AlfalfaGEDB | Msa0750720

M. sativa cultivar zhongmu-4 / Msa0750720

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0750720	sp\|Q9SZL8\|FRS5_ARATH	35.614	570	329	10	50	584	77	643	6.45e-106	345

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0750720	A0A396GJD5	65.661	696	182	2	1	667	1	668	0.0	958

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TFs/TRs:

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Gene ID	Type	Classification
Msa0750720	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0750720	MtrunA17_Chr8g0363841	65.661	696	182	2	1	667	1	668	0.0	958
Msa0750720	MtrunA17_Chr1g0202341	32.870	648	371	17	55	661	61	685	3.18e-101	327
Msa0750720	MtrunA17_Chr2g0324011	33.158	570	337	9	55	588	20	581	1.19e-100	322
Msa0750720	MtrunA17_Chr5g0448761	31.825	663	404	14	50	674	64	716	2.60e-100	327
Msa0750720	MtrunA17_Chr6g0458391	31.746	567	313	15	52	576	231	765	5.59e-91	301
Msa0750720	MtrunA17_Chr3g0082121	32.914	556	319	10	57	577	55	591	8.52e-91	299
Msa0750720	MtrunA17_Chr2g0287651	31.429	560	334	11	55	576	85	632	2.30e-88	297
Msa0750720	MtrunA17_Chr1g0203721	32.042	568	335	12	50	577	68	624	4.36e-88	296
Msa0750720	MtrunA17_Chr5g0440451	28.293	668	400	13	50	670	48	683	4.18e-87	295
Msa0750720	MtrunA17_Chr1g0207551	29.525	674	409	14	54	689	43	688	1.01e-84	286

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0750720	AT4G38180.1	35.614	570	329	10	50	584	77	643	6.56e-107	345
Msa0750720	AT2G27110.2	30.616	552	326	9	56	579	60	582	2.07e-83	283
Msa0750720	AT2G27110.1	30.616	552	326	9	56	579	60	582	2.07e-83	283
Msa0750720	AT4G15090.1	29.194	620	384	15	1	576	1	609	4.48e-77	266
Msa0750720	AT4G15090.5	29.194	620	384	15	1	576	1	609	4.48e-77	266
Msa0750720	AT4G15090.3	29.194	620	384	15	1	576	1	609	4.48e-77	266
Msa0750720	AT4G15090.2	29.194	620	384	15	1	576	1	609	4.48e-77	266
Msa0750720	AT4G15090.4	29.194	620	384	15	1	576	1	609	4.48e-77	266
Msa0750720	AT2G27110.3	34.190	389	226	4	219	579	51	437	2.48e-75	258
Msa0750720	AT3G06250.2	28.342	561	309	12	55	577	200	705	5.49e-74	256

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Find 117 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTTCATCCAAGAGAGT+AGG	0.600823	5_2:+4248698	None:intergenic
AAACCTCTGTTTCCTTTAAT+TGG	0.141459	5_2:-4250849	Msa0750720:CDS
AAAGTTGATTATGATTGCTT+TGG	0.341170	5_2:-4250994	Msa0750720:CDS
AAATATTTGGAGTTATTTAG+GGG	0.630032	5_2:-4251232	Msa0750720:CDS
AACAAATGCCTTTGCTCAGG+TGG	0.559202	5_2:+4251330	None:intergenic
AACTTTGCAACATGCATCTA+TGG	0.373882	5_2:-4250277	Msa0750720:CDS
AAGAACAAATTGCAAAGCTA+TGG	0.512487	5_2:-4251427	Msa0750720:CDS
AAGACCGACATCATCACCAT+TGG	0.607860	5_2:+4249891	None:intergenic
AAGACTTCAGAATGGCATTG+CGG	0.687925	5_2:+4249863	None:intergenic
AAGAGGAATTTCAAAGCACT+TGG	0.487235	5_2:-4250600	Msa0750720:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAACAAATTGCAAAGCTA+TGG	-	chr5_2:4249017-4249036	Msa0750720:intron	30.0%
GACATTTGACATATTTCTCA+TGG	+	chr5_2:4249145-4249164	None:intergenic	30.0%
AATATGTCAAATGTCAAAGG+AGG	-	chr5_2:4249150-4249169	Msa0750720:intron	30.0%
AATATTTGTAACTCGCATAC+CGG	+	chr5_2:4249199-4249218	None:intergenic	30.0%
GTATGCGAGTTACAAATATT+TGG	-	chr5_2:4249199-4249218	Msa0750720:intron	30.0%
TGCACACTATAATAGAACAT+AGG	+	chr5_2:4249378-4249397	None:intergenic	30.0%
AGTTGATTATGATTGCTTTG+GGG	-	chr5_2:4249452-4249471	Msa0750720:intron	30.0%
AGAGGTTTCATTAAGTAACA+AGG	+	chr5_2:4249583-4249602	None:intergenic	30.0%
AAACCTCTGTTTCCTTTAAT+TGG	-	chr5_2:4249595-4249614	Msa0750720:CDS	30.0%
AAAAAATGCTCCTTCTCATT+TGG	-	chr5_2:4249755-4249774	Msa0750720:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	4248673	4251793	4248673	ID=Msa0750720;Name=Msa0750720
chr5_2	mRNA	4248673	4251793	4248673	ID=Msa0750720-mRNA-1;Parent=Msa0750720;Name=Msa0750720-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0\|0\|0\|0.33\|1\|1\|3\|0\|765
chr5_2	exon	4250491	4251793	4250491	ID=Msa0750720-mRNA-1:exon:1626;Parent=Msa0750720-mRNA-1
chr5_2	exon	4249581	4250399	4249581	ID=Msa0750720-mRNA-1:exon:1625;Parent=Msa0750720-mRNA-1
chr5_2	exon	4248673	4248848	4248673	ID=Msa0750720-mRNA-1:exon:1624;Parent=Msa0750720-mRNA-1
chr5_2	CDS	4250491	4251793	4250491	ID=Msa0750720-mRNA-1:cds;Parent=Msa0750720-mRNA-1
chr5_2	CDS	4249581	4250399	4249581	ID=Msa0750720-mRNA-1:cds;Parent=Msa0750720-mRNA-1
chr5_2	CDS	4248673	4248848	4248673	ID=Msa0750720-mRNA-1:cds;Parent=Msa0750720-mRNA-1

Gene Sequence

>Msa0750720

ATGGATATCAATGAATCTCAACCCGTTGAAATTGATGGGTCAGAAGAAATTTTTCTTGGTCTAGTTGTCAATAACAAAGATGAAGCTTATAACTTATACCAAGAATATGCCTTCAGAAAGGGTTTTAGTGTGAGAAAGGGGAGAGAGTTATATTATGATAACGATGAAGCTTATAACTTATACCAAGAATATGCCTTCAGAAAGGGTTTTAGTGTGAGAAAGGGGAGAGAGTTATATTATGATAACGAGAGAAAAATCACACGTTTCAAAGAGTTTTATTGTTCCAAAGAAGGGTTTAAGAATAATGAATCTGAAGGTGAAGTGTCTTATGAAAGAGTTGATTCAAGAACAAATTGCAAAGCTATGGTGAGATTTAATGTGAGCAAGGAAGGAGTGTGGAAAATCACAAAGTTAGTACTTGATCACAACCATGACTTTGTTCCACCTGAGCAAAGGCATTTGTTGCGCTCCATGAGAAATATGTCAAATGTCAAAGGAGGCCTCATAAAGTCCATGGTGAATGCCGGTATGCGAGTTACAAATATTTGGAGTTATTTAGGGGAAGAGGTCGGTGGATATAACAATCTCGGTGTAACATTGAAAGATGTGCATAATTATGTCTACAATGAAAAGCTGAAATTGATTGAAGCTGGGGATGCTCAGTCTTTAGTTAATCACTTACAAAAGAGACAAGCACAAGATCCTATGTTCTATTATAGTGTGCAGTTAGATCAAGAATCACGTTTGACCAATGTCTTTTGGAGAGATGGTAAATCTAAAGTTGATTATGATTGCTTTGGGGATGTTGTGATTTTTGACACTACTTATCGCACCAATAAGTATGACTTGATTTGTGCCCCATTTGTTGGTGTAAACCATCATTGGCAAAATGTAATATTTGGTTGTGCCTTGTTACTTAATGAAACCTCTGTTTCCTTTAATTGGTTGTTAAAAGTATTTTTGGAGAGTATGGGAAACCAACAACCAAAAACAATATTTACAGATCAAGCTGCAGCTATGGGCAAAGCAATCGGAGAAGTAATGCCAAATGCATGTCATCGTTTGTGTTTATGGCATATAGCAAAAAATGCTCCTTCTCATTTGGGATCTCTCAATTCTAATAACAAGTTTCAAAGCTTGTTTCAGAAATGCATGAAAGAATGCGACTCAGAAGAGGAATTTCAAAGCACTTGGGATGAGATAATGAATTCCTATCAACTTCATGGTCACAAATGGTTAAAAAGCATGTATGACATTCGTCACAAGTGGAGCACGGCTTTTACTAAAAATGTATTTTCTGCTGTGGCATTTGAGAAGATGGTGAAAAAGTGGCAACAATTAGAGGTTGAAAAGGAATTTAAAAATAGTCAGAGTGCACCTCCTCGCGTCATAAATAACAGTGAAACTTTGCAACATGCATCTATGGTATATACTCACAAAACCTTTAAGCTTTTTTTAAATGAGTATCTTGATGGGACAGGTGCAAGCACTTCTGTGGAGATTAGTAGATGCGATAATATGTCATATCATGAGGTAATGTTGAATAGCATACCCAATAAAAAGTATATGGTGACTTTGGACTCTTCTACCGCAACGGTCAGTTGCAGTTGTCATAAGTTTGATTCCATGGGCATTCTTTGTAGTCATGCATTGCGGGTTTACAACGTGAAAAGTATTTTAAAAATACCAGACCAGTACATCTTAAAAAGATGGTCAAAAAATGCAAGATTTGTTATTTACGACCATACAAATAAGAGCACAGAAGAAGGTTCAACTACAAGTTCAATAACCAATGGTGATGATGTCGGTCTTCTTTACCGCAATGCCATTCTGAAGTCTTATTATAATTTAGTTTTGGAAACTCAAGAGCACAAAGAAGCAAGAAAAATCATGTGGGAGTTGCTTGATACTGCAGTTCAGAAGGTTCGACAATGTATTGGCAAGCCCAATTTAAATTCAAACACAGAAAACATCAAGAACACTTTCTTGCAAAACGAGGACAATGAATGCTTGACGGTTTCTAATCCACGTCCGGCAAAGACCAAAGGAATGACTAATGTTCGTAAGAAAGGACATTTTGAGAAAAGAAAAAGATCAACTAGAAAAGCAAAAAAAGATAAAGCTAGTTTTGGTGGCCTTGGTACTGGTTTTTGTTCAGATGTGCTTGTTTGCTATTTTGCTTCTGTATGCTGCAAACTGGTGTCTCTCAGATTTCTTGTTGGTGGGACTTCAAGTATAGTGTGGATGGAACCTACTCTCTTGGATGAAGTTTTTTGGTTTGCCCAGTTGATTGGCTAG

Protein sequence

>Msa0750720

MDINESQPVEIDGSEEIFLGLVVNNKDEAYNLYQEYAFRKGFSVRKGRELYYDNDEAYNLYQEYAFRKGFSVRKGRELYYDNERKITRFKEFYCSKEGFKNNESEGEVSYERVDSRTNCKAMVRFNVSKEGVWKITKLVLDHNHDFVPPEQRHLLRSMRNMSNVKGGLIKSMVNAGMRVTNIWSYLGEEVGGYNNLGVTLKDVHNYVYNEKLKLIEAGDAQSLVNHLQKRQAQDPMFYYSVQLDQESRLTNVFWRDGKSKVDYDCFGDVVIFDTTYRTNKYDLICAPFVGVNHHWQNVIFGCALLLNETSVSFNWLLKVFLESMGNQQPKTIFTDQAAAMGKAIGEVMPNACHRLCLWHIAKNAPSHLGSLNSNNKFQSLFQKCMKECDSEEEFQSTWDEIMNSYQLHGHKWLKSMYDIRHKWSTAFTKNVFSAVAFEKMVKKWQQLEVEKEFKNSQSAPPRVINNSETLQHASMVYTHKTFKLFLNEYLDGTGASTSVEISRCDNMSYHEVMLNSIPNKKYMVTLDSSTATVSCSCHKFDSMGILCSHALRVYNVKSILKIPDQYILKRWSKNARFVIYDHTNKSTEEGSTTSSITNGDDVGLLYRNAILKSYYNLVLETQEHKEARKIMWELLDTAVQKVRQCIGKPNLNSNTENIKNTFLQNEDNECLTVSNPRPAKTKGMTNVRKKGHFEKRKRSTRKAKKDKASFGGLGTGFCSDVLVCYFASVCCKLVSLRFLVGGTSSIVWMEPTLLDEVFWFAQLIG*