AlfalfaGEDB | Msa0750870

M. sativa cultivar zhongmu-4 / Msa0750870

KEGG: -
GO: -
PFAM: PF04640.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0750870	sp\|Q1G3Q4\|RITF1_ARATH	45.652	138	74	1	18	155	3	139	6.41e-39	137

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0750870	G7K545	94.419	215	8	2	1	213	1	213	8.76e-145	411

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TFs/TRs:

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Gene ID	Type	Classification
Msa0750870	TF	PLATZ

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0750870	MtrunA17_Chr5g0395181	94.419	215	8	2	1	213	1	213	1.69e-148	411
Msa0750870	MtrunA17_Chr4g0040071	77.612	201	38	2	19	212	13	213	8.05e-113	321
Msa0750870	MtrunA17_Chr5g0411151	69.953	213	55	2	7	210	21	233	8.40e-109	311
Msa0750870	MtrunA17_Chr8g0366581	69.302	215	57	2	7	212	19	233	1.85e-105	303
Msa0750870	MtrunA17_Chr7g0265291	55.330	197	79	4	21	208	7	203	2.68e-70	213
Msa0750870	MtrunA17_Chr1g0179781	55.102	196	80	5	21	208	7	202	1.10e-69	211
Msa0750870	MtrunA17_Chr2g0308401	49.265	136	59	4	22	147	5	140	2.50e-40	135
Msa0750870	MtrunA17_Chr4g0020811	47.794	136	61	3	22	147	7	142	4.82e-40	134
Msa0750870	MtrunA17_Chr2g0297251	35.556	225	105	5	21	208	37	258	5.59e-40	137
Msa0750870	MtrunA17_Chr2g0320521	36.000	225	105	6	22	207	7	231	8.50e-40	136

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0750870	AT4G17900.1	64.115	209	67	3	7	208	20	227	1.83e-95	278
Msa0750870	AT4G17900.2	65.990	197	59	3	19	208	18	213	6.49e-93	271
Msa0750870	AT1G32700.1	66.351	211	55	5	15	212	4	211	2.46e-91	267
Msa0750870	AT1G21000.2	48.148	243	80	4	10	207	2	243	1.03e-74	226
Msa0750870	AT1G21000.1	48.148	243	80	4	10	207	5	246	1.14e-74	226
Msa0750870	AT5G46710.3	54.369	206	83	5	13	207	4	209	6.36e-73	220
Msa0750870	AT5G46710.1	53.846	208	85	5	11	207	18	225	1.24e-72	220
Msa0750870	AT1G32700.2	66.286	175	45	4	49	212	1	172	6.18e-72	216
Msa0750870	AT1G76590.1	48.246	228	77	4	21	207	18	245	1.07e-68	211
Msa0750870	AT1G43000.2	45.045	222	101	2	7	207	2	223	5.96e-65	200

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Find 68 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTTAAGTGATAGAGAT+GGG	0.507451	5_2:+4444315	Msa0750870:CDS
AAAATTGCTAAGAGGAGGAA+GGG	0.570406	5_2:+4444419	Msa0750870:CDS
AAAATTGTCTATTGTGATTG+TGG	0.499448	5_2:+4444750	Msa0750870:three_prime_UTR
AAATGTAGGAACTTTGGGAG+TGG	0.578691	5_2:-4442243	None:intergenic
AAGAAATCAAGTGGCCACCA+TGG	0.599172	5_2:+4442605	Msa0750870:CDS
AAGAACCTTGCTTGGAGAAG+TGG	0.495841	5_2:-4442634	None:intergenic
AATAAATGTTACTCATAATA+TGG	0.293213	5_2:+4444628	Msa0750870:three_prime_UTR
AATGCATACCTTACAACCAA+GGG	0.611602	5_2:-4443696	None:intergenic
AATGTAGGAACTTTGGGAGT+GGG	0.468759	5_2:-4442242	None:intergenic
AATTACATCAACAAGAATTA+TGG	0.311763	5_2:+4442388	Msa0750870:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAAAGAAAGAGGAAAATGT+AGG	-	chr5_2:4442260-4442279	None:intergenic	30.0%
CCATGTGTAGTATATGTTAA+AGG	-	chr5_2:4442444-4442463	None:intergenic	30.0%
CCTTTAACATATACTACACA+TGG	+	chr5_2:4442441-4442460	Msa0750870:intron	30.0%
TGAATGAAGAAGAAATCAAG+TGG	+	chr5_2:4442596-4442615	Msa0750870:CDS	30.0%
TGAATGCAATATGTATTGCT+TGG	+	chr5_2:4442693-4442712	Msa0750870:CDS	30.0%
CTTCACAAAATTGGATGAAA+TGG	-	chr5_2:4442929-4442948	None:intergenic	30.0%
CTTGTACACATTACTTTCTT+GGG	+	chr5_2:4443006-4443025	Msa0750870:intron	30.0%
TTGTACACATTACTTTCTTG+GGG	+	chr5_2:4443007-4443026	Msa0750870:intron	30.0%
TGCAATTCAAAAGTCAACTA+TGG	+	chr5_2:4443123-4443142	Msa0750870:intron	30.0%
ATTCAAAAGTCAACTATGGA+TGG	+	chr5_2:4443127-4443146	Msa0750870:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	4442241	4444787	4442241	ID=Msa0750870;Name=Msa0750870
chr5_2	mRNA	4442241	4444787	4442241	ID=Msa0750870-mRNA-1;Parent=Msa0750870;Name=Msa0750870-mRNA-1;_AED=0.02;_eAED=0.02;_QI=166\|1\|1\|1\|1\|1\|4\|303\|213
chr5_2	exon	4442241	4442409	4442241	ID=Msa0750870-mRNA-1:exon:1678;Parent=Msa0750870-mRNA-1
chr5_2	exon	4442562	4442780	4442562	ID=Msa0750870-mRNA-1:exon:1679;Parent=Msa0750870-mRNA-1
chr5_2	exon	4443494	4443709	4443494	ID=Msa0750870-mRNA-1:exon:1680;Parent=Msa0750870-mRNA-1
chr5_2	exon	4444281	4444787	4444281	ID=Msa0750870-mRNA-1:exon:1681;Parent=Msa0750870-mRNA-1
chr5_2	five_prime_UTR	4442241	4442406	4442241	ID=Msa0750870-mRNA-1:five_prime_utr;Parent=Msa0750870-mRNA-1
chr5_2	CDS	4442407	4442409	4442407	ID=Msa0750870-mRNA-1:cds;Parent=Msa0750870-mRNA-1
chr5_2	CDS	4442562	4442780	4442562	ID=Msa0750870-mRNA-1:cds;Parent=Msa0750870-mRNA-1
chr5_2	CDS	4443494	4443709	4443494	ID=Msa0750870-mRNA-1:cds;Parent=Msa0750870-mRNA-1
chr5_2	CDS	4444281	4444484	4444281	ID=Msa0750870-mRNA-1:cds;Parent=Msa0750870-mRNA-1
chr5_2	three_prime_UTR	4444485	4444787	4444485	ID=Msa0750870-mRNA-1:three_prime_utr;Parent=Msa0750870-mRNA-1

Gene Sequence

>Msa0750870

ATGGGGGATGGAGAAGGTGTAGTTGGAGTGAGAAGTCTGAATGAAGAAGAAATCAAGTGGCCACCATGGTTGCAACCACTTCTCCAAGCAAGGTTCTTTGTTCAATGCAAAGTGCATGCAGATTCCAACAAAAGTGAATGCAATATGTATTGCTTGGACTGTATGAATGGAGCACTTTGTTCTTCTTGTCTAGCTTCTCACAAAGATCACAGAGCTATCCAGATAAGAAGGTCTTCATATCATGATGTGATAAGGGTGTCTGAGATACAGAAATTTTTGGACATAACAGGGGTTCAAACATACGTCATAAACAGTGCAAAGATTGTGTTCTTGAATGAAAGGCCTCAGCCTAAGCCTGGCAAAGGTGTCACCAATATTTGCCATGTCTGTGAACGAAGCCTTCTTGATTCATTCACCTACTGCTCCCTTGGTTGTAAGATAGTTGGAACCTCCAAGAAATTCCAGAAGAAAAAAAAGTTAAGTGATAGAGATGGGTCAGATGGTGAAGAATCAAATAGTAATGAAAATGACATAAACAAAATTCAGAGCTTCACACCTTCAACACTGCCACCTTACAAAATTGCTAAGAGGAGGAAGGGAGTTCCACATAGAGCTCCAATAGGGGGAGTCCTTATTTACTAA

Protein sequence

>Msa0750870

MGDGEGVVGVRSLNEEEIKWPPWLQPLLQARFFVQCKVHADSNKSECNMYCLDCMNGALCSSCLASHKDHRAIQIRRSSYHDVIRVSEIQKFLDITGVQTYVINSAKIVFLNERPQPKPGKGVTNICHVCERSLLDSFTYCSLGCKIVGTSKKFQKKKKLSDRDGSDGEESNSNENDINKIQSFTPSTLPPYKIAKRRKGVPHRAPIGGVLIY*