Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748010 | sp|Q9FJK3|AGL80_ARATH | 46.795 | 156 | 82 | 1 | 1 | 155 | 1 | 156 | 2.44e-34 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748010 | G7IAC3 | 94.872 | 156 | 8 | 0 | 1 | 156 | 1 | 156 | 1.50e-95 | 282 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0748010 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748010 | MtrunA17_Chr1g0193311 | 94.872 | 156 | 8 | 0 | 1 | 156 | 1 | 156 | 2.88e-99 | 282 |
| Msa0748010 | MtrunA17_Chr5g0420351 | 85.897 | 156 | 22 | 0 | 1 | 156 | 1 | 156 | 7.12e-95 | 271 |
| Msa0748010 | MtrunA17_Chr7g0218151 | 78.205 | 156 | 34 | 0 | 1 | 156 | 1 | 156 | 1.44e-79 | 232 |
| Msa0748010 | MtrunA17_Chr4g0012761 | 76.923 | 156 | 36 | 0 | 1 | 156 | 1 | 156 | 3.99e-78 | 229 |
| Msa0748010 | MtrunA17_Chr1g0188661 | 75.641 | 156 | 38 | 0 | 1 | 156 | 1 | 156 | 1.59e-77 | 227 |
| Msa0748010 | MtrunA17_Chr1g0188681 | 69.231 | 156 | 48 | 0 | 1 | 156 | 1 | 156 | 2.40e-75 | 221 |
| Msa0748010 | MtrunA17_Chr1g0188651 | 73.718 | 156 | 39 | 1 | 1 | 156 | 1 | 154 | 8.80e-69 | 205 |
| Msa0748010 | MtrunA17_Chr1g0197461 | 63.399 | 153 | 56 | 0 | 3 | 155 | 2 | 154 | 3.32e-65 | 196 |
| Msa0748010 | MtrunA17_Chr1g0197261 | 59.355 | 155 | 63 | 0 | 1 | 155 | 1 | 155 | 1.55e-63 | 192 |
| Msa0748010 | MtrunA17_Chr1g0197321 | 58.333 | 156 | 64 | 1 | 1 | 155 | 1 | 156 | 4.96e-62 | 188 |
| Msa0748010 | MtrunA17_Chr3g0107381 | 46.104 | 154 | 82 | 1 | 1 | 153 | 1 | 154 | 1.78e-42 | 140 |
| Msa0748010 | MtrunA17_Chr3g0110131 | 50.641 | 156 | 76 | 1 | 1 | 155 | 1 | 156 | 5.30e-42 | 140 |
| Msa0748010 | MtrunA17_Chr4g0014321 | 39.355 | 155 | 92 | 2 | 1 | 153 | 1 | 155 | 1.42e-40 | 135 |
| Msa0748010 | MtrunA17_Chr4g0013041 | 39.610 | 154 | 89 | 3 | 1 | 152 | 1 | 152 | 4.45e-36 | 124 |
| Msa0748010 | MtrunA17_Chr8g0351751 | 40.881 | 159 | 90 | 2 | 1 | 155 | 1 | 159 | 4.39e-35 | 122 |
| Msa0748010 | MtrunA17_Chr5g0432691 | 37.821 | 156 | 96 | 1 | 1 | 155 | 1 | 156 | 2.16e-34 | 118 |
| Msa0748010 | MtrunA17_Chr3g0107351 | 42.208 | 154 | 88 | 1 | 3 | 155 | 2 | 155 | 6.21e-34 | 117 |
| Msa0748010 | MtrunA17_Chr4g0014031 | 42.308 | 156 | 88 | 2 | 1 | 154 | 1 | 156 | 8.95e-34 | 118 |
| Msa0748010 | MtrunA17_Chr3g0107331 | 41.558 | 154 | 89 | 1 | 3 | 155 | 2 | 155 | 1.50e-33 | 115 |
| Msa0748010 | MtrunA17_Chr3g0107361 | 40.260 | 154 | 91 | 1 | 3 | 155 | 2 | 155 | 4.23e-32 | 112 |
| Msa0748010 | MtrunA17_Chr3g0107341 | 39.610 | 154 | 92 | 1 | 3 | 155 | 2 | 155 | 1.08e-30 | 108 |
| Msa0748010 | MtrunA17_Chr4g0014131 | 43.590 | 156 | 86 | 2 | 1 | 154 | 1 | 156 | 2.97e-30 | 109 |
| Msa0748010 | MtrunA17_Chr4g0014121 | 42.949 | 156 | 87 | 2 | 1 | 154 | 29 | 184 | 7.75e-30 | 107 |
| Msa0748010 | MtrunA17_Chr3g0107461 | 35.443 | 158 | 98 | 3 | 1 | 155 | 1 | 157 | 1.08e-28 | 103 |
| Msa0748010 | MtrunA17_Chr2g0296831 | 44.628 | 121 | 66 | 1 | 1 | 120 | 1 | 121 | 1.13e-28 | 102 |
| Msa0748010 | MtrunA17_Chr2g0283941 | 37.748 | 151 | 92 | 2 | 1 | 149 | 1 | 151 | 2.72e-28 | 104 |
| Msa0748010 | MtrunA17_Chr4g0031421 | 36.508 | 126 | 78 | 2 | 29 | 152 | 1 | 126 | 2.14e-22 | 87.0 |
| Msa0748010 | MtrunA17_Chr2g0296861 | 34.194 | 155 | 101 | 1 | 1 | 154 | 1 | 155 | 3.39e-22 | 86.7 |
| Msa0748010 | MtrunA17_Chr3g0105791 | 35.714 | 126 | 79 | 2 | 29 | 152 | 1 | 126 | 8.03e-22 | 85.5 |
| Msa0748010 | MtrunA17_Chr3g0106421 | 33.333 | 129 | 84 | 2 | 29 | 155 | 1 | 129 | 1.77e-19 | 81.3 |
| Msa0748010 | MtrunA17_Chr4g0008701 | 37.903 | 124 | 69 | 3 | 1 | 120 | 1 | 120 | 1.99e-19 | 84.0 |
| Msa0748010 | MtrunA17_Chr1g0162261 | 46.237 | 93 | 49 | 1 | 64 | 155 | 22 | 114 | 5.67e-19 | 77.4 |
| Msa0748010 | MtrunA17_Chr3g0106501 | 34.109 | 129 | 83 | 2 | 29 | 155 | 1 | 129 | 1.17e-18 | 77.4 |
| Msa0748010 | MtrunA17_Chr7g0267601 | 37.168 | 113 | 67 | 2 | 1 | 109 | 1 | 113 | 2.98e-18 | 80.5 |
| Msa0748010 | MtrunA17_Chr1g0161931 | 45.000 | 100 | 54 | 1 | 57 | 155 | 21 | 120 | 2.66e-17 | 73.2 |
| Msa0748010 | MtrunA17_Chr1g0162181 | 45.556 | 90 | 48 | 1 | 64 | 152 | 22 | 111 | 3.82e-16 | 70.1 |
| Msa0748010 | MtrunA17_Chr6g0449861 | 31.818 | 110 | 69 | 3 | 1 | 104 | 9 | 118 | 1.82e-13 | 66.6 |
| Msa0748010 | MtrunA17_Chr3g0128721 | 28.571 | 133 | 89 | 2 | 3 | 134 | 6 | 133 | 2.42e-13 | 66.2 |
| Msa0748010 | MtrunA17_Chr6g0457851 | 29.091 | 110 | 72 | 3 | 1 | 104 | 11 | 120 | 2.64e-12 | 63.2 |
| Msa0748010 | MtrunA17_Chr1g0212151 | 28.182 | 110 | 73 | 2 | 1 | 104 | 1 | 110 | 1.82e-11 | 60.8 |
| Msa0748010 | MtrunA17_Chr5g0427401 | 29.221 | 154 | 85 | 5 | 1 | 148 | 1 | 136 | 9.18e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0748010 | AT5G26630.1 | 44.872 | 156 | 85 | 1 | 1 | 155 | 1 | 156 | 9.14e-38 | 129 |
| Msa0748010 | AT5G48670.1 | 46.795 | 156 | 82 | 1 | 1 | 155 | 1 | 156 | 2.48e-35 | 125 |
| Msa0748010 | AT3G05860.2 | 40.268 | 149 | 88 | 1 | 1 | 148 | 1 | 149 | 4.53e-33 | 116 |
| Msa0748010 | AT1G65330.1 | 40.909 | 154 | 90 | 1 | 3 | 155 | 2 | 155 | 5.72e-33 | 118 |
| Msa0748010 | AT3G05860.3 | 40.268 | 149 | 88 | 1 | 1 | 148 | 1 | 149 | 1.84e-32 | 115 |
| Msa0748010 | AT3G05860.1 | 40.268 | 149 | 88 | 1 | 1 | 148 | 1 | 149 | 2.81e-32 | 115 |
| Msa0748010 | AT1G65300.1 | 38.312 | 154 | 94 | 1 | 3 | 155 | 2 | 155 | 3.40e-31 | 113 |
| Msa0748010 | AT5G26650.1 | 35.948 | 153 | 97 | 1 | 4 | 155 | 2 | 154 | 2.80e-27 | 104 |
| Msa0748010 | AT5G27960.1 | 34.641 | 153 | 99 | 1 | 4 | 155 | 2 | 154 | 3.58e-25 | 98.6 |
| Msa0748010 | AT1G31630.1 | 30.464 | 151 | 103 | 2 | 3 | 152 | 2 | 151 | 1.87e-24 | 97.1 |
| Msa0748010 | AT1G22590.2 | 32.051 | 156 | 102 | 2 | 1 | 155 | 1 | 153 | 3.27e-24 | 92.4 |
| Msa0748010 | AT1G31640.1 | 34.459 | 148 | 95 | 2 | 3 | 149 | 2 | 148 | 7.70e-23 | 93.6 |
| Msa0748010 | AT5G27810.1 | 44.444 | 90 | 49 | 1 | 29 | 117 | 1 | 90 | 7.92e-21 | 82.4 |
| Msa0748010 | AT2G28700.1 | 34.746 | 118 | 76 | 1 | 1 | 117 | 1 | 118 | 4.31e-20 | 85.1 |
| Msa0748010 | AT5G26580.1 | 32.903 | 155 | 91 | 2 | 1 | 155 | 1 | 142 | 1.79e-19 | 83.6 |
| Msa0748010 | AT5G06500.1 | 36.301 | 146 | 80 | 5 | 1 | 141 | 1 | 138 | 8.38e-18 | 77.8 |
| Msa0748010 | AT2G40210.1 | 34.884 | 129 | 81 | 2 | 1 | 127 | 1 | 128 | 5.09e-14 | 68.6 |
| Msa0748010 | AT5G55690.1 | 26.582 | 158 | 95 | 3 | 1 | 151 | 1 | 144 | 3.69e-12 | 62.8 |
| Msa0748010 | AT5G55690.2 | 26.582 | 158 | 95 | 3 | 1 | 151 | 1 | 144 | 3.69e-12 | 62.8 |
| Msa0748010 | AT1G47760.1 | 33.333 | 111 | 67 | 3 | 1 | 110 | 1 | 105 | 7.50e-12 | 60.8 |
| Msa0748010 | AT5G58890.1 | 34.234 | 111 | 65 | 3 | 1 | 104 | 1 | 110 | 1.00e-11 | 61.6 |
Find 25 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAAGATGTTCCCTAGCTT+TGG | 0.376916 | 5_2:+965849 | None:intergenic |
| GCCGGATGGTTTGACCGTTT+CGG | 0.380678 | 5_2:-965755 | Msa0748010:CDS |
| TGTTTAGCCTTCTCTAGATC+TGG | 0.403006 | 5_2:+965955 | None:intergenic |
| CTTTGGTAATCCTCTGCAAT+AGG | 0.405146 | 5_2:+965866 | None:intergenic |
| AGATCTAGAGAAGGCTAAAC+AGG | 0.406038 | 5_2:-965953 | Msa0748010:CDS |
| CTCTGATGATTCGGCAAGAA+AGG | 0.409977 | 5_2:-966097 | Msa0748010:CDS |
| TTTCACCTTCTTCCTAGTCA+TGG | 0.473113 | 5_2:+966131 | None:intergenic |
| TCCGAAACGGTCAAACCATC+CGG | 0.476793 | 5_2:+965754 | None:intergenic |
| TCTGCAATAGGAAGCTCTCT+TGG | 0.511328 | 5_2:+965878 | None:intergenic |
| AGCTGGAATACCGCATAGAA+TGG | 0.529502 | 5_2:+966023 | None:intergenic |
| TTGGAAATTATAGCACAAGC+TGG | 0.550429 | 5_2:+966006 | None:intergenic |
| TTGCATTGCAAGCCATGACT+AGG | 0.554602 | 5_2:-966143 | None:intergenic |
| GCAAGCCATGACTAGGAAGA+AGG | 0.558932 | 5_2:-966136 | None:intergenic |
| TGCAGAGGATTACCAAAGCT+AGG | 0.568390 | 5_2:-965861 | Msa0748010:CDS |
| GCAGAGGATTACCAAAGCTA+GGG | 0.571791 | 5_2:-965860 | Msa0748010:CDS |
| AAACTCTTTCAAGTCCGAAA+CGG | 0.577498 | 5_2:+965741 | None:intergenic |
| GGGAACATCTTCAAAAGCAA+AGG | 0.603982 | 5_2:-965840 | Msa0748010:CDS |
| TGAGAGGTATCAAAATTCAT+CGG | 0.625826 | 5_2:-965926 | Msa0748010:CDS |
| AAGAGAGCTTCCTATTGCAG+AGG | 0.626560 | 5_2:-965876 | Msa0748010:CDS |
| AGGCTAAACAGGTGATTGAG+AGG | 0.629216 | 5_2:-965942 | Msa0748010:CDS |
| TTGATTCTAAAACAGAGGTG+TGG | 0.633348 | 5_2:-965978 | Msa0748010:CDS |
| AAGGCACGACAATCGTGAGA+AGG | 0.638977 | 5_2:-965821 | Msa0748010:CDS |
| GGTGTGGCCAGATCTAGAGA+AGG | 0.655354 | 5_2:-965962 | Msa0748010:CDS |
| GATAACAAGATTGATGCACT+CGG | 0.692256 | 5_2:-965685 | Msa0748010:CDS |
| AGCGAACTCACCATTCTATG+CGG | 0.710886 | 5_2:-966033 | Msa0748010:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACCTATAAGAAAAGAAAAA+AGG | - | chr5_2:965737-965756 | Msa0748010:CDS | 20.0% |
| !! | ACCTATAAGAAAAGAAAAAA+GGG | - | chr5_2:965738-965757 | Msa0748010:CDS | 20.0% |
| !!! | ACCCTTTTTTCTTTTCTTAT+AGG | + | chr5_2:965742-965761 | None:intergenic | 25.0% |
| !!! | AAAAAAGGGTATCATCAAAA+AGG | - | chr5_2:965752-965771 | Msa0748010:CDS | 25.0% |
| !!! | TGTTTTAGAATCAAAAGGAT+TGG | + | chr5_2:965826-965845 | None:intergenic | 25.0% |
| ! | GCTAATTGAGAAAAATATGA+AGG | - | chr5_2:966097-966116 | Msa0748010:CDS | 25.0% |
| ! | TGCTTTTATCTCTGATGATT+CGG | - | chr5_2:965704-965723 | Msa0748010:CDS | 30.0% |
| !!! | TCCTTTTGATTCTAAAACAG+AGG | - | chr5_2:965827-965846 | Msa0748010:CDS | 30.0% |
| !!! | ACCTCTGTTTTAGAATCAAA+AGG | + | chr5_2:965831-965850 | None:intergenic | 30.0% |
| !! | TGAGAGGTATCAAAATTCAT+CGG | - | chr5_2:965884-965903 | Msa0748010:CDS | 30.0% |
| TTGGAAATTATAGCACAAGC+TGG | + | chr5_2:965807-965826 | None:intergenic | 35.0% | |
| !! | TTGATTCTAAAACAGAGGTG+TGG | - | chr5_2:965832-965851 | Msa0748010:CDS | 35.0% |
| CATGAAAAACAAAAAGCTGC+CGG | - | chr5_2:966037-966056 | Msa0748010:CDS | 35.0% | |
| ! | AAACTCTTTCAAGTCCGAAA+CGG | + | chr5_2:966072-966091 | None:intergenic | 35.0% |
| GATAACAAGATTGATGCACT+CGG | - | chr5_2:966125-966144 | Msa0748010:CDS | 35.0% | |
| ! | TGTTTAGCCTTCTCTAGATC+TGG | + | chr5_2:965858-965877 | None:intergenic | 40.0% |
| AGATCTAGAGAAGGCTAAAC+AGG | - | chr5_2:965857-965876 | Msa0748010:CDS | 40.0% | |
| ! | CTTTGGTAATCCTCTGCAAT+AGG | + | chr5_2:965947-965966 | None:intergenic | 40.0% |
| TTGAAGATGTTCCCTAGCTT+TGG | + | chr5_2:965964-965983 | None:intergenic | 40.0% | |
| GGGAACATCTTCAAAAGCAA+AGG | - | chr5_2:965970-965989 | Msa0748010:CDS | 40.0% | |
| AAAAACAAAAAGCTGCCGGA+TGG | - | chr5_2:966041-966060 | Msa0748010:CDS | 40.0% | |
| ! | CTCTGATGATTCGGCAAGAA+AGG | - | chr5_2:965713-965732 | Msa0748010:CDS | 45.0% |
| AGCGAACTCACCATTCTATG+CGG | - | chr5_2:965777-965796 | Msa0748010:CDS | 45.0% | |
| AGCTGGAATACCGCATAGAA+TGG | + | chr5_2:965790-965809 | None:intergenic | 45.0% | |
| AGGCTAAACAGGTGATTGAG+AGG | - | chr5_2:965868-965887 | Msa0748010:CDS | 45.0% | |
| TCTGCAATAGGAAGCTCTCT+TGG | + | chr5_2:965935-965954 | None:intergenic | 45.0% | |
| AAGAGAGCTTCCTATTGCAG+AGG | - | chr5_2:965934-965953 | Msa0748010:CDS | 45.0% | |
| TGCAGAGGATTACCAAAGCT+AGG | - | chr5_2:965949-965968 | Msa0748010:CDS | 45.0% | |
| GCAGAGGATTACCAAAGCTA+GGG | - | chr5_2:965950-965969 | Msa0748010:CDS | 45.0% | |
| AAGGCACGACAATCGTGAGA+AGG | - | chr5_2:965989-966008 | Msa0748010:CDS | 50.0% | |
| TCCGAAACGGTCAAACCATC+CGG | + | chr5_2:966059-966078 | None:intergenic | 50.0% | |
| GGTGTGGCCAGATCTAGAGA+AGG | - | chr5_2:965848-965867 | Msa0748010:CDS | 55.0% | |
| GCCGGATGGTTTGACCGTTT+CGG | - | chr5_2:966055-966074 | Msa0748010:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_2 | gene | 965681 | 966151 | 965681 | ID=Msa0748010;Name=Msa0748010 |
| chr5_2 | mRNA | 965681 | 966151 | 965681 | ID=Msa0748010-mRNA-1;Parent=Msa0748010;Name=Msa0748010-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|-1|0|1|-1|1|1|0|156 |
| chr5_2 | exon | 965681 | 966151 | 965681 | ID=Msa0748010-mRNA-1:exon:239;Parent=Msa0748010-mRNA-1 |
| chr5_2 | CDS | 965681 | 966151 | 965681 | ID=Msa0748010-mRNA-1:cds;Parent=Msa0748010-mRNA-1 |
| Gene Sequence |
| Protein sequence |
