AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0742250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0742250 MtrunA17_Chr5g0443531 59.286 140 23 1 1 106 847 986 1.97e-47 161
Msa0742250 MtrunA17_Chr2g0315151 65.823 79 27 0 1 79 835 913 6.52e-30 111
Msa0742250 MtrunA17_Chr8g0359691 57.500 80 34 0 1 80 833 912 8.77e-26 100
Msa0742250 MtrunA17_Chr2g0314961 46.939 98 52 0 1 98 305 402 9.81e-25 96.3
Msa0742250 MtrunA17_Chr2g0314911 52.500 80 38 0 1 80 831 910 3.29e-24 95.5
Msa0742250 MtrunA17_Chr2g0315081 52.500 80 38 0 1 80 823 902 1.83e-23 93.6
Msa0742250 MtrunA17_Chr2g0314991 52.500 80 38 0 1 80 1346 1425 2.14e-23 93.2
Msa0742250 MtrunA17_Chr2g0314991 50.000 80 40 0 1 80 380 459 1.34e-22 90.9
Msa0742250 MtrunA17_Chr8g0359641 45.918 98 53 0 1 98 836 933 4.89e-23 92.0
Msa0742250 MtrunA17_Chr5g0438071 48.750 80 41 0 1 80 829 908 6.61e-23 91.7
Msa0742250 MtrunA17_Chr8g0359651 44.898 98 54 0 1 98 836 933 8.19e-23 91.7
Msa0742250 MtrunA17_Chr2g0314901 48.750 80 41 0 1 80 838 917 4.15e-22 89.4
Msa0742250 MtrunA17_Chr2g0315011 47.500 80 42 0 1 80 9 88 6.58e-22 85.1
Msa0742250 MtrunA17_Chr2g0315061 47.500 80 42 0 1 80 206 285 4.72e-21 85.9
Msa0742250 MtrunA17_Chr8g0358781 53.333 75 34 1 1 75 511 584 5.63e-21 86.3
Msa0742250 MtrunA17_Chr8g0358821 53.165 79 36 1 1 79 856 933 6.31e-21 86.3
Msa0742250 MtrunA17_Chr2g0314881 48.750 80 41 0 1 80 398 477 1.29e-20 85.1
Msa0742250 MtrunA17_Chr2g0314841 45.000 80 44 0 1 80 830 909 8.12e-20 83.2
Msa0742250 MtrunA17_Chr2g0314821 45.000 80 44 0 1 80 833 912 9.13e-20 82.8
Msa0742250 MtrunA17_Chr8g0359581 50.633 79 30 2 1 79 83 152 4.06e-19 77.8
Msa0742250 MtrunA17_Chr2g0314861 45.000 80 44 0 1 80 89 168 5.95e-19 79.0
Msa0742250 MtrunA17_Chr1g0160921 44.872 78 43 0 1 78 220 297 2.76e-17 75.5
Msa0742250 MtrunA17_Chr8g0376131 36.975 119 62 3 1 107 221 338 3.71e-16 72.4
Msa0742250 MtrunA17_Chr2g0312561 45.570 79 43 0 1 79 212 290 8.14e-16 71.2
Msa0742250 MtrunA17_Chr8g0358911 46.835 79 42 0 1 79 215 293 2.70e-15 70.1
Msa0742250 MtrunA17_Chr2g0281971 46.053 76 40 1 1 76 218 292 9.73e-15 68.2
Msa0742250 MtrunA17_Chr3g0109461 45.000 80 42 2 2 79 509 588 2.83e-14 67.0
Msa0742250 MtrunA17_Chr5g0394001 40.789 76 45 0 1 76 500 575 8.02e-14 65.9
Msa0742250 MtrunA17_Chr4g0071371 42.466 73 42 0 2 74 305 377 1.12e-13 65.5
Msa0742250 MtrunA17_Chr3g0109411 48.000 75 38 1 2 75 494 568 1.14e-13 65.5
Msa0742250 MtrunA17_Chr2g0312571 45.570 79 43 0 1 79 217 295 1.14e-13 65.5
Msa0742250 MtrunA17_Chr3g0109431 41.772 79 45 1 2 79 497 575 2.67e-13 64.3
Msa0742250 MtrunA17_Chr7g0242201 35.849 106 66 2 1 106 213 316 3.02e-13 63.9
Msa0742250 MtrunA17_Chr2g0299041 47.692 65 34 0 2 66 283 347 3.94e-13 63.9
Msa0742250 MtrunA17_Chr4g0047561 41.667 96 55 1 1 96 216 310 5.32e-13 63.5
Msa0742250 MtrunA17_Chr8g0380441 40.260 77 37 3 2 73 248 320 5.50e-13 63.5
Msa0742250 MtrunA17_Chr7g0216551 42.466 73 41 1 2 73 302 374 6.45e-13 63.2
Msa0742250 MtrunA17_Chr5g0416011 42.466 73 41 1 2 73 314 386 7.35e-13 63.2
Msa0742250 MtrunA17_Chr8g0357831 42.105 76 44 0 1 76 362 437 7.95e-13 63.2
Msa0742250 MtrunA17_Chr4g0042441 40.789 76 45 0 1 76 670 745 1.13e-12 62.8
Msa0742250 MtrunA17_Chr2g0280761 32.895 76 51 0 1 76 1 76 1.37e-12 60.1
Msa0742250 MtrunA17_Chr7g0245571 40.845 71 41 1 2 71 266 336 2.14e-12 61.6
Msa0742250 MtrunA17_Chr2g0330471 41.333 75 44 0 1 75 575 649 2.40e-12 61.6
Msa0742250 MtrunA17_Chr6g0453561 38.158 76 47 0 1 76 37 112 2.54e-12 60.1
Msa0742250 MtrunA17_Chr8g0357651 40.789 76 45 0 1 76 640 715 2.62e-12 61.6
Msa0742250 MtrunA17_Chr4g0064201 46.053 76 38 2 2 75 288 362 2.85e-12 61.2
Msa0742250 MtrunA17_Chr4g0042351 37.333 75 47 0 1 75 623 697 2.91e-12 61.6
Msa0742250 MtrunA17_Chr8g0357631 42.105 76 44 0 1 76 638 713 2.92e-12 61.6
Msa0742250 MtrunA17_Chr2g0300711 42.667 75 34 3 2 71 254 324 2.93e-12 61.2
Msa0742250 MtrunA17_Chr8g0357661 42.105 76 44 0 1 76 33 108 3.19e-12 59.7
Msa0742250 MtrunA17_Chr1g0154311 36.986 73 46 0 1 73 521 593 3.49e-12 61.2
Msa0742250 MtrunA17_Chr8g0340091 43.243 74 39 1 1 71 702 775 3.52e-12 61.2
Msa0742250 MtrunA17_Chr6g0474991 38.961 77 46 1 1 76 33 109 3.89e-12 59.7
Msa0742250 MtrunA17_Chr1g0206231 43.750 80 37 3 1 76 516 591 4.08e-12 60.8
Msa0742250 MtrunA17_Chr8g0362661 39.726 73 43 1 2 73 312 384 4.31e-12 60.8
Msa0742250 MtrunA17_Chr6g0475091 38.961 77 46 1 1 76 751 827 5.32e-12 60.8
Msa0742250 MtrunA17_Chr7g0236231 40.909 66 39 0 1 66 688 753 6.13e-12 60.5
Msa0742250 MtrunA17_Chr1g0206331 37.333 75 47 0 1 75 505 579 8.62e-12 60.1
Msa0742250 MtrunA17_Chr5g0446651 40.506 79 46 1 1 79 528 605 9.16e-12 60.1
Msa0742250 MtrunA17_Chr4g0023111 36.842 76 48 0 1 76 884 959 9.33e-12 60.1
Msa0742250 MtrunA17_Chr4g0069061 42.105 76 41 2 2 75 86 160 1.03e-11 58.9
Msa0742250 MtrunA17_Chr8g0341881 39.241 79 48 0 1 79 573 651 1.26e-11 59.7
Msa0742250 MtrunA17_Chr8g0340051 43.243 74 39 1 1 71 199 272 1.36e-11 59.3
Msa0742250 MtrunA17_Chr8g0357761 40.789 76 45 0 1 76 650 725 1.40e-11 59.7
Msa0742250 MtrunA17_Chr8g0389251 37.975 79 49 0 1 79 572 650 1.72e-11 59.3
Msa0742250 MtrunA17_Chr2g0320111 37.975 79 48 1 1 78 267 345 1.82e-11 58.9
Msa0742250 MtrunA17_Chr8g0388941 39.726 73 43 1 2 73 246 318 1.92e-11 58.9
Msa0742250 MtrunA17_Chr1g0206361 38.158 76 47 0 1 76 513 588 2.01e-11 58.9
Msa0742250 MtrunA17_Chr6g0452761 38.356 73 44 1 2 73 311 383 2.04e-11 58.9
Msa0742250 MtrunA17_Chr1g0196381 32.990 97 61 1 1 97 285 377 2.15e-11 58.9
Msa0742250 MtrunA17_Chr1g0181821 39.759 83 43 3 2 80 246 325 2.17e-11 58.9
Msa0742250 MtrunA17_Chr8g0373501 36.634 101 58 2 1 96 504 603 2.19e-11 58.9
Msa0742250 MtrunA17_Chr1g0206351 40.789 76 45 0 1 76 523 598 2.44e-11 58.9
Msa0742250 MtrunA17_Chr8g0340081 40.909 66 39 0 1 66 677 742 3.26e-11 58.5
Msa0742250 MtrunA17_Chr8g0357711 40.789 76 45 0 1 76 647 722 3.48e-11 58.5
Msa0742250 MtrunA17_Chr7g0226891 45.455 66 34 1 1 66 218 281 3.70e-11 58.2
Msa0742250 MtrunA17_Chr3g0078761 40.506 79 46 1 1 79 515 592 3.71e-11 58.2
Msa0742250 MtrunA17_Chr6g0474811 38.961 77 46 1 1 76 743 819 4.22e-11 58.2
Msa0742250 MtrunA17_Chr6g0475131 40.260 77 45 1 1 76 179 255 5.41e-11 57.8
Msa0742250 MtrunA17_Chr4g0048581 43.939 66 36 1 1 66 701 765 6.32e-11 57.8
Msa0742250 MtrunA17_Chr2g0328171 41.333 75 41 2 2 74 254 327 7.24e-11 57.4
Msa0742250 MtrunA17_Chr4g0042381 34.667 75 49 0 1 75 623 697 7.41e-11 57.4
Msa0742250 MtrunA17_Chr2g0328201 43.421 76 40 2 1 74 49 123 7.42e-11 55.8
Msa0742250 MtrunA17_Chr4g0042391 34.667 75 49 0 1 75 623 697 8.17e-11 57.4
Msa0742250 MtrunA17_Chr3g0111631 39.744 78 46 1 2 78 256 333 8.63e-11 57.0
Msa0742250 MtrunA17_Chr5g0393981 36.842 76 48 0 1 76 566 641 9.78e-11 57.0
Msa0742250 MtrunA17_Chr2g0280681 39.474 76 46 0 1 76 671 746 9.99e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0742250 AT1G07650.4 44.526 137 40 2 1 103 635 769 7.67e-31 114
Msa0742250 AT1G07650.3 44.526 137 40 2 1 103 822 956 7.78e-31 114
Msa0742250 AT1G07650.1 44.526 137 40 2 1 103 848 982 7.79e-31 114
Msa0742250 AT1G07650.2 44.526 137 40 2 1 103 854 988 7.79e-31 114
Msa0742250 AT1G53430.2 61.250 80 31 0 1 80 797 876 7.76e-29 108
Msa0742250 AT1G53430.1 61.250 80 31 0 1 80 838 917 7.78e-29 108
Msa0742250 AT1G53440.1 61.250 80 31 0 1 80 836 915 8.09e-29 108
Msa0742250 AT1G53420.1 56.962 79 34 0 1 79 793 871 7.72e-26 100
Msa0742250 AT3G14840.2 54.430 79 36 0 1 79 841 919 2.13e-25 99.4
Msa0742250 AT1G56120.3 52.000 75 35 1 1 75 711 784 1.18e-18 80.1
Msa0742250 AT1G56120.1 52.000 75 35 1 1 75 877 950 1.21e-18 80.1
Msa0742250 AT1G56120.2 52.000 75 35 1 1 75 859 932 1.34e-18 80.1
Msa0742250 AT1G29740.2 53.731 67 30 1 1 66 844 910 2.57e-17 76.3
Msa0742250 AT1G56130.1 48.649 74 37 1 1 74 713 785 2.67e-17 76.3
Msa0742250 AT1G56130.2 48.649 74 37 1 1 74 861 933 2.70e-17 76.3
Msa0742250 AT1G56130.3 48.649 74 37 1 1 74 662 734 2.93e-17 76.3
Msa0742250 AT1G29740.1 53.731 67 30 1 1 66 844 910 3.35e-17 75.9
Msa0742250 AT1G56145.3 45.333 75 40 1 1 75 751 824 3.38e-17 75.9
Msa0742250 AT1G56145.1 45.333 75 40 1 1 75 854 927 3.40e-17 75.9
Msa0742250 AT1G56145.2 45.333 75 40 1 1 75 881 954 3.41e-17 75.9
Msa0742250 AT1G29730.2 44.565 92 50 1 1 91 840 931 4.13e-17 75.9
Msa0742250 AT1G29730.1 44.565 92 50 1 1 91 808 899 4.16e-17 75.5
Msa0742250 AT3G09010.1 35.052 97 62 1 1 97 215 310 9.62e-17 74.3
Msa0742250 AT1G56140.1 48.649 74 37 1 1 74 860 932 1.06e-16 74.3
Msa0742250 AT1G16670.1 48.649 74 38 0 1 74 212 285 1.47e-16 73.9
Msa0742250 AT1G29720.1 44.304 79 44 0 1 79 840 918 2.20e-16 73.6
Msa0742250 AT5G56460.1 44.304 79 35 3 2 75 254 328 6.04e-14 66.6
Msa0742250 AT4G05200.2 44.737 76 42 0 1 76 534 609 1.62e-13 65.5
Msa0742250 AT4G23260.2 42.857 77 43 1 1 76 497 573 1.65e-13 65.5
Msa0742250 AT4G23260.1 42.857 77 43 1 1 76 508 584 1.65e-13 65.5
Msa0742250 AT4G05200.1 44.737 76 42 0 1 76 516 591 1.65e-13 65.5
Msa0742250 AT4G23230.1 44.737 76 42 0 1 76 506 581 1.66e-13 65.5
Msa0742250 AT3G05140.1 43.750 80 41 2 1 80 311 386 2.36e-13 64.7
Msa0742250 AT3G05140.2 43.750 80 41 2 1 80 359 434 2.56e-13 64.7
Msa0742250 AT2G07180.1 34.906 106 59 4 2 102 265 365 3.04e-13 64.7
Msa0742250 AT2G07180.2 34.906 106 59 4 2 102 265 365 3.04e-13 64.7
Msa0742250 AT3G58690.1 39.241 79 47 1 1 78 260 338 6.37e-13 63.5
Msa0742250 AT2G39660.1 44.706 85 36 4 2 80 247 326 6.79e-13 63.5
Msa0742250 AT1G70740.1 38.667 75 46 0 1 75 230 304 9.32e-13 63.2
Msa0742250 AT4G23180.1 42.105 76 44 0 1 76 517 592 1.06e-12 63.2
Msa0742250 AT4G23190.1 44.872 78 40 1 1 75 519 596 1.17e-12 62.8
Msa0742250 AT4G23190.3 44.872 78 40 1 1 75 441 518 1.54e-12 62.8
Msa0742250 AT4G23190.4 44.872 78 40 1 1 75 441 518 1.54e-12 62.8
Msa0742250 AT4G23140.1 43.421 76 43 0 1 76 520 595 1.79e-12 62.4
Msa0742250 AT4G23140.2 43.421 76 43 0 1 76 526 601 1.86e-12 62.4
Msa0742250 AT5G56790.1 37.333 75 47 0 1 75 558 632 1.90e-12 62.4
Msa0742250 AT3G13690.2 39.241 79 48 0 1 79 579 657 2.13e-12 62.4
Msa0742250 AT3G13690.1 39.241 79 48 0 1 79 579 657 2.13e-12 62.4
Msa0742250 AT3G13690.3 39.241 79 48 0 1 79 579 657 2.13e-12 62.4
Msa0742250 AT1G55200.3 39.241 79 48 0 1 79 547 625 2.85e-12 62.0
Msa0742250 AT1G55200.4 39.241 79 48 0 1 79 547 625 2.85e-12 62.0
Msa0742250 AT1G55200.1 39.241 79 48 0 1 79 547 625 2.85e-12 62.0
Msa0742250 AT1G55200.2 39.241 79 48 0 1 79 547 625 2.85e-12 62.0
Msa0742250 AT4G23160.1 40.789 76 45 0 1 76 528 603 3.71e-12 61.6
Msa0742250 AT1G61860.1 44.286 70 38 1 2 70 257 326 4.11e-12 61.2
Msa0742250 AT4G23250.1 40.260 77 45 1 1 76 528 604 6.41e-12 60.8
Msa0742250 AT4G23250.3 40.260 77 45 1 1 76 523 599 6.43e-12 60.8
Msa0742250 AT5G01020.1 40.845 71 41 1 2 71 244 314 7.96e-12 60.5
Msa0742250 AT4G23250.2 40.260 77 45 1 1 76 523 599 9.99e-12 60.5
Msa0742250 AT4G11460.1 42.105 76 43 1 1 75 518 593 1.41e-11 59.7
Msa0742250 AT4G23320.2 41.558 77 44 1 1 76 352 428 1.42e-11 59.7
Msa0742250 AT4G23320.1 41.558 77 44 1 1 76 352 428 1.42e-11 59.7
Msa0742250 AT4G23320.3 41.558 77 44 1 1 76 373 449 1.43e-11 59.7
Msa0742250 AT2G26290.1 42.105 76 37 3 2 73 263 335 1.45e-11 59.7
Msa0742250 AT4G11460.3 42.105 76 43 1 1 75 515 590 1.47e-11 59.7
Msa0742250 AT4G11460.2 42.105 76 43 1 1 75 440 515 1.65e-11 59.7
Msa0742250 AT1G07570.4 44.304 79 33 4 2 74 132 205 1.82e-11 59.3
Msa0742250 AT4G23280.3 42.105 76 43 1 1 75 463 538 1.96e-11 59.3
Msa0742250 AT1G07570.1 44.304 79 33 4 2 74 248 321 1.99e-11 59.3
Msa0742250 AT1G07570.5 44.304 79 33 4 2 74 248 321 1.99e-11 59.3
Msa0742250 AT1G07570.2 44.304 79 33 4 2 74 248 321 1.99e-11 59.3
Msa0742250 AT1G07570.3 44.304 79 33 4 2 74 259 332 2.08e-11 59.3
Msa0742250 AT4G23280.2 42.105 76 43 1 1 75 511 586 2.09e-11 59.3
Msa0742250 AT4G23280.1 42.105 76 43 1 1 75 513 588 2.09e-11 59.3
Msa0742250 AT4G23200.1 42.857 77 43 1 1 76 505 581 2.47e-11 59.3
Msa0742250 AT4G23150.1 40.789 76 45 0 1 76 505 580 2.51e-11 59.3
Msa0742250 AT1G54820.1 37.975 79 48 1 1 78 325 403 2.64e-11 58.9
Msa0742250 AT4G23290.1 40.260 77 45 1 1 76 489 565 2.96e-11 58.9
Msa0742250 AT1G54820.2 37.975 79 48 1 1 78 325 403 3.01e-11 58.9
Msa0742250 AT4G11530.1 42.667 75 42 1 1 74 521 595 3.03e-11 58.9
Msa0742250 AT4G23270.2 39.474 76 45 1 1 75 495 570 3.36e-11 58.9
Msa0742250 AT4G23270.1 39.474 76 45 1 1 75 495 570 3.36e-11 58.9
Msa0742250 AT2G05940.1 44.000 75 33 3 2 71 262 332 3.40e-11 58.9
Msa0742250 AT3G55450.2 42.353 85 38 4 2 80 279 358 3.49e-11 58.5
Msa0742250 AT3G55450.1 42.353 85 38 4 2 80 242 321 3.52e-11 58.5
Msa0742250 AT4G23270.3 39.474 76 45 1 1 75 495 570 3.60e-11 58.5
Msa0742250 AT4G23290.2 40.260 77 45 1 1 76 532 608 3.91e-11 58.5
Msa0742250 AT4G23240.2 40.260 77 45 1 1 76 197 273 3.98e-11 58.5
Msa0742250 AT1G29730.4 59.184 49 19 1 1 48 808 856 4.03e-11 58.5
Msa0742250 AT4G23310.1 38.158 76 46 1 1 75 677 752 4.08e-11 58.5
Msa0742250 AT4G23310.2 38.158 76 46 1 1 75 637 712 4.40e-11 58.5
Msa0742250 AT4G23300.1 38.462 78 45 1 1 75 522 599 4.92e-11 58.2
Msa0742250 AT4G23240.1 40.260 77 45 1 1 76 504 580 5.58e-11 58.2
Msa0742250 AT5G02290.2 44.444 72 37 2 2 71 248 318 5.73e-11 58.2
Msa0742250 AT5G02290.1 44.444 72 37 2 2 71 248 318 5.73e-11 58.2
Msa0742250 AT1G29730.3 59.184 49 19 1 1 48 840 888 6.20e-11 58.2
Msa0742250 AT1G74490.1 42.857 70 39 1 2 70 263 332 6.49e-11 57.8
Msa0742250 AT2G28930.1 42.308 78 36 3 2 74 260 333 7.09e-11 57.8
Msa0742250 AT2G28930.3 42.308 78 36 3 2 74 252 325 7.21e-11 57.8
Msa0742250 AT2G28930.2 42.308 78 36 3 2 74 249 322 7.71e-11 57.8

Find 24 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGGATCTCCTTTCGGATTC+GGG 0.260968 5_1:+62071522 Msa0742250:intron
TCAGGATCTCCTTTCGGATT+CGG 0.278473 5_1:+62071521 Msa0742250:intron
ACAAGAGAGAAAATGTCTTC+TGG 0.317288 5_1:+62071350 Msa0742250:CDS
GTTCCAAACATAAGAGTATA+AGG 0.318875 5_1:+62071565 Msa0742250:CDS
CCTTTCGGATTCGGGGTATT+CGG 0.336385 5_1:+62071530 Msa0742250:CDS
GAATATTCTGAACACATATC+TGG 0.350373 5_1:-62071384 Msa0742260:three_prime_UTR
AGAGTATAAGGAGTCACTTA+TGG 0.352614 5_1:+62071577 Msa0742250:CDS
ACTCCTTATACTCTTATGTT+TGG 0.367230 5_1:-62071568 Msa0742260:three_prime_UTR
GCTATAAATATCTGCCTTGT+CGG 0.375233 5_1:-62071093 None:intergenic
CAACATTCAGGATCTCCTTT+CGG 0.379620 5_1:+62071515 Msa0742250:intron
TAGAAGTTGCAGAGAGGAGC+AGG 0.425873 5_1:+62071157 Msa0742250:CDS
GAGTATAAGGAGTCACTTAT+GGG 0.434961 5_1:+62071578 Msa0742250:CDS
GCTCCTGAGTATGCAATTCA+TGG 0.438275 5_1:+62071062 Msa0742250:CDS
GACATGCTCTATGGTCTGCT+TGG 0.466992 5_1:-62071606 Msa0742260:three_prime_UTR
GATTCGGGGTATTCGGCCGC+TGG 0.470509 5_1:+62071537 Msa0742250:CDS
GTAGCATTAGAAATTGTAAG+TGG 0.508976 5_1:+62071125 Msa0742250:CDS
AGGATCTCCTTTCGGATTCG+GGG 0.605532 5_1:+62071523 Msa0742250:intron
TGACCATGAATTGCATACTC+AGG 0.611023 5_1:-62071065 None:intergenic
CCGAATACCCCGAATCCGAA+AGG 0.628084 5_1:-62071530 Msa0742260:three_prime_UTR
ATGTTTGGAACTTGAACCAG+CGG 0.635764 5_1:-62071553 Msa0742260:three_prime_UTR
CAGAAATAGAAGTTGCAGAG+AGG 0.636727 5_1:+62071151 Msa0742250:CDS
TTCTAATTACCCAATCAACA+AGG 0.645158 5_1:-62071190 Msa0742260:three_prime_UTR
TCATGGTCACTTAACCGACA+AGG 0.695335 5_1:+62071079 Msa0742250:CDS
GTGTTCAGAATATTCAACTG+AGG 0.709865 5_1:+62071392 Msa0742250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAAATTTGAATTTCTATC+AGG + chr5_1:62071317-62071336 Msa0742250:intron 20.0%
!! AATTTTACTACCTTGTTGAT+TGG + chr5_1:62071180-62071199 Msa0742250:CDS 25.0%
!!! ATTTTACTACCTTGTTGATT+GGG + chr5_1:62071181-62071200 Msa0742250:CDS 25.0%
! TAAAAACTAGCACAAAAATG+TGG - chr5_1:62071245-62071264 Msa0742260:three_prime_UTR 25.0%
! GAAATTCAAATTTCAAACCA+GGG - chr5_1:62071313-62071332 Msa0742260:three_prime_UTR 25.0%
! AGAAATTCAAATTTCAAACC+AGG - chr5_1:62071314-62071333 Msa0742260:three_prime_UTR 25.0%
!! TTTTCTCTCTTGTAAAACAT+AGG - chr5_1:62071343-62071362 Msa0742260:three_prime_UTR 25.0%
GTAGCATTAGAAATTGTAAG+TGG + chr5_1:62071125-62071144 Msa0742250:CDS 30.0%
TTCTAATTACCCAATCAACA+AGG - chr5_1:62071193-62071212 Msa0742260:three_prime_UTR 30.0%
!!! TGTGCTAGTTTTTATTCCAA+AGG + chr5_1:62071251-62071270 Msa0742250:intron 30.0%
!!! GTGCTAGTTTTTATTCCAAA+GGG + chr5_1:62071252-62071271 Msa0742250:intron 30.0%
GAATATTCTGAACACATATC+TGG - chr5_1:62071387-62071406 Msa0742260:three_prime_UTR 30.0%
! GTTCCAAACATAAGAGTATA+AGG + chr5_1:62071565-62071584 Msa0742250:CDS 30.0%
ACTCCTTATACTCTTATGTT+TGG - chr5_1:62071571-62071590 Msa0742260:three_prime_UTR 30.0%
GCTATAAATATCTGCCTTGT+CGG - chr5_1:62071096-62071115 None:intergenic 35.0%
! AGGGTTACTCTTTAAGAAGT+TGG - chr5_1:62071294-62071313 Msa0742260:three_prime_UTR 35.0%
AACTTCTTAAAGAGTAACCC+TGG + chr5_1:62071293-62071312 Msa0742250:intron 35.0%
ACAAGAGAGAAAATGTCTTC+TGG + chr5_1:62071350-62071369 Msa0742250:CDS 35.0%
GTGTTCAGAATATTCAACTG+AGG + chr5_1:62071392-62071411 Msa0742250:CDS 35.0%
CTTAAGCATCTCCTACATTT+AGG + chr5_1:62071451-62071470 Msa0742250:intron 35.0%
CTTCAAGCATTCTTACAACT+TGG - chr5_1:62071488-62071507 Msa0742260:three_prime_UTR 35.0%
CAAGTTGTAAGAATGCTTGA+AGG + chr5_1:62071486-62071505 Msa0742250:intron 35.0%
TTGTAAGAATGCTTGAAGGT+TGG + chr5_1:62071490-62071509 Msa0742250:intron 35.0%
AGAGTATAAGGAGTCACTTA+TGG + chr5_1:62071577-62071596 Msa0742250:CDS 35.0%
GAGTATAAGGAGTCACTTAT+GGG + chr5_1:62071578-62071597 Msa0742250:CDS 35.0%
TGACCATGAATTGCATACTC+AGG - chr5_1:62071068-62071087 None:intergenic 40.0%
CAGAAATAGAAGTTGCAGAG+AGG + chr5_1:62071151-62071170 Msa0742250:CDS 40.0%
ACTAGCACAAAAATGTGGAG+AGG - chr5_1:62071240-62071259 Msa0742260:three_prime_UTR 40.0%
GCTAGCATTATTAAGCCCTT+TGG - chr5_1:62071270-62071289 Msa0742260:three_prime_UTR 40.0%
CAACATTCAGGATCTCCTTT+CGG + chr5_1:62071515-62071534 Msa0742250:intron 40.0%
! ATGTTTGGAACTTGAACCAG+CGG - chr5_1:62071556-62071575 Msa0742260:three_prime_UTR 40.0%
GCTCCTGAGTATGCAATTCA+TGG + chr5_1:62071062-62071081 Msa0742250:CDS 45.0%
TCATGGTCACTTAACCGACA+AGG + chr5_1:62071079-62071098 Msa0742250:CDS 45.0%
! CTCACTGTTGGCCTAAATGT+AGG - chr5_1:62071465-62071484 Msa0742260:three_prime_UTR 45.0%
CTTACAACTTGGCTCACTGT+TGG - chr5_1:62071477-62071496 Msa0742260:three_prime_UTR 45.0%
TGAAGGTTGGACCAACATTC+AGG + chr5_1:62071503-62071522 Msa0742250:intron 45.0%
CGAAAGGAGATCCTGAATGT+TGG - chr5_1:62071517-62071536 Msa0742260:three_prime_UTR 45.0%
TCAGGATCTCCTTTCGGATT+CGG + chr5_1:62071521-62071540 Msa0742250:intron 45.0%
TAGAAGTTGCAGAGAGGAGC+AGG + chr5_1:62071157-62071176 Msa0742250:CDS 50.0%
CAGGATCTCCTTTCGGATTC+GGG + chr5_1:62071522-62071541 Msa0742250:intron 50.0%
AGGATCTCCTTTCGGATTCG+GGG + chr5_1:62071523-62071542 Msa0742250:intron 50.0%
! CCTTTCGGATTCGGGGTATT+CGG + chr5_1:62071530-62071549 Msa0742250:CDS 50.0%
CCGAATACCCCGAATCCGAA+AGG - chr5_1:62071533-62071552 Msa0742260:three_prime_UTR 55.0%
! GATTCGGGGTATTCGGCCGC+TGG + chr5_1:62071537-62071556 Msa0742250:CDS 65.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 62071059 62071620 62071059 ID=Msa0742250;Name=Msa0742250
chr5_1 mRNA 62071059 62071620 62071059 ID=Msa0742250-mRNA-1;Parent=Msa0742250;Name=Msa0742250-mRNA-1;_AED=0.74;_eAED=0.74;_QI=0|0|0|0.66|1|1|3|0|107
chr5_1 exon 62071059 62071202 62071059 ID=Msa0742250-mRNA-1:exon:18152;Parent=Msa0742250-mRNA-1
chr5_1 exon 62071339 62071422 62071339 ID=Msa0742250-mRNA-1:exon:18153;Parent=Msa0742250-mRNA-1
chr5_1 exon 62071525 62071620 62071525 ID=Msa0742250-mRNA-1:exon:18154;Parent=Msa0742250-mRNA-1
chr5_1 CDS 62071059 62071202 62071059 ID=Msa0742250-mRNA-1:cds;Parent=Msa0742250-mRNA-1
chr5_1 CDS 62071339 62071422 62071339 ID=Msa0742250-mRNA-1:cds;Parent=Msa0742250-mRNA-1
chr5_1 CDS 62071525 62071620 62071525 ID=Msa0742250-mRNA-1:cds;Parent=Msa0742250-mRNA-1
Gene Sequence

>Msa0742250

ATGGCTCCTGAGTATGCAATTCATGGTCACTTAACCGACAAGGCAGATATTTATAGCTTTTGCGTTGTAGCATTAGAAATTGTAAGTGGAAACAGAAATAGAAGTTGCAGAGAGGAGCAGGAATTTTACTACCTTGTTGATTGGGCCTATGTTTTACAAGAGAGAAAATGTCTTCTGGAGCTAGTTGATCCAGATATGTGTTCAGAATATTCAACTGAGGAAGTTATTGATCTCCTTTCGGATTCGGGGTATTCGGCCGCTGGTTCAAGTTCCAAACATAAGAGTATAAGGAGTCACTTATGGGAGAATCCAAGCAGACCATAG
Protein sequence

>Msa0742250

MAPEYAIHGHLTDKADIYSFCVVALEIVSGNRNRSCREEQEFYYLVDWAYVLQERKCLLELVDPDMCSEYSTEEVIDLLSDSGYSAAGSSSKHKSIRSHLWENPSRP*