AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0727520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0727520 MtrunA17_Chr5g0419811 83.756 197 21 2 187 382 1 187 9.38e-111 322
Msa0727520 MtrunA17_Chr5g0419821 88.535 157 16 2 1 156 1 156 4.77e-90 268
Msa0727520 MtrunA17_Chr6g0481041 59.474 190 72 3 1 190 1 185 1.27e-66 224
Msa0727520 MtrunA17_Chr2g0322731 44.444 171 90 2 11 177 74 243 5.38e-42 156
Msa0727520 MtrunA17_Chr5g0410711 46.961 181 94 2 8 187 64 243 1.48e-41 155
Msa0727520 MtrunA17_Chr1g0203671 50.000 156 75 2 31 184 93 247 4.65e-41 154
Msa0727520 MtrunA17_Chr8g0350321 48.227 141 72 1 33 173 115 254 4.46e-40 151
Msa0727520 MtrunA17_Chr7g0246631 48.227 141 72 1 33 173 91 230 6.04e-40 150
Msa0727520 MtrunA17_Chr4g0024351 43.429 175 94 2 31 201 58 231 7.39e-40 150
Msa0727520 MtrunA17_Chr3g0109391 56.643 143 31 2 213 355 587 698 2.24e-38 146
Msa0727520 MtrunA17_Chr7g0224481 45.455 176 66 3 1 176 1 146 5.52e-37 137
Msa0727520 MtrunA17_Chr7g0224451 45.455 176 66 3 1 176 1 146 1.79e-36 137
Msa0727520 MtrunA17_Chr6g0472471 47.482 139 72 1 37 175 73 210 3.27e-35 137
Msa0727520 MtrunA17_Chr2g0331581 42.308 130 72 2 37 164 143 271 4.87e-26 110
Msa0727520 MtrunA17_Chr5g0433301 44.030 134 45 1 222 355 586 689 1.14e-23 103
Msa0727520 MtrunA17_Chr5g0394111 37.719 114 70 1 41 154 40 152 5.33e-19 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0727520 AT1G73360.1 56.329 158 68 1 33 190 32 188 3.97e-53 187
Msa0727520 AT1G17920.2 52.667 150 69 2 36 184 24 172 1.00e-46 169
Msa0727520 AT1G17920.1 52.667 150 69 2 36 184 24 172 1.00e-46 169
Msa0727520 AT4G04890.2 47.191 178 87 5 32 204 61 236 8.46e-41 153
Msa0727520 AT4G04890.1 47.191 178 87 5 32 204 61 236 8.46e-41 153
Msa0727520 AT4G00730.2 51.493 134 64 1 33 166 134 266 4.55e-40 150
Msa0727520 AT4G00730.1 51.493 134 64 1 33 166 134 266 8.93e-40 150
Msa0727520 AT4G21750.2 43.548 186 91 3 32 204 61 245 1.94e-39 149
Msa0727520 AT4G21750.1 43.548 186 91 3 32 204 61 245 1.94e-39 149
Msa0727520 AT4G21750.3 43.548 186 91 3 32 204 61 245 1.94e-39 149
Msa0727520 AT4G21750.4 43.548 186 91 3 32 204 61 245 1.94e-39 149
Msa0727520 AT1G05230.12 44.724 199 93 4 2 198 47 230 4.74e-39 147
Msa0727520 AT1G05230.3 44.724 199 93 4 2 198 47 230 8.59e-39 147
Msa0727520 AT1G05230.8 44.724 199 93 4 2 198 47 230 9.01e-39 147
Msa0727520 AT1G05230.4 44.724 199 93 4 2 198 47 230 9.01e-39 147
Msa0727520 AT1G05230.9 44.724 199 93 4 2 198 47 230 9.01e-39 147
Msa0727520 AT1G05230.10 44.724 199 93 4 2 198 47 230 9.01e-39 147
Msa0727520 AT1G05230.7 44.724 199 93 4 2 198 47 230 9.01e-39 147
Msa0727520 AT1G05230.11 44.724 199 93 4 2 198 47 230 9.01e-39 147
Msa0727520 AT1G05230.2 44.724 199 93 4 2 198 47 230 9.01e-39 147
Msa0727520 AT1G05230.6 44.724 199 93 4 2 198 47 230 9.01e-39 147
Msa0727520 AT1G05230.5 44.724 199 93 4 2 198 47 230 9.01e-39 147
Msa0727520 AT1G05230.1 44.724 199 93 4 2 198 47 230 9.01e-39 147
Msa0727520 AT3G61150.1 49.265 136 68 1 33 168 110 244 6.13e-38 145
Msa0727520 AT5G46880.3 46.259 147 76 2 32 178 110 253 1.22e-33 133
Msa0727520 AT5G46880.1 46.259 147 76 2 32 178 110 253 1.22e-33 133
Msa0727520 AT5G46880.2 46.259 147 76 2 32 178 110 253 1.22e-33 133
Msa0727520 AT5G52170.3 43.210 162 84 3 10 169 37 192 5.29e-33 130
Msa0727520 AT5G52170.1 43.210 162 84 3 10 169 37 192 5.29e-33 130
Msa0727520 AT5G52170.2 43.210 162 84 3 10 169 37 192 5.69e-33 130
Msa0727520 AT5G17320.1 47.020 151 78 2 27 176 20 169 4.99e-31 125
Msa0727520 AT1G34650.1 36.453 203 126 2 38 240 20 219 3.48e-30 122
Msa0727520 AT2G32370.1 41.714 175 95 4 27 197 62 233 5.77e-29 119
Msa0727520 AT3G03260.2 42.748 131 74 1 35 164 24 154 3.79e-27 113
Msa0727520 AT3G03260.1 42.748 131 74 1 35 164 24 154 4.19e-27 113
Msa0727520 AT1G79840.1 42.331 163 72 5 31 191 99 241 7.57e-26 110
Msa0727520 AT1G79840.5 42.331 163 72 5 31 191 99 241 8.14e-26 108
Msa0727520 AT1G79840.4 42.331 163 72 5 31 191 99 241 8.59e-26 109
Msa0727520 AT1G79840.3 42.331 163 72 5 31 191 41 183 8.64e-26 109
Msa0727520 AT1G79840.6 42.331 163 72 5 31 191 41 183 8.64e-26 109
Msa0727520 AT1G79840.2 42.331 163 72 5 31 191 128 270 1.19e-25 109
Msa0727520 AT4G17710.1 42.748 131 62 2 32 162 87 204 7.68e-24 103
Msa0727520 AT4G25530.1 33.058 121 79 1 36 154 40 160 6.01e-14 73.9

Find 108 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATCTTACCACATTGTTTC+TGG 0.133950 5_1:-30000506 Msa0727520:three_prime_UTR
ACAATGCAATTAGGGGAATT+TGG 0.144557 5_1:-30000768 Msa0727520:CDS
CGCCTGAGAAAATATGATTT+TGG 0.175687 5_1:+30000961 None:intergenic
ATGTCGCATGCGCAATATTC+TGG 0.202860 5_1:-30001026 Msa0727520:CDS
ACCAATTCAAATATCATCTT+TGG 0.219702 5_1:-30001273 Msa0727520:CDS
CCCAACTTTCTCTTATAAAT+AGG 0.235957 5_1:+30000455 None:intergenic
TTGGAAGGAGTATTACATTT+TGG 0.238603 5_1:+30000479 None:intergenic
GGTGCTTCGACAATGCAATT+AGG 0.245347 5_1:-30000777 Msa0727520:CDS
GTGCTTCGACAATGCAATTA+GGG 0.285038 5_1:-30000776 Msa0727520:CDS
GTTATGGTCAGATCAGATTT+TGG 0.293590 5_1:-30003189 Msa0727520:CDS
CAAGCGGGAGAAATACATTA+AGG 0.307728 5_1:-30000537 Msa0727520:three_prime_UTR
CAATGCAATTAGGGGAATTT+GGG 0.316222 5_1:-30000767 Msa0727520:CDS
CCGGGTTACACCACTTCATT+AGG 0.338198 5_1:-30000807 Msa0727520:CDS
AAAGAATTAGCTTTGAGTTA+TGG 0.345545 5_1:-30003205 Msa0727520:CDS
TCAACAAAAGTTGATGTATC+TGG 0.356195 5_1:-30000432 Msa0727520:three_prime_UTR
ACATCTTATCTTATCATTCT+CGG 0.356227 5_1:+30001994 None:intergenic
GTTGGAAACTCATTTGATAT+TGG 0.360541 5_1:-30001218 Msa0727520:CDS
GATCATTCTTGAATAGCATT+TGG 0.373233 5_1:-30000605 Msa0727520:CDS
TTAACGAAAGTTGATGAATC+TGG 0.373613 5_1:+30000396 None:intergenic
TTACTTCTTTGAATCTCGTT+AGG 0.387005 5_1:+30003429 None:intergenic
ACCTGAAGATATTCAAAAGA+TGG 0.405470 5_1:-30000565 Msa0727520:CDS
TCATCTTTGGATTCAACAAT+GGG 0.406650 5_1:-30001260 Msa0727520:CDS
GATTCAACAATGGGGAGTAT+TGG 0.411860 5_1:-30001251 Msa0727520:CDS
TCCAAAGATGATATTTGAAT+TGG 0.413568 5_1:+30001272 None:intergenic
AACAATTACCTCAACAATCA+TGG 0.416923 5_1:-30001094 Msa0727520:CDS
CCTATTTATAAGAGAAAGTT+GGG 0.422085 5_1:-30000455 Msa0727520:three_prime_UTR
TCATCGTTGCATTGCCCTAA+AGG 0.424737 5_1:-30001176 Msa0727520:CDS
AAAGATGATATTTGAATTGG+TGG 0.425517 5_1:+30001275 None:intergenic
TCATCTTTATTATTTATGTC+AGG 0.427766 5_1:-30000361 Msa0727520:three_prime_UTR
AAATAACAATCACCATTGAT+TGG 0.432230 5_1:+30001911 None:intergenic
TAGGGCAATGCAACGATGAA+TGG 0.441470 5_1:+30001180 None:intergenic
GCAATATTCTGGACAAGATT+CGG 0.442351 5_1:-30001015 Msa0727520:CDS
TCCTATTTATAAGAGAAAGT+TGG 0.442773 5_1:-30000456 Msa0727520:three_prime_UTR
GATATTTGAATTGGTGGAAC+TGG 0.454943 5_1:+30001281 None:intergenic
GAAAATTCAATCTGCCCCTC+TGG 0.456300 5_1:-30001944 Msa0727520:CDS
GTGTAACCCGGGATAGTGGT+GGG 0.458432 5_1:+30000819 None:intergenic
TGTAACCCGGGATAGTGGTG+GGG 0.459393 5_1:+30000820 None:intergenic
CTCTTATAAATAGGATTGGA+AGG 0.470484 5_1:+30000464 None:intergenic
CTTTCTCTTATAAATAGGAT+TGG 0.470767 5_1:+30000460 None:intergenic
GAAATGATAACCGTTCACTA+AGG 0.481739 5_1:-30002018 Msa0727520:CDS
GTAACCCGGGATAGTGGTGG+GGG 0.482359 5_1:+30000821 None:intergenic
GGACATCTAAATCAAAATGT+GGG 0.484157 5_1:+30000982 None:intergenic
AAGATCAGATGAATCGATGA+TGG 0.487644 5_1:+30000878 None:intergenic
GGGGAGTATTGGACAAGGAT+TGG 0.490348 5_1:-30001240 Msa0727520:CDS
CCTAATGAAGTGGTGTAACC+CGG 0.498260 5_1:+30000807 None:intergenic
ATCATCTTTGGATTCAACAA+TGG 0.498569 5_1:-30001261 Msa0727520:CDS
TGGACATCTAAATCAAAATG+TGG 0.502102 5_1:+30000981 None:intergenic
TCAAAAGATGGGATAACAAG+CGG 0.507610 5_1:-30000553 Msa0727520:exon
AAGCGGGAGAAATACATTAA+GGG 0.512222 5_1:-30000536 Msa0727520:three_prime_UTR
AGTATTGGACAAGGATTGGT+TGG 0.518348 5_1:-30001236 Msa0727520:CDS
GAGAAATACATTAAGGGCAT+TGG 0.518833 5_1:-30000530 Msa0727520:three_prime_UTR
ACCACTTCATTAGGTGAGAC+CGG 0.518859 5_1:-30000798 Msa0727520:CDS
CCTGAAGATATTCAAAAGAT+GGG 0.518915 5_1:-30000564 Msa0727520:CDS
AGATCAGATGAATCGATGGC+GGG 0.530532 5_1:+30000909 None:intergenic
GGTGGTTGTCCTCCAATCAA+TGG 0.533615 5_1:-30001923 Msa0727520:CDS
ATCAACAACAGACCACTACT+TGG 0.542104 5_1:+30001122 None:intergenic
AGATCAGATGAATCGATGAT+GGG 0.542926 5_1:+30000879 None:intergenic
GGTGTAACCCGGGATAGTGG+TGG 0.544160 5_1:+30000818 None:intergenic
ATGTGAGAACATATCTATGA+AGG 0.544559 5_1:-30001975 Msa0727520:CDS
GAGTATCAGAGTGGAGGAAG+TGG 0.546042 5_1:-30003501 Msa0727520:CDS
ATTAGGGGAATTTGGGTTCG+AGG 0.546765 5_1:-30000760 Msa0727520:CDS
AGTATGCAGGTCGATACCTT+TGG 0.549314 5_1:-30001056 Msa0727520:CDS
ATTTGAATTGGTGGAACTGG+TGG 0.550148 5_1:+30001284 None:intergenic
CAAAAGATGGGATAACAAGC+GGG 0.554197 5_1:-30000552 Msa0727520:exon
CATTCTCGGCCCTTAGTGAA+CGG 0.554659 5_1:+30002008 None:intergenic
ACCTGCATACTTTGATACCT+CGG 0.565962 5_1:+30001068 None:intergenic
AGATCAGATGAATCGATGGT+GGG 0.567478 5_1:+30000849 None:intergenic
TAATTGCATTGTCGAAGCAC+CGG 0.570679 5_1:+30000779 None:intergenic
TCCGAGGTATCAAAGTATGC+AGG 0.573808 5_1:-30001069 Msa0727520:CDS
TCAAGCTGATTCGGAGTGTG+AGG 0.574751 5_1:+30003387 None:intergenic
ATGCGACATTGCGTTGCCAA+AGG 0.575172 5_1:+30001040 None:intergenic
ATCAGATGAATCGATGGCGG+GGG 0.578383 5_1:+30000911 None:intergenic
AAATGATAACCGTTCACTAA+GGG 0.581718 5_1:-30002017 Msa0727520:CDS
GATCAGATGAATCGATGGCG+GGG 0.582142 5_1:+30000910 None:intergenic
AGTGGTGTAACCCGGGATAG+TGG 0.583646 5_1:+30000815 None:intergenic
AGCATTATATCTGAATACAC+TGG 0.584252 5_1:-30001326 Msa0727520:CDS
GACATCTAAATCAAAATGTG+GGG 0.585558 5_1:+30000983 None:intergenic
TTAGGGGAATTTGGGTTCGA+GGG 0.586575 5_1:-30000759 Msa0727520:CDS
AATTCAATCTGCCCCTCTGG+TGG 0.586869 5_1:-30001941 Msa0727520:CDS
TAACAATCACCATTGATTGG+AGG 0.588494 5_1:+30001914 None:intergenic
TCGAAGATCAGATGAATCGA+TGG 0.588550 5_1:+30000905 None:intergenic
GCTGATTCGGAGTGTGAGGA+CGG 0.593800 5_1:+30003391 None:intergenic
ACCGGTCTCACCTAATGAAG+TGG 0.597496 5_1:+30000797 None:intergenic
AAGATCAGATGAATCGATGG+TGG 0.602370 5_1:+30000848 None:intergenic
GAAACTGGTACTTACTCCCA+CGG 0.602394 5_1:+30000726 None:intergenic
AAATGCTCTACTCAAAGAAG+AGG 0.606481 5_1:-30001864 Msa0727520:intron
GATCAGATGAATCGATGGTG+GGG 0.608527 5_1:+30000850 None:intergenic
GACATGGAGTATCAGAGTGG+AGG 0.611512 5_1:-30003507 Msa0727520:CDS
CTCGGAGTCCATGATTGTTG+AGG 0.616580 5_1:+30001086 None:intergenic
GAACAAAAGGGTTCAGACGA+AGG 0.619839 5_1:-30003161 Msa0727520:intron
CTATCAAGTTTACCAAGTAG+TGG 0.620797 5_1:-30001134 Msa0727520:CDS
TAACCCGGGATAGTGGTGGG+GGG 0.624406 5_1:+30000822 None:intergenic
TGAATCTCGTTAGGAGTGTG+AGG 0.625793 5_1:+30003438 None:intergenic
CTAATGAAGTGGTGTAACCC+GGG 0.626455 5_1:+30000808 None:intergenic
TAGAAGATCAGATGAATCGA+TGG 0.635082 5_1:+30000845 None:intergenic
ATTGGAGGACAACCACCAGA+GGG 0.645157 5_1:+30001929 None:intergenic
GATCAGATGAATCGATGATG+GGG 0.647479 5_1:+30000880 None:intergenic
TTGGTTGTCCAGAAACAATG+TGG 0.649003 5_1:+30000498 None:intergenic
TCAGATGAATCGATGGCGGG+GGG 0.666320 5_1:+30000912 None:intergenic
AAGATCAGATGAATCGATGG+CGG 0.668416 5_1:+30000908 None:intergenic
TGCTTCGACAATGCAATTAG+GGG 0.669628 5_1:-30000775 Msa0727520:CDS
GATTGGAGGACAACCACCAG+AGG 0.675241 5_1:+30001928 None:intergenic
CTCAACAATCATGGACTCCG+AGG 0.676275 5_1:-30001085 Msa0727520:CDS
ACATCTAAATCAAAATGTGG+GGG 0.693255 5_1:+30000984 None:intergenic
AGAGACATGGAGTATCAGAG+TGG 0.696792 5_1:-30003510 None:intergenic
ACAATGGGGAGTATTGGACA+AGG 0.710979 5_1:-30001245 Msa0727520:CDS
CATCTTTGGATTCAACAATG+GGG 0.731611 5_1:-30001259 Msa0727520:CDS
TTGGAGGACAACCACCAGAG+GGG 0.763412 5_1:+30001930 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAAAATAAAATAAA+AGG + chr5_1:30000489-30000508 None:intergenic 0.0%
!!! TTTATTTTATTTTATTTTTT+AGG - chr5_1:30000494-30000513 Msa0727520:three_prime_UTR 0.0%
!!! TTATTTTATTTTATTTTTTA+GGG - chr5_1:30000495-30000514 Msa0727520:three_prime_UTR 0.0%
!! ATAATAATAATAATAATAAT+AGG + chr5_1:30001566-30001585 None:intergenic 0.0%
!!! TTTCAAATCTTTTTTTTATA+TGG - chr5_1:30000463-30000482 Msa0727520:three_prime_UTR 10.0%
!! AAAAAATTAAAAACACAATT+AGG + chr5_1:30000795-30000814 None:intergenic 10.0%
!!! TATTTTTAAATGATTCTTTA+TGG - chr5_1:30001699-30001718 Msa0727520:intron 10.0%
!!! TTATTCATAATTTAAAATTG+TGG - chr5_1:30002184-30002203 Msa0727520:intron 10.0%
!!! CTTGTTTTATATTTTTTATT+TGG - chr5_1:30002356-30002375 Msa0727520:intron 10.0%
!!! ATATACATGATATTGTTATT+AGG - chr5_1:30000824-30000843 Msa0727520:CDS 15.0%
!!! TATACATGATATTGTTATTA+GGG - chr5_1:30000825-30000844 Msa0727520:CDS 15.0%
!! TTCCTTAATAAATATCATTA+AGG + chr5_1:30000863-30000882 None:intergenic 15.0%
!!! ACAAAATTTATTTGATTTCT+TGG + chr5_1:30000974-30000993 None:intergenic 15.0%
!! GATTTCTTGAAATAATAAAT+GGG + chr5_1:30001038-30001057 None:intergenic 15.0%
!! TGATTTCTTGAAATAATAAA+TGG + chr5_1:30001039-30001058 None:intergenic 15.0%
!! GATTTCTTGAAATAATAAAT+GGG + chr5_1:30001038-30001057 None:intergenic 15.0%
!! TGATTTCTTGAAATAATAAA+TGG + chr5_1:30001039-30001058 None:intergenic 15.0%
!! GATTTCTTGAAATAATAAAT+GGG + chr5_1:30001038-30001057 None:intergenic 15.0%
!! TGATTTCTTGAAATAATAAA+TGG + chr5_1:30001039-30001058 None:intergenic 15.0%
!!! GTTTATCCTTTAAAAAAAAT+AGG - chr5_1:30001329-30001348 Msa0727520:CDS 15.0%
!!! AACTTACCTATTTTTTTTAA+AGG + chr5_1:30001338-30001357 None:intergenic 15.0%
!! AGAAATATTGTATTGAAAAA+TGG + chr5_1:30001495-30001514 None:intergenic 15.0%
!!! ATTGTTATTTTGATAGAAAA+AGG - chr5_1:30001875-30001894 Msa0727520:CDS 15.0%
!!! ACAAATATTTTGATGAATTT+TGG + chr5_1:30002117-30002136 None:intergenic 15.0%
!! AATTCATCAAAATATTTGTA+AGG - chr5_1:30002118-30002137 Msa0727520:intron 15.0%
!! ATTCATCAAAATATTTGTAA+GGG - chr5_1:30002119-30002138 Msa0727520:intron 15.0%
!!! GTAAATATGTTTTTAAATTG+CGG + chr5_1:30002337-30002356 None:intergenic 15.0%
!! ATAATAAAGATGACAAAAAT+TGG + chr5_1:30003405-30003424 None:intergenic 15.0%
!!! TTTATTTTTTAGGGTGTTTA+AGG - chr5_1:30000504-30000523 Msa0727520:three_prime_UTR 20.0%
!! GAATTATAAAGTTTAACTGT+CGG - chr5_1:30000728-30000747 Msa0727520:intron 20.0%
!! TGCCTTAATGATATTTATTA+AGG - chr5_1:30000858-30000877 Msa0727520:CDS 20.0%
!! TTAAGGAAATCAAAAGTAAT+AGG - chr5_1:30000875-30000894 Msa0727520:CDS 20.0%
!! TTGAAATAATAAATGGGTAA+GGG + chr5_1:30001032-30001051 None:intergenic 20.0%
!! TTGAAATAATAAATGGGTAA+GGG + chr5_1:30001032-30001051 None:intergenic 20.0%
!! TTGAAATAATAAATGGGTAA+GGG + chr5_1:30001032-30001051 None:intergenic 20.0%
!! TTCAATGCAACATTAATTAT+TGG + chr5_1:30001374-30001393 None:intergenic 20.0%
!! AAACAAAATTATTCTCCAAA+AGG - chr5_1:30001410-30001429 Msa0727520:intron 20.0%
!! AACAAAATTATTCTCCAAAA+GGG - chr5_1:30001411-30001430 Msa0727520:intron 20.0%
!!! TCTTTATGACATTGATTAAA+AGG + chr5_1:30001526-30001545 None:intergenic 20.0%
!! TCATCTTTATTATTTATGTC+AGG - chr5_1:30003412-30003431 Msa0727520:CDS 20.0%
!!! TTTTGTTTTTCATCAGGTTT+TGG + chr5_1:30000535-30000554 None:intergenic 25.0%
!!! AAAGAATTAGCTTTGAGTTA+TGG - chr5_1:30000568-30000587 Msa0727520:CDS 25.0%
!!! ATTTTGGTTTCAGAACAAAA+GGG - chr5_1:30000600-30000619 Msa0727520:CDS 25.0%
!! ATGTTGTTCTTGTGTTTTAT+TGG - chr5_1:30000706-30000725 Msa0727520:intron 25.0%
! AAACGACAAGTATTATGAAA+CGG + chr5_1:30000933-30000952 None:intergenic 25.0%
! CTTGAAATAATAAATGGGTA+AGG + chr5_1:30001033-30001052 None:intergenic 25.0%
! CTTGAAATAATAAATGGGTA+AGG + chr5_1:30001033-30001052 None:intergenic 25.0%
! CTTGAAATAATAAATGGGTA+AGG + chr5_1:30001033-30001052 None:intergenic 25.0%
! ACATCTTATCTTATCATTCT+CGG + chr5_1:30001782-30001801 None:intergenic 25.0%
! AAATAACAATCACCATTGAT+TGG + chr5_1:30001865-30001884 None:intergenic 25.0%
! GTCTCTGTAAAAGAAAAATA+AGG - chr5_1:30002090-30002109 Msa0727520:intron 25.0%
! AAAGATGATATTTGAATTGG+TGG + chr5_1:30002501-30002520 None:intergenic 25.0%
! ACCAATTCAAATATCATCTT+TGG - chr5_1:30002500-30002519 Msa0727520:intron 25.0%
! TCCAAAGATGATATTTGAAT+TGG + chr5_1:30002504-30002523 None:intergenic 25.0%
!! AGAGTTTTTCTTTTCCTTTA+GGG + chr5_1:30002614-30002633 None:intergenic 25.0%
!! AAGAGTTTTTCTTTTCCTTT+AGG + chr5_1:30002615-30002634 None:intergenic 25.0%
! CTTTCTCTTATAAATAGGAT+TGG + chr5_1:30003316-30003335 None:intergenic 25.0%
! TCCTATTTATAAGAGAAAGT+TGG - chr5_1:30003317-30003336 Msa0727520:intron 25.0%
! CCTATTTATAAGAGAAAGTT+GGG - chr5_1:30003318-30003337 Msa0727520:intron 25.0%
TTACTTCTTTGAATCTCGTT+AGG + chr5_1:30000347-30000366 None:intergenic 30.0%
!!! TGCAACTTTTGTTTTTCATC+AGG + chr5_1:30000541-30000560 None:intergenic 30.0%
!!! GATTTTGGTTTCAGAACAAA+AGG - chr5_1:30000599-30000618 Msa0727520:CDS 30.0%
GCAAAAACATACACACATAA+AGG + chr5_1:30000773-30000792 None:intergenic 30.0%
CAAAAGTAATAGGATTGCAT+TGG - chr5_1:30000885-30000904 Msa0727520:CDS 30.0%
!!! CATAATACTTGTCGTTTTTG+AGG - chr5_1:30000936-30000955 Msa0727520:CDS 30.0%
TCTCTCTCTCTACAATAAAT+AGG - chr5_1:30001303-30001322 Msa0727520:CDS 30.0%
!!! GGTTTTGATTTGAACAAAAC+AGG - chr5_1:30001720-30001739 Msa0727520:intron 30.0%
AAATGATAACCGTTCACTAA+GGG - chr5_1:30001756-30001775 Msa0727520:intron 30.0%
ATGTGAGAACATATCTATGA+AGG - chr5_1:30001798-30001817 Msa0727520:intron 30.0%
ATTTACACGCACAATTTCAA+GGG - chr5_1:30001989-30002008 Msa0727520:CDS 30.0%
! TCTTTTACAGAGACTAAAGA+GGG + chr5_1:30002084-30002103 None:intergenic 30.0%
! TTCTTTTACAGAGACTAAAG+AGG + chr5_1:30002085-30002104 None:intergenic 30.0%
! AATTTAAAATTGTGGCTGCA+TGG - chr5_1:30002192-30002211 Msa0727520:intron 30.0%
! ATTTAAAATTGTGGCTGCAT+GGG - chr5_1:30002193-30002212 Msa0727520:intron 30.0%
!! AATATAAAGATTTTGACCCG+AGG + chr5_1:30002291-30002310 None:intergenic 30.0%
AGCATTATATCTGAATACAC+TGG - chr5_1:30002447-30002466 Msa0727520:intron 30.0%
ATCATCTTTGGATTCAACAA+TGG - chr5_1:30002512-30002531 Msa0727520:intron 30.0%
TCATCTTTGGATTCAACAAT+GGG - chr5_1:30002513-30002532 Msa0727520:intron 30.0%
GTTGGAAACTCATTTGATAT+TGG - chr5_1:30002555-30002574 Msa0727520:intron 30.0%
AACAATTACCTCAACAATCA+TGG - chr5_1:30002679-30002698 Msa0727520:intron 30.0%
ACATCTAAATCAAAATGTGG+GGG + chr5_1:30002792-30002811 None:intergenic 30.0%
GACATCTAAATCAAAATGTG+GGG + chr5_1:30002793-30002812 None:intergenic 30.0%
GGACATCTAAATCAAAATGT+GGG + chr5_1:30002794-30002813 None:intergenic 30.0%
TGGACATCTAAATCAAAATG+TGG + chr5_1:30002795-30002814 None:intergenic 30.0%
! GTCCAAAATCATATTTTCTC+AGG - chr5_1:30002810-30002829 Msa0727520:intron 30.0%
!! CTTGATGATGTAATTGAAAC+TGG + chr5_1:30003065-30003084 None:intergenic 30.0%
GATCATTCTTGAATAGCATT+TGG - chr5_1:30003168-30003187 Msa0727520:CDS 30.0%
ACCTGAAGATATTCAAAAGA+TGG - chr5_1:30003208-30003227 Msa0727520:CDS 30.0%
CCTGAAGATATTCAAAAGAT+GGG - chr5_1:30003209-30003228 Msa0727520:CDS 30.0%
!!! TTGGAAGGAGTATTACATTT+TGG + chr5_1:30003297-30003316 None:intergenic 30.0%
CTCTTATAAATAGGATTGGA+AGG + chr5_1:30003312-30003331 None:intergenic 30.0%
CCCAACTTTCTCTTATAAAT+AGG + chr5_1:30003321-30003340 None:intergenic 30.0%
!! TCAACAAAAGTTGATGTATC+TGG - chr5_1:30003341-30003360 Msa0727520:intron 30.0%
!! TTAACGAAAGTTGATGAATC+TGG + chr5_1:30003380-30003399 None:intergenic 30.0%
!! TCAAGCTTTTCAAGCTGATT+CGG + chr5_1:30000398-30000417 None:intergenic 35.0%
! GTTATGGTCAGATCAGATTT+TGG - chr5_1:30000584-30000603 Msa0727520:CDS 35.0%
GACAAGTATTATGAAACGGA+GGG + chr5_1:30000929-30000948 None:intergenic 35.0%
! TTCACAATACATGTCCCTTT+TGG + chr5_1:30001428-30001447 None:intergenic 35.0%
AAGGGACATGTATTGTGAAA+CGG - chr5_1:30001429-30001448 Msa0727520:intron 35.0%
GAAATGATAACCGTTCACTA+AGG - chr5_1:30001755-30001774 Msa0727520:intron 35.0%
! TAACAATCACCATTGATTGG+AGG + chr5_1:30001862-30001881 None:intergenic 35.0%
AAATGCTCTACTCAAAGAAG+AGG - chr5_1:30001909-30001928 Msa0727520:CDS 35.0%
!!! CGCATGTTATTTTGCATTAG+CGG + chr5_1:30001958-30001977 None:intergenic 35.0%
CATTTACACGCACAATTTCA+AGG - chr5_1:30001988-30002007 Msa0727520:CDS 35.0%
! TTTAAAATTGTGGCTGCATG+GGG - chr5_1:30002194-30002213 Msa0727520:intron 35.0%
GGGTGTGAACATTTATATTG+TGG - chr5_1:30002215-30002234 Msa0727520:intron 35.0%
! TACACTGGAAGATCAGTTTT+AGG - chr5_1:30002462-30002481 Msa0727520:intron 35.0%
! ACACTGGAAGATCAGTTTTA+GGG - chr5_1:30002463-30002482 Msa0727520:intron 35.0%
GATATTTGAATTGGTGGAAC+TGG + chr5_1:30002495-30002514 None:intergenic 35.0%
CATCTTTGGATTCAACAATG+GGG - chr5_1:30002514-30002533 Msa0727520:intron 35.0%
CTATCAAGTTTACCAAGTAG+TGG - chr5_1:30002639-30002658 Msa0727520:intron 35.0%
GCAATATTCTGGACAAGATT+CGG - chr5_1:30002758-30002777 Msa0727520:intron 35.0%
! CGCCTGAGAAAATATGATTT+TGG + chr5_1:30002815-30002834 None:intergenic 35.0%
AGATCAGATGAATCGATGAT+GGG + chr5_1:30002897-30002916 None:intergenic 35.0%
AAGATCAGATGAATCGATGA+TGG + chr5_1:30002898-30002917 None:intergenic 35.0%
TAGAAGATCAGATGAATCGA+TGG + chr5_1:30002841-30002860 None:intergenic 35.0%
ACAATGCAATTAGGGGAATT+TGG - chr5_1:30003005-30003024 Msa0727520:intron 35.0%
CAATGCAATTAGGGGAATTT+GGG - chr5_1:30003006-30003025 Msa0727520:intron 35.0%
! CCCATCTTTTGAATATCTTC+AGG + chr5_1:30003212-30003231 None:intergenic 35.0%
TCAAAAGATGGGATAACAAG+CGG - chr5_1:30003220-30003239 Msa0727520:CDS 35.0%
AAGCGGGAGAAATACATTAA+GGG - chr5_1:30003237-30003256 Msa0727520:CDS 35.0%
! GAGAAATACATTAAGGGCAT+TGG - chr5_1:30003243-30003262 Msa0727520:CDS 35.0%
CAATCTTACCACATTGTTTC+TGG - chr5_1:30003267-30003286 Msa0727520:intron 35.0%
CGACAAGTATTATGAAACGG+AGG + chr5_1:30000930-30000949 None:intergenic 40.0%
! GGGGCAGATTGAATTTTCTA+GGG + chr5_1:30001827-30001846 None:intergenic 40.0%
! AGGGGCAGATTGAATTTTCT+AGG + chr5_1:30001828-30001847 None:intergenic 40.0%
TTAAAATTGTGGCTGCATGG+GGG - chr5_1:30002195-30002214 Msa0727520:intron 40.0%
ATTTGAATTGGTGGAACTGG+TGG + chr5_1:30002492-30002511 None:intergenic 40.0%
! GATTCAACAATGGGGAGTAT+TGG - chr5_1:30002522-30002541 Msa0727520:intron 40.0%
! AGTATTGGACAAGGATTGGT+TGG - chr5_1:30002537-30002556 Msa0727520:intron 40.0%
ATCAACAACAGACCACTACT+TGG + chr5_1:30002654-30002673 None:intergenic 40.0%
ACCTGCATACTTTGATACCT+CGG + chr5_1:30002708-30002727 None:intergenic 40.0%
AAGATCAGATGAATCGATGG+CGG + chr5_1:30002868-30002887 None:intergenic 40.0%
TCGAAGATCAGATGAATCGA+TGG + chr5_1:30002871-30002890 None:intergenic 40.0%
GATCAGATGAATCGATGATG+GGG + chr5_1:30002896-30002915 None:intergenic 40.0%
AGATCAGATGAATCGATGGT+GGG + chr5_1:30002927-30002946 None:intergenic 40.0%
AAGATCAGATGAATCGATGG+TGG + chr5_1:30002868-30002887 None:intergenic 40.0%
TAATTGCATTGTCGAAGCAC+CGG + chr5_1:30002997-30003016 None:intergenic 40.0%
GTGCTTCGACAATGCAATTA+GGG - chr5_1:30002997-30003016 Msa0727520:intron 40.0%
TGCTTCGACAATGCAATTAG+GGG - chr5_1:30002998-30003017 Msa0727520:intron 40.0%
CAAAAGATGGGATAACAAGC+GGG - chr5_1:30003221-30003240 Msa0727520:CDS 40.0%
CAAGCGGGAGAAATACATTA+AGG - chr5_1:30003236-30003255 Msa0727520:CDS 40.0%
TTGGTTGTCCAGAAACAATG+TGG + chr5_1:30003278-30003297 None:intergenic 40.0%
! TGAATCTCGTTAGGAGTGTG+AGG + chr5_1:30000338-30000357 None:intergenic 45.0%
GAACAAAAGGGTTCAGACGA+AGG - chr5_1:30000612-30000631 Msa0727520:intron 45.0%
GGACATGTATTGTGAAACGG+AGG - chr5_1:30001432-30001451 Msa0727520:intron 45.0%
GAAAATTCAATCTGCCCCTC+TGG - chr5_1:30001829-30001848 Msa0727520:intron 45.0%
TATTGCGACCACAACTGCAA+TGG - chr5_1:30002307-30002326 Msa0727520:intron 45.0%
ATTGCGACCACAACTGCAAT+GGG - chr5_1:30002308-30002327 Msa0727520:intron 45.0%
!! ACAATGGGGAGTATTGGACA+AGG - chr5_1:30002528-30002547 Msa0727520:intron 45.0%
TAGGGCAATGCAACGATGAA+TGG + chr5_1:30002596-30002615 None:intergenic 45.0%
TCATCGTTGCATTGCCCTAA+AGG - chr5_1:30002597-30002616 Msa0727520:intron 45.0%
! TCCGAGGTATCAAAGTATGC+AGG - chr5_1:30002704-30002723 Msa0727520:intron 45.0%
AGTATGCAGGTCGATACCTT+TGG - chr5_1:30002717-30002736 Msa0727520:intron 45.0%
ATGTCGCATGCGCAATATTC+TGG - chr5_1:30002747-30002766 Msa0727520:intron 45.0%
AGATCAGATGAATCGATGGC+GGG + chr5_1:30002867-30002886 None:intergenic 45.0%
GATCAGATGAATCGATGGTG+GGG + chr5_1:30002926-30002945 None:intergenic 45.0%
CTAATGAAGTGGTGTAACCC+GGG + chr5_1:30002968-30002987 None:intergenic 45.0%
CCTAATGAAGTGGTGTAACC+CGG + chr5_1:30002969-30002988 None:intergenic 45.0%
ACCACTTCATTAGGTGAGAC+CGG - chr5_1:30002975-30002994 Msa0727520:intron 45.0%
GGTGCTTCGACAATGCAATT+AGG - chr5_1:30002996-30003015 Msa0727520:intron 45.0%
! ATTAGGGGAATTTGGGTTCG+AGG - chr5_1:30003013-30003032 Msa0727520:intron 45.0%
! TTAGGGGAATTTGGGTTCGA+GGG - chr5_1:30003014-30003033 Msa0727520:intron 45.0%
GAAACTGGTACTTACTCCCA+CGG + chr5_1:30003050-30003069 None:intergenic 45.0%
!!! TTTTTAATATACTTAAAAAT+TGG + chr5_1:30002045-30002064 None:intergenic 5.0%
!! TCATATAATTTAAAAATAAA+AGG + chr5_1:30003122-30003141 None:intergenic 5.0%
GAGTATCAGAGTGGAGGAAG+TGG - chr5_1:30000272-30000291 Msa0727520:three_prime_UTR 50.0%
!! TCAAGCTGATTCGGAGTGTG+AGG + chr5_1:30000389-30000408 None:intergenic 50.0%
CATTCTCGGCCCTTAGTGAA+CGG + chr5_1:30001768-30001787 None:intergenic 50.0%
AATTCAATCTGCCCCTCTGG+TGG - chr5_1:30001832-30001851 Msa0727520:intron 50.0%
ATTGGAGGACAACCACCAGA+GGG + chr5_1:30001847-30001866 None:intergenic 50.0%
GGTGGTTGTCCTCCAATCAA+TGG - chr5_1:30001850-30001869 Msa0727520:intron 50.0%
! GAGTGTCGTTGCGAAAACCT+CGG - chr5_1:30002271-30002290 Msa0727520:intron 50.0%
! AGTGTCGTTGCGAAAACCTC+GGG - chr5_1:30002272-30002291 Msa0727520:intron 50.0%
!! GGGGAGTATTGGACAAGGAT+TGG - chr5_1:30002533-30002552 Msa0727520:intron 50.0%
CTCGGAGTCCATGATTGTTG+AGG + chr5_1:30002690-30002709 None:intergenic 50.0%
CTCAACAATCATGGACTCCG+AGG - chr5_1:30002688-30002707 Msa0727520:intron 50.0%
ATGCGACATTGCGTTGCCAA+AGG + chr5_1:30002736-30002755 None:intergenic 50.0%
! ATCAGATGAATCGATGGCGG+GGG + chr5_1:30002865-30002884 None:intergenic 50.0%
GATCAGATGAATCGATGGCG+GGG + chr5_1:30002866-30002885 None:intergenic 50.0%
CCGGGTTACACCACTTCATT+AGG - chr5_1:30002966-30002985 Msa0727520:intron 50.0%
ACCGGTCTCACCTAATGAAG+TGG + chr5_1:30002979-30002998 None:intergenic 50.0%
!! TCGAGGGTTTTGATGATCCG+TGG - chr5_1:30003030-30003049 Msa0727520:intron 50.0%
!! CGAGGGTTTTGATGATCCGT+GGG - chr5_1:30003031-30003050 Msa0727520:intron 50.0%
!! GCTGATTCGGAGTGTGAGGA+CGG + chr5_1:30000385-30000404 None:intergenic 55.0%
TTGGAGGACAACCACCAGAG+GGG + chr5_1:30001846-30001865 None:intergenic 55.0%
GATTGGAGGACAACCACCAG+AGG + chr5_1:30001848-30001867 None:intergenic 55.0%
!! TCAGATGAATCGATGGCGGG+GGG + chr5_1:30002864-30002883 None:intergenic 55.0%
! TGTAACCCGGGATAGTGGTG+GGG + chr5_1:30002956-30002975 None:intergenic 55.0%
! GTGTAACCCGGGATAGTGGT+GGG + chr5_1:30002957-30002976 None:intergenic 55.0%
AGTGGTGTAACCCGGGATAG+TGG + chr5_1:30002961-30002980 None:intergenic 55.0%
TCTACCCCCCACCACTATCC+CGG - chr5_1:30002947-30002966 Msa0727520:intron 60.0%
! TAACCCGGGATAGTGGTGGG+GGG + chr5_1:30002954-30002973 None:intergenic 60.0%
! GTAACCCGGGATAGTGGTGG+GGG + chr5_1:30002955-30002974 None:intergenic 60.0%
! GGTGTAACCCGGGATAGTGG+TGG + chr5_1:30002958-30002977 None:intergenic 60.0%
GCGGTCGCCCATTGCAGTTG+TGG + chr5_1:30002318-30002337 None:intergenic 65.0%
CTACCCCCCACCACTATCCC+GGG - chr5_1:30002948-30002967 Msa0727520:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 30000269 30003526 30000269 ID=Msa0727520;Name=Msa0727520
chr5_1 mRNA 30000269 30003526 30000269 ID=Msa0727520-mRNA-1;Parent=Msa0727520;Name=Msa0727520-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0.5|0.2|1|1|1|5|291|382
chr5_1 exon 30000269 30000626 30000269 ID=Msa0727520-mRNA-1:exon:10197;Parent=Msa0727520-mRNA-1
chr5_1 exon 30000741 30001363 30000741 ID=Msa0727520-mRNA-1:exon:10196;Parent=Msa0727520-mRNA-1
chr5_1 exon 30001865 30002053 30001865 ID=Msa0727520-mRNA-1:exon:10195;Parent=Msa0727520-mRNA-1
chr5_1 exon 30003162 30003279 30003162 ID=Msa0727520-mRNA-1:exon:10194;Parent=Msa0727520-mRNA-1
chr5_1 exon 30003375 30003526 30003375 ID=Msa0727520-mRNA-1:exon:10193;Parent=Msa0727520-mRNA-1
chr5_1 CDS 30003375 30003526 30003375 ID=Msa0727520-mRNA-1:cds;Parent=Msa0727520-mRNA-1
chr5_1 CDS 30003162 30003279 30003162 ID=Msa0727520-mRNA-1:cds;Parent=Msa0727520-mRNA-1
chr5_1 CDS 30001865 30002053 30001865 ID=Msa0727520-mRNA-1:cds;Parent=Msa0727520-mRNA-1
chr5_1 CDS 30000741 30001363 30000741 ID=Msa0727520-mRNA-1:cds;Parent=Msa0727520-mRNA-1
chr5_1 CDS 30000560 30000626 30000560 ID=Msa0727520-mRNA-1:cds;Parent=Msa0727520-mRNA-1
chr5_1 three_prime_UTR 30000269 30000559 30000269 ID=Msa0727520-mRNA-1:three_prime_utr;Parent=Msa0727520-mRNA-1
Gene Sequence

>Msa0727520

ATGGAGTATCAGAGTGGAGGAAGTGGTTCTTTTGATTCTCAAAGTAAGAAAAAACGTTATCGTCGTCCTCACACTCCTAACGAGATTCAAAGAAGTAAGAAGAAACGTTATCGCCGTCCTCACACTCCGAATCAGCTTGAAAAGCTTGAAGAGGTGTTTAAGGAGTGTCCAAAACCTGATGAAAAACAAAAGTTGCAATTAAGCAAAGAATTAGCTTTGAGTTATGGTCAGATCAGATTTTGGTTTCAGAACAAAAGGGTTCAGACGAAGGCAAAACTTGAAAGAAATGATAACCGTTCACTAAGGGCCGAGAATGATAAGATAAGATGTGAGAACATATCTATGAAGGAAGCCCTAGAAAATTCAATCTGCCCCTCTGGTGGTTGTCCTCCAATCAATGGTGATTGTTATTTTGATAGAAAAAGGTTGAGACTTGAAAATGCTCTACTCAAAGAAGAGCTTTATAAAGAATCTAGCATTATATCTGAATACACTGGAAGATCAGTTTTAGGGTTTCCACCAGTTCCACCAATTCAAATATCATCTTTGGATTCAACAATGGGGAGTATTGGACAAGGATTGGTTGGAAACTCATTTGATATTGGTGAAATTCATAACAACCATTCATCGTTGCATTGCCCTAAAGGAAAAGAAAAACTCTTCAATCTATCAAGTTTACCAAGTAGTGGTCTGTTGTTGATGAATGAACAATTACCTCAACAATCATGGACTCCGAGGTATCAAAGTATGCAGGTCGATACCTTTGGCAACGCAATGTCGCATGCGCAATATTCTGGACAAGATTCGGCAATTGTGCCCCCACATTTTGATTTAGATGTCCAAAATCATATTTTCTCAGGCGTCAATATCGATTCATCTGATCTTCTACCCCCCGCCATCGATTCATCTGATCTTCGACTCCCCATCATCGATTCATCTGATCTTCTACTCCCCACCATCGATTCATCTGATCTTCTACCCCCCACCACTATCCCGGGTTACACCACTTCATTAGGTGAGACCGGTGCTTCGACAATGCAATTAGGGGAATTTGGGTTCGAGGGTTTTGATGATCCGTGGGAATCATTCTTGAATAGCATTTGGCATAATGAAATACTTTCACCTGAAGATATTCAAAAGATGGGATAA
Protein sequence

>Msa0727520

MEYQSGGSGSFDSQSKKKRYRRPHTPNEIQRSKKKRYRRPHTPNQLEKLEEVFKECPKPDEKQKLQLSKELALSYGQIRFWFQNKRVQTKAKLERNDNRSLRAENDKIRCENISMKEALENSICPSGGCPPINGDCYFDRKRLRLENALLKEELYKESSIISEYTGRSVLGFPPVPPIQISSLDSTMGSIGQGLVGNSFDIGEIHNNHSSLHCPKGKEKLFNLSSLPSSGLLLMNEQLPQQSWTPRYQSMQVDTFGNAMSHAQYSGQDSAIVPPHFDLDVQNHIFSGVNIDSSDLLPPAIDSSDLRLPIIDSSDLLLPTIDSSDLLPPTTIPGYTTSLGETGASTMQLGEFGFEGFDDPWESFLNSIWHNEILSPEDIQKMG*