AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0725910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0725910 MtrunA17_Chr4g0060841 49.036 363 165 7 1 352 1 354 7.42e-110 325
Msa0725910 MtrunA17_Chr5g0417661 100.000 61 0 0 1 61 56 116 3.78e-37 130
Msa0725910 MtrunA17_Chr3g0134451 33.880 183 102 5 1 173 1 174 7.40e-27 108
Msa0725910 MtrunA17_Chr5g0417671 96.078 51 2 0 59 109 26 76 3.04e-26 100
Msa0725910 MtrunA17_Chr5g0425191 65.574 61 21 0 1 61 1 61 6.18e-22 89.4
Msa0725910 MtrunA17_Chr4g0044071 65.574 61 21 0 1 61 1 61 1.74e-20 85.9
Msa0725910 MtrunA17_Chr3g0125241 58.491 53 22 0 1 53 26 78 4.15e-15 73.6
Msa0725910 MtrunA17_Chr3g0077501 56.604 53 23 0 1 53 1 53 5.42e-15 73.6
Msa0725910 MtrunA17_Chr3g0097101 56.604 53 23 0 1 53 16 68 5.49e-15 73.9
Msa0725910 MtrunA17_Chr2g0284911 44.737 76 40 1 1 76 16 89 5.58e-15 74.3
Msa0725910 MtrunA17_Chr5g0427401 30.769 169 89 3 1 169 1 141 5.66e-15 73.6
Msa0725910 MtrunA17_Chr8g0351041 30.000 180 101 3 1 180 1 155 6.63e-15 71.6
Msa0725910 MtrunA17_Chr5g0420091 53.333 60 27 1 1 59 1 60 8.80e-15 72.8
Msa0725910 MtrunA17_Chr7g0220111 32.240 183 92 7 1 180 1 154 1.04e-14 73.2
Msa0725910 MtrunA17_Chr1g0175611 51.852 54 26 0 1 54 1 54 1.46e-14 70.9
Msa0725910 MtrunA17_Chr8g0377561 54.717 53 24 0 1 53 1 53 1.56e-14 68.9
Msa0725910 MtrunA17_Chr1g0173161 52.830 53 25 0 1 53 1 53 1.61e-14 68.6
Msa0725910 MtrunA17_Chr8g0380021 54.717 53 24 0 1 53 17 69 1.63e-14 72.4
Msa0725910 MtrunA17_Chr1g0159661 34.615 104 68 0 1 104 1 104 1.76e-14 71.2
Msa0725910 MtrunA17_Chr4g0061671 54.717 53 24 0 1 53 1 53 2.22e-14 70.9
Msa0725910 MtrunA17_Chr8g0351031 29.444 180 102 3 1 180 1 155 2.31e-14 71.6
Msa0725910 MtrunA17_Chr4g0020221 54.717 53 24 0 1 53 1 53 2.35e-14 72.4
Msa0725910 MtrunA17_Chr7g0246381 54.545 55 25 0 1 55 1 55 2.51e-14 67.4
Msa0725910 MtrunA17_Chr7g0220121 28.729 181 98 5 1 179 1 152 2.56e-14 72.0
Msa0725910 MtrunA17_Chr3g0142191 56.604 53 23 0 1 53 1 53 2.94e-14 71.6
Msa0725910 MtrunA17_Chr7g0246431 54.717 53 24 0 1 53 1 53 3.25e-14 70.9
Msa0725910 MtrunA17_Chr2g0279821 54.717 53 24 0 1 53 1 53 5.80e-14 70.9
Msa0725910 MtrunA17_Chr3g0122251 31.148 183 98 6 1 180 1 158 5.88e-14 70.9
Msa0725910 MtrunA17_Chr5g0412461 52.830 53 25 0 1 53 1 53 7.11e-14 70.5
Msa0725910 MtrunA17_Chr5g0412271 52.830 53 25 0 1 53 1 53 9.10e-14 70.1
Msa0725910 MtrunA17_Chr5g0427921 49.057 53 27 0 1 53 1 53 1.00e-13 65.5
Msa0725910 MtrunA17_Chr5g0404951 37.795 127 68 4 1 120 1 123 2.17e-13 68.9
Msa0725910 MtrunA17_Chr8g0351051 52.830 53 25 0 1 53 1 53 3.27e-13 68.9
Msa0725910 MtrunA17_Chr4g0049541 45.333 75 39 1 1 75 1 73 3.39e-13 68.2
Msa0725910 MtrunA17_Chr6g0455511 29.444 180 101 4 1 180 1 154 4.54e-13 68.6
Msa0725910 MtrunA17_Chr4g0074101 50.000 54 27 0 1 54 1 54 4.83e-13 67.4
Msa0725910 MtrunA17_Chr2g0308251 47.170 53 28 0 1 53 1 53 6.48e-13 63.9
Msa0725910 MtrunA17_Chr5g0420081 52.830 53 25 0 1 53 1 53 6.64e-13 67.8
Msa0725910 MtrunA17_Chr1g0180591 51.852 54 26 0 1 54 1 54 7.67e-13 66.2
Msa0725910 MtrunA17_Chr1g0175601 50.000 54 27 0 1 54 1 54 7.89e-13 66.2
Msa0725910 MtrunA17_Chr8g0376821 48.148 54 28 0 1 54 1 54 8.86e-13 66.2
Msa0725910 MtrunA17_Chr8g0357771 30.827 133 65 2 1 133 1 106 1.21e-12 65.9
Msa0725910 MtrunA17_Chr4g0061691 49.057 53 27 0 1 53 1 53 1.38e-12 66.6
Msa0725910 MtrunA17_Chr4g0057151 29.508 183 97 5 1 180 1 154 1.59e-12 65.9
Msa0725910 MtrunA17_Chr1g0164791 37.662 77 48 0 1 77 1 77 1.90e-12 66.2
Msa0725910 MtrunA17_Chr1g0169761 48.148 54 28 0 1 54 1 54 2.18e-12 65.1
Msa0725910 MtrunA17_Chr8g0366231 37.975 79 48 1 1 79 1 78 2.64e-12 65.9
Msa0725910 MtrunA17_Chr8g0356071 30.769 130 63 3 1 130 1 103 2.67e-12 64.3
Msa0725910 MtrunA17_Chr5g0411691 32.117 137 73 4 1 123 1 131 2.89e-12 65.9
Msa0725910 MtrunA17_Chr4g0052061 48.148 54 28 0 1 54 1 54 4.28e-12 63.5
Msa0725910 MtrunA17_Chr8g0379021 28.750 160 78 5 2 135 12 161 4.38e-12 64.3
Msa0725910 MtrunA17_Chr1g0212151 28.972 107 69 2 1 101 1 106 7.44e-12 65.9
Msa0725910 MtrunA17_Chr6g0457851 25.000 152 96 4 1 135 11 161 1.01e-11 65.5
Msa0725910 MtrunA17_Chr6g0474371 47.170 53 28 0 1 53 1 53 2.09e-11 62.4
Msa0725910 MtrunA17_Chr4g0049901 50.943 53 26 0 1 53 27 79 2.23e-11 63.2
Msa0725910 MtrunA17_Chr2g0333981 45.283 53 29 0 1 53 11 63 2.56e-11 62.0
Msa0725910 MtrunA17_Chr4g0052081 50.000 54 27 0 1 54 1 54 3.22e-11 61.6
Msa0725910 MtrunA17_Chr4g0018421 42.105 76 41 2 1 75 1 74 3.82e-11 62.0
Msa0725910 MtrunA17_Chr1g0204111 41.096 73 41 1 1 73 17 87 4.49e-11 62.8
Msa0725910 MtrunA17_Chr3g0099421 41.429 70 39 1 2 71 34 101 5.24e-11 62.4
Msa0725910 MtrunA17_Chr1g0208581 41.096 73 41 1 2 74 9 79 6.44e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0725910 AT2G03060.2 46.253 387 164 8 1 354 1 376 4.68e-100 301
Msa0725910 AT2G03060.3 46.292 391 166 7 1 358 1 380 4.86e-100 301
Msa0725910 AT2G03060.7 46.292 391 166 7 1 358 1 380 4.86e-100 301
Msa0725910 AT2G03060.1 46.292 391 166 7 1 358 1 380 4.86e-100 301
Msa0725910 AT1G18750.4 53.962 265 110 5 1 257 1 261 1.91e-86 266
Msa0725910 AT1G18750.1 53.962 265 110 5 1 257 1 261 1.99e-86 266
Msa0725910 AT1G69540.1 43.681 364 169 13 1 352 1 340 3.43e-81 251
Msa0725910 AT1G69540.2 43.681 364 169 13 1 352 1 340 9.26e-81 251
Msa0725910 AT1G18750.3 47.987 298 110 6 1 257 1 294 2.14e-79 249
Msa0725910 AT1G18750.2 47.945 219 102 5 47 257 1 215 1.15e-56 188
Msa0725910 AT2G03060.6 39.405 269 124 6 82 322 24 281 5.69e-48 165
Msa0725910 AT2G03060.5 39.405 269 124 6 82 322 24 281 5.69e-48 165
Msa0725910 AT2G03060.4 39.405 269 124 6 82 322 24 281 5.69e-48 165
Msa0725910 AT1G77980.1 35.533 197 111 6 1 182 1 196 6.04e-28 112
Msa0725910 AT1G69540.3 32.714 269 146 12 96 352 2 247 2.67e-26 105
Msa0725910 AT1G22130.1 32.653 196 117 5 1 182 1 195 2.71e-25 105
Msa0725910 AT1G18750.5 38.312 154 83 5 112 257 10 159 2.62e-23 98.6
Msa0725910 AT1G77950.3 34.225 187 106 6 1 171 1 186 1.11e-22 96.3
Msa0725910 AT1G77950.2 34.225 187 106 6 1 171 1 186 1.11e-22 96.3
Msa0725910 AT1G77950.1 34.225 187 106 6 1 171 1 186 1.11e-22 96.3
Msa0725910 AT1G77950.4 34.225 187 106 6 1 171 1 186 1.11e-22 96.3
Msa0725910 AT1G18750.6 37.255 153 84 5 113 257 23 171 1.68e-21 93.6
Msa0725910 AT2G26320.1 43.902 82 46 0 1 82 17 98 6.10e-18 79.0
Msa0725910 AT4G37435.1 28.421 190 100 6 1 172 1 172 2.71e-17 79.3
Msa0725910 AT3G57390.3 32.571 175 91 6 1 171 1 152 1.06e-16 79.0
Msa0725910 AT3G57390.1 32.571 175 91 6 1 171 1 152 1.41e-16 79.0
Msa0725910 AT5G13790.1 37.374 99 58 2 1 97 1 97 6.55e-15 74.3
Msa0725910 AT5G13790.2 54.717 53 24 0 1 53 1 53 8.08e-15 74.3
Msa0725910 AT4G09960.2 54.717 53 24 0 1 53 1 53 1.02e-14 72.8
Msa0725910 AT4G09960.6 54.717 53 24 0 1 53 2 54 1.27e-14 73.2
Msa0725910 AT4G09960.1 54.717 53 24 0 1 53 1 53 1.27e-14 72.8
Msa0725910 AT2G03710.2 31.183 186 101 4 1 185 1 160 1.52e-14 73.2
Msa0725910 AT2G03710.1 31.183 186 101 4 1 185 1 160 1.66e-14 73.2
Msa0725910 AT4G09960.5 54.717 53 24 0 1 53 11 63 1.76e-14 72.8
Msa0725910 AT2G03710.3 60.377 53 21 0 1 53 1 53 1.78e-14 71.6
Msa0725910 AT4G09960.3 54.717 53 24 0 1 53 27 79 2.37e-14 72.8
Msa0725910 AT3G58780.2 54.717 53 24 0 1 53 16 68 2.66e-14 72.4
Msa0725910 AT3G58780.1 54.717 53 24 0 1 53 16 68 3.14e-14 72.0
Msa0725910 AT3G58780.5 54.717 53 24 0 1 53 22 74 3.28e-14 72.4
Msa0725910 AT2G42830.1 54.717 53 24 0 1 53 16 68 3.30e-14 72.0
Msa0725910 AT2G42830.2 54.717 53 24 0 1 53 16 68 3.36e-14 72.0
Msa0725910 AT2G45660.2 28.729 181 101 3 1 180 1 154 3.58e-14 70.1
Msa0725910 AT3G61120.1 56.604 53 23 0 1 53 1 53 3.92e-14 72.0
Msa0725910 AT3G58780.3 54.717 53 24 0 1 53 16 68 4.47e-14 72.0
Msa0725910 AT3G58780.4 54.717 53 24 0 1 53 16 68 4.81e-14 72.0
Msa0725910 AT2G45660.1 29.834 181 99 5 1 180 1 154 4.82e-14 70.9
Msa0725910 AT4G11880.2 54.717 53 24 0 1 53 1 53 5.40e-14 70.1
Msa0725910 AT4G11880.3 54.717 53 24 0 1 53 1 53 5.40e-14 70.1
Msa0725910 AT4G09960.4 54.717 53 24 0 1 53 96 148 5.86e-14 72.4
Msa0725910 AT4G18960.1 55.769 52 23 0 2 53 18 69 6.30e-14 71.2
Msa0725910 AT5G10140.2 54.717 53 24 0 1 53 1 53 7.97e-14 69.3
Msa0725910 AT5G10140.3 54.717 53 24 0 1 53 1 53 8.45e-14 69.7
Msa0725910 AT5G10140.1 54.717 53 24 0 1 53 1 53 1.16e-13 69.3
Msa0725910 AT4G22950.3 29.189 185 102 5 1 184 1 157 1.17e-13 70.1
Msa0725910 AT4G22950.1 29.189 185 102 5 1 184 1 157 1.17e-13 70.1
Msa0725910 AT5G10140.4 54.717 53 24 0 1 53 1 53 1.32e-13 68.9
Msa0725910 AT4G11880.4 54.717 53 24 0 1 53 1 53 1.36e-13 69.7
Msa0725910 AT4G11880.1 54.717 53 24 0 1 53 1 53 1.36e-13 69.7
Msa0725910 AT4G24540.1 52.830 53 25 0 1 53 1 53 1.76e-13 69.3
Msa0725910 AT2G34440.1 50.000 54 27 0 1 54 1 54 2.08e-13 68.2
Msa0725910 AT3G57230.2 50.943 53 26 0 1 53 1 53 2.19e-13 67.4
Msa0725910 AT5G20240.2 50.943 53 26 0 1 53 1 53 2.40e-13 67.4
Msa0725910 AT1G24260.3 54.717 53 24 0 1 53 1 53 2.80e-13 69.3
Msa0725910 AT3G57230.1 43.056 72 39 1 1 72 1 70 2.84e-13 69.3
Msa0725910 AT3G57230.4 43.056 72 39 1 1 72 1 70 2.84e-13 69.3
Msa0725910 AT1G24260.2 54.717 53 24 0 1 53 1 53 3.42e-13 69.3
Msa0725910 AT1G24260.1 54.717 53 24 0 1 53 1 53 3.51e-13 69.3
Msa0725910 AT4G22950.2 29.189 185 102 5 1 184 1 157 3.63e-13 67.4
Msa0725910 AT5G15800.1 54.717 53 24 0 1 53 1 53 4.02e-13 68.9
Msa0725910 AT2G45650.1 52.830 53 25 0 1 53 1 53 5.07e-13 68.6
Msa0725910 AT3G02310.2 54.717 53 24 0 1 53 1 53 5.35e-13 67.8
Msa0725910 AT5G15800.2 54.717 53 24 0 1 53 1 53 5.68e-13 68.9
Msa0725910 AT5G20240.1 46.154 65 29 1 1 59 1 65 5.73e-13 67.8
Msa0725910 AT1G71692.1 43.210 81 43 2 1 80 1 79 7.16e-13 67.4
Msa0725910 AT5G23260.3 43.662 71 40 0 1 71 1 71 7.18e-13 68.2
Msa0725910 AT5G23260.2 43.662 71 40 0 1 71 1 71 7.21e-13 68.2
Msa0725910 AT3G66656.1 40.845 71 40 1 1 71 1 69 7.54e-13 66.6
Msa0725910 AT5G65070.4 50.943 53 26 0 1 53 1 53 7.99e-13 65.9
Msa0725910 AT2G22540.3 42.254 71 37 2 1 70 1 68 8.76e-13 67.8
Msa0725910 AT2G22540.1 42.254 71 37 2 1 70 1 68 8.76e-13 67.8
Msa0725910 AT5G23260.1 43.662 71 40 0 1 71 17 87 9.40e-13 68.2
Msa0725910 AT5G23260.4 43.662 71 40 0 1 71 17 87 9.76e-13 68.2
Msa0725910 AT3G30260.1 49.057 53 27 0 1 53 1 53 9.95e-13 67.8
Msa0725910 AT3G02310.1 54.717 53 24 0 1 53 1 53 9.98e-13 67.8
Msa0725910 AT2G22540.2 49.057 53 27 0 1 53 1 53 1.24e-12 67.4
Msa0725910 AT5G65050.2 52.830 53 25 0 1 53 1 53 1.43e-12 65.9
Msa0725910 AT3G54340.1 52.830 53 25 0 1 53 1 53 1.44e-12 67.0
Msa0725910 AT1G26310.1 39.241 79 47 1 1 79 1 78 1.55e-12 67.4
Msa0725910 AT5G65070.1 50.943 53 26 0 1 53 1 53 1.75e-12 66.2
Msa0725910 AT5G62165.14 49.057 53 27 0 1 53 1 53 2.35e-12 63.5
Msa0725910 AT2G14210.1 50.943 53 26 0 1 53 1 53 2.43e-12 66.6
Msa0725910 AT5G62165.17 26.630 184 105 5 1 182 1 156 2.68e-12 64.7
Msa0725910 AT5G62165.12 26.630 184 105 5 1 182 1 156 2.68e-12 64.7
Msa0725910 AT5G62165.11 26.630 184 105 5 1 182 1 156 2.68e-12 64.7
Msa0725910 AT2G14210.2 50.943 53 26 0 1 53 1 53 2.82e-12 66.2
Msa0725910 AT5G65070.5 50.943 53 26 0 1 53 1 53 2.94e-12 65.9
Msa0725910 AT5G60910.1 49.057 53 27 0 1 53 1 53 3.70e-12 66.2
Msa0725910 AT5G65070.2 50.943 53 26 0 1 53 1 53 4.01e-12 65.9
Msa0725910 AT5G65070.3 50.943 53 26 0 1 53 1 53 4.07e-12 65.9
Msa0725910 AT5G62165.3 26.344 186 107 5 1 184 1 158 4.32e-12 65.5
Msa0725910 AT5G62165.1 26.344 186 107 5 1 184 1 158 4.32e-12 65.5
Msa0725910 AT5G62165.2 26.344 186 107 5 1 184 1 158 4.32e-12 65.5
Msa0725910 AT1G69120.2 37.975 79 48 1 1 79 1 78 5.15e-12 65.5
Msa0725910 AT5G62165.6 40.000 75 43 1 1 75 1 73 5.17e-12 63.9
Msa0725910 AT5G62165.9 40.000 75 43 1 1 75 1 73 5.17e-12 63.9
Msa0725910 AT5G62165.7 40.000 75 43 1 1 75 1 73 5.17e-12 63.9
Msa0725910 AT5G62165.8 40.000 75 43 1 1 75 1 73 5.17e-12 63.9
Msa0725910 AT5G62165.13 40.000 75 43 1 1 75 1 73 5.17e-12 63.9
Msa0725910 AT5G65050.3 52.830 53 25 0 1 53 1 53 5.99e-12 64.7
Msa0725910 AT4G37940.1 49.057 53 27 0 1 53 1 53 6.25e-12 65.1
Msa0725910 AT5G65050.1 52.830 53 25 0 1 53 1 53 6.26e-12 64.3
Msa0725910 AT5G65050.5 52.830 53 25 0 1 53 1 53 6.29e-12 64.3
Msa0725910 AT4G37940.4 49.057 53 27 0 1 53 1 53 6.54e-12 64.7
Msa0725910 AT4G37940.3 49.057 53 27 0 1 53 1 53 6.67e-12 63.9
Msa0725910 AT5G62165.10 28.671 143 91 3 1 134 1 141 7.26e-12 64.3
Msa0725910 AT5G62165.4 28.671 143 91 3 1 134 1 141 7.26e-12 64.3
Msa0725910 AT1G69120.1 37.975 79 48 1 1 79 1 78 9.50e-12 65.1
Msa0725910 AT5G51870.2 35.238 105 59 4 1 98 1 103 1.36e-11 63.2
Msa0725910 AT5G65060.1 28.889 180 95 4 1 180 1 147 1.53e-11 63.5
Msa0725910 AT5G65060.3 28.889 180 95 4 1 180 1 147 2.01e-11 62.8
Msa0725910 AT5G65080.3 50.943 53 26 0 1 53 8 60 2.66e-11 62.8
Msa0725910 AT5G65080.2 50.943 53 26 0 1 53 8 60 2.87e-11 62.8
Msa0725910 AT5G51870.1 38.667 75 44 1 1 75 1 73 4.12e-11 62.4
Msa0725910 AT5G51870.5 35.238 105 59 4 1 98 24 126 5.19e-11 62.0
Msa0725910 AT1G47760.1 35.165 91 57 1 1 91 1 89 8.21e-11 61.2

Find 57 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAGTAGTGTGGATAAAT+TGG 0.192371 5_1:+26001128 Msa0725910:CDS
CATTATTCCATTCTTTCTTT+TGG 0.211124 5_1:-25998541 None:intergenic
ATTGAATTGTAACAGGAAAT+TGG 0.220242 5_1:+26000005 Msa0725910:intron
GTTAGACTACAACAATTTAA+TGG 0.235139 5_1:+26003162 Msa0725910:CDS
CCGAAATTCATGAGAGATTA+AGG 0.265694 5_1:+26000955 Msa0725910:CDS
TTAAATTGTTGTAGTCTAAC+TGG 0.296546 5_1:-26003159 None:intergenic
GCAGCTTTCTTGACCAGAAT+TGG 0.312176 5_1:-26003103 None:intergenic
CCTTAATCTCTCATGAATTT+CGG 0.312515 5_1:-26000955 None:intergenic
TTGAAATTGTTTGGTATGTA+TGG 0.317670 5_1:-26003192 None:intergenic
TTCATAATGTTGAAATTGTT+TGG 0.322241 5_1:-26003201 None:intergenic
AAAACAAACATGTCCAATTC+TGG 0.323546 5_1:+26003090 Msa0725910:CDS
GGAAGCTATGCTAGTTATCT+TGG 0.323605 5_1:+26003057 Msa0725910:CDS
TCTCTGTCTTGGATGGTTAA+TGG 0.333794 5_1:+26002059 Msa0725910:CDS
ATGAAGCTCCAACCAGTTAC+AGG 0.341157 5_1:+26003228 Msa0725910:CDS
CATGCTTGGACTTCTAATTC+AGG 0.370657 5_1:+26003345 Msa0725910:CDS
GACTGATCAAACCCTTGTTT+AGG 0.371489 5_1:-26003312 None:intergenic
GGGTAAAGCTAAAGATAAAA+AGG 0.374410 5_1:+25998484 Msa0725910:CDS
ATCATTGCAATGCAATAATC+AGG 0.385364 5_1:+26001887 Msa0725910:CDS
TGATGAACATTTGTATCCTC+AGG 0.394325 5_1:+26003389 Msa0725910:CDS
GGATTATTCATCCCTGTAAC+TGG 0.398800 5_1:-26003240 None:intergenic
GGCTCAACAAGCTCATCATT+TGG 0.399741 5_1:+26003036 Msa0725910:CDS
ATTAGTAGTGTGGATAAATT+GGG 0.417546 5_1:+26001129 Msa0725910:CDS
CTCAACTAACTTCACAAGAA+AGG 0.420598 5_1:+25999891 Msa0725910:CDS
AGAAGCAAACTTGAGTCTTC+AGG 0.422149 5_1:-26002104 None:intergenic
TGGTGAATGTAGCAACTTTG+AGG 0.431835 5_1:+25999850 Msa0725910:intron
AGCATCTTCAATCTCTGTCT+TGG 0.441721 5_1:+26002048 Msa0725910:CDS
ACCTATGCCAAAAGAAAGAA+TGG 0.450298 5_1:+25998534 Msa0725910:CDS
TAATCAAATTCAAACTCATA+AGG 0.457761 5_1:+26001182 Msa0725910:CDS
TGTGGATAAATTGGGACAGA+TGG 0.483660 5_1:+26001137 Msa0725910:CDS
AAATGTACAAAAGCAACAAC+TGG 0.484094 5_1:+26001863 Msa0725910:CDS
AAAAGGTTGGAGAACACAAA+TGG 0.486098 5_1:+25998501 Msa0725910:CDS
CTTCAGGTAACACTAAATTC+TGG 0.489233 5_1:-26002088 None:intergenic
AGTCTAATAATCACCATGCT+TGG 0.503117 5_1:+26003331 Msa0725910:CDS
TTCTATTATAGAGATGTAGA+AGG 0.503989 5_1:+26003015 Msa0725910:intron
CGCCTCCCTGTGCATAATGA+AGG 0.509179 5_1:-25998636 None:intergenic
TCAACTAACTTCACAAGAAA+GGG 0.510639 5_1:+25999892 Msa0725910:CDS
CATTATGCACAGGGAGGCGC+AGG 0.512848 5_1:+25998640 Msa0725910:CDS
TCTTCAATCTCTGTCTTGGA+TGG 0.532373 5_1:+26002052 Msa0725910:CDS
GATACGCTTATGTAAAAGCT+TGG 0.535960 5_1:-26000931 None:intergenic
GGAAAACCTTCATTATGCAC+AGG 0.541969 5_1:+25998630 Msa0725910:CDS
TATTCATCCCTGTAACTGGT+TGG 0.552728 5_1:-26003236 None:intergenic
GATGAACATTTGTATCCTCA+GGG 0.556930 5_1:+26003390 Msa0725910:CDS
TATGAATTTGTAGGATGGGG+AGG 0.564718 5_1:+25998463 None:intergenic
TTATCTACTTGAGTTTCATG+TGG 0.567505 5_1:-26003261 None:intergenic
GTGAATCCAAAATGCACTTG+TGG 0.579613 5_1:-26003420 None:intergenic
TTGCAGTAGTCAAACTGTTG+AGG 0.584893 5_1:+26000814 Msa0725910:intron
ATGAATTTGTAGGATGGGGA+GGG 0.596204 5_1:+25998464 None:intergenic
CTTCACAAGAAAGGGCAAAG+AGG 0.603770 5_1:+25999900 Msa0725910:CDS
CTGAATTAGAAGTCCAAGCA+TGG 0.625702 5_1:-26003344 None:intergenic
TGAAGCTCCAACCAGTTACA+GGG 0.625859 5_1:+26003229 Msa0725910:CDS
ACAGATGGAAAATTCACTCA+AGG 0.626422 5_1:+26001152 Msa0725910:CDS
ACAGAGATTGAAGATGCTGT+TGG 0.653260 5_1:-26002043 None:intergenic
TCAAAGATATTAACAGCACA+AGG 0.669359 5_1:-26003369 None:intergenic
GAAAACCTTCATTATGCACA+GGG 0.671432 5_1:+25998631 Msa0725910:CDS
AACCTTCATTATGCACAGGG+AGG 0.717217 5_1:+25998634 Msa0725910:CDS
TGAATGTAGCAACTTTGAGG+AGG 0.731003 5_1:+25999853 Msa0725910:intron
ACTTGTGGAAAATTTCCCTG+AGG 0.756884 5_1:-26003405 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATATGTTTATTTTACA+AGG + chr5_1:25999790-25999809 Msa0725910:intron 10.0%
!! GTTAAAAAGTAAATAAAAAT+TGG - chr5_1:26000622-26000641 None:intergenic 10.0%
!!! TAAGTTTTTTTCTTTTTAAA+CGG + chr5_1:26001636-26001655 Msa0725910:intron 10.0%
!! ATGATATAAAAATAAAAACA+TGG + chr5_1:26001963-26001982 Msa0725910:intron 10.0%
!! TATGTAACAATTAATGTAAA+TGG + chr5_1:25998687-25998706 Msa0725910:intron 15.0%
!! ATGTAACAATTAATGTAAAT+GGG + chr5_1:25998688-25998707 Msa0725910:intron 15.0%
!!! TTTCAATTCTAAGTTTTAAT+GGG + chr5_1:25998945-25998964 Msa0725910:intron 15.0%
!! TTGTAATAGTAATAATTCAT+GGG - chr5_1:25999302-25999321 None:intergenic 15.0%
!! TTTGTAATAGTAATAATTCA+TGG - chr5_1:25999303-25999322 None:intergenic 15.0%
!! TAATAAGTAAAACTAACATT+AGG - chr5_1:25999775-25999794 None:intergenic 15.0%
!! TTATCAAATTCTAATGTATT+AGG - chr5_1:25999974-25999993 None:intergenic 15.0%
!!! TGATTTTCTTAAATTATTAG+CGG - chr5_1:26000133-26000152 None:intergenic 15.0%
!! TTCAATATAATTATATGTGT+TGG + chr5_1:26000158-26000177 Msa0725910:intron 15.0%
!! AAAGTAAATAAAAATTGGTT+GGG - chr5_1:26000617-26000636 None:intergenic 15.0%
!! AAAAGTAAATAAAAATTGGT+TGG - chr5_1:26000618-26000637 None:intergenic 15.0%
!! TTAAGTTTATCTTCTAAATT+CGG - chr5_1:26001752-26001771 None:intergenic 15.0%
!!! GAAAATTGATATATTTTCAT+TGG - chr5_1:26002484-26002503 None:intergenic 15.0%
!! TTAATACAAATGAAATGTAA+AGG - chr5_1:26002710-26002729 None:intergenic 15.0%
!!! CTTTCAATTCTAAGTTTTAA+TGG + chr5_1:25998944-25998963 Msa0725910:intron 20.0%
!!! TTCAATTCTAAGTTTTAATG+GGG + chr5_1:25998946-25998965 Msa0725910:intron 20.0%
!!! TTTTAAGAATACATTAGAGA+GGG - chr5_1:25999061-25999080 None:intergenic 20.0%
!!! TTTTTAAGAATACATTAGAG+AGG - chr5_1:25999062-25999081 None:intergenic 20.0%
!!! AAAATTGTGTTTTTCTCATA+CGG + chr5_1:25999161-25999180 Msa0725910:intron 20.0%
!!! ATTAGCATAGTTTTAGAAAA+AGG + chr5_1:25999253-25999272 Msa0725910:intron 20.0%
!! TACAAACAAAATTCTAACAT+TGG + chr5_1:25999317-25999336 Msa0725910:intron 20.0%
!!! TTATATTTGACGTTTTATCA+CGG + chr5_1:25999513-25999532 Msa0725910:intron 20.0%
!!! TATTATTATTAGAGCATTCA+AGG + chr5_1:25999740-25999759 Msa0725910:intron 20.0%
!!! TAGTAATGATTTTTTGTTTG+AGG + chr5_1:26000318-26000337 Msa0725910:intron 20.0%
!! AATGAGAAAATGAGAAAAAA+AGG - chr5_1:26000857-26000876 None:intergenic 20.0%
!! TAATCAAATTCAAACTCATA+AGG + chr5_1:26001182-26001201 Msa0725910:CDS 20.0%
!! AATGTCTTCAATTTATTTAG+TGG + chr5_1:26001228-26001247 Msa0725910:intron 20.0%
!!! TTTGTTCAATTTGTTTATAC+AGG - chr5_1:26001344-26001363 None:intergenic 20.0%
!! TTTCTTTAAGCAAAAAAATC+AGG + chr5_1:26001395-26001414 Msa0725910:intron 20.0%
!! AGTTTATGAAAACAACTTAT+AGG - chr5_1:26001553-26001572 None:intergenic 20.0%
!! TAAGTTTATCTTCTAAATTC+GGG - chr5_1:26001751-26001770 None:intergenic 20.0%
!! ATTTAAATGCTACATTATTG+AGG - chr5_1:26001813-26001832 None:intergenic 20.0%
!!! ATCAGGTAAATAATTGTTTT+AGG + chr5_1:26001904-26001923 Msa0725910:intron 20.0%
!!! TCAGGTAAATAATTGTTTTA+GGG + chr5_1:26001905-26001924 Msa0725910:intron 20.0%
!! ACTCAAAAATTAGAATAACA+AGG - chr5_1:26002185-26002204 None:intergenic 20.0%
!!! TTGAGTTTGCTTAATTATTT+TGG + chr5_1:26002199-26002218 Msa0725910:intron 20.0%
!! AATTATTTCAGTTACAACAT+AGG + chr5_1:26002239-26002258 Msa0725910:intron 20.0%
!! ATCATGTAATAAACATTATC+CGG + chr5_1:26002289-26002308 Msa0725910:intron 20.0%
!!! TTATTTTGTTTTGAAAACTC+AGG - chr5_1:26002412-26002431 None:intergenic 20.0%
!!! TTAATTTAGTCCACATTTTT+AGG + chr5_1:26002606-26002625 Msa0725910:intron 20.0%
!!! TAATTTAGTCCACATTTTTA+GGG + chr5_1:26002607-26002626 Msa0725910:intron 20.0%
!! TTTGTCATATTTAATGATGT+TGG - chr5_1:26002640-26002659 None:intergenic 20.0%
!! ACATAATTTAGTGTAATCAT+AGG - chr5_1:26002817-26002836 None:intergenic 20.0%
!!! TTAATCAATAAAGTTGATCA+TGG + chr5_1:26002919-26002938 Msa0725910:intron 20.0%
!! TTCATAATGTTGAAATTGTT+TGG - chr5_1:26003204-26003223 None:intergenic 20.0%
! AAAGCTAAAGATAAAAAGGT+TGG + chr5_1:25998488-25998507 Msa0725910:CDS 25.0%
!! CATTATTCCATTCTTTCTTT+TGG - chr5_1:25998544-25998563 None:intergenic 25.0%
! ATGTTTGATAGAACACAAAT+AGG + chr5_1:25998833-25998852 Msa0725910:intron 25.0%
! TGTTTGATAGAACACAAATA+GGG + chr5_1:25998834-25998853 Msa0725910:intron 25.0%
! ACGGTCATATTATAATGATT+TGG - chr5_1:25999346-25999365 None:intergenic 25.0%
! TATCTAACTGCTAAAAATCA+CGG - chr5_1:25999365-25999384 None:intergenic 25.0%
!! TATATTTGACGTTTTATCAC+GGG + chr5_1:25999514-25999533 Msa0725910:intron 25.0%
! TATCAATTCTTATGTGTGTT+TGG - chr5_1:25999696-25999715 None:intergenic 25.0%
! TACAAATATTCAAAGCAATC+AGG - chr5_1:25999722-25999741 None:intergenic 25.0%
! ATTTCCTGTTACAATTCAAT+TGG - chr5_1:26000005-26000024 None:intergenic 25.0%
! ATTGAATTGTAACAGGAAAT+TGG + chr5_1:26000005-26000024 Msa0725910:intron 25.0%
!!! TCATACATCTTTCTCTTTTT+TGG + chr5_1:26000262-26000281 Msa0725910:intron 25.0%
! ATCATTACTAAGAATGAATG+TGG - chr5_1:26000308-26000327 None:intergenic 25.0%
!! GTGAATATACAAGACTTTTA+TGG + chr5_1:26000381-26000400 Msa0725910:CDS 25.0%
!! TTTTCGAAACATTGATATTC+AGG - chr5_1:26000446-26000465 None:intergenic 25.0%
! AATATCAATGTTTCGAAAAC+CGG + chr5_1:26000447-26000466 Msa0725910:intron 25.0%
! CATTGAAAAAATTAGTAGTG+TGG + chr5_1:26001119-26001138 Msa0725910:CDS 25.0%
! AATTAGTAGTGTGGATAAAT+TGG + chr5_1:26001128-26001147 Msa0725910:CDS 25.0%
! ATTAGTAGTGTGGATAAATT+GGG + chr5_1:26001129-26001148 Msa0725910:CDS 25.0%
! AACAAATTGAACAAACTTGA+AGG + chr5_1:26001349-26001368 Msa0725910:intron 25.0%
!!! AAAAATCAGGTTTTGATGTT+TGG + chr5_1:26001408-26001427 Msa0725910:intron 25.0%
! TTCAATTGTTTATCCAAACA+AGG - chr5_1:26001456-26001475 None:intergenic 25.0%
! AAAACCATACAACAAAATCT+CGG + chr5_1:26001725-26001744 Msa0725910:intron 25.0%
! TTAAATAAGTCAATCCAAAC+AGG + chr5_1:26001768-26001787 Msa0725910:intron 25.0%
! ATAGGCAAATAAAATCATAG+TGG + chr5_1:26002257-26002276 Msa0725910:intron 25.0%
!!! GTAAAGACAAGTTTTTGTTT+TGG + chr5_1:26002517-26002536 Msa0725910:intron 25.0%
! ATAACTCATGACTATATGTA+AGG - chr5_1:26002575-26002594 None:intergenic 25.0%
! TCTACATCTCTATAATAGAA+GGG - chr5_1:26003015-26003034 None:intergenic 25.0%
! TTCTACATCTCTATAATAGA+AGG - chr5_1:26003016-26003035 None:intergenic 25.0%
! TTCTATTATAGAGATGTAGA+AGG + chr5_1:26003015-26003034 Msa0725910:intron 25.0%
! TTAAATTGTTGTAGTCTAAC+TGG - chr5_1:26003162-26003181 None:intergenic 25.0%
! GTTAGACTACAACAATTTAA+TGG + chr5_1:26003162-26003181 Msa0725910:CDS 25.0%
! TTGAAATTGTTTGGTATGTA+TGG - chr5_1:26003195-26003214 None:intergenic 25.0%
GGGTAAAGCTAAAGATAAAA+AGG + chr5_1:25998484-25998503 Msa0725910:CDS 30.0%
! ATACTTCTTATGTTTTCTCC+AGG + chr5_1:25998606-25998625 Msa0725910:CDS 30.0%
! TTTGTTGTGGACATAATTGA+TGG + chr5_1:25998867-25998886 Msa0725910:intron 30.0%
CAAGCAATTTCATAAGATGA+TGG + chr5_1:25998979-25998998 Msa0725910:intron 30.0%
TAGACAAATGTGAGAAAAGA+CGG - chr5_1:25999125-25999144 None:intergenic 30.0%
! ATACGGATTGACAAATTTAG+AGG + chr5_1:25999178-25999197 Msa0725910:intron 30.0%
!! GATCGATTTTTATAAGCCTA+AGG + chr5_1:25999202-25999221 Msa0725910:intron 30.0%
! GCATATGTTTGGATTTTCTT+GGG - chr5_1:25999425-25999444 None:intergenic 30.0%
! AGCATATGTTTGGATTTTCT+TGG - chr5_1:25999426-25999445 None:intergenic 30.0%
AAGAACACTTAGCATATGTT+TGG - chr5_1:25999436-25999455 None:intergenic 30.0%
AAACACACATATAGCATGTT+TGG - chr5_1:25999583-25999602 None:intergenic 30.0%
AAACATGCTATATGTGTGTT+TGG + chr5_1:25999582-25999601 Msa0725910:intron 30.0%
! AAGCTACAAAAAAGTGTGAA+TGG + chr5_1:25999660-25999679 Msa0725910:intron 30.0%
AATTATTGTCTCCTCTACTT+TGG + chr5_1:25999830-25999849 Msa0725910:intron 30.0%
TCAACTAACTTCACAAGAAA+GGG + chr5_1:25999892-25999911 Msa0725910:CDS 30.0%
TTCTCCAATTGAATTGTAAC+AGG + chr5_1:25999998-26000017 Msa0725910:intron 30.0%
!!! ATGATTTTTTGTTTGAGGTC+AGG + chr5_1:26000323-26000342 Msa0725910:intron 30.0%
! TACAAGACTTTTATGGTACA+AGG + chr5_1:26000388-26000407 Msa0725910:CDS 30.0%
AAACCAACAAGCAAATTGAT+TGG - chr5_1:26000508-26000527 None:intergenic 30.0%
ATACTGCAATCTAGTTGAAT+CGG + chr5_1:26000538-26000557 Msa0725910:intron 30.0%
GAGAAAAAAAGGAGTAATTC+AGG - chr5_1:26000846-26000865 None:intergenic 30.0%
CCTTAATCTCTCATGAATTT+CGG - chr5_1:26000958-26000977 None:intergenic 30.0%
! TTGTTGAATTTTGCAGCAAT+TGG + chr5_1:26001091-26001110 Msa0725910:intron 30.0%
! GACATTTTTCATAGTGTATC+AGG - chr5_1:26001214-26001233 None:intergenic 30.0%
GACACAGAAAAGTTGTATTT+AGG + chr5_1:26001430-26001449 Msa0725910:intron 30.0%
CATTATTGAGGATAACATCT+AGG - chr5_1:26001801-26001820 None:intergenic 30.0%
! GTACATTTTCCTATCAAAGT+AGG - chr5_1:26001851-26001870 None:intergenic 30.0%
AAATGTACAAAAGCAACAAC+TGG + chr5_1:26001863-26001882 Msa0725910:CDS 30.0%
ATCATTGCAATGCAATAATC+AGG + chr5_1:26001887-26001906 Msa0725910:CDS 30.0%
AAGAAAAAACAACTCACTTG+TGG - chr5_1:26002130-26002149 None:intergenic 30.0%
TATTCCATTCAGAGCTTAAA+TGG + chr5_1:26002329-26002348 Msa0725910:intron 30.0%
! TTTCTCTGTCTAAAACTTAC+AGG + chr5_1:26002442-26002461 Msa0725910:intron 30.0%
!! GGTTTTAATCCCTAAAAATG+TGG - chr5_1:26002619-26002638 None:intergenic 30.0%
! ATCAATAAAGTTGATCATGG+TGG + chr5_1:26002922-26002941 Msa0725910:intron 30.0%
AAAACAAACATGTCCAATTC+TGG + chr5_1:26003090-26003109 Msa0725910:CDS 30.0%
TTATCTACTTGAGTTTCATG+TGG - chr5_1:26003264-26003283 None:intergenic 30.0%
!!! AATTTTGAAGCACCTAAACA+AGG + chr5_1:26003300-26003319 Msa0725910:CDS 30.0%
!!! ATTTTGAAGCACCTAAACAA+GGG + chr5_1:26003301-26003320 Msa0725910:CDS 30.0%
TCAAAGATATTAACAGCACA+AGG - chr5_1:26003372-26003391 None:intergenic 30.0%
! AATTTTCCACAAGTGCATTT+TGG + chr5_1:26003414-26003433 Msa0725910:CDS 30.0%
AAAAGGTTGGAGAACACAAA+TGG + chr5_1:25998501-25998520 Msa0725910:CDS 35.0%
ACCTATGCCAAAAGAAAGAA+TGG + chr5_1:25998534-25998553 Msa0725910:CDS 35.0%
! TCCATTCTTTCTTTTGGCAT+AGG - chr5_1:25998538-25998557 None:intergenic 35.0%
GAAAACCTTCATTATGCACA+GGG + chr5_1:25998631-25998650 Msa0725910:CDS 35.0%
TCTTATGAAATTGCTTGCCT+TGG - chr5_1:25998976-25998995 None:intergenic 35.0%
TCTTAAAAATTGCACTCCTC+CGG + chr5_1:25999073-25999092 Msa0725910:intron 35.0%
! GTCTTTTCTCACATTTGTCT+AGG + chr5_1:25999124-25999143 Msa0725910:intron 35.0%
! CAATTAGATGATGATGCCTT+AGG - chr5_1:25999221-25999240 None:intergenic 35.0%
! CTAAGGCATCATCATCTAAT+TGG + chr5_1:25999219-25999238 Msa0725910:intron 35.0%
TATCACGGGAAAAAGATCAT+AGG + chr5_1:25999528-25999547 Msa0725910:intron 35.0%
ATAGGAACGTAAGATTGTAG+TGG + chr5_1:25999546-25999565 Msa0725910:intron 35.0%
GAGGAGACAATAATTAGACA+AGG - chr5_1:25999825-25999844 None:intergenic 35.0%
CTCAACTAACTTCACAAGAA+AGG + chr5_1:25999891-25999910 Msa0725910:CDS 35.0%
CATTATGCATCTATGAAGCA+TGG + chr5_1:26000060-26000079 Msa0725910:intron 35.0%
TTGCTAAATTAGTTGGAGCT+GGG - chr5_1:26000418-26000437 None:intergenic 35.0%
ATTGCTAAATTAGTTGGAGC+TGG - chr5_1:26000419-26000438 None:intergenic 35.0%
GGACACATTGCTAAATTAGT+TGG - chr5_1:26000425-26000444 None:intergenic 35.0%
GAACCATGATTCAACTACTT+CGG + chr5_1:26000591-26000610 Msa0725910:intron 35.0%
TGGTTCAATCACACTTGAAT+CGG - chr5_1:26000682-26000701 None:intergenic 35.0%
ATATTCTGTCCTTCACTAAC+TGG + chr5_1:26000882-26000901 Msa0725910:intron 35.0%
GATACGCTTATGTAAAAGCT+TGG - chr5_1:26000934-26000953 None:intergenic 35.0%
CCGAAATTCATGAGAGATTA+AGG + chr5_1:26000955-26000974 Msa0725910:CDS 35.0%
AGCTTGCAAGATGTAACTAA+AGG - chr5_1:26001052-26001071 None:intergenic 35.0%
ACAGATGGAAAATTCACTCA+AGG + chr5_1:26001152-26001171 Msa0725910:CDS 35.0%
!! GAGAATCAAAGTTCTGAACT+TGG + chr5_1:26001250-26001269 Msa0725910:intron 35.0%
!! AGTTGTATTTAGGCCTTGTT+TGG + chr5_1:26001440-26001459 Msa0725910:intron 35.0%
! GAGGATAACATCTAGGATAA+AGG - chr5_1:26001794-26001813 None:intergenic 35.0%
!!! TGTTGGTGACTCATTTTGTA+GGG - chr5_1:26002029-26002048 None:intergenic 35.0%
CTTCAGGTAACACTAAATTC+TGG - chr5_1:26002091-26002110 None:intergenic 35.0%
!! ACATTATCCGGTGTCAATTT+TGG + chr5_1:26002301-26002320 Msa0725910:intron 35.0%
!!! TGTTTTGAAAACTCAGGATC+CGG - chr5_1:26002406-26002425 None:intergenic 35.0%
! TGTAAAGGTAGTCTCTAAGA+TGG - chr5_1:26002695-26002714 None:intergenic 35.0%
! AAAAAAGTACAGACACTCCT+CGG + chr5_1:26002732-26002751 Msa0725910:intron 35.0%
TATTATCCTTGTCAGTGTGT+CGG + chr5_1:26002849-26002868 Msa0725910:intron 35.0%
AGTCTAATAATCACCATGCT+TGG + chr5_1:26003331-26003350 Msa0725910:CDS 35.0%
TGATGAACATTTGTATCCTC+AGG + chr5_1:26003389-26003408 Msa0725910:CDS 35.0%
GATGAACATTTGTATCCTCA+GGG + chr5_1:26003390-26003409 Msa0725910:CDS 35.0%
! TTCTTTTGGCATAGGTTGCT+TGG - chr5_1:25998530-25998549 None:intergenic 40.0%
! CTTCTTATGTTTTCTCCAGG+TGG + chr5_1:25998609-25998628 Msa0725910:CDS 40.0%
! CATAATGAAGGTTTTCCACC+TGG - chr5_1:25998627-25998646 None:intergenic 40.0%
GGAAAACCTTCATTATGCAC+AGG + chr5_1:25998630-25998649 Msa0725910:CDS 40.0%
! GGGCATTTACTTGTTTGTTG+TGG + chr5_1:25998854-25998873 Msa0725910:intron 40.0%
! AGAATTGAAAGCGTGTGACA+AGG - chr5_1:25998935-25998954 None:intergenic 40.0%
!! AGTTTTAATGGGGCAAACCA+AGG + chr5_1:25998956-25998975 Msa0725910:intron 40.0%
TGCTACATTCACCAAAGTAG+AGG - chr5_1:25999844-25999863 None:intergenic 40.0%
TGGTGAATGTAGCAACTTTG+AGG + chr5_1:25999850-25999869 Msa0725910:intron 40.0%
! TGAATGTAGCAACTTTGAGG+AGG + chr5_1:25999853-25999872 Msa0725910:intron 40.0%
GCATCTATGAAGCATGGATA+CGG + chr5_1:26000066-26000085 Msa0725910:intron 40.0%
!! TATATGTGTTGGTGTCGTGT+CGG + chr5_1:26000169-26000188 Msa0725910:intron 40.0%
TCCTTGAGTTAGACGTATCT+CGG - chr5_1:26000217-26000236 None:intergenic 40.0%
GAATGCAGAACAATGAAGCA+CGG - chr5_1:26000244-26000263 None:intergenic 40.0%
CTTGGTTCAATCAGTTGAAC+CGG - chr5_1:26000483-26000502 None:intergenic 40.0%
! CAAGCAAATTGATTGGCTCT+TGG - chr5_1:26000501-26000520 None:intergenic 40.0%
! GAGCCAATCAATTTGCTTGT+TGG + chr5_1:26000502-26000521 Msa0725910:intron 40.0%
GCAATCTAGTTGAATCGGAT+CGG + chr5_1:26000543-26000562 Msa0725910:intron 40.0%
GTAGTTGAATCATGGTTCAC+CGG - chr5_1:26000589-26000608 None:intergenic 40.0%
TCAATCACACTTGAATCGGA+TGG - chr5_1:26000678-26000697 None:intergenic 40.0%
TTGCAGTAGTCAAACTGTTG+AGG + chr5_1:26000814-26000833 Msa0725910:intron 40.0%
TGCACAAATCCAGTTAGTGA+AGG - chr5_1:26000894-26000913 None:intergenic 40.0%
TTCACTAACTGGATTTGTGC+AGG + chr5_1:26000893-26000912 Msa0725910:intron 40.0%
TGTGGATAAATTGGGACAGA+TGG + chr5_1:26001137-26001156 Msa0725910:CDS 40.0%
GCACAAGATGAACCTGTAAA+TGG + chr5_1:26001691-26001710 Msa0725910:intron 40.0%
TGTAACTTCGCTCCATTTAC+AGG - chr5_1:26001706-26001725 None:intergenic 40.0%
! ATCTAGGATAAAGGCCTGTT+TGG - chr5_1:26001785-26001804 None:intergenic 40.0%
CTTACTCTGCCTACTTTGAT+AGG + chr5_1:26001839-26001858 Msa0725910:intron 40.0%
!!! CTGTTGGTGACTCATTTTGT+AGG - chr5_1:26002030-26002049 None:intergenic 40.0%
ACAGAGATTGAAGATGCTGT+TGG - chr5_1:26002046-26002065 None:intergenic 40.0%
AGCATCTTCAATCTCTGTCT+TGG + chr5_1:26002048-26002067 Msa0725910:CDS 40.0%
TCTTCAATCTCTGTCTTGGA+TGG + chr5_1:26002052-26002071 Msa0725910:CDS 40.0%
TCTCTGTCTTGGATGGTTAA+TGG + chr5_1:26002059-26002078 Msa0725910:CDS 40.0%
AGAAGCAAACTTGAGTCTTC+AGG - chr5_1:26002107-26002126 None:intergenic 40.0%
!! TATCCGGTGTCAATTTTGGT+TGG + chr5_1:26002305-26002324 Msa0725910:intron 40.0%
TAGCCAACCAAAATTGACAC+CGG - chr5_1:26002311-26002330 None:intergenic 40.0%
ACAACGATCAACTGATCTGA+GGG - chr5_1:26002382-26002401 None:intergenic 40.0%
GGAAGCTATGCTAGTTATCT+TGG + chr5_1:26003057-26003076 Msa0725910:CDS 40.0%
TATTCATCCCTGTAACTGGT+TGG - chr5_1:26003239-26003258 None:intergenic 40.0%
GGATTATTCATCCCTGTAAC+TGG - chr5_1:26003243-26003262 None:intergenic 40.0%
! GACTGATCAAACCCTTGTTT+AGG - chr5_1:26003315-26003334 None:intergenic 40.0%
CTGAATTAGAAGTCCAAGCA+TGG - chr5_1:26003347-26003366 None:intergenic 40.0%
CATGCTTGGACTTCTAATTC+AGG + chr5_1:26003345-26003364 Msa0725910:CDS 40.0%
ACTTGTGGAAAATTTCCCTG+AGG - chr5_1:26003408-26003427 None:intergenic 40.0%
GTGAATCCAAAATGCACTTG+TGG - chr5_1:26003423-26003442 None:intergenic 40.0%
AACCTTCATTATGCACAGGG+AGG + chr5_1:25998634-25998653 Msa0725910:CDS 45.0%
GGTGCACACTTTAATGCACT+TGG + chr5_1:25999000-25999019 Msa0725910:intron 45.0%
! AAGACGGTGTTTTTCCCACA+CGG - chr5_1:25999109-25999128 None:intergenic 45.0%
TGTCACGTTTCTCAAACGCA+CGG + chr5_1:25999465-25999484 Msa0725910:intron 45.0%
CTTCACAAGAAAGGGCAAAG+AGG + chr5_1:25999900-25999919 Msa0725910:CDS 45.0%
CAAGAAAGGGCAAAGAGGTA+CGG + chr5_1:25999905-25999924 Msa0725910:intron 45.0%
! AGTTAGACGTATCTCGGTGT+TGG - chr5_1:26000211-26000230 None:intergenic 45.0%
ACCGAGATACGTCTAACTCA+AGG + chr5_1:26000213-26000232 Msa0725910:intron 45.0%
TTTCGAAAACCGGACCGAAT+TGG + chr5_1:26000457-26000476 Msa0725910:intron 45.0%
ATCAGTTGAACCGGCCAATT+CGG - chr5_1:26000474-26000493 None:intergenic 45.0%
TCGAACCAGATCAGTTGAAC+CGG + chr5_1:26000567-26000586 Msa0725910:intron 45.0%
!! CACTGGTTCGATGTCATTTC+TGG + chr5_1:26000716-26000735 Msa0725910:intron 45.0%
GGTTCATCTTGTGCTCGATT+TGG - chr5_1:26001685-26001704 None:intergenic 45.0%
AAATCCAACCGTCCATTTGC+GGG - chr5_1:26002357-26002376 None:intergenic 45.0%
GAAATCCAACCGTCCATTTG+CGG - chr5_1:26002358-26002377 None:intergenic 45.0%
CACAACGATCAACTGATCTG+AGG - chr5_1:26002383-26002402 None:intergenic 45.0%
AGATCAGTTGATCGTTGTGC+CGG + chr5_1:26002384-26002403 Msa0725910:intron 45.0%
TGTCTACGTCAGTGCTTCAT+TGG + chr5_1:26002894-26002913 Msa0725910:intron 45.0%
GGCTCAACAAGCTCATCATT+TGG + chr5_1:26003036-26003055 Msa0725910:CDS 45.0%
GCAGCTTTCTTGACCAGAAT+TGG - chr5_1:26003106-26003125 None:intergenic 45.0%
ATGAAGCTCCAACCAGTTAC+AGG + chr5_1:26003228-26003247 Msa0725910:CDS 45.0%
TGAAGCTCCAACCAGTTACA+GGG + chr5_1:26003229-26003248 Msa0725910:CDS 45.0%
! GAAAGCGTGTGACAAGGCAA+AGG - chr5_1:25998929-25998948 None:intergenic 50.0%
! GGTGTTTTTCCCACACGGAT+TGG - chr5_1:25999104-25999123 None:intergenic 50.0%
CACAGATACCAGACACGACA+TGG + chr5_1:26000090-26000109 Msa0725910:intron 50.0%
GTCAAGATCCATGTCGTGTC+TGG - chr5_1:26000101-26000120 None:intergenic 50.0%
!! GTTCACCGGTTCAACTGATC+TGG - chr5_1:26000575-26000594 None:intergenic 50.0%
GGGCCGAAGTAGTTGAATCA+TGG - chr5_1:26000597-26000616 None:intergenic 50.0%
CCAGTGAGACTTGCGAATCA+TGG - chr5_1:26000702-26000721 None:intergenic 50.0%
CCATGATTCGCAAGTCTCAC+TGG + chr5_1:26000699-26000718 Msa0725910:intron 50.0%
!! CGGGCCGAGATTTTGTTGTA+TGG - chr5_1:26001732-26001751 None:intergenic 50.0%
!! GGGGCCATTTAAGCTCTGAA+TGG - chr5_1:26002336-26002355 None:intergenic 50.0%
AATCCAACCGTCCATTTGCG+GGG - chr5_1:26002356-26002375 None:intergenic 50.0%
GTACAGACACTCCTCGGATT+AGG + chr5_1:26002738-26002757 Msa0725910:intron 50.0%
! CATAGGTGTCGTGTCGATGT+TGG - chr5_1:26002800-26002819 None:intergenic 50.0%
CGCCTCCCTGTGCATAATGA+AGG - chr5_1:25998639-25998658 None:intergenic 55.0%
CACGTTTCTCAAACGCACGG+TGG + chr5_1:25999468-25999487 Msa0725910:intron 55.0%
!! TGTTGGTGTCGTGTCGGTGT+CGG + chr5_1:26000175-26000194 Msa0725910:intron 55.0%
GAAAACCGGACCGAATTGGC+CGG + chr5_1:26000461-26000480 Msa0725910:intron 55.0%
TTGAACCGGCCAATTCGGTC+CGG - chr5_1:26000469-26000488 None:intergenic 55.0%
GCTTAAATGGCCCCCGCAAA+TGG + chr5_1:26002342-26002361 Msa0725910:intron 55.0%
AAATGGCCCCCGCAAATGGA+CGG + chr5_1:26002346-26002365 Msa0725910:intron 55.0%
ATCCAACCGTCCATTTGCGG+GGG - chr5_1:26002355-26002374 None:intergenic 55.0%
ACTGGGACACACCTAATCCG+AGG - chr5_1:26002752-26002771 None:intergenic 55.0%
ACTGACGCGTGTTCGACACT+GGG - chr5_1:26002769-26002788 None:intergenic 55.0%
! GCACTGACGTAGACACGACA+CGG - chr5_1:26002889-26002908 None:intergenic 55.0%
! CATTATGCACAGGGAGGCGC+AGG + chr5_1:25998640-25998659 Msa0725910:CDS 60.0%
!! ACACGGATTGGCAACTCCGG+AGG - chr5_1:25999092-25999111 None:intergenic 60.0%
TCCGGAGTTGCCAATCCGTG+TGG + chr5_1:25999091-25999110 Msa0725910:intron 60.0%
!! CCCACACGGATTGGCAACTC+CGG - chr5_1:25999095-25999114 None:intergenic 60.0%
CCGGAGTTGCCAATCCGTGT+GGG + chr5_1:25999092-25999111 Msa0725910:intron 60.0%
CACTGACGCGTGTTCGACAC+TGG - chr5_1:26002770-26002789 None:intergenic 60.0%
CGGACACCGACACACTGACA+AGG - chr5_1:26002858-26002877 None:intergenic 60.0%
GACACGACACGGACACGACA+CGG - chr5_1:26002878-26002897 None:intergenic 60.0%
GGCCCCCGCAAATGGACGGT+TGG + chr5_1:26002350-26002369 Msa0725910:intron 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 25998477 26003446 25998477 ID=Msa0725910;Name=Msa0725910
chr5_1 mRNA 25998477 26003446 25998477 ID=Msa0725910-mRNA-1;Parent=Msa0725910;Name=Msa0725910-mRNA-1;_AED=0.09;_eAED=0.10;_QI=0|0|0|1|1|1|10|0|365
chr5_1 exon 25998477 25998661 25998477 ID=Msa0725910-mRNA-1:exon:9313;Parent=Msa0725910-mRNA-1
chr5_1 exon 25999862 25999921 25999862 ID=Msa0725910-mRNA-1:exon:9314;Parent=Msa0725910-mRNA-1
chr5_1 exon 26000020 26000038 26000020 ID=Msa0725910-mRNA-1:exon:9315;Parent=Msa0725910-mRNA-1
chr5_1 exon 26000345 26000409 26000345 ID=Msa0725910-mRNA-1:exon:9316;Parent=Msa0725910-mRNA-1
chr5_1 exon 26000820 26000835 26000820 ID=Msa0725910-mRNA-1:exon:9317;Parent=Msa0725910-mRNA-1
chr5_1 exon 26000915 26000976 26000915 ID=Msa0725910-mRNA-1:exon:9318;Parent=Msa0725910-mRNA-1
chr5_1 exon 26001107 26001203 26001107 ID=Msa0725910-mRNA-1:exon:9319;Parent=Msa0725910-mRNA-1
chr5_1 exon 26001861 26001908 26001861 ID=Msa0725910-mRNA-1:exon:9320;Parent=Msa0725910-mRNA-1
chr5_1 exon 26002008 26002132 26002008 ID=Msa0725910-mRNA-1:exon:9321;Parent=Msa0725910-mRNA-1
chr5_1 exon 26003026 26003446 26003026 ID=Msa0725910-mRNA-1:exon:9322;Parent=Msa0725910-mRNA-1
chr5_1 CDS 25998477 25998661 25998477 ID=Msa0725910-mRNA-1:cds;Parent=Msa0725910-mRNA-1
chr5_1 CDS 25999862 25999921 25999862 ID=Msa0725910-mRNA-1:cds;Parent=Msa0725910-mRNA-1
chr5_1 CDS 26000020 26000038 26000020 ID=Msa0725910-mRNA-1:cds;Parent=Msa0725910-mRNA-1
chr5_1 CDS 26000345 26000409 26000345 ID=Msa0725910-mRNA-1:cds;Parent=Msa0725910-mRNA-1
chr5_1 CDS 26000820 26000835 26000820 ID=Msa0725910-mRNA-1:cds;Parent=Msa0725910-mRNA-1
chr5_1 CDS 26000915 26000976 26000915 ID=Msa0725910-mRNA-1:cds;Parent=Msa0725910-mRNA-1
chr5_1 CDS 26001107 26001203 26001107 ID=Msa0725910-mRNA-1:cds;Parent=Msa0725910-mRNA-1
chr5_1 CDS 26001861 26001908 26001861 ID=Msa0725910-mRNA-1:cds;Parent=Msa0725910-mRNA-1
chr5_1 CDS 26002008 26002132 26002008 ID=Msa0725910-mRNA-1:cds;Parent=Msa0725910-mRNA-1
chr5_1 CDS 26003026 26003446 26003026 ID=Msa0725910-mRNA-1:cds;Parent=Msa0725910-mRNA-1
Gene Sequence

>Msa0725910

ATGGGGAGGGTAAAGCTAAAGATAAAAAGGTTGGAGAACACAAATGGACGCCAAGCAACCTATGCCAAAAGAAAGAATGGAATAATGAAGAAAGCAAGTGAATTATCTATATTGTGTGATATAGATATTATACTTCTTATGTTTTCTCCAGGTGGAAAACCTTCATTATGCACAGGGAGGCGCAGCAACTTTGAGGAGGTGATTGCAAAGTTTGCTCAACTAACTTCACAAGAAAGGGCAAAGAGGAAATTGGAGAGTCTTGAAGCACTAAAGAAAACATTTAAGAAGTTAGATCATGATGTGAATATACAAGACTTTTATGGTACAAGTAGTCAAACTGTTGAGGACTTGAGTGATCAAGCCAAGCTTTTACATAAGCGTATCTCCGAAATTCATGAGAGATTAAGCAATTGGTCTGACATTGAAAAAATTAGTAGTGTGGATAAATTGGGACAGATGGAAAATTCACTCAAGGAGTCTCTTAATCAAATTCAAACTCATAAGGAAAATGTACAAAAGCAACAACTGGCATCATTGCAATGCAATAATCAGTTCAATGAATTGCAGCTTCCCTACAAAATGAGTCACCAACAGCATCTTCAATCTCTGTCTTGGATGGTTAATGGTGACAGCCAGAATTTAGTGTTACCTGAAGACTCAAGTTTGCTTCTCCACAAAGATGTAGAAGGCTCAACAAGCTCATCATTTGGAAGCTATGCTAGTTATCTTGGCTCAAGCACAAAAACAAACATGTCCAATTCTGGTCAAGAAAGCTGCATCATTAATGACATGAGCAATACTACTACTGCTCCAGTTAGACTACAACAATTTAATGGACAAGTTCCATACATACCAAACAATTTCAACATTATGAATGATATGAAGCTCCAACCAGTTACAGGGATGAATAATCCACATGAAACTCAAGTAGATAATTATCATGTGAATGCAAATTTTGAAGCACCTAAACAAGGGTTTGATCAGTCTAATAATCACCATGCTTGGACTTCTAATTCAGGTCCTTGTGCTGTTAATATCTTTGATGAACATTTGTATCCTCAGGGAAATTTTCCACAAGTGCATTTTGGATTCACATAG
Protein sequence

>Msa0725910

MGRVKLKIKRLENTNGRQATYAKRKNGIMKKASELSILCDIDIILLMFSPGGKPSLCTGRRSNFEEVIAKFAQLTSQERAKRKLESLEALKKTFKKLDHDVNIQDFYGTSSQTVEDLSDQAKLLHKRISEIHERLSNWSDIEKISSVDKLGQMENSLKESLNQIQTHKENVQKQQLASLQCNNQFNELQLPYKMSHQQHLQSLSWMVNGDSQNLVLPEDSSLLLHKDVEGSTSSSFGSYASYLGSSTKTNMSNSGQESCIINDMSNTTTAPVRLQQFNGQVPYIPNNFNIMNDMKLQPVTGMNNPHETQVDNYHVNANFEAPKQGFDQSNNHHAWTSNSGPCAVNIFDEHLYPQGNFPQVHFGFT*