Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0738430 | XP_003616581.1 | 98.919 | 185 | 0 | 2 | 1 | 184 | 1 | 184 | 1.14e-131 | 376 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0738430 | sp|Q8H1D2|WOX5_ARATH | 58.011 | 181 | 61 | 5 | 14 | 184 | 7 | 182 | 5.04e-60 | 187 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0738430 | G7KBY3 | 98.919 | 185 | 0 | 2 | 1 | 184 | 1 | 184 | 5.44e-132 | 376 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0738430 | TF | HB-WOX |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0738430 | MtrunA17_Chr5g0436661 | 98.919 | 185 | 0 | 2 | 1 | 184 | 1 | 184 | 1.05e-135 | 376 |
| Msa0738430 | MtrunA17_Chr7g0238431 | 69.841 | 63 | 19 | 0 | 33 | 95 | 8 | 70 | 8.19e-28 | 103 |
| Msa0738430 | MtrunA17_Chr8g0392991 | 64.789 | 71 | 25 | 0 | 25 | 95 | 88 | 158 | 1.59e-26 | 103 |
| Msa0738430 | MtrunA17_Chr4g0043891 | 61.765 | 68 | 26 | 0 | 33 | 100 | 8 | 75 | 6.60e-26 | 99.8 |
| Msa0738430 | MtrunA17_Chr1g0153171 | 65.152 | 66 | 23 | 0 | 33 | 98 | 90 | 155 | 7.09e-26 | 99.4 |
| Msa0738430 | MtrunA17_Chr5g0405351 | 59.459 | 74 | 26 | 2 | 33 | 105 | 30 | 100 | 1.17e-24 | 97.4 |
| Msa0738430 | MtrunA17_Chr4g0031341 | 57.576 | 66 | 28 | 0 | 33 | 98 | 25 | 90 | 9.96e-24 | 94.4 |
| Msa0738430 | MtrunA17_Chr8g0385441 | 69.091 | 55 | 17 | 0 | 44 | 98 | 93 | 147 | 3.15e-20 | 82.8 |
| Msa0738430 | MtrunA17_Chr3g0125111 | 67.273 | 55 | 18 | 0 | 44 | 98 | 91 | 145 | 7.58e-20 | 81.6 |
| Msa0738430 | MtrunA17_Chr7g0223471 | 45.161 | 62 | 34 | 0 | 33 | 94 | 47 | 108 | 1.51e-15 | 73.6 |
| Msa0738430 | MtrunA17_Chr2g0283141 | 45.312 | 64 | 35 | 0 | 33 | 96 | 55 | 118 | 2.03e-15 | 73.2 |
| Msa0738430 | MtrunA17_Chr1g0212511 | 43.333 | 60 | 34 | 0 | 33 | 92 | 85 | 144 | 7.73e-12 | 62.0 |
| Msa0738430 | MtrunA17_Chr7g0222981 | 31.193 | 109 | 60 | 2 | 33 | 127 | 68 | 175 | 7.33e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0738430 | AT3G11260.1 | 58.011 | 181 | 61 | 5 | 14 | 184 | 7 | 182 | 5.13e-61 | 187 |
| Msa0738430 | AT5G05770.1 | 65.487 | 113 | 31 | 3 | 4 | 110 | 2 | 112 | 9.45e-41 | 134 |
| Msa0738430 | AT3G18010.1 | 71.429 | 63 | 18 | 0 | 33 | 95 | 76 | 138 | 7.54e-27 | 104 |
| Msa0738430 | AT2G28610.1 | 65.079 | 63 | 22 | 0 | 33 | 95 | 8 | 70 | 6.19e-26 | 100 |
| Msa0738430 | AT5G59340.1 | 57.333 | 75 | 27 | 1 | 21 | 95 | 7 | 76 | 2.72e-25 | 98.6 |
| Msa0738430 | AT1G46480.2 | 63.492 | 63 | 23 | 0 | 33 | 95 | 90 | 152 | 1.19e-24 | 95.5 |
| Msa0738430 | AT1G46480.1 | 63.492 | 63 | 23 | 0 | 33 | 95 | 90 | 152 | 7.04e-24 | 94.7 |
| Msa0738430 | AT2G17950.1 | 66.667 | 63 | 20 | 1 | 33 | 94 | 38 | 100 | 9.60e-24 | 95.1 |
| Msa0738430 | AT2G01500.1 | 67.273 | 55 | 18 | 0 | 33 | 87 | 61 | 115 | 1.13e-21 | 89.4 |
| Msa0738430 | AT2G33880.1 | 45.161 | 62 | 34 | 0 | 33 | 94 | 55 | 116 | 5.77e-15 | 72.0 |
| Msa0738430 | AT2G33880.2 | 45.161 | 62 | 34 | 0 | 33 | 94 | 55 | 116 | 6.57e-15 | 72.0 |
| Msa0738430 | AT5G45980.1 | 46.774 | 62 | 33 | 0 | 33 | 94 | 55 | 116 | 1.72e-14 | 70.5 |
| Msa0738430 | AT4G35550.1 | 42.623 | 61 | 35 | 0 | 33 | 93 | 99 | 159 | 5.73e-12 | 63.2 |
| Msa0738430 | AT1G20700.2 | 35.556 | 90 | 55 | 2 | 19 | 106 | 20 | 108 | 5.82e-12 | 60.8 |
| Msa0738430 | AT1G20700.1 | 35.556 | 90 | 55 | 2 | 19 | 106 | 82 | 170 | 1.19e-11 | 61.2 |
| Msa0738430 | AT1G20700.3 | 38.667 | 75 | 45 | 1 | 19 | 93 | 82 | 155 | 1.23e-11 | 61.2 |
Find 24 sgRNAs with CRISPR-Local
Find 92 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTTAACTTGTTCTGTTGT+TGG | 0.328649 | 5_1:-56201014 | None:intergenic |
| AGAGCAAGAATGTGTTCTAT+TGG | 0.384217 | 5_1:+56201129 | Msa0738430:CDS |
| GATTCACCAAATGAGTTCAA+TGG | 0.392997 | 5_1:-56201584 | None:intergenic |
| AGAGTCTCAATCACTCTATC+AGG | 0.412077 | 5_1:-56201551 | None:intergenic |
| ATGTACAATGGGAGAACAAA+TGG | 0.428304 | 5_1:+56201670 | Msa0738430:CDS |
| AACACCCACCATTGGACTTA+CGG | 0.439714 | 5_1:+56201693 | Msa0738430:CDS |
| GGTTTCAGAATCACAAAGCT+AGG | 0.453724 | 5_1:+56201150 | Msa0738430:CDS |
| TTCTGAATTTGATCAGTGCT+TGG | 0.470346 | 5_1:-56201071 | None:intergenic |
| AGTGGAAGCAGCAGCAGCAC+TGG | 0.493876 | 5_1:+56200969 | Msa0738430:CDS |
| AATATGGAAGAGAGCATGTC+AGG | 0.497698 | 5_1:+56200906 | None:intergenic |
| TTTGCATACACATGTACAAT+GGG | 0.499345 | 5_1:+56201659 | Msa0738430:CDS |
| AGTAGTTTCCATGGTAAAAG+TGG | 0.503916 | 5_1:+56200951 | Msa0738430:CDS |
| ACAAATGGAACACCCACCAT+TGG | 0.543359 | 5_1:+56201685 | Msa0738430:CDS |
| TCATCTGGAAGTAGTTTCCA+TGG | 0.556114 | 5_1:+56200942 | Msa0738430:CDS |
| GTTTCAGAATCACAAAGCTA+GGG | 0.556495 | 5_1:+56201151 | Msa0738430:CDS |
| GTATGCATGCAAATGAATGC+AGG | 0.569996 | 5_1:+56201624 | Msa0738430:CDS |
| AGCAGCACTGGCACAAAGTG+TGG | 0.575447 | 5_1:+56200981 | Msa0738430:CDS |
| CTCAACCGTAAGTCCAATGG+TGG | 0.576156 | 5_1:-56201698 | None:intergenic |
| GTGAATCAGAATCAGAGAAG+TGG | 0.590084 | 5_1:+56201600 | Msa0738430:CDS |
| CTGGCACAAAGTGTGGTCGT+TGG | 0.591246 | 5_1:+56200988 | Msa0738430:CDS |
| AAACTCAACCGTAAGTCCAA+TGG | 0.615710 | 5_1:-56201701 | None:intergenic |
| GTTTGCATACACATGTACAA+TGG | 0.618786 | 5_1:+56201658 | Msa0738430:CDS |
| TATGCATGCAAATGAATGCA+GGG | 0.627141 | 5_1:+56201625 | Msa0738430:CDS |
| TCAACCGTAAGTCCAATGGT+GGG | 0.711399 | 5_1:-56201697 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTATTAAATTTTTTGCTTT+AGG | + | chr5_1:56201431-56201450 | Msa0738430:intron | 15.0% |
| !!! | CTTATTAAATTTTTTGCTTT+AGG | + | chr5_1:56201431-56201450 | Msa0738430:intron | 15.0% |
| !!! | AAATTATTTTTCTAACGAGA+TGG | - | chr5_1:56201386-56201405 | None:intergenic | 20.0% |
| !!! | AAATTATTTTTCTAACGAGA+TGG | - | chr5_1:56201386-56201405 | None:intergenic | 20.0% |
| !!! | TCCAATCAACTTAGTTTTTA+TGG | + | chr5_1:56201098-56201117 | Msa0738430:CDS | 25.0% |
| !!! | ACCATAAAAACTAAGTTGAT+TGG | - | chr5_1:56201102-56201121 | None:intergenic | 25.0% |
| ! | AATGAATAAATGAGTTGTGT+CGG | - | chr5_1:56201292-56201311 | None:intergenic | 25.0% |
| !!! | TCCAATCAACTTAGTTTTTA+TGG | + | chr5_1:56201098-56201117 | Msa0738430:CDS | 25.0% |
| !!! | ACCATAAAAACTAAGTTGAT+TGG | - | chr5_1:56201102-56201121 | None:intergenic | 25.0% |
| ! | AATGAATAAATGAGTTGTGT+CGG | - | chr5_1:56201292-56201311 | None:intergenic | 25.0% |
| !! | AGTTTAACTTGTTCTGTTGT+TGG | - | chr5_1:56201017-56201036 | None:intergenic | 30.0% |
| AGTATCAGATCAAAGTTTCA+AGG | - | chr5_1:56201332-56201351 | None:intergenic | 30.0% | |
| ! | TTCTTTTCCTCATATATTGC+AGG | + | chr5_1:56201506-56201525 | Msa0738430:intron | 30.0% |
| ! | TCTTTTCCTCATATATTGCA+GGG | + | chr5_1:56201507-56201526 | Msa0738430:intron | 30.0% |
| ! | CTTTTTCCATTGAACTCATT+TGG | + | chr5_1:56201578-56201597 | Msa0738430:CDS | 30.0% |
| TTTGCATACACATGTACAAT+GGG | + | chr5_1:56201659-56201678 | Msa0738430:CDS | 30.0% | |
| !! | AGTTTAACTTGTTCTGTTGT+TGG | - | chr5_1:56201017-56201036 | None:intergenic | 30.0% |
| AGTATCAGATCAAAGTTTCA+AGG | - | chr5_1:56201332-56201351 | None:intergenic | 30.0% | |
| ! | TTCTTTTCCTCATATATTGC+AGG | + | chr5_1:56201506-56201525 | Msa0738430:intron | 30.0% |
| ! | TCTTTTCCTCATATATTGCA+GGG | + | chr5_1:56201507-56201526 | Msa0738430:intron | 30.0% |
| ! | CTTTTTCCATTGAACTCATT+TGG | + | chr5_1:56201578-56201597 | Msa0738430:CDS | 30.0% |
| TTTGCATACACATGTACAAT+GGG | + | chr5_1:56201659-56201678 | Msa0738430:CDS | 30.0% | |
| AGTAGTTTCCATGGTAAAAG+TGG | + | chr5_1:56200951-56200970 | Msa0738430:CDS | 35.0% | |
| TTCTGAATTTGATCAGTGCT+TGG | - | chr5_1:56201074-56201093 | None:intergenic | 35.0% | |
| AGAGCAAGAATGTGTTCTAT+TGG | + | chr5_1:56201129-56201148 | Msa0738430:CDS | 35.0% | |
| GTTTCAGAATCACAAAGCTA+GGG | + | chr5_1:56201151-56201170 | Msa0738430:CDS | 35.0% | |
| !!! | GGTTTCTTTTGATGATGACA+AGG | + | chr5_1:56201193-56201212 | Msa0738430:CDS | 35.0% |
| TACATGTTACATCATCGTCA+GGG | - | chr5_1:56201478-56201497 | None:intergenic | 35.0% | |
| ATACATGTTACATCATCGTC+AGG | - | chr5_1:56201479-56201498 | None:intergenic | 35.0% | |
| GATTCACCAAATGAGTTCAA+TGG | - | chr5_1:56201587-56201606 | None:intergenic | 35.0% | |
| TATGCATGCAAATGAATGCA+GGG | + | chr5_1:56201625-56201644 | Msa0738430:CDS | 35.0% | |
| GTTTGCATACACATGTACAA+TGG | + | chr5_1:56201658-56201677 | Msa0738430:CDS | 35.0% | |
| ATGTACAATGGGAGAACAAA+TGG | + | chr5_1:56201670-56201689 | Msa0738430:CDS | 35.0% | |
| AGTAGTTTCCATGGTAAAAG+TGG | + | chr5_1:56200951-56200970 | Msa0738430:CDS | 35.0% | |
| TTCTGAATTTGATCAGTGCT+TGG | - | chr5_1:56201074-56201093 | None:intergenic | 35.0% | |
| AGAGCAAGAATGTGTTCTAT+TGG | + | chr5_1:56201129-56201148 | Msa0738430:CDS | 35.0% | |
| GTTTCAGAATCACAAAGCTA+GGG | + | chr5_1:56201151-56201170 | Msa0738430:CDS | 35.0% | |
| !!! | GGTTTCTTTTGATGATGACA+AGG | + | chr5_1:56201193-56201212 | Msa0738430:CDS | 35.0% |
| TACATGTTACATCATCGTCA+GGG | - | chr5_1:56201478-56201497 | None:intergenic | 35.0% | |
| ATACATGTTACATCATCGTC+AGG | - | chr5_1:56201479-56201498 | None:intergenic | 35.0% | |
| GATTCACCAAATGAGTTCAA+TGG | - | chr5_1:56201587-56201606 | None:intergenic | 35.0% | |
| TATGCATGCAAATGAATGCA+GGG | + | chr5_1:56201625-56201644 | Msa0738430:CDS | 35.0% | |
| GTTTGCATACACATGTACAA+TGG | + | chr5_1:56201658-56201677 | Msa0738430:CDS | 35.0% | |
| ATGTACAATGGGAGAACAAA+TGG | + | chr5_1:56201670-56201689 | Msa0738430:CDS | 35.0% | |
| ! | GGCTTTTGCATGAAATCATC+TGG | + | chr5_1:56200927-56200946 | Msa0738430:CDS | 40.0% |
| TCATCTGGAAGTAGTTTCCA+TGG | + | chr5_1:56200942-56200961 | Msa0738430:CDS | 40.0% | |
| ! | TGACTGAACTTTTCAGAGCA+GGG | + | chr5_1:56201039-56201058 | Msa0738430:CDS | 40.0% |
| GGTTTCAGAATCACAAAGCT+AGG | + | chr5_1:56201150-56201169 | Msa0738430:CDS | 40.0% | |
| GGAAAGACAAAAACGTCGTA+AGG | + | chr5_1:56201172-56201191 | Msa0738430:CDS | 40.0% | |
| CTTTAGGCCTCTTAATGCAT+TGG | + | chr5_1:56201447-56201466 | Msa0738430:intron | 40.0% | |
| AGCAAACCCTGCAATATATG+AGG | - | chr5_1:56201516-56201535 | None:intergenic | 40.0% | |
| AGAGTCTCAATCACTCTATC+AGG | - | chr5_1:56201554-56201573 | None:intergenic | 40.0% | |
| GTGAATCAGAATCAGAGAAG+TGG | + | chr5_1:56201600-56201619 | Msa0738430:CDS | 40.0% | |
| GTATGCATGCAAATGAATGC+AGG | + | chr5_1:56201624-56201643 | Msa0738430:CDS | 40.0% | |
| AAACTCAACCGTAAGTCCAA+TGG | - | chr5_1:56201704-56201723 | None:intergenic | 40.0% | |
| ! | GGCTTTTGCATGAAATCATC+TGG | + | chr5_1:56200927-56200946 | Msa0738430:CDS | 40.0% |
| TCATCTGGAAGTAGTTTCCA+TGG | + | chr5_1:56200942-56200961 | Msa0738430:CDS | 40.0% | |
| ! | TGACTGAACTTTTCAGAGCA+GGG | + | chr5_1:56201039-56201058 | Msa0738430:CDS | 40.0% |
| GGTTTCAGAATCACAAAGCT+AGG | + | chr5_1:56201150-56201169 | Msa0738430:CDS | 40.0% | |
| GGAAAGACAAAAACGTCGTA+AGG | + | chr5_1:56201172-56201191 | Msa0738430:CDS | 40.0% | |
| CTTTAGGCCTCTTAATGCAT+TGG | + | chr5_1:56201447-56201466 | Msa0738430:intron | 40.0% | |
| AGCAAACCCTGCAATATATG+AGG | - | chr5_1:56201516-56201535 | None:intergenic | 40.0% | |
| AGAGTCTCAATCACTCTATC+AGG | - | chr5_1:56201554-56201573 | None:intergenic | 40.0% | |
| GTGAATCAGAATCAGAGAAG+TGG | + | chr5_1:56201600-56201619 | Msa0738430:CDS | 40.0% | |
| GTATGCATGCAAATGAATGC+AGG | + | chr5_1:56201624-56201643 | Msa0738430:CDS | 40.0% | |
| AAACTCAACCGTAAGTCCAA+TGG | - | chr5_1:56201704-56201723 | None:intergenic | 40.0% | |
| !! | TGCTGCTTCCACTTTTACCA+TGG | - | chr5_1:56200962-56200981 | None:intergenic | 45.0% |
| ! | CTGACTGAACTTTTCAGAGC+AGG | + | chr5_1:56201038-56201057 | Msa0738430:CDS | 45.0% |
| ACAAATGGAACACCCACCAT+TGG | + | chr5_1:56201685-56201704 | Msa0738430:CDS | 45.0% | |
| AACACCCACCATTGGACTTA+CGG | + | chr5_1:56201693-56201712 | Msa0738430:CDS | 45.0% | |
| TCAACCGTAAGTCCAATGGT+GGG | - | chr5_1:56201700-56201719 | None:intergenic | 45.0% | |
| !! | TGCTGCTTCCACTTTTACCA+TGG | - | chr5_1:56200962-56200981 | None:intergenic | 45.0% |
| ! | CTGACTGAACTTTTCAGAGC+AGG | + | chr5_1:56201038-56201057 | Msa0738430:CDS | 45.0% |
| ACAAATGGAACACCCACCAT+TGG | + | chr5_1:56201685-56201704 | Msa0738430:CDS | 45.0% | |
| AACACCCACCATTGGACTTA+CGG | + | chr5_1:56201693-56201712 | Msa0738430:CDS | 45.0% | |
| TCAACCGTAAGTCCAATGGT+GGG | - | chr5_1:56201700-56201719 | None:intergenic | 45.0% | |
| !!! | GTTAAAAAAATTATTTTAAA+AGG | + | chr5_1:56201407-56201426 | Msa0738430:intron | 5.0% |
| !!! | GTTAAAAAAATTATTTTAAA+AGG | + | chr5_1:56201407-56201426 | Msa0738430:intron | 5.0% |
| GGCCTCTTAATGCATTGGAC+CGG | + | chr5_1:56201452-56201471 | Msa0738430:intron | 50.0% | |
| TGTTACATCATCGTCAGGGC+CGG | - | chr5_1:56201474-56201493 | None:intergenic | 50.0% | |
| CTCAACCGTAAGTCCAATGG+TGG | - | chr5_1:56201701-56201720 | None:intergenic | 50.0% | |
| GGCCTCTTAATGCATTGGAC+CGG | + | chr5_1:56201452-56201471 | Msa0738430:intron | 50.0% | |
| TGTTACATCATCGTCAGGGC+CGG | - | chr5_1:56201474-56201493 | None:intergenic | 50.0% | |
| CTCAACCGTAAGTCCAATGG+TGG | - | chr5_1:56201701-56201720 | None:intergenic | 50.0% | |
| !! | AGCAGCACTGGCACAAAGTG+TGG | + | chr5_1:56200981-56201000 | Msa0738430:CDS | 55.0% |
| CTGGCACAAAGTGTGGTCGT+TGG | + | chr5_1:56200988-56201007 | Msa0738430:CDS | 55.0% | |
| GGCCGGTCCAATGCATTAAG+AGG | - | chr5_1:56201457-56201476 | None:intergenic | 55.0% | |
| !! | AGCAGCACTGGCACAAAGTG+TGG | + | chr5_1:56200981-56201000 | Msa0738430:CDS | 55.0% |
| CTGGCACAAAGTGTGGTCGT+TGG | + | chr5_1:56200988-56201007 | Msa0738430:CDS | 55.0% | |
| GGCCGGTCCAATGCATTAAG+AGG | - | chr5_1:56201457-56201476 | None:intergenic | 55.0% | |
| AGTGGAAGCAGCAGCAGCAC+TGG | + | chr5_1:56200969-56200988 | Msa0738430:CDS | 60.0% | |
| AGTGGAAGCAGCAGCAGCAC+TGG | + | chr5_1:56200969-56200988 | Msa0738430:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 56200909 | 56201730 | 56200909 | ID=Msa0738430;Name=Msa0738430 |
| chr5_1 | mRNA | 56200909 | 56201730 | 56200909 | ID=Msa0738430-mRNA-1;Parent=Msa0738430;Name=Msa0738430-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|1|1|2|0|184 |
| chr5_1 | exon | 56200909 | 56201260 | 56200909 | ID=Msa0738430-mRNA-1:exon:15917;Parent=Msa0738430-mRNA-1 |
| chr5_1 | exon | 56201528 | 56201730 | 56201528 | ID=Msa0738430-mRNA-1:exon:15918;Parent=Msa0738430-mRNA-1 |
| chr5_1 | CDS | 56200909 | 56201260 | 56200909 | ID=Msa0738430-mRNA-1:cds;Parent=Msa0738430-mRNA-1 |
| chr5_1 | CDS | 56201528 | 56201730 | 56201528 | ID=Msa0738430-mRNA-1:cds;Parent=Msa0738430-mRNA-1 |
| Gene Sequence |
| Protein sequence |
