AlfalfaGEDB | Msa0734560

M. sativa cultivar zhongmu-4 / Msa0734560

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0734560	XP_039685680.1	86.771	703	89	2	58	756	97	799	0.0	1255

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0734560	sp\|Q9SZL8\|FRS5_ARATH	27.568	584	357	11	61	642	118	637	2.71e-65	235

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0734560	D3YBB9	79.253	723	134	3	58	772	129	843	0.0	1199

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TFs/TRs:

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Gene ID	Type	Classification
Msa0734560	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0734560	MtrunA17_Chr3g0088371	82.065	184	30	1	595	775	1	184	3.58e-101	310
Msa0734560	MtrunA17_Chr3g0082121	32.407	648	354	18	61	704	88	655	4.23e-97	317
Msa0734560	MtrunA17_Chr2g0324011	30.822	584	336	11	61	642	54	571	1.00e-94	307
Msa0734560	MtrunA17_Chr1g0202341	29.483	580	336	14	64	639	99	609	1.24e-74	256
Msa0734560	MtrunA17_Chr1g0203721	26.152	673	411	14	61	716	108	711	1.78e-69	245
Msa0734560	MtrunA17_Chr8g0363841	28.205	585	348	14	62	642	64	580	1.63e-68	239
Msa0734560	MtrunA17_Chr5g0448761	25.229	654	414	12	61	705	105	692	3.09e-66	234
Msa0734560	MtrunA17_Chr2g0296781	28.141	597	365	17	98	685	61	602	2.61e-64	228
Msa0734560	MtrunA17_Chr6g0477261	29.032	527	316	14	157	676	7	482	1.59e-63	224
Msa0734560	MtrunA17_Chr1g0164591	35.312	320	192	4	108	425	1	307	1.12e-62	213

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0734560	AT4G38180.1	27.568	584	357	11	61	642	118	637	2.76e-66	235
Msa0734560	AT4G15090.1	25.723	622	395	12	100	718	121	678	1.04e-58	214
Msa0734560	AT4G15090.5	25.723	622	395	12	100	718	121	678	1.04e-58	214
Msa0734560	AT4G15090.3	25.723	622	395	12	100	718	121	678	1.04e-58	214
Msa0734560	AT4G15090.2	25.723	622	395	12	100	718	121	678	1.04e-58	214
Msa0734560	AT4G15090.4	25.723	622	395	12	100	718	121	678	1.04e-58	214
Msa0734560	AT2G27110.2	25.796	597	369	16	104	696	108	634	7.07e-52	195
Msa0734560	AT2G27110.1	25.796	597	369	16	104	696	108	634	7.07e-52	195
Msa0734560	AT2G27110.3	26.916	509	315	12	190	696	36	489	1.57e-51	192
Msa0734560	AT1G76320.3	24.417	643	394	16	60	696	41	597	6.10e-48	181

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Find 123 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGGACTGTCACATGT+TGG	0.594301	5_1:+48744190	None:intergenic
AAATGCTCTGGTTTATGTAA+TGG	0.552634	5_1:+48744040	None:intergenic
AACAAAGAAGGTTTCAAGAA+GGG	0.545537	5_1:-48744238	Msa0734560:CDS
AACAATAAATATCGGCCATT+AGG	0.388497	5_1:-48743638	Msa0734560:intron
AACAGAAATTCCTTATAGCT+CGG	0.527085	5_1:-48741719	Msa0734560:CDS
AAGAAAGACCAAATCCAATT+AGG	0.397771	5_1:-48741785	Msa0734560:CDS
AATCAGATTTCGACTACAAT+TGG	0.318540	5_1:-48742865	Msa0734560:CDS
AATCGAACAAGTGAATGGTC+TGG	0.540971	5_1:+48742662	None:intergenic
AATGAAGCTCTCTCGAAACC+AGG	0.565261	5_1:-48743788	Msa0734560:CDS
AATTCTTGATTGTTATGCAT+TGG	0.467731	5_1:-48741547	Msa0734560:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAACAAAGAAGGTTTCAAGA+AGG	-	chr5_1:48741811-48741830	Msa0734560:CDS	30.0%
AACAAAGAAGGTTTCAAGAA+GGG	-	chr5_1:48741812-48741831	Msa0734560:CDS	30.0%
TTCTCATACTCAACAAAAAC+TGG	+	chr5_1:48741878-48741897	None:intergenic	30.0%
TAAAGAAAAACCAGAGGAAA+GGG	-	chr5_1:48741901-48741920	Msa0734560:CDS	30.0%
AGAACATCTTATGAAGTGAT+GGG	-	chr5_1:48742115-48742134	Msa0734560:CDS	30.0%
TTTAAGTCCGAAAATCGAAA+AGG	+	chr5_1:48742169-48742188	None:intergenic	30.0%
TGGATATCATAGTAAAAACC+TGG	+	chr5_1:48742283-48742302	None:intergenic	30.0%
CAACAAGATTATGCTCTATT+TGG	-	chr5_1:48742355-48742374	Msa0734560:CDS	30.0%
GCCGATATTTATTGTTTGTT+CGG	+	chr5_1:48742408-48742427	None:intergenic	30.0%
AACAATAAATATCGGCCATT+AGG	-	chr5_1:48742412-48742431	Msa0734560:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	48741528	48744544	48741528	ID=Msa0734560;Name=Msa0734560
chr5_1	mRNA	48741528	48744544	48741528	ID=Msa0734560-mRNA-1;Parent=Msa0734560;Name=Msa0734560-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0.5\|0.33\|0.66\|1\|1\|3\|152\|777
chr5_1	exon	48741528	48743199	48741528	ID=Msa0734560-mRNA-1:exon:13913;Parent=Msa0734560-mRNA-1
chr5_1	exon	48743639	48744297	48743639	ID=Msa0734560-mRNA-1:exon:13912;Parent=Msa0734560-mRNA-1
chr5_1	exon	48744390	48744544	48744390	ID=Msa0734560-mRNA-1:exon:13911;Parent=Msa0734560-mRNA-1
chr5_1	CDS	48744390	48744544	48744390	ID=Msa0734560-mRNA-1:cds;Parent=Msa0734560-mRNA-1
chr5_1	CDS	48743639	48744297	48743639	ID=Msa0734560-mRNA-1:cds;Parent=Msa0734560-mRNA-1
chr5_1	CDS	48741680	48743199	48741680	ID=Msa0734560-mRNA-1:cds;Parent=Msa0734560-mRNA-1
chr5_1	three_prime_UTR	48741528	48741679	48741528	ID=Msa0734560-mRNA-1:three_prime_utr;Parent=Msa0734560-mRNA-1

Gene Sequence

>Msa0734560

ATGGGCTTCTTGGATCCAAATGGGTCGAACCCTTTTCCTAAGGCCCAAGAGACATTTATTAAATGCTATTTTGCCATGCTTTCTTGCTGTGCAGACTTGTTCACTTTTGATGTACTGTTTCATTTTCATCGTATTTGCCAGAACGTTCTCAGATTGATTTTGTCTCAACAACATTATGTGCGTTATGCTTGTAACAAAGAAGGTTTCAAGAAGGGAAGTCTGCTTAATCCTAAAAACAGGCCAACATGTGACAGTCCAGTTTTTGTTGAGTATGAGAAAGCTAAAGAAAAACCAGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGTTGGATGAAGCGTTGAATGTCTATAAGATATATAGGTGGGAAGTTGATCACTGTCATCCATTACATAAACCAGAGCATTTGCGCTACTTGAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTTGCTTTAATAAATTCTAAAGCTGGAATGTCGATGAGAACATCTTATGAAGTGATGGGTCAAGGAGTTGGAGGCACGGAGAATCTTCCTTTTCGATTTTCGGACTTAAAAAACTATCTAATGACGACTCGTCAAAAGGAGATGGTGGCTGGTGAAGCAACTGTTATTCAAGAATTCTTTAGAAATGAAGCTCTCTCGAAACCAGGTTTTTACTATGATATCCAAGTTGATGCTGCAGAAGACATAGCTAGTATTTTTTGGGCAGATGGAATTATGCAACAAGATTATGCTCTATTTGGTGATGTTGTCAGTTTTGATACAACTTACCGAACAAACAATAAATATCGGCCATTAGCTGCCTTTCTTGGTTTTGACAATCATCGTAAGAGTGTGTTATTTGGTGCTGCGCTACTGTATGATGAGACATCAGCTACTTTTGATTGGTTGTTTACAACTTTTTTGAAGTGTATGTCCAATAAGAAACCTCAAACCATATACACAGACCAGGCTGGTGCGTTGTTGAAGTCAATTCCGAATGTTTTTCAAGGTGTTTTTCATGGTCTTTGCTCATGGCACATGGGAGAGAATGCAAAGAAAAATCTCGGCTCTCGTGCAAACAGTGCATTTTTCGATGAGTTAACTAATTTGGTTTCAAATGTCGAAGATGAATCAGATTTCGACTACAATTGGGATCAGATGATGAAAAACTGTTTTGATGGGGGGCCTACTTCAGACTTTAAGTGGCTTGTTCAAACTCACTCAAATCGTATGCATTGGTCTTCAGTTTGGGTGAGGTCACATTTTACAGCTGGCTTGAAAACGACTGAGTTAAGTGAGTCTTTCAATGCTTTTCTTCGTGGATTTTTGCAGCCAGACCATTCACTTGTTCGATTCTTTAGTCATTTCAACATTATGGTTCAGAGAATGAGGGATAATCATGCTGATTTGGATTTCAAGGCTGCAAACACTAGAACAAAAAATAATTATCCCAACAGTCAGCTTATGCGGTCCGTAGTAAACAAGTATACACCATCTTGCTTTTCATTTATTCACAGTCAGTATGACCTTTCCTTCAAATACTATTATGAGGAGGACACAACAAAAGGGTCAGAATTGAACAAGTTCTTCAAAGTTTTCACAATTGAGAAGGTTGATTATAATTACGATGATGTTGATAATGATAATGAGAGGGTTTCGAATGTTGATGTTTTGGATGCAGAACTTGAAGGAAACCTTTCCCCAAGGTTTGAAGATCATAATCGACTTGATGAAAGGGTTGTCACAGTCGACATTGGGAGCAAAAGTTTTACTTGTTCATGTCGTATGTTTGAGAACCGGGGTTTCTTATGTCGACATGTTTTCAAGATTTTGGAGGTCTTAGGTGGTCATGTGCAATATCATTTTTTGAAGACTATACCTGCACAATATGTTTTAAAGCGGTGGACTAGAGATGTGCGTCCGTCTGTCGATAAGCTGAAATCCACCATGAATGTTGGCACTAAAGACACCACTGAAGCGCAACGATATCAACAAATTTGTGCTGTTACAGTTCAGCTGTCTACACGTGTTTGCGCAGATGCGGAGGCATCTGAAATATTTCTCAACGGTGTTCTTGAGGCAGGGAAAAAGGCAGAGGAGTTTCTTATTTCTAAAGGTATTCATACAGATCGATCATCCGTTACGCCTTCTAAGTCTTCAAAAGCAGTTGCTGTCTGTGAACCATCTGTTGGGACTGCACCAAAGTTCAAAGAAAGACCAAATCCAATTAGGTCAAAGAAGCGACTTAAAAGTGATTATGAAAAAGCTAGAGCAAAACAGAAATTCCTTATAGCTCGGAAGAAGCAAAAGAAAGATGATGAGGTTTTAACAAATTAA

Protein sequence

>Msa0734560

MGFLDPNGSNPFPKAQETFIKCYFAMLSCCADLFTFDVLFHFHRICQNVLRLILSQQHYVRYACNKEGFKKGSLLNPKNRPTCDSPVFVEYEKAKEKPEERVGCKAGIYLKLDEALNVYKIYRWEVDHCHPLHKPEHLRYLRSFREVNEVQGQLALINSKAGMSMRTSYEVMGQGVGGTENLPFRFSDLKNYLMTTRQKEMVAGEATVIQEFFRNEALSKPGFYYDIQVDAAEDIASIFWADGIMQQDYALFGDVVSFDTTYRTNNKYRPLAAFLGFDNHRKSVLFGAALLYDETSATFDWLFTTFLKCMSNKKPQTIYTDQAGALLKSIPNVFQGVFHGLCSWHMGENAKKNLGSRANSAFFDELTNLVSNVEDESDFDYNWDQMMKNCFDGGPTSDFKWLVQTHSNRMHWSSVWVRSHFTAGLKTTELSESFNAFLRGFLQPDHSLVRFFSHFNIMVQRMRDNHADLDFKAANTRTKNNYPNSQLMRSVVNKYTPSCFSFIHSQYDLSFKYYYEEDTTKGSELNKFFKVFTIEKVDYNYDDVDNDNERVSNVDVLDAELEGNLSPRFEDHNRLDERVVTVDIGSKSFTCSCRMFENRGFLCRHVFKILEVLGGHVQYHFLKTIPAQYVLKRWTRDVRPSVDKLKSTMNVGTKDTTEAQRYQQICAVTVQLSTRVCADAEASEIFLNGVLEAGKKAEEFLISKGIHTDRSSVTPSKSSKAVAVCEPSVGTAPKFKERPNPIRSKKRLKSDYEKARAKQKFLIARKKQKKDDEVLTN*