AlfalfaGEDB | Msa0744530

M. sativa cultivar zhongmu-4 / Msa0744530

KEGG: K13203
GO: -
PFAM: PF12874.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744530	XP_003618007.1	83.485	551	58	3	7	556	14	532	0.0	882

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744530	G7K4Z3	83.485	551	58	3	7	556	14	532	0.0	882

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TFs/TRs:

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Gene ID	Type	Classification
Msa0744530	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744530	MtrunA17_Chr5g0447971	83.485	551	58	3	7	556	14	532	0.0	882
Msa0744530	MtrunA17_Chr3g0086321	69.737	228	65	1	333	556	166	393	2.99e-102	313
Msa0744530	MtrunA17_Chr3g0086321	42.442	344	144	10	65	392	80	385	7.94e-60	203
Msa0744530	MtrunA17_Chr3g0086321	30.942	223	120	4	269	458	166	387	3.57e-15	77.8
Msa0744530	MtrunA17_Chr3g0086321	29.518	166	93	3	385	548	82	225	8.88e-12	67.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744530	AT5G61190.4	26.702	191	109	5	361	551	240	399	4.63e-12	69.3
Msa0744530	AT5G61190.4	27.315	216	113	9	365	546	298	503	1.45e-11	67.8
Msa0744530	AT5G61190.2	26.702	191	109	5	361	551	240	399	4.63e-12	69.3
Msa0744530	AT5G61190.2	27.315	216	113	9	365	546	298	503	1.45e-11	67.8
Msa0744530	AT5G61190.10	27.128	188	106	5	361	548	240	396	4.86e-12	69.3
Msa0744530	AT5G61190.10	27.315	216	113	9	365	546	298	503	1.63e-11	67.8
Msa0744530	AT5G61190.1	27.128	188	106	5	361	548	240	396	5.69e-12	69.3
Msa0744530	AT5G61190.1	27.189	217	104	9	365	546	298	495	7.98e-11	65.5
Msa0744530	AT5G61190.7	27.128	188	106	5	361	548	146	302	6.07e-12	69.3
Msa0744530	AT5G61190.7	27.315	216	113	9	365	546	204	409	1.76e-11	67.8

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Find 113 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTGCTAAGAAAATCA+AGG	0.554241	5_1:+65383428	Msa0744530:CDS
AAACAGGTTGATAGTATACA+AGG	0.585879	5_1:+65381351	Msa0744530:CDS
AAACGTAAAGCTATGGAAAG+AGG	0.547369	5_1:+65383696	Msa0744530:CDS
AAACTGTCAGATCCAAAAGT+CGG	0.650475	5_1:+65383552	Msa0744530:CDS
AAGAAGGCAGGGTTAATCAC+TGG	0.566902	5_1:+65383825	Msa0744530:CDS
AAGCTTTGCTGACTTTGGTT+TGG	0.410273	5_1:-65381131	None:intergenic
AATGCCATCATTAGACTTCA+AGG	0.445716	5_1:-65383358	None:intergenic
AATGTGCACTTTATGCAATG+TGG	0.705637	5_1:+65383740	Msa0744530:CDS
AATTGAAGTGCCTATTGCGG+TGG	0.664606	5_1:-65383601	None:intergenic
ACAAAATCAACCTGTTTCGC+AGG	0.519194	5_1:-65381507	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACACAAAAAGAATTTGCAG+CGG	+	chr5_1:65381223-65381242	Msa0744530:CDS	30.0%
ACATATAACAAACATGTTGC+TGG	+	chr5_1:65381957-65381976	Msa0744530:CDS	30.0%
CATATAACAAACATGTTGCT+GGG	+	chr5_1:65381958-65381977	Msa0744530:CDS	30.0%
GACAGAACAAGAACATTAAA+AGG	+	chr5_1:65382175-65382194	Msa0744530:intron	30.0%
ATTCAGCTAAAATGCATGTA+AGG	+	chr5_1:65382273-65382292	Msa0744530:intron	30.0%
GAAAATAAGAGAATCTTGGT+GGG	-	chr5_1:65382409-65382428	None:intergenic	30.0%
TGAAAATAAGAGAATCTTGG+TGG	-	chr5_1:65382410-65382429	None:intergenic	30.0%
GTAAGGAAAAAATCGTTGTT+CGG	+	chr5_1:65382494-65382513	Msa0744530:intron	30.0%
TAAGGAAAAAATCGTTGTTC+GGG	+	chr5_1:65382495-65382514	Msa0744530:intron	30.0%
AGATTATATATAGTTTGGCC+TGG	-	chr5_1:65382895-65382914	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	65380826	65383851	65380826	ID=Msa0744530;Name=Msa0744530
chr5_1	mRNA	65380826	65383851	65380826	ID=Msa0744530-mRNA-1;Parent=Msa0744530;Name=Msa0744530-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0\|0\|0\|1\|1\|1\|2\|0\|556
chr5_1	exon	65380826	65381998	65380826	ID=Msa0744530-mRNA-1:exon:19380;Parent=Msa0744530-mRNA-1
chr5_1	exon	65383354	65383851	65383354	ID=Msa0744530-mRNA-1:exon:19381;Parent=Msa0744530-mRNA-1
chr5_1	CDS	65380826	65381998	65380826	ID=Msa0744530-mRNA-1:cds;Parent=Msa0744530-mRNA-1
chr5_1	CDS	65383354	65383851	65383354	ID=Msa0744530-mRNA-1:cds;Parent=Msa0744530-mRNA-1

Gene Sequence

>Msa0744530

ATGCTTCTAATATTGCAGGCTGTCGGTTCATCACACGAGACTTCAGCAGCGCCTGCAAGTTTGCCTTCTGTTCAGAATGGTATTCCAATGCTTGTAATTTTGCAGTCTGCTGGTTCATCACACGTGACTTCAGCAGCACCTGCAAGTTTGCCTTCTGCGCAGAATGGTATGCCAATGCTTCTAATATTGCCGGCTGTTGGTTCGTCACACCACACTTCAGCAGCGCCTGCAAGTTTGCCTTCTGCGCGTAATGGTTCCTGGCAACTTCAAATTTATGGCAATCAGACTACAATAATGTTGCCTAACCAAACCAAAGTCAGCAAAGCTTTCAGATGTGAAGTGTGTGAAATTGATGTTAATAGCCAAGTCTCTTTTGAAAAGCACATTGCTGGAAAGAAACACAAAAAGAATTTGCAGCGGCAGACTAATCGTACAATTGCATCTCATGCAAATTTACAAACTGACACAAGTAGTATCCACGGTCAAGCGTTAATTGGCCCTGTACGCGAGCAATCAGAGCCTAAGAAACAGGTTGATAGTATACAAGGACAAGCGTTGATTGGTCCTGTAGCCGAACAATCAGAGCCTAAGAAACAGGTTGATTCTGTTAAGAATGTACAAACTGACACAAGTAGTATCCAAGGACAAGCGTTAATTGGTCCTGTCGCCGAACAATCAGAGCCTGCGAAACAGGTTGATTTTGTTAAGAATGTACAAACTGACACAAGTAGTATCCAAGGACAAGCGTTAATTGGTCCTGTAGCTGAACAATCAGAGCCTAAGAAACAGGTTGATTCTGTTAAGAATGTACAAACTGACACAAGTAATATCCAAGGACAAGCGTTAATTGGTCCTGTAGCCGAACAATCAGAGCCTACGAAACAGGTTGATTCTGTTAAGAATGTACAAACTGACACAAGTAGTATCCAAGGACAAGTGTTAATTTGTCCTGCAGCCGAACAATCAGAGCCTAAGAAACAGGTTGATTCTGTTAAGAATGTACATACTGACGCAAGTAAGATCCAAGGACAAGCATTAATTGGTCCTGTAGCAGAACAATCAGAGCCTAAGAAACAGGTTGATTCTGTTAAGGTTTGCTCAACGTGCAATGTTGTATGCGTTGGTCAAGATACATATAACAAACATGTTGCTGGGAGAAAGCATGCTGCTAAGGTTGCCTTGAAGTCTAATGATGGCATTGGTCCATCTGTTGCCGAACTCAAGCGTAACGGTGATGCTCTAATTGAAAAAGCTGCTAAGAAAATCAAGGTTGCTGAATCTGTATGGTGTGAATTTTGCAAGATTAATTGTAACAGCAAGTATAGCTACACTGCACACTTGTCCGGTAAGAAACACTTGAAGAACATGGAGAAACTGTCAGATCCAAAAGTCGGTGTTGGCAGCGGTGCCACTACCACCACCACCGCAATAGGCACTTCAATTGGGCCACAGGAAAAACCAGATTCAGATAAACCGAAAACAAAGGAGGTATCAGAACTTGATATCGAGACAGAGAAACGTAAAGCTATGGAAAGAGGAGCTGCAGTTGATGATATTAAAATGTGCACTTTATGCAATGTGGTGTGTAATAGCCAGGCAAATTTAAATACTCACCTTAGTGACCATAATCATGCTGCTATGGTGAAGAAGGCAGGGTTAATCACTGGTTGA

Protein sequence

>Msa0744530

MLLILQAVGSSHETSAAPASLPSVQNGIPMLVILQSAGSSHVTSAAPASLPSAQNGMPMLLILPAVGSSHHTSAAPASLPSARNGSWQLQIYGNQTTIMLPNQTKVSKAFRCEVCEIDVNSQVSFEKHIAGKKHKKNLQRQTNRTIASHANLQTDTSSIHGQALIGPVREQSEPKKQVDSIQGQALIGPVAEQSEPKKQVDSVKNVQTDTSSIQGQALIGPVAEQSEPAKQVDFVKNVQTDTSSIQGQALIGPVAEQSEPKKQVDSVKNVQTDTSNIQGQALIGPVAEQSEPTKQVDSVKNVQTDTSSIQGQVLICPAAEQSEPKKQVDSVKNVHTDASKIQGQALIGPVAEQSEPKKQVDSVKVCSTCNVVCVGQDTYNKHVAGRKHAAKVALKSNDGIGPSVAELKRNGDALIEKAAKKIKVAESVWCEFCKINCNSKYSYTAHLSGKKHLKNMEKLSDPKVGVGSGATTTTTAIGTSIGPQEKPDSDKPKTKEVSELDIETEKRKAMERGAAVDDIKMCTLCNVVCNSQANLNTHLSDHNHAAMVKKAGLITG*