AlfalfaGEDB | Msa0743170

M. sativa cultivar zhongmu-4 / Msa0743170

KEGG: -
GO: -
PFAM: PF00628.32

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0743170	XP_003617651.2	89.071	549	9	2	1	498	267	815	0.0	991

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0743170	sp\|Q5PNS0\|Y3228_ARATH	43.704	135	74	2	92	225	13	146	8.98e-34	136

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0743170	G7K8Q7	89.071	549	9	2	1	498	267	815	0.0	991

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TFs/TRs:

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Gene ID	Type	Classification
Msa0743170	TR	PHD

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0743170	MtrunA17_Chr5g0444961	91.304	437	11	1	1	410	267	703	0.0	811
Msa0743170	MtrunA17_Chr5g0444951	97.802	91	2	0	409	499	1	91	1.26e-60	194

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0743170	AT1G50620.1	34.074	270	167	4	58	319	256	522	1.71e-41	157
Msa0743170	AT3G20280.5	33.597	253	145	6	54	300	241	476	2.17e-35	140
Msa0743170	AT3G20280.3	33.597	253	145	6	54	300	241	476	2.17e-35	140
Msa0743170	AT3G20280.4	33.597	253	145	6	54	300	242	477	2.46e-35	140
Msa0743170	AT3G20280.1	33.597	253	145	6	54	300	242	477	2.46e-35	140
Msa0743170	AT3G20280.2	43.704	135	74	2	92	225	13	146	9.13e-35	136

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Find 125 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTTGGTGATGTAATTG+AGG	0.464766	5_1:+63295403	None:intergenic
AACAAACCTGCAAAGGAAAA+TGG	0.206689	5_1:+63293117	None:intergenic
AACATCTGTGGGCTGACCTT+CGG	0.439150	5_1:+63295323	None:intergenic
AACCCACTTCACCCCAGTTT+TGG	0.292600	5_1:+63294189	None:intergenic
AACCTTCACCCTGACGCCAG+TGG	0.596535	5_1:+63295118	None:intergenic
AACTGGGGTGAAGTGGGTTA+AGG	0.454696	5_1:-63294185	Msa0743170:CDS
AAGATGTCAATTTGGAGTTG+AGG	0.557635	5_1:-63293158	Msa0743170:CDS
AAGCCTTTGCCTCCAAAATA+TGG	0.319020	5_1:-63295440	Msa0743170:CDS
AAGGAGCATTCACTGATGAT+TGG	0.420539	5_1:+63295972	None:intergenic
AAGTGCTTGGGTTTAAGCGG+TGG	0.579160	5_1:-63295464	Msa0743170:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCAATGCTAGATGAAATGT+TGG	+	chr5_1:63293880-63293899	None:intergenic	30.0%
TGCTGAACAAATAGATTCTA+AGG	-	chr5_1:63293982-63294001	Msa0743170:intron	30.0%
AATCAAAAGGAATCTCATGA+AGG	-	chr5_1:63294175-63294194	Msa0743170:CDS	30.0%
TAAGCAAAAATCCACTTTCT+AGG	-	chr5_1:63294540-63294559	Msa0743170:intron	30.0%
TATTGAATGAACCTAGAAAG+TGG	+	chr5_1:63294554-63294573	None:intergenic	30.0%
ATTCACCTATGAAATTGCAA+AGG	-	chr5_1:63294679-63294698	Msa0743170:intron	30.0%
TTCACCTATGAAATTGCAAA+GGG	-	chr5_1:63294680-63294699	Msa0743170:intron	30.0%
TTAAATAGGAACAAGTCTCT+TGG	+	chr5_1:63294844-63294863	None:intergenic	30.0%
CAAGTGTGAAAATTTGTCAT+TGG	-	chr5_1:63295076-63295095	Msa0743170:intron	30.0%
TACTCTAAACAACTGTTGAT+TGG	-	chr5_1:63295157-63295176	Msa0743170:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	63293096	63296002	63293096	ID=Msa0743170;Name=Msa0743170
chr5_1	mRNA	63293096	63296002	63293096	ID=Msa0743170-mRNA-1;Parent=Msa0743170;Name=Msa0743170-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|0\|0\|1\|1\|0.75\|4\|0\|505
chr5_1	exon	63295121	63296002	63295121	ID=Msa0743170-mRNA-1:exon:18693;Parent=Msa0743170-mRNA-1
chr5_1	exon	63294702	63295040	63294702	ID=Msa0743170-mRNA-1:exon:18692;Parent=Msa0743170-mRNA-1
chr5_1	exon	63294114	63294227	63294114	ID=Msa0743170-mRNA-1:exon:18691;Parent=Msa0743170-mRNA-1
chr5_1	exon	63293096	63293278	63293096	ID=Msa0743170-mRNA-1:exon:18690;Parent=Msa0743170-mRNA-1
chr5_1	CDS	63295121	63296002	63295121	ID=Msa0743170-mRNA-1:cds;Parent=Msa0743170-mRNA-1
chr5_1	CDS	63294702	63295040	63294702	ID=Msa0743170-mRNA-1:cds;Parent=Msa0743170-mRNA-1
chr5_1	CDS	63294114	63294227	63294114	ID=Msa0743170-mRNA-1:cds;Parent=Msa0743170-mRNA-1
chr5_1	CDS	63293096	63293278	63293096	ID=Msa0743170-mRNA-1:cds;Parent=Msa0743170-mRNA-1

Gene Sequence

>Msa0743170

TTGGCAAACCAATCATCAGTGAATGCTCCTTCATGGTCAATACAGACTCAACCTGTGTCATTAGGTAGAAATGTATCAGAAAACAAGGCGCCTGCTCATAACTCTGTCAAGGTTGAAGGAACTGCTGATGCAACCGTATCAAGGGCAGGTCCACTAGTAACAGCAGCTCAGAACATTAGGCCATTCATTACTCAAACGGGACCTGGAAATATGTCTACTATGCACCAGCCGTTGCAGGGTGGGAATATGGTTCGGCCTCCTTTGATACCTAGTCACTCTGATATTGCAAAGGTTGTCCAGAAGTTGTTAATACCAAAGCTTCCTGATCATCCCACATGGACTCCTCCGTCAAGAGATTATATGAGTAAGAATTTCACATGTCAGACGTGTGAGCTCACTGTCAGTGAGGTTGATTCTGTTCTTCTCTGTGATGCCTGTGAAAAGGGATTTCACTTGAAGTGCCTGCAGCCATCAGTCATTAGAGGAATTCATAATAGAGTTGATTGGCACTGCATGAAGTGCTTGGGTTTAAGCGGTGGAAAGCCTTTGCCTCCAAAATATGGCCGTGTGATGCGATCCTCAATTACATCACCAAATTTTCCCTCTAATTCAGCTGGTATTCAACCATCTTCAGAGAAGAAACCAGATAACTTAGATCCGAAGGTCAGCCCACAGATGTTAACAACAAATGGAAACTCTGTTCCAACTGGTTCCAGTACCAATCACAATACTGAGCCGTCATTTGACTCAAATACCCCAGATACGAGAGATATACAAGGTTCCAACATTTCATCTAGCATTGAAACCATTGACGAGAAGCCTGATCCAAACATATGTGTGAAATCTGCAGCTTGTAGTGCTTCCACTGGCGTCAGGGTGAAGCTGGCTAAAAGTGGAAATTTACAATCATCACCAGGTTCTCAAGTTGAGAATGCAGTGTCACAGGACAATGCTGAAATATCATCTGATAGACACGATAGCAGTAGCTTTATTATTAGTAATCAAAAGGAATCTCATGAAGGAGAAAGTACAACTTATGATATCAAGCGTGATGACCTAGATGCTGCACAACCGAATTCTGTTAGAGGTTCTGGAACTAATACTGAAGGTATTCAGCACTGTGCTTTATCTTCAGATAGCTCACATGCTGTAGAATGGATTGGTGATGTTGTACAACTTGTAGATGAGAAAAAGCATTACCAATCTTGCTGCATTGATGGATCCATGTGGGAAGATTCCAAAACTGGGGTGAAGTGGGTTAAGGTGACAAAGTGCTACTTTCCTGATGATTTGCCGGGGAATATTGGTCATCCTTGTATATCTGAAGTCAATGAGGTTTATGAATCTAATAGTGATAGAGTTGAAATGGCTAGTTCTATCCGAGGCCCTTGTGTAGTCCTTCCATATGATAAATTTAAACAAGAAAATGACAGAAGATGTCAATTTGGAGTTGAGGCAAGTGCTAGTGTACAACCCATTTTCCTTTGCAGGTTTGTTTCTTTATCCATTGTTTTCTGA

Protein sequence

>Msa0743170

LANQSSVNAPSWSIQTQPVSLGRNVSENKAPAHNSVKVEGTADATVSRAGPLVTAAQNIRPFITQTGPGNMSTMHQPLQGGNMVRPPLIPSHSDIAKVVQKLLIPKLPDHPTWTPPSRDYMSKNFTCQTCELTVSEVDSVLLCDACEKGFHLKCLQPSVIRGIHNRVDWHCMKCLGLSGGKPLPPKYGRVMRSSITSPNFPSNSAGIQPSSEKKPDNLDPKVSPQMLTTNGNSVPTGSSTNHNTEPSFDSNTPDTRDIQGSNISSSIETIDEKPDPNICVKSAACSASTGVRVKLAKSGNLQSSPGSQVENAVSQDNAEISSDRHDSSSFIISNQKESHEGESTTYDIKRDDLDAAQPNSVRGSGTNTEGIQHCALSSDSSHAVEWIGDVVQLVDEKKHYQSCCIDGSMWEDSKTGVKWVKVTKCYFPDDLPGNIGHPCISEVNEVYESNSDRVEMASSIRGPCVVLPYDKFKQENDRRCQFGVEASASVQPIFLCRFVSLSIVF*