AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0732520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0732520 MtrunA17_Chr5g0427361 93.725 255 14 2 1 255 1 253 2.71e-155 432
Msa0732520 MtrunA17_Chr7g0255961 35.204 196 96 3 26 220 64 229 8.96e-33 120
Msa0732520 MtrunA17_Chr5g0412371 34.914 232 110 7 2 224 1 200 8.70e-32 116
Msa0732520 MtrunA17_Chr1g0198801 35.616 219 104 4 31 245 152 337 1.18e-28 111
Msa0732520 MtrunA17_Chr2g0325521 35.417 192 98 2 30 220 52 218 4.04e-25 99.8
Msa0732520 MtrunA17_Chr3g0113721 30.542 203 100 3 31 223 33 204 5.78e-24 96.3
Msa0732520 MtrunA17_Chr4g0041051 39.634 164 73 2 28 191 60 197 1.12e-21 90.9
Msa0732520 MtrunA17_Chr4g0045901 32.836 201 91 4 30 220 68 234 3.78e-15 73.2
Msa0732520 MtrunA17_Chr7g0231461 41.892 74 41 2 24 97 292 363 4.94e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0732520 AT3G25710.1 37.864 206 84 4 33 220 128 307 9.37e-32 119
Msa0732520 AT3G56770.1 36.458 192 89 3 31 220 39 199 2.27e-30 113
Msa0732520 AT1G68810.1 45.000 160 62 2 33 191 170 304 1.50e-29 114
Msa0732520 AT2G41130.1 35.233 193 97 2 28 220 58 222 7.19e-26 102
Msa0732520 AT4G38070.1 35.465 172 78 4 29 199 83 222 6.24e-23 94.4
Msa0732520 AT2G40200.1 31.847 157 79 2 33 186 59 190 2.09e-19 85.1
Msa0732520 AT4G09820.1 41.333 75 43 1 26 100 350 423 8.89e-11 62.0

Find 85 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTGAGACATGGCCGTTTC+CGG 0.216545 5_1:-44922465 Msa0732520:CDS
CAACCTCACGAGCCCGTTTC+CGG 0.259442 5_1:+44922111 None:intergenic
AACCCGTAGAATCTTCTTAA+AGG 0.270050 5_1:+44922994 None:intergenic
CGCTAACAACGACGCCTTAT+CGG 0.275455 5_1:+44922610 None:intergenic
GGAGAAGGAGGTTGAAGCTT+TGG 0.288893 5_1:-44922204 Msa0732520:CDS
CGTTTCCGGCCCAACAAAAT+CGG 0.326862 5_1:+44922125 None:intergenic
AAGAAGCATGCAAGAGTCAT+AGG 0.355128 5_1:-44922900 Msa0732520:CDS
GAAAGCTGTTATAGAGAATA+GGG 0.375468 5_1:-44922171 Msa0732520:CDS
ACTGAGACATGGCCGTTTCC+GGG 0.384662 5_1:-44922464 Msa0732520:CDS
ACAAGCAATCCGGTCGGTTC+TGG 0.398337 5_1:-44922327 Msa0732520:CDS
TGAAAGCTGTTATAGAGAAT+AGG 0.402504 5_1:-44922172 Msa0732520:CDS
TCATCACCACCTTCACCTTT+AGG 0.406150 5_1:+44922236 None:intergenic
CCGGTCGGTTCTGGCGAAAC+CGG 0.418293 5_1:-44922318 Msa0732520:CDS
GGAGGTTGAAGCTTTGGAAA+GGG 0.425401 5_1:-44922198 Msa0732520:CDS
GGCGTCGTTGTTAGCGGAAG+TGG 0.431203 5_1:-44922603 Msa0732520:CDS
TATTGCGGTGGTAGAGAGGA+AGG 0.431797 5_1:-44922416 Msa0732520:CDS
TGAATGACCTTTGTGGTGTT+AGG 0.434067 5_1:+44922814 None:intergenic
GATTCTACGGGTTTGAGAGC+TGG 0.437215 5_1:-44922984 Msa0732520:CDS
GACAAAGAGTGTTGTGGTGG+TGG 0.449486 5_1:-44922264 Msa0732520:CDS
TCAAATCAAAGAAAAGATCA+TGG 0.457608 5_1:-44921972 Msa0732520:three_prime_UTR
CCTCCTTTAAGAAGATTCTA+CGG 0.460188 5_1:-44922997 Msa0732520:CDS
GAAGAAGGAAGCCGATGAAA+TGG 0.461603 5_1:-44922561 Msa0732520:CDS
GGATTGCTTGTGTTAGATCA+TGG 0.475145 5_1:+44922339 None:intergenic
CTCCTTTAAGAAGATTCTAC+GGG 0.475979 5_1:-44922996 Msa0732520:CDS
TGATGATGGAAGAGAGGAGA+AGG 0.477004 5_1:-44922219 Msa0732520:CDS
GTCATCATCTCCCCTCGAAC+CGG 0.479110 5_1:+44922299 None:intergenic
GTGACGATTCGCCATTTCAT+CGG 0.480584 5_1:+44922550 None:intergenic
CTTGTTGTTGAGGAAGAATG+AGG 0.491130 5_1:-44922081 Msa0732520:CDS
GAGGTTGAAGCTTTGGAAAG+GGG 0.493508 5_1:-44922197 Msa0732520:CDS
TGATGGAAGAGAGGAGAAGG+AGG 0.494593 5_1:-44922216 Msa0732520:CDS
AAGTGGTGCAGCACGTGAAA+CGG 0.496600 5_1:-44922586 Msa0732520:CDS
TCTTCATGCAGCGGAGAACC+TGG 0.503192 5_1:-44922509 Msa0732520:CDS
CGATAAGGCGTCGTTGTTAG+CGG 0.504065 5_1:-44922609 Msa0732520:CDS
GTTTCCGGCCCAACAAAATC+GGG 0.508277 5_1:+44922126 None:intergenic
AGGAGGTTGAAGCTTTGGAA+AGG 0.510777 5_1:-44922199 Msa0732520:CDS
TTCTGGCGAAACCGGTTCGA+GGG 0.511420 5_1:-44922310 Msa0732520:CDS
AAAGGTGAAGGTGGTGATGA+TGG 0.514370 5_1:-44922233 Msa0732520:CDS
GTTCTGGCGAAACCGGTTCG+AGG 0.514552 5_1:-44922311 Msa0732520:CDS
TCGTCTGATTCTCCCGGAAA+CGG 0.515198 5_1:+44922452 None:intergenic
ATGGCGAATCGTCACAATGA+TGG 0.517077 5_1:-44922542 Msa0732520:CDS
AGAAGCATGCAAGAGTCATA+GGG 0.522990 5_1:-44922899 Msa0732520:CDS
ATGATCTAACACAAGCAATC+CGG 0.524397 5_1:-44922337 Msa0732520:CDS
GGGCCGGAAACGGGCTCGTG+AGG 0.526347 5_1:-44922114 Msa0732520:CDS
TTGACTGTTCCTTGTTGTTG+AGG 0.530398 5_1:-44922091 Msa0732520:CDS
ACCTGGTTCAGTTAATTCTA+CGG 0.531153 5_1:-44922492 Msa0732520:CDS
GGGGAGATGATGACGGTTGG+AGG 0.534151 5_1:-44922290 Msa0732520:CDS
AGTGTGTTGTGAGGATCGAC+CGG 0.538414 5_1:-44922369 Msa0732520:CDS
GAGTTATTGCGGTGGTAGAG+AGG 0.542449 5_1:-44922420 Msa0732520:CDS
AGATGATGACGGTTGGAGGT+AGG 0.547372 5_1:-44922286 Msa0732520:CDS
AGAAACAAAGACTACTATGA+TGG 0.548632 5_1:-44921946 Msa0732520:three_prime_UTR
GTCGCCTCGTCTGATTCTCC+CGG 0.551846 5_1:+44922446 None:intergenic
GTGGAGTGGCCTAAAGGTGA+AGG 0.554899 5_1:-44922245 Msa0732520:CDS
GCACGTGAAACGGTTGAAGA+AGG 0.561695 5_1:-44922576 Msa0732520:CDS
CCGGTTTCGCCAGAACCGAC+CGG 0.567521 5_1:+44922318 None:intergenic
AGGTTCTCCGCTGCATGAAG+AGG 0.577024 5_1:+44922511 None:intergenic
CTACGGGTTTGAGAGCTGGA+TGG 0.582407 5_1:-44922980 Msa0732520:CDS
CGAGGGGAGATGATGACGGT+TGG 0.582498 5_1:-44922293 Msa0732520:CDS
GTGGTGGTGGAGTGGCCTAA+AGG 0.584300 5_1:-44922251 Msa0732520:CDS
AGGTGGTGATGATGGAAGAG+AGG 0.588110 5_1:-44922225 Msa0732520:CDS
CTCATTCTTCCTCAACAACA+AGG 0.601988 5_1:+44922082 None:intergenic
CCGTAGAATCTTCTTAAAGG+AGG 0.605275 5_1:+44922997 None:intergenic
TGACCTTTGTGGTGTTAGGA+AGG 0.606748 5_1:+44922818 None:intergenic
TCCGTAGAATTAACTGAACC+AGG 0.607098 5_1:+44922491 None:intergenic
AATGGAATGAATGACCTTTG+TGG 0.614221 5_1:+44922807 None:intergenic
GTTAGATCATGGTTCAGACC+CGG 0.622519 5_1:+44922350 None:intergenic
TCTGGCGAAACCGGTTCGAG+GGG 0.623016 5_1:-44922309 Msa0732520:CDS
GTGTGTTGTGAGGATCGACC+GGG 0.625955 5_1:-44922368 Msa0732520:CDS
GAGTCTTCCTCTTCATGCAG+CGG 0.627449 5_1:-44922518 Msa0732520:CDS
AGAGTCATAGGGAAGCTGAA+AGG 0.630828 5_1:-44922888 Msa0732520:CDS
CTACGGAGGTAACTGAGACA+TGG 0.643428 5_1:-44922475 Msa0732520:CDS
GGTTCGAGGGGAGATGATGA+CGG 0.643937 5_1:-44922297 Msa0732520:CDS
GAGTGGCCTAAAGGTGAAGG+TGG 0.645085 5_1:-44922242 Msa0732520:CDS
AGAGTGTTGTGGTGGTGGAG+TGG 0.646685 5_1:-44922259 Msa0732520:CDS
ACTGTCACTTTCATCCTCCG+TGG 0.656210 5_1:+44922389 None:intergenic
TAGGACAAAGAGTGTTGTGG+TGG 0.658865 5_1:-44922267 Msa0732520:CDS
GAGGCGACAGTGAGTTATTG+CGG 0.669787 5_1:-44922431 Msa0732520:CDS
AGGTAGGACAAAGAGTGTTG+TGG 0.684138 5_1:-44922270 Msa0732520:CDS
TGGTTCAGTTAATTCTACGG+AGG 0.696147 5_1:-44922489 Msa0732520:CDS
TCTCCTTCCTAACACCACAA+AGG 0.703951 5_1:-44922821 Msa0732520:intron
GTAGAGAGGAAGGAGAACCA+CGG 0.704194 5_1:-44922406 Msa0732520:CDS
GAAAGTGACAGTGTGTTGTG+AGG 0.711096 5_1:-44922378 Msa0732520:CDS
TCTAACACAAGCAATCCGGT+CGG 0.727880 5_1:-44922333 Msa0732520:CDS
GAGAGGAAGGAGAACCACGG+AGG 0.732941 5_1:-44922403 Msa0732520:CDS
GCGACAGTGAGTTATTGCGG+TGG 0.734204 5_1:-44922428 Msa0732520:CDS
GTTTCCGGGAGAATCAGACG+AGG 0.775354 5_1:-44922450 Msa0732520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTGTTTCTTATAAAAA+AGG + chr5_1:44922300-44922319 None:intergenic 15.0%
!!! TATAAAAATATCTGTTTTAG+TGG - chr5_1:44922346-44922365 Msa0732520:CDS 15.0%
!!! TCATTTGTTTCTTATAAAAA+AGG + chr5_1:44922300-44922319 None:intergenic 15.0%
!!! TATAAAAATATCTGTTTTAG+TGG - chr5_1:44922346-44922365 Msa0732520:CDS 15.0%
! AAGAATGTGAAGTTATGAAA+TGG + chr5_1:44922252-44922271 None:intergenic 25.0%
!!! AAAAATATCTGTTTTAGTGG+TGG - chr5_1:44922349-44922368 Msa0732520:CDS 25.0%
! TCAAATCAAAGAAAAGATCA+TGG - chr5_1:44923061-44923080 Msa0732520:five_prime_UTR 25.0%
! AAGAATGTGAAGTTATGAAA+TGG + chr5_1:44922252-44922271 None:intergenic 25.0%
!!! AAAAATATCTGTTTTAGTGG+TGG - chr5_1:44922349-44922368 Msa0732520:CDS 25.0%
! TCAAATCAAAGAAAAGATCA+TGG - chr5_1:44923061-44923080 Msa0732520:five_prime_UTR 25.0%
!!! TTCTTTTCTTCTTGGTTCTA+TGG + chr5_1:44921993-44922012 None:intergenic 30.0%
! TCTTCTTCTTCTTTTCTTCT+TGG + chr5_1:44922001-44922020 None:intergenic 30.0%
TGTGAAGTTATGAAATGGAA+TGG + chr5_1:44922247-44922266 None:intergenic 30.0%
TCAGCAAAAGTTAATGTATC+TGG - chr5_1:44922321-44922340 Msa0732520:CDS 30.0%
TGAAAGCTGTTATAGAGAAT+AGG - chr5_1:44922861-44922880 Msa0732520:CDS 30.0%
GAAAGCTGTTATAGAGAATA+GGG - chr5_1:44922862-44922881 Msa0732520:CDS 30.0%
! ACTTTTGCTAACACATGATT+AGG - chr5_1:44922975-44922994 Msa0732520:CDS 30.0%
AGAAACAAAGACTACTATGA+TGG - chr5_1:44923087-44923106 Msa0732520:five_prime_UTR 30.0%
!!! TTCTTTTCTTCTTGGTTCTA+TGG + chr5_1:44921993-44922012 None:intergenic 30.0%
! TCTTCTTCTTCTTTTCTTCT+TGG + chr5_1:44922001-44922020 None:intergenic 30.0%
TGTGAAGTTATGAAATGGAA+TGG + chr5_1:44922247-44922266 None:intergenic 30.0%
TCAGCAAAAGTTAATGTATC+TGG - chr5_1:44922321-44922340 Msa0732520:CDS 30.0%
TGAAAGCTGTTATAGAGAAT+AGG - chr5_1:44922861-44922880 Msa0732520:CDS 30.0%
GAAAGCTGTTATAGAGAATA+GGG - chr5_1:44922862-44922881 Msa0732520:CDS 30.0%
! ACTTTTGCTAACACATGATT+AGG - chr5_1:44922975-44922994 Msa0732520:CDS 30.0%
AGAAACAAAGACTACTATGA+TGG - chr5_1:44923087-44923106 Msa0732520:five_prime_UTR 30.0%
! CCTCCTTTAAGAAGATTCTA+CGG - chr5_1:44922036-44922055 Msa0732520:three_prime_UTR 35.0%
! CTCCTTTAAGAAGATTCTAC+GGG - chr5_1:44922037-44922056 Msa0732520:three_prime_UTR 35.0%
AACCCGTAGAATCTTCTTAA+AGG + chr5_1:44922042-44922061 None:intergenic 35.0%
AATGGAATGAATGACCTTTG+TGG + chr5_1:44922229-44922248 None:intergenic 35.0%
TCTTATAAAAAAGGACCCGA+GGG + chr5_1:44922291-44922310 None:intergenic 35.0%
TTCTTATAAAAAAGGACCCG+AGG + chr5_1:44922292-44922311 None:intergenic 35.0%
! ATTTTATGTGCAGTCCGATA+AGG - chr5_1:44922409-44922428 Msa0732520:CDS 35.0%
ACCTGGTTCAGTTAATTCTA+CGG - chr5_1:44922541-44922560 Msa0732520:CDS 35.0%
ATGATCTAACACAAGCAATC+CGG - chr5_1:44922696-44922715 Msa0732520:intron 35.0%
! TATAGAGAATAGGGCTTTTG+TGG - chr5_1:44922871-44922890 Msa0732520:CDS 35.0%
! CCTCCTTTAAGAAGATTCTA+CGG - chr5_1:44922036-44922055 Msa0732520:three_prime_UTR 35.0%
! CTCCTTTAAGAAGATTCTAC+GGG - chr5_1:44922037-44922056 Msa0732520:three_prime_UTR 35.0%
AACCCGTAGAATCTTCTTAA+AGG + chr5_1:44922042-44922061 None:intergenic 35.0%
AATGGAATGAATGACCTTTG+TGG + chr5_1:44922229-44922248 None:intergenic 35.0%
TCTTATAAAAAAGGACCCGA+GGG + chr5_1:44922291-44922310 None:intergenic 35.0%
TTCTTATAAAAAAGGACCCG+AGG + chr5_1:44922292-44922311 None:intergenic 35.0%
! ATTTTATGTGCAGTCCGATA+AGG - chr5_1:44922409-44922428 Msa0732520:CDS 35.0%
ACCTGGTTCAGTTAATTCTA+CGG - chr5_1:44922541-44922560 Msa0732520:CDS 35.0%
ATGATCTAACACAAGCAATC+CGG - chr5_1:44922696-44922715 Msa0732520:intron 35.0%
! TATAGAGAATAGGGCTTTTG+TGG - chr5_1:44922871-44922890 Msa0732520:CDS 35.0%
CCGTAGAATCTTCTTAAAGG+AGG + chr5_1:44922039-44922058 None:intergenic 40.0%
! AAGAAGCATGCAAGAGTCAT+AGG - chr5_1:44922133-44922152 Msa0732520:CDS 40.0%
! AGAAGCATGCAAGAGTCATA+GGG - chr5_1:44922134-44922153 Msa0732520:CDS 40.0%
TGAATGACCTTTGTGGTGTT+AGG + chr5_1:44922222-44922241 None:intergenic 40.0%
CTGCAAAAATACTAGTCCCT+CGG - chr5_1:44922272-44922291 Msa0732520:CDS 40.0%
TGCAAAAATACTAGTCCCTC+GGG - chr5_1:44922273-44922292 Msa0732520:CDS 40.0%
TCCGTAGAATTAACTGAACC+AGG + chr5_1:44922545-44922564 None:intergenic 40.0%
TGGTTCAGTTAATTCTACGG+AGG - chr5_1:44922544-44922563 Msa0732520:CDS 40.0%
GGATTGCTTGTGTTAGATCA+TGG + chr5_1:44922697-44922716 None:intergenic 40.0%
! AATAGGGCTTTTGTGGATTC+TGG - chr5_1:44922878-44922897 Msa0732520:CDS 40.0%
! ATAGGGCTTTTGTGGATTCT+GGG - chr5_1:44922879-44922898 Msa0732520:CDS 40.0%
!! TTGACTGTTCCTTGTTGTTG+AGG - chr5_1:44922942-44922961 Msa0732520:CDS 40.0%
CTCATTCTTCCTCAACAACA+AGG + chr5_1:44922954-44922973 None:intergenic 40.0%
!! CTTGTTGTTGAGGAAGAATG+AGG - chr5_1:44922952-44922971 Msa0732520:CDS 40.0%
CCGTAGAATCTTCTTAAAGG+AGG + chr5_1:44922039-44922058 None:intergenic 40.0%
! AAGAAGCATGCAAGAGTCAT+AGG - chr5_1:44922133-44922152 Msa0732520:CDS 40.0%
! AGAAGCATGCAAGAGTCATA+GGG - chr5_1:44922134-44922153 Msa0732520:CDS 40.0%
TGAATGACCTTTGTGGTGTT+AGG + chr5_1:44922222-44922241 None:intergenic 40.0%
CTGCAAAAATACTAGTCCCT+CGG - chr5_1:44922272-44922291 Msa0732520:CDS 40.0%
TGCAAAAATACTAGTCCCTC+GGG - chr5_1:44922273-44922292 Msa0732520:CDS 40.0%
TCCGTAGAATTAACTGAACC+AGG + chr5_1:44922545-44922564 None:intergenic 40.0%
TGGTTCAGTTAATTCTACGG+AGG - chr5_1:44922544-44922563 Msa0732520:CDS 40.0%
GGATTGCTTGTGTTAGATCA+TGG + chr5_1:44922697-44922716 None:intergenic 40.0%
! AATAGGGCTTTTGTGGATTC+TGG - chr5_1:44922878-44922897 Msa0732520:CDS 40.0%
! ATAGGGCTTTTGTGGATTCT+GGG - chr5_1:44922879-44922898 Msa0732520:CDS 40.0%
!! TTGACTGTTCCTTGTTGTTG+AGG - chr5_1:44922942-44922961 Msa0732520:CDS 40.0%
CTCATTCTTCCTCAACAACA+AGG + chr5_1:44922954-44922973 None:intergenic 40.0%
!! CTTGTTGTTGAGGAAGAATG+AGG - chr5_1:44922952-44922971 Msa0732520:CDS 40.0%
AGAGTCATAGGGAAGCTGAA+AGG - chr5_1:44922145-44922164 Msa0732520:CDS 45.0%
TCTCCTTCCTAACACCACAA+AGG - chr5_1:44922212-44922231 Msa0732520:CDS 45.0%
! TGACCTTTGTGGTGTTAGGA+AGG + chr5_1:44922218-44922237 None:intergenic 45.0%
!! TTTAGTGGTGGCTCTGAACA+CGG - chr5_1:44922361-44922380 Msa0732520:CDS 45.0%
GAAGAAGGAAGCCGATGAAA+TGG - chr5_1:44922472-44922491 Msa0732520:CDS 45.0%
GTGACGATTCGCCATTTCAT+CGG + chr5_1:44922486-44922505 None:intergenic 45.0%
ATGGCGAATCGTCACAATGA+TGG - chr5_1:44922491-44922510 Msa0732520:CDS 45.0%
GAAAGTGACAGTGTGTTGTG+AGG - chr5_1:44922655-44922674 Msa0732520:intron 45.0%
GTTAGATCATGGTTCAGACC+CGG + chr5_1:44922686-44922705 None:intergenic 45.0%
TCTAACACAAGCAATCCGGT+CGG - chr5_1:44922700-44922719 Msa0732520:intron 45.0%
!! AGGTAGGACAAAGAGTGTTG+TGG - chr5_1:44922763-44922782 Msa0732520:intron 45.0%
!! TAGGACAAAGAGTGTTGTGG+TGG - chr5_1:44922766-44922785 Msa0732520:intron 45.0%
TCATCACCACCTTCACCTTT+AGG + chr5_1:44922800-44922819 None:intergenic 45.0%
! AAAGGTGAAGGTGGTGATGA+TGG - chr5_1:44922800-44922819 Msa0732520:intron 45.0%
!! TGATGATGGAAGAGAGGAGA+AGG - chr5_1:44922814-44922833 Msa0732520:intron 45.0%
!! AGGAGGTTGAAGCTTTGGAA+AGG - chr5_1:44922834-44922853 Msa0732520:CDS 45.0%
!! GGAGGTTGAAGCTTTGGAAA+GGG - chr5_1:44922835-44922854 Msa0732520:CDS 45.0%
!! GAGGTTGAAGCTTTGGAAAG+GGG - chr5_1:44922836-44922855 Msa0732520:CDS 45.0%
! GCTTTTGTGGATTCTGGGTT+GGG - chr5_1:44922884-44922903 Msa0732520:CDS 45.0%
AGAGTCATAGGGAAGCTGAA+AGG - chr5_1:44922145-44922164 Msa0732520:CDS 45.0%
TCTCCTTCCTAACACCACAA+AGG - chr5_1:44922212-44922231 Msa0732520:CDS 45.0%
! TGACCTTTGTGGTGTTAGGA+AGG + chr5_1:44922218-44922237 None:intergenic 45.0%
!! TTTAGTGGTGGCTCTGAACA+CGG - chr5_1:44922361-44922380 Msa0732520:CDS 45.0%
GAAGAAGGAAGCCGATGAAA+TGG - chr5_1:44922472-44922491 Msa0732520:CDS 45.0%
GTGACGATTCGCCATTTCAT+CGG + chr5_1:44922486-44922505 None:intergenic 45.0%
ATGGCGAATCGTCACAATGA+TGG - chr5_1:44922491-44922510 Msa0732520:CDS 45.0%
GAAAGTGACAGTGTGTTGTG+AGG - chr5_1:44922655-44922674 Msa0732520:intron 45.0%
GTTAGATCATGGTTCAGACC+CGG + chr5_1:44922686-44922705 None:intergenic 45.0%
TCTAACACAAGCAATCCGGT+CGG - chr5_1:44922700-44922719 Msa0732520:intron 45.0%
!! AGGTAGGACAAAGAGTGTTG+TGG - chr5_1:44922763-44922782 Msa0732520:intron 45.0%
!! TAGGACAAAGAGTGTTGTGG+TGG - chr5_1:44922766-44922785 Msa0732520:intron 45.0%
TCATCACCACCTTCACCTTT+AGG + chr5_1:44922800-44922819 None:intergenic 45.0%
! AAAGGTGAAGGTGGTGATGA+TGG - chr5_1:44922800-44922819 Msa0732520:intron 45.0%
!! TGATGATGGAAGAGAGGAGA+AGG - chr5_1:44922814-44922833 Msa0732520:intron 45.0%
!! AGGAGGTTGAAGCTTTGGAA+AGG - chr5_1:44922834-44922853 Msa0732520:CDS 45.0%
!! GGAGGTTGAAGCTTTGGAAA+GGG - chr5_1:44922835-44922854 Msa0732520:CDS 45.0%
!! GAGGTTGAAGCTTTGGAAAG+GGG - chr5_1:44922836-44922855 Msa0732520:CDS 45.0%
! GCTTTTGTGGATTCTGGGTT+GGG - chr5_1:44922884-44922903 Msa0732520:CDS 45.0%
GATTCTACGGGTTTGAGAGC+TGG - chr5_1:44922049-44922068 Msa0732520:three_prime_UTR 50.0%
CGCTAACAACGACGCCTTAT+CGG + chr5_1:44922426-44922445 None:intergenic 50.0%
!! CGATAAGGCGTCGTTGTTAG+CGG - chr5_1:44922424-44922443 Msa0732520:CDS 50.0%
AAGTGGTGCAGCACGTGAAA+CGG - chr5_1:44922447-44922466 Msa0732520:CDS 50.0%
GCACGTGAAACGGTTGAAGA+AGG - chr5_1:44922457-44922476 Msa0732520:CDS 50.0%
GAGTCTTCCTCTTCATGCAG+CGG - chr5_1:44922515-44922534 Msa0732520:CDS 50.0%
CTACGGAGGTAACTGAGACA+TGG - chr5_1:44922558-44922577 Msa0732520:CDS 50.0%
AACTGAGACATGGCCGTTTC+CGG - chr5_1:44922568-44922587 Msa0732520:CDS 50.0%
TCGTCTGATTCTCCCGGAAA+CGG + chr5_1:44922584-44922603 None:intergenic 50.0%
GAGGCGACAGTGAGTTATTG+CGG - chr5_1:44922602-44922621 Msa0732520:CDS 50.0%
GAGTTATTGCGGTGGTAGAG+AGG - chr5_1:44922613-44922632 Msa0732520:CDS 50.0%
TATTGCGGTGGTAGAGAGGA+AGG - chr5_1:44922617-44922636 Msa0732520:intron 50.0%
GTAGAGAGGAAGGAGAACCA+CGG - chr5_1:44922627-44922646 Msa0732520:intron 50.0%
!! ACTGTCACTTTCATCCTCCG+TGG + chr5_1:44922647-44922666 None:intergenic 50.0%
AGTGTGTTGTGAGGATCGAC+CGG - chr5_1:44922664-44922683 Msa0732520:intron 50.0%
!! AGATGATGACGGTTGGAGGT+AGG - chr5_1:44922747-44922766 Msa0732520:intron 50.0%
!! GACAAAGAGTGTTGTGGTGG+TGG - chr5_1:44922769-44922788 Msa0732520:intron 50.0%
!! AGGTGGTGATGATGGAAGAG+AGG - chr5_1:44922808-44922827 Msa0732520:intron 50.0%
TGATGGAAGAGAGGAGAAGG+AGG - chr5_1:44922817-44922836 Msa0732520:intron 50.0%
GGAGAAGGAGGTTGAAGCTT+TGG - chr5_1:44922829-44922848 Msa0732520:CDS 50.0%
! GGCTTTTGTGGATTCTGGGT+TGG - chr5_1:44922883-44922902 Msa0732520:CDS 50.0%
GTTTCCGGCCCAACAAAATC+GGG + chr5_1:44922910-44922929 None:intergenic 50.0%
CGTTTCCGGCCCAACAAAAT+CGG + chr5_1:44922911-44922930 None:intergenic 50.0%
! CGATTTTGTTGGGCCGGAAA+CGG - chr5_1:44922909-44922928 Msa0732520:CDS 50.0%
! GATTTTGTTGGGCCGGAAAC+GGG - chr5_1:44922910-44922929 Msa0732520:CDS 50.0%
GATTCTACGGGTTTGAGAGC+TGG - chr5_1:44922049-44922068 Msa0732520:three_prime_UTR 50.0%
CGCTAACAACGACGCCTTAT+CGG + chr5_1:44922426-44922445 None:intergenic 50.0%
!! CGATAAGGCGTCGTTGTTAG+CGG - chr5_1:44922424-44922443 Msa0732520:CDS 50.0%
AAGTGGTGCAGCACGTGAAA+CGG - chr5_1:44922447-44922466 Msa0732520:CDS 50.0%
GCACGTGAAACGGTTGAAGA+AGG - chr5_1:44922457-44922476 Msa0732520:CDS 50.0%
GAGTCTTCCTCTTCATGCAG+CGG - chr5_1:44922515-44922534 Msa0732520:CDS 50.0%
CTACGGAGGTAACTGAGACA+TGG - chr5_1:44922558-44922577 Msa0732520:CDS 50.0%
AACTGAGACATGGCCGTTTC+CGG - chr5_1:44922568-44922587 Msa0732520:CDS 50.0%
TCGTCTGATTCTCCCGGAAA+CGG + chr5_1:44922584-44922603 None:intergenic 50.0%
GAGGCGACAGTGAGTTATTG+CGG - chr5_1:44922602-44922621 Msa0732520:CDS 50.0%
GAGTTATTGCGGTGGTAGAG+AGG - chr5_1:44922613-44922632 Msa0732520:CDS 50.0%
TATTGCGGTGGTAGAGAGGA+AGG - chr5_1:44922617-44922636 Msa0732520:intron 50.0%
GTAGAGAGGAAGGAGAACCA+CGG - chr5_1:44922627-44922646 Msa0732520:intron 50.0%
!! ACTGTCACTTTCATCCTCCG+TGG + chr5_1:44922647-44922666 None:intergenic 50.0%
AGTGTGTTGTGAGGATCGAC+CGG - chr5_1:44922664-44922683 Msa0732520:intron 50.0%
!! AGATGATGACGGTTGGAGGT+AGG - chr5_1:44922747-44922766 Msa0732520:intron 50.0%
!! GACAAAGAGTGTTGTGGTGG+TGG - chr5_1:44922769-44922788 Msa0732520:intron 50.0%
!! AGGTGGTGATGATGGAAGAG+AGG - chr5_1:44922808-44922827 Msa0732520:intron 50.0%
TGATGGAAGAGAGGAGAAGG+AGG - chr5_1:44922817-44922836 Msa0732520:intron 50.0%
GGAGAAGGAGGTTGAAGCTT+TGG - chr5_1:44922829-44922848 Msa0732520:CDS 50.0%
! GGCTTTTGTGGATTCTGGGT+TGG - chr5_1:44922883-44922902 Msa0732520:CDS 50.0%
GTTTCCGGCCCAACAAAATC+GGG + chr5_1:44922910-44922929 None:intergenic 50.0%
CGTTTCCGGCCCAACAAAAT+CGG + chr5_1:44922911-44922930 None:intergenic 50.0%
! CGATTTTGTTGGGCCGGAAA+CGG - chr5_1:44922909-44922928 Msa0732520:CDS 50.0%
! GATTTTGTTGGGCCGGAAAC+GGG - chr5_1:44922910-44922929 Msa0732520:CDS 50.0%
CTACGGGTTTGAGAGCTGGA+TGG - chr5_1:44922053-44922072 Msa0732520:exon 55.0%
AGGTTCTCCGCTGCATGAAG+AGG + chr5_1:44922525-44922544 None:intergenic 55.0%
TCTTCATGCAGCGGAGAACC+TGG - chr5_1:44922524-44922543 Msa0732520:CDS 55.0%
ACTGAGACATGGCCGTTTCC+GGG - chr5_1:44922569-44922588 Msa0732520:CDS 55.0%
GTTTCCGGGAGAATCAGACG+AGG - chr5_1:44922583-44922602 Msa0732520:CDS 55.0%
GCGACAGTGAGTTATTGCGG+TGG - chr5_1:44922605-44922624 Msa0732520:CDS 55.0%
GTGTGTTGTGAGGATCGACC+GGG - chr5_1:44922665-44922684 Msa0732520:intron 55.0%
! ACAAGCAATCCGGTCGGTTC+TGG - chr5_1:44922706-44922725 Msa0732520:intron 55.0%
!! TTCTGGCGAAACCGGTTCGA+GGG - chr5_1:44922723-44922742 Msa0732520:intron 55.0%
GTCATCATCTCCCCTCGAAC+CGG + chr5_1:44922737-44922756 None:intergenic 55.0%
GGTTCGAGGGGAGATGATGA+CGG - chr5_1:44922736-44922755 Msa0732520:intron 55.0%
!! AGAGTGTTGTGGTGGTGGAG+TGG - chr5_1:44922774-44922793 Msa0732520:intron 55.0%
GTGGAGTGGCCTAAAGGTGA+AGG - chr5_1:44922788-44922807 Msa0732520:intron 55.0%
GAGTGGCCTAAAGGTGAAGG+TGG - chr5_1:44922791-44922810 Msa0732520:intron 55.0%
!! TGGGTTGGGCCCGATTTTGT+TGG - chr5_1:44922898-44922917 Msa0732520:CDS 55.0%
! GGGTTGGGCCCGATTTTGTT+GGG - chr5_1:44922899-44922918 Msa0732520:CDS 55.0%
CTACGGGTTTGAGAGCTGGA+TGG - chr5_1:44922053-44922072 Msa0732520:exon 55.0%
AGGTTCTCCGCTGCATGAAG+AGG + chr5_1:44922525-44922544 None:intergenic 55.0%
TCTTCATGCAGCGGAGAACC+TGG - chr5_1:44922524-44922543 Msa0732520:CDS 55.0%
ACTGAGACATGGCCGTTTCC+GGG - chr5_1:44922569-44922588 Msa0732520:CDS 55.0%
GTTTCCGGGAGAATCAGACG+AGG - chr5_1:44922583-44922602 Msa0732520:CDS 55.0%
GCGACAGTGAGTTATTGCGG+TGG - chr5_1:44922605-44922624 Msa0732520:CDS 55.0%
GTGTGTTGTGAGGATCGACC+GGG - chr5_1:44922665-44922684 Msa0732520:intron 55.0%
! ACAAGCAATCCGGTCGGTTC+TGG - chr5_1:44922706-44922725 Msa0732520:intron 55.0%
!! TTCTGGCGAAACCGGTTCGA+GGG - chr5_1:44922723-44922742 Msa0732520:intron 55.0%
GTCATCATCTCCCCTCGAAC+CGG + chr5_1:44922737-44922756 None:intergenic 55.0%
GGTTCGAGGGGAGATGATGA+CGG - chr5_1:44922736-44922755 Msa0732520:intron 55.0%
!! AGAGTGTTGTGGTGGTGGAG+TGG - chr5_1:44922774-44922793 Msa0732520:intron 55.0%
GTGGAGTGGCCTAAAGGTGA+AGG - chr5_1:44922788-44922807 Msa0732520:intron 55.0%
GAGTGGCCTAAAGGTGAAGG+TGG - chr5_1:44922791-44922810 Msa0732520:intron 55.0%
!! TGGGTTGGGCCCGATTTTGT+TGG - chr5_1:44922898-44922917 Msa0732520:CDS 55.0%
! GGGTTGGGCCCGATTTTGTT+GGG - chr5_1:44922899-44922918 Msa0732520:CDS 55.0%
GGCGTCGTTGTTAGCGGAAG+TGG - chr5_1:44922430-44922449 Msa0732520:CDS 60.0%
GTCGCCTCGTCTGATTCTCC+CGG + chr5_1:44922590-44922609 None:intergenic 60.0%
GAGAGGAAGGAGAACCACGG+AGG - chr5_1:44922630-44922649 Msa0732520:intron 60.0%
!! GTTCTGGCGAAACCGGTTCG+AGG - chr5_1:44922722-44922741 Msa0732520:intron 60.0%
!! TCTGGCGAAACCGGTTCGAG+GGG - chr5_1:44922724-44922743 Msa0732520:intron 60.0%
!! CGAGGGGAGATGATGACGGT+TGG - chr5_1:44922740-44922759 Msa0732520:intron 60.0%
!! GGGGAGATGATGACGGTTGG+AGG - chr5_1:44922743-44922762 Msa0732520:intron 60.0%
!! GTGGTGGTGGAGTGGCCTAA+AGG - chr5_1:44922782-44922801 Msa0732520:intron 60.0%
! TGGGCCCGATTTTGTTGGGC+CGG - chr5_1:44922903-44922922 Msa0732520:CDS 60.0%
CAACCTCACGAGCCCGTTTC+CGG + chr5_1:44922925-44922944 None:intergenic 60.0%
GGCGTCGTTGTTAGCGGAAG+TGG - chr5_1:44922430-44922449 Msa0732520:CDS 60.0%
GTCGCCTCGTCTGATTCTCC+CGG + chr5_1:44922590-44922609 None:intergenic 60.0%
GAGAGGAAGGAGAACCACGG+AGG - chr5_1:44922630-44922649 Msa0732520:intron 60.0%
!! GTTCTGGCGAAACCGGTTCG+AGG - chr5_1:44922722-44922741 Msa0732520:intron 60.0%
!! TCTGGCGAAACCGGTTCGAG+GGG - chr5_1:44922724-44922743 Msa0732520:intron 60.0%
!! CGAGGGGAGATGATGACGGT+TGG - chr5_1:44922740-44922759 Msa0732520:intron 60.0%
!! GGGGAGATGATGACGGTTGG+AGG - chr5_1:44922743-44922762 Msa0732520:intron 60.0%
!! GTGGTGGTGGAGTGGCCTAA+AGG - chr5_1:44922782-44922801 Msa0732520:intron 60.0%
! TGGGCCCGATTTTGTTGGGC+CGG - chr5_1:44922903-44922922 Msa0732520:CDS 60.0%
CAACCTCACGAGCCCGTTTC+CGG + chr5_1:44922925-44922944 None:intergenic 60.0%
CCGGTTTCGCCAGAACCGAC+CGG + chr5_1:44922718-44922737 None:intergenic 65.0%
! CCGGTCGGTTCTGGCGAAAC+CGG - chr5_1:44922715-44922734 Msa0732520:intron 65.0%
CCGGTTTCGCCAGAACCGAC+CGG + chr5_1:44922718-44922737 None:intergenic 65.0%
! CCGGTCGGTTCTGGCGAAAC+CGG - chr5_1:44922715-44922734 Msa0732520:intron 65.0%
! GGGCCGGAAACGGGCTCGTG+AGG - chr5_1:44922919-44922938 Msa0732520:CDS 75.0%
! GGGCCGGAAACGGGCTCGTG+AGG - chr5_1:44922919-44922938 Msa0732520:CDS 75.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 44921934 44923121 44921934 ID=Msa0732520;Name=Msa0732520
chr5_1 mRNA 44921934 44923121 44921934 ID=Msa0732520-mRNA-1;Parent=Msa0732520;Name=Msa0732520-mRNA-1;_AED=0.00;_eAED=0.00;_QI=96|1|1|1|1|1|2|136|255
chr5_1 exon 44921934 44922633 44921934 ID=Msa0732520-mRNA-1:exon:12762;Parent=Msa0732520-mRNA-1
chr5_1 exon 44922822 44923121 44922822 ID=Msa0732520-mRNA-1:exon:12761;Parent=Msa0732520-mRNA-1
chr5_1 five_prime_UTR 44923026 44923121 44923026 ID=Msa0732520-mRNA-1:five_prime_utr;Parent=Msa0732520-mRNA-1
chr5_1 CDS 44922822 44923025 44922822 ID=Msa0732520-mRNA-1:cds;Parent=Msa0732520-mRNA-1
chr5_1 CDS 44922070 44922633 44922070 ID=Msa0732520-mRNA-1:cds;Parent=Msa0732520-mRNA-1
chr5_1 three_prime_UTR 44921934 44922069 44921934 ID=Msa0732520-mRNA-1:three_prime_utr;Parent=Msa0732520-mRNA-1
Gene Sequence

>Msa0732520

ATGCTTCCTCCTTTAAGAAGATTCTACGGGTTTGAGAGCTGGATGGATAATAAAGACTATGAGAAAATCAACTGCGACAATTCAAAATCTGAGAGAAAATCAAAAGAAGCATGCAAGAGTCATAGGGAAGCTGAAAGGAGACGCAGACAGAGAATCAACGCTCATCTCAACACTCTTCGCTCTCTCCTTCCTAACACCACAAAGTCCGATAAGGCGTCGTTGTTAGCGGAAGTGGTGCAGCACGTGAAACGGTTGAAGAAGGAAGCCGATGAAATGGCGAATCGTCACAATGATGGAGAGTCTTCCTCTTCATGCAGCGGAGAACCTGGTTCAGTTAATTCTACGGAGGTAACTGAGACATGGCCGTTTCCGGGAGAATCAGACGAGGCGACAGTGAGTTATTGCGGTGGTAGAGAGGAAGGAGAACCACGGAGGATGAAAGTGACAGTGTGTTGTGAGGATCGACCGGGTCTGAACCATGATCTAACACAAGCAATCCGGTCGGTTCTGGCGAAACCGGTTCGAGGGGAGATGATGACGGTTGGAGGTAGGACAAAGAGTGTTGTGGTGGTGGAGTGGCCTAAAGGTGAAGGTGGTGATGATGGAAGAGAGGAGAAGGAGGTTGAAGCTTTGGAAAGGGGTTTGAAAGCTGTTATAGAGAATAGGGCTTTTGTGGATTCTGGGTTGGGCCCGATTTTGTTGGGCCGGAAACGGGCTCGTGAGGTTGACTGTTCCTTGTTGTTGAGGAAGAATGAGGACTTTTGCTAA
Protein sequence

>Msa0732520

MLPPLRRFYGFESWMDNKDYEKINCDNSKSERKSKEACKSHREAERRRRQRINAHLNTLRSLLPNTTKSDKASLLAEVVQHVKRLKKEADEMANRHNDGESSSSCSGEPGSVNSTEVTETWPFPGESDEATVSYCGGREEGEPRRMKVTVCCEDRPGLNHDLTQAIRSVLAKPVRGEMMTVGGRTKSVVVVEWPKGEGGDDGREEKEVEALERGLKAVIENRAFVDSGLGPILLGRKRAREVDCSLLLRKNEDFC*