AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0731100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0731100 MtrunA17_Chr5g0424931 92.614 528 38 1 1 527 1 528 0.0 1004
Msa0731100 MtrunA17_Chr1g0178011 54.598 522 160 8 8 525 1 449 4.18e-176 504
Msa0731100 MtrunA17_Chr5g0417281 49.817 546 207 11 14 527 6 516 1.26e-158 462
Msa0731100 MtrunA17_Chr5g0436761 48.362 519 191 9 14 521 7 459 4.26e-151 440
Msa0731100 MtrunA17_Chr5g0424741 47.426 544 188 13 8 479 1 518 1.01e-142 421
Msa0731100 MtrunA17_Chr3g0115271 45.191 551 248 15 19 526 19 558 1.14e-142 423
Msa0731100 MtrunA17_Chr2g0277461 48.162 517 184 14 15 523 9 449 3.66e-136 402
Msa0731100 MtrunA17_Chr5g0424801 53.299 394 139 10 8 391 1 359 4.37e-129 381
Msa0731100 MtrunA17_Chr5g0425261 53.708 391 142 9 8 391 1 359 4.04e-128 379
Msa0731100 MtrunA17_Chr5g0417211 42.857 525 161 12 15 526 8 406 6.42e-117 351
Msa0731100 MtrunA17_Chr2g0289221 54.000 350 117 9 6 346 7 321 6.53e-117 349
Msa0731100 MtrunA17_Chr2g0326761 39.448 507 240 15 19 477 11 498 5.00e-100 317
Msa0731100 MtrunA17_Chr1g0181211 40.671 477 221 12 19 451 27 485 4.28e-99 314
Msa0731100 MtrunA17_Chr7g0264171 38.655 476 217 13 14 437 26 478 2.55e-97 307
Msa0731100 MtrunA17_Chr4g0028971 41.150 452 212 12 19 431 12 448 5.46e-93 298
Msa0731100 MtrunA17_Chr1g0199681 40.889 450 216 14 19 431 94 530 1.32e-92 297
Msa0731100 MtrunA17_Chr2g0277531 47.059 340 127 12 19 346 20 318 3.88e-87 271
Msa0731100 MtrunA17_Chr5g0425661 62.051 195 66 3 19 208 37 228 3.30e-78 245
Msa0731100 MtrunA17_Chr7g0264161 40.909 330 153 9 29 346 2 301 4.18e-72 231
Msa0731100 MtrunA17_Chr2g0282961 33.333 387 200 9 1 366 38 387 1.18e-65 224
Msa0731100 MtrunA17_Chr2g0285461 35.500 400 208 12 6 384 6 376 1.63e-60 214
Msa0731100 MtrunA17_Chr2g0276781 35.450 378 207 7 19 385 23 374 6.24e-60 209
Msa0731100 MtrunA17_Chr4g0046371 35.027 374 188 10 16 366 35 376 7.99e-60 209
Msa0731100 MtrunA17_Chr8g0385791 33.943 383 207 9 19 385 59 411 3.90e-59 209
Msa0731100 MtrunA17_Chr4g0070181 34.140 372 196 7 14 368 18 357 6.39e-59 211
Msa0731100 MtrunA17_Chr5g0433301 34.974 386 199 11 19 384 24 377 7.18e-59 209
Msa0731100 MtrunA17_Chr3g0109391 33.766 385 205 9 19 384 26 379 1.72e-58 208
Msa0731100 MtrunA17_Chr4g0009751 35.171 381 205 10 19 385 19 371 1.81e-58 204
Msa0731100 MtrunA17_Chr2g0301251 34.146 369 200 7 14 368 18 357 5.86e-58 208
Msa0731100 MtrunA17_Chr1g0156321 35.262 363 191 9 19 366 43 376 2.58e-57 205
Msa0731100 MtrunA17_Chr8g0376771 33.915 401 212 12 6 384 6 375 7.76e-57 204
Msa0731100 MtrunA17_Chr8g0389081 34.239 368 201 9 16 369 29 369 1.33e-55 201
Msa0731100 MtrunA17_Chr7g0239061 31.626 449 260 14 19 448 14 434 1.86e-55 197
Msa0731100 MtrunA17_Chr2g0326281 32.346 405 209 12 18 388 55 428 1.31e-54 197
Msa0731100 MtrunA17_Chr4g0029671 33.417 398 201 10 18 385 53 416 4.76e-53 192
Msa0731100 MtrunA17_Chr8g0346711 33.598 378 211 9 19 385 29 377 2.02e-52 189
Msa0731100 MtrunA17_Chr5g0425651 72.321 112 27 1 263 370 1 112 1.36e-49 166
Msa0731100 MtrunA17_Chr5g0424791 46.667 150 66 4 68 213 12 151 7.19e-32 120
Msa0731100 MtrunA17_Chr8g0356211 26.446 242 155 3 125 366 2 220 9.53e-23 99.0
Msa0731100 MtrunA17_Chr5g0419861 39.130 115 57 4 57 163 12 121 1.23e-13 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0731100 AT1G77850.1 38.783 575 285 14 1 526 1 557 7.17e-121 367
Msa0731100 AT1G77850.2 38.783 575 285 14 1 526 1 557 9.87e-121 367
Msa0731100 AT4G30080.1 39.279 555 272 21 19 526 19 555 2.26e-95 304
Msa0731100 AT2G28350.2 42.327 404 190 11 19 388 10 404 3.11e-87 283
Msa0731100 AT2G28350.1 42.327 404 190 11 19 388 10 404 3.48e-87 283
Msa0731100 AT2G46530.1 36.769 359 193 8 19 369 21 353 3.27e-63 217
Msa0731100 AT2G46530.3 36.798 356 191 8 19 366 42 371 5.53e-63 217
Msa0731100 AT2G33860.1 34.865 370 190 8 16 366 51 388 1.08e-62 216
Msa0731100 AT5G37020.2 34.000 400 213 11 19 399 22 389 3.12e-61 215
Msa0731100 AT5G37020.1 34.000 400 213 11 19 399 22 389 5.84e-61 215
Msa0731100 AT3G61830.1 35.040 371 197 9 19 381 25 359 1.86e-60 210
Msa0731100 AT1G30330.3 34.197 386 212 8 14 384 20 378 2.01e-60 214
Msa0731100 AT1G30330.2 34.197 386 212 8 14 384 20 378 2.01e-60 214
Msa0731100 AT1G30330.1 34.197 386 212 8 14 384 18 376 2.04e-60 214
Msa0731100 AT4G23980.2 30.103 485 284 13 19 483 12 461 3.55e-57 201
Msa0731100 AT5G60450.2 34.271 391 203 13 13 382 35 392 4.77e-57 203
Msa0731100 AT5G62000.4 32.921 404 215 10 3 385 45 413 6.83e-57 204
Msa0731100 AT4G23980.1 33.333 384 214 9 19 389 12 366 8.01e-57 201
Msa0731100 AT5G62000.2 32.921 404 215 10 3 385 45 413 9.26e-57 204
Msa0731100 AT5G62000.5 32.921 404 215 10 3 385 45 413 9.26e-57 204
Msa0731100 AT5G62000.3 32.921 404 215 10 3 385 45 413 9.26e-57 204
Msa0731100 AT5G62000.1 32.921 404 215 10 3 385 45 413 9.26e-57 204
Msa0731100 AT5G60450.1 34.545 385 198 13 19 382 65 416 1.08e-56 202
Msa0731100 AT5G20730.2 33.957 374 194 8 14 368 19 358 1.49e-56 204
Msa0731100 AT1G59750.4 33.884 363 204 6 19 371 22 358 1.84e-56 200
Msa0731100 AT1G59750.2 33.884 363 204 6 19 371 22 358 1.88e-56 200
Msa0731100 AT1G59750.1 33.884 363 204 6 19 371 22 358 2.40e-56 200
Msa0731100 AT1G59750.3 33.884 363 204 6 19 371 22 358 2.40e-56 200
Msa0731100 AT1G19220.1 33.850 387 208 11 16 385 20 375 1.35e-55 201
Msa0731100 AT1G34310.1 33.508 382 211 9 19 389 27 376 1.56e-55 196
Msa0731100 AT5G20730.3 33.867 375 194 9 14 368 19 359 4.89e-55 200
Msa0731100 AT5G20730.1 33.867 375 194 9 14 368 19 359 5.12e-55 200
Msa0731100 AT1G35520.1 34.204 383 207 11 19 389 27 376 6.48e-55 194
Msa0731100 AT1G34410.1 32.323 396 226 10 19 405 27 389 9.74e-55 194
Msa0731100 AT1G34390.1 32.558 387 214 10 19 397 27 374 1.60e-54 194
Msa0731100 AT1G19850.2 34.153 366 198 9 16 366 40 377 4.79e-54 196
Msa0731100 AT1G19850.1 34.153 366 198 9 16 366 51 388 6.69e-54 196
Msa0731100 AT1G35240.1 32.741 394 213 12 19 398 27 382 5.89e-52 186
Msa0731100 AT1G35540.1 32.105 380 219 9 19 389 27 376 6.85e-52 186
Msa0731100 AT1G34170.1 31.421 366 195 10 19 366 25 352 8.10e-47 171
Msa0731100 AT3G61830.2 34.036 332 179 8 54 381 13 308 1.19e-46 171
Msa0731100 AT2G33860.2 34.241 257 135 4 117 366 2 231 3.65e-45 165
Msa0731100 AT2G46530.2 36.965 257 137 4 114 369 34 266 6.93e-44 163
Msa0731100 AT5G37020.3 34.589 292 154 8 117 399 31 294 3.89e-42 160
Msa0731100 AT1G34170.3 27.115 520 269 17 19 507 25 465 3.46e-41 156
Msa0731100 AT1G34170.2 29.781 366 175 10 19 366 25 326 1.90e-40 152
Msa0731100 AT1G43950.1 38.725 204 111 5 19 216 27 222 1.27e-37 138
Msa0731100 ATMG00940.1 34.586 133 75 4 204 327 1 130 6.76e-15 73.2

Find 150 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGGTTCAATTAAGTTATT+TGG 0.154755 5_1:-41307401 Msa0731100:CDS
TTCAAACTACTAAGGTTATT+TGG 0.200926 5_1:+41307479 None:intergenic
TCTCTTTATCATAGGTTAAC+TGG 0.215844 5_1:-41307856 Msa0731100:intron
CCCTAATTGGCGCATGCTTC+AGG 0.273598 5_1:-41309626 Msa0731100:intron
AGAGAATGGTTTGGAGGTTA+AGG 0.274717 5_1:-41309929 Msa0731100:CDS
CATGATCATTTATCTCTATC+AGG 0.276134 5_1:-41307587 Msa0731100:CDS
TGAACCAAACATTGTTGAAT+TGG 0.283162 5_1:-41307643 Msa0731100:CDS
TATATTACCCTAGGGCTAAT+TGG 0.305719 5_1:-41309807 Msa0731100:CDS
AATTTCATTGGATCCTTTAG+AGG 0.319231 5_1:+41307319 None:intergenic
CAAACAATCGCCGTTATTGA+TGG 0.325853 5_1:-41310474 Msa0731100:CDS
GTCAATGCATCAAATTTCAT+TGG 0.331745 5_1:+41307307 None:intergenic
CTGATGTTCATGACGGAGTT+TGG 0.334825 5_1:-41310161 Msa0731100:CDS
TACCGGACTGAGTAGCAATT+TGG 0.335469 5_1:+41310377 None:intergenic
AGCCCCAATTCAACAATGTT+TGG 0.335857 5_1:+41307639 None:intergenic
ACGGCGATTGTTTGAGCGTT+AGG 0.342143 5_1:+41310483 None:intergenic
ATGAAGATTGGTTGGGCATC+TGG 0.343084 5_1:-41309745 Msa0731100:CDS
ACCTGAAGCATGCGCCAATT+AGG 0.343626 5_1:+41309625 None:intergenic
GAACCAAACATTGTTGAATT+GGG 0.349855 5_1:-41307642 Msa0731100:CDS
CTTAGTAGTTTGAACACATT+CGG 0.352705 5_1:-41307470 Msa0731100:CDS
GAGAATGGTTTGGAGGTTAA+GGG 0.354789 5_1:-41309928 Msa0731100:CDS
GAAAGTGATGTCCATGATTC+TGG 0.371511 5_1:-41307359 Msa0731100:CDS
GGAGAATTTAAGTCAAGCTT+GGG 0.377595 5_1:+41310210 None:intergenic
CCTGAAGCATGCGCCAATTA+GGG 0.379672 5_1:+41309626 None:intergenic
TAAACTTTAGAACGTACCTT+TGG 0.384455 5_1:+41310546 None:intergenic
TGCCTCAATAACCGCCTTCT+CGG 0.388165 5_1:+41309867 None:intergenic
GAGTCCAACCAGTAGTAAAA+AGG 0.388502 5_1:+41310100 None:intergenic
TCTTTGAATGATAATAGTTA+TGG 0.390167 5_1:-41307554 Msa0731100:CDS
CGGCGATTGTTTGAGCGTTA+GGG 0.395501 5_1:+41310484 None:intergenic
GGAGTTGGCGGAAAAGAATA+TGG 0.402969 5_1:-41309842 Msa0731100:CDS
CTCTTTATCATAGGTTAACT+GGG 0.403608 5_1:-41307855 Msa0731100:intron
GGGACAGACATGGTGGCCGG+TGG 0.403787 5_1:+41310630 None:intergenic
TAGATGCACTGCAGGATTGC+GGG 0.405787 5_1:+41307772 None:intergenic
CAGCAGGCATGCAGGGAGTC+AGG 0.416086 5_1:-41307610 Msa0731100:CDS
TTGAATTTCACGCGGCGGGC+CGG 0.416798 5_1:+41310606 None:intergenic
AGTTCTTGTCCTACAGTTAC+CGG 0.417820 5_1:+41310360 None:intergenic
GCCGCGTGAAATTCAAAATC+TGG 0.417967 5_1:-41310599 Msa0731100:CDS
ATGTGTCCAGTGTCCCTAAT+TGG 0.419261 5_1:-41309639 Msa0731100:CDS
TTAGTAGTTTGAACACATTC+GGG 0.421729 5_1:-41307469 Msa0731100:CDS
ACTATTATCATTCAAAGAGT+TGG 0.428670 5_1:+41307559 None:intergenic
ATCAAAACCGCGTCAATCCT+TGG 0.437926 5_1:-41307811 Msa0731100:CDS
ATCAACGGCCGTGATGATAC+AGG 0.446735 5_1:+41310434 None:intergenic
TTGAAGTTATATATTACCCT+AGG 0.447607 5_1:-41309816 Msa0731100:CDS
AGGAGAATTTAAGTCAAGCT+TGG 0.457295 5_1:+41310209 None:intergenic
CGCCAAATTGCTACTCAGTC+CGG 0.457882 5_1:-41310379 Msa0731100:CDS
CTGACTCCCTGCATGCCTGC+TGG 0.459021 5_1:+41307611 None:intergenic
ACAAAATCTGATTCTAATAA+CGG 0.462788 5_1:-41310276 Msa0731100:CDS
AAGGAGGAGGGTAAAGAGAA+TGG 0.463485 5_1:-41309943 Msa0731100:CDS
GAACTCTATCATAGGAATGA+AGG 0.464627 5_1:+41307679 None:intergenic
GGTTTGGAGGTTAAGGGAGA+TGG 0.469959 5_1:-41309922 Msa0731100:CDS
TCTTCTTCATCAACAACCTC+GGG 0.472000 5_1:+41310336 None:intergenic
CTCTGAGGGAACTCTATCAT+AGG 0.472447 5_1:+41307671 None:intergenic
AACACAAAAGGAGTTGGTTC+TGG 0.474124 5_1:-41307419 Msa0731100:CDS
GGAGGGTAAAGAGAATGGTT+TGG 0.480150 5_1:-41309938 Msa0731100:CDS
AATTTCACGCGGCGGGCCGG+AGG 0.480942 5_1:+41310609 None:intergenic
GGCCACCATGTCTGTCCCTC+CGG 0.483118 5_1:-41310625 Msa0731100:CDS
GTGGATGATGCTATGAAGAT+TGG 0.483610 5_1:-41309757 Msa0731100:CDS
AAGGGTGAAGATTCCTCTAA+AGG 0.485287 5_1:-41307332 Msa0731100:CDS
TTTAGAGGAATCTTCACCCT+TGG 0.485917 5_1:+41307334 None:intergenic
GGTTATTGAGGCAGTGGAGT+TGG 0.491818 5_1:-41309857 Msa0731100:CDS
TTACTTCCCACAAGGTCACT+TGG 0.494429 5_1:-41310523 Msa0731100:CDS
TGGAACGTTCACCGATGGAC+CGG 0.495600 5_1:+41310566 None:intergenic
CGAATGTGTTCAAACTACTA+AGG 0.499098 5_1:+41307471 None:intergenic
ACCTTTGGAACGTTCACCGA+TGG 0.510835 5_1:+41310561 None:intergenic
TTCTTCTTCATCAACAACCT+CGG 0.514354 5_1:+41310335 None:intergenic
AAAATCTGGCAGACATGTGC+CGG 0.515291 5_1:-41310585 Msa0731100:CDS
GGACAGACATGGTGGCCGGT+GGG 0.516458 5_1:+41310631 None:intergenic
GCAGACATGTGCCGGTCCAT+CGG 0.518088 5_1:-41310577 Msa0731100:CDS
GTGAATAATAAGAAGGAGGA+GGG 0.519419 5_1:-41309955 Msa0731100:CDS
AAAAGCAGTGAATAATAAGA+AGG 0.519919 5_1:-41309962 Msa0731100:CDS
ATTGTTTGAGCGTTAGGGGA+AGG 0.521449 5_1:+41310489 None:intergenic
ACTGCCGGAGATATAGTTGT+TGG 0.521728 5_1:-41310021 Msa0731100:CDS
AACCGAGAAGGCGGTTATTG+AGG 0.522496 5_1:-41309869 Msa0731100:CDS
ACTCACAACAACAACACAAA+AGG 0.522870 5_1:-41307431 Msa0731100:CDS
TCTGATTCTAATAACGGCGG+TGG 0.523119 5_1:-41310270 Msa0731100:CDS
GAAGATTCATCAATCTTCAA+AGG 0.524456 5_1:+41309706 None:intergenic
GTAGAATCCTGAACTAGTCT+AGG 0.524565 5_1:+41307728 None:intergenic
AGTGAATAATAAGAAGGAGG+AGG 0.524591 5_1:-41309956 Msa0731100:CDS
GAGAGTTCCTGAGACGGAAA+AGG 0.524822 5_1:+41310189 None:intergenic
GACAGACATGGTGGCCGGTG+GGG 0.526151 5_1:+41310632 None:intergenic
GGGTAAAGAGAATGGTTTGG+AGG 0.526164 5_1:-41309935 Msa0731100:CDS
CACCCTTGGTACCAGAATCA+TGG 0.526655 5_1:+41307348 None:intergenic
CAGCAAACTTCATGTTTCTG+CGG 0.530328 5_1:+41309995 None:intergenic
GGTGGCCGGTGGGGAAGACA+TGG 0.530680 5_1:+41310641 None:intergenic
GACCTCATCGGTGTGAGGAT+CGG 0.532501 5_1:+41310404 None:intergenic
TGTAGTTGATTCAAATGTTG+TGG 0.533977 5_1:-41309776 Msa0731100:CDS
GGAGGGACAGACATGGTGGC+CGG 0.536529 5_1:+41310627 None:intergenic
AACCAAACATTGTTGAATTG+GGG 0.537810 5_1:-41307641 Msa0731100:CDS
AACTCTATCATAGGAATGAA+GGG 0.541293 5_1:+41307680 None:intergenic
TTCCTTTCCCTGTATCATCA+CGG 0.541294 5_1:-41310442 Msa0731100:CDS
AGAAATGGTAGATGCACTGC+AGG 0.541883 5_1:+41307764 None:intergenic
GATTCCAACAACTATATCTC+CGG 0.544415 5_1:+41310017 None:intergenic
CATGTTCCAAGTGACCTTGT+GGG 0.545385 5_1:+41310517 None:intergenic
TTGTTTGAGCGTTAGGGGAA+GGG 0.546169 5_1:+41310490 None:intergenic
TACCTTGTTTAGTGATAACA+AGG 0.546334 5_1:-41307708 Msa0731100:CDS
TATTGAGGCAGTGGAGTTGG+CGG 0.551866 5_1:-41309854 Msa0731100:CDS
GAAATAAGTTCAACTTGCCA+AGG 0.552664 5_1:+41307794 None:intergenic
GAAGGCGGTTATTGAGGCAG+TGG 0.558765 5_1:-41309863 Msa0731100:CDS
TTGAGCGTTAGGGGAAGGGC+AGG 0.559568 5_1:+41310494 None:intergenic
ATGATGCTATGAAGATTGGT+TGG 0.562627 5_1:-41309753 Msa0731100:CDS
TTTGGTTCAAAGATCTCTGA+GGG 0.562864 5_1:+41307657 None:intergenic
TGAAGTTATATATTACCCTA+GGG 0.565837 5_1:-41309815 Msa0731100:CDS
TGGCGACAAGTCGTTAGATG+TGG 0.568778 5_1:+41307499 None:intergenic
GGCGATTGTTTGAGCGTTAG+GGG 0.568994 5_1:+41310485 None:intergenic
GCATGTTCCAAGTGACCTTG+TGG 0.569473 5_1:+41310516 None:intergenic
AAATTGCACCAATTAGCCCT+AGG 0.571867 5_1:+41309799 None:intergenic
ATCATGGACATCACTTTCAA+CGG 0.572291 5_1:+41307364 None:intergenic
TTCCACCATGTCTTCCCCAC+CGG 0.577652 5_1:-41310646 None:intergenic
TGATGCTATGAAGATTGGTT+GGG 0.580467 5_1:-41309752 Msa0731100:CDS
GGCCGTGATGATACAGGGAA+AGG 0.585344 5_1:+41310440 None:intergenic
ATTTCACGCGGCGGGCCGGA+GGG 0.586809 5_1:+41310610 None:intergenic
AAGGGGAATGCTAACCGAGA+AGG 0.592212 5_1:-41309881 Msa0731100:CDS
GTAGATGCACTGCAGGATTG+CGG 0.593419 5_1:+41307771 None:intergenic
GGCGACAAGTCGTTAGATGT+GGG 0.596585 5_1:+41307500 None:intergenic
AACAACAACACAAAAGGAGT+TGG 0.596832 5_1:-41307425 Msa0731100:CDS
TGTCCATGATTCTGGTACCA+AGG 0.599100 5_1:-41307351 Msa0731100:CDS
CATGCGCCAATTAGGGACAC+TGG 0.600814 5_1:+41309633 None:intergenic
CTACTCAGTCCGGTAACTGT+AGG 0.600878 5_1:-41310369 Msa0731100:CDS
GTGATGATACAGGGAAAGGA+AGG 0.601435 5_1:+41310444 None:intergenic
TCCATCGGTGAACGTTCCAA+AGG 0.605082 5_1:-41310562 Msa0731100:CDS
AAGTTTGCTCATGTCTATCG+AGG 0.613801 5_1:-41310138 Msa0731100:CDS
GTCCATGATTCTGGTACCAA+GGG 0.620974 5_1:-41307350 Msa0731100:CDS
TCAACGGCCGTGATGATACA+GGG 0.621862 5_1:+41310435 None:intergenic
GCGGGCCGGAGGGACAGACA+TGG 0.622803 5_1:+41310620 None:intergenic
AGGACGGTAACCATCAATAA+CGG 0.623715 5_1:+41310464 None:intergenic
ACCCTTGTTATCACTAAACA+AGG 0.625546 5_1:+41307706 None:intergenic
CAACTTGCCAAGGATTGACG+CGG 0.626664 5_1:+41307804 None:intergenic
AGCAGTGAATAATAAGAAGG+AGG 0.629221 5_1:-41309959 Msa0731100:CDS
CAATTTGGCGAAGACCTCAT+CGG 0.632488 5_1:+41310392 None:intergenic
AATTGCACCAATTAGCCCTA+GGG 0.639117 5_1:+41309800 None:intergenic
ATCAAGTCAGCACAAAGGCG+AGG 0.645484 5_1:+41310237 None:intergenic
GTTTGGTTCAAAGATCTCTG+AGG 0.646990 5_1:+41307656 None:intergenic
TCCGTCATGAACATCAGTAA+CGG 0.649838 5_1:+41310167 None:intergenic
GCGAAGACCTCATCGGTGTG+AGG 0.652471 5_1:+41310399 None:intergenic
GGGAATGCTAACCGAGAAGG+CGG 0.652518 5_1:-41309878 Msa0731100:CDS
AGTTTGCTCATGTCTATCGA+GGG 0.654001 5_1:-41310137 Msa0731100:CDS
TCCGTTACTGATGTTCATGA+CGG 0.654864 5_1:-41310168 Msa0731100:CDS
CATCGGTGTGAGGATCGGCT+AGG 0.655915 5_1:+41310409 None:intergenic
GTTGTTGATGAAGAAGAAGA+CGG 0.659219 5_1:-41310330 Msa0731100:CDS
AGCCGATCCTCACACCGATG+AGG 0.659256 5_1:-41310406 Msa0731100:CDS
GGCCGGAGGGACAGACATGG+TGG 0.661369 5_1:+41310623 None:intergenic
AAAGTTTATTACTTCCCACA+AGG 0.663461 5_1:-41310531 Msa0731100:CDS
ACATTAGAGATTGTTCCTTG+AGG 0.666803 5_1:+41309658 None:intergenic
TGATACAGGGAAAGGAAGGA+CGG 0.682674 5_1:+41310448 None:intergenic
GTAACGGAGAGTTCCTGAGA+CGG 0.687969 5_1:+41310183 None:intergenic
GAGAATTTAAGTCAAGCTTG+GGG 0.690174 5_1:+41310211 None:intergenic
AGGATCGGCTAGGAGATCAA+CGG 0.692255 5_1:+41310419 None:intergenic
AAATCTGATTCTAATAACGG+CGG 0.706742 5_1:-41310273 Msa0731100:CDS
GGAAGATCAAGTCAGCACAA+AGG 0.715164 5_1:+41310232 None:intergenic
ACCTTGTTTAGTGATAACAA+GGG 0.724822 5_1:-41307707 Msa0731100:CDS
TGTAGGACAAGAACTTCCCG+AGG 0.729698 5_1:-41310352 Msa0731100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTATTTTTTGAATCT+AGG - chr5_1:41308381-41308400 Msa0731100:intron 10.0%
!!! TATTTTATTTTTTGAATCTA+GGG - chr5_1:41308382-41308401 Msa0731100:intron 10.0%
!! CAATTTAGTATATAAATATT+AGG - chr5_1:41309409-41309428 Msa0731100:intron 10.0%
!!! AGAATACTTTTTTTTTTTTT+TGG - chr5_1:41309631-41309650 Msa0731100:CDS 10.0%
!!! TGCATTTTATTATAAAAATA+TGG + chr5_1:41309941-41309960 None:intergenic 10.0%
!! ATAGTTTAGTATACTTAATT+AGG + chr5_1:41308597-41308616 None:intergenic 15.0%
!!! ATTTTACATTAATAATTGCT+TGG + chr5_1:41309057-41309076 None:intergenic 15.0%
!!! AAAACATGATTTTTTAAACT+TGG + chr5_1:41309379-41309398 None:intergenic 15.0%
!! TTATATACTAAATTGATCAA+GGG + chr5_1:41309404-41309423 None:intergenic 15.0%
!! TTTATATACTAAATTGATCA+AGG + chr5_1:41309405-41309424 None:intergenic 15.0%
!!! CTATAATTATGGATTAATTT+TGG + chr5_1:41309721-41309740 None:intergenic 15.0%
!! ACAAAATCTGATTCTAATAA+CGG - chr5_1:41307679-41307698 Msa0731100:CDS 20.0%
!! TTGAAACTATAATCAAATGA+TGG - chr5_1:41309001-41309020 Msa0731100:intron 20.0%
!! AAATATTAGGGTTAAGTTAT+AGG - chr5_1:41309422-41309441 Msa0731100:intron 20.0%
!! TATTTATAACTTATCCGATA+GGG + chr5_1:41309477-41309496 None:intergenic 20.0%
!! TTATTTATAACTTATCCGAT+AGG + chr5_1:41309478-41309497 None:intergenic 20.0%
!!! GTCGTTAATTTACTATTTTT+TGG - chr5_1:41309528-41309547 Msa0731100:intron 20.0%
!!! TGGAAAAGTATTAGATATTT+AGG - chr5_1:41309912-41309931 Msa0731100:CDS 20.0%
!! CTATGATAAAGAGATAAAAA+TGG + chr5_1:41310093-41310112 None:intergenic 20.0%
!!! CATTTTTATCTCTTTATCAT+AGG - chr5_1:41310091-41310110 Msa0731100:CDS 20.0%
!! TCTTTGAATGATAATAGTTA+TGG - chr5_1:41310401-41310420 Msa0731100:CDS 20.0%
!! TTTTGTAAGAGTCTTTACAA+AGG + chr5_1:41307665-41307684 None:intergenic 25.0%
! AAAAGCAGTGAATAATAAGA+AGG - chr5_1:41307993-41308012 Msa0731100:intron 25.0%
! TTGAAGTTATATATTACCCT+AGG - chr5_1:41308139-41308158 Msa0731100:intron 25.0%
! TGAAGTTATATATTACCCTA+GGG - chr5_1:41308140-41308159 Msa0731100:intron 25.0%
! TATTATTGTCGAAGAAGTTT+AGG - chr5_1:41308436-41308455 Msa0731100:intron 25.0%
!! AGTGTAAAAAAAAGGTTTTC+GGG + chr5_1:41308681-41308700 None:intergenic 25.0%
!! GAGTGTAAAAAAAAGGTTTT+CGG + chr5_1:41308682-41308701 None:intergenic 25.0%
! GAAAAGTGAGTGTAAAAAAA+AGG + chr5_1:41308689-41308708 None:intergenic 25.0%
! TAAAGAGAAAGAAAAATCTC+CGG + chr5_1:41308808-41308827 None:intergenic 25.0%
!! ATTGCTTGGTATTAGAATTT+TGG + chr5_1:41309043-41309062 None:intergenic 25.0%
! AACATTATTCAACGATTATG+AGG - chr5_1:41309131-41309150 Msa0731100:intron 25.0%
! ATGATTACCAAAATACAGTT+AGG - chr5_1:41309155-41309174 Msa0731100:intron 25.0%
!! GATTTTTCCTAACTGTATTT+TGG + chr5_1:41309165-41309184 None:intergenic 25.0%
! GGTATATATGTTTAATCAAG+AGG - chr5_1:41309281-41309300 Msa0731100:intron 25.0%
! TATGTTTAATCAAGAGGAAT+AGG - chr5_1:41309287-41309306 Msa0731100:intron 25.0%
! ATGTTTAATCAAGAGGAATA+GGG - chr5_1:41309288-41309307 Msa0731100:intron 25.0%
! AAATTAACGACTAACTTAGT+TGG + chr5_1:41309519-41309538 None:intergenic 25.0%
! TTACCTTCAACACTAATAAA+AGG - chr5_1:41309563-41309582 Msa0731100:intron 25.0%
! TACCTTCAACACTAATAAAA+GGG - chr5_1:41309564-41309583 Msa0731100:intron 25.0%
! TTGCAGTTGATCTATAATTA+TGG + chr5_1:41309732-41309751 None:intergenic 25.0%
!!! GTTTTCAGGCATATAAATAA+TGG - chr5_1:41309869-41309888 Msa0731100:CDS 25.0%
! ATTTACTCTTATTCCTAAGT+AGG - chr5_1:41309960-41309979 Msa0731100:CDS 25.0%
! GTAAAATATAGATCCTACTT+AGG + chr5_1:41309976-41309995 None:intergenic 25.0%
! AGTATGTATATGCACATAAA+AGG + chr5_1:41310028-41310047 None:intergenic 25.0%
!!! ATATTTTGAATGACTGATGT+TGG - chr5_1:41310060-41310079 Msa0731100:CDS 25.0%
! ACTATTATCATTCAAAGAGT+TGG + chr5_1:41310399-41310418 None:intergenic 25.0%
! TTCAAACTACTAAGGTTATT+TGG + chr5_1:41310479-41310498 None:intergenic 25.0%
!! TCTGGTTCAATTAAGTTATT+TGG - chr5_1:41310554-41310573 Msa0731100:CDS 25.0%
!! TAAACTTTAGAACGTACCTT+TGG + chr5_1:41307412-41307431 None:intergenic 30.0%
AAAGTTTATTACTTCCCACA+AGG - chr5_1:41307424-41307443 Msa0731100:CDS 30.0%
AAATCTGATTCTAATAACGG+CGG - chr5_1:41307682-41307701 Msa0731100:CDS 30.0%
!!! GTAGTAAAAAGGTGTCTTTT+GGG + chr5_1:41307847-41307866 None:intergenic 30.0%
!!! AGTAGTAAAAAGGTGTCTTT+TGG + chr5_1:41307848-41307867 None:intergenic 30.0%
! AAAAGACACCTTTTTACTAC+TGG - chr5_1:41307847-41307866 Msa0731100:intron 30.0%
!!! AGTTTTTTCGTGTTCACAAA+CGG + chr5_1:41307880-41307899 None:intergenic 30.0%
! TGTAGTTGATTCAAATGTTG+TGG - chr5_1:41308179-41308198 Msa0731100:intron 30.0%
GAAGATTCATCAATCTTCAA+AGG + chr5_1:41308252-41308271 None:intergenic 30.0%
!!! TCTTTTTTAGAGCTTCAAAG+AGG - chr5_1:41308756-41308775 Msa0731100:intron 30.0%
! GTGGAGCAAATAAATTTTGT+TGG + chr5_1:41308786-41308805 None:intergenic 30.0%
ACAAAATTTATTTGCTCCAC+CGG - chr5_1:41308786-41308805 Msa0731100:intron 30.0%
CTCTTATTCAACCTCTTAAT+TGG - chr5_1:41308829-41308848 Msa0731100:intron 30.0%
ACTAATACCCATGTAATTCA+TGG + chr5_1:41308896-41308915 None:intergenic 30.0%
TTGTGAATTGATACTTGTAC+CGG + chr5_1:41308947-41308966 None:intergenic 30.0%
TCAAGAGAATTATGTGACTA+TGG - chr5_1:41308978-41308997 Msa0731100:intron 30.0%
! GTTGAATAATGTTTTCAGCA+AGG + chr5_1:41309124-41309143 None:intergenic 30.0%
TAGGGAAGTTATAGATAACT+TGG - chr5_1:41309306-41309325 Msa0731100:intron 30.0%
!! TTCCCTTTTATTAGTGTTGA+AGG + chr5_1:41309569-41309588 None:intergenic 30.0%
! ATAAATAATGGAGAAGCTTC+AGG - chr5_1:41309881-41309900 Msa0731100:CDS 30.0%
! TAAATAATGGAGAAGCTTCA+GGG - chr5_1:41309882-41309901 Msa0731100:CDS 30.0%
TCTCTTTATCATAGGTTAAC+TGG - chr5_1:41310099-41310118 Msa0731100:CDS 30.0%
CTCTTTATCATAGGTTAACT+GGG - chr5_1:41310100-41310119 Msa0731100:CDS 30.0%
! TACCTTGTTTAGTGATAACA+AGG - chr5_1:41310247-41310266 Msa0731100:CDS 30.0%
! ACCTTGTTTAGTGATAACAA+GGG - chr5_1:41310248-41310267 Msa0731100:CDS 30.0%
AACTCTATCATAGGAATGAA+GGG + chr5_1:41310278-41310297 None:intergenic 30.0%
TGAACCAAACATTGTTGAAT+TGG - chr5_1:41310312-41310331 Msa0731100:CDS 30.0%
GAACCAAACATTGTTGAATT+GGG - chr5_1:41310313-41310332 Msa0731100:CDS 30.0%
AACCAAACATTGTTGAATTG+GGG - chr5_1:41310314-41310333 Msa0731100:CDS 30.0%
CATGATCATTTATCTCTATC+AGG - chr5_1:41310368-41310387 Msa0731100:CDS 30.0%
CTTAGTAGTTTGAACACATT+CGG - chr5_1:41310485-41310504 Msa0731100:CDS 30.0%
TTAGTAGTTTGAACACATTC+GGG - chr5_1:41310486-41310505 Msa0731100:CDS 30.0%
AATTTCATTGGATCCTTTAG+AGG + chr5_1:41310639-41310658 None:intergenic 30.0%
TTCTTCTTCATCAACAACCT+CGG + chr5_1:41307623-41307642 None:intergenic 35.0%
! GTTGTTGATGAAGAAGAAGA+CGG - chr5_1:41307625-41307644 Msa0731100:CDS 35.0%
GAGAATTTAAGTCAAGCTTG+GGG + chr5_1:41307747-41307766 None:intergenic 35.0%
GGAGAATTTAAGTCAAGCTT+GGG + chr5_1:41307748-41307767 None:intergenic 35.0%
AGGAGAATTTAAGTCAAGCT+TGG + chr5_1:41307749-41307768 None:intergenic 35.0%
! GTTTTCATGAAAAACACTGC+CGG - chr5_1:41307919-41307938 Msa0731100:intron 35.0%
GATTCCAACAACTATATCTC+CGG + chr5_1:41307941-41307960 None:intergenic 35.0%
AGCAGTGAATAATAAGAAGG+AGG - chr5_1:41307996-41308015 Msa0731100:intron 35.0%
AGTGAATAATAAGAAGGAGG+AGG - chr5_1:41307999-41308018 Msa0731100:intron 35.0%
GTGAATAATAAGAAGGAGGA+GGG - chr5_1:41308000-41308019 Msa0731100:intron 35.0%
TATATTACCCTAGGGCTAAT+TGG - chr5_1:41308148-41308167 Msa0731100:intron 35.0%
ATGATGCTATGAAGATTGGT+TGG - chr5_1:41308202-41308221 Msa0731100:intron 35.0%
! TGATGCTATGAAGATTGGTT+GGG - chr5_1:41308203-41308222 Msa0731100:intron 35.0%
!!! ATGACATTTTTTCAGCCTCA+AGG - chr5_1:41308282-41308301 Msa0731100:intron 35.0%
ACATTAGAGATTGTTCCTTG+AGG + chr5_1:41308300-41308319 None:intergenic 35.0%
! ATTCGCATTTCGCTTTGTTA+CGG - chr5_1:41308489-41308508 Msa0731100:intron 35.0%
AAGAATTCGATGCATGCATA+CGG - chr5_1:41308533-41308552 Msa0731100:intron 35.0%
CAATACTATGAATCGATGCA+AGG + chr5_1:41308568-41308587 None:intergenic 35.0%
!! AAAAAAAGGTTTTCGGGAGT+GGG + chr5_1:41308675-41308694 None:intergenic 35.0%
!! AAAAAAAAGGTTTTCGGGAG+TGG + chr5_1:41308676-41308695 None:intergenic 35.0%
ATCAAAGAGGACCAATTAAG+AGG + chr5_1:41308843-41308862 None:intergenic 35.0%
CCAATCTCCATGAATTACAT+GGG - chr5_1:41308886-41308905 Msa0731100:intron 35.0%
!! TTCGTAAAAGTACCACAAAC+CGG - chr5_1:41308925-41308944 Msa0731100:intron 35.0%
AATCATATGAGGAGCAAGAA+GGG + chr5_1:41309810-41309829 None:intergenic 35.0%
GTCAATCACTCAATCATATG+AGG + chr5_1:41309821-41309840 None:intergenic 35.0%
! AAAGAGTGACAAGTGTTTTC+AGG - chr5_1:41309855-41309874 Msa0731100:CDS 35.0%
GAAATAAGTTCAACTTGCCA+AGG + chr5_1:41310164-41310183 None:intergenic 35.0%
!!! GGCTTTTTTGTTGAGAGAAA+TGG + chr5_1:41310209-41310228 None:intergenic 35.0%
! AAAAAAGCCTAGACTAGTTC+AGG - chr5_1:41310220-41310239 Msa0731100:CDS 35.0%
! ACCCTTGTTATCACTAAACA+AGG + chr5_1:41310252-41310271 None:intergenic 35.0%
GAACTCTATCATAGGAATGA+AGG + chr5_1:41310279-41310298 None:intergenic 35.0%
! TTTGGTTCAAAGATCTCTGA+GGG + chr5_1:41310301-41310320 None:intergenic 35.0%
CGAATGTGTTCAAACTACTA+AGG + chr5_1:41310487-41310506 None:intergenic 35.0%
ACTCACAACAACAACACAAA+AGG - chr5_1:41310524-41310543 Msa0731100:CDS 35.0%
AACAACAACACAAAAGGAGT+TGG - chr5_1:41310530-41310549 Msa0731100:CDS 35.0%
ATCATGGACATCACTTTCAA+CGG + chr5_1:41310594-41310613 None:intergenic 35.0%
! AGATTTTGAATTTCACGCGG+CGG + chr5_1:41307357-41307376 None:intergenic 40.0%
! GCCAGATTTTGAATTTCACG+CGG + chr5_1:41307360-41307379 None:intergenic 40.0%
CAAACAATCGCCGTTATTGA+TGG - chr5_1:41307481-41307500 Msa0731100:CDS 40.0%
AGGACGGTAACCATCAATAA+CGG + chr5_1:41307494-41307513 None:intergenic 40.0%
TTCCTTTCCCTGTATCATCA+CGG - chr5_1:41307513-41307532 Msa0731100:CDS 40.0%
AGTTCTTGTCCTACAGTTAC+CGG + chr5_1:41307598-41307617 None:intergenic 40.0%
TCTTCTTCATCAACAACCTC+GGG + chr5_1:41307622-41307641 None:intergenic 40.0%
! AAATTCTCCTTTTCCGTCTC+AGG - chr5_1:41307759-41307778 Msa0731100:CDS 40.0%
TCCGTTACTGATGTTCATGA+CGG - chr5_1:41307787-41307806 Msa0731100:CDS 40.0%
TCCGTCATGAACATCAGTAA+CGG + chr5_1:41307791-41307810 None:intergenic 40.0%
! AAGTTTGCTCATGTCTATCG+AGG - chr5_1:41307817-41307836 Msa0731100:CDS 40.0%
! AGTTTGCTCATGTCTATCGA+GGG - chr5_1:41307818-41307837 Msa0731100:CDS 40.0%
! GACACCTTTTTACTACTGGT+TGG - chr5_1:41307851-41307870 Msa0731100:intron 40.0%
GAGTCCAACCAGTAGTAAAA+AGG + chr5_1:41307858-41307877 None:intergenic 40.0%
AACACGAAAAAACTCGTTGC+TGG - chr5_1:41307886-41307905 Msa0731100:intron 40.0%
! CAGCAAACTTCATGTTTCTG+CGG + chr5_1:41307963-41307982 None:intergenic 40.0%
! AGAGAATGGTTTGGAGGTTA+AGG - chr5_1:41308026-41308045 Msa0731100:intron 40.0%
! GAGAATGGTTTGGAGGTTAA+GGG - chr5_1:41308027-41308046 Msa0731100:intron 40.0%
AATTGCACCAATTAGCCCTA+GGG + chr5_1:41308158-41308177 None:intergenic 40.0%
AAATTGCACCAATTAGCCCT+AGG + chr5_1:41308159-41308178 None:intergenic 40.0%
GTGGATGATGCTATGAAGAT+TGG - chr5_1:41308198-41308217 Msa0731100:intron 40.0%
!!! CTTCAGGTGCTCTTCTTTTT+TGG - chr5_1:41308345-41308364 Msa0731100:intron 40.0%
! TTCGCATTTCGCTTTGTTAC+GGG - chr5_1:41308490-41308509 Msa0731100:intron 40.0%
AGAGAAAGAAAAATCTCCGG+TGG + chr5_1:41308805-41308824 None:intergenic 40.0%
CAAGTGTGTGAAGATCAAAG+AGG + chr5_1:41308856-41308875 None:intergenic 40.0%
TGATCTTCACACACTTGTCT+CGG - chr5_1:41308859-41308878 Msa0731100:intron 40.0%
GCCAATCTCCATGAATTACA+TGG - chr5_1:41308885-41308904 Msa0731100:intron 40.0%
CCCATGTAATTCATGGAGAT+TGG + chr5_1:41308889-41308908 None:intergenic 40.0%
!! TTGATACTTGTACCGGTTTG+TGG + chr5_1:41308940-41308959 None:intergenic 40.0%
! GTTTTCAGCAAGGAATGTGT+TGG + chr5_1:41309114-41309133 None:intergenic 40.0%
!! GTTGTGATTGTGAGCATTGA+AGG + chr5_1:41309196-41309215 None:intergenic 40.0%
!! CACTTTTGCTGCTTCTATTG+CGG + chr5_1:41309262-41309281 None:intergenic 40.0%
ACAATGATCAAGGCTCTCAA+GGG + chr5_1:41309352-41309371 None:intergenic 40.0%
AACAATGATCAAGGCTCTCA+AGG + chr5_1:41309353-41309372 None:intergenic 40.0%
ACTTGGAGCAACAATGATCA+AGG + chr5_1:41309362-41309381 None:intergenic 40.0%
! GGGTTAAGTTATAGGAGTGT+AGG - chr5_1:41309430-41309449 Msa0731100:intron 40.0%
!! ATAGCTTTGTTCTGCCCTAT+CGG - chr5_1:41309460-41309479 Msa0731100:intron 40.0%
CAATCATATGAGGAGCAAGA+AGG + chr5_1:41309811-41309830 None:intergenic 40.0%
! GTAGAATCCTGAACTAGTCT+AGG + chr5_1:41310230-41310249 None:intergenic 40.0%
! GTTTGGTTCAAAGATCTCTG+AGG + chr5_1:41310302-41310321 None:intergenic 40.0%
AGCCCCAATTCAACAATGTT+TGG + chr5_1:41310319-41310338 None:intergenic 40.0%
! AACACAAAAGGAGTTGGTTC+TGG - chr5_1:41310536-41310555 Msa0731100:CDS 40.0%
GAAAGTGATGTCCATGATTC+TGG - chr5_1:41310596-41310615 Msa0731100:CDS 40.0%
TTTAGAGGAATCTTCACCCT+TGG + chr5_1:41310624-41310643 None:intergenic 40.0%
AAGGGTGAAGATTCCTCTAA+AGG - chr5_1:41310623-41310642 Msa0731100:CDS 40.0%
! GATTTTGAATTTCACGCGGC+GGG + chr5_1:41307356-41307375 None:intergenic 45.0%
GCCGCGTGAAATTCAAAATC+TGG - chr5_1:41307356-41307375 Msa0731100:CDS 45.0%
AAAATCTGGCAGACATGTGC+CGG - chr5_1:41307370-41307389 Msa0731100:CDS 45.0%
TTACTTCCCACAAGGTCACT+TGG - chr5_1:41307432-41307451 Msa0731100:CDS 45.0%
CATGTTCCAAGTGACCTTGT+GGG + chr5_1:41307441-41307460 None:intergenic 45.0%
TTGTTTGAGCGTTAGGGGAA+GGG + chr5_1:41307468-41307487 None:intergenic 45.0%
ATTGTTTGAGCGTTAGGGGA+AGG + chr5_1:41307469-41307488 None:intergenic 45.0%
TGATACAGGGAAAGGAAGGA+CGG + chr5_1:41307510-41307529 None:intergenic 45.0%
GTGATGATACAGGGAAAGGA+AGG + chr5_1:41307514-41307533 None:intergenic 45.0%
CAATTTGGCGAAGACCTCAT+CGG + chr5_1:41307566-41307585 None:intergenic 45.0%
TACCGGACTGAGTAGCAATT+TGG + chr5_1:41307581-41307600 None:intergenic 45.0%
TCTGATTCTAATAACGGCGG+TGG - chr5_1:41307685-41307704 Msa0731100:CDS 45.0%
GGAAGATCAAGTCAGCACAA+AGG + chr5_1:41307726-41307745 None:intergenic 45.0%
! CTGATGTTCATGACGGAGTT+TGG - chr5_1:41307794-41307813 Msa0731100:CDS 45.0%
ACTGCCGGAGATATAGTTGT+TGG - chr5_1:41307934-41307953 Msa0731100:intron 45.0%
AAGGAGGAGGGTAAAGAGAA+TGG - chr5_1:41308012-41308031 Msa0731100:intron 45.0%
GGAGGGTAAAGAGAATGGTT+TGG - chr5_1:41308017-41308036 Msa0731100:intron 45.0%
! GGGTAAAGAGAATGGTTTGG+AGG - chr5_1:41308020-41308039 Msa0731100:intron 45.0%
! AAGGGAGATGGTTTTTCTCG+AGG - chr5_1:41308045-41308064 Msa0731100:intron 45.0%
GGAGTTGGCGGAAAAGAATA+TGG - chr5_1:41308113-41308132 Msa0731100:intron 45.0%
! ATGAAGATTGGTTGGGCATC+TGG - chr5_1:41308210-41308229 Msa0731100:intron 45.0%
ATGTGTCCAGTGTCCCTAAT+TGG - chr5_1:41308316-41308335 Msa0731100:intron 45.0%
! TTCGCTTTGTTACGGGTTTG+CGG - chr5_1:41308497-41308516 Msa0731100:intron 45.0%
AATTCGATGCATGCATACGG+AGG - chr5_1:41308536-41308555 Msa0731100:intron 45.0%
!! ATGTGTTGGTTGAGCTGAGA+TGG + chr5_1:41309100-41309119 None:intergenic 45.0%
CGCAATAGAAGCAGCAAAAG+TGG - chr5_1:41309260-41309279 Msa0731100:intron 45.0%
! AGAAGCTTCAGGGTGTCTTA+TGG - chr5_1:41309892-41309911 Msa0731100:CDS 45.0%
ATCAAAACCGCGTCAATCCT+TGG - chr5_1:41310144-41310163 Msa0731100:CDS 45.0%
AGAAATGGTAGATGCACTGC+AGG + chr5_1:41310194-41310213 None:intergenic 45.0%
! CTCTGAGGGAACTCTATCAT+AGG + chr5_1:41310287-41310306 None:intergenic 45.0%
!! AATTGGGGCTATTTTCCAGC+AGG - chr5_1:41310329-41310348 Msa0731100:CDS 45.0%
! TATTTTCCAGCAGGCATGCA+GGG - chr5_1:41310338-41310357 Msa0731100:CDS 45.0%
TGTCCATGATTCTGGTACCA+AGG - chr5_1:41310604-41310623 Msa0731100:CDS 45.0%
GTCCATGATTCTGGTACCAA+GGG - chr5_1:41310605-41310624 Msa0731100:CDS 45.0%
!! TAATATTACAAAAAATTAAA+AGG - chr5_1:41308619-41308638 Msa0731100:intron 5.0%
!! AATTTAGTATATAAATATTA+GGG - chr5_1:41309410-41309429 Msa0731100:intron 5.0%
TCCATCGGTGAACGTTCCAA+AGG - chr5_1:41307393-41307412 Msa0731100:CDS 50.0%
!! ACCTTTGGAACGTTCACCGA+TGG + chr5_1:41307397-41307416 None:intergenic 50.0%
GCATGTTCCAAGTGACCTTG+TGG + chr5_1:41307442-41307461 None:intergenic 50.0%
GGCGATTGTTTGAGCGTTAG+GGG + chr5_1:41307473-41307492 None:intergenic 50.0%
CGGCGATTGTTTGAGCGTTA+GGG + chr5_1:41307474-41307493 None:intergenic 50.0%
ACGGCGATTGTTTGAGCGTT+AGG + chr5_1:41307475-41307494 None:intergenic 50.0%
TCAACGGCCGTGATGATACA+GGG + chr5_1:41307523-41307542 None:intergenic 50.0%
ATCAACGGCCGTGATGATAC+AGG + chr5_1:41307524-41307543 None:intergenic 50.0%
AGGATCGGCTAGGAGATCAA+CGG + chr5_1:41307539-41307558 None:intergenic 50.0%
CGCCAAATTGCTACTCAGTC+CGG - chr5_1:41307576-41307595 Msa0731100:CDS 50.0%
CTACTCAGTCCGGTAACTGT+AGG - chr5_1:41307586-41307605 Msa0731100:CDS 50.0%
TGTAGGACAAGAACTTCCCG+AGG - chr5_1:41307603-41307622 Msa0731100:CDS 50.0%
ATCAAGTCAGCACAAAGGCG+AGG + chr5_1:41307721-41307740 None:intergenic 50.0%
GAGAGTTCCTGAGACGGAAA+AGG + chr5_1:41307769-41307788 None:intergenic 50.0%
! GTAACGGAGAGTTCCTGAGA+CGG + chr5_1:41307775-41307794 None:intergenic 50.0%
GGTTTGGAGGTTAAGGGAGA+TGG - chr5_1:41308033-41308052 Msa0731100:intron 50.0%
! GGAGATGGTTTTTCTCGAGG+CGG - chr5_1:41308048-41308067 Msa0731100:intron 50.0%
! GAGATGGTTTTTCTCGAGGC+GGG - chr5_1:41308049-41308068 Msa0731100:intron 50.0%
! TTTTTCTCGAGGCGGGAGAA+GGG - chr5_1:41308056-41308075 Msa0731100:intron 50.0%
AAGGGGAATGCTAACCGAGA+AGG - chr5_1:41308074-41308093 Msa0731100:intron 50.0%
AACCGAGAAGGCGGTTATTG+AGG - chr5_1:41308086-41308105 Msa0731100:intron 50.0%
TGCCTCAATAACCGCCTTCT+CGG + chr5_1:41308091-41308110 None:intergenic 50.0%
GGTTATTGAGGCAGTGGAGT+TGG - chr5_1:41308098-41308117 Msa0731100:intron 50.0%
TATTGAGGCAGTGGAGTTGG+CGG - chr5_1:41308101-41308120 Msa0731100:intron 50.0%
CCTGAAGCATGCGCCAATTA+GGG + chr5_1:41308332-41308351 None:intergenic 50.0%
ACCTGAAGCATGCGCCAATT+AGG + chr5_1:41308333-41308352 None:intergenic 50.0%
CTTCACACACTTGTCTCGGT+TGG - chr5_1:41308863-41308882 Msa0731100:intron 50.0%
AGCTGAGATGGTCAGAGGAT+TGG + chr5_1:41309088-41309107 None:intergenic 50.0%
CAACTTGCCAAGGATTGACG+CGG + chr5_1:41310154-41310173 None:intergenic 50.0%
TAGATGCACTGCAGGATTGC+GGG + chr5_1:41310186-41310205 None:intergenic 50.0%
GTAGATGCACTGCAGGATTG+CGG + chr5_1:41310187-41310206 None:intergenic 50.0%
! CTATTTTCCAGCAGGCATGC+AGG - chr5_1:41310337-41310356 Msa0731100:CDS 50.0%
GGCGACAAGTCGTTAGATGT+GGG + chr5_1:41310458-41310477 None:intergenic 50.0%
TGGCGACAAGTCGTTAGATG+TGG + chr5_1:41310459-41310478 None:intergenic 50.0%
CACCCTTGGTACCAGAATCA+TGG + chr5_1:41310610-41310629 None:intergenic 50.0%
TGGAACGTTCACCGATGGAC+CGG + chr5_1:41307392-41307411 None:intergenic 55.0%
GGCCGTGATGATACAGGGAA+AGG + chr5_1:41307518-41307537 None:intergenic 55.0%
GACCTCATCGGTGTGAGGAT+CGG + chr5_1:41307554-41307573 None:intergenic 55.0%
! GTTTTTCTCGAGGCGGGAGA+AGG - chr5_1:41308055-41308074 Msa0731100:intron 55.0%
! TTTTCTCGAGGCGGGAGAAG+GGG - chr5_1:41308057-41308076 Msa0731100:intron 55.0%
GGGAATGCTAACCGAGAAGG+CGG - chr5_1:41308077-41308096 Msa0731100:intron 55.0%
GAAGGCGGTTATTGAGGCAG+TGG - chr5_1:41308092-41308111 Msa0731100:intron 55.0%
CATGCGCCAATTAGGGACAC+TGG + chr5_1:41308325-41308344 None:intergenic 55.0%
CCCTAATTGGCGCATGCTTC+AGG - chr5_1:41308329-41308348 Msa0731100:intron 55.0%
GGTTGAGCTGAGATGGTCAG+AGG + chr5_1:41309093-41309112 None:intergenic 55.0%
! TGAGGAGCAAGAAGGGTGCA+TGG + chr5_1:41309803-41309822 None:intergenic 55.0%
TTGAATTTCACGCGGCGGGC+CGG + chr5_1:41307352-41307371 None:intergenic 60.0%
! GCAGACATGTGCCGGTCCAT+CGG - chr5_1:41307378-41307397 Msa0731100:CDS 60.0%
TTGAGCGTTAGGGGAAGGGC+AGG + chr5_1:41307464-41307483 None:intergenic 60.0%
CATCGGTGTGAGGATCGGCT+AGG + chr5_1:41307549-41307568 None:intergenic 60.0%
AGCCGATCCTCACACCGATG+AGG - chr5_1:41307549-41307568 Msa0731100:CDS 60.0%
GCGAAGACCTCATCGGTGTG+AGG + chr5_1:41307559-41307578 None:intergenic 60.0%
GACAGACATGGTGGCCGGTG+GGG + chr5_1:41307326-41307345 None:intergenic 65.0%
GGACAGACATGGTGGCCGGT+GGG + chr5_1:41307327-41307346 None:intergenic 65.0%
GGAGGGACAGACATGGTGGC+CGG + chr5_1:41307331-41307350 None:intergenic 65.0%
GGCCACCATGTCTGTCCCTC+CGG - chr5_1:41307330-41307349 Msa0731100:CDS 65.0%
CTGACTCCCTGCATGCCTGC+TGG + chr5_1:41310347-41310366 None:intergenic 65.0%
CAGCAGGCATGCAGGGAGTC+AGG - chr5_1:41310345-41310364 Msa0731100:CDS 65.0%
GGGACAGACATGGTGGCCGG+TGG + chr5_1:41307328-41307347 None:intergenic 70.0%
GGCCGGAGGGACAGACATGG+TGG + chr5_1:41307335-41307354 None:intergenic 70.0%
ATTTCACGCGGCGGGCCGGA+GGG + chr5_1:41307348-41307367 None:intergenic 70.0%
AATTTCACGCGGCGGGCCGG+AGG + chr5_1:41307349-41307368 None:intergenic 70.0%
GCGGGCCGGAGGGACAGACA+TGG + chr5_1:41307338-41307357 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 41307316 41310661 41307316 ID=Msa0731100;Name=Msa0731100
chr5_1 mRNA 41307316 41310661 41307316 ID=Msa0731100-mRNA-1;Parent=Msa0731100;Name=Msa0731100-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|527
chr5_1 exon 41309627 41310661 41309627 ID=Msa0731100-mRNA-1:exon:12033;Parent=Msa0731100-mRNA-1
chr5_1 exon 41307316 41307864 41307316 ID=Msa0731100-mRNA-1:exon:12032;Parent=Msa0731100-mRNA-1
chr5_1 CDS 41309627 41310661 41309627 ID=Msa0731100-mRNA-1:cds;Parent=Msa0731100-mRNA-1
chr5_1 CDS 41307316 41307864 41307316 ID=Msa0731100-mRNA-1:cds;Parent=Msa0731100-mRNA-1
Gene Sequence

>Msa0731100

ATGTCTTCCCCACCGGCCACCATGTCTGTCCCTCCGGCCCGCCGCGTGAAATTCAAAATCTGGCAGACATGTGCCGGTCCATCGGTGAACGTTCCAAAGGTACGTTCTAAAGTTTATTACTTCCCACAAGGTCACTTGGAACATGCCTGCCCTTCCCCTAACGCTCAAACAATCGCCGTTATTGATGGTTACCGTCCTTCCTTTCCCTGTATCATCACGGCCGTTGATCTCCTAGCCGATCCTCACACCGATGAGGTCTTCGCCAAATTGCTACTCAGTCCGGTAACTGTAGGACAAGAACTTCCCGAGGTTGTTGATGAAGAAGAAGACGGTGATAAAGTTGTTTCCTTTGTAAAGACTCTTACAAAATCTGATTCTAATAACGGCGGTGGATTCTCTGTACCTCGCCTTTGTGCTGACTTGATCTTCCCCAAGCTTGACTTAAATTCTCCTTTTCCGTCTCAGGAACTCTCCGTTACTGATGTTCATGACGGAGTTTGGAAGTTTGCTCATGTCTATCGAGGGAGACCCAAAAGACACCTTTTTACTACTGGTTGGACTCCGTTTGTGAACACGAAAAAACTCGTTGCTGGTGATTCTATTGTTTTCATGAAAAACACTGCCGGAGATATAGTTGTTGGAATCCGCAGAAACATGAAGTTTGCTGCAGCTGAAGCAAAAGCAGTGAATAATAAGAAGGAGGAGGGTAAAGAGAATGGTTTGGAGGTTAAGGGAGATGGTTTTTCTCGAGGCGGGAGAAGGGGAATGCTAACCGAGAAGGCGGTTATTGAGGCAGTGGAGTTGGCGGAAAAGAATATGGCATTTGAAGTTATATATTACCCTAGGGCTAATTGGTGCAATTTTGTAGTTGATTCAAATGTTGTGGATGATGCTATGAAGATTGGTTGGGCATCTGGAATGAGAGTGAAGCTTCCTTTGAAGATTGATGAATCTTCAAATTCGAAGATGACATTTTTTCAGCCTCAAGGAACAATCTCTAATGTGTCCAGTGTCCCTAATTGGCGCATGCTTCAGGTTAACTGGGATGAACTTGAAATCTCGCAAAATCAAAACCGCGTCAATCCTTGGCAAGTTGAACTTATTTCCCGCAATCCTGCAGTGCATCTACCATTTCTCTCAACAAAAAAGCCTAGACTAGTTCAGGATTCTACCTTGTTTAGTGATAACAAGGGTGATCCCTTCATTCCTATGATAGAGTTCCCTCAGAGATCTTTGAACCAAACATTGTTGAATTGGGGCTATTTTCCAGCAGGCATGCAGGGAGTCAGGCATGATCATTTATCTCTATCAGGTTTTTCCAACTCTTTGAATGATAATAGTTATGGTTTCTTTGTGAACAATCTAAACATTGACAGTCCCACATCTAACGACTTGTCGCCAAATAACCTTAGTAGTTTGAACACATTCGGGACAGATTTTTTTGAGACTCACAACAACAACACAAAAGGAGTTGGTTCTGGTTCAATTAAGTTATTTGGTAAGATCATACAGACCGTTGAAAGTGATGTCCATGATTCTGGTACCAAGGGTGAAGATTCCTCTAAAGGATCCAATGAAATTTGA
Protein sequence

>Msa0731100

MSSPPATMSVPPARRVKFKIWQTCAGPSVNVPKVRSKVYYFPQGHLEHACPSPNAQTIAVIDGYRPSFPCIITAVDLLADPHTDEVFAKLLLSPVTVGQELPEVVDEEEDGDKVVSFVKTLTKSDSNNGGGFSVPRLCADLIFPKLDLNSPFPSQELSVTDVHDGVWKFAHVYRGRPKRHLFTTGWTPFVNTKKLVAGDSIVFMKNTAGDIVVGIRRNMKFAAAEAKAVNNKKEEGKENGLEVKGDGFSRGGRRGMLTEKAVIEAVELAEKNMAFEVIYYPRANWCNFVVDSNVVDDAMKIGWASGMRVKLPLKIDESSNSKMTFFQPQGTISNVSSVPNWRMLQVNWDELEISQNQNRVNPWQVELISRNPAVHLPFLSTKKPRLVQDSTLFSDNKGDPFIPMIEFPQRSLNQTLLNWGYFPAGMQGVRHDHLSLSGFSNSLNDNSYGFFVNNLNIDSPTSNDLSPNNLSSLNTFGTDFFETHNNNTKGVGSGSIKLFGKIIQTVESDVHDSGTKGEDSSKGSNEI*