AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0731030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0731030 MtrunA17_Chr5g0424801 70.033 307 81 5 1 296 9 315 7.53e-143 407
Msa0731030 MtrunA17_Chr5g0425261 70.033 307 81 5 1 296 9 315 1.14e-142 407
Msa0731030 MtrunA17_Chr5g0424931 55.556 333 106 8 3 296 18 347 4.35e-114 340
Msa0731030 MtrunA17_Chr1g0178011 56.825 315 112 8 1 296 9 318 1.33e-109 326
Msa0731030 MtrunA17_Chr5g0424741 54.519 343 108 6 1 296 9 350 9.94e-109 326
Msa0731030 MtrunA17_Chr2g0289221 52.215 316 124 9 1 298 17 323 3.29e-98 293
Msa0731030 MtrunA17_Chr5g0417281 49.518 311 135 7 1 296 8 311 3.94e-85 265
Msa0731030 MtrunA17_Chr5g0417211 49.206 315 132 9 1 296 9 314 1.95e-81 252
Msa0731030 MtrunA17_Chr2g0277531 45.307 309 143 9 4 296 20 318 1.06e-77 239
Msa0731030 MtrunA17_Chr2g0277461 46.624 311 141 9 4 296 13 316 4.31e-76 240
Msa0731030 MtrunA17_Chr5g0436761 44.479 317 146 10 1 296 9 316 7.15e-75 237
Msa0731030 MtrunA17_Chr5g0425661 56.566 198 64 7 3 183 36 228 2.06e-62 198
Msa0731030 MtrunA17_Chr3g0115271 37.006 354 156 12 1 296 16 360 1.81e-51 179
Msa0731030 MtrunA17_Chr5g0424791 65.714 140 44 2 53 188 12 151 4.18e-51 166
Msa0731030 MtrunA17_Chr2g0326761 34.078 358 164 13 1 296 8 355 5.30e-47 168
Msa0731030 MtrunA17_Chr1g0181211 33.056 360 173 9 1 296 24 379 1.24e-45 164
Msa0731030 MtrunA17_Chr1g0199681 33.149 362 163 14 1 297 91 438 1.24e-42 155
Msa0731030 MtrunA17_Chr4g0028971 33.242 364 165 11 1 296 9 362 5.11e-41 151
Msa0731030 MtrunA17_Chr7g0264161 31.746 315 168 10 14 296 2 301 4.31e-39 139
Msa0731030 MtrunA17_Chr7g0264171 29.144 374 172 12 1 296 28 386 2.72e-37 140
Msa0731030 MtrunA17_Chr2g0276781 32.622 328 170 12 4 296 23 334 2.12e-34 132
Msa0731030 MtrunA17_Chr2g0285461 33.535 331 172 15 1 296 20 337 7.48e-34 131
Msa0731030 MtrunA17_Chr8g0376771 32.628 331 174 14 1 296 20 336 1.02e-32 128
Msa0731030 MtrunA17_Chr1g0156321 31.186 295 181 7 1 274 40 333 1.29e-32 127
Msa0731030 MtrunA17_Chr2g0301251 31.790 324 185 12 1 296 20 335 1.91e-31 124
Msa0731030 MtrunA17_Chr8g0385791 31.329 316 191 9 4 296 59 371 2.75e-31 124
Msa0731030 MtrunA17_Chr5g0433301 31.832 333 175 15 1 296 21 338 5.02e-31 123
Msa0731030 MtrunA17_Chr2g0282961 29.667 300 176 10 4 275 53 345 3.05e-30 120
Msa0731030 MtrunA17_Chr3g0109391 30.723 332 180 14 1 296 23 340 6.20e-30 120
Msa0731030 MtrunA17_Chr4g0046371 29.703 303 177 9 4 275 38 335 9.96e-30 119
Msa0731030 MtrunA17_Chr4g0070181 31.481 324 186 13 1 296 20 335 4.02e-29 117
Msa0731030 MtrunA17_Chr2g0326281 31.270 307 156 10 4 260 56 357 5.55e-29 117
Msa0731030 MtrunA17_Chr8g0346711 30.671 313 194 11 4 296 29 338 6.80e-28 114
Msa0731030 MtrunA17_Chr7g0239061 29.969 327 179 13 4 296 14 324 2.97e-27 112
Msa0731030 MtrunA17_Chr4g0009751 30.031 323 188 13 2 296 17 329 8.65e-27 110
Msa0731030 MtrunA17_Chr8g0389081 33.040 227 108 7 1 197 29 241 8.86e-27 110
Msa0731030 MtrunA17_Chr4g0029671 30.612 294 172 8 4 267 54 345 1.09e-24 104
Msa0731030 MtrunA17_Chr5g0425651 53.933 89 37 2 211 296 1 88 6.32e-22 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0731030 AT1G77850.2 34.884 344 161 11 1 292 16 348 2.97e-55 189
Msa0731030 AT1G77850.1 34.884 344 161 11 1 292 16 348 3.97e-55 189
Msa0731030 AT4G30080.1 32.869 359 167 12 1 296 16 363 3.33e-43 157
Msa0731030 AT2G28350.2 32.353 374 156 16 1 296 7 361 2.24e-38 144
Msa0731030 AT2G28350.1 32.353 374 156 16 1 296 7 361 2.77e-38 144
Msa0731030 AT5G37020.2 31.845 336 171 16 1 296 19 336 4.69e-34 132
Msa0731030 AT2G46530.3 32.298 322 179 13 3 296 41 351 4.75e-34 131
Msa0731030 AT2G46530.1 32.298 322 179 13 3 296 20 330 6.13e-34 131
Msa0731030 AT5G37020.1 31.845 336 171 16 1 296 19 336 8.19e-34 131
Msa0731030 AT3G61830.1 32.817 323 176 14 3 296 24 334 9.49e-34 130
Msa0731030 AT1G30330.3 32.424 330 177 14 1 296 22 339 1.79e-32 127
Msa0731030 AT1G30330.2 32.424 330 177 14 1 296 22 339 1.79e-32 127
Msa0731030 AT1G30330.1 32.424 330 177 14 1 296 20 337 2.02e-32 127
Msa0731030 AT2G33860.1 32.432 296 159 12 4 268 54 339 2.33e-31 124
Msa0731030 AT1G59750.4 32.177 317 186 11 4 296 22 333 3.64e-31 123
Msa0731030 AT1G59750.2 32.177 317 186 11 4 296 22 333 3.95e-31 123
Msa0731030 AT1G59750.1 32.177 317 186 11 4 296 22 333 4.30e-31 123
Msa0731030 AT1G59750.3 32.177 317 186 11 4 296 22 333 4.30e-31 123
Msa0731030 AT1G19850.1 35.217 230 125 7 1 213 51 273 1.55e-29 119
Msa0731030 AT1G19850.2 35.217 230 125 7 1 213 40 262 1.55e-29 119
Msa0731030 AT5G62000.4 30.031 323 186 10 4 296 61 373 1.88e-29 119
Msa0731030 AT5G62000.2 30.031 323 186 10 4 296 61 373 2.94e-29 118
Msa0731030 AT5G62000.5 30.031 323 186 10 4 296 61 373 2.94e-29 118
Msa0731030 AT5G62000.3 30.031 323 186 10 4 296 61 373 2.94e-29 118
Msa0731030 AT5G62000.1 30.031 323 186 10 4 296 61 373 2.94e-29 118
Msa0731030 AT1G34390.1 30.892 314 179 11 1 292 24 321 6.72e-29 117
Msa0731030 AT1G35520.1 31.013 316 178 12 1 292 24 323 1.33e-28 115
Msa0731030 AT1G35540.1 30.128 312 186 11 1 292 24 323 2.16e-28 115
Msa0731030 AT5G20730.3 31.385 325 186 13 1 296 21 337 2.44e-28 115
Msa0731030 AT1G34310.1 31.629 313 174 12 4 292 27 323 2.44e-28 115
Msa0731030 AT5G20730.1 31.385 325 186 13 1 296 21 337 3.05e-28 115
Msa0731030 AT1G19220.1 30.488 328 184 13 1 296 20 335 3.19e-28 115
Msa0731030 AT5G20730.2 31.481 324 186 13 1 296 21 336 3.64e-28 115
Msa0731030 AT1G35240.1 30.696 316 179 12 1 292 24 323 8.83e-28 113
Msa0731030 AT1G34410.1 29.524 315 184 11 1 292 24 323 1.10e-27 113
Msa0731030 AT5G60450.1 30.606 330 199 10 3 303 64 392 6.31e-27 111
Msa0731030 AT5G60450.2 30.606 330 199 10 3 303 40 368 6.70e-27 111
Msa0731030 AT4G23980.1 28.797 316 184 11 4 292 12 313 8.43e-27 110
Msa0731030 AT4G23980.2 28.797 316 184 11 4 292 12 313 9.06e-27 110
Msa0731030 AT1G34170.1 28.931 318 184 13 1 292 22 323 4.90e-23 99.8
Msa0731030 AT1G43950.1 33.333 207 114 7 1 191 24 222 1.65e-22 94.0
Msa0731030 AT3G61830.2 31.449 283 157 13 39 296 13 283 1.98e-20 92.0
Msa0731030 AT1G34170.2 30.980 255 142 10 1 229 22 268 4.77e-20 90.9
Msa0731030 AT1G34170.3 30.980 255 142 10 1 229 22 268 5.14e-20 90.9
Msa0731030 AT2G33860.2 31.183 186 102 8 104 268 2 182 1.07e-15 77.8
Msa0731030 AT5G37020.3 30.400 250 130 13 77 296 6 241 2.85e-15 77.0
Msa0731030 AT2G46530.2 31.163 215 120 10 103 296 36 243 1.17e-13 71.6

Find 77 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTCCATCGTCGTCTTCT+TGG 0.207983 5_1:+41100461 None:intergenic
ATAGTTAAATTTGAAGATTC+AGG 0.238302 5_1:+41099944 None:intergenic
TAAACTTTAGAGTGAATCTT+TGG 0.260426 5_1:+41100688 None:intergenic
ATTCTTCTAATGTCCCTAAT+TGG 0.268826 5_1:-41099886 Msa0731030:CDS
TCTTGAGACTTCTGCAACTC+TGG 0.293011 5_1:+41100352 None:intergenic
ATGGTGATTGTGGCAGCATT+AGG 0.295879 5_1:+41100625 None:intergenic
ACCTTAAGCATGCGCCAATT+AGG 0.312806 5_1:+41099872 None:intergenic
CTTGGTCTGTTACGGGAGTT+AGG 0.314595 5_1:+41100509 None:intergenic
CTACTTCCCACAAGGTCATT+TGG 0.318755 5_1:-41100665 Msa0731030:CDS
ATGAAGATTGGTTGGAAATC+TGG 0.337904 5_1:-41099995 Msa0731030:CDS
TCTTCAAATTTAACTATAAG+AGG 0.347983 5_1:-41099938 Msa0731030:CDS
TGGTGATTGTGGCAGCATTA+GGG 0.354813 5_1:+41100626 None:intergenic
AGCGCATTTCTGCCATAGTT+TGG 0.354882 5_1:+41100729 None:intergenic
GATGAAAACAACGGAATCTT+CGG 0.357948 5_1:+41100222 None:intergenic
TTCTCACCTCTTTCAGCTGC+AGG 0.358959 5_1:+41100148 None:intergenic
CCCTAATTGGCGCATGCTTA+AGG 0.360706 5_1:-41099873 Msa0731030:CDS
GAGGGCAAAGCTTCGTTTGA+TGG 0.363032 5_1:-41099969 Msa0731030:CDS
GTCTCTGTCACTGACCTTCC+AGG 0.363192 5_1:-41100328 Msa0731030:CDS
CCACAGGAGGAGGAGGTTCT+TGG 0.364923 5_1:+41100491 None:intergenic
GCCACAATCACCATTCTTGA+TGG 0.366631 5_1:-41100616 Msa0731030:CDS
CCTTAAGCATGCGCCAATTA+GGG 0.368474 5_1:+41099873 None:intergenic
ATTAACTGAGAGTGCGGTCT+TGG 0.368633 5_1:-41100125 Msa0731030:CDS
AGGAGGTTCTTGGTCTGTTA+CGG 0.370719 5_1:+41100501 None:intergenic
AGATTTCCAACCAATCTTCA+TGG 0.383662 5_1:+41099997 None:intergenic
ATGAAAACAACGGAATCTTC+GGG 0.384466 5_1:+41100223 None:intergenic
GGAGGTTCTTGGTCTGTTAC+GGG 0.397553 5_1:+41100502 None:intergenic
ACACTAACTGATACTAAATC+TGG 0.404848 5_1:-41100412 Msa0731030:CDS
GGTCTTGGGTGCAGTGGATT+TGG 0.411620 5_1:-41100110 Msa0731030:CDS
TTTATCCCACAGATGGGAAT+TGG 0.411809 5_1:-41100057 Msa0731030:CDS
CTCCTATGGACTCCAAACTA+TGG 0.424679 5_1:-41100741 None:intergenic
TACGGGAGTTAGGAGCAGTT+TGG 0.451525 5_1:+41100519 None:intergenic
ATCTTTGGAATCTTGACGTC+GGG 0.453371 5_1:+41100703 None:intergenic
TTAACTGAGAGTGCGGTCTT+GGG 0.478339 5_1:-41100124 Msa0731030:CDS
AACTTCCCTGCAGCTGAAAG+AGG 0.482726 5_1:-41100154 Msa0731030:CDS
GTATAGGTGTAGACTTTGCC+TGG 0.494583 5_1:+41100310 None:intergenic
GTAGATGATGCCATGAAGAT+TGG 0.497274 5_1:-41100007 Msa0731030:CDS
CATGGCATCATCTACAACCT+TGG 0.499201 5_1:+41100015 None:intergenic
TCTTCTTGGCCGTGAACCAC+AGG 0.514079 5_1:+41100475 None:intergenic
CCTCCTCCTCCTGTGGTTCA+CGG 0.519022 5_1:-41100484 Msa0731030:CDS
AGGTGTAGACTTTGCCTGGA+AGG 0.536557 5_1:+41100314 None:intergenic
TGGCCGTGAACCACAGGAGG+AGG 0.540091 5_1:+41100481 None:intergenic
CCATGCATCGTCTCTACTGT+TGG 0.543464 5_1:-41100577 Msa0731030:CDS
AAATATATGTCGGAATTCGC+AGG 0.543716 5_1:-41100183 Msa0731030:CDS
GAGACGATGCATGGAATGGA+TGG 0.546365 5_1:+41100586 None:intergenic
TTGGTTGGAAATCTGGAATG+AGG 0.547760 5_1:-41099988 Msa0731030:CDS
ATGATGCCATGAAGATTGGT+TGG 0.558711 5_1:-41100003 Msa0731030:CDS
CCGTGAACCACAGGAGGAGG+AGG 0.561880 5_1:+41100484 None:intergenic
GAGTGCGGTCTTGGGTGCAG+TGG 0.564030 5_1:-41100116 Msa0731030:CDS
AAACTATGGCAGAAATGCGC+TGG 0.567239 5_1:-41100727 Msa0731030:CDS
ATTACACCAATTCCCATCTG+TGG 0.570163 5_1:+41100051 None:intergenic
AGTAGAGACGATGCATGGAA+TGG 0.572932 5_1:+41100582 None:intergenic
AAACAGAGTAGACTAACTAA+AGG 0.575699 5_1:-41100280 Msa0731030:CDS
TCTCACCTCTTTCAGCTGCA+GGG 0.578496 5_1:+41100149 None:intergenic
CATGTTCCAAATGACCTTGT+GGG 0.588081 5_1:+41100659 None:intergenic
GGTGATTGTGGCAGCATTAG+GGG 0.588227 5_1:+41100627 None:intergenic
TGGTTGGAAATCTGGAATGA+GGG 0.589287 5_1:-41099987 Msa0731030:CDS
TGGACGGTAACCATCAAGAA+TGG 0.589526 5_1:+41100606 None:intergenic
ACCATCAAGAATGGTGATTG+TGG 0.590838 5_1:+41100615 None:intergenic
CCAAGAACCTCCTCCTCCTG+TGG 0.595254 5_1:-41100491 Msa0731030:CDS
TTACACCAATTCCCATCTGT+GGG 0.595749 5_1:+41100052 None:intergenic
TGCCATAGTTTGGAGTCCAT+AGG 0.605278 5_1:+41100739 None:intergenic
ATCAAGTCTGTGCATTGACG+AGG 0.606145 5_1:+41100379 None:intergenic
GCAAAGACCTCGTCGGTGTG+AGG 0.608714 5_1:+41100541 None:intergenic
CGATGCATGGAATGGATGGA+CGG 0.620709 5_1:+41100590 None:intergenic
AGAGTAGACTAACTAAAGGA+TGG 0.627717 5_1:-41100276 Msa0731030:CDS
GCATGTTCCAAATGACCTTG+TGG 0.633704 5_1:+41100658 None:intergenic
TAGTGTGAGAGTCTTGACAT+AGG 0.637006 5_1:+41100429 None:intergenic
AATCTTTGGAATCTTGACGT+CGG 0.641188 5_1:+41100702 None:intergenic
CCAACAGTAGAGACGATGCA+TGG 0.649094 5_1:+41100577 None:intergenic
TCTTGGCCGTGAACCACAGG+AGG 0.650102 5_1:+41100478 None:intergenic
TGTTGATCCTCACACCGACG+AGG 0.655379 5_1:-41100548 Msa0731030:CDS
CAGTTTGGCAAAGACCTCGT+CGG 0.663811 5_1:+41100534 None:intergenic
AAAGTTTACTACTTCCCACA+AGG 0.678948 5_1:-41100673 Msa0731030:CDS
TGAGAAATTAACTGAGAGTG+CGG 0.683957 5_1:-41100131 Msa0731030:CDS
TGAAAACAACGGAATCTTCG+GGG 0.698750 5_1:+41100224 None:intergenic
CACGGCCAAGAAGACGACGA+TGG 0.706758 5_1:-41100466 Msa0731030:CDS
GGATTTGGCTAACAAGAACG+TGG 0.730069 5_1:-41100095 Msa0731030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTTAAATTTGAAGATTC+AGG + chr5_1:41100600-41100619 None:intergenic 20.0%
!! TCTTCAAATTTAACTATAAG+AGG - chr5_1:41100603-41100622 Msa0731030:CDS 20.0%
!! ATAGTTAAATTTGAAGATTC+AGG + chr5_1:41100600-41100619 None:intergenic 20.0%
!! TCTTCAAATTTAACTATAAG+AGG - chr5_1:41100603-41100622 Msa0731030:CDS 20.0%
!!! TAAACTTTAGAGTGAATCTT+TGG + chr5_1:41099856-41099875 None:intergenic 25.0%
!! AGAGTTTTTGATGAAAACAA+CGG + chr5_1:41100331-41100350 None:intergenic 25.0%
!! AATTCCGACATATATTTTTC+CGG + chr5_1:41100355-41100374 None:intergenic 25.0%
!!! GCTTTCTTATTTTGTTCTAA+TGG - chr5_1:41100698-41100717 Msa0731030:CDS 25.0%
!!! TAAACTTTAGAGTGAATCTT+TGG + chr5_1:41099856-41099875 None:intergenic 25.0%
!! AGAGTTTTTGATGAAAACAA+CGG + chr5_1:41100331-41100350 None:intergenic 25.0%
!! AATTCCGACATATATTTTTC+CGG + chr5_1:41100355-41100374 None:intergenic 25.0%
!!! GCTTTCTTATTTTGTTCTAA+TGG - chr5_1:41100698-41100717 Msa0731030:CDS 25.0%
ACACTAACTGATACTAAATC+TGG - chr5_1:41100129-41100148 Msa0731030:CDS 30.0%
AAACAGAGTAGACTAACTAA+AGG - chr5_1:41100261-41100280 Msa0731030:CDS 30.0%
! GAATTGGTGTAATTTTGTAG+TGG - chr5_1:41100500-41100519 Msa0731030:CDS 30.0%
! TAACTATAAGAGGTTTTCAG+CGG - chr5_1:41100613-41100632 Msa0731030:CDS 30.0%
ATTCTTCTAATGTCCCTAAT+TGG - chr5_1:41100655-41100674 Msa0731030:CDS 30.0%
ACACTAACTGATACTAAATC+TGG - chr5_1:41100129-41100148 Msa0731030:CDS 30.0%
AAACAGAGTAGACTAACTAA+AGG - chr5_1:41100261-41100280 Msa0731030:CDS 30.0%
! GAATTGGTGTAATTTTGTAG+TGG - chr5_1:41100500-41100519 Msa0731030:CDS 30.0%
! TAACTATAAGAGGTTTTCAG+CGG - chr5_1:41100613-41100632 Msa0731030:CDS 30.0%
ATTCTTCTAATGTCCCTAAT+TGG - chr5_1:41100655-41100674 Msa0731030:CDS 30.0%
!! AATCTTTGGAATCTTGACGT+CGG + chr5_1:41099842-41099861 None:intergenic 35.0%
AAAGTTTACTACTTCCCACA+AGG - chr5_1:41099868-41099887 Msa0731030:CDS 35.0%
!! ACTCTGTTTTGAGTAGGTAT+AGG + chr5_1:41100250-41100269 None:intergenic 35.0%
!! TAGTCTACTCTGTTTTGAGT+AGG + chr5_1:41100256-41100275 None:intergenic 35.0%
AGAGTAGACTAACTAAAGGA+TGG - chr5_1:41100265-41100284 Msa0731030:CDS 35.0%
ATGAAAACAACGGAATCTTC+GGG + chr5_1:41100321-41100340 None:intergenic 35.0%
GATGAAAACAACGGAATCTT+CGG + chr5_1:41100322-41100341 None:intergenic 35.0%
! GTTTTCATCAAAAACTCTGC+CGG - chr5_1:41100333-41100352 Msa0731030:CDS 35.0%
TCTGCCGGAAAAATATATGT+CGG - chr5_1:41100348-41100367 Msa0731030:CDS 35.0%
AAATATATGTCGGAATTCGC+AGG - chr5_1:41100358-41100377 Msa0731030:CDS 35.0%
TGAGAAATTAACTGAGAGTG+CGG - chr5_1:41100410-41100429 Msa0731030:CDS 35.0%
! TTGTGTTTTATCCCACAGAT+GGG - chr5_1:41100478-41100497 Msa0731030:CDS 35.0%
!!! TAATTTTGTAGTGGATGCCA+AGG - chr5_1:41100509-41100528 Msa0731030:CDS 35.0%
AGATTTCCAACCAATCTTCA+TGG + chr5_1:41100547-41100566 None:intergenic 35.0%
! ATGAAGATTGGTTGGAAATC+TGG - chr5_1:41100546-41100565 Msa0731030:CDS 35.0%
! AACTATAAGAGGTTTTCAGC+GGG - chr5_1:41100614-41100633 Msa0731030:CDS 35.0%
!! AATCTTTGGAATCTTGACGT+CGG + chr5_1:41099842-41099861 None:intergenic 35.0%
AAAGTTTACTACTTCCCACA+AGG - chr5_1:41099868-41099887 Msa0731030:CDS 35.0%
!! ACTCTGTTTTGAGTAGGTAT+AGG + chr5_1:41100250-41100269 None:intergenic 35.0%
!! TAGTCTACTCTGTTTTGAGT+AGG + chr5_1:41100256-41100275 None:intergenic 35.0%
AGAGTAGACTAACTAAAGGA+TGG - chr5_1:41100265-41100284 Msa0731030:CDS 35.0%
ATGAAAACAACGGAATCTTC+GGG + chr5_1:41100321-41100340 None:intergenic 35.0%
GATGAAAACAACGGAATCTT+CGG + chr5_1:41100322-41100341 None:intergenic 35.0%
! GTTTTCATCAAAAACTCTGC+CGG - chr5_1:41100333-41100352 Msa0731030:CDS 35.0%
TCTGCCGGAAAAATATATGT+CGG - chr5_1:41100348-41100367 Msa0731030:CDS 35.0%
AAATATATGTCGGAATTCGC+AGG - chr5_1:41100358-41100377 Msa0731030:CDS 35.0%
TGAGAAATTAACTGAGAGTG+CGG - chr5_1:41100410-41100429 Msa0731030:CDS 35.0%
! TTGTGTTTTATCCCACAGAT+GGG - chr5_1:41100478-41100497 Msa0731030:CDS 35.0%
!!! TAATTTTGTAGTGGATGCCA+AGG - chr5_1:41100509-41100528 Msa0731030:CDS 35.0%
AGATTTCCAACCAATCTTCA+TGG + chr5_1:41100547-41100566 None:intergenic 35.0%
! ATGAAGATTGGTTGGAAATC+TGG - chr5_1:41100546-41100565 Msa0731030:CDS 35.0%
! AACTATAAGAGGTTTTCAGC+GGG - chr5_1:41100614-41100633 Msa0731030:CDS 35.0%
!! ATCTTTGGAATCTTGACGTC+GGG + chr5_1:41099841-41099860 None:intergenic 40.0%
CATGTTCCAAATGACCTTGT+GGG + chr5_1:41099885-41099904 None:intergenic 40.0%
ACCATCAAGAATGGTGATTG+TGG + chr5_1:41099929-41099948 None:intergenic 40.0%
TAGTGTGAGAGTCTTGACAT+AGG + chr5_1:41100115-41100134 None:intergenic 40.0%
TGAAAACAACGGAATCTTCG+GGG + chr5_1:41100320-41100339 None:intergenic 40.0%
! GTTGTGTTTTATCCCACAGA+TGG - chr5_1:41100477-41100496 Msa0731030:CDS 40.0%
TTTATCCCACAGATGGGAAT+TGG - chr5_1:41100484-41100503 Msa0731030:CDS 40.0%
TTACACCAATTCCCATCTGT+GGG + chr5_1:41100492-41100511 None:intergenic 40.0%
ATTACACCAATTCCCATCTG+TGG + chr5_1:41100493-41100512 None:intergenic 40.0%
GTAGATGATGCCATGAAGAT+TGG - chr5_1:41100534-41100553 Msa0731030:CDS 40.0%
ATGATGCCATGAAGATTGGT+TGG - chr5_1:41100538-41100557 Msa0731030:CDS 40.0%
! TTGGTTGGAAATCTGGAATG+AGG - chr5_1:41100553-41100572 Msa0731030:CDS 40.0%
! TGGTTGGAAATCTGGAATGA+GGG - chr5_1:41100554-41100573 Msa0731030:CDS 40.0%
!! ATCTTTGGAATCTTGACGTC+GGG + chr5_1:41099841-41099860 None:intergenic 40.0%
CATGTTCCAAATGACCTTGT+GGG + chr5_1:41099885-41099904 None:intergenic 40.0%
ACCATCAAGAATGGTGATTG+TGG + chr5_1:41099929-41099948 None:intergenic 40.0%
TAGTGTGAGAGTCTTGACAT+AGG + chr5_1:41100115-41100134 None:intergenic 40.0%
TGAAAACAACGGAATCTTCG+GGG + chr5_1:41100320-41100339 None:intergenic 40.0%
! GTTGTGTTTTATCCCACAGA+TGG - chr5_1:41100477-41100496 Msa0731030:CDS 40.0%
TTTATCCCACAGATGGGAAT+TGG - chr5_1:41100484-41100503 Msa0731030:CDS 40.0%
TTACACCAATTCCCATCTGT+GGG + chr5_1:41100492-41100511 None:intergenic 40.0%
ATTACACCAATTCCCATCTG+TGG + chr5_1:41100493-41100512 None:intergenic 40.0%
GTAGATGATGCCATGAAGAT+TGG - chr5_1:41100534-41100553 Msa0731030:CDS 40.0%
ATGATGCCATGAAGATTGGT+TGG - chr5_1:41100538-41100557 Msa0731030:CDS 40.0%
! TTGGTTGGAAATCTGGAATG+AGG - chr5_1:41100553-41100572 Msa0731030:CDS 40.0%
! TGGTTGGAAATCTGGAATGA+GGG - chr5_1:41100554-41100573 Msa0731030:CDS 40.0%
AGCGCATTTCTGCCATAGTT+TGG + chr5_1:41099815-41099834 None:intergenic 45.0%
AAACTATGGCAGAAATGCGC+TGG - chr5_1:41099814-41099833 Msa0731030:CDS 45.0%
CTACTTCCCACAAGGTCATT+TGG - chr5_1:41099876-41099895 Msa0731030:CDS 45.0%
GCATGTTCCAAATGACCTTG+TGG + chr5_1:41099886-41099905 None:intergenic 45.0%
TGGTGATTGTGGCAGCATTA+GGG + chr5_1:41099918-41099937 None:intergenic 45.0%
ATGGTGATTGTGGCAGCATT+AGG + chr5_1:41099919-41099938 None:intergenic 45.0%
GCCACAATCACCATTCTTGA+TGG - chr5_1:41099925-41099944 Msa0731030:CDS 45.0%
TGGACGGTAACCATCAAGAA+TGG + chr5_1:41099938-41099957 None:intergenic 45.0%
AGTAGAGACGATGCATGGAA+TGG + chr5_1:41099962-41099981 None:intergenic 45.0%
AGGAGGTTCTTGGTCTGTTA+CGG + chr5_1:41100043-41100062 None:intergenic 45.0%
TGTTTCCATCGTCGTCTTCT+TGG + chr5_1:41100083-41100102 None:intergenic 45.0%
! ATCAAGTCTGTGCATTGACG+AGG + chr5_1:41100165-41100184 None:intergenic 45.0%
TCTTGAGACTTCTGCAACTC+TGG + chr5_1:41100192-41100211 None:intergenic 45.0%
GTATAGGTGTAGACTTTGCC+TGG + chr5_1:41100234-41100253 None:intergenic 45.0%
! ATTAACTGAGAGTGCGGTCT+TGG - chr5_1:41100416-41100435 Msa0731030:CDS 45.0%
! TTAACTGAGAGTGCGGTCTT+GGG - chr5_1:41100417-41100436 Msa0731030:CDS 45.0%
GGATTTGGCTAACAAGAACG+TGG - chr5_1:41100446-41100465 Msa0731030:CDS 45.0%
CATGGCATCATCTACAACCT+TGG + chr5_1:41100529-41100548 None:intergenic 45.0%
! TATAAGAGGTTTTCAGCGGG+AGG - chr5_1:41100617-41100636 Msa0731030:CDS 45.0%
! ATAAGAGGTTTTCAGCGGGA+GGG - chr5_1:41100618-41100637 Msa0731030:CDS 45.0%
CCTTAAGCATGCGCCAATTA+GGG + chr5_1:41100671-41100690 None:intergenic 45.0%
ACCTTAAGCATGCGCCAATT+AGG + chr5_1:41100672-41100691 None:intergenic 45.0%
AGCGCATTTCTGCCATAGTT+TGG + chr5_1:41099815-41099834 None:intergenic 45.0%
AAACTATGGCAGAAATGCGC+TGG - chr5_1:41099814-41099833 Msa0731030:CDS 45.0%
CTACTTCCCACAAGGTCATT+TGG - chr5_1:41099876-41099895 Msa0731030:CDS 45.0%
GCATGTTCCAAATGACCTTG+TGG + chr5_1:41099886-41099905 None:intergenic 45.0%
TGGTGATTGTGGCAGCATTA+GGG + chr5_1:41099918-41099937 None:intergenic 45.0%
ATGGTGATTGTGGCAGCATT+AGG + chr5_1:41099919-41099938 None:intergenic 45.0%
GCCACAATCACCATTCTTGA+TGG - chr5_1:41099925-41099944 Msa0731030:CDS 45.0%
TGGACGGTAACCATCAAGAA+TGG + chr5_1:41099938-41099957 None:intergenic 45.0%
AGTAGAGACGATGCATGGAA+TGG + chr5_1:41099962-41099981 None:intergenic 45.0%
AGGAGGTTCTTGGTCTGTTA+CGG + chr5_1:41100043-41100062 None:intergenic 45.0%
TGTTTCCATCGTCGTCTTCT+TGG + chr5_1:41100083-41100102 None:intergenic 45.0%
! ATCAAGTCTGTGCATTGACG+AGG + chr5_1:41100165-41100184 None:intergenic 45.0%
TCTTGAGACTTCTGCAACTC+TGG + chr5_1:41100192-41100211 None:intergenic 45.0%
GTATAGGTGTAGACTTTGCC+TGG + chr5_1:41100234-41100253 None:intergenic 45.0%
! ATTAACTGAGAGTGCGGTCT+TGG - chr5_1:41100416-41100435 Msa0731030:CDS 45.0%
! TTAACTGAGAGTGCGGTCTT+GGG - chr5_1:41100417-41100436 Msa0731030:CDS 45.0%
GGATTTGGCTAACAAGAACG+TGG - chr5_1:41100446-41100465 Msa0731030:CDS 45.0%
CATGGCATCATCTACAACCT+TGG + chr5_1:41100529-41100548 None:intergenic 45.0%
! TATAAGAGGTTTTCAGCGGG+AGG - chr5_1:41100617-41100636 Msa0731030:CDS 45.0%
! ATAAGAGGTTTTCAGCGGGA+GGG - chr5_1:41100618-41100637 Msa0731030:CDS 45.0%
CCTTAAGCATGCGCCAATTA+GGG + chr5_1:41100671-41100690 None:intergenic 45.0%
ACCTTAAGCATGCGCCAATT+AGG + chr5_1:41100672-41100691 None:intergenic 45.0%
GGTGATTGTGGCAGCATTAG+GGG + chr5_1:41099917-41099936 None:intergenic 50.0%
CGATGCATGGAATGGATGGA+CGG + chr5_1:41099954-41099973 None:intergenic 50.0%
GAGACGATGCATGGAATGGA+TGG + chr5_1:41099958-41099977 None:intergenic 50.0%
CCAACAGTAGAGACGATGCA+TGG + chr5_1:41099967-41099986 None:intergenic 50.0%
CCATGCATCGTCTCTACTGT+TGG - chr5_1:41099964-41099983 Msa0731030:CDS 50.0%
CAGTTTGGCAAAGACCTCGT+CGG + chr5_1:41100010-41100029 None:intergenic 50.0%
TACGGGAGTTAGGAGCAGTT+TGG + chr5_1:41100025-41100044 None:intergenic 50.0%
CTTGGTCTGTTACGGGAGTT+AGG + chr5_1:41100035-41100054 None:intergenic 50.0%
GGAGGTTCTTGGTCTGTTAC+GGG + chr5_1:41100042-41100061 None:intergenic 50.0%
AGGTGTAGACTTTGCCTGGA+AGG + chr5_1:41100230-41100249 None:intergenic 50.0%
AACTTCCCTGCAGCTGAAAG+AGG - chr5_1:41100387-41100406 Msa0731030:CDS 50.0%
TCTCACCTCTTTCAGCTGCA+GGG + chr5_1:41100395-41100414 None:intergenic 50.0%
TTCTCACCTCTTTCAGCTGC+AGG + chr5_1:41100396-41100415 None:intergenic 50.0%
GAGGGCAAAGCTTCGTTTGA+TGG - chr5_1:41100572-41100591 Msa0731030:CDS 50.0%
! TAAGAGGTTTTCAGCGGGAG+GGG - chr5_1:41100619-41100638 Msa0731030:CDS 50.0%
CCCTAATTGGCGCATGCTTA+AGG - chr5_1:41100668-41100687 Msa0731030:CDS 50.0%
GGTGATTGTGGCAGCATTAG+GGG + chr5_1:41099917-41099936 None:intergenic 50.0%
CGATGCATGGAATGGATGGA+CGG + chr5_1:41099954-41099973 None:intergenic 50.0%
GAGACGATGCATGGAATGGA+TGG + chr5_1:41099958-41099977 None:intergenic 50.0%
CCAACAGTAGAGACGATGCA+TGG + chr5_1:41099967-41099986 None:intergenic 50.0%
CCATGCATCGTCTCTACTGT+TGG - chr5_1:41099964-41099983 Msa0731030:CDS 50.0%
CAGTTTGGCAAAGACCTCGT+CGG + chr5_1:41100010-41100029 None:intergenic 50.0%
TACGGGAGTTAGGAGCAGTT+TGG + chr5_1:41100025-41100044 None:intergenic 50.0%
CTTGGTCTGTTACGGGAGTT+AGG + chr5_1:41100035-41100054 None:intergenic 50.0%
GGAGGTTCTTGGTCTGTTAC+GGG + chr5_1:41100042-41100061 None:intergenic 50.0%
AGGTGTAGACTTTGCCTGGA+AGG + chr5_1:41100230-41100249 None:intergenic 50.0%
AACTTCCCTGCAGCTGAAAG+AGG - chr5_1:41100387-41100406 Msa0731030:CDS 50.0%
TCTCACCTCTTTCAGCTGCA+GGG + chr5_1:41100395-41100414 None:intergenic 50.0%
TTCTCACCTCTTTCAGCTGC+AGG + chr5_1:41100396-41100415 None:intergenic 50.0%
GAGGGCAAAGCTTCGTTTGA+TGG - chr5_1:41100572-41100591 Msa0731030:CDS 50.0%
! TAAGAGGTTTTCAGCGGGAG+GGG - chr5_1:41100619-41100638 Msa0731030:CDS 50.0%
CCCTAATTGGCGCATGCTTA+AGG - chr5_1:41100668-41100687 Msa0731030:CDS 50.0%
TGTTGATCCTCACACCGACG+AGG - chr5_1:41099993-41100012 Msa0731030:CDS 55.0%
TCTTCTTGGCCGTGAACCAC+AGG + chr5_1:41100069-41100088 None:intergenic 55.0%
GTCTCTGTCACTGACCTTCC+AGG - chr5_1:41100213-41100232 Msa0731030:CDS 55.0%
! GGTCTTGGGTGCAGTGGATT+TGG - chr5_1:41100431-41100450 Msa0731030:CDS 55.0%
TGTTGATCCTCACACCGACG+AGG - chr5_1:41099993-41100012 Msa0731030:CDS 55.0%
TCTTCTTGGCCGTGAACCAC+AGG + chr5_1:41100069-41100088 None:intergenic 55.0%
GTCTCTGTCACTGACCTTCC+AGG - chr5_1:41100213-41100232 Msa0731030:CDS 55.0%
! GGTCTTGGGTGCAGTGGATT+TGG - chr5_1:41100431-41100450 Msa0731030:CDS 55.0%
GCAAAGACCTCGTCGGTGTG+AGG + chr5_1:41100003-41100022 None:intergenic 60.0%
CCACAGGAGGAGGAGGTTCT+TGG + chr5_1:41100053-41100072 None:intergenic 60.0%
CCAAGAACCTCCTCCTCCTG+TGG - chr5_1:41100050-41100069 Msa0731030:CDS 60.0%
CCTCCTCCTCCTGTGGTTCA+CGG - chr5_1:41100057-41100076 Msa0731030:CDS 60.0%
TCTTGGCCGTGAACCACAGG+AGG + chr5_1:41100066-41100085 None:intergenic 60.0%
CACGGCCAAGAAGACGACGA+TGG - chr5_1:41100075-41100094 Msa0731030:CDS 60.0%
GCAAAGACCTCGTCGGTGTG+AGG + chr5_1:41100003-41100022 None:intergenic 60.0%
CCACAGGAGGAGGAGGTTCT+TGG + chr5_1:41100053-41100072 None:intergenic 60.0%
CCAAGAACCTCCTCCTCCTG+TGG - chr5_1:41100050-41100069 Msa0731030:CDS 60.0%
CCTCCTCCTCCTGTGGTTCA+CGG - chr5_1:41100057-41100076 Msa0731030:CDS 60.0%
TCTTGGCCGTGAACCACAGG+AGG + chr5_1:41100066-41100085 None:intergenic 60.0%
CACGGCCAAGAAGACGACGA+TGG - chr5_1:41100075-41100094 Msa0731030:CDS 60.0%
CCGTGAACCACAGGAGGAGG+AGG + chr5_1:41100060-41100079 None:intergenic 65.0%
TGGCCGTGAACCACAGGAGG+AGG + chr5_1:41100063-41100082 None:intergenic 65.0%
GAGTGCGGTCTTGGGTGCAG+TGG - chr5_1:41100425-41100444 Msa0731030:CDS 65.0%
CCGTGAACCACAGGAGGAGG+AGG + chr5_1:41100060-41100079 None:intergenic 65.0%
TGGCCGTGAACCACAGGAGG+AGG + chr5_1:41100063-41100082 None:intergenic 65.0%
GAGTGCGGTCTTGGGTGCAG+TGG - chr5_1:41100425-41100444 Msa0731030:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 41099805 41100758 41099805 ID=Msa0731030;Name=Msa0731030
chr5_1 mRNA 41099805 41100758 41099805 ID=Msa0731030-mRNA-1;Parent=Msa0731030;Name=Msa0731030-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|-1|0|1|-1|1|1|0|317
chr5_1 exon 41099805 41100758 41099805 ID=Msa0731030-mRNA-1:exon:11999;Parent=Msa0731030-mRNA-1
chr5_1 CDS 41099805 41100758 41099805 ID=Msa0731030-mRNA-1:cds;Parent=Msa0731030-mRNA-1
Gene Sequence

>Msa0731030

ATGGACTCCAAACTATGGCAGAAATGCGCTGGTCCCGACGTCAAGATTCCAAAGATTCACTCTAAAGTTTACTACTTCCCACAAGGTCATTTGGAACATGCATGTTCTTCCCCTAATGCTGCCACAATCACCATTCTTGATGGTTACCGTCCATCCATTCCATGCATCGTCTCTACTGTTGGTCTCTTTGTTGATCCTCACACCGACGAGGTCTTTGCCAAACTGCTCCTAACTCCCGTAACAGACCAAGAACCTCCTCCTCCTGTGGTTCACGGCCAAGAAGACGACGATGGAAACAATCTTGTTTCCTATGTCAAGACTCTCACACTAACTGATACTAAATCTGGATTCAATATGCCTCGTCAATGCACAGACTTGATCTTTCCAGAGTTGCAGAAGTCTCAAGAAGTCTCTGTCACTGACCTTCCAGGCAAAGTCTACACCTATACCTACTCAAAACAGAGTAGACTAACTAAAGGATGGTTTAATTTTGTAAGTGAGAAGAAACTCGTCCCCGAAGATTCCGTTGTTTTCATCAAAAACTCTGCCGGAAAAATATATGTCGGAATTCGCAGGAACACTAACTTCCCTGCAGCTGAAAGAGGTGAGAAATTAACTGAGAGTGCGGTCTTGGGTGCAGTGGATTTGGCTAACAAGAACGTGGCGTTTGAAGTTGTGTTTTATCCCACAGATGGGAATTGGTGTAATTTTGTAGTGGATGCCAAGGTTGTAGATGATGCCATGAAGATTGGTTGGAAATCTGGAATGAGGGCAAAGCTTCGTTTGATGGAACCTGAATCTTCAAATTTAACTATAAGAGGTTTTCAGCGGGAGGGGACAATCTCTAATGATTCTTCTAATGTCCCTAATTGGCGCATGCTTAAGGTACTTATGCTTTCTTATTTTGTTCTAATGGTATTTCAATTGCATATTGTTATCAAGTTGAATCCATAA
Protein sequence

>Msa0731030

MDSKLWQKCAGPDVKIPKIHSKVYYFPQGHLEHACSSPNAATITILDGYRPSIPCIVSTVGLFVDPHTDEVFAKLLLTPVTDQEPPPPVVHGQEDDDGNNLVSYVKTLTLTDTKSGFNMPRQCTDLIFPELQKSQEVSVTDLPGKVYTYTYSKQSRLTKGWFNFVSEKKLVPEDSVVFIKNSAGKIYVGIRRNTNFPAAERGEKLTESAVLGAVDLANKNVAFEVVFYPTDGNWCNFVVDAKVVDDAMKIGWKSGMRAKLRLMEPESSNLTIRGFQREGTISNDSSNVPNWRMLKVLMLSYFVLMVFQLHIVIKLNP*