Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0730520 | XP_003614709.2 | 77.473 | 657 | 85 | 7 | 3 | 597 | 63 | 718 | 0.0 | 946 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0730520 | sp|O64556|Y2923_ARATH | 37.082 | 658 | 339 | 18 | 4 | 597 | 31 | 677 | 1.32e-120 | 380 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0730520 | MtrunA17_Chr5g0423881 | 73.487 | 347 | 66 | 5 | 3 | 324 | 63 | 408 | 1.35e-171 | 493 |
| Msa0730520 | MtrunA17_Chr8g0341061 | 43.510 | 678 | 288 | 19 | 4 | 598 | 61 | 726 | 4.94e-147 | 442 |
| Msa0730520 | MtrunA17_Chr8g0341101 | 43.491 | 676 | 291 | 19 | 4 | 597 | 60 | 726 | 5.67e-147 | 447 |
| Msa0730520 | MtrunA17_Chr5g0423891 | 85.985 | 264 | 16 | 1 | 355 | 597 | 9 | 272 | 9.08e-143 | 422 |
| Msa0730520 | MtrunA17_Chr8g0341011 | 41.931 | 663 | 298 | 22 | 4 | 597 | 35 | 679 | 1.65e-139 | 427 |
| Msa0730520 | MtrunA17_Chr8g0341151 | 41.033 | 658 | 305 | 17 | 4 | 594 | 25 | 666 | 2.01e-135 | 416 |
| Msa0730520 | MtrunA17_Chr8g0340931 | 40.569 | 668 | 300 | 20 | 4 | 597 | 31 | 675 | 3.43e-133 | 410 |
| Msa0730520 | MtrunA17_Chr8g0340881 | 41.203 | 665 | 301 | 23 | 4 | 597 | 35 | 680 | 3.19e-131 | 405 |
| Msa0730520 | MtrunA17_Chr8g0340871 | 39.367 | 663 | 319 | 17 | 4 | 597 | 37 | 685 | 3.53e-129 | 400 |
| Msa0730520 | MtrunA17_Chr8g0340911 | 38.695 | 659 | 321 | 18 | 4 | 597 | 35 | 675 | 2.72e-124 | 387 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0730520 | AT4G29990.2 | 39.189 | 666 | 322 | 16 | 4 | 597 | 28 | 682 | 6.59e-126 | 388 |
| Msa0730520 | AT4G29990.1 | 39.339 | 666 | 321 | 17 | 4 | 597 | 28 | 682 | 1.50e-124 | 388 |
| Msa0730520 | AT2G19230.2 | 37.082 | 658 | 339 | 18 | 4 | 597 | 31 | 677 | 1.34e-121 | 380 |
| Msa0730520 | AT2G19230.1 | 36.898 | 664 | 333 | 19 | 4 | 597 | 31 | 678 | 5.91e-120 | 380 |
| Msa0730520 | AT2G19210.1 | 37.651 | 664 | 333 | 19 | 4 | 597 | 32 | 684 | 6.80e-117 | 368 |
| Msa0730520 | AT2G19190.1 | 36.254 | 662 | 344 | 18 | 4 | 597 | 31 | 682 | 4.68e-108 | 345 |
| Msa0730520 | AT1G05700.1 | 37.255 | 663 | 331 | 17 | 4 | 597 | 32 | 678 | 4.99e-107 | 342 |
| Msa0730520 | AT2G14510.1 | 37.556 | 671 | 308 | 22 | 4 | 594 | 30 | 669 | 3.26e-106 | 340 |
| Msa0730520 | AT2G14510.2 | 37.556 | 671 | 308 | 22 | 4 | 594 | 38 | 677 | 4.66e-106 | 340 |
| Msa0730520 | AT5G59670.2 | 36.132 | 667 | 326 | 22 | 4 | 594 | 29 | 671 | 5.12e-106 | 339 |
Find 125 sgRNAs with CRISPR-Local
Find 589 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCCAGACGTATTGGAA+TGG | 0.579645 | 5_1:-39922865 | Msa0730520:CDS |
| AAACATGAATTCCAAAGAAC+TGG | 0.418218 | 5_1:+39928577 | None:intergenic |
| AAAGTTATGCATATTGAAAA+AGG | 0.232787 | 5_1:-39928398 | Msa0730520:CDS |
| AACCTTCAGCAACGGTTATC+AGG | 0.336402 | 5_1:-39923031 | Msa0730520:intron |
| AACCTTGGTCTTTGTCAGAC+AGG | 0.467312 | 5_1:-39925615 | Msa0730520:CDS |
| AAGCCCTTATGCTATATCTG+TGG | 0.661344 | 5_1:+39930375 | None:intergenic |
| AATCTCAAAGGCATTGATCA+TGG | 0.506074 | 5_1:+39928322 | None:intergenic |
| AATGAAAGGCACCCCTTGAT+TGG | 0.419774 | 5_1:+39930184 | None:intergenic |
| AATTGCTTCTTAATCTGACT+TGG | 0.445004 | 5_1:+39930467 | None:intergenic |
| ACACGGACGATGTATTTGGT+AGG | 0.664351 | 5_1:-39928733 | Msa0730520:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATTGCTTCTTAATCTGACT+TGG | + | chr5_1:39922305-39922324 | None:intergenic | 30.0% | |
| TGTCTTGTAAATACCAATCA+AGG | - | chr5_1:39922572-39922591 | Msa0730520:intron | 30.0% | |
| GTCTTGTAAATACCAATCAA+GGG | - | chr5_1:39922573-39922592 | Msa0730520:intron | 30.0% | |
| TCTTGTAAATACCAATCAAG+GGG | - | chr5_1:39922574-39922593 | Msa0730520:intron | 30.0% | |
| TCATTTCATTATTGGAACTC+TGG | - | chr5_1:39922603-39922622 | Msa0730520:intron | 30.0% | |
| ACTTGAAGAACTGTATTAAG+GGG | + | chr5_1:39923007-39923026 | None:intergenic | 30.0% | |
| TAAATACTAACTCATGGTAC+TGG | - | chr5_1:39923062-39923081 | Msa0730520:CDS | 30.0% | |
| AAATACTAACTCATGGTACT+GGG | - | chr5_1:39923063-39923082 | Msa0730520:CDS | 30.0% | |
| TCAACAACTTTCTTCTTCAA+TGG | + | chr5_1:39923181-39923200 | None:intergenic | 30.0% | |
| AAAAGTCTCTTTCCATATGA+GGG | + | chr5_1:39923620-39923639 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 39922103 | 39930688 | 39922103 | ID=Msa0730520;Name=Msa0730520 |
| chr5_1 | mRNA | 39922103 | 39930688 | 39922103 | ID=Msa0730520-mRNA-1;Parent=Msa0730520;Name=Msa0730520-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0|0|0.81|0.66|0.56|16|0|600 |
| chr5_1 | exon | 39930685 | 39930688 | 39930685 | ID=Msa0730520-mRNA-1:exon:11725;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39930453 | 39930626 | 39930453 | ID=Msa0730520-mRNA-1:exon:11724;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39930264 | 39930443 | 39930264 | ID=Msa0730520-mRNA-1:exon:11723;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39930068 | 39930233 | 39930068 | ID=Msa0730520-mRNA-1:exon:11722;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39928548 | 39928757 | 39928548 | ID=Msa0730520-mRNA-1:exon:11721;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39928276 | 39928514 | 39928276 | ID=Msa0730520-mRNA-1:exon:11720;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39926811 | 39926898 | 39926811 | ID=Msa0730520-mRNA-1:exon:11719;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39926280 | 39926351 | 39926280 | ID=Msa0730520-mRNA-1:exon:11718;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39926122 | 39926193 | 39926122 | ID=Msa0730520-mRNA-1:exon:11717;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39925966 | 39926043 | 39925966 | ID=Msa0730520-mRNA-1:exon:11716;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39925521 | 39925647 | 39925521 | ID=Msa0730520-mRNA-1:exon:11715;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39924650 | 39924835 | 39924650 | ID=Msa0730520-mRNA-1:exon:11714;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39923032 | 39923158 | 39923032 | ID=Msa0730520-mRNA-1:exon:11713;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39922828 | 39922890 | 39922828 | ID=Msa0730520-mRNA-1:exon:11712;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39922398 | 39922404 | 39922398 | ID=Msa0730520-mRNA-1:exon:11711;Parent=Msa0730520-mRNA-1 |
| chr5_1 | exon | 39922103 | 39922112 | 39922103 | ID=Msa0730520-mRNA-1:exon:11710;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39930685 | 39930688 | 39930685 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39930453 | 39930626 | 39930453 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39930264 | 39930443 | 39930264 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39930068 | 39930233 | 39930068 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39928548 | 39928757 | 39928548 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39928276 | 39928514 | 39928276 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39926811 | 39926898 | 39926811 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39926280 | 39926351 | 39926280 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39926122 | 39926193 | 39926122 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39925966 | 39926043 | 39925966 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39925521 | 39925647 | 39925521 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39924650 | 39924835 | 39924650 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39923032 | 39923158 | 39923032 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39922828 | 39922890 | 39922828 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39922398 | 39922404 | 39922398 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| chr5_1 | CDS | 39922103 | 39922112 | 39922103 | ID=Msa0730520-mRNA-1:cds;Parent=Msa0730520-mRNA-1 |
| Gene Sequence |
>Msa0730520 ATGAGTGTGATAAGCATTGATTGTGGGGTAAGTGAAAGTTACAAGGATAACACAAACAATTTTCCATATCAAGCCGATGATAACACCGTCGTCCGGTTTGGTCAAACCGGGAATATATCATCAGATTATGAAAATTCCAATCCAAGTCAGATTAAGAAGCAATTGAAGTCATTGAGAAATGGGAAGAGGAATTGTTATAACTTAAAACCTAAACAGGGAAAGAACCACAGATATAGCATAAGGGCTTATTTTCAATATGGAAACTATGACAACAAAAATAAAATACCAATTTTTGATCAGCATCTTGGAGTCAATCTTTGGGGGCAAGTAGAACTGGATTCAGCTACACAAATTTTACACACAATCGAATATTGTCTTGTAAATACCAATCAAGGGGTGCCTTTCATTTCATTATTGGAACTCTGGCCTTTGGGGGATTCTGTTTATCAAACATCATCAACTCTATTGCCTCTAATGCTCTTCACACGAATCAACCTTGGCGGGTCTATATTGCACTTCATCAGGTACACGGACGATGTATTTGGTAGGTCTTGGTTCAACAAAGAAGAATATGATATGAATCCAAAGAATACGTCATCATCTATTGATTTTGATACACTTGGTGACACTTACAAGCTTCCGACTGAGGTTTTGAACTCTGCAGTCGAGGCCGTAAACCTGTCCAGTTCTTTGGAATTCATGTTTAATTACATCAAAGATGAAGAGTATTATGTTAACCAGAAAAGGATAATGAATATTTCTATCAATGGTCTTGATGGGGTCACACTCACAGAACCCCTCACTCTTGCATACCAGAAGCCAACCACCAAAGTTATGCATATTGAAAAAGGGAAGGGCATTGATAATATTTTGATTGAAGCAACTTCTGATTCTGAGTTACCTGCCATGATCAATGCCTTTGAGATTTTCTGCATAATACCTGAGAACTTTTCTTCCACACAACAAGACGATGATTGGCAAGGAGACCCATGTGGACCAACAGGTTTTAGATGGGAAGGTCTGACTTGCGGCGGTGCGAATATTCCAAGAATAATTTCACTAAACTTGAGCTCAAGCAAATTGTCAGGCCGGATAGATGATGCATTCTCTAAACTCACAAATTTAGAGATTCTAGATTTGTCAAACAATGAACTGACAGGGAACCTTCCGGAATTTTTAGCACAATTACCGCGATTGAAAATCCTTAATTTGTCACGGAATAATCTCACAGGTCTCATTCCCAAGTCTCTAAAGGAGAAGTCAAATAATTCTTTGAAATTGAGTTTGGATGGAAACCTTGGTCTTTGTCAGACAGGCTCATGCAAAAGCAATAAAAAAAAATGGAGTGTTATACTAATTGTATCAATAGCAGCAACAGTTGCAGTCCTCATAATAGTTTGCGTTGCTGTGCTAAACACGAAATGTCGTGCATTTTCTTATTCGGAGGTTGTCAGTATCACTGATGATTTCAGACAAATGATTGGAAAAGGTGGATTTGGGAAGGTTTATCTAGGTATTATACCTGATGGTGAAAATGTTGCCGTGAAGACACTCTCTTTATCAGAATTGCAAGGGCATAAGGAGTTTATATCAGAGGTAAATTTGTTGATGCCTTCTCATCATAGAATTGTGGTTTCTCTTGTTGGTTATTGTGCGGATGGTGGCATTAGAGCACTTATTTTTGAATATCTCCCAGGCGGGAACCTTCAGCAACGGTTATCAGATAAAAACCCAGACGTATTGGAATGGAGTGAGAGACTTCAAATTGCATTTGATGTAGCAAATGAGTTATTTTCATTATGA |
| Protein sequence |
>Msa0730520 MSVISIDCGVSESYKDNTNNFPYQADDNTVVRFGQTGNISSDYENSNPSQIKKQLKSLRNGKRNCYNLKPKQGKNHRYSIRAYFQYGNYDNKNKIPIFDQHLGVNLWGQVELDSATQILHTIEYCLVNTNQGVPFISLLELWPLGDSVYQTSSTLLPLMLFTRINLGGSILHFIRYTDDVFGRSWFNKEEYDMNPKNTSSSIDFDTLGDTYKLPTEVLNSAVEAVNLSSSLEFMFNYIKDEEYYVNQKRIMNISINGLDGVTLTEPLTLAYQKPTTKVMHIEKGKGIDNILIEATSDSELPAMINAFEIFCIIPENFSSTQQDDDWQGDPCGPTGFRWEGLTCGGANIPRIISLNLSSSKLSGRIDDAFSKLTNLEILDLSNNELTGNLPEFLAQLPRLKILNLSRNNLTGLIPKSLKEKSNNSLKLSLDGNLGLCQTGSCKSNKKKWSVILIVSIAATVAVLIIVCVAVLNTKCRAFSYSEVVSITDDFRQMIGKGGFGKVYLGIIPDGENVAVKTLSLSELQGHKEFISEVNLLMPSHHRIVVSLVGYCADGGIRALIFEYLPGGNLQQRLSDKNPDVLEWSERLQIAFDVANELFSL* |
