AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0729880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0729880 MtrunA17_Chr4g0009901 73.004 263 70 1 1 263 1 262 3.86e-138 392
Msa0729880 MtrunA17_Chr4g0009851 61.688 308 103 5 1 300 1 301 3.97e-129 370
Msa0729880 MtrunA17_Chr4g0009981 59.608 255 89 4 53 300 1 248 1.03e-96 286
Msa0729880 MtrunA17_Chr1g0161431 46.185 249 100 4 10 226 11 257 4.27e-66 218
Msa0729880 MtrunA17_Chr4g0011071 64.493 138 48 1 55 192 35 171 6.38e-60 189
Msa0729880 MtrunA17_Chr2g0294911 42.471 259 121 4 10 244 29 283 1.90e-59 200
Msa0729880 MtrunA17_Chr4g0067981 44.262 244 107 5 10 226 31 272 2.19e-57 196
Msa0729880 MtrunA17_Chr5g0423031 94.382 89 5 0 1 89 1 89 3.15e-57 182
Msa0729880 MtrunA17_Chr5g0423031 84.211 95 13 1 185 277 80 174 1.49e-41 142
Msa0729880 MtrunA17_Chr3g0123131 40.000 245 118 5 10 226 13 256 6.62e-53 182
Msa0729880 MtrunA17_Chr6g0471041 38.217 314 154 7 10 309 14 301 6.44e-52 176
Msa0729880 MtrunA17_Chr4g0009991 69.828 116 34 1 103 218 2 116 4.15e-50 162
Msa0729880 MtrunA17_Chr8g0376881 37.295 244 126 4 9 226 24 266 7.53e-49 171
Msa0729880 MtrunA17_Chr3g0137101 40.161 249 116 5 10 226 8 255 8.97e-49 172
Msa0729880 MtrunA17_Chr8g0391911 39.744 234 116 2 10 218 9 242 1.02e-48 163
Msa0729880 MtrunA17_Chr4g0010001 80.899 89 17 0 1 89 1 89 9.23e-48 155
Msa0729880 MtrunA17_Chr1g0149841 38.153 249 123 5 7 226 2 248 2.79e-47 166
Msa0729880 MtrunA17_Chr7g0275401 35.060 251 131 5 7 227 2 250 2.02e-44 153
Msa0729880 MtrunA17_Chr2g0304841 34.902 255 128 5 10 227 18 271 5.07e-44 159
Msa0729880 MtrunA17_Chr4g0074401 37.959 245 123 5 10 226 17 260 2.08e-43 157
Msa0729880 MtrunA17_Chr4g0074411 37.097 248 130 4 10 232 18 264 2.27e-43 157
Msa0729880 MtrunA17_Chr8g0383961 41.277 235 106 7 1 230 1 208 2.75e-42 147
Msa0729880 MtrunA17_Chr7g0275891 34.100 261 128 7 7 225 2 260 3.77e-42 149
Msa0729880 MtrunA17_Chr1g0149851 35.606 264 124 7 7 226 2 263 2.63e-40 147
Msa0729880 MtrunA17_Chr6g0456631 44.079 152 84 1 10 160 9 160 5.36e-39 135
Msa0729880 MtrunA17_Chr3g0134461 34.762 210 111 3 10 218 8 192 9.82e-38 132
Msa0729880 MtrunA17_Chr4g0074421 39.011 182 88 3 59 218 2 182 1.65e-35 127
Msa0729880 MtrunA17_Chr3g0134481 33.491 212 104 3 10 220 8 183 7.39e-34 122
Msa0729880 MtrunA17_Chr1g0149831 33.690 187 91 5 68 226 1 182 3.06e-25 101
Msa0729880 MtrunA17_Chr5g0417521 51.948 77 34 2 165 240 11 85 8.34e-17 80.1
Msa0729880 MtrunA17_Chr2g0321461 42.718 103 48 3 125 221 1 98 1.25e-16 79.7
Msa0729880 MtrunA17_Chr6g0473731 35.461 141 76 3 101 231 21 156 3.66e-16 78.6
Msa0729880 MtrunA17_Chr6g0461641 56.061 66 26 2 165 229 38 101 4.50e-16 78.2
Msa0729880 MtrunA17_Chr7g0261911 48.101 79 40 1 160 237 253 331 4.29e-15 75.5
Msa0729880 MtrunA17_Chr4g0031961 49.315 73 36 1 155 226 116 188 1.08e-14 73.6
Msa0729880 MtrunA17_Chr7g0255711 57.627 59 25 0 168 226 90 148 1.08e-14 73.2
Msa0729880 MtrunA17_Chr4g0042451 56.897 58 24 1 165 221 13 70 6.67e-14 71.2
Msa0729880 MtrunA17_Chr2g0283951 35.338 133 75 5 154 276 69 200 7.13e-14 71.6
Msa0729880 MtrunA17_Chr7g0274471 50.000 70 34 1 169 237 218 287 1.83e-13 70.5
Msa0729880 MtrunA17_Chr3g0095611 48.052 77 33 2 159 228 3 79 2.04e-13 68.2
Msa0729880 MtrunA17_Chr1g0190301 48.193 83 40 2 169 250 175 255 2.39e-13 70.1
Msa0729880 MtrunA17_Chr7g0243081 53.571 56 25 1 167 221 69 124 8.34e-13 68.2
Msa0729880 MtrunA17_Chr1g0162931 52.632 57 26 1 167 222 16 72 8.92e-13 67.4
Msa0729880 MtrunA17_Chr2g0324891 30.645 124 85 1 146 269 138 260 1.61e-12 67.8
Msa0729880 MtrunA17_Chr7g0254981 45.122 82 42 2 165 243 46 127 3.50e-12 65.9
Msa0729880 MtrunA17_Chr8g0356501 51.515 66 31 1 157 221 25 90 4.94e-12 65.9
Msa0729880 MtrunA17_Chr1g0197171 59.259 54 21 1 169 221 44 97 5.37e-12 65.5
Msa0729880 MtrunA17_Chr2g0310011 51.515 66 31 1 157 221 23 88 8.66e-12 65.1
Msa0729880 MtrunA17_Chr8g0379121 51.786 56 26 1 167 221 109 164 9.44e-12 65.5
Msa0729880 MtrunA17_Chr1g0210911 50.877 57 27 1 167 222 20 76 1.35e-11 64.3
Msa0729880 MtrunA17_Chr7g0258551 35.433 127 67 5 167 289 210 325 2.03e-11 64.3
Msa0729880 MtrunA17_Chr4g0054981 46.575 73 38 1 164 236 305 376 2.22e-11 64.3
Msa0729880 MtrunA17_Chr2g0290991 57.407 54 22 1 169 221 39 92 3.39e-11 63.2
Msa0729880 MtrunA17_Chr4g0040481 48.276 58 30 0 168 225 157 214 3.46e-11 63.5
Msa0729880 MtrunA17_Chr5g0400241 36.170 94 57 1 158 248 300 393 3.55e-11 63.9
Msa0729880 MtrunA17_Chr5g0400241 29.921 127 83 4 10 134 14 136 5.60e-11 63.2
Msa0729880 MtrunA17_Chr1g0175071 56.140 57 24 1 169 224 177 233 6.00e-11 62.4
Msa0729880 MtrunA17_Chr2g0288601 51.852 54 25 1 166 218 68 121 6.12e-11 59.3
Msa0729880 MtrunA17_Chr1g0175091 43.210 81 41 3 160 239 251 327 6.95e-11 62.8
Msa0729880 MtrunA17_Chr2g0320231 49.123 57 28 1 167 222 95 151 7.24e-11 62.4
Msa0729880 MtrunA17_Chr5g0409731 53.704 54 24 1 169 221 25 78 9.34e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0729880 AT3G16857.1 40.304 263 110 4 10 226 33 294 4.02e-56 192
Msa0729880 AT3G16857.2 40.304 263 110 4 10 226 33 294 6.02e-56 192
Msa0729880 AT2G01760.1 41.176 255 114 5 7 226 4 257 3.47e-54 181
Msa0729880 AT1G67710.1 40.000 245 117 5 11 226 8 251 5.18e-50 174
Msa0729880 AT2G01760.2 37.102 283 114 6 7 226 4 285 2.36e-48 167
Msa0729880 AT2G25180.1 38.589 241 123 4 10 226 13 252 1.79e-45 162
Msa0729880 AT4G31920.1 36.290 248 143 5 10 244 13 258 9.05e-43 155
Msa0729880 AT5G58080.1 37.597 258 132 6 10 244 14 265 3.49e-42 154
Msa0729880 AT3G16857.3 38.839 224 90 4 49 226 2 224 4.00e-42 154
Msa0729880 AT3G62670.1 33.202 253 131 8 12 227 23 274 1.31e-32 125
Msa0729880 AT2G01760.3 38.342 193 84 4 68 226 1 192 1.99e-32 122
Msa0729880 AT1G67710.2 37.968 187 87 4 68 226 1 186 8.68e-30 118
Msa0729880 AT5G49240.1 30.802 237 136 5 9 218 37 272 2.02e-29 114
Msa0729880 AT1G49190.1 30.894 246 132 7 10 221 30 271 7.60e-29 114
Msa0729880 AT5G07210.2 26.568 271 142 6 14 230 16 283 1.21e-22 98.6
Msa0729880 AT1G67710.3 37.126 167 76 4 88 226 12 177 3.59e-21 93.6
Msa0729880 AT5G07210.1 25.806 279 142 7 14 230 16 291 3.09e-20 91.7
Msa0729880 AT1G68210.1 25.556 270 143 7 8 221 7 274 1.45e-18 86.7
Msa0729880 AT1G49190.2 34.921 126 80 2 10 134 30 154 3.79e-17 82.4
Msa0729880 AT2G27070.1 24.825 286 151 9 14 242 16 294 3.58e-16 79.3
Msa0729880 AT3G04030.1 59.375 64 25 1 159 221 35 98 3.60e-16 79.0
Msa0729880 AT3G04030.3 59.375 64 25 1 159 221 35 98 3.85e-16 78.6
Msa0729880 AT5G18240.2 59.375 64 25 1 159 221 35 98 9.79e-16 77.4
Msa0729880 AT5G18240.3 59.375 64 25 1 159 221 35 98 9.79e-16 77.4
Msa0729880 AT5G18240.1 59.375 64 25 1 159 221 35 98 9.88e-16 77.4
Msa0729880 AT5G18240.4 59.375 64 25 1 159 221 35 98 9.88e-16 77.4
Msa0729880 AT5G18240.5 59.375 64 25 1 159 221 35 98 9.97e-16 77.4
Msa0729880 AT5G59570.2 49.315 73 34 1 157 226 126 198 2.49e-15 75.5
Msa0729880 AT5G59570.1 49.315 73 34 1 157 226 126 198 2.49e-15 75.5
Msa0729880 AT3G04030.2 55.556 63 28 0 159 221 35 97 3.81e-15 75.9
Msa0729880 AT2G40970.1 50.746 67 33 0 160 226 96 162 4.45e-15 73.9
Msa0729880 AT5G05090.1 51.429 70 33 1 158 226 69 138 4.90e-15 74.3
Msa0729880 AT3G46640.3 49.315 73 34 1 157 226 129 201 7.87e-15 74.3
Msa0729880 AT3G46640.2 49.315 73 34 1 157 226 129 201 1.23e-14 73.9
Msa0729880 AT3G46640.1 49.315 73 34 1 157 226 129 201 1.23e-14 73.9
Msa0729880 AT3G10760.1 57.627 59 25 0 168 226 104 162 1.68e-14 73.6
Msa0729880 AT2G01060.1 51.765 85 37 3 158 238 4 88 6.69e-14 71.2
Msa0729880 AT4G13640.1 42.308 104 50 3 169 268 37 134 1.22e-13 70.5
Msa0729880 AT4G13640.2 42.308 104 50 3 169 268 37 134 1.28e-13 70.5
Msa0729880 AT1G69580.1 38.095 126 67 5 165 284 30 150 1.29e-13 70.9
Msa0729880 AT5G29000.4 37.615 109 60 2 129 236 198 299 3.00e-13 69.7
Msa0729880 AT1G69580.2 40.187 107 57 4 165 269 30 131 3.90e-13 69.3
Msa0729880 AT4G04605.1 44.156 77 41 2 167 241 12 88 3.91e-13 68.2
Msa0729880 AT5G29000.2 36.607 112 63 2 129 239 198 302 5.79e-13 69.3
Msa0729880 AT5G44190.1 53.448 58 27 0 168 225 146 203 5.83e-13 69.3
Msa0729880 AT5G29000.1 36.607 112 63 2 129 239 155 259 6.40e-13 68.9
Msa0729880 AT5G29000.3 36.607 112 63 2 129 239 155 259 6.40e-13 68.9
Msa0729880 AT5G44190.2 53.448 58 27 0 168 225 146 203 6.77e-13 68.9
Msa0729880 AT3G24120.2 40.952 105 50 4 169 268 41 138 6.96e-13 68.6
Msa0729880 AT3G24120.1 40.952 105 50 4 169 268 41 138 7.05e-13 68.2
Msa0729880 AT5G06800.1 45.570 79 36 3 169 240 192 270 2.70e-12 67.4
Msa0729880 AT2G40260.1 56.140 57 24 1 167 222 80 136 3.56e-12 67.0
Msa0729880 AT3G04450.1 51.724 58 27 1 165 221 234 291 3.86e-12 67.0
Msa0729880 AT3G04450.4 50.847 59 28 1 165 222 194 252 4.22e-12 66.6
Msa0729880 AT3G04450.2 50.847 59 28 1 165 222 194 252 4.22e-12 66.6
Msa0729880 AT1G14600.1 58.491 53 21 1 170 221 24 76 4.55e-12 65.5
Msa0729880 AT3G04450.3 51.724 58 27 1 165 221 234 291 4.81e-12 66.2
Msa0729880 AT5G24470.1 29.134 127 85 3 13 135 49 174 5.12e-12 67.0
Msa0729880 AT5G06800.2 44.048 84 35 3 169 240 192 275 7.23e-12 65.9
Msa0729880 AT1G79430.2 43.299 97 51 3 157 252 22 115 1.59e-11 64.7
Msa0729880 AT2G38300.1 50.877 57 27 1 167 222 52 108 2.10e-11 64.3
Msa0729880 AT5G06800.3 52.381 63 28 2 161 221 183 245 2.88e-11 63.5
Msa0729880 AT4G28610.1 45.946 74 38 2 169 241 225 297 3.09e-11 64.3
Msa0729880 AT2G02060.2 48.387 62 31 1 170 230 30 91 3.21e-11 63.2
Msa0729880 AT2G02060.1 48.387 62 31 1 170 230 30 91 3.21e-11 63.2
Msa0729880 AT3G13040.3 37.607 117 63 5 168 281 240 349 4.02e-11 63.9
Msa0729880 AT3G13040.1 37.607 117 63 5 168 281 240 349 4.02e-11 63.9
Msa0729880 AT3G13040.2 37.607 117 63 5 168 281 240 349 4.02e-11 63.9
Msa0729880 AT5G45580.2 49.231 65 32 1 158 221 12 76 4.27e-11 62.8
Msa0729880 AT2G20570.1 43.750 80 39 1 152 225 130 209 4.44e-11 63.5
Msa0729880 AT2G42660.1 51.852 54 25 1 170 222 51 104 5.66e-11 62.4
Msa0729880 AT5G45580.1 55.556 54 23 1 169 221 7 60 5.95e-11 62.4
Msa0729880 AT2G20400.1 57.407 54 22 1 169 221 231 284 8.81e-11 62.8
Msa0729880 AT2G20400.2 57.407 54 22 1 169 221 231 284 8.91e-11 62.8

Find 62 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACAAAATTGGATGGAAAT+TGG 0.274934 5_1:-37855191 Msa0729880:CDS
CATATGGGGCTTGCGAATAT+TGG 0.282422 5_1:-37855900 Msa0729880:CDS
AATTCATCAGTTGATTATTT+CGG 0.292540 5_1:-37855101 Msa0729880:CDS
AAGATGATGCACAACAAAAT+TGG 0.295199 5_1:-37855202 Msa0729880:CDS
ACATGTTGGTAGTCATTTAC+AGG 0.298486 5_1:-37855496 Msa0729880:intron
GAATTAATCACGACACAATC+TGG 0.322128 5_1:+37855119 None:intergenic
TTCTGATGCCTTGAACTTGT+TGG 0.365455 5_1:-37856182 Msa0729880:CDS
GTTACAAAGTCTATCACATA+TGG 0.379871 5_1:-37855916 Msa0729880:CDS
GAACGTCATCATGTTGGTTC+CGG 0.381973 5_1:+37855267 None:intergenic
TCTTCAAAATATTGGAAGCT+TGG 0.394226 5_1:-37855800 Msa0729880:CDS
AATTCATAGGCATCCATGTT+TGG 0.395764 5_1:+37856115 None:intergenic
CTTGAACTTGTTGGAAAGAA+AGG 0.398808 5_1:-37856173 Msa0729880:CDS
GCAATAACGCTGAGACCATC+AGG 0.400667 5_1:+37856390 None:intergenic
ATCAGAAGCTGTATTGCATT+TGG 0.403201 5_1:+37856198 None:intergenic
CAAGTAACTAACCTTGATAA+TGG 0.405402 5_1:+37856313 None:intergenic
CTTTCTTTCCAACAAGTTCA+AGG 0.409488 5_1:+37856174 None:intergenic
CGGGAAAGAGAAGATGATAA+TGG 0.414808 5_1:-37855757 Msa0729880:CDS
CCCGCGGATAACTTTCATCT+TGG 0.418067 5_1:+37855228 None:intergenic
ACATCTCCTCAATGGTGTTA+AGG 0.419121 5_1:+37856346 None:intergenic
CTGAGACCATCAGGGAATGA+TGG 0.467245 5_1:+37856399 None:intergenic
ATTGGAAGCTTGGATGTTCA+AGG 0.468841 5_1:-37855790 Msa0729880:CDS
ATGTTCAAGGTCATAGAAAA+CGG 0.468876 5_1:-37855777 Msa0729880:CDS
TTACAAAGTCTATCACATAT+GGG 0.475108 5_1:-37855915 Msa0729880:CDS
TGGCATCATGTTGTAAGAAA+AGG 0.476984 5_1:-37855841 Msa0729880:CDS
TGATGGGTTGTCAATGAAAA+CGG 0.490079 5_1:+37856416 None:intergenic
TTGAAGCTCAGCGGATTGAC+CGG 0.492162 5_1:-37855286 Msa0729880:CDS
TTGGACCAAGCCGTTGTATG+AGG 0.493917 5_1:-37855881 Msa0729880:CDS
CTCAATGGTGTTAAGGAAAG+TGG 0.494366 5_1:+37856353 None:intergenic
GCCTCGTTTGTCATGGTCAC+CGG 0.494740 5_1:-37855710 Msa0729880:CDS
TGTTCAAGGTCATAGAAAAC+GGG 0.501689 5_1:-37855776 Msa0729880:CDS
AGCATGTTGAACAAATTCAT+AGG 0.506691 5_1:+37856102 None:intergenic
GATGTTCCTGGTTTGAGTGC+AGG 0.507577 5_1:-37855519 Msa0729880:CDS
ACACAATCTGGGAACAAGTT+GGG 0.508030 5_1:+37855131 None:intergenic
TTATGGGCTGTCAATCAACT+AGG 0.526348 5_1:-37855670 Msa0729880:CDS
TTGAATTGTTCCTCATACAA+CGG 0.530062 5_1:+37855871 None:intergenic
AGGAAAGAACCATACTATCA+AGG 0.531753 5_1:+37855647 None:intergenic
CACTTTCCTTAACACCATTG+AGG 0.536078 5_1:-37856352 Msa0729880:CDS
TGTAATCGATGCCATTATCA+AGG 0.541272 5_1:-37856324 Msa0729880:intron
GACACAATCTGGGAACAAGT+TGG 0.557153 5_1:+37855130 None:intergenic
TGATGCACAACAAAATTGGA+TGG 0.559172 5_1:-37855198 Msa0729880:CDS
TCGATTACACATCTCCTCAA+TGG 0.564409 5_1:+37856338 None:intergenic
AATTAATCACGACACAATCT+GGG 0.566059 5_1:+37855120 None:intergenic
TTTGTAACAGAACTTAATGT+GGG 0.567790 5_1:+37855931 None:intergenic
TGAGACCATCAGGGAATGAT+GGG 0.571071 5_1:+37856400 None:intergenic
CGAAGAAGCCTCGTTTGTCA+TGG 0.575576 5_1:-37855717 Msa0729880:CDS
AACAGGAATATTGAGTTGTA+CGG 0.581006 5_1:+37856078 None:intergenic
CTTTGTAACAGAACTTAATG+TGG 0.582713 5_1:+37855930 None:intergenic
GGTTTGAGTGCAGGACATGT+TGG 0.591175 5_1:-37855510 Msa0729880:CDS
ATGATGACGTTCAAGATGAG+TGG 0.593959 5_1:-37855256 Msa0729880:CDS
TTGTTCCTCATACAACGGCT+TGG 0.596667 5_1:+37855876 None:intergenic
ACATGTCCTGCACTCAAACC+AGG 0.599015 5_1:+37855513 None:intergenic
CATCTTGAACGTCATCATGT+TGG 0.599748 5_1:+37855261 None:intergenic
CCAAGATGAAAGTTATCCGC+GGG 0.608633 5_1:-37855228 Msa0729880:CDS
AGGAACAATTCAAGATGATG+TGG 0.609752 5_1:-37855861 Msa0729880:CDS
TGTGCATCATCTTGTACCCG+CGG 0.630231 5_1:+37855212 None:intergenic
GACAACCCATCATTCCCTGA+TGG 0.634739 5_1:-37856405 Msa0729880:CDS
TGATGTTCATATGCCAAACA+TGG 0.637365 5_1:-37856128 Msa0729880:CDS
CAATAACGCTGAGACCATCA+GGG 0.656279 5_1:+37856391 None:intergenic
TACAAAGTCTATCACATATG+GGG 0.656295 5_1:-37855914 Msa0729880:CDS
ACCAAGATGAAAGTTATCCG+CGG 0.659940 5_1:-37855229 Msa0729880:CDS
TCCGGTGACCATGACAAACG+AGG 0.694527 5_1:+37855709 None:intergenic
GTGATGACTTTGAAGCTCAG+CGG 0.696188 5_1:-37855295 Msa0729880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAATCTTCAAAATAT+TGG - chr5_1:37855709-37855728 Msa0729880:CDS 10.0%
!!! ATCTTTTTAATAATCATTTT+AGG + chr5_1:37855977-37855996 None:intergenic 10.0%
!! TAAAATGATTATTAAAAAGA+TGG - chr5_1:37855976-37855995 Msa0729880:intron 10.0%
!! AATAAAAATCTTCAAAATAT+TGG - chr5_1:37855709-37855728 Msa0729880:CDS 10.0%
!!! ATCTTTTTAATAATCATTTT+AGG + chr5_1:37855977-37855996 None:intergenic 10.0%
!! TAAAATGATTATTAAAAAGA+TGG - chr5_1:37855976-37855995 Msa0729880:intron 10.0%
!! TTTATGGAGTTTATTATAAT+TGG - chr5_1:37856115-37856134 Msa0729880:CDS 15.0%
!! TTTATGGAGTTTATTATAAT+TGG - chr5_1:37856115-37856134 Msa0729880:CDS 15.0%
!! AAGAAAAACTTACTAATAAC+AGG + chr5_1:37855459-37855478 None:intergenic 20.0%
!! AGTTTATTATAATTGGAATG+TGG - chr5_1:37856122-37856141 Msa0729880:CDS 20.0%
!! AATTCATCAGTTGATTATTT+CGG - chr5_1:37856416-37856435 Msa0729880:CDS 20.0%
!! AAGAAAAACTTACTAATAAC+AGG + chr5_1:37855459-37855478 None:intergenic 20.0%
!! AGTTTATTATAATTGGAATG+TGG - chr5_1:37856122-37856141 Msa0729880:CDS 20.0%
!! AATTCATCAGTTGATTATTT+CGG - chr5_1:37856416-37856435 Msa0729880:CDS 20.0%
! AACACTAGAATTGATGAATA+AGG + chr5_1:37855240-37855259 None:intergenic 25.0%
! TTTGTAACAGAACTTAATGT+GGG + chr5_1:37855589-37855608 None:intergenic 25.0%
! TTACAAAGTCTATCACATAT+GGG - chr5_1:37855602-37855621 Msa0729880:intron 25.0%
! ACAAAGCAAAGAAAAGAATT+AGG + chr5_1:37855893-37855912 None:intergenic 25.0%
! TTAAAATCACACAAAACATG+TGG + chr5_1:37856088-37856107 None:intergenic 25.0%
! AAAAAAGTTGCGAATATTGT+CGG + chr5_1:37856357-37856376 None:intergenic 25.0%
! AACACTAGAATTGATGAATA+AGG + chr5_1:37855240-37855259 None:intergenic 25.0%
! TTTGTAACAGAACTTAATGT+GGG + chr5_1:37855589-37855608 None:intergenic 25.0%
! TTACAAAGTCTATCACATAT+GGG - chr5_1:37855602-37855621 Msa0729880:intron 25.0%
! ACAAAGCAAAGAAAAGAATT+AGG + chr5_1:37855893-37855912 None:intergenic 25.0%
! TTAAAATCACACAAAACATG+TGG + chr5_1:37856088-37856107 None:intergenic 25.0%
! AAAAAAGTTGCGAATATTGT+CGG + chr5_1:37856357-37856376 None:intergenic 25.0%
CAAGTAACTAACCTTGATAA+TGG + chr5_1:37855207-37855226 None:intergenic 30.0%
! CATTATCAAGGTTAGTTACT+TGG - chr5_1:37855205-37855224 Msa0729880:CDS 30.0%
AGCATGTTGAACAAATTCAT+AGG + chr5_1:37855418-37855437 None:intergenic 30.0%
! AACAGGAATATTGAGTTGTA+CGG + chr5_1:37855442-37855461 None:intergenic 30.0%
CTTTGTAACAGAACTTAATG+TGG + chr5_1:37855590-37855609 None:intergenic 30.0%
GTTACAAAGTCTATCACATA+TGG - chr5_1:37855601-37855620 Msa0729880:intron 30.0%
TACAAAGTCTATCACATATG+GGG - chr5_1:37855603-37855622 Msa0729880:intron 30.0%
TTGAATTGTTCCTCATACAA+CGG + chr5_1:37855649-37855668 None:intergenic 30.0%
TCTTCAAAATATTGGAAGCT+TGG - chr5_1:37855717-37855736 Msa0729880:CDS 30.0%
ATGTTCAAGGTCATAGAAAA+CGG - chr5_1:37855740-37855759 Msa0729880:CDS 30.0%
! AACTGCATCAACGATTTTTA+TGG - chr5_1:37855830-37855849 Msa0729880:CDS 30.0%
! ACTGCATCAACGATTTTTAT+GGG - chr5_1:37855831-37855850 Msa0729880:CDS 30.0%
!! TTCTCTCACTTATTTTTCAG+AGG - chr5_1:37855946-37855965 Msa0729880:CDS 30.0%
ATTAAAAAGATGGATGTTCC+TGG - chr5_1:37855986-37856005 Msa0729880:intron 30.0%
TCGATTGATGAGAAAAAATG+TGG - chr5_1:37856179-37856198 Msa0729880:CDS 30.0%
CGATTGATGAGAAAAAATGT+GGG - chr5_1:37856180-37856199 Msa0729880:CDS 30.0%
AAGATGATGCACAACAAAAT+TGG - chr5_1:37856315-37856334 Msa0729880:intron 30.0%
CAACAAAATTGGATGGAAAT+TGG - chr5_1:37856326-37856345 Msa0729880:CDS 30.0%
AATTAATCACGACACAATCT+GGG + chr5_1:37856400-37856419 None:intergenic 30.0%
CAAGTAACTAACCTTGATAA+TGG + chr5_1:37855207-37855226 None:intergenic 30.0%
! CATTATCAAGGTTAGTTACT+TGG - chr5_1:37855205-37855224 Msa0729880:CDS 30.0%
AGCATGTTGAACAAATTCAT+AGG + chr5_1:37855418-37855437 None:intergenic 30.0%
! AACAGGAATATTGAGTTGTA+CGG + chr5_1:37855442-37855461 None:intergenic 30.0%
CTTTGTAACAGAACTTAATG+TGG + chr5_1:37855590-37855609 None:intergenic 30.0%
GTTACAAAGTCTATCACATA+TGG - chr5_1:37855601-37855620 Msa0729880:intron 30.0%
TACAAAGTCTATCACATATG+GGG - chr5_1:37855603-37855622 Msa0729880:intron 30.0%
TTGAATTGTTCCTCATACAA+CGG + chr5_1:37855649-37855668 None:intergenic 30.0%
TCTTCAAAATATTGGAAGCT+TGG - chr5_1:37855717-37855736 Msa0729880:CDS 30.0%
ATGTTCAAGGTCATAGAAAA+CGG - chr5_1:37855740-37855759 Msa0729880:CDS 30.0%
! AACTGCATCAACGATTTTTA+TGG - chr5_1:37855830-37855849 Msa0729880:CDS 30.0%
! ACTGCATCAACGATTTTTAT+GGG - chr5_1:37855831-37855850 Msa0729880:CDS 30.0%
!! TTCTCTCACTTATTTTTCAG+AGG - chr5_1:37855946-37855965 Msa0729880:CDS 30.0%
ATTAAAAAGATGGATGTTCC+TGG - chr5_1:37855986-37856005 Msa0729880:intron 30.0%
TCGATTGATGAGAAAAAATG+TGG - chr5_1:37856179-37856198 Msa0729880:CDS 30.0%
CGATTGATGAGAAAAAATGT+GGG - chr5_1:37856180-37856199 Msa0729880:CDS 30.0%
AAGATGATGCACAACAAAAT+TGG - chr5_1:37856315-37856334 Msa0729880:intron 30.0%
CAACAAAATTGGATGGAAAT+TGG - chr5_1:37856326-37856345 Msa0729880:CDS 30.0%
AATTAATCACGACACAATCT+GGG + chr5_1:37856400-37856419 None:intergenic 30.0%
!! TGATGGGTTGTCAATGAAAA+CGG + chr5_1:37855104-37855123 None:intergenic 35.0%
TGTAATCGATGCCATTATCA+AGG - chr5_1:37855193-37855212 Msa0729880:CDS 35.0%
ATCAGAAGCTGTATTGCATT+TGG + chr5_1:37855322-37855341 None:intergenic 35.0%
CTTTCTTTCCAACAAGTTCA+AGG + chr5_1:37855346-37855365 None:intergenic 35.0%
!! CTTGAACTTGTTGGAAAGAA+AGG - chr5_1:37855344-37855363 Msa0729880:CDS 35.0%
TGATGTTCATATGCCAAACA+TGG - chr5_1:37855389-37855408 Msa0729880:intron 35.0%
AATTCATAGGCATCCATGTT+TGG + chr5_1:37855405-37855424 None:intergenic 35.0%
ATTCAGTAGACATCACTACA+AGG + chr5_1:37855564-37855583 None:intergenic 35.0%
AGGAACAATTCAAGATGATG+TGG - chr5_1:37855656-37855675 Msa0729880:intron 35.0%
TGGCATCATGTTGTAAGAAA+AGG - chr5_1:37855676-37855695 Msa0729880:CDS 35.0%
TGTTCAAGGTCATAGAAAAC+GGG - chr5_1:37855741-37855760 Msa0729880:CDS 35.0%
AAAAATCGTTGATGCAGTTC+CGG + chr5_1:37855829-37855848 None:intergenic 35.0%
AGGAAAGAACCATACTATCA+AGG + chr5_1:37855873-37855892 None:intergenic 35.0%
!! ACATGTTGGTAGTCATTTAC+AGG - chr5_1:37856021-37856040 Msa0729880:intron 35.0%
TGATGCACAACAAAATTGGA+TGG - chr5_1:37856319-37856338 Msa0729880:intron 35.0%
GAATTAATCACGACACAATC+TGG + chr5_1:37856401-37856420 None:intergenic 35.0%
!! TGATGGGTTGTCAATGAAAA+CGG + chr5_1:37855104-37855123 None:intergenic 35.0%
TGTAATCGATGCCATTATCA+AGG - chr5_1:37855193-37855212 Msa0729880:CDS 35.0%
ATCAGAAGCTGTATTGCATT+TGG + chr5_1:37855322-37855341 None:intergenic 35.0%
CTTTCTTTCCAACAAGTTCA+AGG + chr5_1:37855346-37855365 None:intergenic 35.0%
!! CTTGAACTTGTTGGAAAGAA+AGG - chr5_1:37855344-37855363 Msa0729880:CDS 35.0%
TGATGTTCATATGCCAAACA+TGG - chr5_1:37855389-37855408 Msa0729880:intron 35.0%
AATTCATAGGCATCCATGTT+TGG + chr5_1:37855405-37855424 None:intergenic 35.0%
ATTCAGTAGACATCACTACA+AGG + chr5_1:37855564-37855583 None:intergenic 35.0%
AGGAACAATTCAAGATGATG+TGG - chr5_1:37855656-37855675 Msa0729880:intron 35.0%
TGGCATCATGTTGTAAGAAA+AGG - chr5_1:37855676-37855695 Msa0729880:CDS 35.0%
TGTTCAAGGTCATAGAAAAC+GGG - chr5_1:37855741-37855760 Msa0729880:CDS 35.0%
AAAAATCGTTGATGCAGTTC+CGG + chr5_1:37855829-37855848 None:intergenic 35.0%
AGGAAAGAACCATACTATCA+AGG + chr5_1:37855873-37855892 None:intergenic 35.0%
!! ACATGTTGGTAGTCATTTAC+AGG - chr5_1:37856021-37856040 Msa0729880:intron 35.0%
TGATGCACAACAAAATTGGA+TGG - chr5_1:37856319-37856338 Msa0729880:intron 35.0%
GAATTAATCACGACACAATC+TGG + chr5_1:37856401-37856420 None:intergenic 35.0%
! CTCAATGGTGTTAAGGAAAG+TGG + chr5_1:37855167-37855186 None:intergenic 40.0%
CACTTTCCTTAACACCATTG+AGG - chr5_1:37855165-37855184 Msa0729880:CDS 40.0%
! ACATCTCCTCAATGGTGTTA+AGG + chr5_1:37855174-37855193 None:intergenic 40.0%
TCGATTACACATCTCCTCAA+TGG + chr5_1:37855182-37855201 None:intergenic 40.0%
TTCTGATGCCTTGAACTTGT+TGG - chr5_1:37855335-37855354 Msa0729880:CDS 40.0%
ATTGGAAGCTTGGATGTTCA+AGG - chr5_1:37855727-37855746 Msa0729880:CDS 40.0%
CGGGAAAGAGAAGATGATAA+TGG - chr5_1:37855760-37855779 Msa0729880:CDS 40.0%
! TTATGGGCTGTCAATCAACT+AGG - chr5_1:37855847-37855866 Msa0729880:CDS 40.0%
! TCAACTAGGCCTTGATAGTA+TGG - chr5_1:37855861-37855880 Msa0729880:CDS 40.0%
CATCTTGAACGTCATCATGT+TGG + chr5_1:37856259-37856278 None:intergenic 40.0%
! ATGATGACGTTCAAGATGAG+TGG - chr5_1:37856261-37856280 Msa0729880:intron 40.0%
ACCAAGATGAAAGTTATCCG+CGG - chr5_1:37856288-37856307 Msa0729880:intron 40.0%
ACACAATCTGGGAACAAGTT+GGG + chr5_1:37856389-37856408 None:intergenic 40.0%
! CTCAATGGTGTTAAGGAAAG+TGG + chr5_1:37855167-37855186 None:intergenic 40.0%
CACTTTCCTTAACACCATTG+AGG - chr5_1:37855165-37855184 Msa0729880:CDS 40.0%
! ACATCTCCTCAATGGTGTTA+AGG + chr5_1:37855174-37855193 None:intergenic 40.0%
TCGATTACACATCTCCTCAA+TGG + chr5_1:37855182-37855201 None:intergenic 40.0%
TTCTGATGCCTTGAACTTGT+TGG - chr5_1:37855335-37855354 Msa0729880:CDS 40.0%
ATTGGAAGCTTGGATGTTCA+AGG - chr5_1:37855727-37855746 Msa0729880:CDS 40.0%
CGGGAAAGAGAAGATGATAA+TGG - chr5_1:37855760-37855779 Msa0729880:CDS 40.0%
! TTATGGGCTGTCAATCAACT+AGG - chr5_1:37855847-37855866 Msa0729880:CDS 40.0%
! TCAACTAGGCCTTGATAGTA+TGG - chr5_1:37855861-37855880 Msa0729880:CDS 40.0%
CATCTTGAACGTCATCATGT+TGG + chr5_1:37856259-37856278 None:intergenic 40.0%
! ATGATGACGTTCAAGATGAG+TGG - chr5_1:37856261-37856280 Msa0729880:intron 40.0%
ACCAAGATGAAAGTTATCCG+CGG - chr5_1:37856288-37856307 Msa0729880:intron 40.0%
ACACAATCTGGGAACAAGTT+GGG + chr5_1:37856389-37856408 None:intergenic 40.0%
TGAGACCATCAGGGAATGAT+GGG + chr5_1:37855120-37855139 None:intergenic 45.0%
CAATAACGCTGAGACCATCA+GGG + chr5_1:37855129-37855148 None:intergenic 45.0%
CATATGGGGCTTGCGAATAT+TGG - chr5_1:37855617-37855636 Msa0729880:intron 45.0%
TTGTTCCTCATACAACGGCT+TGG + chr5_1:37855644-37855663 None:intergenic 45.0%
!! GTGATGACTTTGAAGCTCAG+CGG - chr5_1:37856222-37856241 Msa0729880:intron 45.0%
!! GAACGTCATCATGTTGGTTC+CGG + chr5_1:37856253-37856272 None:intergenic 45.0%
CCAAGATGAAAGTTATCCGC+GGG - chr5_1:37856289-37856308 Msa0729880:intron 45.0%
GACACAATCTGGGAACAAGT+TGG + chr5_1:37856390-37856409 None:intergenic 45.0%
TGAGACCATCAGGGAATGAT+GGG + chr5_1:37855120-37855139 None:intergenic 45.0%
CAATAACGCTGAGACCATCA+GGG + chr5_1:37855129-37855148 None:intergenic 45.0%
CATATGGGGCTTGCGAATAT+TGG - chr5_1:37855617-37855636 Msa0729880:intron 45.0%
TTGTTCCTCATACAACGGCT+TGG + chr5_1:37855644-37855663 None:intergenic 45.0%
!! GTGATGACTTTGAAGCTCAG+CGG - chr5_1:37856222-37856241 Msa0729880:intron 45.0%
!! GAACGTCATCATGTTGGTTC+CGG + chr5_1:37856253-37856272 None:intergenic 45.0%
CCAAGATGAAAGTTATCCGC+GGG - chr5_1:37856289-37856308 Msa0729880:intron 45.0%
GACACAATCTGGGAACAAGT+TGG + chr5_1:37856390-37856409 None:intergenic 45.0%
!!! TGATTTTAAATTTAAATTTA+TGG - chr5_1:37856099-37856118 Msa0729880:CDS 5.0%
!!! TGATTTTAAATTTAAATTTA+TGG - chr5_1:37856099-37856118 Msa0729880:CDS 5.0%
GACAACCCATCATTCCCTGA+TGG - chr5_1:37855112-37855131 Msa0729880:CDS 50.0%
CTGAGACCATCAGGGAATGA+TGG + chr5_1:37855121-37855140 None:intergenic 50.0%
GCAATAACGCTGAGACCATC+AGG + chr5_1:37855130-37855149 None:intergenic 50.0%
TTGGACCAAGCCGTTGTATG+AGG - chr5_1:37855636-37855655 Msa0729880:intron 50.0%
CGAAGAAGCCTCGTTTGTCA+TGG - chr5_1:37855800-37855819 Msa0729880:CDS 50.0%
! GATGTTCCTGGTTTGAGTGC+AGG - chr5_1:37855998-37856017 Msa0729880:intron 50.0%
ACATGTCCTGCACTCAAACC+AGG + chr5_1:37856007-37856026 None:intergenic 50.0%
GGTTTGAGTGCAGGACATGT+TGG - chr5_1:37856007-37856026 Msa0729880:intron 50.0%
!! TTGAAGCTCAGCGGATTGAC+CGG - chr5_1:37856231-37856250 Msa0729880:intron 50.0%
CCCGCGGATAACTTTCATCT+TGG + chr5_1:37856292-37856311 None:intergenic 50.0%
TGTGCATCATCTTGTACCCG+CGG + chr5_1:37856308-37856327 None:intergenic 50.0%
GACAACCCATCATTCCCTGA+TGG - chr5_1:37855112-37855131 Msa0729880:CDS 50.0%
CTGAGACCATCAGGGAATGA+TGG + chr5_1:37855121-37855140 None:intergenic 50.0%
GCAATAACGCTGAGACCATC+AGG + chr5_1:37855130-37855149 None:intergenic 50.0%
TTGGACCAAGCCGTTGTATG+AGG - chr5_1:37855636-37855655 Msa0729880:intron 50.0%
CGAAGAAGCCTCGTTTGTCA+TGG - chr5_1:37855800-37855819 Msa0729880:CDS 50.0%
! GATGTTCCTGGTTTGAGTGC+AGG - chr5_1:37855998-37856017 Msa0729880:intron 50.0%
ACATGTCCTGCACTCAAACC+AGG + chr5_1:37856007-37856026 None:intergenic 50.0%
GGTTTGAGTGCAGGACATGT+TGG - chr5_1:37856007-37856026 Msa0729880:intron 50.0%
!! TTGAAGCTCAGCGGATTGAC+CGG - chr5_1:37856231-37856250 Msa0729880:intron 50.0%
CCCGCGGATAACTTTCATCT+TGG + chr5_1:37856292-37856311 None:intergenic 50.0%
TGTGCATCATCTTGTACCCG+CGG + chr5_1:37856308-37856327 None:intergenic 50.0%
GCCTCGTTTGTCATGGTCAC+CGG - chr5_1:37855807-37855826 Msa0729880:CDS 55.0%
TCCGGTGACCATGACAAACG+AGG + chr5_1:37855811-37855830 None:intergenic 55.0%
GCCTCGTTTGTCATGGTCAC+CGG - chr5_1:37855807-37855826 Msa0729880:CDS 55.0%
TCCGGTGACCATGACAAACG+AGG + chr5_1:37855811-37855830 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 37855097 37856442 37855097 ID=Msa0729880;Name=Msa0729880
chr5_1 mRNA 37855097 37856442 37855097 ID=Msa0729880-mRNA-1;Parent=Msa0729880;Name=Msa0729880-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0|1|1|1|5|0|313
chr5_1 exon 37856325 37856442 37856325 ID=Msa0729880-mRNA-1:exon:11394;Parent=Msa0729880-mRNA-1
chr5_1 exon 37856074 37856223 37856074 ID=Msa0729880-mRNA-1:exon:11393;Parent=Msa0729880-mRNA-1
chr5_1 exon 37855662 37855970 37855662 ID=Msa0729880-mRNA-1:exon:11392;Parent=Msa0729880-mRNA-1
chr5_1 exon 37855497 37855573 37855497 ID=Msa0729880-mRNA-1:exon:11391;Parent=Msa0729880-mRNA-1
chr5_1 exon 37855097 37855384 37855097 ID=Msa0729880-mRNA-1:exon:11390;Parent=Msa0729880-mRNA-1
chr5_1 CDS 37856325 37856442 37856325 ID=Msa0729880-mRNA-1:cds;Parent=Msa0729880-mRNA-1
chr5_1 CDS 37856074 37856223 37856074 ID=Msa0729880-mRNA-1:cds;Parent=Msa0729880-mRNA-1
chr5_1 CDS 37855662 37855970 37855662 ID=Msa0729880-mRNA-1:cds;Parent=Msa0729880-mRNA-1
chr5_1 CDS 37855497 37855573 37855497 ID=Msa0729880-mRNA-1:cds;Parent=Msa0729880-mRNA-1
chr5_1 CDS 37855097 37855384 37855097 ID=Msa0729880-mRNA-1:cds;Parent=Msa0729880-mRNA-1
Gene Sequence

>Msa0729880

ATGGCCGTTTTCATTGACAACCCATCATTCCCTGATGGTCTCAGCGTTATTGCAATTGATCATGACACCACTTTCCTTAACACCATTGAGGAGATGTGTAATCGATGCCATTATCAAGTTACCAAATGCAATACAGCTTCTGATGCCTTGAACTTGTTGGAAAGAAAGGACTGTTTTGATGTAATGTTAATTGATGTTCATATGCCAAACATGGATGCCTATGAATTTGTTCAACATGCTACCGTACAACTCAATATTCCTGTTATTATGATGTCTACTGAATATCCCACATTAAGTTCTGTTACAAAGTCTATCACATATGGGGCTTGCGAATATTGGACCAAGCCGTTGTATGAGGAACAATTCAAGATGATGTGGCATCATGTTGTAAGAAAAGGCTTGAGCAAAAATAAAAATCTTCAAAATATTGGAAGCTTGGATGTTCAAGGTCATAGAAAACGGGAAAGAGAAGATGATAATGGATCAAAAGAATCAAATGCGAAGAAGCCTCGTTTGTCATGGTCACCGGAACTGCATCAACGATTTTTATGGGCTGTCAATCAACTAGGCCTTGATAAGGCAAAACCTAAAATGATTATTAAAAAGATGGATGTTCCTGGTTTGAGTGCAGGACATGTTGGTAGTCATTTACAGAAATATAGAAACTATTTGAAGAGATCGATTGATGAGAAAAAATGTGGGAAGAAAAAATCAAAGCTGAGTGATGACTTTGAAGCTCAGCGGATTGACCGGAACCAACATGATGACGTTCAAGATGAGTGGATTGACCAAGATGAAAGTTATCCGCGGGTACAAGATGATGCACAACAAAATTGGATGGAAATTGGCAAATCCGACAATATTCGCAACTTTTTTTCTGACATACCCAACTTGTTCCCAGATTGTGTCGTGATTAATTCATCAGTTGATTATTTCGGCTAG
Protein sequence

>Msa0729880

MAVFIDNPSFPDGLSVIAIDHDTTFLNTIEEMCNRCHYQVTKCNTASDALNLLERKDCFDVMLIDVHMPNMDAYEFVQHATVQLNIPVIMMSTEYPTLSSVTKSITYGACEYWTKPLYEEQFKMMWHHVVRKGLSKNKNLQNIGSLDVQGHRKREREDDNGSKESNAKKPRLSWSPELHQRFLWAVNQLGLDKAKPKMIIKKMDVPGLSAGHVGSHLQKYRNYLKRSIDEKKCGKKKSKLSDDFEAQRIDRNQHDDVQDEWIDQDESYPRVQDDAQQNWMEIGKSDNIRNFFSDIPNLFPDCVVINSSVDYFG*