AlfalfaGEDB | Msa0744200

M. sativa cultivar zhongmu-4 / Msa0744200

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744200	XP_039689000.1	83.982	437	26	1	12	404	74	510	0.0	749
Msa0744200	XP_039689000.1	93.836	146	9	0	403	548	620	765	5.62e-83	285

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744200	sp\|Q9SZL8\|FRS5_ARATH	33.803	426	236	4	22	404	70	492	1.13e-75	258

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744200	A2Q1A1	50.000	154	69	2	403	548	625	778	9.36e-35	150
Msa0744200	A2Q1A1	59.817	438	128	3	14	404	81	517	1.39e-178	533

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TFs/TRs:

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Gene ID	Type	Classification
Msa0744200	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744200	MtrunA17_Chr2g0296781	40.952	315	183	3	93	406	98	410	1.13e-76	256
Msa0744200	MtrunA17_Chr1g0202341	34.579	428	237	5	17	404	43	467	1.95e-74	249
Msa0744200	MtrunA17_Chr5g0448761	33.645	428	234	5	22	402	57	481	7.51e-73	247
Msa0744200	MtrunA17_Chr6g0477261	41.333	300	173	3	108	406	2	299	1.55e-72	244
Msa0744200	MtrunA17_Chr8g0363841	33.493	418	234	8	14	390	5	419	9.37e-71	239
Msa0744200	MtrunA17_Chr5g0441581	32.783	424	247	8	16	406	20	438	1.26e-70	239
Msa0744200	MtrunA17_Chr1g0207551	31.787	431	241	8	20	406	29	450	1.15e-64	225
Msa0744200	MtrunA17_Chr2g0324011	30.211	427	259	4	16	404	1	426	8.37e-64	219
Msa0744200	MtrunA17_Chr1g0203051	35.093	322	199	4	86	404	177	491	1.87e-63	219
Msa0744200	MtrunA17_Chr3g0080351	31.220	410	245	9	20	397	71	475	2.15e-63	222

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744200	AT4G38180.1	33.803	426	236	4	22	404	70	492	1.15e-76	258
Msa0744200	AT4G15090.1	32.715	431	236	9	20	404	46	468	1.69e-65	228
Msa0744200	AT4G15090.5	32.715	431	236	9	20	404	46	468	1.69e-65	228
Msa0744200	AT4G15090.3	32.715	431	236	9	20	404	46	468	1.69e-65	228
Msa0744200	AT4G15090.2	32.715	431	236	9	20	404	46	468	1.69e-65	228
Msa0744200	AT4G15090.4	32.715	431	236	9	20	404	46	468	1.69e-65	228
Msa0744200	AT3G22170.2	29.257	417	257	7	23	406	68	479	3.91e-59	211
Msa0744200	AT3G22170.1	29.257	417	257	7	23	406	68	479	3.91e-59	211
Msa0744200	AT2G27110.2	30.303	396	251	7	24	402	48	435	2.51e-57	206
Msa0744200	AT2G27110.1	30.303	396	251	7	24	402	48	435	2.51e-57	206

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Find 89 sgRNAs with CRISPR-Local

Find 382 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAATTGGATGGATTTGA+AGG	0.422078	5_1:-64907055	Msa0744200:CDS
AAAGTGTCTTTGAAATATGC+TGG	0.421516	5_1:+64907754	None:intergenic
AAAGTTTCAAGTCATGTGTT+TGG	0.339166	5_1:-64907891	Msa0744200:CDS
AAATGAGTTTGAAGAGACTT+GGG	0.572608	5_1:-64907854	Msa0744200:CDS
AAATTGCTTCTTGTGTTAAA+TGG	0.133780	5_1:-64907094	Msa0744200:CDS
AACAATTGGATGGATTTGAA+GGG	0.574388	5_1:-64907054	Msa0744200:CDS
AAGAATCAAATCATCTGTCC+TGG	0.550254	5_1:+64907018	None:intergenic
AAGGGAGTTCTAAGAGAACC+AGG	0.594888	5_1:-64907036	Msa0744200:CDS
AAGTGTCTTTGAAATATGCT+GGG	0.568081	5_1:+64907755	None:intergenic
AAGTTTCAAGTCATGTGTTT+GGG	0.313111	5_1:-64907890	Msa0744200:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATACCAATCCCCATAAATTT+GGG	+	chr5_1:64906903-64906922	None:intergenic	30.0%
TGATACCTATCTTTCATCAA+AGG	+	chr5_1:64907508-64907527	None:intergenic	30.0%
TTGATGAAAGATAGGTATCA+CGG	-	chr5_1:64907508-64907527	Msa0744200:intron	30.0%
AACCGGCAATATGAAATAAA+GGG	-	chr5_1:64907868-64907887	Msa0744200:CDS	30.0%
GAAGAATAGATATCTCGAAA+TGG	+	chr5_1:64908397-64908416	None:intergenic	30.0%
ATTGAAATCAAAGAAAGGGA+TGG	+	chr5_1:64908537-64908556	None:intergenic	30.0%
TCATTCTCATAGTATATGTC+AGG	-	chr5_1:64908915-64908934	Msa0744200:CDS	30.0%
ACAAATGAATGGACATGATT+TGG	+	chr5_1:64908977-64908996	None:intergenic	30.0%
AAGGTAATAATTAATGCCCA+AGG	+	chr5_1:64909062-64909081	None:intergenic	30.0%
CTTTAAGTGACCAACAAAAA+AGG	+	chr5_1:64909205-64909224	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	64906886	64913270	64906886	ID=Msa0744200;Name=Msa0744200
chr5_1	mRNA	64906886	64913270	64906886	ID=Msa0744200-mRNA-1;Parent=Msa0744200;Name=Msa0744200-mRNA-1;_AED=0.35;_eAED=0.37;_QI=0\|0\|0\|0.75\|1\|1\|4\|0\|551
chr5_1	exon	64913238	64913270	64913238	ID=Msa0744200-mRNA-1:exon:19283;Parent=Msa0744200-mRNA-1
chr5_1	exon	64908728	64908973	64908728	ID=Msa0744200-mRNA-1:exon:19282;Parent=Msa0744200-mRNA-1
chr5_1	exon	64907669	64908595	64907669	ID=Msa0744200-mRNA-1:exon:19281;Parent=Msa0744200-mRNA-1
chr5_1	exon	64906886	64907335	64906886	ID=Msa0744200-mRNA-1:exon:19280;Parent=Msa0744200-mRNA-1
chr5_1	CDS	64913238	64913270	64913238	ID=Msa0744200-mRNA-1:cds;Parent=Msa0744200-mRNA-1
chr5_1	CDS	64908728	64908973	64908728	ID=Msa0744200-mRNA-1:cds;Parent=Msa0744200-mRNA-1
chr5_1	CDS	64907669	64908595	64907669	ID=Msa0744200-mRNA-1:cds;Parent=Msa0744200-mRNA-1
chr5_1	CDS	64906886	64907335	64906886	ID=Msa0744200-mRNA-1:cds;Parent=Msa0744200-mRNA-1

Gene Sequence

>Msa0744200

ATGAGGGAACCTCTCCCAAATTTATGGGGATTGGAATATGAATGTGTGCCAAATTGTGATGATGAATTAAAGCCTACAATTGGACAAGTATTTGATACGTTAGCAGAAGGTAAAAAAATTTACGAAAAATATGCACTCTCTGTCGGATTTAGCGTGCGTTCATCATCATCGACTATAGATAAAAACGGCGTGAAGCGTTGGAAGTATTTTGTTTGCTCGAAAGAGGGTTATTTGCCAAATAAGAAGGATGGTAAGGAGCAAAGTGAATCTACTGTCAAGAGACCGTTTCTCAGGTCTGCAAGGAAAGTGAATTCAGTTCACAAAAGCTTATTACATGCATGTAGTAGAGCAAATATAGGAACTTCAAAAGCTTTCCACTTATTAAAAGAACAATGTGGGGGTTATGAAAATGTTGGATGCACGCAGAGAGATCTTCAAAACTATTCCAGAGATTTAAAGACTTTGAATAAAGATTTTGATGCACATGTTTTGATAGATTACTTTAGAAGGAAGCAAGAAGTTAATCCATCCTTTTATTATGCGTATGAGGTAGGTGAAGAAGGCCGACTGAAGCATGTTTTTTGGGCAGATGGTATTTGTAGGAAAAATTATTCCTTATTTGGGGATGTGGTTTCATTTGATACTACGTATCAAACAAATAAATATCATTTGATTTTTGCACCTTTCACCGGGATAAACCATCATCGACAATCTATTACCTTCGGCGTAGGACTTATAACAAATGAGAAAAGTGATTCATTCGTATGGTTGTTTGAAAAGTTTTTGGAAGCAATGGGCGGACATAAGCCAACTCTTATAATAACCGATCAAGATCCTTCCATGAAAGTGGCTATAGAAAATATGTTTGATTCTTCTACTCATAGATTGTGCATGTGGCATATCATGAAAAAAGTTTCTGAAAAAGTAGGTGTTTCCATGAATGATGAGGATGAATTCAACAAAAGTTTCAAGTCATGTGTTTGGGGTTCAGAGACACCAAATGAGTTTGAAGAGACTTGGGTATTTTTGATGACAAGGTTTGAGTTAGAAAAGAATAAATGGTTGGCACATATGTTTGAGATTCGAAGCACTTGGATCCCAGCATATTTCAAAGACACTTTTATGGCAGGGGTATTGAGAACAACATCAAGATCAGAAAGTGAGAATTCTTTCTATGGTAATTTTCTGAATCCTAATGTTAACTTGGCTGAAGGAATACCTTGTAGACACATACTTTGTGTTTTAAAGGGAAAAAATTTCAATGAAATACCAAGTAAGCACATTGTGAATAGGTGGACTAAGTTTGCAAACAGAACACCTATTTTTGACATAGCTGACAATGTCTTGGAAGAAGACAACAAACTCATTTCTGATGTGTGGAACCACTTACATAGCTGTGTGGAGAAGGCTGGAAAAGATAAGGAAAAATTGCTTCTTGTGTTAAATGGGGTTGTTAACATGGAAAAACAATTGGATGGATTTGAAGGGAGTTCTAAGAGAACCAGGACAGATGATTTGATTCTTGGGTCCAATATTCCTGACGAAGTAGAAATCCTTCCACCAAAATTTGCAAAGACTAAAGGTAGTGGTAAAAGAATCAAAGGTGGAAAAGAGAAGGCTATTGAACAACAACAAAAAAAAAGCAATACTTTGTAA

Protein sequence

>Msa0744200

MREPLPNLWGLEYECVPNCDDELKPTIGQVFDTLAEGKKIYEKYALSVGFSVRSSSSTIDKNGVKRWKYFVCSKEGYLPNKKDGKEQSESTVKRPFLRSARKVNSVHKSLLHACSRANIGTSKAFHLLKEQCGGYENVGCTQRDLQNYSRDLKTLNKDFDAHVLIDYFRRKQEVNPSFYYAYEVGEEGRLKHVFWADGICRKNYSLFGDVVSFDTTYQTNKYHLIFAPFTGINHHRQSITFGVGLITNEKSDSFVWLFEKFLEAMGGHKPTLIITDQDPSMKVAIENMFDSSTHRLCMWHIMKKVSEKVGVSMNDEDEFNKSFKSCVWGSETPNEFEETWVFLMTRFELEKNKWLAHMFEIRSTWIPAYFKDTFMAGVLRTTSRSESENSFYGNFLNPNVNLAEGIPCRHILCVLKGKNFNEIPSKHIVNRWTKFANRTPIFDIADNVLEEDNKLISDVWNHLHSCVEKAGKDKEKLLLVLNGVVNMEKQLDGFEGSSKRTRTDDLILGSNIPDEVEILPPKFAKTKGSGKRIKGGKEKAIEQQQKKSNTL*