AlfalfaGEDB | Msa0748650

M. sativa cultivar zhongmu-4 / Msa0748650

KEGG: K04422
GO: GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0748650	sp\|F4JTP5\|STY46_ARATH	31.933	119	56	2	1	96	427	543	6.23e-14	69.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0748650	A0A396IJU2	81.429	140	3	1	1	117	1270	1409	5.33e-66	226

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0748650	MtrunA17_Chr4g0065211	81.429	140	3	1	1	117	1270	1409	1.03e-69	226
Msa0748650	MtrunA17_Chr2g0305141	68.310	142	20	2	1	117	1232	1373	2.14e-57	191
Msa0748650	MtrunA17_Chr6g0461841	57.664	137	31	2	1	110	1116	1252	6.93e-44	152
Msa0748650	MtrunA17_Chr2g0310781	57.377	122	29	1	1	99	994	1115	2.70e-40	142
Msa0748650	MtrunA17_Chr2g0310761	53.383	133	39	1	1	110	1088	1220	1.04e-39	140
Msa0748650	MtrunA17_Chr1g0196631	53.600	125	35	1	1	102	1049	1173	2.67e-39	139
Msa0748650	MtrunA17_Chr8g0357611	56.198	121	30	1	1	98	1519	1639	8.26e-39	137
Msa0748650	MtrunA17_Chr4g0072741	55.285	123	32	1	1	100	984	1106	1.08e-38	137
Msa0748650	MtrunA17_Chr5g0406251	30.075	133	68	2	1	110	432	562	6.28e-17	75.1
Msa0748650	MtrunA17_Chr2g0294351	34.375	128	58	4	1	104	605	730	8.46e-16	72.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0748650	AT3G46920.2	62.963	135	25	2	1	110	1021	1155	1.74e-48	165
Msa0748650	AT3G46920.1	68.067	119	15	1	1	96	955	1073	2.04e-48	165
Msa0748650	AT3G24715.1	59.091	132	26	2	1	104	985	1116	2.71e-43	150
Msa0748650	AT3G24715.3	59.091	132	26	2	1	104	985	1116	2.71e-43	150
Msa0748650	AT3G24715.2	59.091	132	26	2	1	104	985	1116	2.71e-43	150
Msa0748650	AT1G04700.1	57.025	121	29	1	1	98	915	1035	3.38e-41	144
Msa0748650	AT1G04700.2	57.025	121	29	1	1	98	915	1035	3.38e-41	144
Msa0748650	AT2G35050.1	51.429	140	41	2	1	117	1122	1257	1.42e-40	143
Msa0748650	AT2G35050.2	51.429	140	41	2	1	117	1122	1257	1.42e-40	143
Msa0748650	AT5G57610.1	56.198	121	30	1	1	98	930	1050	1.71e-40	142

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Find 32 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGTCAGACACTGATCTC+TGG	0.457218	5_2:+1914135	Msa0748650:CDS
AACTCTACCATGGATGGCTC+CGG	0.545721	5_2:+1914170	Msa0748650:CDS
AGGAGTTCCGGAGCCATCCA+TGG	0.578550	5_2:-1914177	None:intergenic
AGGGTTGTAGCTGTTGATTT+TGG	0.382093	5_2:-1914985	None:intergenic
AGTTGTGTGTGCAAAGAAGA+GGG	0.568974	5_2:-1915004	None:intergenic
ATCAGAGAGACCCACCTTCA+CGG	0.497305	5_2:+1914919	Msa0748650:CDS
ATCAGCAATGTCCGTGAAGG+TGG	0.486286	5_2:-1914930	None:intergenic
ATGGCTCCGGAACTCCTGAA+TGG	0.536130	5_2:+1914183	Msa0748650:CDS
CAAGGCTACTGCTTCCATTC+AGG	0.323688	5_2:-1914197	None:intergenic
CACAAGATTCAGGAATTGGA+GGG	0.613999	5_2:-1914850	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTCATATGGTATTGTGATG+TGG	+	chr5_2:1914338-1914357	Msa0748650:intron	30.0%
TTCATATGGTATTGTGATGT+GGG	+	chr5_2:1914339-1914358	Msa0748650:intron	30.0%
GCTATTATAGGTAATCCATA+TGG	+	chr5_2:1914406-1914425	Msa0748650:intron	30.0%
TGGTTGAATTCTTGAATTCA+TGG	+	chr5_2:1914426-1914445	Msa0748650:intron	30.0%
CTTCGCAAAAATATCCATTT+AGG	-	chr5_2:1914643-1914662	None:intergenic	30.0%
GTTATTGAACAGTTTCATGT+TGG	+	chr5_2:1914783-1914802	Msa0748650:intron	30.0%
GTTTCTTATTGAAGCAAAAC+AGG	+	chr5_2:1915057-1915076	Msa0748650:three_prime_UTR	30.0%
CTTCATTAAAAAGCAAATCC+GGG	-	chr5_2:1915097-1915116	None:intergenic	30.0%
CCTTCATTAAAAAGCAAATC+CGG	-	chr5_2:1915098-1915117	None:intergenic	30.0%
TTTCATATGGTATTGTGATG+TGG	+	chr5_2:1914338-1914357	Msa0748650:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	1914108	1915127	1914108	ID=Msa0748650;Name=Msa0748650
chr5_2	mRNA	1914108	1915127	1914108	ID=Msa0748650-mRNA-1;Parent=Msa0748650;Name=Msa0748650-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0\|0.5\|0.33\|1\|1\|1\|3\|91\|117
chr5_2	exon	1914108	1914230	1914108	ID=Msa0748650-mRNA-1:exon:562;Parent=Msa0748650-mRNA-1
chr5_2	exon	1914328	1914346	1914328	ID=Msa0748650-mRNA-1:exon:563;Parent=Msa0748650-mRNA-1
chr5_2	exon	1914825	1915127	1914825	ID=Msa0748650-mRNA-1:exon:564;Parent=Msa0748650-mRNA-1
chr5_2	CDS	1914108	1914230	1914108	ID=Msa0748650-mRNA-1:cds;Parent=Msa0748650-mRNA-1
chr5_2	CDS	1914328	1914346	1914328	ID=Msa0748650-mRNA-1:cds;Parent=Msa0748650-mRNA-1
chr5_2	CDS	1914825	1915036	1914825	ID=Msa0748650-mRNA-1:cds;Parent=Msa0748650-mRNA-1
chr5_2	three_prime_UTR	1915037	1915127	1915037	ID=Msa0748650-mRNA-1:three_prime_utr;Parent=Msa0748650-mRNA-1

Gene Sequence	>Msa0748650 GTTGGTGACTTGGGTCTATCCAAAGTAAAATGTCAGACACTGATCTCTGGAGGTGTGAGAGGAACTCTACCATGGATGGCTCCGGAACTCCTGAATGGAAGCAGTAGCCTTGTATCAGAGAAGGTTGATGTCTTTTCATATGGTGGTATTGTGAGCAACACTTTGCGCCCTCCAATTCCTGAATCTTGTGATCCAGAATGGAGTTTGCTGATGGAGAGATGTTGGTCCTCAGAGCCATCAGAGAGACCCACCTTCACGGACATTGCTGATGAATTGCGTTCTATGTCCATGTCAACTAAACGCCAAAATCAACAGCTACAACCCTCTTCTTTGCACACACAACTCAACAAATGA
Protein sequence	>Msa0748650 VGDLGLSKVKCQTLISGGVRGTLPWMAPELLNGSSSLVSEKVDVFSYGGIVSNTLRPPIPESCDPEWSLLMERCWSSEPSERPTFTDIADELRSMSMSTKRQNQQLQPSSLHTQLNK*