AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0725630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0725630 MtrunA17_Chr5g0417281 89.354 526 54 2 1 526 1 524 0.0 952
Msa0725630 MtrunA17_Chr5g0436761 53.592 515 176 7 1 510 1 457 1.72e-175 502
Msa0725630 MtrunA17_Chr5g0417211 77.287 317 66 3 1 313 1 315 1.69e-174 498
Msa0725630 MtrunA17_Chr5g0417211 60.000 55 21 1 464 517 352 406 6.50e-11 64.3
Msa0725630 MtrunA17_Chr5g0424931 49.548 553 212 10 1 518 8 528 2.18e-163 474
Msa0725630 MtrunA17_Chr2g0289221 70.159 315 89 4 1 312 9 321 8.36e-151 436
Msa0725630 MtrunA17_Chr2g0277461 46.628 519 195 11 4 514 5 449 7.98e-133 393
Msa0725630 MtrunA17_Chr3g0115271 41.115 574 264 13 2 521 9 562 1.00e-123 375
Msa0725630 MtrunA17_Chr1g0178011 50.685 365 163 9 1 354 1 359 1.86e-110 336
Msa0725630 MtrunA17_Chr1g0178011 48.718 78 40 0 426 503 359 436 4.96e-13 71.2
Msa0725630 MtrunA17_Chr5g0424741 40.971 515 230 13 1 448 1 508 3.27e-103 320
Msa0725630 MtrunA17_Chr5g0424801 47.989 348 152 9 1 332 1 335 5.49e-99 304
Msa0725630 MtrunA17_Chr5g0425261 48.276 348 151 9 1 332 1 335 1.18e-97 300
Msa0725630 MtrunA17_Chr2g0326761 35.870 552 254 16 9 494 8 525 3.49e-90 291
Msa0725630 MtrunA17_Chr4g0028971 37.083 480 207 12 1 414 1 451 2.26e-87 283
Msa0725630 MtrunA17_Chr2g0277531 46.006 313 155 10 6 312 14 318 1.15e-85 267
Msa0725630 MtrunA17_Chr1g0181211 39.518 415 180 9 2 353 16 422 2.90e-83 272
Msa0725630 MtrunA17_Chr1g0199681 38.557 402 174 9 9 351 91 478 5.24e-79 261
Msa0725630 MtrunA17_Chr7g0264171 33.512 561 266 18 9 495 28 555 7.20e-75 248
Msa0725630 MtrunA17_Chr5g0425661 53.093 194 87 2 12 204 37 227 4.19e-65 211
Msa0725630 MtrunA17_Chr7g0264161 38.907 311 159 8 22 312 2 301 6.93e-64 210
Msa0725630 MtrunA17_Chr4g0009751 33.854 384 201 9 12 359 19 385 7.89e-60 208
Msa0725630 MtrunA17_Chr2g0282961 34.493 345 192 8 12 332 53 387 3.77e-59 206
Msa0725630 MtrunA17_Chr4g0046371 34.091 352 188 9 12 332 38 376 2.67e-58 205
Msa0725630 MtrunA17_Chr2g0276781 36.034 358 206 8 12 353 23 373 3.77e-58 204
Msa0725630 MtrunA17_Chr8g0346711 35.659 387 209 12 2 360 14 388 1.51e-57 203
Msa0725630 MtrunA17_Chr7g0239061 34.375 352 205 8 1 334 3 346 1.98e-57 202
Msa0725630 MtrunA17_Chr2g0285461 34.545 385 215 12 9 368 20 392 8.46e-57 204
Msa0725630 MtrunA17_Chr5g0433301 33.509 379 219 11 12 367 24 392 3.84e-55 198
Msa0725630 MtrunA17_Chr8g0376771 33.862 378 226 10 9 368 20 391 4.96e-55 199
Msa0725630 MtrunA17_Chr8g0385791 34.254 362 212 8 12 354 59 413 1.48e-54 197
Msa0725630 MtrunA17_Chr1g0156321 32.553 470 263 17 12 448 43 491 1.72e-54 197
Msa0725630 MtrunA17_Chr2g0301251 34.085 355 200 7 9 340 20 363 1.24e-53 196
Msa0725630 MtrunA17_Chr3g0109391 33.161 386 211 12 12 367 26 394 2.33e-53 193
Msa0725630 MtrunA17_Chr4g0070181 34.000 350 211 6 9 342 20 365 4.21e-53 194
Msa0725630 MtrunA17_Chr2g0326281 34.456 386 196 15 12 352 56 429 8.60e-53 192
Msa0725630 MtrunA17_Chr8g0389081 35.311 354 189 10 9 335 29 369 8.60e-52 190
Msa0725630 MtrunA17_Chr4g0029671 34.294 347 198 10 12 332 54 396 1.73e-49 182
Msa0725630 MtrunA17_Chr5g0424791 43.243 148 74 4 63 210 14 151 1.38e-27 108
Msa0725630 MtrunA17_Chr5g0425651 50.000 120 53 5 231 344 2 120 2.71e-26 103
Msa0725630 MtrunA17_Chr8g0356211 27.434 226 148 6 122 335 2 223 7.64e-25 105
Msa0725630 MtrunA17_Chr5g0419861 42.478 113 58 3 53 162 15 123 6.96e-18 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0725630 AT1G77850.1 36.822 535 270 11 9 494 16 531 7.08e-107 331
Msa0725630 AT1G77850.2 36.822 535 270 11 9 494 16 531 8.33e-107 332
Msa0725630 AT2G28350.2 39.655 406 181 9 4 351 2 401 1.16e-84 276
Msa0725630 AT2G28350.1 39.655 406 181 9 4 351 2 401 1.55e-84 276
Msa0725630 AT4G30080.1 36.784 454 217 9 9 411 16 450 1.97e-84 275
Msa0725630 AT4G23980.1 37.982 337 187 8 12 332 12 342 1.18e-63 219
Msa0725630 AT4G23980.2 37.982 337 187 8 12 332 12 342 1.26e-63 219
Msa0725630 AT2G33860.1 36.261 353 185 8 12 337 54 393 1.98e-63 218
Msa0725630 AT2G46530.1 36.111 360 204 10 12 355 21 370 1.88e-58 204
Msa0725630 AT5G37020.2 33.687 377 219 10 12 366 22 389 2.20e-58 207
Msa0725630 AT5G37020.1 33.687 377 219 10 12 366 22 389 3.62e-58 207
Msa0725630 AT2G46530.3 36.111 360 204 10 12 355 42 391 4.27e-58 204
Msa0725630 AT3G61830.1 35.097 359 209 9 12 353 25 376 9.34e-58 202
Msa0725630 AT1G59750.1 34.734 357 212 7 12 352 22 373 1.00e-56 201
Msa0725630 AT1G59750.3 34.734 357 212 7 12 352 22 373 1.00e-56 201
Msa0725630 AT1G59750.2 34.734 357 212 7 12 352 22 373 1.02e-56 201
Msa0725630 AT1G59750.4 34.734 357 212 7 12 352 22 373 1.11e-56 201
Msa0725630 AT5G60450.2 36.538 364 209 9 7 349 36 398 1.76e-56 202
Msa0725630 AT1G35520.1 35.457 361 205 9 12 355 27 376 2.53e-56 198
Msa0725630 AT1G19850.1 36.011 361 190 12 9 342 51 397 3.58e-56 202
Msa0725630 AT1G19850.2 36.011 361 190 12 9 342 40 386 3.82e-56 202
Msa0725630 AT5G60450.1 36.769 359 205 9 12 349 65 422 4.87e-56 201
Msa0725630 AT1G30330.3 34.711 363 204 11 12 351 25 377 1.74e-54 197
Msa0725630 AT1G30330.2 34.711 363 204 11 12 351 25 377 1.74e-54 197
Msa0725630 AT1G30330.1 34.711 363 204 11 12 351 23 375 2.21e-54 197
Msa0725630 AT5G62000.4 34.332 367 205 10 12 354 61 415 7.52e-54 195
Msa0725630 AT5G62000.2 33.696 368 206 10 12 354 61 415 8.23e-54 195
Msa0725630 AT5G62000.5 33.696 368 206 10 12 354 61 415 8.23e-54 195
Msa0725630 AT5G62000.3 33.696 368 206 10 12 354 61 415 8.23e-54 195
Msa0725630 AT5G62000.1 33.696 368 206 10 12 354 61 415 8.23e-54 195
Msa0725630 AT1G34410.1 35.693 339 191 8 12 334 27 354 1.02e-53 191
Msa0725630 AT1G35540.1 35.294 340 193 8 12 335 27 355 1.64e-53 191
Msa0725630 AT1G35240.1 35.693 339 191 8 12 334 27 354 3.56e-53 190
Msa0725630 AT1G34390.1 33.973 365 203 11 12 355 27 374 5.76e-53 189
Msa0725630 AT1G34310.1 35.652 345 193 9 7 334 22 354 6.92e-53 189
Msa0725630 AT1G19220.1 33.978 362 195 10 9 342 20 365 1.17e-51 190
Msa0725630 AT5G20730.3 33.333 357 205 7 9 342 21 367 3.72e-50 186
Msa0725630 AT5G20730.1 33.333 357 205 7 9 342 21 367 3.87e-50 186
Msa0725630 AT5G20730.2 33.613 357 203 8 9 342 21 366 1.16e-49 184
Msa0725630 AT2G33860.2 39.076 238 128 4 114 337 2 236 4.90e-49 176
Msa0725630 AT3G61830.2 34.951 309 180 8 59 353 24 325 5.47e-46 169
Msa0725630 AT1G34170.1 30.523 344 204 9 12 334 25 354 1.13e-44 165
Msa0725630 AT5G37020.3 34.328 268 157 7 114 366 31 294 1.76e-42 162
Msa0725630 AT2G46530.2 37.209 258 141 8 111 355 34 283 4.78e-40 152
Msa0725630 AT1G34170.2 30.952 336 187 11 12 334 25 328 1.84e-38 147
Msa0725630 AT1G34170.3 31.250 336 186 11 12 334 25 328 3.84e-38 147
Msa0725630 AT1G43950.1 36.364 209 113 5 12 213 27 222 1.23e-33 127

Find 132 sgRNAs with CRISPR-Local

Find 346 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATGTTTAAACTCTATATT+TGG 0.091140 5_1:+25357003 None:intergenic
CCTGGTTCATTTATGCTATT+TGG 0.118465 5_1:-25356691 Msa0725630:CDS
TCTCAATATCATAGGTTAAA+TGG 0.119810 5_1:-25357221 Msa0725630:intron
GCACAGCACTGCCAGATTTC+TGG 0.173902 5_1:+25358717 None:intergenic
TGCTGCATTCAGTGATAAAA+AGG 0.230634 5_1:-25357070 Msa0725630:CDS
CAATCTTGGTCCTATATCTT+TGG 0.252865 5_1:-25356800 Msa0725630:CDS
GGGAGAGTGTGTTAGGGTTT+GGG 0.258688 5_1:+25358613 None:intergenic
CGTGTTTACTACTTCCCATT+AGG 0.273970 5_1:-25358660 Msa0725630:CDS
CCTAACACACTCTCCCTTCT+TGG 0.290201 5_1:-25358606 Msa0725630:CDS
CCAAGAAGGGAGAGTGTGTT+AGG 0.303749 5_1:+25358606 None:intergenic
GTGATATTCACGGCAAAGTT+TGG 0.308610 5_1:-25358278 Msa0725630:CDS
ATGTGTTCTAAGTGCCCTAA+TGG 0.313804 5_1:+25358645 None:intergenic
TTTGATGCCTTCAATTTCAT+TGG 0.329868 5_1:+25356597 None:intergenic
TGAACCAGGACTAACTTTCT+TGG 0.334964 5_1:+25356705 None:intergenic
GATAAAAGCAATGGTTTCCT+TGG 0.335382 5_1:-25356868 Msa0725630:CDS
TCTGGAACGTGCGAGACTTT+AGG 0.336417 5_1:+25357192 None:intergenic
TAAACACGAGAATATAGTTT+TGG 0.340637 5_1:+25358675 None:intergenic
TGAGTCTTTCATGAAAATTA+AGG 0.342069 5_1:+25358158 None:intergenic
GATATAGGACCAAGATTGTT+TGG 0.344613 5_1:+25356805 None:intergenic
GATTTCTGGCTCAACACGGC+TGG 0.344702 5_1:+25358731 None:intergenic
AGGGAGAGTGTGTTAGGGTT+TGG 0.345088 5_1:+25358612 None:intergenic
ATTGTTCAACAATGTTTGAT+TGG 0.354882 5_1:+25356966 None:intergenic
GTTTACGGAGGCATTGGAAT+TGG 0.359403 5_1:-25358064 Msa0725630:CDS
ATGAATATTAAGTGGAATCT+TGG 0.367936 5_1:-25357949 Msa0725630:CDS
CTCAATATCATAGGTTAAAT+GGG 0.383997 5_1:-25357220 Msa0725630:intron
GCCGTGTTGAGCCAGAAATC+TGG 0.395763 5_1:-25358728 Msa0725630:CDS
GTGTTTACTACTTCCCATTA+GGG 0.402184 5_1:-25358659 Msa0725630:CDS
GAAATCTGGCAGTGCTGTGC+AGG 0.404779 5_1:-25358714 Msa0725630:CDS
AATGTTTAAACTCTATATTT+GGG 0.405482 5_1:+25357004 None:intergenic
AAAGAGATGCTCAAAATTTG+AGG 0.405775 5_1:-25357887 Msa0725630:CDS
GTTCGCCGACAGACAAAGTT+TGG 0.410958 5_1:-25358114 Msa0725630:CDS
AGCATCCGCCATGGACGATT+AGG 0.415227 5_1:+25357841 None:intergenic
CATTGCATCCTCCACAACTT+TGG 0.422061 5_1:+25357969 None:intergenic
TTCCAGAGAGAGTCAGTCCT+TGG 0.427770 5_1:-25357176 Msa0725630:CDS
TCTTGAAAATCGCTTCCAAC+AGG 0.432199 5_1:+25356655 None:intergenic
TCCCAAGGACTGACTCTCTC+TGG 0.440968 5_1:+25357174 None:intergenic
ATTAATTTCGCGCATTCCAC+TGG 0.445870 5_1:+25358354 None:intergenic
TCTCTTGTGACAAAGTTTGC+CGG 0.446910 5_1:-25356745 Msa0725630:CDS
AGACCAAGATGATGGCAAAA+AGG 0.450916 5_1:-25358439 Msa0725630:CDS
GTGTATTATCCGATGGCTAA+AGG 0.458436 5_1:-25358015 Msa0725630:CDS
GGAGAGTGTGTTAGGGTTTG+GGG 0.461223 5_1:+25358614 None:intergenic
GCTCTTCTTGATTGCATGCA+GGG 0.464239 5_1:-25356940 Msa0725630:CDS
GACATCTGCTTACCACTGAT+TGG 0.464434 5_1:-25358221 Msa0725630:CDS
ATAAAAGCAATGGTTTCCTT+GGG 0.470549 5_1:-25356867 Msa0725630:CDS
TCAAGTGAGCCAAACAATCT+TGG 0.472055 5_1:-25356814 Msa0725630:CDS
GGTGACAAGTCCAAAGATAT+AGG 0.472899 5_1:+25356790 None:intergenic
TGCTCTTCTTGATTGCATGC+AGG 0.478114 5_1:-25356941 Msa0725630:CDS
AAAATCGTTTACGGAGGCAT+TGG 0.479022 5_1:-25358070 Msa0725630:CDS
TCCAGAGAGAGTCAGTCCTT+GGG 0.479886 5_1:-25357175 Msa0725630:CDS
AAAGCTTAGAAAATCTGATC+AGG 0.481737 5_1:-25357097 Msa0725630:CDS
TGGGGTGAGAGTCTTGGTAT+AGG 0.489342 5_1:+25358410 None:intergenic
CTCCTTCGTCCCAAATATAG+AGG 0.491263 5_1:-25357043 Msa0725630:CDS
TTGATTGCATGCAGGGAGCC+CGG 0.491918 5_1:-25356933 Msa0725630:CDS
TCTTGGTCTTCACGAACCTC+AGG 0.497877 5_1:+25358453 None:intergenic
AGTGTGTTAGGGTTTGGGGA+TGG 0.498039 5_1:+25358618 None:intergenic
CTCACTCCTGTCACTAATAA+CGG 0.499710 5_1:-25358495 Msa0725630:CDS
TGTGTTCTAAGTGCCCTAAT+GGG 0.500812 5_1:+25358646 None:intergenic
TACGGAGGCATTGGAATTGG+CGG 0.504623 5_1:-25358061 Msa0725630:CDS
CAAGAGAGTTAGAAATATCA+TGG 0.505101 5_1:+25356761 None:intergenic
ATTTCTGGCTCAACACGGCT+GGG 0.505787 5_1:+25358732 None:intergenic
CCGCCATTGTTAGCATCAGA+TGG 0.508974 5_1:+25358390 None:intergenic
TCACGAACCTCAGGAGATTG+AGG 0.509135 5_1:+25358462 None:intergenic
CGCCATTGTTAGCATCAGAT+GGG 0.516466 5_1:+25358391 None:intergenic
GAGACGGTGCATAGAATGAA+TGG 0.520972 5_1:+25358573 None:intergenic
AAAGGCTCCAATGAAATTGA+AGG 0.522242 5_1:-25356604 Msa0725630:CDS
TGAACACCGTTATTAGTGAC+AGG 0.525054 5_1:+25358489 None:intergenic
GGTAAGATCATAAAACCTGT+TGG 0.525681 5_1:-25356670 Msa0725630:CDS
CAGCATTGAGGTCAAGTGGC+GGG 0.528362 5_1:+25358328 None:intergenic
TTTATGAATGATAAAAGCAA+TGG 0.529057 5_1:-25356877 Msa0725630:CDS
CTCTCCATCAGTGATATTCA+CGG 0.529787 5_1:-25358288 Msa0725630:CDS
GCAGTGCTGTGCAGGCAGTT+CGG 0.530524 5_1:-25358706 Msa0725630:CDS
GGTGTTGCTGTTGTCAGTAC+CGG 0.533496 5_1:+25356726 None:intergenic
AATATTAAGTGGAATCTTGG+AGG 0.534264 5_1:-25357946 Msa0725630:CDS
ATCAGATGGGGTGAGAGTCT+TGG 0.534822 5_1:+25358404 None:intergenic
TGAAGTTGTGTATTATCCGA+TGG 0.535100 5_1:-25358022 Msa0725630:CDS
AAGAGATGCTCAAAATTTGA+GGG 0.539733 5_1:-25357886 Msa0725630:CDS
ATGGGAAATGATGATTGTAA+AGG 0.540341 5_1:-25356622 Msa0725630:CDS
GTTCAACAATGTTTGATTGG+AGG 0.545186 5_1:+25356969 None:intergenic
TCGTCCATGGCGGATGCTTG+AGG 0.546119 5_1:-25357836 Msa0725630:intron
TGGATACAGTGCAAATATGT+CGG 0.548922 5_1:+25357137 None:intergenic
AGGATGCAATGAATATTAAG+TGG 0.550257 5_1:-25357957 Msa0725630:CDS
ATGGACGGGAACTGCCAAGA+AGG 0.550509 5_1:+25358592 None:intergenic
CAAGAAGGGAGAGTGTGTTA+GGG 0.557064 5_1:+25358607 None:intergenic
GTTGACAAGAAGAAACTAGT+TGG 0.559606 5_1:-25358189 Msa0725630:CDS
TTTCCTTGGGAACAATTCTA+CGG 0.560425 5_1:-25356854 Msa0725630:CDS
TTTCAAGAAGTAGATATCAT+GGG 0.561761 5_1:-25356640 Msa0725630:CDS
CGGTGCATTCTCCGTGCCAG+TGG 0.572523 5_1:-25358370 Msa0725630:CDS
AGTGGTAAGCAGATGTCGCA+AGG 0.572865 5_1:+25358227 None:intergenic
AGCCTCTATATTTGGGACGA+AGG 0.575856 5_1:+25357041 None:intergenic
GCTGGGTTGCTGAGAAGACA+TGG 0.576060 5_1:+25358749 None:intergenic
GAGTAGGTCAACCGCGGAGA+CGG 0.578786 5_1:+25358557 None:intergenic
TAGTTTGACGAATACTTCAT+CGG 0.580000 5_1:+25358521 None:intergenic
GTTCGTGAAGACCAAGATGA+TGG 0.580300 5_1:-25358447 Msa0725630:CDS
TATCTGCTCCTAATCGTCCA+TGG 0.582308 5_1:-25357849 Msa0725630:CDS
AAAGTTGGAAGAAGTTGATG+TGG 0.582671 5_1:+25356897 None:intergenic
AGGGAGTCCCACGGTAGACA+TGG 0.583730 5_1:+25358247 None:intergenic
TGGACGGGAACTGCCAAGAA+GGG 0.586243 5_1:+25358593 None:intergenic
TGTTGTTGATGCCAAAGTTG+TGG 0.589052 5_1:-25357980 Msa0725630:CDS
TTACACCAACCTTTAGCCAT+CGG 0.591900 5_1:+25358006 None:intergenic
GGAATTGGCGGATAAGAACA+TGG 0.591988 5_1:-25358049 Msa0725630:CDS
TTAGAAATATCATGGCTATG+AGG 0.592683 5_1:+25356769 None:intergenic
AACACCAAGAAAGTTAGTCC+TGG 0.601578 5_1:-25356709 Msa0725630:CDS
CAGCACCAAACTTTGTCTGT+CGG 0.605931 5_1:+25358109 None:intergenic
GAAATAGTTTCAACCTCCCA+AGG 0.609478 5_1:+25357159 None:intergenic
TTTGCCGTGAATATCACTGA+TGG 0.609703 5_1:+25358284 None:intergenic
CCAAATAGCATAAATGAACC+AGG 0.612115 5_1:+25356691 None:intergenic
AGAGAGAGTCAGTCCTTGGG+AGG 0.615146 5_1:-25357172 Msa0725630:CDS
ATATTAAGTGGAATCTTGGA+GGG 0.616357 5_1:-25357945 Msa0725630:CDS
ATTATCCGATGGCTAAAGGT+TGG 0.620292 5_1:-25358011 Msa0725630:CDS
CATTCTATGCACCGTCTCCG+CGG 0.621676 5_1:-25358568 Msa0725630:CDS
TCAGACGGAAATATCTCAGT+CGG 0.627485 5_1:-25358138 Msa0725630:CDS
GACAAGAAGAAACTAGTTGG+CGG 0.628517 5_1:-25358186 Msa0725630:CDS
CGGTGCATAGAATGAATGGA+CGG 0.632419 5_1:+25358577 None:intergenic
TATACCTCAAGCATCCGCCA+TGG 0.633522 5_1:+25357832 None:intergenic
GTGCCGTAGAATTGTTCCCA+AGG 0.635548 5_1:+25356851 None:intergenic
TGTTGATGCCAAAGTTGTGG+AGG 0.640943 5_1:-25357977 Msa0725630:CDS
AACGAACTCACTCCAATCAG+TGG 0.642790 5_1:+25358209 None:intergenic
GGTGCATAGAATGAATGGAC+GGG 0.644683 5_1:+25358578 None:intergenic
CGTTATTAGTGACAGGAGTG+AGG 0.644895 5_1:+25358496 None:intergenic
CGGTACAAGAGTCAGCGAGT+AGG 0.652069 5_1:+25358541 None:intergenic
TTTCGCGCATTCCACTGGCA+CGG 0.656401 5_1:+25358359 None:intergenic
ACCCCATCTGATGCTAACAA+TGG 0.658035 5_1:-25358393 Msa0725630:CDS
CTGATGGAGAGTTCTTGAGA+CGG 0.666946 5_1:+25358300 None:intergenic
GTGGTAAGCAGATGTCGCAA+GGG 0.667574 5_1:+25358228 None:intergenic
TCACGAACCTCAATCTCCTG+AGG 0.668607 5_1:-25358469 Msa0725630:CDS
TCAGCATTGAGGTCAAGTGG+CGG 0.682470 5_1:+25358327 None:intergenic
GTCAGCGAGTAGGTCAACCG+CGG 0.683086 5_1:+25358551 None:intergenic
GCCATTGTTAGCATCAGATG+GGG 0.683328 5_1:+25358392 None:intergenic
GCCAGATTTCTGGCTCAACA+CGG 0.683376 5_1:+25358727 None:intergenic
CCATCTGATGCTAACAATGG+CGG 0.690560 5_1:-25358390 Msa0725630:CDS
GATGTCGCAAGGGAGTCCCA+CGG 0.699974 5_1:+25358238 None:intergenic
CTGCTCCTAATCGTCCATGG+CGG 0.723801 5_1:-25357846 Msa0725630:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATTAACTTTCTTTTTT+AGG - chr5_1:25357687-25357706 Msa0725630:intron 10.0%
!!! TTAATTAACTTTCTTTTTTA+GGG - chr5_1:25357688-25357707 Msa0725630:intron 10.0%
!!! ATCATTTAAAATATATGATA+AGG + chr5_1:25358063-25358082 None:intergenic 10.0%
!!! TATCATATATTTTAAATGAT+TGG - chr5_1:25358063-25358082 Msa0725630:CDS 10.0%
!!! TTTAATTAACTTTCTTTTTT+AGG - chr5_1:25357687-25357706 Msa0725630:intron 10.0%
!!! TTAATTAACTTTCTTTTTTA+GGG - chr5_1:25357688-25357707 Msa0725630:intron 10.0%
!!! ATCATTTAAAATATATGATA+AGG + chr5_1:25358063-25358082 None:intergenic 10.0%
!!! TATCATATATTTTAAATGAT+TGG - chr5_1:25358063-25358082 Msa0725630:CDS 10.0%
!! AAAAAGAAAGTTAATTAAAC+AGG + chr5_1:25357686-25357705 None:intergenic 15.0%
!!! TATTTTTATCTCAATATCAT+AGG - chr5_1:25358100-25358119 Msa0725630:CDS 15.0%
!! AATGTTTAAACTCTATATTT+GGG + chr5_1:25358328-25358347 None:intergenic 15.0%
!! AAATGTTTAAACTCTATATT+TGG + chr5_1:25358329-25358348 None:intergenic 15.0%
!! AAAAAGAAAGTTAATTAAAC+AGG + chr5_1:25357686-25357705 None:intergenic 15.0%
!!! TATTTTTATCTCAATATCAT+AGG - chr5_1:25358100-25358119 Msa0725630:CDS 15.0%
!! AATGTTTAAACTCTATATTT+GGG + chr5_1:25358328-25358347 None:intergenic 15.0%
!! AAATGTTTAAACTCTATATT+TGG + chr5_1:25358329-25358348 None:intergenic 15.0%
!! TATTTCAACTTCATAAAGAA+TGG + chr5_1:25357790-25357809 None:intergenic 20.0%
!! ATTCTTTATGAAGTTGAAAT+AGG - chr5_1:25357789-25357808 Msa0725630:intron 20.0%
!! TTGTACAGTATACTTTATAT+AGG - chr5_1:25357847-25357866 Msa0725630:CDS 20.0%
!! AAACTTAAATACCATTTCTT+AGG + chr5_1:25357959-25357978 None:intergenic 20.0%
!!! ACATTAAGAGTATTTACAAT+AGG + chr5_1:25358001-25358020 None:intergenic 20.0%
!! AAATACTCTTAATGTTAAGT+AGG - chr5_1:25358006-25358025 Msa0725630:CDS 20.0%
!! TTTATGAATGATAAAAGCAA+TGG - chr5_1:25358452-25358471 Msa0725630:CDS 20.0%
!! TATTTCAACTTCATAAAGAA+TGG + chr5_1:25357790-25357809 None:intergenic 20.0%
!! ATTCTTTATGAAGTTGAAAT+AGG - chr5_1:25357789-25357808 Msa0725630:intron 20.0%
!! TTGTACAGTATACTTTATAT+AGG - chr5_1:25357847-25357866 Msa0725630:CDS 20.0%
!! AAACTTAAATACCATTTCTT+AGG + chr5_1:25357959-25357978 None:intergenic 20.0%
!!! ACATTAAGAGTATTTACAAT+AGG + chr5_1:25358001-25358020 None:intergenic 20.0%
!! AAATACTCTTAATGTTAAGT+AGG - chr5_1:25358006-25358025 Msa0725630:CDS 20.0%
!! TTTATGAATGATAAAAGCAA+TGG - chr5_1:25358452-25358471 Msa0725630:CDS 20.0%
!! TAAACACGAGAATATAGTTT+TGG + chr5_1:25356657-25356676 None:intergenic 25.0%
! TGAGTCTTTCATGAAAATTA+AGG + chr5_1:25357174-25357193 None:intergenic 25.0%
! GATAACAGAAAAATCGTTTA+CGG - chr5_1:25357250-25357269 Msa0725630:intron 25.0%
! ATGAATATTAAGTGGAATCT+TGG - chr5_1:25357380-25357399 Msa0725630:intron 25.0%
! AGTCATTGCAGTTAATTTAA+TGG + chr5_1:25357744-25357763 None:intergenic 25.0%
! TTCTTAGGAAATATCACTAT+TGG + chr5_1:25357944-25357963 None:intergenic 25.0%
! TAGTGATATTTCCTAAGAAA+TGG - chr5_1:25357945-25357964 Msa0725630:CDS 25.0%
! TCTCAATATCATAGGTTAAA+TGG - chr5_1:25358108-25358127 Msa0725630:CDS 25.0%
! CTCAATATCATAGGTTAAAT+GGG - chr5_1:25358109-25358128 Msa0725630:CDS 25.0%
!!! GATTTTCTAAGCTTTTTTGT+CGG + chr5_1:25358227-25358246 None:intergenic 25.0%
! TCAAAAAAGCCTCTATATTT+GGG + chr5_1:25358298-25358317 None:intergenic 25.0%
! TTCAAAAAAGCCTCTATATT+TGG + chr5_1:25358299-25358318 None:intergenic 25.0%
! ATTGTTCAACAATGTTTGAT+TGG + chr5_1:25358366-25358385 None:intergenic 25.0%
!! GCTTTTATCATTCATAAAGT+TGG + chr5_1:25358450-25358469 None:intergenic 25.0%
!!! TTTTCAAGAAGTAGATATCA+TGG - chr5_1:25358688-25358707 Msa0725630:CDS 25.0%
! TTTCAAGAAGTAGATATCAT+GGG - chr5_1:25358689-25358708 Msa0725630:CDS 25.0%
!! TAAACACGAGAATATAGTTT+TGG + chr5_1:25356657-25356676 None:intergenic 25.0%
! TGAGTCTTTCATGAAAATTA+AGG + chr5_1:25357174-25357193 None:intergenic 25.0%
! GATAACAGAAAAATCGTTTA+CGG - chr5_1:25357250-25357269 Msa0725630:intron 25.0%
! ATGAATATTAAGTGGAATCT+TGG - chr5_1:25357380-25357399 Msa0725630:intron 25.0%
! AGTCATTGCAGTTAATTTAA+TGG + chr5_1:25357744-25357763 None:intergenic 25.0%
! TTCTTAGGAAATATCACTAT+TGG + chr5_1:25357944-25357963 None:intergenic 25.0%
! TAGTGATATTTCCTAAGAAA+TGG - chr5_1:25357945-25357964 Msa0725630:CDS 25.0%
! TCTCAATATCATAGGTTAAA+TGG - chr5_1:25358108-25358127 Msa0725630:CDS 25.0%
! CTCAATATCATAGGTTAAAT+GGG - chr5_1:25358109-25358128 Msa0725630:CDS 25.0%
!!! GATTTTCTAAGCTTTTTTGT+CGG + chr5_1:25358227-25358246 None:intergenic 25.0%
! TCAAAAAAGCCTCTATATTT+GGG + chr5_1:25358298-25358317 None:intergenic 25.0%
! TTCAAAAAAGCCTCTATATT+TGG + chr5_1:25358299-25358318 None:intergenic 25.0%
! ATTGTTCAACAATGTTTGAT+TGG + chr5_1:25358366-25358385 None:intergenic 25.0%
!! GCTTTTATCATTCATAAAGT+TGG + chr5_1:25358450-25358469 None:intergenic 25.0%
!!! TTTTCAAGAAGTAGATATCA+TGG - chr5_1:25358688-25358707 Msa0725630:CDS 25.0%
! TTTCAAGAAGTAGATATCAT+GGG - chr5_1:25358689-25358708 Msa0725630:CDS 25.0%
TAGTTTGACGAATACTTCAT+CGG + chr5_1:25356811-25356830 None:intergenic 30.0%
! ATTTTCATGAAAGACTCAGA+CGG - chr5_1:25357176-25357195 Msa0725630:CDS 30.0%
AGGATGCAATGAATATTAAG+TGG - chr5_1:25357372-25357391 Msa0725630:intron 30.0%
AATATTAAGTGGAATCTTGG+AGG - chr5_1:25357383-25357402 Msa0725630:intron 30.0%
ATATTAAGTGGAATCTTGGA+GGG - chr5_1:25357384-25357403 Msa0725630:intron 30.0%
AAAGAGATGCTCAAAATTTG+AGG - chr5_1:25357442-25357461 Msa0725630:intron 30.0%
AAGAGATGCTCAAAATTTGA+GGG - chr5_1:25357443-25357462 Msa0725630:intron 30.0%
CTTGGTCTCATAATTAACTA+GGG + chr5_1:25357591-25357610 None:intergenic 30.0%
ACTTGGTCTCATAATTAACT+AGG + chr5_1:25357592-25357611 None:intergenic 30.0%
TTATGAGACCAAGTAAGTTA+CGG - chr5_1:25357598-25357617 Msa0725630:intron 30.0%
AAGATAAACCGTAACTTACT+TGG + chr5_1:25357609-25357628 None:intergenic 30.0%
CATAAACTGAGACAACTTAT+TGG + chr5_1:25357820-25357839 None:intergenic 30.0%
AAAGCTTAGAAAATCTGATC+AGG - chr5_1:25358232-25358251 Msa0725630:CDS 30.0%
!!! TATAGAGGCTTTTTTGAAGA+TGG - chr5_1:25358301-25358320 Msa0725630:CDS 30.0%
!! AAGTTGATGTGGAAAAAAGA+CGG + chr5_1:25358424-25358443 None:intergenic 30.0%
ATAAAAGCAATGGTTTCCTT+GGG - chr5_1:25358462-25358481 Msa0725630:CDS 30.0%
TTAGAAATATCATGGCTATG+AGG + chr5_1:25358563-25358582 None:intergenic 30.0%
CAAGAGAGTTAGAAATATCA+TGG + chr5_1:25358571-25358590 None:intergenic 30.0%
ATGGGAAATGATGATTGTAA+AGG - chr5_1:25358707-25358726 Msa0725630:CDS 30.0%
TTTGATGCCTTCAATTTCAT+TGG + chr5_1:25358735-25358754 None:intergenic 30.0%
TAGTTTGACGAATACTTCAT+CGG + chr5_1:25356811-25356830 None:intergenic 30.0%
! ATTTTCATGAAAGACTCAGA+CGG - chr5_1:25357176-25357195 Msa0725630:CDS 30.0%
AGGATGCAATGAATATTAAG+TGG - chr5_1:25357372-25357391 Msa0725630:intron 30.0%
AATATTAAGTGGAATCTTGG+AGG - chr5_1:25357383-25357402 Msa0725630:intron 30.0%
ATATTAAGTGGAATCTTGGA+GGG - chr5_1:25357384-25357403 Msa0725630:intron 30.0%
AAAGAGATGCTCAAAATTTG+AGG - chr5_1:25357442-25357461 Msa0725630:intron 30.0%
AAGAGATGCTCAAAATTTGA+GGG - chr5_1:25357443-25357462 Msa0725630:intron 30.0%
CTTGGTCTCATAATTAACTA+GGG + chr5_1:25357591-25357610 None:intergenic 30.0%
ACTTGGTCTCATAATTAACT+AGG + chr5_1:25357592-25357611 None:intergenic 30.0%
TTATGAGACCAAGTAAGTTA+CGG - chr5_1:25357598-25357617 Msa0725630:intron 30.0%
AAGATAAACCGTAACTTACT+TGG + chr5_1:25357609-25357628 None:intergenic 30.0%
CATAAACTGAGACAACTTAT+TGG + chr5_1:25357820-25357839 None:intergenic 30.0%
AAAGCTTAGAAAATCTGATC+AGG - chr5_1:25358232-25358251 Msa0725630:CDS 30.0%
!!! TATAGAGGCTTTTTTGAAGA+TGG - chr5_1:25358301-25358320 Msa0725630:CDS 30.0%
!! AAGTTGATGTGGAAAAAAGA+CGG + chr5_1:25358424-25358443 None:intergenic 30.0%
ATAAAAGCAATGGTTTCCTT+GGG - chr5_1:25358462-25358481 Msa0725630:CDS 30.0%
TTAGAAATATCATGGCTATG+AGG + chr5_1:25358563-25358582 None:intergenic 30.0%
CAAGAGAGTTAGAAATATCA+TGG + chr5_1:25358571-25358590 None:intergenic 30.0%
ATGGGAAATGATGATTGTAA+AGG - chr5_1:25358707-25358726 Msa0725630:CDS 30.0%
TTTGATGCCTTCAATTTCAT+TGG + chr5_1:25358735-25358754 None:intergenic 30.0%
GTGTTTACTACTTCCCATTA+GGG - chr5_1:25356670-25356689 Msa0725630:CDS 35.0%
GTTGACAAGAAGAAACTAGT+TGG - chr5_1:25357140-25357159 Msa0725630:CDS 35.0%
AACAGAAAAATCGTTTACGG+AGG - chr5_1:25357253-25357272 Msa0725630:intron 35.0%
TGAAGTTGTGTATTATCCGA+TGG - chr5_1:25357307-25357326 Msa0725630:intron 35.0%
AACTGAGACAACTTATTGGA+CGG + chr5_1:25357816-25357835 None:intergenic 35.0%
TGGATACAGTGCAAATATGT+CGG + chr5_1:25358195-25358214 None:intergenic 35.0%
!!! TTTTTTGTCGGTTGGAATTG+TGG + chr5_1:25358215-25358234 None:intergenic 35.0%
!!! TTCTAAGCTTTTTTGTCGGT+TGG + chr5_1:25358223-25358242 None:intergenic 35.0%
TGCTGCATTCAGTGATAAAA+AGG - chr5_1:25358259-25358278 Msa0725630:CDS 35.0%
GCTGCATTCAGTGATAAAAA+GGG - chr5_1:25358260-25358279 Msa0725630:CDS 35.0%
GTTCAACAATGTTTGATTGG+AGG + chr5_1:25358363-25358382 None:intergenic 35.0%
!! AAAGTTGGAAGAAGTTGATG+TGG + chr5_1:25358435-25358454 None:intergenic 35.0%
GATAAAAGCAATGGTTTCCT+TGG - chr5_1:25358461-25358480 Msa0725630:CDS 35.0%
TTTCCTTGGGAACAATTCTA+CGG - chr5_1:25358475-25358494 Msa0725630:CDS 35.0%
GATATAGGACCAAGATTGTT+TGG + chr5_1:25358527-25358546 None:intergenic 35.0%
CAATCTTGGTCCTATATCTT+TGG - chr5_1:25358529-25358548 Msa0725630:CDS 35.0%
CCAAATAGCATAAATGAACC+AGG + chr5_1:25358641-25358660 None:intergenic 35.0%
CCTGGTTCATTTATGCTATT+TGG - chr5_1:25358638-25358657 Msa0725630:CDS 35.0%
GGTAAGATCATAAAACCTGT+TGG - chr5_1:25358659-25358678 Msa0725630:CDS 35.0%
AAAGGCTCCAATGAAATTGA+AGG - chr5_1:25358725-25358744 Msa0725630:CDS 35.0%
GTGTTTACTACTTCCCATTA+GGG - chr5_1:25356670-25356689 Msa0725630:CDS 35.0%
GTTGACAAGAAGAAACTAGT+TGG - chr5_1:25357140-25357159 Msa0725630:CDS 35.0%
AACAGAAAAATCGTTTACGG+AGG - chr5_1:25357253-25357272 Msa0725630:intron 35.0%
TGAAGTTGTGTATTATCCGA+TGG - chr5_1:25357307-25357326 Msa0725630:intron 35.0%
AACTGAGACAACTTATTGGA+CGG + chr5_1:25357816-25357835 None:intergenic 35.0%
TGGATACAGTGCAAATATGT+CGG + chr5_1:25358195-25358214 None:intergenic 35.0%
!!! TTTTTTGTCGGTTGGAATTG+TGG + chr5_1:25358215-25358234 None:intergenic 35.0%
!!! TTCTAAGCTTTTTTGTCGGT+TGG + chr5_1:25358223-25358242 None:intergenic 35.0%
TGCTGCATTCAGTGATAAAA+AGG - chr5_1:25358259-25358278 Msa0725630:CDS 35.0%
GCTGCATTCAGTGATAAAAA+GGG - chr5_1:25358260-25358279 Msa0725630:CDS 35.0%
GTTCAACAATGTTTGATTGG+AGG + chr5_1:25358363-25358382 None:intergenic 35.0%
!! AAAGTTGGAAGAAGTTGATG+TGG + chr5_1:25358435-25358454 None:intergenic 35.0%
GATAAAAGCAATGGTTTCCT+TGG - chr5_1:25358461-25358480 Msa0725630:CDS 35.0%
TTTCCTTGGGAACAATTCTA+CGG - chr5_1:25358475-25358494 Msa0725630:CDS 35.0%
GATATAGGACCAAGATTGTT+TGG + chr5_1:25358527-25358546 None:intergenic 35.0%
CAATCTTGGTCCTATATCTT+TGG - chr5_1:25358529-25358548 Msa0725630:CDS 35.0%
CCAAATAGCATAAATGAACC+AGG + chr5_1:25358641-25358660 None:intergenic 35.0%
CCTGGTTCATTTATGCTATT+TGG - chr5_1:25358638-25358657 Msa0725630:CDS 35.0%
GGTAAGATCATAAAACCTGT+TGG - chr5_1:25358659-25358678 Msa0725630:CDS 35.0%
AAAGGCTCCAATGAAATTGA+AGG - chr5_1:25358725-25358744 Msa0725630:CDS 35.0%
CGTGTTTACTACTTCCCATT+AGG - chr5_1:25356669-25356688 Msa0725630:CDS 40.0%
! TGTGTTCTAAGTGCCCTAAT+GGG + chr5_1:25356686-25356705 None:intergenic 40.0%
! ATGTGTTCTAAGTGCCCTAA+TGG + chr5_1:25356687-25356706 None:intergenic 40.0%
CTCACTCCTGTCACTAATAA+CGG - chr5_1:25356834-25356853 Msa0725630:CDS 40.0%
TGAACACCGTTATTAGTGAC+AGG + chr5_1:25356843-25356862 None:intergenic 40.0%
! AGACCAAGATGATGGCAAAA+AGG - chr5_1:25356890-25356909 Msa0725630:CDS 40.0%
! CAACCTTTTTGCCATCATCT+TGG + chr5_1:25356896-25356915 None:intergenic 40.0%
ATTAATTTCGCGCATTCCAC+TGG + chr5_1:25356978-25356997 None:intergenic 40.0%
!! TTTTCAGCATTGAGGTCAAG+TGG + chr5_1:25357008-25357027 None:intergenic 40.0%
CTCTCCATCAGTGATATTCA+CGG - chr5_1:25357041-25357060 Msa0725630:CDS 40.0%
TTTGCCGTGAATATCACTGA+TGG + chr5_1:25357048-25357067 None:intergenic 40.0%
GTGATATTCACGGCAAAGTT+TGG - chr5_1:25357051-25357070 Msa0725630:CDS 40.0%
GACAAGAAGAAACTAGTTGG+CGG - chr5_1:25357143-25357162 Msa0725630:CDS 40.0%
TCAGACGGAAATATCTCAGT+CGG - chr5_1:25357191-25357210 Msa0725630:CDS 40.0%
! AAAATCGTTTACGGAGGCAT+TGG - chr5_1:25357259-25357278 Msa0725630:intron 40.0%
GTGTATTATCCGATGGCTAA+AGG - chr5_1:25357314-25357333 Msa0725630:intron 40.0%
ATTATCCGATGGCTAAAGGT+TGG - chr5_1:25357318-25357337 Msa0725630:intron 40.0%
TTACACCAACCTTTAGCCAT+CGG + chr5_1:25357326-25357345 None:intergenic 40.0%
TGTTGTTGATGCCAAAGTTG+TGG - chr5_1:25357349-25357368 Msa0725630:intron 40.0%
!! CACTATTGGAGCAAGTTGAT+AGG + chr5_1:25357930-25357949 None:intergenic 40.0%
GAAATAGTTTCAACCTCCCA+AGG + chr5_1:25358173-25358192 None:intergenic 40.0%
TCAAGTGAGCCAAACAATCT+TGG - chr5_1:25358515-25358534 Msa0725630:CDS 40.0%
GGTGACAAGTCCAAAGATAT+AGG + chr5_1:25358542-25358561 None:intergenic 40.0%
TCTCTTGTGACAAAGTTTGC+CGG - chr5_1:25358584-25358603 Msa0725630:CDS 40.0%
AACACCAAGAAAGTTAGTCC+TGG - chr5_1:25358620-25358639 Msa0725630:CDS 40.0%
TGAACCAGGACTAACTTTCT+TGG + chr5_1:25358627-25358646 None:intergenic 40.0%
TCTTGAAAATCGCTTCCAAC+AGG + chr5_1:25358677-25358696 None:intergenic 40.0%
CGTGTTTACTACTTCCCATT+AGG - chr5_1:25356669-25356688 Msa0725630:CDS 40.0%
! TGTGTTCTAAGTGCCCTAAT+GGG + chr5_1:25356686-25356705 None:intergenic 40.0%
! ATGTGTTCTAAGTGCCCTAA+TGG + chr5_1:25356687-25356706 None:intergenic 40.0%
CTCACTCCTGTCACTAATAA+CGG - chr5_1:25356834-25356853 Msa0725630:CDS 40.0%
TGAACACCGTTATTAGTGAC+AGG + chr5_1:25356843-25356862 None:intergenic 40.0%
! AGACCAAGATGATGGCAAAA+AGG - chr5_1:25356890-25356909 Msa0725630:CDS 40.0%
! CAACCTTTTTGCCATCATCT+TGG + chr5_1:25356896-25356915 None:intergenic 40.0%
ATTAATTTCGCGCATTCCAC+TGG + chr5_1:25356978-25356997 None:intergenic 40.0%
!! TTTTCAGCATTGAGGTCAAG+TGG + chr5_1:25357008-25357027 None:intergenic 40.0%
CTCTCCATCAGTGATATTCA+CGG - chr5_1:25357041-25357060 Msa0725630:CDS 40.0%
TTTGCCGTGAATATCACTGA+TGG + chr5_1:25357048-25357067 None:intergenic 40.0%
GTGATATTCACGGCAAAGTT+TGG - chr5_1:25357051-25357070 Msa0725630:CDS 40.0%
GACAAGAAGAAACTAGTTGG+CGG - chr5_1:25357143-25357162 Msa0725630:CDS 40.0%
TCAGACGGAAATATCTCAGT+CGG - chr5_1:25357191-25357210 Msa0725630:CDS 40.0%
! AAAATCGTTTACGGAGGCAT+TGG - chr5_1:25357259-25357278 Msa0725630:intron 40.0%
GTGTATTATCCGATGGCTAA+AGG - chr5_1:25357314-25357333 Msa0725630:intron 40.0%
ATTATCCGATGGCTAAAGGT+TGG - chr5_1:25357318-25357337 Msa0725630:intron 40.0%
TTACACCAACCTTTAGCCAT+CGG + chr5_1:25357326-25357345 None:intergenic 40.0%
TGTTGTTGATGCCAAAGTTG+TGG - chr5_1:25357349-25357368 Msa0725630:intron 40.0%
!! CACTATTGGAGCAAGTTGAT+AGG + chr5_1:25357930-25357949 None:intergenic 40.0%
GAAATAGTTTCAACCTCCCA+AGG + chr5_1:25358173-25358192 None:intergenic 40.0%
TCAAGTGAGCCAAACAATCT+TGG - chr5_1:25358515-25358534 Msa0725630:CDS 40.0%
GGTGACAAGTCCAAAGATAT+AGG + chr5_1:25358542-25358561 None:intergenic 40.0%
TCTCTTGTGACAAAGTTTGC+CGG - chr5_1:25358584-25358603 Msa0725630:CDS 40.0%
AACACCAAGAAAGTTAGTCC+TGG - chr5_1:25358620-25358639 Msa0725630:CDS 40.0%
TGAACCAGGACTAACTTTCT+TGG + chr5_1:25358627-25358646 None:intergenic 40.0%
TCTTGAAAATCGCTTCCAAC+AGG + chr5_1:25358677-25358696 None:intergenic 40.0%
! CAAGAAGGGAGAGTGTGTTA+GGG + chr5_1:25356725-25356744 None:intergenic 45.0%
GGTGCATAGAATGAATGGAC+GGG + chr5_1:25356754-25356773 None:intergenic 45.0%
CGGTGCATAGAATGAATGGA+CGG + chr5_1:25356755-25356774 None:intergenic 45.0%
GAGACGGTGCATAGAATGAA+TGG + chr5_1:25356759-25356778 None:intergenic 45.0%
CGTTATTAGTGACAGGAGTG+AGG + chr5_1:25356836-25356855 None:intergenic 45.0%
GTTCGTGAAGACCAAGATGA+TGG - chr5_1:25356882-25356901 Msa0725630:CDS 45.0%
ACCCCATCTGATGCTAACAA+TGG - chr5_1:25356936-25356955 Msa0725630:CDS 45.0%
GCCATTGTTAGCATCAGATG+GGG + chr5_1:25356940-25356959 None:intergenic 45.0%
CGCCATTGTTAGCATCAGAT+GGG + chr5_1:25356941-25356960 None:intergenic 45.0%
CCATCTGATGCTAACAATGG+CGG - chr5_1:25356939-25356958 Msa0725630:CDS 45.0%
CTGATGGAGAGTTCTTGAGA+CGG + chr5_1:25357032-25357051 None:intergenic 45.0%
! AATTTTCGCCATGTCTACCG+TGG - chr5_1:25357074-25357093 Msa0725630:CDS 45.0%
! ATTTTCGCCATGTCTACCGT+GGG - chr5_1:25357075-25357094 Msa0725630:CDS 45.0%
GACATCTGCTTACCACTGAT+TGG - chr5_1:25357108-25357127 Msa0725630:CDS 45.0%
AACGAACTCACTCCAATCAG+TGG + chr5_1:25357123-25357142 None:intergenic 45.0%
CAGCACCAAACTTTGTCTGT+CGG + chr5_1:25357223-25357242 None:intergenic 45.0%
!! GTTTACGGAGGCATTGGAAT+TGG - chr5_1:25357265-25357284 Msa0725630:intron 45.0%
! GGAATTGGCGGATAAGAACA+TGG - chr5_1:25357280-25357299 Msa0725630:intron 45.0%
TGTTGATGCCAAAGTTGTGG+AGG - chr5_1:25357352-25357371 Msa0725630:intron 45.0%
CATTGCATCCTCCACAACTT+TGG + chr5_1:25357363-25357382 None:intergenic 45.0%
TATCTGCTCCTAATCGTCCA+TGG - chr5_1:25357480-25357499 Msa0725630:intron 45.0%
CTCCTTCGTCCCAAATATAG+AGG - chr5_1:25358286-25358305 Msa0725630:CDS 45.0%
! AGCCTCTATATTTGGGACGA+AGG + chr5_1:25358291-25358310 None:intergenic 45.0%
TGCTCTTCTTGATTGCATGC+AGG - chr5_1:25358388-25358407 Msa0725630:CDS 45.0%
GCTCTTCTTGATTGCATGCA+GGG - chr5_1:25358389-25358408 Msa0725630:CDS 45.0%
ATGTGGAAAAAAGACGGTGC+CGG + chr5_1:25358418-25358437 None:intergenic 45.0%
! CAAGAAGGGAGAGTGTGTTA+GGG + chr5_1:25356725-25356744 None:intergenic 45.0%
GGTGCATAGAATGAATGGAC+GGG + chr5_1:25356754-25356773 None:intergenic 45.0%
CGGTGCATAGAATGAATGGA+CGG + chr5_1:25356755-25356774 None:intergenic 45.0%
GAGACGGTGCATAGAATGAA+TGG + chr5_1:25356759-25356778 None:intergenic 45.0%
CGTTATTAGTGACAGGAGTG+AGG + chr5_1:25356836-25356855 None:intergenic 45.0%
GTTCGTGAAGACCAAGATGA+TGG - chr5_1:25356882-25356901 Msa0725630:CDS 45.0%
ACCCCATCTGATGCTAACAA+TGG - chr5_1:25356936-25356955 Msa0725630:CDS 45.0%
GCCATTGTTAGCATCAGATG+GGG + chr5_1:25356940-25356959 None:intergenic 45.0%
CGCCATTGTTAGCATCAGAT+GGG + chr5_1:25356941-25356960 None:intergenic 45.0%
CCATCTGATGCTAACAATGG+CGG - chr5_1:25356939-25356958 Msa0725630:CDS 45.0%
CTGATGGAGAGTTCTTGAGA+CGG + chr5_1:25357032-25357051 None:intergenic 45.0%
! AATTTTCGCCATGTCTACCG+TGG - chr5_1:25357074-25357093 Msa0725630:CDS 45.0%
! ATTTTCGCCATGTCTACCGT+GGG - chr5_1:25357075-25357094 Msa0725630:CDS 45.0%
GACATCTGCTTACCACTGAT+TGG - chr5_1:25357108-25357127 Msa0725630:CDS 45.0%
AACGAACTCACTCCAATCAG+TGG + chr5_1:25357123-25357142 None:intergenic 45.0%
CAGCACCAAACTTTGTCTGT+CGG + chr5_1:25357223-25357242 None:intergenic 45.0%
!! GTTTACGGAGGCATTGGAAT+TGG - chr5_1:25357265-25357284 Msa0725630:intron 45.0%
! GGAATTGGCGGATAAGAACA+TGG - chr5_1:25357280-25357299 Msa0725630:intron 45.0%
TGTTGATGCCAAAGTTGTGG+AGG - chr5_1:25357352-25357371 Msa0725630:intron 45.0%
CATTGCATCCTCCACAACTT+TGG + chr5_1:25357363-25357382 None:intergenic 45.0%
TATCTGCTCCTAATCGTCCA+TGG - chr5_1:25357480-25357499 Msa0725630:intron 45.0%
CTCCTTCGTCCCAAATATAG+AGG - chr5_1:25358286-25358305 Msa0725630:CDS 45.0%
! AGCCTCTATATTTGGGACGA+AGG + chr5_1:25358291-25358310 None:intergenic 45.0%
TGCTCTTCTTGATTGCATGC+AGG - chr5_1:25358388-25358407 Msa0725630:CDS 45.0%
GCTCTTCTTGATTGCATGCA+GGG - chr5_1:25358389-25358408 Msa0725630:CDS 45.0%
ATGTGGAAAAAAGACGGTGC+CGG + chr5_1:25358418-25358437 None:intergenic 45.0%
!! ATTTCTGGCTCAACACGGCT+GGG + chr5_1:25356600-25356619 None:intergenic 50.0%
!! GCCAGATTTCTGGCTCAACA+CGG + chr5_1:25356605-25356624 None:intergenic 50.0%
AGTGTGTTAGGGTTTGGGGA+TGG + chr5_1:25356714-25356733 None:intergenic 50.0%
! GGAGAGTGTGTTAGGGTTTG+GGG + chr5_1:25356718-25356737 None:intergenic 50.0%
! GGGAGAGTGTGTTAGGGTTT+GGG + chr5_1:25356719-25356738 None:intergenic 50.0%
! AGGGAGAGTGTGTTAGGGTT+TGG + chr5_1:25356720-25356739 None:intergenic 50.0%
! CCAAGAAGGGAGAGTGTGTT+AGG + chr5_1:25356726-25356745 None:intergenic 50.0%
CCTAACACACTCTCCCTTCT+TGG - chr5_1:25356723-25356742 Msa0725630:CDS 50.0%
TCACGAACCTCAATCTCCTG+AGG - chr5_1:25356860-25356879 Msa0725630:CDS 50.0%
TCACGAACCTCAGGAGATTG+AGG + chr5_1:25356870-25356889 None:intergenic 50.0%
! TCTTGGTCTTCACGAACCTC+AGG + chr5_1:25356879-25356898 None:intergenic 50.0%
TGGGGTGAGAGTCTTGGTAT+AGG + chr5_1:25356922-25356941 None:intergenic 50.0%
ATCAGATGGGGTGAGAGTCT+TGG + chr5_1:25356928-25356947 None:intergenic 50.0%
CCGCCATTGTTAGCATCAGA+TGG + chr5_1:25356942-25356961 None:intergenic 50.0%
! TCAGCATTGAGGTCAAGTGG+CGG + chr5_1:25357005-25357024 None:intergenic 50.0%
! GAGACGGCTTTTCAGCATTG+AGG + chr5_1:25357016-25357035 None:intergenic 50.0%
GTGGTAAGCAGATGTCGCAA+GGG + chr5_1:25357104-25357123 None:intergenic 50.0%
AGTGGTAAGCAGATGTCGCA+AGG + chr5_1:25357105-25357124 None:intergenic 50.0%
GTTCGCCGACAGACAAAGTT+TGG - chr5_1:25357215-25357234 Msa0725630:intron 50.0%
!! TACGGAGGCATTGGAATTGG+CGG - chr5_1:25357268-25357287 Msa0725630:intron 50.0%
TATACCTCAAGCATCCGCCA+TGG + chr5_1:25357500-25357519 None:intergenic 50.0%
! TCTGGAACGTGCGAGACTTT+AGG + chr5_1:25358140-25358159 None:intergenic 50.0%
TTCCAGAGAGAGTCAGTCCT+TGG - chr5_1:25358153-25358172 Msa0725630:CDS 50.0%
TCCAGAGAGAGTCAGTCCTT+GGG - chr5_1:25358154-25358173 Msa0725630:CDS 50.0%
TGTGGAAAAAAGACGGTGCC+GGG + chr5_1:25358417-25358436 None:intergenic 50.0%
GTGCCGTAGAATTGTTCCCA+AGG + chr5_1:25358481-25358500 None:intergenic 50.0%
!! GGTGTTGCTGTTGTCAGTAC+CGG + chr5_1:25358606-25358625 None:intergenic 50.0%
!! ATTTCTGGCTCAACACGGCT+GGG + chr5_1:25356600-25356619 None:intergenic 50.0%
!! GCCAGATTTCTGGCTCAACA+CGG + chr5_1:25356605-25356624 None:intergenic 50.0%
AGTGTGTTAGGGTTTGGGGA+TGG + chr5_1:25356714-25356733 None:intergenic 50.0%
! GGAGAGTGTGTTAGGGTTTG+GGG + chr5_1:25356718-25356737 None:intergenic 50.0%
! GGGAGAGTGTGTTAGGGTTT+GGG + chr5_1:25356719-25356738 None:intergenic 50.0%
! AGGGAGAGTGTGTTAGGGTT+TGG + chr5_1:25356720-25356739 None:intergenic 50.0%
! CCAAGAAGGGAGAGTGTGTT+AGG + chr5_1:25356726-25356745 None:intergenic 50.0%
CCTAACACACTCTCCCTTCT+TGG - chr5_1:25356723-25356742 Msa0725630:CDS 50.0%
TCACGAACCTCAATCTCCTG+AGG - chr5_1:25356860-25356879 Msa0725630:CDS 50.0%
TCACGAACCTCAGGAGATTG+AGG + chr5_1:25356870-25356889 None:intergenic 50.0%
! TCTTGGTCTTCACGAACCTC+AGG + chr5_1:25356879-25356898 None:intergenic 50.0%
TGGGGTGAGAGTCTTGGTAT+AGG + chr5_1:25356922-25356941 None:intergenic 50.0%
ATCAGATGGGGTGAGAGTCT+TGG + chr5_1:25356928-25356947 None:intergenic 50.0%
CCGCCATTGTTAGCATCAGA+TGG + chr5_1:25356942-25356961 None:intergenic 50.0%
! TCAGCATTGAGGTCAAGTGG+CGG + chr5_1:25357005-25357024 None:intergenic 50.0%
! GAGACGGCTTTTCAGCATTG+AGG + chr5_1:25357016-25357035 None:intergenic 50.0%
GTGGTAAGCAGATGTCGCAA+GGG + chr5_1:25357104-25357123 None:intergenic 50.0%
AGTGGTAAGCAGATGTCGCA+AGG + chr5_1:25357105-25357124 None:intergenic 50.0%
GTTCGCCGACAGACAAAGTT+TGG - chr5_1:25357215-25357234 Msa0725630:intron 50.0%
!! TACGGAGGCATTGGAATTGG+CGG - chr5_1:25357268-25357287 Msa0725630:intron 50.0%
TATACCTCAAGCATCCGCCA+TGG + chr5_1:25357500-25357519 None:intergenic 50.0%
! TCTGGAACGTGCGAGACTTT+AGG + chr5_1:25358140-25358159 None:intergenic 50.0%
TTCCAGAGAGAGTCAGTCCT+TGG - chr5_1:25358153-25358172 Msa0725630:CDS 50.0%
TCCAGAGAGAGTCAGTCCTT+GGG - chr5_1:25358154-25358173 Msa0725630:CDS 50.0%
TGTGGAAAAAAGACGGTGCC+GGG + chr5_1:25358417-25358436 None:intergenic 50.0%
GTGCCGTAGAATTGTTCCCA+AGG + chr5_1:25358481-25358500 None:intergenic 50.0%
!! GGTGTTGCTGTTGTCAGTAC+CGG + chr5_1:25358606-25358625 None:intergenic 50.0%
!! GATTTCTGGCTCAACACGGC+TGG + chr5_1:25356601-25356620 None:intergenic 55.0%
GCCGTGTTGAGCCAGAAATC+TGG - chr5_1:25356601-25356620 Msa0725630:CDS 55.0%
GCACAGCACTGCCAGATTTC+TGG + chr5_1:25356615-25356634 None:intergenic 55.0%
GAAATCTGGCAGTGCTGTGC+AGG - chr5_1:25356615-25356634 Msa0725630:CDS 55.0%
TGGACGGGAACTGCCAAGAA+GGG + chr5_1:25356739-25356758 None:intergenic 55.0%
ATGGACGGGAACTGCCAAGA+AGG + chr5_1:25356740-25356759 None:intergenic 55.0%
CATTCTATGCACCGTCTCCG+CGG - chr5_1:25356761-25356780 Msa0725630:CDS 55.0%
CGGTACAAGAGTCAGCGAGT+AGG + chr5_1:25356791-25356810 None:intergenic 55.0%
TTTCGCGCATTCCACTGGCA+CGG + chr5_1:25356973-25356992 None:intergenic 55.0%
! CAGCATTGAGGTCAAGTGGC+GGG + chr5_1:25357004-25357023 None:intergenic 55.0%
CTGCTCCTAATCGTCCATGG+CGG - chr5_1:25357483-25357502 Msa0725630:intron 55.0%
AGCATCCGCCATGGACGATT+AGG + chr5_1:25357491-25357510 None:intergenic 55.0%
! TCCCAAGGACTGACTCTCTC+TGG + chr5_1:25358158-25358177 None:intergenic 55.0%
AGAGAGAGTCAGTCCTTGGG+AGG - chr5_1:25358157-25358176 Msa0725630:CDS 55.0%
TTGATTGCATGCAGGGAGCC+CGG - chr5_1:25358396-25358415 Msa0725630:CDS 55.0%
!! GATTTCTGGCTCAACACGGC+TGG + chr5_1:25356601-25356620 None:intergenic 55.0%
GCCGTGTTGAGCCAGAAATC+TGG - chr5_1:25356601-25356620 Msa0725630:CDS 55.0%
GCACAGCACTGCCAGATTTC+TGG + chr5_1:25356615-25356634 None:intergenic 55.0%
GAAATCTGGCAGTGCTGTGC+AGG - chr5_1:25356615-25356634 Msa0725630:CDS 55.0%
TGGACGGGAACTGCCAAGAA+GGG + chr5_1:25356739-25356758 None:intergenic 55.0%
ATGGACGGGAACTGCCAAGA+AGG + chr5_1:25356740-25356759 None:intergenic 55.0%
CATTCTATGCACCGTCTCCG+CGG - chr5_1:25356761-25356780 Msa0725630:CDS 55.0%
CGGTACAAGAGTCAGCGAGT+AGG + chr5_1:25356791-25356810 None:intergenic 55.0%
TTTCGCGCATTCCACTGGCA+CGG + chr5_1:25356973-25356992 None:intergenic 55.0%
! CAGCATTGAGGTCAAGTGGC+GGG + chr5_1:25357004-25357023 None:intergenic 55.0%
CTGCTCCTAATCGTCCATGG+CGG - chr5_1:25357483-25357502 Msa0725630:intron 55.0%
AGCATCCGCCATGGACGATT+AGG + chr5_1:25357491-25357510 None:intergenic 55.0%
! TCCCAAGGACTGACTCTCTC+TGG + chr5_1:25358158-25358177 None:intergenic 55.0%
AGAGAGAGTCAGTCCTTGGG+AGG - chr5_1:25358157-25358176 Msa0725630:CDS 55.0%
TTGATTGCATGCAGGGAGCC+CGG - chr5_1:25358396-25358415 Msa0725630:CDS 55.0%
GCAGTGCTGTGCAGGCAGTT+CGG - chr5_1:25356623-25356642 Msa0725630:CDS 60.0%
GAGTAGGTCAACCGCGGAGA+CGG + chr5_1:25356775-25356794 None:intergenic 60.0%
GTCAGCGAGTAGGTCAACCG+CGG + chr5_1:25356781-25356800 None:intergenic 60.0%
AGGGAGTCCCACGGTAGACA+TGG + chr5_1:25357085-25357104 None:intergenic 60.0%
GATGTCGCAAGGGAGTCCCA+CGG + chr5_1:25357094-25357113 None:intergenic 60.0%
!! TCGTCCATGGCGGATGCTTG+AGG - chr5_1:25357493-25357512 Msa0725630:intron 60.0%
GCAGTGCTGTGCAGGCAGTT+CGG - chr5_1:25356623-25356642 Msa0725630:CDS 60.0%
GAGTAGGTCAACCGCGGAGA+CGG + chr5_1:25356775-25356794 None:intergenic 60.0%
GTCAGCGAGTAGGTCAACCG+CGG + chr5_1:25356781-25356800 None:intergenic 60.0%
AGGGAGTCCCACGGTAGACA+TGG + chr5_1:25357085-25357104 None:intergenic 60.0%
GATGTCGCAAGGGAGTCCCA+CGG + chr5_1:25357094-25357113 None:intergenic 60.0%
!! TCGTCCATGGCGGATGCTTG+AGG - chr5_1:25357493-25357512 Msa0725630:intron 60.0%
CGGTGCATTCTCCGTGCCAG+TGG - chr5_1:25356959-25356978 Msa0725630:CDS 65.0%
CGGTGCATTCTCCGTGCCAG+TGG - chr5_1:25356959-25356978 Msa0725630:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 25356582 25358769 25356582 ID=Msa0725630;Name=Msa0725630
chr5_1 mRNA 25356582 25358769 25356582 ID=Msa0725630-mRNA-1;Parent=Msa0725630;Name=Msa0725630-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|526
chr5_1 exon 25357837 25358769 25357837 ID=Msa0725630-mRNA-1:exon:9176;Parent=Msa0725630-mRNA-1
chr5_1 exon 25356582 25357229 25356582 ID=Msa0725630-mRNA-1:exon:9175;Parent=Msa0725630-mRNA-1
chr5_1 CDS 25357837 25358769 25357837 ID=Msa0725630-mRNA-1:cds;Parent=Msa0725630-mRNA-1
chr5_1 CDS 25356582 25357229 25356582 ID=Msa0725630-mRNA-1:cds;Parent=Msa0725630-mRNA-1
Gene Sequence

>Msa0725630

ATGTCTTCTCAGCAACCCAGCCGTGTTGAGCCAGAAATCTGGCAGTGCTGTGCAGGCAGTTCGGTTAAAATTCCAAAACTATATTCTCGTGTTTACTACTTCCCATTAGGGCACTTAGAACACATATGTCCATCCCCAAACCCTAACACACTCTCCCTTCTTGGCAGTTCCCGTCCATTCATTCTATGCACCGTCTCCGCGGTTGACCTACTCGCTGACTCTTGTACCGATGAAGTATTCGTCAAACTACTCCTCACTCCTGTCACTAATAACGGTGTTCACGAACCTCAATCTCCTGAGGTTCGTGAAGACCAAGATGATGGCAAAAAGGTTGTTTCCTATACCAAGACTCTCACCCCATCTGATGCTAACAATGGCGGTGCATTCTCCGTGCCAGTGGAATGCGCGAAATTAATCTTCCCGCCACTTGACCTCAATGCTGAAAAGCCGTCTCAAGAACTCTCCATCAGTGATATTCACGGCAAAGTTTGGAATTTTCGCCATGTCTACCGTGGGACTCCCTTGCGACATCTGCTTACCACTGATTGGAGTGAGTTCGTTGACAAGAAGAAACTAGTTGGCGGCGATTCCTTAATTTTCATGAAAGACTCAGACGGAAATATCTCAGTCGGAGTTCGCCGACAGACAAAGTTTGGTGCTGCTGCTAAGATAACAGAAAAATCGTTTACGGAGGCATTGGAATTGGCGGATAAGAACATGGCTTTTGAAGTTGTGTATTATCCGATGGCTAAAGGTTGGTGTAATTTTGTTGTTGATGCCAAAGTTGTGGAGGATGCAATGAATATTAAGTGGAATCTTGGAGGGAGAATTGAACTTTCTTTGAAGAATTTCGATTCTTCAAAGAGATGCTCAAAATTTGAGGGAACAATATCTGCTCTATCTGCTCCTAATCGTCCATGGCGGATGCTTGAGGTTAAATGGGATGAACCTAAAGTCTCGCACGTTCCAGAGAGAGTCAGTCCTTGGGAGGTTGAAACTATTTCCGACATATTTGCACTGTATCCACAATTCCAACCGACAAAAAAGCTTAGAAAATCTGATCAGGATTCTGCTGCATTCAGTGATAAAAAGGGAGACTCCTTCGTCCCAAATATAGAGGCTTTTTTGAAGATGGTCCCAAATATAGAGTTTAAACATTTCGATATGACATCCTCCAATCAAACATTGTTGAACAATGATGCTCTTCTTGATTGCATGCAGGGAGCCCGGCACCGTCTTTTTTCCACATCAACTTCTTCCAACTTTATGAATGATAAAAGCAATGGTTTCCTTGGGAACAATTCTACGGCACTGTTGAGTAATGTGTCAAGTGAGCCAAACAATCTTGGTCCTATATCTTTGGACTTGTCACCTCATAGCCATGATATTTCTAACTCTCTTGTGACAAAGTTTGCCGGTACTGACAACAGCAACACCAAGAAAGTTAGTCCTGGTTCATTTATGCTATTTGGTAAGATCATAAAACCTGTTGGAAGCGATTTTCAAGAAGTAGATATCATGGGAAATGATGATTGTAAAGGCTCCAATGAAATTGAAGGCATCAAACTTGCACAAAAATAG
Protein sequence

>Msa0725630

MSSQQPSRVEPEIWQCCAGSSVKIPKLYSRVYYFPLGHLEHICPSPNPNTLSLLGSSRPFILCTVSAVDLLADSCTDEVFVKLLLTPVTNNGVHEPQSPEVREDQDDGKKVVSYTKTLTPSDANNGGAFSVPVECAKLIFPPLDLNAEKPSQELSISDIHGKVWNFRHVYRGTPLRHLLTTDWSEFVDKKKLVGGDSLIFMKDSDGNISVGVRRQTKFGAAAKITEKSFTEALELADKNMAFEVVYYPMAKGWCNFVVDAKVVEDAMNIKWNLGGRIELSLKNFDSSKRCSKFEGTISALSAPNRPWRMLEVKWDEPKVSHVPERVSPWEVETISDIFALYPQFQPTKKLRKSDQDSAAFSDKKGDSFVPNIEAFLKMVPNIEFKHFDMTSSNQTLLNNDALLDCMQGARHRLFSTSTSSNFMNDKSNGFLGNNSTALLSNVSSEPNNLGPISLDLSPHSHDISNSLVTKFAGTDNSNTKKVSPGSFMLFGKIIKPVGSDFQEVDIMGNDDCKGSNEIEGIKLAQK*