AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0725210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0725210 MtrunA17_Chr7g0231611 78.250 400 33 2 1 400 1 346 0.0 619
Msa0725210 MtrunA17_Chr5g0416851 78.500 400 32 2 1 400 1 346 0.0 602
Msa0725210 MtrunA17_Chr2g0282701 69.565 299 50 3 102 400 20 277 2.28e-132 380
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 107 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATCTTGCATTTCTTAAA+TGG 0.169370 5_1:+24776528 Msa0725210:three_prime_UTR
CTTTGATGCTGTTATGGTTT+TGG 0.176146 5_1:+24776493 Msa0725210:three_prime_UTR
ATATATTACCCAAACGAAAT+TGG 0.204217 5_1:+24772975 Msa0725210:five_prime_UTR
ACAATTATTCTATTGGATTT+TGG 0.205917 5_1:+24776262 Msa0725210:three_prime_UTR
TTATAAATCACGCCAATATT+TGG 0.228451 5_1:-24775685 None:intergenic
TTTCTCTTGCATTGATTCAT+AGG 0.243951 5_1:+24773000 Msa0725210:five_prime_UTR
GGTGTAGGGTTCATAGGATT+TGG 0.270592 5_1:+24776327 Msa0725210:three_prime_UTR
GTGTAGTGGTGGATCGTATT+GGG 0.274886 5_1:-24774119 None:intergenic
ATCCTCTACAATCTTCTTCT+TGG 0.277613 5_1:-24776085 None:intergenic
GGCCTTCGGAATGTTTAAAT+TGG 0.278518 5_1:+24776351 Msa0725210:three_prime_UTR
GCTGTTTACAATTATTCTAT+TGG 0.290906 5_1:+24776255 Msa0725210:three_prime_UTR
TGATTGATAACCCTCAATTA+TGG 0.292414 5_1:-24775201 None:intergenic
TATATTACCCAAACGAAATT+GGG 0.296600 5_1:+24772976 Msa0725210:five_prime_UTR
GTGATTTATAATGGTGAAAA+TGG 0.304153 5_1:+24775697 Msa0725210:CDS
GTGATTTCTAATGGTGAAAA+TGG 0.304848 5_1:+24775907 Msa0725210:CDS
CGGTTGGATTTCCACACAAT+TGG 0.311637 5_1:+24775503 Msa0725210:CDS
AGTGGAGATCTAACACTATT+TGG 0.318692 5_1:-24776003 None:intergenic
AAGCTATTACTTGCAGCTTC+AGG 0.320186 5_1:-24775155 None:intergenic
GAAGCAGACTCTAAAACTTA+TGG 0.323607 5_1:-24776392 None:intergenic
TGTGATCTTTGATGCTGTTA+TGG 0.324694 5_1:+24776487 Msa0725210:three_prime_UTR
TTCAATTTCTTTGCGTAAAG+TGG 0.326249 5_1:-24776021 None:intergenic
CTTCTCACCTTCCCTATTGT+AGG 0.331736 5_1:-24774022 None:intergenic
AATATTGGCGTGATTTATAA+TGG 0.332979 5_1:+24775688 Msa0725210:CDS
TACCTGTGTTATCGTGGTTT+CGG 0.335763 5_1:-24773040 None:intergenic
AGCTTGTCTCAACCAAATAT+TGG 0.339420 5_1:+24775673 Msa0725210:CDS
TGCAAGGGTTTAGCTCTTGC+TGG 0.346892 5_1:+24774309 Msa0725210:CDS
GGTGTAGTGGTGGATCGTAT+TGG 0.352995 5_1:-24774120 None:intergenic
GCCTCACGTACGTCTCAAAA+TGG 0.354055 5_1:+24774535 Msa0725210:CDS
AAGTTACACGATGCTTAAAA+AGG 0.366432 5_1:+24776158 Msa0725210:CDS
AGAGAAACCCCAATTTCGTT+TGG 0.373457 5_1:-24772984 None:intergenic
ACAGGTATGTCGCCGTTTCT+CGG 0.373889 5_1:+24775483 Msa0725210:intron
GCTAAACCCTTGCACTGGTT+TGG 0.374373 5_1:-24774300 None:intergenic
TATATAGGTGTAGGGTTCAT+AGG 0.374473 5_1:+24776321 Msa0725210:three_prime_UTR
AATTATTCAGCTCATAGTTC+AGG 0.376079 5_1:+24775529 Msa0725210:CDS
GTCCTAACTGAACAATTAGT+TGG 0.387724 5_1:+24776284 Msa0725210:three_prime_UTR
TGAAACTTTGCAGGAAGATA+TGG 0.390389 5_1:+24775771 Msa0725210:CDS
GAAAACGTGGATAAAGTTAC+TGG 0.399696 5_1:+24775562 Msa0725210:CDS
TCTTGCTGGCATTGCTTCAA+TGG 0.402134 5_1:+24774323 Msa0725210:CDS
AGCAGCCGATGAAACTTTGC+AGG 0.410172 5_1:+24775762 Msa0725210:CDS
GAGAAACCCCAATTTCGTTT+GGG 0.413354 5_1:-24772983 None:intergenic
GCAATGTTGTCATTGCCTTC+CGG 0.413401 5_1:-24775853 None:intergenic
GGTGGATCGTATTGGGGTCT+TGG 0.417450 5_1:-24774112 None:intergenic
TTTCGTTTGGGTAATATATG+TGG 0.422223 5_1:-24772971 None:intergenic
CTGGTAACTTGTGTAGTAGT+AGG 0.423638 5_1:+24776109 Msa0725210:CDS
TCATAGGATTTGGAGGCCTT+CGG 0.428488 5_1:+24776337 Msa0725210:three_prime_UTR
CAGGTTGACGTGATTTCTAA+TGG 0.433158 5_1:+24775898 Msa0725210:CDS
GAAGGTAATGATAACATTGC+GGG 0.433461 5_1:+24775799 Msa0725210:CDS
GGGTCTTAAGTTGTCTCAAC+AGG 0.433791 5_1:+24775819 Msa0725210:CDS
AAGAAGATTGTAGAGGATAC+TGG 0.442437 5_1:+24776090 Msa0725210:CDS
ATCTTGAGTCTCTACAACAT+TGG 0.448912 5_1:-24774479 None:intergenic
TTCTCTTGCATTGATTCATA+GGG 0.451642 5_1:+24773001 Msa0725210:five_prime_UTR
GCAATGTTATCATTACCTTC+AGG 0.456016 5_1:-24775796 None:intergenic
TTTAGATTTGAAGACTGAGT+TGG 0.457380 5_1:-24774148 None:intergenic
GTTGTCTCAACAGGAAGATA+TGG 0.459418 5_1:+24775828 Msa0725210:CDS
TCTTGAGTCTCTACAACATT+GGG 0.460912 5_1:-24774478 None:intergenic
AGCCTGGTAGATCTTCTAAC+AGG 0.463228 5_1:-24776213 None:intergenic
TTTGAAGACTGAGTTGGAGA+GGG 0.464825 5_1:-24774142 None:intergenic
GTATGTCGCCGTTTCTCGGT+TGG 0.465222 5_1:+24775487 Msa0725210:CDS
AATGTTTAAATTGGTGCCTC+AGG 0.472565 5_1:+24776360 Msa0725210:three_prime_UTR
AAACCCTTGCACTGGTTTGG+AGG 0.480121 5_1:-24774297 None:intergenic
AGCCAACTAATTGTTCAGTT+AGG 0.480363 5_1:-24776286 None:intergenic
TTCGTTTGGGTAATATATGT+GGG 0.482927 5_1:-24772970 None:intergenic
ATCTAACACTATTTGGCTCA+TGG 0.496988 5_1:-24775996 None:intergenic
TTTGAAGATATATAGGTGTA+GGG 0.500420 5_1:+24776313 Msa0725210:three_prime_UTR
GTGGAAATCCAACCGAGAAA+CGG 0.509162 5_1:-24775495 None:intergenic
ATCTTCCTGCAAAGTTTCAT+CGG 0.511056 5_1:-24775767 None:intergenic
TGAGTTGGAGAGGGGTGTAG+TGG 0.518631 5_1:-24774133 None:intergenic
TTGAAGACTGAGTTGGAGAG+GGG 0.520107 5_1:-24774141 None:intergenic
GTTGGAGAGGGGTGTAGTGG+TGG 0.540155 5_1:-24774130 None:intergenic
TCTAACACTATTTGGCTCAT+GGG 0.544732 5_1:-24775995 None:intergenic
CTGTGTTATCGTGGTTTCGG+AGG 0.545214 5_1:-24773037 None:intergenic
GTAGGGTTCATAGGATTTGG+AGG 0.548528 5_1:+24776330 Msa0725210:three_prime_UTR
TGGTAACTTGTGTAGTAGTA+GGG 0.548652 5_1:+24776110 Msa0725210:CDS
ACCCTCAATTATGGTTTGTG+TGG 0.551749 5_1:-24775192 None:intergenic
ATTTGAAGACTGAGTTGGAG+AGG 0.552156 5_1:-24774143 None:intergenic
CTCCACACAAACCATAATTG+AGG 0.558366 5_1:+24775190 Msa0725210:CDS
GAAATGCAAGATTGAAATAG+CGG 0.558749 5_1:-24776520 None:intergenic
TCCACACAAACCATAATTGA+GGG 0.566292 5_1:+24775191 Msa0725210:CDS
TCAACAGGAAGATATGGATC+CGG 0.567658 5_1:+24775834 Msa0725210:CDS
CAAGAGCTAAACCCTTGCAC+TGG 0.575418 5_1:-24774305 None:intergenic
GAGAATTACCTGTGTTATCG+TGG 0.576982 5_1:-24773046 None:intergenic
AACAGTTGACGAGACTTCAC+AGG 0.579898 5_1:+24775618 Msa0725210:CDS
GAAACCTCCAAACCAGTGCA+AGG 0.581895 5_1:+24774293 Msa0725210:CDS
CTCCTGTTAGAAGATCTACC+AGG 0.582686 5_1:+24776211 Msa0725210:CDS
TGTAGTGGTGGATCGTATTG+GGG 0.583890 5_1:-24774118 None:intergenic
ACACTATTTGGCTCATGGGT+AGG 0.583997 5_1:-24775991 None:intergenic
CGAATTATCAAGTGACCGCA+AGG 0.593011 5_1:+24776050 Msa0725210:CDS
ATATTACCCAAACGAAATTG+GGG 0.594702 5_1:+24772977 Msa0725210:five_prime_UTR
CACCAATTTAAACATTCCGA+AGG 0.601638 5_1:-24776353 None:intergenic
CGTTGACAAGAAAATCAGAA+TGG 0.605012 5_1:+24774086 Msa0725210:exon
TGGTACGTTTCTTATCCTTG+CGG 0.610799 5_1:-24776065 None:intergenic
GGCATCACTGTAATCTTCCG+CGG 0.614423 5_1:+24774502 Msa0725210:CDS
TCGTTTGGGTAATATATGTG+GGG 0.620057 5_1:-24772969 None:intergenic
CAGGAAGATATGGAACCTGA+AGG 0.622434 5_1:+24775781 Msa0725210:CDS
AAACCTCCAAACCAGTGCAA+GGG 0.640873 5_1:+24774294 Msa0725210:CDS
CTCCGAAACCACGATAACAC+AGG 0.642190 5_1:+24773038 Msa0725210:five_prime_UTR
AGGAAATGAATGTGAAAACG+TGG 0.652661 5_1:+24775549 Msa0725210:CDS
GAGACTTCACAGGAAGCTGA+AGG 0.656001 5_1:+24775628 Msa0725210:CDS
AATGTTGTAGAGACTCAAGA+TGG 0.656225 5_1:+24774481 Msa0725210:CDS
CAGGAAGATATGGATCCGGA+AGG 0.665840 5_1:+24775838 Msa0725210:CDS
TGAAGGTAATGATAACATTG+CGG 0.667655 5_1:+24775798 Msa0725210:CDS
TACCAAGAAGAAGATTGTAG+AGG 0.672640 5_1:+24776083 Msa0725210:CDS
TGTTCTAGGGTCATCCACAG+TGG 0.677614 5_1:+24775120 Msa0725210:intron
TTAGAAATCACGTCAACCTG+CGG 0.710726 5_1:-24775895 None:intergenic
CTTATGGAATATGATTCCTG+AGG 0.718569 5_1:-24776376 None:intergenic
GGTTCAAGTTCAATCCACTG+TGG 0.730886 5_1:-24775134 None:intergenic
GTGAGGCGTTAATCAAACCG+CGG 0.757884 5_1:-24774519 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAATCTTATAATTTGTT+TGG - chr5_1:24773947-24773966 None:intergenic 10.0%
!!! AATTTATTTAAAATCAATGT+CGG - chr5_1:24774699-24774718 None:intergenic 10.0%
!!! TTTTGATAAAATAGTAAATT+TGG - chr5_1:24775085-24775104 None:intergenic 10.0%
!!! TGTAACTTTTTTTTTAAACT+CGG - chr5_1:24773483-24773502 None:intergenic 15.0%
!!! AATTGTTAAAGCTTTTTTTT+GGG + chr5_1:24773730-24773749 Msa0725210:intron 15.0%
!! TTAATTATGAAATGAAAAAG+TGG + chr5_1:24773973-24773992 Msa0725210:intron 15.0%
!!! TTTTTTTCATTTTCATTTGT+AGG + chr5_1:24774353-24774372 Msa0725210:intron 15.0%
!!! CAATTGTTAAAGCTTTTTTT+TGG + chr5_1:24773729-24773748 Msa0725210:intron 20.0%
!! TAATTACTTATTGTGACTAT+TGG + chr5_1:24774400-24774419 Msa0725210:intron 20.0%
!! TTGAATATAATTAGTGTGTT+TGG + chr5_1:24774653-24774672 Msa0725210:intron 20.0%
!! AACAAAGTTAGCAAAAATAT+AGG - chr5_1:24775255-24775274 None:intergenic 20.0%
!!! TTTTTTATAATTTAGTAGCC+TGG - chr5_1:24776232-24776251 None:intergenic 20.0%
!!! ACAATTATTCTATTGGATTT+TGG + chr5_1:24776262-24776281 Msa0725210:three_prime_UTR 20.0%
! ATATATTACCCAAACGAAAT+TGG + chr5_1:24772975-24772994 Msa0725210:five_prime_UTR 25.0%
! TATATTACCCAAACGAAATT+GGG + chr5_1:24772976-24772995 Msa0725210:five_prime_UTR 25.0%
! AGTGCATAAAAAAAAGAACT+GGG - chr5_1:24773130-24773149 None:intergenic 25.0%
! AAGTGCATAAAAAAAAGAAC+TGG - chr5_1:24773131-24773150 None:intergenic 25.0%
! TTTACAGTCACAAATATAAC+AGG - chr5_1:24773244-24773263 None:intergenic 25.0%
!!! TTTTTTTTAAACTCGGTATC+CGG - chr5_1:24773476-24773495 None:intergenic 25.0%
! ACAAAATTAAAACATAGTGC+TGG - chr5_1:24773509-24773528 None:intergenic 25.0%
! AATAGGAACAAATGAGATAA+TGG - chr5_1:24773625-24773644 None:intergenic 25.0%
! ATTACGAAACTCAAAGAAAT+AGG - chr5_1:24773642-24773661 None:intergenic 25.0%
! ATAGTATTAGACACAAATCA+TGG - chr5_1:24773690-24773709 None:intergenic 25.0%
! TTATTTCACACAGATTATGA+AGG - chr5_1:24773864-24773883 None:intergenic 25.0%
! ATATGTCAATTATTGTGAGA+TGG + chr5_1:24773888-24773907 Msa0725210:intron 25.0%
!! TGTAGATTTTCTTACATGAA+TGG + chr5_1:24774262-24774281 Msa0725210:intron 25.0%
! TTCATTTGTAGGTTTCTAAA+TGG + chr5_1:24774364-24774383 Msa0725210:intron 25.0%
! AGAGGAATGAAAAAGAAAAA+GGG - chr5_1:24774582-24774601 None:intergenic 25.0%
! AAGAGGAATGAAAAAGAAAA+AGG - chr5_1:24774583-24774602 None:intergenic 25.0%
!! TTGAGAGTACAAATCAAATT+TGG + chr5_1:24774795-24774814 Msa0725210:intron 25.0%
!!! TTTAGATTCACTTTTAAGAG+AGG - chr5_1:24774935-24774954 None:intergenic 25.0%
!!! ATCAATCATTCAGTTTTATG+TGG + chr5_1:24775216-24775235 Msa0725210:CDS 25.0%
!!! TGTCAAAAAGTACAGATTTT+GGG - chr5_1:24775341-24775360 None:intergenic 25.0%
!!! TTGTCAAAAAGTACAGATTT+TGG - chr5_1:24775342-24775361 None:intergenic 25.0%
! AAATTAAATGCGTCAATAAC+AGG - chr5_1:24775439-24775458 None:intergenic 25.0%
!!! TATTGACGCATTTAATTTTC+TGG + chr5_1:24775441-24775460 Msa0725210:intron 25.0%
! AATATTCAATGTCTTTCAAC+AGG + chr5_1:24775465-24775484 Msa0725210:intron 25.0%
!! GAATAATTTTTCCAATTGTG+TGG - chr5_1:24775517-24775536 None:intergenic 25.0%
! TTATAAATCACGCCAATATT+TGG - chr5_1:24775688-24775707 None:intergenic 25.0%
! AATATTGGCGTGATTTATAA+TGG + chr5_1:24775688-24775707 Msa0725210:CDS 25.0%
! GTGATTTATAATGGTGAAAA+TGG + chr5_1:24775697-24775716 Msa0725210:CDS 25.0%
! GCTGTTTACAATTATTCTAT+TGG + chr5_1:24776255-24776274 Msa0725210:three_prime_UTR 25.0%
!!! TTTTGAAGATATATAGGTGT+AGG + chr5_1:24776312-24776331 Msa0725210:three_prime_UTR 25.0%
! TTTGAAGATATATAGGTGTA+GGG + chr5_1:24776313-24776332 Msa0725210:three_prime_UTR 25.0%
!!! TTTATTTGTACCTCCTTTTT+TGG + chr5_1:24776416-24776435 Msa0725210:three_prime_UTR 25.0%
!!! TTATTTGTACCTCCTTTTTT+GGG + chr5_1:24776417-24776436 Msa0725210:three_prime_UTR 25.0%
! TTAATTCAACAACCCAAAAA+AGG - chr5_1:24776432-24776451 None:intergenic 25.0%
!!! TTTTTTGGGTTGTTGAATTA+AGG + chr5_1:24776431-24776450 Msa0725210:three_prime_UTR 25.0%
!!! TTTTTGGGTTGTTGAATTAA+GGG + chr5_1:24776432-24776451 Msa0725210:three_prime_UTR 25.0%
! TCAATCTTGCATTTCTTAAA+TGG + chr5_1:24776528-24776547 Msa0725210:three_prime_UTR 25.0%
TTCGTTTGGGTAATATATGT+GGG - chr5_1:24772973-24772992 None:intergenic 30.0%
TTTCGTTTGGGTAATATATG+TGG - chr5_1:24772974-24772993 None:intergenic 30.0%
ATATTACCCAAACGAAATTG+GGG + chr5_1:24772977-24772996 Msa0725210:five_prime_UTR 30.0%
!! TTTCTCTTGCATTGATTCAT+AGG + chr5_1:24773000-24773019 Msa0725210:five_prime_UTR 30.0%
!! TTCTCTTGCATTGATTCATA+GGG + chr5_1:24773001-24773020 Msa0725210:five_prime_UTR 30.0%
TCAGTTCAAACAAAAGACAT+GGG - chr5_1:24773093-24773112 None:intergenic 30.0%
ATCAGTTCAAACAAAAGACA+TGG - chr5_1:24773094-24773113 None:intergenic 30.0%
AAAAAAAAGAACTGGGAATC+TGG - chr5_1:24773123-24773142 None:intergenic 30.0%
GAATTTGAACAAAGTCTATG+AGG - chr5_1:24773172-24773191 None:intergenic 30.0%
! ATACTTCCAATTTTCTGCAA+AGG + chr5_1:24773280-24773299 Msa0725210:intron 30.0%
ATTCTGATTCTCTAGTTAGT+GGG + chr5_1:24773543-24773562 Msa0725210:intron 30.0%
! ACACAAATCATGGTGTTTTA+CGG - chr5_1:24773680-24773699 None:intergenic 30.0%
AAAAGTGGTTGAACATGTTT+AGG + chr5_1:24773988-24774007 Msa0725210:intron 30.0%
! AGCAAAGTTTTCCTACAATA+GGG + chr5_1:24774011-24774030 Msa0725210:five_prime_UTR 30.0%
TTTAGATTTGAAGACTGAGT+TGG - chr5_1:24774151-24774170 None:intergenic 30.0%
ATCTACAAAAACAAGCAAGT+AGG - chr5_1:24774249-24774268 None:intergenic 30.0%
! AGATTTTCTTACATGAATGG+TGG + chr5_1:24774265-24774284 Msa0725210:intron 30.0%
! TCAAAATGGTTTTTCTTCTG+AGG + chr5_1:24774549-24774568 Msa0725210:CDS 30.0%
! TTTTTGCGTCAAACTTTCTT+TGG - chr5_1:24775037-24775056 None:intergenic 30.0%
! TGATTGATAACCCTCAATTA+TGG - chr5_1:24775204-24775223 None:intergenic 30.0%
AAAATACACATAATCCTCAC+TGG + chr5_1:24775383-24775402 Msa0725210:intron 30.0%
AATTATTCAGCTCATAGTTC+AGG + chr5_1:24775529-24775548 Msa0725210:CDS 30.0%
TGAAGGTAATGATAACATTG+CGG + chr5_1:24775798-24775817 Msa0725210:CDS 30.0%
GTGATTTCTAATGGTGAAAA+TGG + chr5_1:24775907-24775926 Msa0725210:CDS 30.0%
TTCAATTTCTTTGCGTAAAG+TGG - chr5_1:24776024-24776043 None:intergenic 30.0%
AAGTTACACGATGCTTAAAA+AGG + chr5_1:24776158-24776177 Msa0725210:CDS 30.0%
!! GCTTGCTTTTGAAGATATAT+AGG + chr5_1:24776306-24776325 Msa0725210:three_prime_UTR 30.0%
! TTTGATGCTGTTATGGTTTT+GGG + chr5_1:24776494-24776513 Msa0725210:three_prime_UTR 30.0%
GAAATGCAAGATTGAAATAG+CGG - chr5_1:24776523-24776542 None:intergenic 30.0%
AACTCTCCTTTGCAGAAAAT+TGG - chr5_1:24773289-24773308 None:intergenic 35.0%
! ATTTGCAACCCATTTGAGTT+TGG + chr5_1:24773326-24773345 Msa0725210:intron 35.0%
GTCTCAGGTTTGATTCTATT+CGG + chr5_1:24773393-24773412 Msa0725210:intron 35.0%
! TCATTTTCACAAGTGGTTTG+TGG + chr5_1:24773418-24773437 Msa0725210:intron 35.0%
!! CATTTTCACAAGTGGTTTGT+GGG + chr5_1:24773419-24773438 Msa0725210:intron 35.0%
GATTCTGATTCTCTAGTTAG+TGG + chr5_1:24773542-24773561 Msa0725210:intron 35.0%
TGTCAATTATTGTGAGATGG+CGG + chr5_1:24773891-24773910 Msa0725210:intron 35.0%
! GAGCAAAGTTTTCCTACAAT+AGG + chr5_1:24774010-24774029 Msa0725210:five_prime_UTR 35.0%
! AAGTTTTCCTACAATAGGGA+AGG + chr5_1:24774015-24774034 Msa0725210:five_prime_UTR 35.0%
CGTTGACAAGAAAATCAGAA+TGG + chr5_1:24774086-24774105 Msa0725210:exon 35.0%
AACAAGAGAGAAGAGTGAAT+GGG - chr5_1:24774183-24774202 None:intergenic 35.0%
AAACAAGAGAGAAGAGTGAA+TGG - chr5_1:24774184-24774203 None:intergenic 35.0%
TCTTGAGTCTCTACAACATT+GGG - chr5_1:24774481-24774500 None:intergenic 35.0%
ATCTTGAGTCTCTACAACAT+TGG - chr5_1:24774482-24774501 None:intergenic 35.0%
AATGTTGTAGAGACTCAAGA+TGG + chr5_1:24774481-24774500 Msa0725210:CDS 35.0%
!! AAATGGTTTTTCTTCTGAGG+TGG + chr5_1:24774552-24774571 Msa0725210:intron 35.0%
!!! AATGGTTTTTCTTCTGAGGT+GGG + chr5_1:24774553-24774572 Msa0725210:intron 35.0%
! CGAGTTAGAATTCATTTTCG+TGG + chr5_1:24774843-24774862 Msa0725210:intron 35.0%
AACATGTCAACATTAGTGTC+TGG + chr5_1:24774896-24774915 Msa0725210:intron 35.0%
!! TAGTGTCTGGAATCACTTTT+AGG + chr5_1:24774909-24774928 Msa0725210:intron 35.0%
TGTGTTTGCTTCATGTTCTA+GGG + chr5_1:24775107-24775126 Msa0725210:intron 35.0%
TCCACACAAACCATAATTGA+GGG + chr5_1:24775191-24775210 Msa0725210:CDS 35.0%
! TAGACATGAGATTTAGAGTC+TGG + chr5_1:24775296-24775315 Msa0725210:intron 35.0%
GGTATTTATTCAATCCAGTG+AGG - chr5_1:24775400-24775419 None:intergenic 35.0%
AGGAAATGAATGTGAAAACG+TGG + chr5_1:24775549-24775568 Msa0725210:CDS 35.0%
GAAAACGTGGATAAAGTTAC+TGG + chr5_1:24775562-24775581 Msa0725210:CDS 35.0%
!!! TCTCGTCAACTGTTTTTTTG+TGG - chr5_1:24775612-24775631 None:intergenic 35.0%
AGCTTGTCTCAACCAAATAT+TGG + chr5_1:24775673-24775692 Msa0725210:CDS 35.0%
ATCTTCCTGCAAAGTTTCAT+CGG - chr5_1:24775770-24775789 None:intergenic 35.0%
TGAAACTTTGCAGGAAGATA+TGG + chr5_1:24775771-24775790 Msa0725210:CDS 35.0%
GCAATGTTATCATTACCTTC+AGG - chr5_1:24775799-24775818 None:intergenic 35.0%
GAAGGTAATGATAACATTGC+GGG + chr5_1:24775799-24775818 Msa0725210:CDS 35.0%
! TCTAACACTATTTGGCTCAT+GGG - chr5_1:24775998-24776017 None:intergenic 35.0%
! ATCTAACACTATTTGGCTCA+TGG - chr5_1:24775999-24776018 None:intergenic 35.0%
AGTGGAGATCTAACACTATT+TGG - chr5_1:24776006-24776025 None:intergenic 35.0%
TACCAAGAAGAAGATTGTAG+AGG + chr5_1:24776083-24776102 Msa0725210:CDS 35.0%
ATCCTCTACAATCTTCTTCT+TGG - chr5_1:24776088-24776107 None:intergenic 35.0%
AAGAAGATTGTAGAGGATAC+TGG + chr5_1:24776090-24776109 Msa0725210:CDS 35.0%
! TGGTAACTTGTGTAGTAGTA+GGG + chr5_1:24776110-24776129 Msa0725210:CDS 35.0%
!! AAGCATCGTGTAACTTTTCT+TGG - chr5_1:24776154-24776173 None:intergenic 35.0%
GTCCTAACTGAACAATTAGT+TGG + chr5_1:24776284-24776303 Msa0725210:three_prime_UTR 35.0%
AGCCAACTAATTGTTCAGTT+AGG - chr5_1:24776289-24776308 None:intergenic 35.0%
TATATAGGTGTAGGGTTCAT+AGG + chr5_1:24776321-24776340 Msa0725210:three_prime_UTR 35.0%
CACCAATTTAAACATTCCGA+AGG - chr5_1:24776356-24776375 None:intergenic 35.0%
! AATGTTTAAATTGGTGCCTC+AGG + chr5_1:24776360-24776379 Msa0725210:three_prime_UTR 35.0%
CTTATGGAATATGATTCCTG+AGG - chr5_1:24776379-24776398 None:intergenic 35.0%
!! GAAGCAGACTCTAAAACTTA+TGG - chr5_1:24776395-24776414 None:intergenic 35.0%
ATTCAACAACCCAAAAAAGG+AGG - chr5_1:24776429-24776448 None:intergenic 35.0%
TGTGATCTTTGATGCTGTTA+TGG + chr5_1:24776487-24776506 Msa0725210:three_prime_UTR 35.0%
! CTTTGATGCTGTTATGGTTT+TGG + chr5_1:24776493-24776512 Msa0725210:three_prime_UTR 35.0%
GAGAAACCCCAATTTCGTTT+GGG - chr5_1:24772986-24773005 None:intergenic 40.0%
AGAGAAACCCCAATTTCGTT+TGG - chr5_1:24772987-24773006 None:intergenic 40.0%
TACCTGTGTTATCGTGGTTT+CGG - chr5_1:24773043-24773062 None:intergenic 40.0%
GAGAATTACCTGTGTTATCG+TGG - chr5_1:24773049-24773068 None:intergenic 40.0%
!!! TTCGGTGTCATTTTCACAAG+TGG + chr5_1:24773411-24773430 Msa0725210:intron 40.0%
!! CACAGATTATGAAGGCATCA+TGG - chr5_1:24773856-24773875 None:intergenic 40.0%
GTCAATTATTGTGAGATGGC+GGG + chr5_1:24773892-24773911 Msa0725210:intron 40.0%
! TTTGAAGACTGAGTTGGAGA+GGG - chr5_1:24774145-24774164 None:intergenic 40.0%
! ATTTGAAGACTGAGTTGGAG+AGG - chr5_1:24774146-24774165 None:intergenic 40.0%
ATTCCTCTTGACCCTTATCT+AGG + chr5_1:24774594-24774613 Msa0725210:intron 40.0%
AATACCACGAGCCTAGATAA+GGG - chr5_1:24774608-24774627 None:intergenic 40.0%
AAATACCACGAGCCTAGATA+AGG - chr5_1:24774609-24774628 None:intergenic 40.0%
GTGTGTTTGCTTCATGTTCT+AGG + chr5_1:24775106-24775125 Msa0725210:intron 40.0%
AAGCTATTACTTGCAGCTTC+AGG - chr5_1:24775158-24775177 None:intergenic 40.0%
CTCCACACAAACCATAATTG+AGG + chr5_1:24775190-24775209 Msa0725210:CDS 40.0%
! ACCCTCAATTATGGTTTGTG+TGG - chr5_1:24775195-24775214 None:intergenic 40.0%
!!! TTCATCGGCTGCTTTTTTGA+GGG - chr5_1:24775755-24775774 None:intergenic 40.0%
!!! TTTCATCGGCTGCTTTTTTG+AGG - chr5_1:24775756-24775775 None:intergenic 40.0%
GTTGTCTCAACAGGAAGATA+TGG + chr5_1:24775828-24775847 Msa0725210:CDS 40.0%
TCAACAGGAAGATATGGATC+CGG + chr5_1:24775834-24775853 Msa0725210:CDS 40.0%
!!! TTTTGATTTGTCTCAACCGC+AGG + chr5_1:24775879-24775898 Msa0725210:CDS 40.0%
TTAGAAATCACGTCAACCTG+CGG - chr5_1:24775898-24775917 None:intergenic 40.0%
! CAGGTTGACGTGATTTCTAA+TGG + chr5_1:24775898-24775917 Msa0725210:CDS 40.0%
TGGTACGTTTCTTATCCTTG+CGG - chr5_1:24776068-24776087 None:intergenic 40.0%
! CTGGTAACTTGTGTAGTAGT+AGG + chr5_1:24776109-24776128 Msa0725210:CDS 40.0%
GGCCTTCGGAATGTTTAAAT+TGG + chr5_1:24776351-24776370 Msa0725210:three_prime_UTR 40.0%
ATCAAACCTGAGACCTTGAG+AGG - chr5_1:24773387-24773406 None:intergenic 45.0%
ATTAGACCCCTCAGATTCGT+CGG + chr5_1:24773442-24773461 Msa0725210:intron 45.0%
CTTCTCACCTTCCCTATTGT+AGG - chr5_1:24774025-24774044 None:intergenic 45.0%
!! TGTAGTGGTGGATCGTATTG+GGG - chr5_1:24774121-24774140 None:intergenic 45.0%
!! GTGTAGTGGTGGATCGTATT+GGG - chr5_1:24774122-24774141 None:intergenic 45.0%
! TTGAAGACTGAGTTGGAGAG+GGG - chr5_1:24774144-24774163 None:intergenic 45.0%
AAACCTCCAAACCAGTGCAA+GGG + chr5_1:24774294-24774313 Msa0725210:CDS 45.0%
TCTTGCTGGCATTGCTTCAA+TGG + chr5_1:24774323-24774342 Msa0725210:CDS 45.0%
TTGTGACTATTGGTGCATGC+AGG + chr5_1:24774410-24774429 Msa0725210:intron 45.0%
!!! ACCATTTTGAGACGTACGTG+AGG - chr5_1:24774539-24774558 None:intergenic 45.0%
! GGTTCAAGTTCAATCCACTG+TGG - chr5_1:24775137-24775156 None:intergenic 45.0%
! GTCTGGCAATTTTGTCTGAC+AGG + chr5_1:24775313-24775332 Msa0725210:intron 45.0%
GTGGAAATCCAACCGAGAAA+CGG - chr5_1:24775498-24775517 None:intergenic 45.0%
CGGTTGGATTTCCACACAAT+TGG + chr5_1:24775503-24775522 Msa0725210:CDS 45.0%
AACAGTTGACGAGACTTCAC+AGG + chr5_1:24775618-24775637 Msa0725210:CDS 45.0%
CAGGAAGATATGGAACCTGA+AGG + chr5_1:24775781-24775800 Msa0725210:CDS 45.0%
! GGGTCTTAAGTTGTCTCAAC+AGG + chr5_1:24775819-24775838 Msa0725210:CDS 45.0%
GCAATGTTGTCATTGCCTTC+CGG - chr5_1:24775856-24775875 None:intergenic 45.0%
! ACACTATTTGGCTCATGGGT+AGG - chr5_1:24775994-24776013 None:intergenic 45.0%
CGAATTATCAAGTGACCGCA+AGG + chr5_1:24776050-24776069 Msa0725210:CDS 45.0%
CTCCTGTTAGAAGATCTACC+AGG + chr5_1:24776211-24776230 Msa0725210:CDS 45.0%
AGCCTGGTAGATCTTCTAAC+AGG - chr5_1:24776216-24776235 None:intergenic 45.0%
GGTGTAGGGTTCATAGGATT+TGG + chr5_1:24776327-24776346 Msa0725210:three_prime_UTR 45.0%
GTAGGGTTCATAGGATTTGG+AGG + chr5_1:24776330-24776349 Msa0725210:three_prime_UTR 45.0%
TCATAGGATTTGGAGGCCTT+CGG + chr5_1:24776337-24776356 Msa0725210:three_prime_UTR 45.0%
!!! TTAAATTTTAGAATTAATTT+TGG + chr5_1:24774962-24774981 Msa0725210:intron 5.0%
CTGTGTTATCGTGGTTTCGG+AGG - chr5_1:24773040-24773059 None:intergenic 50.0%
CTCCGAAACCACGATAACAC+AGG + chr5_1:24773038-24773057 Msa0725210:five_prime_UTR 50.0%
! CAACCCATTTGAGTTTGGCC+CGG + chr5_1:24773331-24773350 Msa0725210:intron 50.0%
CTACCGGGCCAAACTCAAAT+GGG - chr5_1:24773337-24773356 None:intergenic 50.0%
ACTACCGGGCCAAACTCAAA+TGG - chr5_1:24773338-24773357 None:intergenic 50.0%
AGGTCCCAAGCTAACACTAC+CGG - chr5_1:24773353-24773372 None:intergenic 50.0%
! GTAGTGTTAGCTTGGGACCT+TGG + chr5_1:24773353-24773372 Msa0725210:intron 50.0%
CACATACCGACGAATCTGAG+GGG - chr5_1:24773451-24773470 None:intergenic 50.0%
CCACATACCGACGAATCTGA+GGG - chr5_1:24773452-24773471 None:intergenic 50.0%
CCTCAGATTCGTCGGTATGT+GGG + chr5_1:24773450-24773469 Msa0725210:intron 50.0%
!! GGTGTAGTGGTGGATCGTAT+TGG - chr5_1:24774123-24774142 None:intergenic 50.0%
GAAACCTCCAAACCAGTGCA+AGG + chr5_1:24774293-24774312 Msa0725210:CDS 50.0%
!! AAACCCTTGCACTGGTTTGG+AGG - chr5_1:24774300-24774319 None:intergenic 50.0%
!! GCTAAACCCTTGCACTGGTT+TGG - chr5_1:24774303-24774322 None:intergenic 50.0%
CAAGAGCTAAACCCTTGCAC+TGG - chr5_1:24774308-24774327 None:intergenic 50.0%
!! TGCAAGGGTTTAGCTCTTGC+TGG + chr5_1:24774309-24774328 Msa0725210:CDS 50.0%
GGCATCACTGTAATCTTCCG+CGG + chr5_1:24774502-24774521 Msa0725210:CDS 50.0%
! GTGAGGCGTTAATCAAACCG+CGG - chr5_1:24774522-24774541 None:intergenic 50.0%
GCCTCACGTACGTCTCAAAA+TGG + chr5_1:24774535-24774554 Msa0725210:CDS 50.0%
GAGCCTAGATAAGGGTCAAG+AGG - chr5_1:24774600-24774619 None:intergenic 50.0%
TTGACCCTTATCTAGGCTCG+TGG + chr5_1:24774601-24774620 Msa0725210:intron 50.0%
!! TGTTCTAGGGTCATCCACAG+TGG + chr5_1:24775120-24775139 Msa0725210:intron 50.0%
ACAGGCGCAATCACACACAA+GGG - chr5_1:24775421-24775440 None:intergenic 50.0%
AACAGGCGCAATCACACACA+AGG - chr5_1:24775422-24775441 None:intergenic 50.0%
ACAGGTATGTCGCCGTTTCT+CGG + chr5_1:24775483-24775502 Msa0725210:intron 50.0%
GAGACTTCACAGGAAGCTGA+AGG + chr5_1:24775628-24775647 Msa0725210:CDS 50.0%
AGCAGCCGATGAAACTTTGC+AGG + chr5_1:24775762-24775781 Msa0725210:CDS 50.0%
CAGGAAGATATGGATCCGGA+AGG + chr5_1:24775838-24775857 Msa0725210:CDS 50.0%
TTGGCCCGGTAGTGTTAGCT+TGG + chr5_1:24773345-24773364 Msa0725210:intron 55.0%
! TGGCCCGGTAGTGTTAGCTT+GGG + chr5_1:24773346-24773365 Msa0725210:intron 55.0%
GGTCCCAAGCTAACACTACC+GGG - chr5_1:24773352-24773371 None:intergenic 55.0%
CCCTCAGATTCGTCGGTATG+TGG + chr5_1:24773449-24773468 Msa0725210:intron 55.0%
CCCACATACCGACGAATCTG+AGG - chr5_1:24773453-24773472 None:intergenic 55.0%
AGATTCGTCGGTATGTGGGC+CGG + chr5_1:24773454-24773473 Msa0725210:intron 55.0%
GGTGGATCGTATTGGGGTCT+TGG - chr5_1:24774115-24774134 None:intergenic 55.0%
! TGAGTTGGAGAGGGGTGTAG+TGG - chr5_1:24774136-24774155 None:intergenic 55.0%
GTATGTCGCCGTTTCTCGGT+TGG + chr5_1:24775487-24775506 Msa0725210:CDS 55.0%
! CTTGAGAGGAGCACGCTCCA+AGG - chr5_1:24773373-24773392 None:intergenic 60.0%
CTTGGAGCGTGCTCCTCTCA+AGG + chr5_1:24773371-24773390 Msa0725210:intron 60.0%
CGTGCTCCTCTCAAGGTCTC+AGG + chr5_1:24773378-24773397 Msa0725210:intron 60.0%
! GTTGGAGAGGGGTGTAGTGG+TGG - chr5_1:24774133-24774152 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 24772970 24776581 24772970 ID=Msa0725210;Name=Msa0725210
chr5_1 mRNA 24772970 24776581 24772970 ID=Msa0725210-mRNA-1;Parent=Msa0725210;Name=Msa0725210-mRNA-1;_AED=0.12;_eAED=0.15;_QI=185|0.5|0.71|0.85|0.83|0.85|7|327|400
chr5_1 exon 24772970 24773059 24772970 ID=Msa0725210-mRNA-1:exon:8996;Parent=Msa0725210-mRNA-1
chr5_1 exon 24774010 24774173 24774010 ID=Msa0725210-mRNA-1:exon:8997;Parent=Msa0725210-mRNA-1
chr5_1 exon 24774267 24774346 24774267 ID=Msa0725210-mRNA-1:exon:8998;Parent=Msa0725210-mRNA-1
chr5_1 exon 24774432 24774570 24774432 ID=Msa0725210-mRNA-1:exon:8999;Parent=Msa0725210-mRNA-1
chr5_1 exon 24775128 24775237 24775128 ID=Msa0725210-mRNA-1:exon:9000;Parent=Msa0725210-mRNA-1
chr5_1 exon 24775335 24775371 24775335 ID=Msa0725210-mRNA-1:exon:9001;Parent=Msa0725210-mRNA-1
chr5_1 exon 24775487 24776581 24775487 ID=Msa0725210-mRNA-1:exon:9002;Parent=Msa0725210-mRNA-1
chr5_1 five_prime_UTR 24772970 24773059 24772970 ID=Msa0725210-mRNA-1:five_prime_utr;Parent=Msa0725210-mRNA-1
chr5_1 five_prime_UTR 24774010 24774104 24774010 ID=Msa0725210-mRNA-1:five_prime_utr;Parent=Msa0725210-mRNA-1
chr5_1 CDS 24774105 24774173 24774105 ID=Msa0725210-mRNA-1:cds;Parent=Msa0725210-mRNA-1
chr5_1 CDS 24774267 24774346 24774267 ID=Msa0725210-mRNA-1:cds;Parent=Msa0725210-mRNA-1
chr5_1 CDS 24774432 24774570 24774432 ID=Msa0725210-mRNA-1:cds;Parent=Msa0725210-mRNA-1
chr5_1 CDS 24775128 24775237 24775128 ID=Msa0725210-mRNA-1:cds;Parent=Msa0725210-mRNA-1
chr5_1 CDS 24775335 24775371 24775335 ID=Msa0725210-mRNA-1:cds;Parent=Msa0725210-mRNA-1
chr5_1 CDS 24775487 24776254 24775487 ID=Msa0725210-mRNA-1:cds;Parent=Msa0725210-mRNA-1
chr5_1 three_prime_UTR 24776255 24776581 24776255 ID=Msa0725210-mRNA-1:three_prime_utr;Parent=Msa0725210-mRNA-1
Gene Sequence

>Msa0725210

ATGGCTTCCAAGACCCCAATACGATCCACCACTACACCCCTCTCCAACTCAGTCTTCAAATCTAAACACATTTTCTTACATGAATGGTGGCTTGTGAAACCTCCAAACCAGTGCAAGGGTTTAGCTCTTGCTGGCATTGCTTCAATGGAGAGAGAAAGAATGTTTTTTTCTTCAGTGATTGTAGAGAGACATGAACCCAATGTTGTAGAGACTCAAGATGGCATCACTGTAATCTTCCGCGGTTTGATTAACGCCTCACGTACGTCTCAAAATGGTTTTTCTTCTGAGGGTCATCCACAGTGGATTGAACTTGAACCTGAAGCTGCAAGTAATAGCTTCAAACACACTGACTCCACACAAACCATAATTGAGGGTTATCAATCATTCAGTTTTATGTGGAACCCAAAATCTGTACTTTTTGACAAAATCAATTCTGTATGTCGCCGTTTCTCGGTTGGATTTCCACACAATTGGAAAAATTATTCAGCTCATAGTTCAGGAAATGAATGTGAAAACGTGGATAAAGTTACTGGTTTTGACGACTCAAATACTTCTTTCCACAAAAAAACAGTTGACGAGACTTCACAGGAAGCTGAAGGCAACGACAATATTGCGAATCTTAGCTTGTCTCAACCAAATATTGGCGTGATTTATAATGGTGAAAATGGATTCTCAATTGTTGACAACTCAAATGCTTCATCCCTCAAAAAAGCAGCCGATGAAACTTTGCAGGAAGATATGGAACCTGAAGGTAATGATAACATTGCGGGTCTTAAGTTGTCTCAACAGGAAGATATGGATCCGGAAGGCAATGACAACATTGCGAGTTTTGATTTGTCTCAACCGCAGGTTGACGTGATTTCTAATGGTGAAAATGGAGTCTCAAGTGTTGCTGCAGCTGAAAATAGTCAATCTTTGATGAGTGTATTTGAATTTGATCCTACCCATGAGCCAAATAGTGTTAGATCTCCACTTTACGCAAAGAAATTGAAGTTTGACGAATTATCAAGTGACCGCAAGGATAAGAAACGTACCAAGAAGAAGATTGTAGAGGATACTGGTAACTTGTGTAGTAGTAGGGTGCTGACAAGAAGCATTGCCAAGAAAAGTTACACGATGCTTAAAAAGGATAGAAAAACTGCAGTGAAATGTTTCACTTCTCCTGTTAGAAGATCTACCAGGCTACTAAATTATAAAAAATGA
Protein sequence

>Msa0725210

MASKTPIRSTTTPLSNSVFKSKHIFLHEWWLVKPPNQCKGLALAGIASMERERMFFSSVIVERHEPNVVETQDGITVIFRGLINASRTSQNGFSSEGHPQWIELEPEAASNSFKHTDSTQTIIEGYQSFSFMWNPKSVLFDKINSVCRRFSVGFPHNWKNYSAHSSGNECENVDKVTGFDDSNTSFHKKTVDETSQEAEGNDNIANLSLSQPNIGVIYNGENGFSIVDNSNASSLKKAADETLQEDMEPEGNDNIAGLKLSQQEDMDPEGNDNIASFDLSQPQVDVISNGENGVSSVAAAENSQSLMSVFEFDPTHEPNSVRSPLYAKKLKFDELSSDRKDKKRTKKKIVEDTGNLCSSRVLTRSIAKKSYTMLKKDRKTAVKCFTSPVRRSTRLLNYKK*