa0724770-mRNA-1:cds;Parent=Msa0724770-mRNA-1 |
| chr5_1 | three_prime_UTR | 23856778 | 23857078 | 23856778 | ID=Msa0724770-mRNA-1:three_prime_utr;Parent=Msa0724770-mRNA-1 |
| Gene Sequence |
>Msa0724770 ATGGGGTTGTTGGGTTCTGAGAATGTTAAAATGGTGGAGTCTTGGGATGTGTCTAAATCAAAGGGGAGTAAGAAGATGAAGAAGGATAAAGAGAATGAGGGTGTGATTGATGATGGAAAAACAAAAACTGGGTGTTGGGTTGGGTTGAGATTCATTGGTAGCTGCATTCCTTCAAGATCCAAAGTTGATAGCTCAGTCAGTGGCAGTGGTACCAGTACTCAGTATGCTGAAAGTAAATCAACTAATGATACAAGTAGAGGCCAACGAACTGCTCCAGTAATCTCTTCTACAACCACTAGTAACGCAGAAAGCAATTCCTCAACTACTAAACTTGAAGAGGAGCTTAAAATTGCTTCCAGGCTGCGAAAATTCTCTTTCAATGAACTTAAGTTGGCAACAAGAAATTTCCGGCCCGAGAGTTTTCTTGGTGAAGGTGGCTTTGGTTGTGTTTTCAAAGGTTGGATCGAAGAAAATGGAACTGCTCCAGTGAAACCTGGCACAGGGCTTACAGTTGCTGTAAAAACCCTTAACCATGATGGGCTTCAGGGTCATAAAGAATGGCTGGCAGAGGTTAATTTTCTTGGTGATCTAGTTCATCAAAACCTTGTTAAACTCATAGGTTACTGCATTGAAGATGATCAAAGGCTGCTAGTGTACGAGTTCATGCCTCGGGGAAGCCTGGAAAATCACTTATTTAGGAGATCCATGCCTCTTCCATGGTCCATTAGGATGAAAATTGCACTAGGAGCTGCTAAGGGTCTTGCTTTTCTTCACGAAGAAGCTGAACGACCAGTAATATATAGGGATTTTAAAACTTCGAATATACTATTAGATGCAGACTACAATGCCAAGCTCTCAGACTTTGGTCTTGCCAAAGATGGTCCAGAAGGTGATAAAACCCACGTGTCTACTCGGGTGATGGGAACATATGGTTATGCAGCACCAGAATATGTCATGACAGGTCATCTTACGTCAAAAAGTGACGTGTACAGTTTTGGAGTCGTACTACTTGAGATGATATCTGGCAGAAGATCTATGGACAAACACCGACCCAACGGCGAACATAACCTTGTCGAATGGGCTCGACCACATCTAGGAGAGCGAAGAAGGTTCTACCGGTTGATAGACCCTCGTCTTGAAGGTCACTTTTCCGTAAAAGGAGCTCAGAAAGCCGCTCAGCTAGCTCATCATTGCCTAAGCAGAGATCCAAAAGCCAGACCCCTAATGAGTGAAGTTGTCGAAGCTTTAATGCCTTTACCAAACCTAAAGGACATGGCAAGCTCTTCGTACTATTTCCAATCAATGCAAGCCGAGCGGTTCGGAGCGAGTCCAAATTCTAGAAGTATGCATGCACAAGGAGCATCGCTTGCTCGGAACGGACAGCAGAGAAGGAGCCTTTCGATACCGAATGGTGCACATGTATCTCCTTATCACCATAAGTATCCTCAACAATCACCTAAACCTAATGTCAAAGCATAG |
| Protein sequence |
>Msa0724770 MGLLGSENVKMVESWDVSKSKGSKKMKKDKENEGVIDDGKTKTGCWVGLRFIGSCIPSRSKVDSSVSGSGTSTQYAESKSTNDTSRGQRTAPVISSTTTSNAESNSSTTKLEEELKIASRLRKFSFNELKLATRNFRPESFLGEGGFGCVFKGWIEENGTAPVKPGTGLTVAVKTLNHDGLQGHKEWLAEVNFLGDLVHQNLVKLIGYCIEDDQRLLVYEFMPRGSLENHLFRRSMPLPWSIRMKIALGAAKGLAFLHEEAERPVIYRDFKTSNILLDADYNAKLSDFGLAKDGPEGDKTHVSTRVMGTYGYAAPEYVMTGHLTSKSDVYSFGVVLLEMISGRRSMDKHRPNGEHNLVEWARPHLGERRRFYRLIDPRLEGHFSVKGAQKAAQLAHHCLSRDPKARPLMSEVVEALMPLPNLKDMASSSYYFQSMQAERFGASPNSRSMHAQGASLARNGQQRRSLSIPNGAHVSPYHHKYPQQSPKPNVKA* |
